LOCUS       DAZUMI010000001        59277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000001 DAZUMI010000000
VERSION     DAZUMI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.001"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..524
                     /locus_tag="ACG81C_000001"
     CDS             6..524
                     /locus_tag="ACG81C_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5063202.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            652..1335
                     /locus_tag="ACG81C_000002"
     CDS             652..1335
                     /locus_tag="ACG81C_000002"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5063203.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1644..1717
                     /locus_tag="ACG81C_000003"
     tRNA            1644..1717
                     /locus_tag="ACG81C_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1678..1680,aa:Arg,seq:tct)
     gene            1876..2235
                     /locus_tag="ACG81C_000004"
     CDS             1876..2235
                     /locus_tag="ACG81C_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5063204.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2358..3989
                     /locus_tag="ACG81C_000005"
     CDS             2358..3989
                     /locus_tag="ACG81C_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5063205.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4051..4692)
                     /locus_tag="ACG81C_000006"
     CDS             complement(4051..4692)
                     /locus_tag="ACG81C_000006"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5063206.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4878..4954
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4974..5282
                     /gene="rplU"
                     /locus_tag="ACG81C_000007"
     CDS             4974..5282
                     /gene="rplU"
                     /locus_tag="ACG81C_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5063207.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5309..5653
                     /locus_tag="ACG81C_000008"
     CDS             5309..5653
                     /locus_tag="ACG81C_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5063208.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5666..5959
                     /gene="rpmA"
                     /locus_tag="ACG81C_000009"
     CDS             5666..5959
                     /gene="rpmA"
                     /locus_tag="ACG81C_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5063209.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6393..9200
                     /locus_tag="ACG81C_000010"
     CDS             6393..9200
                     /locus_tag="ACG81C_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5063210.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9391..9882
                     /locus_tag="ACG81C_000011"
     CDS             9391..9882
                     /locus_tag="ACG81C_000011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5063211.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9985..10974
                     /locus_tag="ACG81C_000012"
     CDS             9985..10974
                     /locus_tag="ACG81C_000012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5063212.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11007..11810
                     /locus_tag="ACG81C_000013"
     CDS             11007..11810
                     /locus_tag="ACG81C_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5063213.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11831..12745
                     /locus_tag="ACG81C_000014"
     CDS             11831..12745
                     /locus_tag="ACG81C_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5063214.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12828..13202
                     /locus_tag="ACG81C_000015"
     CDS             12828..13202
                     /locus_tag="ACG81C_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5063215.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13341..13586
                     /locus_tag="ACG81C_000016"
     CDS             13341..13586
                     /locus_tag="ACG81C_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5063216.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13758..14822
                     /locus_tag="ACG81C_000017"
     CDS             13758..14822
                     /locus_tag="ACG81C_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5063217.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15068..15493
                     /locus_tag="ACG81C_000018"
     CDS             15068..15493
                     /locus_tag="ACG81C_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5063218.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15486..15935
                     /gene="nusB"
                     /locus_tag="ACG81C_000019"
     CDS             15486..15935
                     /gene="nusB"
                     /locus_tag="ACG81C_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5063219.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16032..16886
                     /locus_tag="ACG81C_000020"
     CDS             16032..16886
                     /locus_tag="ACG81C_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5063220.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16887..18224
                     /gene="xseA"
                     /locus_tag="ACG81C_000021"
     CDS             16887..18224
                     /gene="xseA"
                     /locus_tag="ACG81C_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5063221.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18229..18453
                     /locus_tag="ACG81C_000022"
     CDS             18229..18453
                     /locus_tag="ACG81C_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5063222.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18455..19345
                     /locus_tag="ACG81C_000023"
     CDS             18455..19345
                     /locus_tag="ACG81C_000023"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5063223.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19354..20172
                     /locus_tag="ACG81C_000024"
     CDS             19354..20172
                     /locus_tag="ACG81C_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5063224.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20279..20728
                     /locus_tag="ACG81C_000025"
     CDS             20279..20728
                     /locus_tag="ACG81C_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5063225.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20746..22428
                     /gene="recN"
                     /locus_tag="ACG81C_000026"
     CDS             20746..22428
                     /gene="recN"
                     /locus_tag="ACG81C_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5063226.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22483..22599)
                     /locus_tag="ACG81C_000027"
     CDS             complement(22483..22599)
                     /locus_tag="ACG81C_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5063227.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22672..23628)
                     /locus_tag="ACG81C_000028"
     CDS             complement(22672..23628)
                     /locus_tag="ACG81C_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5063228.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23778..24164
                     /locus_tag="ACG81C_000029"
     CDS             23778..24164
                     /locus_tag="ACG81C_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5063229.1"
                     /translation="MKKEFTFGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIK
                     APDLAIPFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFK
                     MTWRLVFLLTIVLYAVLIIVNILSYL"
     gene            24334..24765
                     /gene="mraZ"
                     /locus_tag="ACG81C_000030"
     CDS             24334..24765
                     /gene="mraZ"
                     /locus_tag="ACG81C_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5063230.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24786..25745
                     /gene="rsmH"
                     /locus_tag="ACG81C_000031"
     CDS             24786..25745
                     /gene="rsmH"
                     /locus_tag="ACG81C_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5063231.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25750..26151
                     /gene="ftsL"
                     /locus_tag="ACG81C_000032"
     CDS             25750..26151
                     /gene="ftsL"
                     /locus_tag="ACG81C_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5063232.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26152..28344
                     /locus_tag="ACG81C_000033"
     CDS             26152..28344
                     /locus_tag="ACG81C_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5063233.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28370..29332
                     /gene="mraY"
                     /locus_tag="ACG81C_000034"
     CDS             28370..29332
                     /gene="mraY"
                     /locus_tag="ACG81C_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5063234.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29346..30707
                     /gene="murD"
                     /locus_tag="ACG81C_000035"
     CDS             29346..30707
                     /gene="murD"
                     /locus_tag="ACG81C_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5063235.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30733..31821
                     /gene="murG"
                     /locus_tag="ACG81C_000036"
     CDS             30733..31821
                     /gene="murG"
                     /locus_tag="ACG81C_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5063236.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31836..33056
                     /locus_tag="ACG81C_000037"
     CDS             31836..33056
                     /locus_tag="ACG81C_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS5063237.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33212..34537
                     /gene="ftsA"
                     /locus_tag="ACG81C_000038"
     CDS             33212..34537
                     /gene="ftsA"
                     /locus_tag="ACG81C_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5063238.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34560..35801
                     /gene="ftsZ"
                     /locus_tag="ACG81C_000039"
     CDS             34560..35801
                     /gene="ftsZ"
                     /locus_tag="ACG81C_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5063239.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35818..36495
                     /locus_tag="ACG81C_000040"
     CDS             35818..36495
                     /locus_tag="ACG81C_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5063240.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36509..37108
                     /locus_tag="ACG81C_000041"
     CDS             36509..37108
                     /locus_tag="ACG81C_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5063241.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37189..37410
                     /locus_tag="ACG81C_000042"
     CDS             37189..37410
                     /locus_tag="ACG81C_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5063242.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37459..38241
                     /locus_tag="ACG81C_000043"
     CDS             37459..38241
                     /locus_tag="ACG81C_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5063243.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38262..38969
                     /locus_tag="ACG81C_000044"
     CDS             38262..38969
                     /locus_tag="ACG81C_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5063244.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39231..42029
                     /gene="ileS"
                     /locus_tag="ACG81C_000045"
     CDS             39231..42029
                     /gene="ileS"
                     /locus_tag="ACG81C_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5063245.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42078..43604)
                     /gene="zwf"
                     /locus_tag="ACG81C_000046"
     CDS             complement(42078..43604)
                     /gene="zwf"
                     /locus_tag="ACG81C_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5063246.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43619..44266)
                     /locus_tag="ACG81C_000047"
     CDS             complement(43619..44266)
                     /locus_tag="ACG81C_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5063247.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44450..44779)
                     /locus_tag="ACG81C_000048"
     CDS             complement(44450..44779)
                     /locus_tag="ACG81C_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5063248.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44956..45684
                     /gene="sfsA"
                     /locus_tag="ACG81C_000049"
     CDS             44956..45684
                     /gene="sfsA"
                     /locus_tag="ACG81C_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5063249.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45700..46713
                     /locus_tag="ACG81C_000050"
     CDS             45700..46713
                     /locus_tag="ACG81C_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5063250.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46713..47990
                     /locus_tag="ACG81C_000051"
     CDS             46713..47990
                     /locus_tag="ACG81C_000051"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5063251.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48053..50755
                     /locus_tag="ACG81C_000052"
     CDS             48053..50755
                     /locus_tag="ACG81C_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5063252.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50907..51224
                     /locus_tag="ACG81C_000053"
     CDS             50907..51224
                     /locus_tag="ACG81C_000053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5063253.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51254..51574
                     /locus_tag="ACG81C_000054"
     CDS             51254..51574
                     /locus_tag="ACG81C_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5063254.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51682..53142
                     /locus_tag="ACG81C_000055"
     CDS             51682..53142
                     /locus_tag="ACG81C_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5063255.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53210..53431
                     /locus_tag="ACG81C_000056"
     CDS             53210..53431
                     /locus_tag="ACG81C_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063256.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53462..53644
                     /locus_tag="ACG81C_000057"
     CDS             53462..53644
                     /locus_tag="ACG81C_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5063257.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53644..54057
                     /locus_tag="ACG81C_000058"
     CDS             53644..54057
                     /locus_tag="ACG81C_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5063258.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54180..55361
                     /locus_tag="ACG81C_000059"
     CDS             54180..55361
                     /locus_tag="ACG81C_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063259.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55432..55596)
                     /locus_tag="ACG81C_000060"
     CDS             complement(55432..55596)
                     /locus_tag="ACG81C_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063260.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55750..55823
                     /locus_tag="ACG81C_000061"
     tRNA            55750..55823
                     /locus_tag="ACG81C_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55784..55786,aa:Arg,seq:cct)
     gene            complement(55892..57031)
                     /locus_tag="ACG81C_000062"
     CDS             complement(55892..57031)
                     /locus_tag="ACG81C_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5063261.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57203..57337
                     /locus_tag="ACG81C_000063"
     CDS             57203..57337
                     /locus_tag="ACG81C_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063262.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57330..57965)
                     /locus_tag="ACG81C_000064"
     CDS             complement(57330..57965)
                     /locus_tag="ACG81C_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063263.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58078..58713)
                     /locus_tag="ACG81C_000065"
     CDS             complement(58078..58713)
                     /locus_tag="ACG81C_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS5063264.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58747..59208)
                     /locus_tag="ACG81C_000066"
     CDS             complement(58747..59208)
                     /locus_tag="ACG81C_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS5063265.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        19432 a        10013 c        12545 g        17287 t
ORIGIN      
        1 aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg attatatttt
       61 taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta
      121 ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga
      181 aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa
      241 aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag
      301 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
      361 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
      421 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
      481 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
      541 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
      601 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
      661 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
      721 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
      781 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
      841 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
      901 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
      961 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
     1021 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
     1081 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
     1141 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
     1201 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     1261 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     1321 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     1381 caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat
     1441 ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag
     1501 aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat
     1561 tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa
     1621 ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct
     1681 aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag
     1741 gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa
     1801 aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga
     1861 aaagaggaag aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa
     1921 tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt
     1981 tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa
     2041 ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca
     2101 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
     2161 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
     2221 tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa
     2281 atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga aaatttcatg
     2341 aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa gaattttcag
     2401 gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta gctgctgtag
     2461 cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc gcaggtttga
     2521 ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt tatcaaatat
     2581 caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca aagggaatgc
     2641 aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca ggacttttcc
     2701 gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt acttcaggta
     2761 ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac tcggaaggta
     2821 caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata aacacagcct
     2881 ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa aaatgggcga
     2941 aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg ctattgttcc
     3001 atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt aatcgtttga
     3061 atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct attagcattg
     3121 ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga atggcaaata
     3181 agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg ttgcttcctt
     3241 tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc atcaaatctg
     3301 gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc tccatgcttg
     3361 tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt ttgattgtga
     3421 cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat aaatattggt
     3481 cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat ttaagtattg
     3541 ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc gctgctatca
     3601 cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg gaaaagctga
     3661 ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca gaaaaactca
     3721 aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg cgaggcgtac
     3781 caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa gcagagcaaa
     3841 gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa ctttctcact
     3901 tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg acagctttac
     3961 atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca agactgtaaa
     4021 ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg aagctagtac
     4081 ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag ctgctggatg
     4141 aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct tttgttctgt
     4201 taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg tatattcttt
     4261 gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg gctggacctt
     4321 catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag taggtgtccg
     4381 atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag tcacgcgata
     4441 cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc cagttgaaga
     4501 aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg tatcaatctc
     4561 tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc cttgcccagc
     4621 gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta tcagttcttt
     4681 tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag aaacacaatg
     4741 cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc ttgaattttt
     4801 gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt aagctagtac
     4861 tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag cacagcatat
     4921 gctgtcactt cggatggcga gtctaagtat atcagaagga ggtgctttat agcatgtacg
     4981 caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca atctatgttg
     5041 aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc ttagtaggcg
     5101 gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa ggtactgttg
     5161 aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa aaacattcac
     5221 accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg atcaacgctt
     5281 aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc gaaatgacgc
     5341 aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt atggaagtga
     5401 tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga ttgcctcatt
     5461 agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc tttataccga
     5521 agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat tagaaaatct
     5581 tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa ttaaaacaat
     5641 taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat tatttgccca
     5701 caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac gtctaggtgc
     5761 taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc aacgcggtac
     5821 aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat ttgcaaaagt
     5881 tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt ctgtttatcc
     5941 agtagctcaa gaagcttaat tgaatcttat gattaaagag gttgtgacaa gacttttgtc
     6001 acagcctttt ttgtttataa aaataaaagt tgtagtggag gctacgaagt ttactttgtc
     6061 tttttacact taatattttt ttgccaattt ggaatcagga tgttttgata actatcgttt
     6121 tcaattgatg gaagctatat tgtttgctat cagtgaaata attatatggt aaacagtgtt
     6181 ctttttcttg tttccattat aaaaatcgta tcgggatttt tcgttctttt aaattgaaca
     6241 tttctatcta tccgcgtgtt atcaacaaaa caaaatcagg taaatagttg acgaaacaga
     6301 aataaataca tagtattatg ttttgaagcc taagtacaat agtctataga aaaggagcga
     6361 taccattggc aaaacgcatt gaccgaattt atatgtatgt caaagaaaaa accgctcatt
     6421 taacaaaggc agaatatgat caaggagtaa caacacagga aattgctgaa gtgttaggga
     6481 ttcagcgaac aaatagcagt aaagatctga atcagctagt gcgcgaagga aaactgataa
     6541 agacagacgg acgaccagta cgttacatat atcaaacact cgttacgcac gtaaagccag
     6601 ttacaaaaca tgtagtcagt tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc
     6661 aaatagatac taaggatatc tttgcaaaaa tcattggagc taacggcagc atgaaaaatt
     6721 cagtggaaca ggcaaaagct gcgattttgt atccgccaag aggattaaat tgtttgatta
     6781 caggacctac cggatcagga aagacttatt ttgcacacgc gatgtatcat tttgcaaaag
     6841 cgcatcatgt aatagatgag gaaacggaac tgattgtttt caattgtgct gattatgcca
     6901 ataaccctga gcttttgatg agtcatttat ttgggtacgc aaaaggtgct tttacaggcg
     6961 cagatgaaga aaaaatgggg attatcgatc aagcggatgg cggtatgctg tttttggatg
     7021 agattcaccg gttgccgcct gaaggtcaag aaatgatctt ttactttatg gatcatggaa
     7081 cttacagtcg tcttggggaa acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg
     7141 cgaccacaga agacccaggt tcttctctat tagaaacttt tgttcggcgt atcccaatca
     7201 atatcaaact tccggctttt gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa
     7261 tcatgattgc ccacgaagca aaccgcacgc agcgaaagat ttctttgaca gaagatgtag
     7321 caaaagcatt gatcggcagt gtgacctatg gaaatatagg acaattgaag tcaaatatcc
     7381 agttagtatg tgcacgagga tttctaaatc atatgcaatc acccgaaatc tcgatcacaa
     7441 tcgatgattt gacagaaggg attcgtagcg gattgattca attagcaagc aaccgcagtg
     7501 caatggcaga gctttctaaa atattggagc caaagataac tgtagcacca aatgatacga
     7561 tcatgaaaat ccaatcggac tcctatgagt taccttataa tctatatgat atcatcggag
     7621 ataaagcagc tttactgaag tcggatggtt tggatcaaga agcaatcaat catttcattt
     7681 ctacagatat caacatccat ctgaagtctt tctataaaga ccatggtttt tcttttaatg
     7741 cagacagcaa attagcagaa tttgtagatc cgaaaatcat agaagtgacc aatcagattt
     7801 atgtaatggt caaaagatct ttaccatatg agtttcagca aaactttatt tatgctatga
     7861 gcttgcatat cagttctttt ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg
     7921 ataacatccg tgagatggct tttgattttc cagatgaata tgctgttgcg aaagaagtgc
     7981 gtagttatat cgaaacgtat tttcaagtgg agattccaga aagtgaagac tattacttga
     8041 ccgttctact cgtctcgtta cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg
     8101 cagctcacgg aaacagcaca gcaagcagta tggtccaagt tgtccaacag ttgttagatg
     8161 cagagaacgt acgagcagta gacatgcctt tggatatgga tccgaaaaca gcattggacc
     8221 gaattgaacg caatgtacaa gaagtagatg aaggaagcgg ggtcatactc ttagtagata
     8281 tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt ccagtacgta
     8341 ctgttgatat ggtaacaact tctctagttt tggaaactgt acgaaaggtc tcagtattag
     8401 ggacagatct agatatgttg catgaatcat tgaaaaattt tcgaggttac gcagaaatat
     8461 cttctgtaga atctgttcag gaagcaaaac ctattcataa gaaaaaagct gttttggcta
     8521 tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa
     8581 acaagcgtca agaaacagaa ttagaagttc tagctttgtc gattgtggaa gtgaaagaag
     8641 aactgccagt tatccaaaaa gaatatcaga tcatagctac tactgggatt actgatttga
     8701 atatccaagc cccatttatt cctttagaac gttttattga tcaaaatatc gaagtcattt
     8761 tggatcagtt gctgatggaa tcagaattgg aggaaactga attcatctcc ctagatgaag
     8821 agtcagctaa aaatacgtgt gttgagttta tctctgataa cttcatcttt attaatggta
     8881 gtaagttaat cgatccaatg tggcaatttt ctacacaaat ctctcagacg acgggaatcg
     8941 gcgatgaaga atatggattc aaaatcaacc tagtgatgca cacagcagga atgatcgaac
     9001 gtattattcg gaatgaacca ttgactgtgg aagaaaacga attgacaaat acaacgaatg
     9061 atcctcttta tagccaattg gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa
     9121 aagtgccgat agaagaaatg tattatctgc tacgtctagt ccataatcag ttagataaaa
     9181 aagaatacac tgtgccatga atacacaaaa acgatacact tttatagtgt atcgtttttg
     9241 tgtattggtt aagaatcctt taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt
     9301 ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata ggggtttctg
     9361 tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg tatagatgat
     9421 cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc
     9481 attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact aaaagaagct
     9541 gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca aatctatcat
     9601 agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca agatgttgaa
     9661 gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat gagttttact
     9721 gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat tgattccttt
     9781 cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc tgacaggaaa
     9841 atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa
     9901 aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa tttgttacaa
     9961 caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca tggacaattt
    10021 gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc
    10081 gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga agaagcggtt
    10141 gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg cggaacaccg
    10201 ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat cgtcagcggt
    10261 ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga atctgctcat
    10321 gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa accggaagaa
    10381 ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa aggagcactt
    10441 ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg tgttgactca
    10501 cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt
    10561 atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat cgaacaagct
    10621 gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga agtagcaaaa
    10681 gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga agacgtattg
    10741 acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg
    10801 gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt tgaagcgttt
    10861 gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa tgattcacgc
    10921 gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc gtaataaata
    10981 atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta gtcgtattag
    11041 ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat caaccactag
    11101 tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc gtacttggcg
    11161 gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta gcaccagatg
    11221 ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa ggtgttaaag
    11281 gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg ttcttaacaa
    11341 tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct gctgaagaag
    11401 gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa ggggttcgta
    11461 tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa tcattccttg
    11521 aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg gtcgtagctg
    11581 taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca ttcttcgtta
    11641 ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt gctttaggtg
    11701 ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca aataatggag
    11761 gcggaagcaa ttctggtgac cctttaggcg acattttgaa tgactattaa tcttgaagga
    11821 ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc
    11881 gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa acggtggctg
    11941 ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag aacgttctgc
    12001 agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt ctccaatttt
    12061 aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc
    12121 gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg acccagtgtt
    12181 ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta tgggaggaaa
    12241 tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg cattcatgtg
    12301 gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc tttctggcgg
    12361 attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat tagctgccct
    12421 tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa aattagataa
    12481 aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca agagtgcatt
    12541 tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat tacaagacaa
    12601 cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat gtatgtggtt
    12661 gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag tcggtgttgt
    12721 tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt gtcccgagct
    12781 ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg gttcaatcaa
    12841 tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg acagattacg
    12901 ggcaaatcat gataggtaat aatggttttg agttttacca tgcgcgagat agacgcaaat
    12961 atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg ctaaagggaa
    13021 aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca ttttcttcca
    13081 aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat agaatggtac
    13141 aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt
    13201 aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt gcagaagaaa
    13261 atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta aaattgatta
    13321 gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga gccgtgatcg
    13381 gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta acgaatatca
    13441 ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg ggaccaaaag
    13501 ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta gcagttgtct
    13561 catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa aggagttgca
    13621 gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt tttccggaaa
    13681 tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag
    13741 aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag
    13801 aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta acgaacttta
    13861 caggtacgac aggattagct gttattactt tggaaaaagc attttttatt actgattttc
    13921 gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa aatgtcggac
    13981 ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca ttagcttttg
    14041 aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc gatgcggaat
    14101 tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg
    14161 ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc ctgaaaatga
    14221 tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat atgcgttctc
    14281 ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt tcggctatgc
    14341 cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca ttggactttg
    14401 gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt ggtgatcctg
    14461 gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca gttattgatg
    14521 ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat tatatttcaa
    14581 aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt ttagagatcc
    14641 acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca
    14701 ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag gatgacttgt
    14761 tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg atcatcttat
    14821 agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt gttatagaaa
    14881 atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga taataaagtt
    14941 gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt cttaccacgt
    15001 gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca aagggaggaa
    15061 taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt taggagagat
    15121 cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta aagtagaagg
    15181 cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag gacgtgcagc
    15241 tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag atctttactg
    15301 ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg aacgggtcag
    15361 ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc atgtagtagc
    15421 tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag acgaagagga
    15481 agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg caggctttat
    15541 ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac gcaatcgaat
    15601 tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt tatcttgata
    15661 cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt
    15721 taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc ttgcgtatcg
    15781 cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta aatgaagcaa
    15841 tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac ggtgttttat
    15901 ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc tatgaatcat
    15961 cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa aaaatatctg
    16021 ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag
    16081 agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga ttagtcgttt
    16141 tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa
    16201 aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct gaagacgaac
    16261 tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata ttggtccaat
    16321 taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc cctgaaaaag
    16381 acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa ccggacaaga
    16441 ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc gatccggcgg
    16501 gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg gctcagctac
    16561 tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat ctggctgaac
    16621 tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt gtcacaaaag
    16681 aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat gctaacggta
    16741 agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg atcactcctg
    16801 tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc gtaaaatctg
    16861 ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc actacattaa
    16921 cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta tatttaacag
    16981 gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc ttaaaagatg
    17041 accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta cgattccagc
    17101 caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt
    17161 cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta tatcaagaat
    17221 tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc
    17281 tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg
    17341 atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg ttcccaacag
    17401 ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga gtagaagaga
    17461 tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag gatttatggc
    17521 cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg gtgatttcat
    17581 ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt cgggctgcca
    17641 ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg atgcgcattc
    17701 aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac
    17761 ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc
    17821 agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag caaatttatc
    17881 atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg gctccgcttt
    17941 atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta gaagagaaaa
    18001 tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa tctcttgatt
    18061 tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta aatgatcaag
    18121 tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat tatcaagatg
    18181 gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat ggaaaatcca
    18241 acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga acaaggagac
    18301 gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag caaacattgc
    18361 caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa
    18421 gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact tttcagctat
    18481 tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata caacggatag
    18541 aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa tacgtccctt
    18601 attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg tctatcaagt
    18661 agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt tgcctgcgat
    18721 ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg gcgaagcatt
    18781 agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca gtatggcaca
    18841 cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg cgggatcaca
    18901 aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga
    18961 agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg ccttgctggc
    19021 tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac gattagccga
    19081 acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg gtacaacgaa
    19141 aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt acccgcgttt
    19201 attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg ccaacaagat
    19261 catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc gccagatgat
    19321 caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt agatgtatta
    19381 gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt gatggctggt
    19441 ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat tccagtgagt
    19501 agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg attaaaatta
    19561 gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt agatattggt
    19621 gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct
    19681 ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag agtcgtcgta
    19741 atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg aattccagaa
    19801 gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc attacatgct
    19861 attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga agcaggcaga
    19921 gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa agttttagaa
    19981 gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag ttattctccg
    20041 atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt acccgaaagc
    20101 ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca cgaacagttt
    20161 gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt cttctttatt
    20221 ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg aagacgtaat
    20281 gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag atatccgaaa
    20341 gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc aagctacgat
    20401 ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg gcggctaccg
    20461 ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa aactattaaa
    20521 agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa cattgccggg
    20581 aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat tattttcgat
    20641 tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg cagttttatt
    20701 gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct acaagaaatc
    20761 agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga aggaatgacc
    20821 gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt aggtctgtta
    20881 gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa
    20941 gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga attagggatc
    21001 gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg aaaaaatgtt
    21061 tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg cagttattta
    21121 gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc acatcttgct
    21181 ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta tcagcaagaa
    21241 tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc
    21301 tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc tgctgatttg
    21361 caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa
    21421 attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca aaacagtgta
    21481 gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga tgtggagtat
    21541 gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc aatcggcgat
    21601 atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga agttacgcaa
    21661 cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga gtcaatattg
    21721 gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga aggtcaattg
    21781 gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca agccgcagat
    21841 cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct gcatgaattg
    21901 aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga agaaaataga
    21961 caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa tccaggagag
    22021 ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat gttgcttgct
    22081 ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga tgaagtggat
    22141 acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca aatatcgaaa
    22201 tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc cgactatcaa
    22261 tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc agagttgaaa
    22321 acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat tacaaaactc
    22381 acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag
    22441 tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga cactcattac
    22501 agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga caaattttgt
    22561 tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc tcgtcattaa
    22621 ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt tttataaaaa
    22681 cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta atagaactag
    22741 ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc catagatact
    22801 gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt tcataatatt
    22861 attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta ctagttttaa
    22921 ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac gtaaatgatt
    22981 tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca atgcatctaa
    23041 attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca tgatttgata
    23101 aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc attgagattg
    23161 aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt gaaagaagat
    23221 attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt tctttaaaaa
    23281 cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa taatagagag
    23341 cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag gatattgtaa
    23401 caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac gggaattttc
    23461 tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat gtgtcagctg
    23521 atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt cagtttcctt
    23581 tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga ttttcctcct
    23641 gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat gtgtttgtaa
    23701 gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa agtatataca
    23761 gtatacttga agcaacgata aaaaaggagt ttacgttcgg cacattcaca ccacttatct
    23821 tgttttggta tatttacccg gtaatcgttt tgtttggttg ccaatttctt gtaaaattat
    23881 tttcgttaaa tcgtcgtttt aaaataaaag cccctgattt ggcgattcct tttttgtggg
    23941 gcggtatgca tgcattgtct agaaatacag ggacaatttc tatcttaccg tttttcttta
    24001 tttctgtttt gctaatggga atttgtattg ctgttttcca agcatattat tatgaagaaa
    24061 ttatttatcc gcgttatttc aagatgactt ggcgtttggt ttttctgttg acaatcgtct
    24121 tgtacgctgt cttgatcatt gtgaacatac tttcttacct atgaaaaaaa tagaaaagct
    24181 gtaaaaagtc ttttaaaagg ctttttgcag cttgattttt aaaaaaatgt ttatgaataa
    24241 aaaaagttgt tcccggattt cccagaaaag gtggtagaaa gtgggggatt gtggtagact
    24301 aattttagtt agtggaggaa tggggggctt tcaatgttga tgggtgaatt ccaacataat
    24361 attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc gtgaagaact tggcgaaaag
    24421 tttgttctga cacgcggatt ggatggctgt cttttcggct atccgatgag cgaatgggaa
    24481 aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa aagacgcacg tacttttgtg
    24541 cgattcttct attcggctgc cactgaatgc gaacttgata aacaaggacg aatcaatata
    24601 cccagcacgt taagaaatta tgcagctttg acaaaaggtt gcgtagtcat cggtgtatct
    24661 aaccgaattg aaatttggga tgagacacga tggcaagatt tttctgcagc agcagaagag
    24721 aactttgacg aaattgctga aagtatgatt gatttcggac tataagaata ggaagaggct
    24781 gactaatgac tgaaacattt caacactata cagtcatgtt gaaagaaacg gtagatggat
    24841 tgaacatcaa accggatggc gtttatgtag actgtacact cggcggtgcc ggacatagtg
    24901 aatatttatt aagccaacta agttctaaag ggcatctgta tgcgtttgac caagatcaaa
    24961 aagcaatcga tcacgccaag ctgcgtttag cttcttatgt cgaacaaggt atggtcacat
    25021 ttatcaaagc gaacttcaga gaattagaag aacgcttgag tgagcatgta gataaggtag
    25081 acggcatttt gtatgatcta ggggtttcct ctcctcagct agatgaagca gaacgtggat
    25141 ttagttatca tcaagatgca ccgttggata tgcgcatgga tcagtcagct cctttatccg
    25201 cttatcacgt aattaatgaa tattcctacc atgaattagt aaagattttc ttccgctatg
    25261 gcgaggagaa attttccaaa caaattgctc gtgaaatcga acgagtacga aaaacaaaac
    25321 caatccaaac aactggtgag cttgtcgaaa tcattaaaca agtcatccca gctcccgcaa
    25381 gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc tgtgcggatt gcagtaaatg
    25441 acgaactcgg tgccgaagag gcctctttag aacaggcaat ccggttattg aaagtcaatg
    25501 gaagaatcag cgtaatcaca ttccattcat tggaagacag aattgtcaaa agtatgttta
    25561 aagaatatag tacggtacaa gatctaccac cgggacttcc tgtcatacct gacgaattcc
    25621 aaccagaact gaaagtcatt actcgtaaac caatccttcc ttcagaggaa gagctaacag
    25681 aaaacaatcg atcacgcagt gcaaagttac gtattgcaga aaaaataaga atgaaagagg
    25741 agtagaatga tggcagaatt aaagaaaatg caagattacg aatatgattt acctgaattg
    25801 gtggatcagc cggaacaaca aatgcctgat tcttcttcga atcaagaaat cattcttcca
    25861 caatcaccgg caaaacgatt aaagcagatt tctgctttgg aaaaaattgc tgtggcaagc
    25921 gtggcgattg ctattgttgc tttatgtgtt ttaacagtta tgctgcgcac aaatatcagc
    25981 ggagctgaaa aagaaatcac gtcaatacaa acagaaatca atgataaaaa gcaagaaaaa
    26041 acgagtttag aacaagaaaa aaacgaactt tctcgtactg aacggattaa acaaatcgca
    26101 gaagaaaaag gattgtctat taatgacgac aatttaagga aagtgaagta aatgagtctt
    26161 aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa atccgatgaa caatcgcaaa
    26221 aaagtaggta tcattttatt tgctacgagt attggattgt tctttttatt tgccgtccgt
    26281 ttctcgtata tcgttattgg aggtcatgtt gctggtacct cattagctga aaaaacaaaa
    26341 cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag gaacgattta tgatcgtaac
    26401 ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta aagcgatact ctcaaaaaca
    26461 tatacctctg gggataaaaa actgtatgtt gaagaaaaga attttgataa aatagcagaa
    26521 atcctgcata agaatctttc tatagacaaa aaagatgcac tgaatatttt agaggacggc
    26581 gcaaaaaaag agctgtatca agttgaattt ggtagctatg ggaaaaatat ctcccaagaa
    26641 accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg tcgctggtct ttattttgtc
    26701 gaccatcagg ctcggatgta tccaaatggc gtcttttctt ctcattttat tggatacgca
    26761 gttccagaca aagatgagaa tggcttagtt ggtaaactag gtttggagtc tgcttacaat
    26821 gatatcttga gcggtaaaga tggtaagatt atttatcaaa aagataattt tcaaaatcct
    26881 ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg gacaagatat ctacacgact
    26941 ttagacagca gactacaaag ttatttagaa acactgatgg atcaagtaaa tgaagaatat
    27001 cagccagaag aattgacagc agtcctgatg aaagcaaaaa ccggagaaat cttggcaatg
    27061 ggacaacgac ctactttcaa tcctgaaaca atggaaggat tgactggaaa agatgctatc
    27121 tggcgtaatt tcttggtaca agatagttat gaaccaggat caaccatgaa agttttcact
    27181 actgcagctg ctatcgaaga aggagaattc aacgagaatg aaaccttcca atctggaaaa
    27241 atccaagtag aagatgctac gatcaatgac catgactttg gtgaaaaagg tgtgctgacc
    27301 atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg tcatactgga acagcgatta
    27361 ggcggacgat ggtataacta tttacaaaaa ctgggatttg gacaaagtac tcattctgga
    27421 ttagatgatg aagtaaacgg tgctttacct acattaaata ttgtcgaccg agcaatgagt
    27481 gcttatggac aagcggtagg tgtgacgaat ttccaaatga tgaaagcttt cacttctatt
    27541 gcgaataacg gtacaatgat tcaacctcga tatatcagca aagtggtgga tccccaaact
    27601 ggagaagagc gaacaactca aacagaagtt ctagggcaac ctttttcaaa agaaacaaca
    27661 gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg aaaactacgg tagtgcttat
    27721 ggtgtatata gtgtacctgg atacaatgta tcggcaaaaa acggaacagc ccaaatcgct
    27781 tctgatacag ggggatatca aacaggagat acagcctacc tgtattcgat tgtggagatg
    27841 gttccttctg aagatccaga ctatgtcttg tatctaacaa tgaaacaccc taagacatat
    27901 gatcgaatgg cattggctaa aatcgcaaat ccattgatga aacgggcaat ggattttaaa
    27961 gaaactgaag aagattcaga tacagaaaca aaaacagaaa aagtatccgt agccgattat
    28021 cgtaatcttg aagcggacgt tgcagcagct gatgctcaaa aaagtggact gcaacctgta
    28081 gtgatcggta atggtaaaaa agtacaaaaa caatctactg ccaacggtga tcagttgatc
    28141 tctggagaaa aactgatcct atatactggt ggtgataagc tgatgcctga cgtaacagga
    28201 tggtcaaaag cagatatcat gaagctgggg aaaatcctcg gtattgaagt tagttttgat
    28261 ggagatggtt attgtgtcaa acaagaactt gctccatatg aaaaaatcac tgaagacaaa
    28321 cttagtttta ctttagaaga ataagaagag aaataggaga gaaaagagca tggaatggac
    28381 acaagcattg attcccattg tcagcagttg tgcaatgaca attgccgcaa tgccgctatt
    28441 tattggttat tttcaaatga aaaagcaagg tcaggcgatt cgtgaggagg gaccaaagtg
    28501 gcacaatagc aaagccggaa ctcctacaat gggcggctta gtgtttctga tcggctctat
    28561 tttgacagga atttgggttg gagcttggca aaaacagcta actcctactc ttttcatctt
    28621 gctttttgta ttagcgttat acggagtaat cggctttctt gatgatttta taaaaatatt
    28681 caagaaaaga aacatgggac tgaactcgaa gcagaaattg attggacaga ttctaggggg
    28741 aatcatcttt tatcttgttt accgttctga aggttatcca ggtgtattga atttcttcgg
    28801 actagatcta cctttaggaa tcatttatgg tattttcgct ctcttctggc ttgttggatt
    28861 ttcaaatgca gtaaatctga cagatggcat cgatggatta gttgctggct taggaagtat
    28921 ctcttttgca gcttacggtc taattgcttg gcatcagcat cagtatgatg tattaatcat
    28981 ttgcttaagt gttttaggag gtttactggg tttcttcgtc tacaacagaa aacctgccaa
    29041 gatttttatg ggagatgtcg gttcattagc gttgggcgga cttttagcgg caatttctat
    29101 aatgctaaac caagaatgga cattgctttt gattggtttg atctatgtta tggaaacagc
    29161 aagtgtgatg cttcaagtta cttcctttaa gcttacagga aaacgaattt ttaaaatgtc
    29221 tccaattcat catcactttg aaatgtgcgg atggtcggag tggaagattg atacagtttt
    29281 ttggttgatc agcattgtga cttcgttgat cacattatgg atcgtttggt aggagatgac
    29341 gggaaatgaa aaaaatatca acatatgaaa ataagaaagt gcttgtctta ggactcgcaa
    29401 aaagcggagt aagtgcggcc aaattattac atgaattggg cgcacttgtc accgttaatg
    29461 atggaaaacc ttttgacgaa aatccggaag cacaagagct gctttcttta gggataaaag
    29521 taataacagg tagtcatcca atcgaattgt tggatgaaga attttcattg atggtcaaaa
    29581 atccagggat tccctacagt catcctttag tagcaaaagc ccaagaaatg ggtattccgg
    29641 taattacgga agttgagcta gcttacgaag tagcggaatg cccaattatt gggattacag
    29701 gaacgaacgg aaaaacaacg acaacaacga tgacaggact actactgaat gcaggagcag
    29761 atcaaggaat tgcacgttta gcgggtaata ttgggtatcc agcaagtggt gtagcgcaag
    29821 aagcaaaatc agaagataaa attgtgatgg aattgtctag cttccaactc atgggaatca
    29881 cagatttccg ccctcatata gcggtgatta ccaatatcta tgaagcacat attgattatc
    29941 atggtacaag aaaagaatat gtcaaagcta aatggaacct tcaaaaaaac atgacagaaa
    30001 aagattatct gatccttaac tggaatcagt cagaactaca agaattagcg caaagaacaa
    30061 aagcacgcgt actgcctttc tcgacaaaag aagtgttgga agacggtgtg tatgctgacg
    30121 attactcaat atactataaa aaagaaaaaa taatggaaat atctgaactt ggcgtaccag
    30181 gtaagcataa tgtagaaaat gcactagctg ctatttctgt agcaaaattg tatggtatat
    30241 caaatgaagc tatacgagaa acgttgcatt ttttccatgg cgtaccacat cgtacgcaat
    30301 atgtaggaga aatccaaggc agaaaatttt ataatgattc aaaagcaacc aatattttag
    30361 cgactaaaat ggctctaagt ggatttgaaa cgtctaaggt cgtgttattg gctggcggat
    30421 tagacagagg aaatacattt gatgaattaa ttccttcatt aaaaggaatc aaagcaatgg
    30481 tcgtatttgg ccaaacaaaa gaaaagttga tggatgctgg aaaaaaagca ggcatcgaga
    30541 caatagtaac agccgattct gtagaacaag cagttccttt agcattagaa aacagtacag
    30601 atggtgatgt agttttatta tcaccagcga atgccagctg ggatcaatac ccaaattttg
    30661 aaactagagg aaacagattc atggaagctg tgaaccgatt aaaatagaaa aagtagaagc
    30721 gagcgataaa aaatgaaaat tttggttaca ggcggaggaa cagggggaca tatttatcca
    30781 gctcttgcct ttgtcaatta tgtaaagact caagaaccga atgctgaatt catgtatgtg
    30841 ggagcaaaac gaggtctaga aaacaaaatc gtccctgata caggaatgcc tttccatacc
    30901 ttggagattc aaggatttaa acgaaaaatt tccatgcata acatcaagac ggtccgattg
    30961 tttcttaaga gtatccgtca agcaaaaaaa atcctcagtg attttcaacc agatatcgtt
    31021 atcggtactg gaggatatgt ttccggagcg gtagtgtacg ctgcagccaa aatgggtatt
    31081 ccaacagtta tccatgaaca aaacagtgta ccaggtgtta ccaataaatt tttaagccga
    31141 tatgtggatc ggatcgctct ttcatttaaa gatgcggctt ccttttttcc tgaaaacaaa
    31201 gcagttcttg taggtaatcc tcgagcacaa gaagtagcag atactaaaaa atcagatatt
    31261 ttgaaaacat ttgatttgga tccagaaaag aaaacagtct tgatttttgg cggtagtcaa
    31321 ggagcactta aaatcaatca agcagtatca gtttttttaa gtgaatatat tccttcttct
    31381 taccaagttt tgtatgcttc aggagataga tattatgaag agatcctaaa taaaataaat
    31441 cctgaaatac aaaatattag tgtccagcct tatatcaaaa atatggcaga ggtcatggct
    31501 tccagtgatc tgctggtggg gcgtgctggt gcaacttcga tcgcagaatt cacagctctg
    31561 gggttaccgg cagtgttggt tcccagccca tatgttacga atgatcatca gactaaaaat
    31621 gcaatgagtc ttgttcacgc aggagcagca aaaatgattg cagataatga actaacaggt
    31681 gaatcactaa gtcagacagt caacgaaatc atgggtgatg aagaattgca aaagcaaatg
    31741 tgccgagctt ctaaagaaca aggaatccct gatgcttcaa aacgtcttta tgacttagta
    31801 aaacaaataa tcaaaaaata aaggcggtgg tcttagtgag taagaaaaaa gaaccaaaaa
    31861 aattgtctca tccgtcttct gatgaaaaca atttgactcc ttggcaaaag gctaaccgaa
    31921 aatatctggc agaacaagag atgaaaaacc aagagccaaa aaaagaagac ataacattag
    31981 atacacccct ttcagattct gaagaagaaa aaacggttca agaggaacag caagaagaaa
    32041 gccagtccga gaaaaaaatc gaattttttg aagagatcaa gcaaaagaaa aacggtggac
    32101 catataacgg ttcttttttg aaccggctgc ccaatttaaa aagtcaacgg cacaaagtac
    32161 tatttcgtcg gttagctttg atcatcactg tattgggtat accgctgatt tttttgatct
    32221 attatgtttc tccttatagc aaattacaag cagttgcagt atcaggcaat aagacagtca
    32281 acagccaaga aatcatctct gatacaaaat tgtcattagg agaaaatgtt tggggacagt
    32341 attttcatcg atctacttat atcgatcgcc ttaaaaaagc acagccaaga atcgaaacag
    32401 caaatataca tttcaaaggg atgaatgaat tcgatttgga tgtaacagaa tataaagaga
    32461 ttgctttgat cgcaaaaaac aatcaatact acccagtgat tgaaaatgga acagtattag
    32521 atgaaaaagt agcaaatcct acaaaaaatc tccctatctt ggaagagttc aaagactctg
    32581 caaaaatcaa agaattaaca aagcaataca atcagttatc atcagaatta caaaaagcaa
    32641 tttctgaaat caagtacacg ccaagagcaa gcaataaaaa tctgatccaa ttgaatatga
    32701 atgacggcaa tcaagttatt gtaaatatca ataatcttgc gaaccaaatg aaatattatt
    32761 cgcaagtagc caaagatatg gatgaaaaag gtgtgatcga catggaagtt ggtatcttct
    32821 cgcatcctta cggtagcact caagaaacgg aagaatcggg ccaatcggaa gaaacaactg
    32881 aaagtgaaga aaatgcggat caaacaaacg aatcaagtga agaggcagaa acgcaaaaag
    32941 cacaaacacc tggatcgtct agcacaaacg gatcatctga ttcaaatgat tcaagtgaaa
    33001 caagtcaaat agaagataat ccaagtacaa ctccgtcaag tttaaatgaa aattgataaa
    33061 acaactttaa aaagtaagaa tgtactttct aaaaaccagt aatttatggt agaattggaa
    33121 gcagtgaata tatctattaa taagtatatt ttaaaacgga cgctagttgt tcgcttgtat
    33181 atggaatagt aagtaggagg gaatcccatt catggcaaaa acaggaatgt atgtaggcct
    33241 tgatattgga acgacatctg ttaaagttgt cgtggctgaa tatatcgaca gccaaatgaa
    33301 tattattgga gtaggaaacg caaaatcaga agggattaac cgaggcatta tcgttgatat
    33361 cgataaaacg gtccaagcga tacaacgagc agtacgacaa gcagaagaaa aagcaggtat
    33421 ccagatcaaa ggcgtaagtg tcggtttgcc agcaaacatg cttgaagtag aaaattgcca
    33481 gggaatgatt gcagttaacg gagattcaaa ggaaatcacc gatgaagatg tcagaaatgt
    33541 ggcttcagct gcccttgttc gttcaatacc tcctgaacgt caaatcgtgt caatattgcc
    33601 acaagatttt actgtcgatg ggttcgaggg aatcaaagat cctcgtggaa tgatcggcgt
    33661 aagattagaa atgtacggtc tgttgtttac aggacctaaa acgatcatcc ataacatacg
    33721 taaatgcgtg gaaaatgccg gattaatcgt caacgaaatg gtgattactc ctttagcgct
    33781 gactgaatca attctttctg acggagaaaa agattttggt acgatcgtca ttgatatggg
    33841 tggcggccaa acaacaacgg ctgttatgca tgataagcaa ttgaagttta ccaatcttga
    33901 ccaagaaggt ggcgaatttg tcactaaaga tatctctatt gtattaaata cgtcatttaa
    33961 caatgcagaa gcgttgaaaa taaactacgg ggatgcatac ccagaacgga catcaccaga
    34021 tgaagaattt ccagtagatg tgattggtca gtcagaacca gtgaaagtcg atgaacgcta
    34081 cttgtcagaa attatttcag ctcgaatgga acagattttc aacaaagcaa aagatgcatt
    34141 ggaccaaatc gaagcattag agttgccagg tggcgtcgta ttgacaggcg gagctgctag
    34201 tcttcctggt gttgtggact tggcacaaga aatcttcggc gtaaatgtaa aattgtatgt
    34261 tccaaatcac atggggctga gaaacccggt atttacaaat gtaataagta tcgtggatta
    34321 ttcagcaaac ttgagtgaag tgtatcaatt ggcaaaaatt gctgttacgg gtgaaacctc
    34381 agcagctcgt caaatggtag ttgaacaaga aacaacaaac acatatgaga gctatgaagc
    34441 acctgaagaa acaatctacg atgagccgga accaaaagag tccggagaaa atgtaaaaaa
    34501 taaaatcaag ggtttcttta caaatatttt tgattaattc atcctgggag gaataagata
    34561 tggaattctc aattgataat aatatcaatg acggcgcagt aatcaaagtc atcggagtcg
    34621 gtggtggcgg tggaaatgct gtaaaccgaa tgattgaaga aaacgttaaa ggtgttgaat
    34681 tcattacagc caacacagac gtgcaagcat taaaaaattc aaaagctgaa acagttatcc
    34741 aattaggccc caaatatact cgtggtttag gtgctggttc tcagccagaa gttggacaaa
    34801 aagcggcaga agaaagtgaa caatccttac gcgaagcttt agacggtgca gatatgattt
    34861 tcatcactgc aggaatgggt ggaggaaccg gtacaggtgc tgcaccaatc gttgcaggta
    34921 tcgcgaaaga attgggcgca ttgactgttg gtgtcgtgac acgtccattc acttttgaag
    34981 gaccgaaacg tggccgtttt gctgcagaag gtatcgctcg tttgaaagaa aatgtggata
    35041 cattattaat tatctcaaat aaccgtttat tagaagttgt agataaaaag acaccgatgc
    35101 ttgaagcttt ccgtgaagca gataatgttt tacgacaagg tgtacaaggt atctctgatt
    35161 tgatcacagc gccaggttac gttaaccttg actttgctga cgtgaaaaca gtgatggaaa
    35221 accaaggtac agcattgatg gggatcggtg tggctagtgg agaagatcgc gtagtagaag
    35281 caacgaaaaa agcaatctcc tctccattgc ttgaaacatc tattgatgga gctgaacaag
    35341 tacttcttaa catcacaggc ggtttggata tgacattgtt cgaagcacaa gatgcttcag
    35401 atatcgtagc aaatgcagca actggcgatg taaatatcat tctaggtaca tctatcaatg
    35461 aagaaatggg cgatgaaatc cgtgtgacgg tcatcgcaac aggtatcgat gaatcaaaaa
    35521 aggaacgcaa gtctagccga ccagctagac aagcacaaat gcaatcacct gctcaaaaga
    35581 ctgtattaga tatggatcaa gctaaaccaa cttcttcaga agaagaaaac agttttggcg
    35641 attgggatat cagacgtgaa caaaatgttc gtcctagagt tgatgattcc aattttgaca
    35701 atatcgaaaa aaaagaattt gatacattca accgcgaaga aacaaaatct catggcgatg
    35761 atgaattaaa tacgccaccg tttttccgtc gtaaaaaata aaggagagga agagcacatg
    35821 attgctgaca acttgcagaa aatcaagcaa gaaattcagg attcgtgtgc tcttgttcat
    35881 cgtgatccga aagaagttac attagtagct gtaacaaaat ctgttgaatc cgctcaagct
    35941 gaagaattgg ttagagctgg cgttatgaat tgtgcagaaa accgagtaga taaattatta
    36001 gaaaagaaac aagatttatt tgattttcct gaaataaagt ggcatttaat tggtaattta
    36061 cagcgcagaa aggtaaaatt gattgtaaat gaaattgatt actttcatgc gttggacagt
    36121 ttaagattag cagaagaaat ccagaagcgg ttagagaaag aattatcctg ttttattgaa
    36181 gtaaatgttt ctggtgagga atcaaaacat ggaatttcac cagatgagct tttaccgttt
    36241 gtggaatctt tagctgcctg cacaaaaatc aaggttgttg ggttgatgac tatggcgcct
    36301 aaagaagctt ctgatgagca aatccataaa gtgtttggta cgctgaaaga attgcgtgat
    36361 cttgttcaag caaaaggtta tgcccatgca ccatgtagtg aattaagtat ggggatgagt
    36421 caagactttc aaattgcgat tgaagaaggt gcaacattta tccgtgtagg aacagcgcta
    36481 ttcaaagacg aataaaggag gaacaaaaat gccgctttta aacagaggag caattgctaa
    36541 ttttttcgga ttagctgatg aggaagagta cgaataccaa gattatcctg aaacaacaaa
    36601 aaaggctgta cagccaacta aaaaaaatac aacacagaat caatcaactc attctgtatc
    36661 tgctgcgaat actcatgaaa aacaaaaaac ggcgacttca cgtcctgtaa cgcatgaaag
    36721 tattcagccg gctgaaaaga aaaaagtaat tgaactacat caatcaccta caaaaactgt
    36781 agcgaaagaa agaacagctt caagaacctc tacacaacct aaagcaaatc ttgctggaaa
    36841 aatcactgta atggaaccaa gagcttattc tgaagctatg gcgattgcta aacgaatcat
    36901 tgctggagaa gcaaccttag tgaattttca tttagtagaa gaaaaacaag cgagaagaat
    36961 cgttgatttt ctaacaggaa cagtttatgc actagatgga gatatcaaac gtgttggaga
    37021 tgagattttt ctttgtacac cgactgggat ggagattgac agttcaaccg ctcaatcttt
    37081 tgcagatgcg aatttgtttg atctgtaaga ggaggaacta cgatttatac tattgcacta
    37141 ttacttcatt tacttcaacg ggcagcctat ttctatagca ttatcttgat ggtatatgca
    37201 ttgctctctt ggtttccagg cgggtatcaa tcagggctag gacgattatt agctaagatc
    37261 tgcgaaccgt atatcagttt gttcgatcgt ctgcctttga ggattggacc cttggatttc
    37321 acaattttag gggcaatcct gatcttgaac ttagccgtac aaggtttagc acagatcgtt
    37381 atatggataa ctgtccattt agtttattga ttgaagcaac aaaatcttta ttctcagaat
    37441 agataggagg ggaaaatttt ggatgcaaat gtataccagc actttcgggc agatgaacgt
    37501 acatttatcg attcggtagg cgattggatt gaacaggttc aaggacaata tgcaccctat
    37561 ctgaccgaat ttcttgaccc aagacaatcc tatattttag aaacactgat tcgacaaagc
    37621 agtgatttgc gtttcatgtt ttatggcgga tatgaacagg cagaaagaaa aagatgtttg
    37681 attttccccg attattacga acctgtagag gaagattttg aaacagcatt gtatgaaatc
    37741 cattatccta gcaaatttgc cagcctaagt catgggaaaa tccttggaac tttagtggga
    37801 acggggatca aacgctctta ctttggcgat atcatctcgg acggtgatcg ctggcaagta
    37861 tttattgcaa atgaggtgga tcattttgtt gctgcacaag taacaaagat tggaaaggtt
    37921 gctgtacgct tggaaccaat caaatacaca gaaatgatta tgccgaaaga tagctggaca
    37981 caagaaagag gaacagttag ttccatgcga ctagacagtg tgatctctga gatttataat
    38041 atttccagac agcggtccaa gcagctgatc gaagctggta aagtaaaagt gaactggaca
    38101 gaaaatacaa gacctgattt tgtacttgaa ctattagata ttatttcagt cagaggttat
    38161 ggcagagtcc agatccaaga acttgagggg aagacgaaaa aagaaaagta cagggtactg
    38221 ttcggtgtat tacgtaaata aaataaaaag gtggtttact catggcttta actccattag
    38281 atatccaaaa taagacattc ccgacaaaaa tgcggggata taatcaagac gaagtagatg
    38341 atttcttaga tttagttgtt cgtgactatg aggaattgac tcaaagaaat cgagaattag
    38401 aaaaagcagt caaacattct gaagaaaaac ttgagtactt caatgaatta aaagatgctt
    38461 tgaaccaatc aatcatcgta gctcaagata cggctgataa agtaaaaaca agtgcaagca
    38521 aggaatccga agtgatcgtt acttctgcac aaaacaaagc agatgagtta gttgcgaacg
    38581 ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga aaaagcaaga aaaatcttga
    38641 cagatgcaac agaaaaagca agacagcttg cgacagaaac tgaagatctt aagaaaaaaa
    38701 cacgagtatt ccatcaacgt atcagcttga tgcttgagtc tcaattagaa caagtgaaaa
    38761 gtccggaatg ggatgaaatt ttacaaccat tctccagcta cgtgactgac agccatgaag
    38821 tgatcaaaga agttttagca aaacagcttg acaatgaaaa tgatactgat gtaaactcag
    38881 acacaggagt catagaagca cctatgacgg catttgatac acaggctatt gatatctcga
    38941 tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc actttgttat tcttaaagcg
    39001 agccgatgag agtggaagtt cggtaagaca ctgcatggtg aaagatcctc tacttgagac
    39061 ctgcttttcc gaaattagag taagaagcag cggctgacct cgttattggt cacagagagt
    39121 ttgcactaga tagatgcaac taaatttggg tggtaccacg gcatgttcgt cccttgcggc
    39181 tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt gtatggttga atgaaaatga
    39241 aagaaacctt gcaattagga aaaacaaaat tccctatgcg tggtaatttg ccaaaccgag
    39301 aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta ccaaaaaaga caagagttga
    39361 acgaaggaaa acctagcttt gttcttcatg atggacctcc atatgccaat ggtaatatcc
    39421 atttaggaca ttcattgaat aaaatcagta aagacattat cattcgttca aaatcaatgt
    39481 ctggctatcg tgcaccatat gttccaggat gggatacaca tggattgccg attgaacaag
    39541 tattggctaa taaaggcgtg aaacgtaaag aaatgtccat ggcagaatac ttggaaaaat
    39601 gtcgtgaata tgcactttcc caagtagata agcaacgagc agatttcaaa cgcctaggag
    39661 ttgcaggaga ttgggaacat ccttatgtta cattagatcc ttcttatgaa gctgcacaag
    39721 tacgcgtatt tggtaaaatg gctgaaaaag gctatattta caaaggattg aaaccaattt
    39781 actggtctcc gtcaagtgag tcttctttag cggaagctga aattgaatat aaagatgtaa
    39841 aatctccatc tatttttgta gctttcaatg tcaaagacgg caaagatatt cttgatgaag
    39901 atacagcttt tgtcatttgg acgacaacac catggacatt gccagcaaac caaggtattg
    39961 ctgtaaatcc aaaatttacg tatgtattgg tggaagccga tggccgtaag tttgtcgtag
    40021 cgaaagattt gctagaaaca gtccaatcag aaatcggttg ggaaaatgta acagttctaa
    40081 aagaatttgc tggacaagaa atggaatata tgactgcaag tcatccattc tatgaacgtg
    40141 aatcacttgt gattttaggc gatcatgtta ctttagacgc tggtactgga ttagtccata
    40201 ctgctccagg acatggggaa gatgactata tcgctggtag caaatataaa ctatctgtag
    40261 tgtcacctgt agactcaaaa ggattgttta cagaagaagc acctggattt gaaggtatct
    40321 tttatgacaa agcaaatcct atgatcactg atctattaaa agaaaaagga gctttgctga
    40381 agctagattt ctttactcat agttatcctc acgattggcg gacgaaaaag cctgtgatct
    40441 atcgggcaac tccacaatgg tttgcatcta tcgataaatt ccgtcagaat attttggatg
    40501 aagtagaaaa agtagagtgg gttatcccat ggggtaaaac acgtttgtat aatatgatcc
    40561 gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg gggagtacca cttccgattt
    40621 tctatgctga aaatggagaa gcaatcatca caccagaaac aatcgaacat gtggcaaaat
    40681 tatttgctga acatggatca attatctggt ttgaaaaaga agcgaaagaa cttcttcctg
    40741 aaggatttac ccacccaggt tctcctaatg gcgaatttac aaaagaaaaa gatatcatgg
    40801 atgtctggtt tgattctggt tcttctcatg aagctgtttt acgtcagcga ccagaattat
    40861 cattcccagc agatatgtat ttagaaggat cagaccaata tcgtggctgg ttcaactcaa
    40921 gtatcacgac tagtgtggct atcaatggtg tagcaccata caaatcagtt atttctcaag
    40981 gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc tttaggaaac atcattgcac
    41041 cagaaaaagt gatcaaacaa tttggtgcag atatccttcg cttatgggta tctagtgtag
    41101 attatgaagc agatgttcgt gtttctatgg atatcttaag tcaagtggca gaagtatatc
    41161 gtaaaatcag aaatacgatg cgtttcttat tagcgaatac agaagatttc gatcctaaaa
    41221 aagatactgt ttcgtacaat gatttacgtt ctgtagacaa atatatgacc gttcgattga
    41281 accaagtaat caaagaaatc cgagaagaag ggtacgacaa atataacttc atgcatattt
    41341 accgtacagt tatgaacttc ttgacggttg atctatcgtc attctacctg gattttgcta
    41401 aagatgtagt ttatattgaa gcagaagatg attatcaacg tcgttgcatg caaactgttt
    41461 tctatcaaac agctgtagct ttgacaaaat tattgactcc tatcattccg catacagcag
    41521 aagaaatctg gtcattcctg aaagaagaag aggagtacgt tcagttaagc gagtttcctg
    41581 gttatgaaga gtttgcaaac caagaagaat tgatggatac ctggactgct ttcatggact
    41641 tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa cagcaaattg attggtaaat
    41701 ctttggaagc aaaactaact gtttatccaa aacaacaagt tcgtgaaatg ttaaaagctt
    41761 tggatgcaga tattgcacaa ttattgatcg tttcaccaga ctactttgaa gttatggcag
    41821 cagatgaaca agtgccagat aacgctatgg tatttgatga tgttgctatt acagtggaaa
    41881 aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg taaagatgta ggagtagatg
    41941 aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt tgaagacttc tatcctgaag
    42001 ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg gggtgtgaca tgttctttgt
    42061 cacaccccct tttgttatca atcgtttaat tttccacgtt cacgatacca gttatctggt
    42121 tgccagatcc attcgaatcc gtctttttca agtagttcga aagctgcttt aggtcccatt
    42181 gatccagctt tgtagttggg aaaatccggt tgtgtttggt cccaagcttt tcgaatgaca
    42241 tccacgatat gccaagattg agcgacttct tcccaatgag agaagttcgt tccatcacca
    42301 ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc tattttcaac catttctgca
    42361 ctatttctaa attccaattt gattggttcc gtttcaaatc cttgccctac ttctttccca
    42421 ttcaaggata aagaaaatcc ttcagttggc tgaatataga tcgttagaac attaggtgga
    42481 agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga atacattaac aggaacttgt
    42541 ttgaatacga tgttgatccg agtccctttt tcagtcaaac gtttgcctgt acgtacatag
    42601 aaaggaacac ctgaccaacg gaagttatcg atagtgaatt tgcctgcgac gaatgtttca
    42661 gtctctgatt gagcatccac tttgtcttct tcgcgatagc ccttgaattg tacaccatcc
    42721 aattcacctt gagcatattg cccgcgaacg aagttttcta acacttcttc ttcgttataa
    42781 atgcggatag ctttcaatgc tttgatcttt tctgtacgga tttctttttc ggaaaatgct
    42841 actggcggtt ccattgcaag taatgctaca acctgtaaaa tatgattttg caccatgtct
    42901 ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt ctacaccgag gttttcgcca
    42961 aaagtaattt ggatattgtc gatatagcga ttgttccatt gagcctcaaa aatattattg
    43021 gcaaaacgga tagctgagat gttttggatc atttcttttc ctaaataatg atcaatcctg
    43081 aaaatatctt gttctgggaa tacttctctg atttgctggt tcaattcaaa agcagactgg
    43141 taatcagaac cgaacggttt ttctatgatc aaacgattga agccattttt tgtcataata
    43201 ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt gaggtgccat agccaaataa
    43261 tagacacggt taccgtttaa ttgatagtgt ccatctaatc ggtcagacaa ttcttttaat
    43321 gtctgataat gcgttgaatc atttacatca tgggattgat agtaaaaatg gcttgcaaag
    43381 gtagttgctt cttcatctga aggattcaat gcacggattg tttctttgac aacttcacgg
    43441 tagtgctcat cgctccaggg acggcgagcg gttccgatta cagcaaaatg ctcgcctaaa
    43501 tttccttttc tgtaaaggcg gaacaaagac gggtacaatt tacgctgggc taggtcacct
    43561 gtaccgccga atatcgtaaa caatacattt ttttcattag acatgtggat acactccttt
    43621 atattttatc gacaaaaatc gtgctggccg ccttaaagct gacggggatt ttctttctat
    43681 cattttggat agtgatcggt ccttcataag ctgcgatgtc aacgatctca tattcttcgt
    43741 tgatattcag gtcaatagtg actaagtaat ccaacaattc tttttcatca agcactcgtg
    43801 cgatacgcac ttttgtaccg acaggatagg actccaatgt ttgtcggcgt tcttcatgga
    43861 caacttcctc ttttttagga atcgatcctc catgaggaca atgttcagga tgattcaaat
    43921 agttttctaa atgatctgca agatgttccg aagtcacatg ttccaatact tcagcatcat
    43981 catggacttc attccagcta tagtctaaat gttcaactaa aaaaacttcc cataagcgat
    44041 gtttgcgaat cagactgcta gctttttgca gtccgcttgc agtcaattgt acaccttgat
    44101 aaggggaatg ttctacaagc tcttctttca caagtttaga aatcatttca ctgacagatg
    44161 cagctgagac atctagacta gatacgattt gtttattgtt gactttcgtt gtatctccac
    44221 ctagttctaa aatgattttc aaataatctt ctcgatttgg tgtcatattc gatcatcctt
    44281 tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa acgttaattg actatttttc
    44341 taaataaaaa acaaaggatt tttagacgtt tttacaacaa aaaacgaaag atgatagaga
    44401 agactcattc tttcgtttat aaaggatata tatatacatt gggagggatc tatttttcag
    44461 ataacaactt atatgaagtg tctgcagatt cgtcataaag agtttgttcg ggataagaaa
    44521 agataagata aaaaccatac tgatcacgta cgactaaacg atattcatct atttctacca
    44581 cagatgtgcc aagagactga ccgttgatca aaaacgtgta atcagatgta atctgtaatt
    44641 gttgtttttg ctggctatca ctaaagaacc atgtaccttg atactttgag agtccttctg
    44701 acttttccgc ttgttttttc tttttcaagt aaataaaact tgagagaacg gtaatgagtg
    44761 aaccaagaaa atagatcatt ttttttatca ccaaatcact cctttatgag aaaataatcg
    44821 catagacata ttctttcttc tttatttcat tgtaccgaat aaaagctaaa atttctctaa
    44881 gactttcgac tggttttttt actgtttttc tacaaattat gataggatac atagtaggaa
    44941 gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag ctttttttat tgaccgcccg
    45001 aatcggttta tagcacattg tatgaaaaaa gacggagaaa taattattgc gcatgtcaaa
    45061 aatacaggta gaggaaaaga ggtcctgata ccaggcgctg aagtggctat tgtatttgct
    45121 cctggaccta aacggaaaac cgcctatgat ctgattgctg taaaaaagaa gggtgactgg
    45181 tttaatatcg atagtcaatt gcctaatcgg ctggcaattg atggtatttt agacggcacg
    45241 atccttttac caggattaac tggtccattt acggtataca agcgtgaagt aacttatctt
    45301 cactccaaat ttgatattta tttagagacg gtggtcggac aaaaagcatt tgttgaagtc
    45361 aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc ctgatgcgcc cacactcaga
    45421 ggattgaaac atataggaga attaacgtat gccgcacaag atggttatgc cgcttatgtc
    45481 ttgtttattg ctcagttcga acatcttcat ctggcaacga tccatgaaga aatgcaacca
    45541 gcactagctg atatggttcg acatgcgcag caaagcggag ttcaaatcct cgcctataat
    45601 tgtcaagtat cggcagatga ggtaactgtc aagcaggcca ttccatttga tgtagatatc
    45661 ccttttgaag atcctataca atagaaagaa ggtaaaataa tgatcaactt aggtattatc
    45721 ggaacaaatt ggattactca tcaatttgtc caagcagctt taagcacaaa aaaatatgaa
    45781 ttgacagcgg tatattctag acatttggaa aaagcacagg aatttggttc tcattatgaa
    45841 ggggatatcg aattcgcaac agatttgaag acttttttca atattactca tatggacacc
    45901 atttatattg cttctccgaa tagtcttcac tttagtcaag ctaagcaagc aattttggca
    45961 ggaaaaaatg tgatagttga aaagccagct ttttcgacgc caaaagaaat ggatgaaatc
    46021 atccatttgg caaatgaaca caatgtgtat ttttttgaag cagcaagaaa tatccatgaa
    46081 tcaggtttta aaaagattgc cgattactta ccattaaaag acgagataat cggtgcaaat
    46141 ttcagctata tgaaatattc ttcgcgctat gaccaagtat tagatggaga agaaccgaat
    46201 atcttctcga ctcatttctc aggtggtgca ttgatggatt taggtgttta ccttgtatat
    46261 gcagctgttg cttggtttgg aatgccaaaa gaagtccatt atttcccaag aaagatttct
    46321 actggtgtag atggtcttgg atgggggatc ttacgttatg agaattttga tgtatcgatc
    46381 cagccaggta aaatcggtga ttcttattta ccgtcagaga tttattttga ctcaggaacg
    46441 ctagtgatga atggagtcaa tgcaattgaa caagcacaat ttcatgatcg cgcgcataaa
    46501 gaaatcattg atttggcggt aacagctgca gaaaatccaa tggcagatga agcagaggat
    46561 tttgccaatg tcatggctca tccgaaagat ccagcttggg gattgttgta tgaagagtgg
    46621 atcgagctag ctagaaatgt gaaccaagtg atttatgatt taagaaaaaa tggcggcatc
    46681 aaattcgatg cagataatga ggaagatttt taatgaacga atttgtacaa gtatttcccg
    46741 aaaattggca gaaaaaatat gaggataaag ggtttatctc accttctctt attcaacgag
    46801 cagtttttca gccactaagt aataaacaaa gtatagtagc ggtttctcct acaggttcag
    46861 gtaaaacact ggcttatctt tggcctctac tactaaacgt ggaaccaggt gaagcaagtg
    46921 cgttggtcat ttttgcttct tctcaagaac tagctattca agtagcagat gttgcacgtg
    46981 aatggggaaa agacaaagag ttgaaagtcc aatctttggt gggcggagcc aacgtcaaac
    47041 gtcagattga gggtttgaag gaaaaaccag aaatcttgat tggtacacca ggaagaatat
    47101 tggaattgat gaaagcaaaa aaaataaaag cacatcaagt gaaaacaatg gtatttgatg
    47161 aagcagacca gctatttgac gctgggaaca gccaaatcat tgatcaaatt cttcatcaag
    47221 ctccaacaga gtatcagcta gctttttttt cagcgacagc agatcgttcc cttgaaagca
    47281 ttgaaaaaat cactgggaaa acgtttgaga caattgacgt gacagcagag gacgattctc
    47341 gaaaaggact gagacattac tttttacgtg taccgatacg aaagaaagat gactacttga
    47401 gacgtctcac gcatatagaa gattttcatg gtctgatttt ctttaatcaa ctgactgaat
    47461 taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc ggtaggaagt ctggcctctg
    47521 atcagaacaa gctactccga aaagcagcgc tagaacaatt caaaaagaaa atggtcagag
    47581 cacttttgac cacagatgtg gcggctagag gtttagatat tacaggtctt ccttatgtag
    47641 tgaatgcaga agttccgtta agtgaagaag cttatttgca tcgttcggga agaactggtc
    47701 gaatgggtaa tgaaggaata gtaattacac tcgtacaaga taataatcta agagatttaa
    47761 aacggctttt acgtccatca aatataacac ttgaagagat ttttttatac ggaggtaagc
    47821 tgcaaactga gcagccaacc aaagaagatg cagctcctgc agtaaataaa aagtatgcga
    47881 aaactttttc tggaaaggag cagaacccag aatataacaa agaaaaaata agaaaaaaag
    47941 ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag gcgcaaataa gcgccttttt
    48001 atgctatgat tatgaaaacg ggaacaaaat tggttgccag aggaggggtt atgtgaaaga
    48061 acgtcaagaa gagatcttgc agctattgac agaaaaccaa caaggattga cagcaagtga
    48121 tgtagctgag cgcttaacta tcgatcgaag caatgcaagc cgatatttaa gtgaactata
    48181 caaggcccat catattgtaa aaaccgctgg ccgaccagtc gtttactctt tgccaacgga
    48241 aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg caagtgactt ttgaaacact
    48301 cgttggagaa aatgattctt taaaagtcag catacagcag gcaaaagctg cgattcttta
    48361 tccaccgcgg gggttgcata cgattatttt tggagaaact ggtacaggga agtcgatgtt
    48421 tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg ctttctgcag atgcgccatt
    48481 tgtttctttc aattgtgcgg attatgctca aaatccgcaa ctgctctttg gacacatctt
    48541 tgggatcaaa aaaggggctt acacaggagc agctcaagat agtcccggtc taatagccaa
    48601 agcagacggc ggtatattgt ttttagatga gatccaccgt ttaccgccgg aagggcagga
    48661 aatgctgttt actttcattg ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt
    48721 acatgaagca tctgttcaga tcattggggc gacgactgaa tcttcagaaa gtttcttatc
    48781 tacgttcaat cgacggattc ctatggcaat caccttgccg aatcttgctt cacgttcact
    48841 ggatgagcgt tatgaaatca tctctctttt tatcaaacaa gaagcgaatc gtttgaatca
    48901 acgtatcgat atcgaaaaag aagcgattct tgccttcatg ctttatgatg cggaaggaaa
    48961 tatcggtcaa gtgaaacgag acttgaaact agtctgtgca aaatcttttt tacattaccg
    49021 tactcatcat gagaaaaagc taatcattcg aaaagaagaa ctttcccttc aagtacaaaa
    49081 aggattactg aaaatcaagg aaataccgga acgtctcgac cgattcatgg acagcaaaag
    49141 tcagtatttg acatttgaac caggatttgc tgatgttgtc tggtcacaag atccagaacg
    49201 aaacatgcag gtctataacg atattgagga aaaagtcact tctctttccg aaaccggtgt
    49261 ggagaacatt gatctagaga ccctcatttc aaaagatgtg gatgcttatt tccagacata
    49321 tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt ttgccagatg aaatctggcg
    49381 gttaaccaat caattgtatg atgtggctga aaaacggtta gaccgtattt ataatgaaaa
    49441 agctcgtttt gcttttgctt tgcatctgca aagtacattg gatcgtgtga gagaaggtca
    49501 tatgatcgtc cacccagatt tgaataacgt acggaaaaat tttaagaggg aattccaggt
    49561 agcaatcgat ctttcaacga tcattgaaga agaagaacaa gttgagattc cttttgatga
    49621 aatcggcttt attagtatgt ttctttctat caatctagga gaaagtgagc ctgttaaaga
    49681 aaacaaagta gatgttctca tattgatgca cggcagacag actgcttcaa gcatgttgga
    49741 gatggcacaa gaactattag gaacaaaagt agggaaagct tttaatatgc cactggttat
    49801 ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc aaaaactatc aagagcattt
    49861 gacgaatggc ctactcttgt taacagatat gggatcatta aacaccttca gtgatttgat
    49921 ttatgaagag actggcattc ggacgaaagc tatcacgatg acaagtacca tgattgtgat
    49981 agaagcgata cggatggcaa gtgttggaag aagcttagaa gatatttatc aaaatatcca
    50041 gctttccttt gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg aaactttaca
    50101 gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa ggagtagctg ccaaattata
    50161 tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt
    50221 agaacgtgaa tcattcaaaa aacacatcga tgccttattg gaagaatatg agatcaaagc
    50281 aattgcggga acagttgatg ttgattatca aaatatccca tttttttcag cctatgacgt
    50341 ttttgatgat gaaaaactaa atattttaaa gcgtatagct gatgatgaga tcccgacagc
    50401 taaaattgtc gattcacttg ctggatcatt aagcgatgtc ttttctattg atgaactgat
    50461 ccgttcattg caaaagacag ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc
    50521 tggagtagat actggaatcg ttatccattt agccttcatg ttagacggat tgatcaaaca
    50581 agaaagccat cggcaatttg agaaactgtc tattttctca aaaatttatc gtttggaaat
    50641 ggatatcgtt cgggcaaact tgatggcact agaacgaaaa tatcaggtaa aaattccaga
    50701 agatgatatc gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca
    50761 ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag
    50821 catgaaaacg ctatttcagc tctttttttt attggcatgc ttcttgcgtt atataaaagt
    50881 gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag
    50941 cagggatgag tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga
    51001 tggaagccga tatttttgcg gtctctgcct cagatgcaga taataattta gaatctaaga
    51061 atgtcgatgt attattatta ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa
    51121 aacttgcgcc taaaggtatt ccattagatg tcatcaatat gcaagactat ggcatgatga
    51181 acggagaaaa agttcttgca caagcagaaa atctaatgaa ataaatcaag tatgcagttg
    51241 ggggtataac aacgtggaag atcaacaaaa tctagaagcc atcatggggt taatcatgtt
    51301 tggcggtaat gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa tcggtgattt
    51361 tgagttagcg gataggaaaa tcgctgatgc agaagaatca cttatacaag cgcatcatgc
    51421 acaaacagac atgttgacac aagaagcaca aggaaatcat atgcaagtga cattattgac
    51481 tgttcacagt caagatcact taatgacatc tattgcgttt actgatttag caaaagaaat
    51541 catcgaactt tatcgtcgcg tatacagcga ataaatacaa cttctttttt gaacggaaga
    51601 agaaaaattt ttgaacggat tttttatttt gttataagga gaactctctt tatataaaaa
    51661 acttatagga ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa atacatcctg
    51721 ccggttgccg caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga tgcttttatc
    51781 ggcatactgc ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt
    51841 gatttaccgc aacagtttaa cagtacctat gctgacagtt tagcagcaga caaaggaatc
    51901 tttgccgtat tgaatgtcat catcggaatc aatggttttg tctggaacgg tacactagca
    51961 atcgccggat tgatctttgc cttttcttgg gggtataatc ttgcccgagc ttatggagta
    52021 aatgatctag ctggggggat cgttggttta gctaccttga ttcaaggtat ttcctttggt
    52081 ccagcattat cagcagcatt agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat
    52141 agttctgaga ttggtgcaac gattgcagat ggcacactgt cagttggatt atggggttgg
    52201 ctaaaactag atcatttgaa tggaaacgct tattttacag ttatgatcat gggagctatt
    52261 tcagtcatta ttttttgtaa attgatgctt gccaacatta cgatcaaaat gcctgattca
    52321 gtaccaccag cagtatctaa agcatttgca gccattttac cagcgacgat cgctttatac
    52381 gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg atgggcaact gatcattgat
    52441 ttggtgcaaa aatatattgc agaaccattc ctagggttat ctcaagggtt gggagcagta
    52501 ttgatcgtca cagtatttgt tcaagtattc tggttctttg gtattcatgg atcaaatgta
    52561 ttggcaccag tgctagaggg aatctgggga caagcacaac taatcaatat tgatattttc
    52621 caaaaaggtt ataacggaaa aactggtaca ccagccgtgt tagatgcaat cgaagatgga
    52681 aaagcttata tgtgggtccg cggatcgttc gatgcttttg catggtttgg cggatcaggc
    52741 ggaacgatcg tcttactgat agctatcttg attttctcta aacgtgcaga ttatctaaca
    52801 gtagctaaat tatccattgg gcctggtatc ttcaatatca acgaaccagt gatgtttggt
    52861 ctgccaatcg tattgaatgc aatcatgttt atcccattct tgattgctcc agtagtagca
    52921 acagcaattg gatggacagc tacttatctt ggcctagtcg ctccggtatc gcagcaagtc
    52981 acatgggtag taccgccgtt cttattgtca ttcttagcaa caggagcgga ttggcgggca
    53041 ccgatcgtaa cattagtatg tatggtcgtg actttcttga tttgggcacc atttgtcatc
    53101 tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg
    53161 gacgctttaa gtttcgttcc tcataatgaa ttttcgagga ggaactgaaa tgattacaat
    53221 tgccagtgca atgcaacaag atgaaatcaa agaattgtta gaaggataca atgaagaagg
    53281 aatgactttt acatttaaag aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga
    53341 agatccggaa gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc cttggggaac
    53401 agtcctttat tttcaatcaa cagctgttta gaaaatggtt taaaaagata ggatggttga
    53461 gatgataaaa aacggtttat ttttatgtag tattggttct ttgatcgtgt tgtatagttt
    53521 gaatccacat tcgcaaaggt atgatttgta tagtatggtc acaggtcttt ttctaacagg
    53581 attaggaatc tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa
    53641 ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt ttttttacga taaagttatg
    53701 gactctgtcc ttttcgatgt acgtaaagcg accggcaaat cagtaactag aaaacagctg
    53761 aaaaatttgg agtacgtcaa acaattctcg caaaataacc gggctcgcat aaaaatcgaa
    53821 gaagtcattg aaaaccagtc ttataagttt cgtacatcga caacgaagaa tgaattcgtc
    53881 gttcattatc aaatcaaacc attggacgat tcgacctgtg aggttcatta cacagaaaaa
    53941 atggagtctt atggcatgct tcaaaagttg aatgatatgc ttttaggaac aatcttgatg
    54001 tattttaaac ggagacgttt caaaaaaatg ctagatatga tcgaagcaac gtattaaatc
    54061 aatgaaacat ggtactattt agttattgaa aaatgaagta agctagaagg aatttttcat
    54121 aggaagatag atctttcctg cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa
    54181 tgacaaacaa agtaagagag ctaggtgtat cttcgattga ggaaatgttt gatttagcca
    54241 cttatgcatt taatggggca gatacacctg aaaggcgaga acgttttcgt acacttgcgg
    54301 agaattcttg gaattacgga ttttttgatg aggaggatcg gctaacaagt caagtcatgg
    54361 cgaccccttt tccagtgaat ttttacggac aagagtacct aatggccggc attggctatg
    54421 ttgcttctta tccagaagca aggggacaag gcgggatcaa tcagatcatg gaaaagatcc
    54481 ttgaagattg ccgtgatcgt aaagtaagct tatcttatct ggcgccattc tcctatccct
    54541 tctatcggcg ctatggctat gaacaatcat ttgataaaat cagttatcaa ctaaacagtc
    54601 gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg
    54661 caaaagctgc aatcagaaga atttatgaac agatgccgga aaaccaacga gctggtctga
    54721 agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa aggcaattac cagtttgctt
    54781 tgcattttga tgataatgag gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt
    54841 cctttattat tgcggagtgg ggatttttgg atcatgatgc tttttgcagc cttgttcgct
    54901 tcgtaagttc tcacaatggt gcttttgaaa catttgaata ttcatgcggc tacggcggta
    54961 atgatcaaaa ttatctatta gaaaatccat ttgcttctgt aatgatcaca ccttatatga
    55021 tggcgaggat cgtagatatt tctttgtttt tggaaaatta tccatttaaa aagagacaag
    55081 ctgcatttgc cctacaaata gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg
    55141 aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa
    55201 tcgttcgagg aagtatccaa catatcacgc agctattatt agggtatcgt aaagtggaag
    55261 agttggtatt ccaagaaaga atacaggtga caaaagaata tgaagaaacg ttaagctcaa
    55321 tcttgccaca gcaacagcca attcttaatg actatttttg ataaaaaacg ggtgatgaca
    55381 atatttttgt caccaccctt ttgtttttca tttggcattt gaaggaaaat gttaaagtaa
    55441 atcctctttt ttcgtaaatt tgaatttttt gttttctttt gttttagggt tttctttttt
    55501 gtggtcttgg attgcttctt tcgtaatctt ttcccgattt tttttagatg aatctaagcg
    55561 ttctgcgtat actttttctt ttggcttatc attcattgga aaggacctcc ctacattatt
    55621 tcattctacc tctagtttat catctgttaa ggactagtgg aaacgttttg cctaacttat
    55681 aagctgattt ttttagcaga taagcttgca agtaaaaaac aaacaagcta caattgattt
    55741 attgaaattg ttcctgtagc tcagtaggat agagcaatcg cctcctaagc gataggtcgc
    55801 tggttcaatt ccagtcagga acgtataaat aacccctgta tcctttgcgg tacaggggtt
    55861 tgttttgtat ttgtgccaaa aatgtgccaa actagttatc atttttatat aaactatcga
    55921 acacagtatt gattttttct ttatcctgat cttcaagctc tcgaacaata tgaagataag
    55981 tagacattgt agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta
    56041 ctccttgata aagtaaaata gatgcatgtg tatgccttag tccatgcaaa gtaaattgct
    56101 tttcaatccc taattctttt aattttttgc gcaaatattt gctgacggcg tttgatgaaa
    56161 ctaatccatt ttttaagttg aaaaaaacaa aattatgagg gtttttgatt tcaaaattct
    56221 cgaataattc tttttgattg attttaaact tttttaagag atcaatcgta tgtttatcaa
    56281 tggaaatttt cctatttgaa gtttcgtttt ttgtttttcc ccattcattt aatttataat
    56341 tccatgtttt attaatagtg atgatttgat cttcgaaatc gatatcttcc catgttaatc
    56401 ctagaagttc agcaaatcgc attccagtgg caccagcaac taacacgagc ataggagaag
    56461 agtactgcgc agaaaggttt tcttctacga gtttcattaa agatttaaat tgatcataat
    56521 ctaaatattt atcttctttt ggcttcaatg aatcttttcc tttgattact gcttttctag
    56581 ttggatcaaa cggaattaat ccttcttcta cggcatcttt caacgacgct ctaatatggt
    56641 tattaaactt aataacggta gattttacat ggtctttagc atacttattt aaaaagcgtt
    56701 gatatcccac tctatctaaa tcagaaatca ataccgctgg catatatttc tttatgttca
    56761 ttaacgtatc ttcgtatttt ctataagtga taggagatac tgttccttct ttataaagtt
    56821 gcatccaatc ctcaaagtaa tctgataata atagattttt gcgatcccct ttaaggcctt
    56881 tgttcaagtt atattctaat tcgttggcgg catcttttgc ttctcctttt gtcttaaatc
    56941 ctgattttct tatcttcgtg tattttccgt catctttttt gtatgaaatt tcgtattgcc
    57001 aactatttcc acgtttgact aatcttgcca taattgaatc agctctcttt ctctgataca
    57061 ataggaacta taaagaagcc cattgtttgg gttttattat tttttgcaca tcctttttgc
    57121 ttgccggcgc aaggatgtgc tatttgtcgt tttaatggta taattggatg tgtattagat
    57181 catccaatca gaggtgttta ttatgattaa agtgattttt aaaagaataa ctgaacttcc
    57241 agagtttaga ttatttgttg attattggta tgtctggttt tttattgttg cattgctgat
    57301 aggtctagat gtcatattag aaagaaaact taagtaagag ttgagttaat aaattgaatt
    57361 cctaaatgag atattttgta gcccatattt tgtttgccga catcacctaa tactatggag
    57421 ctaatcgttt tttctaacag accaaaatca gataatctat ttaagctacc atctattagc
    57481 tcttgtgaat agccagacat gctgatattt ccagtgaaat tttcgtttcc acgactcatt
    57541 aagcatgcta acaattgaat ctcaatcgat gttaattgat tcagtacaag aagataatac
    57601 tcctcgttat cccatgaagc gttgttaatt cgaagtaaac aattcttata aagattttta
    57661 tacatatcta ttttattttg tgcctttgat ttttctacct cgtcatttat cccttcgaat
    57721 attccaatga gttggtcctt gttctctaag ttgaaaacat cttctggaat ttgatcgcga
    57781 atgttttcta atgattcgtt taaatttttg tagaatttct cgatgcgttt gaaacgcttc
    57841 tcgtttttag acccaaaata aagtgactgc acaacgccac caatataagg aattgctcca
    57901 ataccgcctt caactattaa ttcaagatat tcttttttgg ttagtttttc attgctatta
    57961 tccatagtta cctcctattt tgtcatttcc tttacaagca ttgtgactcc tgatggcact
    58021 cttttgtata ggttttatta ttgtgcacaa tctaactctt agtggggcgt ggttcattta
    58081 ttttttactt actttactat taatcatatc ccattgagca taggattttt ctatgttcga
    58141 tcctcttgaa ctaatttctc ctgtaaaagt tactttatcg ccctttgtaa aggtatcagc
    58201 attgtttaag tccttcgcaa aaattacata tgaaacttta ggtgttgcag aaatatcatt
    58261 ccaactttta tcatcgtact ctttgtctgc tataatggct attctagtag tcattgattc
    58321 gataacagtt ccttcaaaag tatatgtttt accactaata tatttagtaa aagctgtatt
    58381 ttgctcagta gctggagtaa tagaataata actatctaag aaagcacccc agtcatgatt
    58441 atttgtctct aaaaattctt caaattgagt taggctttta ttgctgctgt tagatgtagt
    58501 agtttgagta tcagatgatg agctttcctg tttgttattg tcatttgcct cgtttgatga
    58561 ggaaagcgag aaacctacgg tagtaaaaat aatcccgata ataaggagaa tcataggtgt
    58621 tttcttattt gtttttttga taaatcttac aattaacatg attaaaccta taataaataa
    58681 aacaatacca gtaaaaccaa atataccgtc cattaataga tcctctttct cttttgatat
    58741 tgaatattat aaaccaagca attctttttt cttaaaatcg aattcttctt gattaataat
    58801 cccttcatct aacaactctt tatacttttt aatttcatct gcacttgaaa tttgtgtttc
    58861 tttggaatta ttatctaata tagattgttg cttctctata tattttttta aattttccat
    58921 ttcattccaa tatttttttg taaacataac ggtgttttca tctttcgtgg ccgctaatat
    58981 acctccgcga ctttcgtttc cgcctagcaa ggtaaattgt atataaccat tactcatacc
    59041 tggttttttt aattgtacgg ctgatatatt tttaaagggg atagtttttt ctcctttaat
    59101 accttgatta actaaattta taaatccttt tctaataatt gaaatagaat catcagttac
    59161 tttaatcaag gtttttcctg gagattttat taaaatctct ttttccattt tttcttcctc
    59221 atttctgtga tatgattttt tttgtagaag ctcatatatg aggaaagagc ccgtgtt
//
LOCUS       DAZUMI010000002        54935 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000002 DAZUMI010000000
VERSION     DAZUMI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.002"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..874
                     /locus_tag="ACG81C_000067"
     CDS             194..874
                     /locus_tag="ACG81C_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5063266.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            964..1998
                     /locus_tag="ACG81C_000068"
     CDS             964..1998
                     /locus_tag="ACG81C_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5063267.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2012..2347
                     /locus_tag="ACG81C_000069"
     CDS             2012..2347
                     /locus_tag="ACG81C_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063268.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2331..4811
                     /locus_tag="ACG81C_000070"
     CDS             2331..4811
                     /locus_tag="ACG81C_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063269.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            4867..6426
                     /locus_tag="ACG81C_000071"
     CDS             4867..6426
                     /locus_tag="ACG81C_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063270.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6423..7787
                     /locus_tag="ACG81C_000072"
     CDS             6423..7787
                     /locus_tag="ACG81C_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5063271.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7958..9028
                     /locus_tag="ACG81C_000073"
     CDS             7958..9028
                     /locus_tag="ACG81C_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063272.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9043..9711
                     /locus_tag="ACG81C_000074"
     CDS             9043..9711
                     /locus_tag="ACG81C_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063273.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9731..10309
                     /locus_tag="ACG81C_000075"
     CDS             9731..10309
                     /locus_tag="ACG81C_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5063274.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10710..11621
                     /locus_tag="ACG81C_000076"
     CDS             10710..11621
                     /locus_tag="ACG81C_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5063275.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11709..12626)
                     /gene="rnhC"
                     /locus_tag="ACG81C_000077"
     CDS             complement(11709..12626)
                     /gene="rnhC"
                     /locus_tag="ACG81C_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5063276.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12828..13265
                     /gene="zapA"
                     /locus_tag="ACG81C_000078"
     CDS             12828..13265
                     /gene="zapA"
                     /locus_tag="ACG81C_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5063277.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13324..13872
                     /locus_tag="ACG81C_000079"
     CDS             13324..13872
                     /locus_tag="ACG81C_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS5063278.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            13987..16347
                     /locus_tag="ACG81C_000080"
     CDS             13987..16347
                     /locus_tag="ACG81C_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5063279.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16434..16748
                     /gene="trxA"
                     /locus_tag="ACG81C_000081"
     CDS             16434..16748
                     /gene="trxA"
                     /locus_tag="ACG81C_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5063280.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            16930..18708
                     /gene="uvrC"
                     /locus_tag="ACG81C_000082"
     CDS             16930..18708
                     /gene="uvrC"
                     /locus_tag="ACG81C_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5063281.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18853..19752
                     /locus_tag="ACG81C_000083"
     CDS             18853..19752
                     /locus_tag="ACG81C_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063282.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19745..21007
                     /locus_tag="ACG81C_000084"
     CDS             19745..21007
                     /locus_tag="ACG81C_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063283.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21168..23066
                     /locus_tag="ACG81C_000085"
     CDS             21168..23066
                     /locus_tag="ACG81C_000085"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5063284.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23396..24115)
                     /locus_tag="ACG81C_000086"
     CDS             complement(23396..24115)
                     /locus_tag="ACG81C_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5063285.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24305..24352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24452..24784
                     /locus_tag="ACG81C_000087"
     CDS             24452..24784
                     /locus_tag="ACG81C_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063286.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24771..26774
                     /locus_tag="ACG81C_000088"
     CDS             24771..26774
                     /locus_tag="ACG81C_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5063287.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26793..27263
                     /locus_tag="ACG81C_000089"
     CDS             26793..27263
                     /locus_tag="ACG81C_000089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5063288.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27284..27868
                     /locus_tag="ACG81C_000090"
     CDS             27284..27868
                     /locus_tag="ACG81C_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS5063289.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27883..28869
                     /locus_tag="ACG81C_000091"
     CDS             27883..28869
                     /locus_tag="ACG81C_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5063290.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            28859..29170
                     /locus_tag="ACG81C_000092"
     CDS             28859..29170
                     /locus_tag="ACG81C_000092"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5063291.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29192..30973
                     /locus_tag="ACG81C_000093"
     CDS             29192..30973
                     /locus_tag="ACG81C_000093"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5063292.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            30966..32342
                     /locus_tag="ACG81C_000094"
     CDS             30966..32342
                     /locus_tag="ACG81C_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5063293.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32349..32984
                     /locus_tag="ACG81C_000095"
     CDS             32349..32984
                     /locus_tag="ACG81C_000095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5063294.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33014..34369
                     /locus_tag="ACG81C_000096"
     CDS             33014..34369
                     /locus_tag="ACG81C_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5063295.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34561..35955)
                     /locus_tag="ACG81C_000097"
     CDS             complement(34561..35955)
                     /locus_tag="ACG81C_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5063296.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36047..37018
                     /locus_tag="ACG81C_000098"
     CDS             36047..37018
                     /locus_tag="ACG81C_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5063297.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37015..37797
                     /locus_tag="ACG81C_000099"
     CDS             37015..37797
                     /locus_tag="ACG81C_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS5063298.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(37966..38337)
                     /locus_tag="ACG81C_000100"
     CDS             complement(37966..38337)
                     /locus_tag="ACG81C_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5063299.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38471..38914
                     /locus_tag="ACG81C_000101"
     CDS             38471..38914
                     /locus_tag="ACG81C_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5063300.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(38957..39766)
                     /gene="yidA"
                     /locus_tag="ACG81C_000102"
     CDS             complement(38957..39766)
                     /gene="yidA"
                     /locus_tag="ACG81C_000102"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5063301.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39785..41152)
                     /locus_tag="ACG81C_000103"
     CDS             complement(39785..41152)
                     /locus_tag="ACG81C_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5063302.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41136..41966)
                     /locus_tag="ACG81C_000104"
     CDS             complement(41136..41966)
                     /locus_tag="ACG81C_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5063303.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42129..42566
                     /locus_tag="ACG81C_000105"
     CDS             42129..42566
                     /locus_tag="ACG81C_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5063304.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42642..43262
                     /gene="rpoE"
                     /locus_tag="ACG81C_000106"
     CDS             42642..43262
                     /gene="rpoE"
                     /locus_tag="ACG81C_000106"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5063305.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43451..43957
                     /locus_tag="ACG81C_000107"
     CDS             43451..43957
                     /locus_tag="ACG81C_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5063306.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            43974..44873
                     /locus_tag="ACG81C_000108"
     CDS             43974..44873
                     /locus_tag="ACG81C_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5063307.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            44960..45844
                     /locus_tag="ACG81C_000109"
     CDS             44960..45844
                     /locus_tag="ACG81C_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5063308.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46036..46107)
                     /locus_tag="ACG81C_000110"
     tRNA            complement(46036..46107)
                     /locus_tag="ACG81C_000110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46072..46074),aa:Arg,seq:ccg)
     gene            46167..47501
                     /gene="rpoN"
                     /locus_tag="ACG81C_000111"
     CDS             46167..47501
                     /gene="rpoN"
                     /locus_tag="ACG81C_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5063309.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47650..48741
                     /locus_tag="ACG81C_000112"
     CDS             47650..48741
                     /locus_tag="ACG81C_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5063310.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            48782..49783
                     /gene="gap"
                     /locus_tag="ACG81C_000113"
     CDS             48782..49783
                     /gene="gap"
                     /locus_tag="ACG81C_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5063311.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            49893..51083
                     /gene="pgk"
                     /locus_tag="ACG81C_000114"
     CDS             49893..51083
                     /gene="pgk"
                     /locus_tag="ACG81C_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5063312.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51187..51942
                     /gene="tpiA"
                     /locus_tag="ACG81C_000115"
     CDS             51187..51942
                     /gene="tpiA"
                     /locus_tag="ACG81C_000115"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5063313.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52058..53356
                     /gene="eno"
                     /locus_tag="ACG81C_000116"
     CDS             52058..53356
                     /gene="eno"
                     /locus_tag="ACG81C_000116"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5063314.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            53755..54894
                     /locus_tag="ACG81C_000117"
     CDS             53755..54894
                     /locus_tag="ACG81C_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5063315.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        18257 a         9576 c        11607 g        15495 t
ORIGIN      
        1 aacgaaaaaa taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg
       61 taaacgattt tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg
      121 gatgaattca tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa
      181 ggagtgattt attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct
      241 aggcggcttg gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac
      301 ctttactgaa ccaacaaata ccgtggagcc tcttaaccct actgatcctt caaaaccagt
      361 agagccagca gatccggaaa atccagcaac tggccagaca ggtcctttaa cactggatgt
      421 agttcccgaa ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt
      481 agacgcttca aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc
      541 agatggccaa gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc
      601 acaagttttg caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga
      661 taacgaatcc actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc
      721 gacaccaata tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga
      781 tccagcaaat atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt
      841 gacttggaac ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc
      901 ggtgaaaata ctataggtga aggatttttt accgcagttt ttgttgaaag gatgtggtgg
      961 acaatgaatc aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc
     1021 atttttccgt caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta
     1081 tttccagaga accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa
     1141 caaaatcaga cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag
     1201 gtctctgtca atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac
     1261 ccaacgttag atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag
     1321 gaaatcaatg tatcagcaca tgcagaaaca actgtaccta tagaagtgaa aatcccagca
     1381 aaaagcttta agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa
     1441 acagaaaatg ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc
     1501 gttttacaag aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg
     1561 gacgaggtca acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt
     1621 atcagcaatt tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat
     1681 acgtctaatg catttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct
     1741 ggtgataaag caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt
     1801 aatgaatggc gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat
     1861 gaaaattccg tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt
     1921 gctgcagttc tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa
     1981 aaaggagaaa caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat
     2041 tattgcaagt ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc
     2101 aggtacgatt acattttatg atctaagtcc aattccgcat ccaagtggtt cagtcaatgg
     2161 cgcatcaaaa cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt
     2221 cccggagacg aatgaacaaa agatccgttt tctttatttg atcattggta tagaattatg
     2281 gcttcttgcg tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac
     2341 aaaataaatt ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat
     2401 ttatcttttc accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc
     2461 catacacgta tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga
     2521 agcttgtcca actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg
     2581 atggttcaaa tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga
     2641 aactaacaaa tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga
     2701 tcatcaattt gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca
     2761 acgcctactt gtatattgcc ggtgccccca tcaaggttac tagcggaagt ttattatcag
     2821 caggaagcca taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattgacag
     2881 atattattgc tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg
     2941 atgttacagc taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa
     3001 acaaaatttt gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg
     3061 ttgattttta tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc
     3121 cagctgcagg tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg
     3181 cattttctgc aagtgcagag ataaatacgt ggaatagtta catctcacca agtgattcaa
     3241 caggaaaata taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc
     3301 ttccaggctc taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc
     3361 agttgccatt tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac
     3421 aaggagcaat actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata
     3481 tcgtgtacag tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat
     3541 tggagcagta caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt
     3601 ggggaaagct cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac
     3661 taaaaaaggg ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag
     3721 gatggacaga tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta
     3781 cttctacact acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg
     3841 atgtacttac taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa
     3901 tcaccactct tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc
     3961 taaaaaacct tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata
     4021 cgattcaaga aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa
     4081 aaatcacgat ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg
     4141 gtacaactat cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga
     4201 gcaaaatagg agataccatt catcttactt acaactatca attaaatact gtagatactt
     4261 ctttacaatc cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag
     4321 cagatggtgc taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc
     4381 ccaaacaaga attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc
     4441 ccaaaccgtt gaagacaagt tattatgaat caaaaggcga tttttctttt gatgtgcggg
     4501 atactcggct tccaagcacg tctccttggc aactgacagg tacattgact tctttattta
     4561 agaatgatca aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa
     4621 agcaattgat tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag
     4681 gagaggttct cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta
     4741 cgaatgctca acctggcgca acgtatcaag gcatggtcac atggaaactg acagctgggc
     4801 caaccagcta agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt
     4861 tcaaggatga gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg agtggacttg
     4921 gaacttttca gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta
     4981 ttagaggaaa aaggagaggt ttccgttaat aacgtactta cccgcttatc agttgatcgg
     5041 gctaccttga aagaagatct gttttattta caagctaact tggaaaatat caaacaattg
     5101 gaaattgtta tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc
     5161 gatgtttatt atcattgtta cttgcgctat tctacaaaat atcagatact tctttatctg
     5221 ttggaaaatg gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct
     5281 actttgtctc gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata
     5341 aaaagcgggc ggctgaccgg tccagaaagt caaattcgtt atttttattt ccaattattt
     5401 tggtttggac gtccctatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact
     5461 tttttagatc tgctgcaaaa agagtttagt tttgctatta cagaagaagg acgattgaaa
     5521 tttgaaatat ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca
     5581 gattatagca atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat
     5641 acatttttga cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg
     5701 gttttttatc ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag
     5761 gagtttttga taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt
     5821 aaacaatcga tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag
     5881 attgcgttga cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat
     5941 atttttggcc aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg
     6001 gttttatgtg aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac
     6061 gaggaaaatg cccgctttga attatttgct cgctatgcat ctattttacg gacgctgatc
     6121 aagcagttag aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg
     6181 gatatcgtga aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc
     6241 gaatacgaga aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg
     6301 tcacagaagt ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc
     6361 tatttgaacc aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa
     6421 acatgaaaat cgtgatcatt ggtgcttccc atgcaggcat cacagctgcc ctaaatctaa
     6481 gaaaacttca accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag
     6541 gctatgtttc aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag
     6601 ttgcccataa catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag
     6661 taactgaact tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg
     6721 agaccataac ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc accttgtata
     6781 aacaaattga agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac
     6841 ttgccgcttt aaaagaagca aaagaagtag tgatcttcgg tgctggttac attggattgg
     6901 aattagcaga tgctttgaga aataaaggat acattattca tctagttgat tatatgccga
     6961 atgttttatc aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa
     7021 ctaagcaaat aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga
     7081 aagtagtatc cgtacaactt ctgtctcaaa caataaaggc agatatggtc attttttctg
     7141 cccaaactcg tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga
     7201 ctgtgatagt caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata
     7261 tcgtcccagt cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag
     7321 ctgtacatat ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc
     7381 tcagacaaaa gataacagca gcagtcatta tagactactt tttaggaact gtagggctga
     7441 cagaagatga agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc
     7501 tatttccaca atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg
     7561 atacattaga aataattggc ggacagctga taagccaaga attcttatta tcagatttga
     7621 acctgcttgc ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt
     7681 ttggatttct gtctgaatac acctctcgtt ttcactacct caatgagtta gcttttaaag
     7741 tactagctaa gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt
     7801 cttttattgt gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat
     7861 gaaatagcgg aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat
     7921 tgttgtataa aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat
     7981 tatgcattca aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct
     8041 ggtatttttt ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga
     8101 caagtgggag gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc
     8161 catgattact aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca
     8221 aacacctgga gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt
     8281 cttcggtatc gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga
     8341 taacgatgat gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccatagg
     8401 agatacagtt aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca
     8461 tgccaaattc aatgtctcac cagttctcta tacaaccatc aatgcttacc aagaaatccg
     8521 cttcgaaaaa gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa
     8581 aatcagtaat ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt
     8641 acctggatat aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat
     8701 tgcggctatt gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt
     8761 tggtgtaatg aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca
     8821 aacattcatt ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc
     8881 ccttgttcta cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag
     8941 cttgttgatg ttagtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa
     9001 aatcgatccg ttgaaggcaa ttggataaga aggaggaaga acatgaaagc aatagaattt
     9061 aaagaagtag aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca
     9121 accatgagta tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa
     9181 agtacttttt tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc
     9241 aatgatcaag aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa
     9301 attggcttta ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta
     9361 ttattagtag ataagattaa acatgaacat cgtcaaaaag aagcaaacga attatttgag
     9421 cagcttggcg tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg
     9481 caaagggtcg caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa
     9541 ccaactgcta gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa
     9601 tcaaaagaca aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa
     9661 tgcgatagaa tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc
     9721 aggtggaaaa atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc
     9781 gactgacttg tttatgtcca agggatatct cggcacttca acaagagaaa tcgcacaaaa
     9841 agttgggatc acccagccca atctctatca ttatttcggt gataaagaaa aactatatac
     9901 ggcagtttta gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac
     9961 aagcgaaacg ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca
    10021 tctggtcgat ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag
    10081 agatcatctt ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc
    10141 agcaaaccaa tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt
    10201 cttagtgtta gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt
    10261 ggcgcaattg atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat
    10321 agggagaaaa tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgcttct
    10381 ttatataggt agtttttcat aaagacaaat ttgttattct ctcatgatta tcagggtata
    10441 tttttattta ttatgtcggt gttccaggaa gtgttttaat ttaataacga agaaattaat
    10501 tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag
    10561 gagatttcag gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt tattttctac
    10621 ggttatatct tttacagccc cttttttgct tacttaagct gattttaagt tttggccctt
    10681 agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat aattggagct tttgtatttg
    10741 caatcattat cattattatc tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg
    10801 gacaaacggt cagcagtacg aacagtacgg ttacaaagac aaaggaatca tttgatgata
    10861 cagaacaaga gacattaatc aaaatcggaa aaaatgaccg tacactatat acagatgact
    10921 cgctaactac cccacttgca actataaacg gcggagagct ggcggagttt ctttctgaaa
    10981 caaaaaatgc ttatcagatc aagacaaatg atggctatac tggatattta gccctcgaag
    11041 atggaaccaa agtcacaaag aatatacaaa caaaaccgga agagctatct gatgcagtga
    11101 ttgtattaga tcctgggcat ggcggaaatg atactggggc attaagcaat gatgaacaaa
    11161 cagaagaaaa agatataaca ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg
    11221 ctggagcaac ggtttatttg acccatacta cagatgaatt agtacagctt ggagatatat
    11281 gcgactacag tgaagaaaaa aaggcagatg tctttatcag tttacacgca gactctacgg
    11341 aatatgcgaa tgaagcaact ggaatcacga catattatta ctatggacaa gaggaaacat
    11401 tagcacagac aattgctgac tccttcactg atctgcctct ggattcaaga ggaatctcca
    11461 ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc tattttaatc gagatgggtt
    11521 atatgaataa tgatagtgat ttaaaggaat tagtaacaga cagttatcag cagcagattg
    11581 ctgcaagttt gacacaagca ttgaccactt attttcagta ataaataagc ccagccaaac
    11641 aaaaatagct cctcatattt ttcgtttggc cgggcttatt atcgcagatg caagcttttg
    11701 aacaccgttt atttttttag tagcttcaaa gctttttctg tatttgcaaa gtgaagtttg
    11761 gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca
    11821 gatttcgttc cagctcctga tggaagggtg attccagctt cagctgatgc tttgtgtaac
    11881 tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt
    11941 tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat
    12001 tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc
    12061 tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg
    12121 gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag
    12181 gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt
    12241 gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca
    12301 catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc taaaacggat
    12361 aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc
    12421 tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt
    12481 ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga
    12541 atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt
    12601 gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac
    12661 cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt
    12721 atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc
    12781 atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa
    12841 aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga cgggaaacca
    12901 agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat
    12961 tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg
    13021 atcaattgaa gaaacaagaa agaatcttgg agctggaaga agaaacagca gaattaaaga
    13081 aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga
    13141 acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg
    13201 tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta
    13261 attaagatcc ttttatcaaa ataagaaaaa tttaaaaaaa gttagaagag aaaggaacgt
    13321 agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt
    13381 cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc
    13441 cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc
    13501 acgccaacat caaagttagc attttttgat caggtatttg cctttcacct agatgaagct
    13561 ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt
    13621 gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt
    13681 gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta
    13741 cttgcctttg ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgc
    13801 tttatcgttg agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat
    13861 attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagattat
    13921 ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt
    13981 gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc
    14041 agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagc
    14101 gaggaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt
    14161 ctgcgaggcg ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatt
    14221 gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg
    14281 acaagcgaat tgaatcgttt cattgatgat ctttatgaca gtgaaatcga atttgcacgt
    14341 ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa
    14401 gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga
    14461 cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg
    14521 aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc
    14581 ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca
    14641 ggacagactt tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa
    14701 taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt
    14761 gttccccatc gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg
    14821 aatgcaaaag cacgatttgg taaagaagta aaagctatcg tgccgggaat cagtgaagac
    14881 aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct
    14941 aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt
    15001 gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta
    15061 ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata
    15121 ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact
    15181 gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca
    15241 ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa
    15301 aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatataat
    15361 cgggcaaata cgattaatgc cagcatggag tttgatgtgg atacattaag tccaacgtat
    15421 cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt
    15481 cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta
    15541 aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa
    15601 atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc
    15661 tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc
    15721 gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag
    15781 cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca
    15841 aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa
    15901 aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt
    15961 ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc
    16021 gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct
    16081 gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac
    16141 gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct
    16201 caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat
    16261 cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat
    16321 ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc
    16381 caagtataga aatgctatac tagtcataga ttttaaaaat ggaggtcttt gatatgtcac
    16441 aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt
    16501 tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac
    16561 aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa
    16621 ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag
    16681 tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat
    16741 atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc
    16801 ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt
    16861 gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg
    16921 gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc
    16981 tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta
    17041 aaaaatcgtg tccgctcata ttttactggt agccataata cgaaaacgga acggcttgtt
    17101 agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta
    17161 gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa
    17221 acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaag
    17281 gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag
    17341 acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag
    17401 gagccttgtc tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat
    17461 ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa
    17521 aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa
    17581 aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa
    17641 atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga taaaggatgg
    17701 atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg
    17761 tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag
    17821 gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt
    17881 gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta
    17941 gttgaattgg cgagtaagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc
    18001 agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg
    18061 accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct
    18121 gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata
    18181 aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat
    18241 tcgcgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa
    18301 ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt
    18361 gctggcatgg caaaaaacga taaacataaa acaaatgagt tgctattcgg tacagatttg
    18421 gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat
    18481 gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct
    18541 tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag
    18601 ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg
    18661 ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact
    18721 ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt
    18781 gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga
    18841 aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct
    18901 gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac
    18961 ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga
    19021 gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt
    19081 ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca
    19141 gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa
    19201 tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa
    19261 aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca
    19321 ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa
    19381 aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc
    19441 gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt
    19501 cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta
    19561 gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc
    19621 ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt
    19681 cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa
    19741 atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat
    19801 ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg
    19861 aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca
    19921 aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc
    19981 tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc
    20041 aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac
    20101 acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag cgagtcaatt
    20161 aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa
    20221 agtcaatttt gattcaaaag gaaaaatgac agtcggagaa gataactcga cagtccaata
    20281 cgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat
    20341 cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag
    20401 tacgactgca caaaagcatt tttacggaaa attaacaggt gttttacttg ttgcattaac
    20461 gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga
    20521 aatggtcaaa aatatattag acggtatctc attggataac ttgattggac cattcttgtg
    20581 gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg
    20641 tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct
    20701 atctctaggc ggatacatgc ttggattgat actaggagct tcagatccaa acaatatcgt
    20761 tattcggatc acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc
    20821 aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac
    20881 attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tttatagcga
    20941 aggcggatta tggagttcat tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa
    21001 agcataaaac aatcctaaaa tacagaagga agctggacat ctattgttcc cagcttcttt
    21061 ttgtatagac ctattttatt tattttacta tattggatat atattcattg aaaatagtgt
    21121 ataacaactt tataatattg agtataccaa tttttggagg cgttttaatg gaccgttatt
    21181 tagatttact ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa atcatcaatc
    21241 tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagtgat atacatggcg
    21301 aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca
    21361 aagacctttt ttctgaacaa atgacggaaa cggatatcag ccaattcact atttttacgg
    21421 cttatccaga atatgcatta acgactgatt ggtacaaaga acaagataaa tcgcagctga
    21481 tccatcattt gatcgatctt ttacggttta cgacagtcaa atatacccgg tcaaaagttc
    21541 gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgacc
    21601 gtatcaatgg aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg
    21661 aagaagaaaa atttttaacg aaacttgcac agacgatcca aaaatctgtc atcgatcatt
    21721 tgcatatcgt tggagatatt tttgaccgag gaactcaagc agccaaagtg atggatcgaa
    21781 tcatggattt ttcttctgta gatatccaat ggggaaatca tgatattttg tggattggtg
    21841 cgtattgcgg ctctgaggca tgtttactga cacttttacg gattgcagca cgctataatt
    21901 atttatttga attagagaaa gaatatgggt tgaatttacg tccattgttt tcttttgctc
    21961 atcaaactta tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa
    22021 gagagcaaga agaattagaa aaaatccatc aggcactttc tatccttcaa ttcaaatcag
    22081 aacatcagtt attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa
    22141 aaatcgacta tgaagcatca acgattgatt tagaaggtca gctctatgag ttgaaaggaa
    22201 cttgttttca aacgatccag ccagatgatc caaaaaaatt cttggaagag gaacaaaaag
    22261 tgattgatag tttgatgtat tcattccagc actctttgag aatgcaaaaa catatgacgt
    22321 ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtgctg tttcatggct
    22381 gtatacctgt agacgagaag ggacagctct tagcatttga attagaccaa tcttatcgcg
    22441 gaaaagaact gctgggcttt tttgaaaaac agataactga aagcgcaaaa gatctgactg
    22501 caaaagaaga tttagcaacc gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt
    22561 tattcggcaa gagtaaaatg gcgacttttg aacgttattt tctcacagag cctcatacgc
    22621 acgtcgagaa aagcaatcct tattatcact tgcgagatga agagtggttc agtgaaaaaa
    22681 ttttagcaga attcgacaca aaagaagaag ggtcagcaat cattaatggg cacacgccag
    22741 taaaagtaaa aaaaggggag tcaccgatca aagcaaatgg aaaagtattt gtcattgacg
    22801 gaggtttatc caaagcttat caaaaaacga caggaattgc cggttattca ctattatgca
    22861 attcttatgg tttccaaatc gtgacacatt atccgttttt gcctattgaa caacaatttg
    22921 caaaaaaatc cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac
    22981 tcaatcgtga tacaacaaaa ggtatggaac tgcagcagca gattacccag ctcaaaaggc
    23041 tgttggacta tcgaaaggac gattgagaag aattccagtg aacattgttc catagactgg
    23101 agactgagac aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt tcttctgcca
    23161 actcctcggt cacaagtcac actattcaac aaacatgaaa agctgttttt tgaaattgct
    23221 ccttgtgact cagagttgaa cggcaggctc acgacctctt ggtataaacg gtgttcaaaa
    23281 gccagtttct cggtattaag ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa
    23341 tttttcttaa tactcgaaac taaacagctt tttcacaacc ccctcagaaa gtctattaaa
    23401 attcattcac aaagaaatcg aataagcgct gttcagatgg atgtacacgg tgggtaagtt
    23461 ccggatgcca ttgaacaccg aggattttat gcatatcatc tttgctttga accgcttcaa
    23521 tgagtccatc gttagaaaaa gcgatggctt ttaataaagg tgaaagatct ttgactgcct
    23581 gatgatggta agaattcaca aaatatggat cggggagaag gttgcttaac aaactgtctg
    23641 aaacaatctt gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta
    23701 ctgaccactt tggatataat gaaagatctt gataaagtgt accttccaaa acgacattca
    23761 atagttggag tccccggcag actgcaaaaa ttggttttct ttgtttcaat gcttccaaga
    23821 tcagtgcctg ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt ggatgaggat
    23881 cttccatgta aagttgtgga ctgacatctt gaccacctgc aagaataagt ttgtcgattt
    23941 gtccaatcag ttgaggagct gaatctggag acatgactgg cagaataaga ggcaatcctt
    24001 gagcatctaa aactgcatcc acgaatcctt gcggtgtata gctcacttga tttccttgga
    24061 aagtatctgt tgctcgaatc agttgatttc cggcaatgcc gatgattggt ttcatttgta
    24121 ttccccctaa agtagactta ttgtaacaag gatttaaaaa aaattaaact atgtaagact
    24181 tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa
    24241 attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta agcagaaata
    24301 aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag gacattttat
    24361 tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat gaaaaaagaa
    24421 agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag ctttggaaca
    24481 aatccgaaaa gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa
    24541 gatctatgca acagaaaaag agcaggaatt ggaaacgatc caaacaaact atcgacaaca
    24601 actaaaacag ttggtggaag aaaaagaaca atttgaaata caagaattag aaaaagaaaa
    24661 aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa
    24721 gcatcagagt caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt atggccgtca
    24781 ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg attttacaag
    24841 cagtacaagg aatccaaaac gttgaaatcc gggatctgta tcaaaataga acaaacaacc
    24901 agtgggtgga gaagtatttt ccagatacca cgattattga taaagaagca aaattaagcg
    24961 ctttaggtaa tcggttgaaa gacatccaag aagctatcca attcatacaa caccacggcg
    25021 atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa gatttggaag
    25081 ccaattattc cgaagaaaat ttcataaata aattgaaaga agtccttgat ttaaaagaaa
    25141 agtgggaaca actaactgaa gaacgtgatc gtttagaaga agaggaagaa tggcttcttc
    25201 attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc atttttgaga
    25261 tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactgagt aagcttgatg
    25321 acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg tatgttgctt
    25381 taaaaaaatc agttgctgtt atagaagaaa ttgctgggaa atacgggttc aataaagaaa
    25441 cgtatcctta tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag caattatcca
    25501 ttgttcacga gcagcaaaag aagataacga cagcgttagg tcagtgcagt ggctatatcc
    25561 aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgagaa aaaataaaag
    25621 aacggtttat ccatgcgact tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa
    25681 aacagaattt gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt
    25741 tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg aaaaaccatc
    25801 cgcttgtcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa tatgaagaag
    25861 tagatccaac accttggatg acaccatttt atcttgtctt cttcgggatg atggtcgcag
    25921 atgttggtta tggtttgctg atgctcattg ggacaatact agctcaaaaa ctacttgttc
    25981 taccacgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcgata ccatcgatca
    26041 tctggggatt gatctacagt tcatttttcg gacagtcatt accaaaagaa ctgtttggca
    26101 tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt ttgattttat
    26161 ccgtgatttt cggtctgata cagattttgg ttggattatt tgtggcagca aaggaaaata
    26221 tcaggagaaa agattacttg agtgctgtca gtgatggatt tgcctggcaa gggatcctaa
    26281 ttgggatcgt tattgcgttg gttggtgcca tgttgataaa aaatacagca tttgtttacc
    26341 ttggcgctgg gctatcgatc attgctgcct tatgtatctt gatcgttccg attatccaaa
    26401 cgccgtctaa agtcaaaggt gctgccaaag gtgcgtataa tctttacgga ctgaccggct
    26461 atatcggtga tttagtcagt tatacacgtt tgatggcatt gggtatttca ggcggaagta
    26521 ttggagcggc attcaatatg ctagttgctt tcatgccgcc agctgctcga ttcagtgtag
    26581 ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg ctaagtgcct
    26641 atgtccatgg cgcacgattg cagtatgtcg aattttttgg gaagttttat acaggaggcg
    26701 gacgtgcctt tgatccgctg aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa
    26761 aaaacaataa ataattggag gatttttata taatgatgga ttacttaatt agtcaaaatg
    26821 gtggaatggt atttgcggta ttagcgatgg caacagcgac gattttttca gggatcggtt
    26881 cagctaaagg tgttggaatg acaggtgagg cagcagcagc attgacaaca agtcaacccg
    26941 aaaaattcgg gcaggctctg attctgcaat tgcttccagg tacgcaaggt ttgtatggat
    27001 tcgttattgc ctttttgatt tttatcaact taggaaatga catgtcagtt gtacaaggac
    27061 tgaactttct aggtgcttct ctgccgattg cttttacagg cttgttttca ggtattgccc
    27121 aaggaaaagt cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa
    27181 aaggaatcat ttttgctgcg atggttgaaa catacgcgat tttaggtttc gttatttcct
    27241 tcctattagt gttgaatgcc taagcataaa ggaggaaaag cagatgaacg ccattgaaaa
    27301 aatcatctct cagatgaacg aagcagcaga gcaggaaaga gcggcgttag aacaagaaga
    27361 gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caagttgaaa cagaacatca
    27421 aaaacaaaaa gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg
    27481 ccagcaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt taagacgatt
    27541 gtttgcagat gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat
    27601 caaaaatgca ttgtattcat tacctttgac aggaaaagta gcattcattg ctggagaaaa
    27661 atcagcagcc tatctttctc agacgttgtt ggatgaatgg aacaatgaac ttccctttat
    27721 gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa acgatcaagg
    27781 agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa cgatgagttt
    27841 tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata ccacgaacta
    27901 aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt tgaacggatg
    27961 attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat ttacagtcca
    28021 tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga gcagagccgt
    28081 ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat ctatacgatg
    28141 cgttatactt tccataatct gaaggttttg acaaaagctg agatgacaaa tcaaaatctg
    28201 gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga tgccattcat
    28261 acgcaagttt caaatgaact tcctgaaagc attatggaca gtatacgaga agtacgtgat
    28321 tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg acagtttttg
    28381 acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac agaagtcatt
    28441 gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca gaaaagaaca
    28501 tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga agtatttttg
    28561 aactttgtag ataaagatat ggaagattac acgaattttc tattagagac agattatgct
    28621 gattttctgc gccctgtcat ccgtgaggga aatattgatc tcgtaaagct tgaacagttg
    28681 aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt cggaccactg
    28741 ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg tttactggtt
    28801 atcggcaaaa gaagccattt cgaatcagaa acaatcagaa agcgggtgag acaagtctat
    28861 gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca aattgtttgg
    28921 ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg aagaaatggc
    28981 aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga gcccagaaac
    29041 gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca atcatcaagg
    29101 atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa
    29161 tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca taaaagtctc
    29221 cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca tgtgtctagt
    29281 gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg cgtctattca
    29341 agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc
    29401 tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa tccaacggcc
    29461 attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag ttcaattgcc
    29521 cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg gagaaacggt
    29581 gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac ataaaatcat
    29641 ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat tcactattga
    29701 tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa
    29761 atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg cacccatgat
    29821 cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag cagcagctgt
    29881 acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa aatgggccga
    29941 tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga cggatgtttt
    30001 aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt
    30061 attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta tctatacggg
    30121 aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca tggcagactc
    30181 cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag aaatgcctgg
    30241 tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg aacgatctgg
    30301 aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt
    30361 atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc gggtcgtaaa
    30421 agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt cgatcgattg
    30481 gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc atatccttca
    30541 agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag aacagcagct
    30601 gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc gtctgacatt
    30661 agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg atgatgtcga
    30721 tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga cctttggtga
    30781 agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg acggtaccgt
    30841 atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat tagaaaaact
    30901 agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat
    30961 gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg atggcggttg
    31021 aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga
    31081 ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa atattcgaag
    31141 gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat cctttagaac
    31201 taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga ccgaaagata
    31261 acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta atcaatccag
    31321 tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc gatcatttga
    31381 atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt cctcataaag
    31441 aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat gatttcgccg
    31501 tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg gaagattttc
    31561 gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa
    31621 tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt gcttatgaaa
    31681 agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag gcattgcgag
    31741 agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg tatctttaca
    31801 cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa ggatcggtta
    31861 ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc cctgacttga
    31921 ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag agtgggattc
    31981 agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga actggacccg
    32041 gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc tatgcacaag
    32101 gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct gatgtagaca
    32161 agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa ggattctaca
    32221 cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg atgttgccaa
    32281 gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca gaagggaagt
    32341 gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact cgattgaaaa
    32401 aacagttgac aactgccaca agagggcata aattattaaa agataagcaa gatgaactga
    32461 tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa atggaagagt
    32521 ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg gaacctttca
    32581 tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta gacaaaaata
    32641 tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg acagacacac
    32701 ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc gatcggttta
    32761 ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt caactgatgg
    32821 cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg acgatccctc
    32881 aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag cgcgcagaag
    32941 taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta tcttttttta
    33001 aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc cggctgttca
    33061 gcttctcgca ttaggatttt ttctcctgat tttagttggc ggcggcctac ttaccctacc
    33121 attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca ctgcaacttc
    33181 tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga acagtgctgg
    33241 tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga tggttccgat
    33301 tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta tattaaaaga
    33361 agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca tcctaaaatt
    33421 ggcctttgtg atccaattga ttggtacggt tgctttatct tttgtatttg tgcctgaatt
    33481 tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt tttgtaatgc
    33541 agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt atttgatcat
    33601 ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc gtgatttact
    33661 ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa ccgttacagg
    33721 taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct
    33781 agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg tgacacctag
    33841 aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc tgattttaac
    33901 gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat tgaaaacaac
    33961 tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta cgagagcgga
    34021 agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt tattttttgt
    34081 tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa cgatacctaa
    34141 aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta cagtaggtct
    34201 tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta tagcgcttat
    34261 gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa aagcacaaag
    34321 aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat ttcatcttct
    34381 gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt gcttcttgga
    34441 tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt gaacctcggg
    34501 tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt tatcaatagg
    34561 ttataattca tattttttta ttttttcttt tacgatactt agtccagaag ctaaaatttc
    34621 aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgtac gtgtagtaaa
    34681 gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa ttatcttagg
    34741 gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg ttggtttttc
    34801 tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta atttagtttc
    34861 taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca catctaatcc
    34921 agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga catgtactgg
    34981 aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc cagctaagta
    35041 gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt gatagtaagt
    35101 tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt caatcacttc
    35161 ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat tacaagaagg
    35221 cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata tatcaggtcg
    35281 cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact gagccaattc
    35341 aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag agaaaagtgc
    35401 agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag attctaagga
    35461 cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc
    35521 tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag ttgattgaat
    35581 aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa aatcaatgat
    35641 atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg gagaaataaa
    35701 tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt tataagcctt
    35761 gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg agggtaactg
    35821 agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa tcaacgatcg
    35881 ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt aattgtcata
    35941 agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt ttaccattga
    36001 ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga aaattattaa
    36061 ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg
    36121 tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg aaattttacc
    36181 tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg agaacagata
    36241 ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt taccttctca
    36301 tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat tgataaacgc
    36361 aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag attacttagc
    36421 aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat tgtcgcattt
    36481 gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata ttttagaaga
    36541 aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa cgtttataaa
    36601 tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt tgacctctag
    36661 taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca atgctgtggg
    36721 cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt ctcaaatcta
    36781 tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag ccatggcaga
    36841 aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt ctgagaaaaa
    36901 aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc tagctattga
    36961 agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt taaaatgaac
    37021 ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg
    37081 gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac
    37141 aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct tagatggttt
    37201 acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc atttgtttta
    37261 tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag cggtaatcta
    37321 tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat gccgaaaaaa
    37381 gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga agcctactta
    37441 gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt gcagccagat
    37501 tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc agaaagtgtg
    37561 caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta
    37621 tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa
    37681 ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat
    37741 cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt
    37801 ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct acaatggttg
    37861 ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat tttaattatt
    37921 ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac
    37981 ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa
    38041 tccttttaag tcagtaatct caagaccagc agctttatgt tgctcgtgta atttttctag
    38101 atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg caccatgatc
    38161 atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat aaacaagtgt
    38221 aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata
    38281 aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca ttttcataga
    38341 ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa atagttgatt
    38401 tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac tgttaaggtg
    38461 gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg attatcaaac
    38521 ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa tcgaggctcc
    38581 aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga aagaagaagc
    38641 gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg
    38701 agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata atccaggata
    38761 tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat
    38821 ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc
    38881 agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc
    38941 tcagtttttt tatttattac agtacatatt ttttgattgc ttcagctaca ccgtgctcgt
    39001 cgtttgatga agtgatgaca tttgctgctt ctttgacaag agaaacggca tttcccattg
    39061 ctactcctag accagcatat tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca
    39121 tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac
    39181 ttgcttcttt atttagtact tcgaagtaat agggcgcact tttgaccgtt gtgtatttat
    39241 cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca
    39301 tcatcatttt gacgatgttc atgtctggtg tcatttcttc aggtgttcga tattttagcg
    39361 gcatcttgac taaacttgct tcgtggatcg tataagcgct gatatctcga ttaggcgtgt
    39421 agatcgcttt gtcatcgatc gaatggagat gactgccgac acggcgtgcc atcacttcga
    39481 tctctaggaa atcttcatgt gtcagtccgt gacgggcgat gattttacct gatttcgttt
    39541 gttgtacgag tgccccgtta taagtaataa cgtaatcatt atcttgaaaa aggttcaact
    39601 cgtttaactg ttcctggaca cctggtaagg gacggccagt gcataaaacg atcgaaacac
    39661 cttttgcaac agcagcggca atcgcttctt ttacttcttt tgttacttct cttttctcat
    39721 ttaataaagt accatcgatg tcgatagcga ctagtttgat agacataatt tcctccaata
    39781 tattttaatt tatttcaatc aatgcgccat tacggatatg gctggcaaat tcttgataag
    39841 tttcgtcaaa taaatcataa tgatcacgca aggacggatc aaccatttct tttggaaaga
    39901 aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg
    39961 ataattctat caaagttccg tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt
    40021 gttctgggcg ataaaaatca taaggtagat cgtagctaga attcacagca gtgtaataaa
    40081 caggattata tccgaccttt tctactaaca aagtgagttc ttggactaat tttgaatccc
    40141 ggttgccaga aaaagtagca gatttcagtg gtttacgatt caaaaaacgt ttagctaaat
    40201 catttaaaat aggatcagca gattcagacc attgagtaaa gtaagtgccc aagaccccat
    40261 catcaagttt gaggtattct tctaaagtaa agcttccgtc aagaaatggc agaagtaatt
    40321 gagaatggag ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca
    40381 gatgctccaa aattacttcc ataccacggg aaacaggatg gaagtacact tgcacataca
    40441 tttgatatcg gctaacgata taatcttcca ctgcatgcat gccactcatg gaaaaagcga
    40501 tcccgccttc ataaggacga atcacacgta atattcgggt aagatcaaat gttccatatt
    40561 ctgttccagt gaaataagca tcccgaagca aatagtccat tcgatctgca tcgatctgac
    40621 tagaaatcat ttggacaact tgtgggttgg ggtaagtttt ttggatcaca ctagctacct
    40681 tttcaggaaa accttcttct actcggttaa gaatctgata cacttctgtt tctggagaag
    40741 tgatgatctc tacagtgatg gcttcatgat tcgtatggaa gatatgttca aatgtatgag
    40801 agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc agccgttcct
    40861 gatcatccca gcctccgttg cctatttttt cttttgaaaa atttcgagaa aagatatcgc
    40921 agattcgtct tgaaatctca taaacgccta atgaatgagt aaaacggcta tgttctgctc
    40981 cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt
    41041 tcgaattgat caaatctaag attacttgat gctggacatg gatatagtta tggactggat
    41101 cacgaaaaac tttttccata ggcagcattt gatatttata aggggttgtc ataatgtagc
    41161 tcctttatgt tattacgttg ttccattctc tggatttggt tgttccattg ctccagctgg
    41221 atttcttttt ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata cagcttttca
    41281 aaagcttttg caaatgcttc tttgacttct tttacaaaaa ggggttggtc aagcagttct
    41341 tcaagtgttg ccattgtagc aggatccact gcaggatagc catttgtacc aaagtcttct
    41401 tttaaagcag attgataaaa tcgcttcatg acttgcccgc gtttttcctg attaccgttg
    41461 acacttaagt acatcataac agaaatccca tttttgatcc gacgctgagc gataccggca
    41521 aattttttgc catggatgct taaatcataa gttccaggac aataagaatg ggtgatttcg
    41581 aatgcttgta tcggtgcctt tggaaaagcg tatttagtca ataggagcat ttgctcgtaa
    41641 gcgtcatcta tggttatttt ttttgtgtca ctttggggga aaatcaaaga aatattcaaa
    41701 attccttcat ctgatacgat ccctaatcca cctgaatttc tgatcactgg agcgtaacct
    41761 gcatctttca gtgtttggat cccgtcactt agaaaaggag ttcgaacatc cttcatacct
    41821 aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa
    41881 tattccgtta atatatctgt taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc
    41941 tgatctagtc gaatcgcagg gttcattcaa ccggctcctt tgtataaatt catattttct
    42001 acaatattat actctaaaat caaagagttg ttttggaaaa aaggttttct gtaaaaatgg
    42061 acaagttggt tgacaaaaca aaagaaacta ggaacaatgt agctatgaga agtaaaggag
    42121 aatgaataat ggaattatca caaggaacac cgatttcaat caaattgcgt acaaaagtaa
    42181 aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg gtgacaattg
    42241 gcgatacgct gtacatacgt tataaagaga ttcaggagga gacgggcgat gagattcctg
    42301 taacgatcaa attagttcca gatggtcatg tacagctgat ccgtgcagga gaaatgcgga
    42361 tgcgtttgaa atttggctat aaagaacggt tagaaacatc ctatcgaaca ccatacggta
    42421 tgatccagat tgctactttt acgaaagaac tgcatgttag tttgaaagac cgtcccacag
    42481 cagggaaggt tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt
    42541 atttgacctt ggattttaca gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat
    42601 tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg
    42661 aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc ttagaacaac
    42721 gtggcgatgt catggatttt tctgatttag ctaaccagat ccaaaactat cttgggaaat
    42781 cagacagtga aattcgtgat tcactcgcac aattttatac tgacttgaat attgacggca
    42841 gctttatctc attaggggat aatcgttggg gcttgcgttc atggtatgct atcgattcga
    42901 tcgatgaaga agtaaaccat ggaatggacg aagaggatga agatactcca cgtcgccgta
    42961 aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat tacaatgacg
    43021 atgacccaga agatacagat ctgacagaag aagatgacga tgatctgttt gatgacgatg
    43081 atgatgaaga tgaagaaatc gctgcttata attctgactt gcaagaaatc ggagcagatg
    43141 acccgacaga tgatgaagaa gacttgccag gaaatatcga agaagattta agtatcatag
    43201 acgaagacga tgatgatgaa gattatgaag aggaagaatt ttctgactct gaaaaagaat
    43261 aaacattatt ttcttacgag aggttgtgac agagacgatc agcctatcag aataaggagg
    43321 tgtgacacga tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata
    43381 aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa
    43441 ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca
    43501 cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta
    43561 cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa
    43621 ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat
    43681 ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat
    43741 tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt
    43801 agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg
    43861 tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta
    43921 ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt
    43981 cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag
    44041 ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa
    44101 agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa
    44161 caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag
    44221 gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata
    44281 ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa
    44341 atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa
    44401 attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc
    44461 ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa
    44521 gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag
    44581 atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca
    44641 aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa
    44701 aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt
    44761 tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc
    44821 ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa
    44881 gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa
    44941 aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc
    45001 cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca
    45061 aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa
    45121 aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt
    45181 acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt
    45241 caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct
    45301 gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc
    45361 gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac
    45421 atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa
    45481 agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta
    45541 tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt
    45601 tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac
    45661 agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt
    45721 tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt
    45781 attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca
    45841 gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg aacacataac
    45901 taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa
    45961 ttttctttgg cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct
    46021 aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt
    46081 acgtgatatc cactacacta aggacgcaag cactgggata attgtaacta aacagataaa
    46141 aaattacaag gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc
    46201 agcagacaca gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat
    46261 ataacagtga ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg
    46321 aagttgttga gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag
    46381 gagaagagac aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat
    46441 tgatcgaaca agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc
    46501 ttgtagaata catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag
    46561 agattggagc tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac
    46621 cagctggagt aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg
    46681 attatgcgcc ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc
    46741 gaaaatggaa agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat
    46801 tcgactattt acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat
    46861 tgtacattat tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct
    46921 caaaccgaaa aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac
    46981 aacaagcaga taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt
    47041 tgaagaaaac gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt
    47101 cccggcaaaa agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg
    47161 aagttgcaac tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat
    47221 atttggaaac agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta
    47281 cgaatagtac agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg
    47341 aattagtcga tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc
    47401 tgaaaaaaga tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag
    47461 gaatcccgag ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa
    47521 aagtatttca ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta
    47581 cagtcgtgag gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt
    47641 ttatcgtata tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc
    47701 gctatgctgg atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg
    47761 caagaacgct ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt
    47821 ttatcggaaa gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa
    47881 aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa
    47941 gtttatcaaa gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa
    48001 cagttggcag aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag
    48061 cagaccaaag tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt
    48121 ccagaaggcg agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa
    48181 caattgtcta atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg
    48241 attggtgaag cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca
    48301 aacggtaacc accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct
    48361 ttactcaatg agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt
    48421 attcatagta ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa
    48481 ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa
    48541 aaaggtgaag tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa
    48601 gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat
    48661 atgaaaaatg caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt
    48721 ttaaaagggg taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta
    48781 aatgacagtt aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg
    48841 tatccaagat gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat
    48901 gttggcacac ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt
    48961 tcatgaaggt tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc
    49021 agaagaatta ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt
    49081 cacttctaaa tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc
    49141 tgctcctggc ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac
    49201 tggtaaagaa acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc
    49261 taaaacattg aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta
    49321 cacaggtgac caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg
    49381 cgctgctgct gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt
    49441 aattcctgaa ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg
    49501 ttcattaaca gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc
    49561 agcaatgaaa gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc
    49621 agatatcgtt ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac
    49681 agttggcgac aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac
    49741 tgcacaatta gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa
    49801 gtgtaaatta tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt
    49861 tttgataata agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg
    49921 atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg
    49981 tgatcactga cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac
    50041 aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag
    50101 aaggaaaatc tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa
    50161 cattcgtacc tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg
    50221 gcgacgttgt cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca
    50281 aaaatgatac agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg
    50341 ctttcggtac agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc
    50401 caacagttgc tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag
    50461 aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg
    50521 taatcgaaaa cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca
    50581 cattctacga agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg
    50641 aactagcaaa agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg
    50701 tctgtgcacc agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg
    50761 atggtttgat ggctttagac atcggcccag catcagtaaa attatttgct gatacattac
    50821 aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg
    50881 ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca
    50941 tcggtggcgg agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac
    51001 acatctcaac aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac
    51061 tagcagcaat caacgataaa taaacgacca agtttgctca attctgacga gtaatcgaac
    51121 aaaaggtgaa gggagttttc atgactccct agccattttt caataaatta aggagtgtgt
    51181 tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa
    51241 gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca
    51301 gtcatcggat ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa
    51361 ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact
    51421 tcaccagcag ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt
    51481 cgtgaatatt tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca
    51541 aatggtatga caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa
    51601 actgctgaat ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacag
    51661 gtgtcaaaca tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct
    51721 gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat
    51781 ggaaaagaag tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat
    51841 attgctgaat acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg
    51901 caagcagatt cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa
    51961 aaatgcaaga aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat
    52021 cccttaggaa aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta
    52081 cgctcgcgag atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga
    52141 aagcggagct tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc
    52201 agttgaatta cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt
    52261 cgacaacgta aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat
    52321 ggctatcgac aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc
    52381 gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt
    52441 acctttatac cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa
    52501 catcatcaac ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat
    52561 gcctgttggc gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc
    52621 attagcttca atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt
    52681 tgctccaaac cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa
    52741 agctggctat gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt
    52801 ctacgacaaa gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac
    52861 tgacgaaatg atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga
    52921 agacggatta gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga
    52981 caaagttcaa ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg
    53041 tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac
    53101 agaaactttc gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca
    53161 ccgttctggt gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg
    53221 ccaaatcaaa actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact
    53281 acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa
    53341 cttaaaaaac aaataattct tttctaatga agatacatga tcgactattg aaagatagtc
    53401 gagtatgatc acttagttaa gtaatcgaac tctctacttg ctgaaatatc agcaggtaga
    53461 gagttttttt gcaactagct tctgtatatg atgtttacaa agaatggtac aagaaaagat
    53521 catccttgaa aaaggaaagc taacaataac tagcatcata taagagcttt taaggataga
    53581 acagtagaat aggaatctat gagcaagtcc tctcgtataa ataaaatagg agggaagtag
    53641 cagcatattt ttcataaagt ggctctcttt cttcattttt actttggttg ctcctatttg
    53701 aaaattcgag cacactcaaa tacaataaaa agaaatggca agggagagga ttagatgaaa
    53761 aaagaagaaa aaatcgcgat tttacaagag atcatccgaa ttaaatcagt gaatggaaac
    53821 gaaggtgaag tagcagccta tttgaataag cttttggcaa agcatgacat aacaggagag
    53881 attgtttctt acagagatgg tagagataat ttgattgctc gttaccaaaa gggccaatca
    53941 ggaaaagtgt taggcctatc tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct
    54001 tggacctatg caccctttgc agcagaaatc catgggaacc gtttgtacgg acgtggtgct
    54061 acagatatga agagtggctt ggcagcaatg gtgattgcca tgatcgaact gaaagagtca
    54121 ggaaaaccat ttaacggaac agtcaaatta ttagccactg tcggagaaga agtgggtgaa
    54181 ctaggtgggg aacagctcac gaaagcaggc tatgtagatg acttagatgc cttgatcatc
    54241 ggagagccaa ccaattacag tttgatgtat acgcacatgg gatcaatcaa ctatacagtc
    54301 acctcgcacg gaaaagaagc gcatagctct atgccagacc aaggttataa tgcaatcaat
    54361 cacctaaatg aattcattac gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc
    54421 gagaatcctg tcctaggaaa aacaatccat aatgttacat tgatcagtgg cgggaatcaa
    54481 gtcaacagca tcccaagcca tgcgcagctc caagggaaca ttcgatcgat tccagaatac
    54541 ccaaatgata aaatcattgc attgctgcaa tcaatcgtca atgaattgaa tcaagagaca
    54601 gactatcatt tggaattgat gatcgattac aacaaaatac ctgtcaaagc tgatccagat
    54661 tctcctttga ttcattgcat tcagcaacaa ttcagccagc cattaccttt agttggagcg
    54721 gcagcaacaa cggatgcagc cgaatttacg aaagctaacc attccttcga ctttgtcgtc
    54781 tttgggcctg gtgtcgttac tttgcctcat caagtagatg agtatgttga aatcgataat
    54841 tatttggata tgatcgaaaa atatcaagga attattttat cctacttagc gtaagatttt
    54901 tttaataaat gaaaaaacag agccagatta gcatt
//
LOCUS       DAZUMI010000003        51536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000003 DAZUMI010000000
VERSION     DAZUMI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.003"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..268
                     /locus_tag="ACG81C_000118"
     CDS             95..268
                     /locus_tag="ACG81C_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063316.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(334..942)
                     /locus_tag="ACG81C_000119"
     CDS             complement(334..942)
                     /locus_tag="ACG81C_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS5063317.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG81C_000120"
     CDS             complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG81C_000120"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS5063318.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(1969..2940)
                     /locus_tag="ACG81C_000121"
     CDS             complement(1969..2940)
                     /locus_tag="ACG81C_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS5063319.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG81C_000122"
     CDS             complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG81C_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS5063320.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFGTDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG81C_000123"
     CDS             complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG81C_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5063321.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG81C_000124"
     CDS             complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG81C_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS5063322.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG81C_000125"
     CDS             complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG81C_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS5063323.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(7382..7549)
                     /locus_tag="ACG81C_000126"
     CDS             complement(7382..7549)
                     /locus_tag="ACG81C_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063324.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(7707..7892)
                     /locus_tag="ACG81C_000127"
     CDS             complement(7707..7892)
                     /locus_tag="ACG81C_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5063325.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            8037..8408
                     /locus_tag="ACG81C_000128"
     CDS             8037..8408
                     /locus_tag="ACG81C_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5063326.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            8666..8995
                     /gene="mobC"
                     /locus_tag="ACG81C_000129"
     CDS             8666..8995
                     /gene="mobC"
                     /locus_tag="ACG81C_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS5063327.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            8956..10284
                     /locus_tag="ACG81C_000130"
     CDS             8956..10284
                     /locus_tag="ACG81C_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5063328.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            10281..10652
                     /locus_tag="ACG81C_000131"
     CDS             10281..10652
                     /locus_tag="ACG81C_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS5063329.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            10835..11071
                     /locus_tag="ACG81C_000132"
     CDS             10835..11071
                     /locus_tag="ACG81C_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063330.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(11371..11574)
                     /locus_tag="ACG81C_000133"
     CDS             complement(11371..11574)
                     /locus_tag="ACG81C_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS5063331.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(11578..12522)
                     /locus_tag="ACG81C_000134"
     CDS             complement(11578..12522)
                     /locus_tag="ACG81C_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063332.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(12523..16443)
                     /locus_tag="ACG81C_000135"
     CDS             complement(12523..16443)
                     /locus_tag="ACG81C_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS5063333.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(16592..18673)
                     /locus_tag="ACG81C_000136"
     CDS             complement(16592..18673)
                     /locus_tag="ACG81C_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS5063334.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(18670..19899)
                     /locus_tag="ACG81C_000137"
     CDS             complement(18670..19899)
                     /locus_tag="ACG81C_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS5063335.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(19889..20140)
                     /locus_tag="ACG81C_000138"
     CDS             complement(19889..20140)
                     /locus_tag="ACG81C_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS5063336.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(20163..22154)
                     /locus_tag="ACG81C_000139"
     CDS             complement(20163..22154)
                     /locus_tag="ACG81C_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS5063337.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(22186..24585)
                     /locus_tag="ACG81C_000140"
     CDS             complement(22186..24585)
                     /locus_tag="ACG81C_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS5063338.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(24515..24937)
                     /locus_tag="ACG81C_000141"
     CDS             complement(24515..24937)
                     /locus_tag="ACG81C_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS5063339.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(24951..25487)
                     /locus_tag="ACG81C_000142"
     CDS             complement(24951..25487)
                     /locus_tag="ACG81C_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS5063340.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(25524..26387)
                     /locus_tag="ACG81C_000143"
     CDS             complement(25524..26387)
                     /locus_tag="ACG81C_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS5063341.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(26479..26694)
                     /locus_tag="ACG81C_000144"
     CDS             complement(26479..26694)
                     /locus_tag="ACG81C_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS5063342.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(26771..28579)
                     /locus_tag="ACG81C_000145"
     CDS             complement(26771..28579)
                     /locus_tag="ACG81C_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5063343.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(28576..29052)
                     /locus_tag="ACG81C_000146"
     CDS             complement(28576..29052)
                     /locus_tag="ACG81C_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS5063344.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(29127..29339)
                     /locus_tag="ACG81C_000147"
     CDS             complement(29127..29339)
                     /locus_tag="ACG81C_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5063345.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(29361..30326)
                     /locus_tag="ACG81C_000148"
     CDS             complement(29361..30326)
                     /locus_tag="ACG81C_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS5063346.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(30817..31338)
                     /locus_tag="ACG81C_000149"
                     /pseudo
     CDS             complement(30817..31338)
                     /locus_tag="ACG81C_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(31354..32052)
                     /locus_tag="ACG81C_000150"
     CDS             complement(31354..32052)
                     /locus_tag="ACG81C_000150"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5063347.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(32009..33670)
                     /locus_tag="ACG81C_000151"
     CDS             complement(32009..33670)
                     /locus_tag="ACG81C_000151"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5063348.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            33888..35288
                     /locus_tag="ACG81C_000152"
     CDS             33888..35288
                     /locus_tag="ACG81C_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5063349.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(35540..36619)
                     /locus_tag="ACG81C_000153"
     CDS             complement(35540..36619)
                     /locus_tag="ACG81C_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5063350.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(36705..37664)
                     /locus_tag="ACG81C_000154"
     CDS             complement(36705..37664)
                     /locus_tag="ACG81C_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5063351.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(37676..39187)
                     /locus_tag="ACG81C_000155"
     CDS             complement(37676..39187)
                     /locus_tag="ACG81C_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5063352.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(39209..39772)
                     /locus_tag="ACG81C_000156"
     CDS             complement(39209..39772)
                     /locus_tag="ACG81C_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5063353.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(39778..40641)
                     /locus_tag="ACG81C_000157"
     CDS             complement(39778..40641)
                     /locus_tag="ACG81C_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5063354.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(40655..41551)
                     /locus_tag="ACG81C_000158"
     CDS             complement(40655..41551)
                     /locus_tag="ACG81C_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5063355.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(41726..43015)
                     /locus_tag="ACG81C_000159"
     CDS             complement(41726..43015)
                     /locus_tag="ACG81C_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5063356.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(42987..44504)
                     /locus_tag="ACG81C_000160"
     CDS             complement(42987..44504)
                     /locus_tag="ACG81C_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5063357.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            44702..45592
                     /locus_tag="ACG81C_000161"
     CDS             44702..45592
                     /locus_tag="ACG81C_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5063358.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG81C_000162"
     CDS             complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG81C_000162"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5063359.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(46501..47973)
                     /locus_tag="ACG81C_000163"
     CDS             complement(46501..47973)
                     /locus_tag="ACG81C_000163"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5063360.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(48610..48972)
                     /locus_tag="ACG81C_000164"
     CDS             complement(48610..48972)
                     /locus_tag="ACG81C_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5063361.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            49195..49947
                     /locus_tag="ACG81C_000165"
     CDS             49195..49947
                     /locus_tag="ACG81C_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5063362.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(50087..51010)
                     /locus_tag="ACG81C_000166"
     CDS             complement(50087..51010)
                     /locus_tag="ACG81C_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5063363.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(51025..51297)
                     /locus_tag="ACG81C_000167"
     CDS             complement(51025..51297)
                     /locus_tag="ACG81C_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063364.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        12835 a        12718 c        11439 g        14544 t
ORIGIN      
        1 gaggggcaag gctttttttg agtggtggaa gtggattaga gcatggcaat agcaaaacag
       61 catagcggca tcctcctatg ggccgccaca ccttttgctg ccagaaaaga acggcggcct
      121 tgatttctca aaaccgccgc cagcaaaata caaaatgggc gtgggaaagg tctgcccaac
      181 agcggttttt ttcttttctg ccgctgggca gattgaatac tataatttgc aatgtattac
      241 aaaagatgct ttaaccactt tccagtaaaa gattgtccat gaccagcata gattttatca
      301 aatccaaaat ctgatacaaa accaatactt tcattatgaa acggcaaaat taaaataggt
      361 atcggggtat ggctcatcaa tcaacttata gtgccaccat tcaaagcgat aagactgaaa
      421 tccgctgctt tccatgattt tacgcaagca tcttcgattt tgtgcctctg catcccctat
      481 cccttttgct gtatgatgcg accgttcgtc cataaaatcg aagttacttc ccattgaaac
      541 aagttcccct gtatccaggt ggtagagcgt aagatcaatt gcgcttccac ggctatggct
      601 ggattgtgag gccacataac cctttgtaat caactcggct cgctctatat tggggtaaaa
      661 tttttctttt gtgagattgt cctccggttg cgctgcccaa cgcaggaagc agtccaccgc
      721 agttcttggc cgatatccat cccataaaag caagccatag ccaagcgcag ccgcatggat
      781 ttgtgcctcg cgcagagcaa acgccacggc ctttgtgccg atgatgcgat tcacctcata
      841 cccatccacc ggttttcctg tgaagttatc ccatgtggcg tacttggcat cccaacggac
      901 gccatgcaac atttcatcta aaaacaaaaa accattttcc atacttatca cctctttaac
      961 gccaatgtaa tcaggctgtc aatcagtgca ggaagcgtga ttcctgcggc ggccatcata
     1021 cgtgggtagc ggctgtacga tgtaaaaccg ggcagggtat tgacctcatt tagaacgatg
     1081 ccgccatcct cctgcaaaaa aagatcaaca cgggcaagcc ctctgcatcc aagcacccga
     1141 tatactttct ttgccgtttc ctgcacccga tttcgttcct cgaccggaat gtctgcggga
     1201 actgtaatca tcgcattttc tgagcctttt tccggctcgt tttcctgatg gatgcggaag
     1261 gtaccgtggc tcagccggat ttgatccact tcgccgacaa tcaaatcatc ctcgttcccc
     1321 atgaccgcac acccgacctc acagcccgaa atcgcttgct caattaagat ttttccatca
     1381 tattgtcctg ccgcttctat cgcagcgtta agttcttccg taccgtttac tttggttacg
     1441 ccaaaggacg aacctgaccg tgccggcttc acaaagacag ggtaggtaag cgcacccgcc
     1501 tccggcttgt cacctttatc aatcatttga aattcgggaa cggcgatgcc cgcatttttt
     1561 gtaagaatgt aggccagtga tttgtccatg caagctgcgg agctttgaat atcacagccc
     1621 acatagggga taccagacaa tacaaacagc ccctgtatcg caccatcctc cccgcatttg
     1681 ccatgcaaaa ccgggaaagc cacatcaata cgccgtgttt cgtattcgct ttctttcatg
     1741 acaagcagcc catgcgtttt cctatccggg gagagtatgg cggggagact gtcggcttcc
     1801 cattccgtac atggcttctt gcatagcttc catacaccgt tttttgtaat tccgatgtag
     1861 tgcggatcga atttttccgt atcaatgttc gcagcaattt ctattgcgga ttttaccgac
     1921 acatcatgtt cctccgagca accgccgaag atgattgcga cttttattct attcatgctg
     1981 taaactcctt tcaaaattga gacaattcct gattgttttt tctgtagtat cctgcagcac
     2041 acgctcagtg tagtacgccg tatggggtgt gatgatcaca ttcggcatcc ttaaaagctg
     2101 cgacaaaaag ggatggtcaa gcactttctg cgagcaatcg gtataaacaa actgatcctc
     2161 gccctccaac acatccagtg ccgcaccacc cagctttccg ctttccaacg cctccaccag
     2221 cgccccggta tcgacaagcg ctccgcgccc ggtgttgatt aaaaatgcgc cttgcttcat
     2281 ctctccaatt tgcttctgac cgatcagatg gcgggtatcc gcacaaagcg gcacatggag
     2341 cgtaacaata tcgctgtttt ttagaagctc atcaagctgg acataatctg catccatttt
     2401 tcggctgtta tcataggcca gcacatggca tccaaatccc cgcagccttt tgacgaccgc
     2461 ttgcccgata tggccggttc caataactcc cacagtcata tcccccagtt ctttcccccg
     2521 gatacgatcc aatctgaaat cttgttgcgc cacggcgtgc atggtgggtt ttgcaccccg
     2581 tatggccatc agcatcagca tcaaagcata atccgcaacg ctgtccggcg aatacgcaac
     2641 cgtgccaacc gagattccca ttctctcggc ggcagtcgta tcaatgtgat cgcagccgat
     2701 gctgcgggta gaaatgtatt ttaccccgac ctttctcagc gcaagaattg tcgcctcgga
     2761 aacctcggac ttatggccta cgctaacgca ttgattgcca gcggccaatt ttgcgttgtc
     2821 tgccgatatc gcatcgctaa tcagcgtagg gataatatga aaatccggcg ataaagcgcg
     2881 gaaagcattt gcctcatcct gttcgcatcc aaaaacagta atgcccacac tttttctcat
     2941 tggttctcct cctgaatatc cacactttcc gggaggggat aacagatttt gcatttgttt
     3001 gtataaagca gcttgcaatc tattatttcc ccggtaccta aggcaacttg catccgttta
     3061 acctgggcat attcataaat cccgccactt tcacggacat actggatact gccatttgta
     3121 attttgtata atgcttgggg ctctttgtcg gcggacacct gaccgatgat tttctcatca
     3181 tctaaggtca cccatatttg gtaggtgcag tcggtatcgt ttcgcacttt taaatcaatc
     3241 cagccctctg aaatggttgc atccacccct ttaatctcgt cactgtttgg ctctggaaac
     3301 tcctttactt cgtgacctct gcgctggata attgtcaatg gcgtatgcag gaacatccaa
     3361 aatagtaaat tgctcatctg gcacataccg ccgcccgaca tggtggtgag tttaccattg
     3421 gtcaccgtaa ggccgtcttt atagggggta tctttgtccg catggcgtac cagccaccag
     3481 aaagaaaagg gttcccccgg tcttatcaat aagccgttta aggtctttgc tgccagcttt
     3541 aagttgaaca ccttgttttc ctggtatatc atgtcaaatc cggtattgtg gttgtataat
     3601 gcgcaatccg ttccaaagag caaatgggaa agcgattttt cccctatcgt ctgcgcatag
     3661 cgacagccgt caaatctcat tcccgcataa aagcacattt ttctctgcgc ccgtctcatt
     3721 ggaagcagga acgggaaacg ctgtgtcaat ctttttctgt ccataacata acatcctttt
     3781 ctcatttttc tggttttaaa tattcctcaa ggcaaacccc ttggttgtac atctctgtgg
     3841 cagcttcgat gccaacatat cggtaatgcc acggctcgtt gctcacaccg gttatctctg
     3901 tcttgtctgg tgggtagcga cgaataaaac caaaacgata gctgttttca tccagccatc
     3961 tataaacctc gttgcctgtt gaatgaattc cgtccgcatt gatatccaca gcaagaccaa
     4021 gctgatgctc gctcgttccc ggcacggcca cccaagtttc tgcttccgct ttagcctgtg
     4081 cagaggtgta gcctttcgcc ttatattcgg caattttttc atccataatt tcttgctgtt
     4141 tttctgttgt tcggtatccg gatgcgacaa tcgggtaaac tccatcagtt cttgcggcat
     4201 caaacaaatc ttgaagatag ggagaaatcc gaatatctat ccgctcacca tttgatagtt
     4261 gctcaagctc cacatcgtac tgtgcgggga tggggttctg cctgttcact aaaatcaggc
     4321 tccattcctg ttcatctccc tgttctttgg cggtggcaag agcgttttcc tccaaattct
     4381 ccactacgtc agtgctgctg cctgtatgag ttgcagggat gggcggtatc tgtgttgcct
     4441 ccaatacgga agccagtctc cgccccaaaa acagcgtgca gactgtgaac gaaataatga
     4501 acgcacatag aaaagcccgc ttttccacgg attgtttcat atgccgtttg tgatgatccg
     4561 cattggaatg atagttgctt ttttccattt gatcgaatgc ctcctttgca ataaaactac
     4621 gatttgtggc tggtacgatt gttattatgg caagcgcatc tgtactttcc ctgtagccag
     4681 tctgtagttt tcctgtagca gcaaaaaaag accggcaggg ttatacctgt cggtcaaaat
     4741 catttaagtt ttaaatattt acaatgttga tgtgagcggt aaatccagcc agaacaaaac
     4801 gccatccgag gtgttttcca gcgcatactg gaggctcatg gcatccagcg ttttttgtac
     4861 gatggcaagt cctaaaccgc ttcgcccact ttttctgctt tgcgcctgat caatgcgata
     4921 gaatggggtg aacagccttg gaagcgcagt atcatcaatg tgaacgccca tgttcaaaac
     4981 aaaaaggcgg catttttcag caccaggctc actccatatc cgtacctcgc ctccctgcgg
     5041 cgtgttctga actgcattca atatgacatt ggatagtgcc ttttggagca gcctcggatc
     5101 ggacaggaca atttgcccgg ctggaatatc tgtgacgaac cgctggttgt ttgcctctgc
     5161 caaggtttga aaatcgggca gcaactcggc aaccgtgcgc cctatgtcca acggttcagc
     5221 tatgggtacg attctcccat cattcaggct gacaagctcc agtatttcgg aaatgatttt
     5281 gccctgccta tccatcattt tgatgcattc gcgcagatac ttagaatgat ctttgtagtc
     5341 accgatattt tcaagcattc cctccaacag aacgcttgta gccgcgatgg gcgtttttag
     5401 ctcatgagaa gctgccgcaa agaaatatcg ctgtgtttcc tccaactcat gttcccttgc
     5461 gatttcatcc tccagccttg cgatggtttc tttcagcctg acatacatgg aatgcatatc
     5521 atgagccagt gcgccaagct catcctttcg ctccagcggc ggcggtacat ctttcaggtt
     5581 tgccatttta ttcgcactgt ccgctaaggc tttgatcggc gttgtcattt gccgcgcaaa
     5641 gatatacgcg cataaaaggc tgaatacaac cattatcgca atcataacaa ttccccgaat
     5701 tgtcagccct tgataaagca atcccacacc tgccttgctt tgagcaacaa tcgaaatatt
     5761 atcatctcta tgtaccacat aaagaaagtc gggtctaaaa ctatttgatg tattggcatt
     5821 tggtgtggca tagagtacgc ttccctcttt atcttcaata taaaactcaa aggattggtt
     5881 attgtagtga aacagccctg ccacctcttg aatatcaagc ctatcgctat tctgaatcag
     5941 ttccaccaat ggctgatagg attttactgt ttgctgtaat tccatcactc tgaaataaga
     6001 cacaaattgc tgtgcaaaca gtgttgctgt tacaccgaca agcagtagca gggcaatgat
     6061 cgtataggaa aaaaccttaa tgaaaatccc ttttctttcc attattatga ttcctccaat
     6121 cggtatccta caccgcgaat ggttttgatg atattttccg gcagcttggc ccgcaaattt
     6181 ttgatatgag tgtggactgt gctgccatca ccctcaaagt catatcccca tatacgtgac
     6241 aaaatgattt catgcgttaa ggttctgcct ttgttctgca ccagcagcaa aaggatttca
     6301 aattcttttc gtgtcagggg cagctctgta ccgtcacaaa gtaccgtgaa atcctccggc
     6361 agaagtgtca gcctgccgac acggatttcc tttgccagcg caccgctgcg ccgcagcagg
     6421 gcttccaccc gctttagtaa aatctgcatc ttgaatggct ttgttacata gtcgtctgcc
     6481 tctgcatcaa acgcccggat ttggttttcg tcatccgaca gggctgtcat catcagaatg
     6541 ggagtatcat ttttcgcacg aaattcacgc aaaagttcat gcccgttcat accgggcagc
     6601 ataatatcaa gaataaccag ttgataagtg ttttcgtaaa acttggtgta tgcctcattt
     6661 ccatctgtgc aggcatccac ctgatatcct gcctcagcca gaaacgccct tactgtatta
     6721 cagatatgat catcatcctc gacaagtaga attcgtatcg acataaaaat ccctccattt
     6781 taagaagata acataacagt ctgtagtttc cctgtagttt caaagcgatt tcttttcgga
     6841 ttttaatcat ccgtcattgg gatactcgct tcttttcatg gtgtatgtat ctatattatt
     6901 ggtttaaacg aattaaatca agataataat aattaacacc ttcttaatgt ttgggatgtt
     6961 tcgattacag cggttgtcaa aacatcccga taatactggc aagcaatgga tcagaatatc
     7021 ctttacggat attctgatcc accacttgcc cgcctgcggc gttctcgttt tgcttgttgg
     7081 ggacacccca agcctcagca aaatgcgcgg cctctttttt gcgaatgcac cttgcggtat
     7141 ggcttttcac acaggcaggg cctgtgtgta ctccctccgg tcgctcttgc cgcactccgc
     7201 gcagcagatt cagtcctaat accgttcaag acagtagaaa atcagcaagt ctatattcct
     7261 catatcttca tcatccgtgt ccttgcagtt acaggaaatg taataatcgc caaatgtaaa
     7321 tcccagaata ttttcctcaa aaagccgtac ctccccggta ttaaaatcaa tcaccccata
     7381 ctcaaaggcg cattagacca gcagttatcc aatgtctgga gacttcacca taaagaatcc
     7441 agatgccggc cttttcaatt ctaccatata cggctgatac agcagcagct tcctcgtctt
     7501 tgtttcgggc attttccatt tctgtcatga taaagtttcg cttcttcata catccatgtg
     7561 taaatctttt ctttctgttt cagatgagaa tactttttat cggtctggag cagcctgcca
     7621 tctatcatct tatagttttt ccgtgacaaa ataccatgct ttctctctct tcaaaaccat
     7681 atccaaaatg cgccggtttc attatactat tcagtaaccg cctttccttt ctttgtacgc
     7741 ctgtcaatcg gcttttccgc agttttggga atgagccaga tcgtcgccat ctttacagcg
     7801 ccggggatgc gtcccgcagc acaatagtag tttatccatc gtgggggcac accccatttc
     7861 tcgcctgcct ctttcaacgt catgtagtcc atagtacacc tccacagggt acattataat
     7921 tcttttgctc gaagaataaa agaaacaaaa tgtgaaatat aaactttaag aaaggtgcat
     7981 ataccaacat tccacgggca gagtatgaaa tatacaatgt aagtgggagg tgcgatatgg
     8041 cgaatattga agattgtccc ggctttgaaa ctttcggcgc ggatgtgaaa gcggcccggc
     8101 aagcaaagcg cgtttcgcgc aaagcaatgg cagagaaaat aaatattgac tggcgctatc
     8161 ttgccaacat cgaaaacgac ggggccattc ccagtctgcc ggttgtcata cagcttatta
     8221 aagcctgcgg gcttcctgtg gaacgctact ttaacccgga gatcatgcgg gaggaaagcg
     8281 aactgcggca aagggtcagc cacaagctga aactctgccc cgaagaatac ctgccgatca
     8341 tcgagggcgc gattgacagc gccatactaa ttgaaaagcc gaagaagaat gaaacggagg
     8401 atgtgtaact ggcatcctct gttttacttt tagcgggagc tgcacagcgt agcttctatt
     8461 tttttgcact tttttctttc cagagggcta ttttgcgggt agtttggggc tttggaaacg
     8521 tcttgttagt ggagaaacag taacgtagca agcataggga gtgtagccac tcccggaaaa
     8581 acggcctgcc ccgtccctgc cgggccgcgt ccgttttgct tgttggggaa atccccaaac
     8641 ccctgaatgg aaaggacggt gaagcatggc aaacagaaag cgaaaaatcg tgctgcgcgt
     8701 ccctgtgacg ccggaggaac gggctatgat tgaacagaaa atggcgctgc tgcatacgaa
     8761 gaacttttca gcctacgccc ggaagatgct cattgacggc tatatcgtca acatggacac
     8821 cagcgacatc cgggcgcaga ccgccgagat acagaagatc ggcgtcaacg tcaatcagat
     8881 tgcaaagagg ctgaacggta tggggccggt gtacgcacag gacatcgagg atataaaggg
     8941 ggcgctggcg cagatatggc agttacaaag atacatccta tcaagtcaac gctgaaaaaa
     9001 gcccttgact acatcgagaa cccggacaaa actgacggca agctgctggt ggcctccttt
     9061 gcctgctctt atgaaacagc ggacattgaa tttgaaatgc tgctggcgca ggcataccaa
     9121 aagggcaata atctgggcca ccacctgata caatcctttg cccccggcga agccacgccg
     9181 gaacaggccc atgagatcgg gaagcagctt gccgaggaag tgctgcaagg gaaatatccc
     9241 tatgtgctga ctacccatat tgataagggc cacgtccaca atcacattat tttctgtgcc
     9301 gtggatatgg taaatcagcg caagtacatt tccaacaaac agagctatgc ctatatccgg
     9361 cgcaccagcg acaggctgtg caaggaaaac ggattgtccg tggtgaagcc cggccagagt
     9421 aagggcaaga gctatgccga gtgggacgcc cagcggaaag ggacgagctg gaaagcgaag
     9481 ctcaaagcag ccattgacgc cgctatcccg caggcaaaag attttgatga tttcctgcgg
     9541 ctcatgcagg cgcaaggcta tgagatcaag ccgggaaagt ttatttcttt ccgcgctccc
     9601 ggacaggaac gctttacccg ctgcaagaca ttgggggaag cctacaccga ggaagccatc
     9661 aaagagcgta tcaaaggccg cgttatcacc agagcgccca aagagcgcaa ggggatttct
     9721 ttgcggattg atctggaaaa caacattaag gcccagcagt cggcaggcta tgagaggtgg
     9781 gcgaagctcc acaatttgaa gcaggccgca aagactttga atttcctgac tgaacatggg
     9841 atagacacct acccggattt ggaaagcaaa gtggccgaga tcaccgccgc cagcgacgag
     9901 gccgccgcta ccctcaaagc tatggaacac cggcttgcgg atatggcggt gctgataaag
     9961 aatatttcta cctacaagca gctctggccc gtggctatgg aataccgcaa tgcagcggat
    10021 aaggcaaaat tccggcgtga gcatgaaagc acgttgattt tgtatgaagc tgcggcaaag
    10081 gccctaaagg gacagggtgt gaaaaagctc cccgatctgt acgccctgaa agccgagtac
    10141 aagcgattgg ccgaggaaaa ggaacggctg tatgagaagt acggcgaagc gaaaaagcag
    10201 atgcaggaat acggcatcat caagcagaat gtggacggca ttttgcgtat gacgccggga
    10261 aaggaatgga cgcaagagct atgagtattc cacgaatgaa ttaccctcaa tatcgcaagg
    10321 cccggaagct gacgcatgag tgctgcaact attgtgacgg caactgcctg ttgctggacg
    10381 acggcgaaga atgtatttgc gtacagagca tttcctattc gcttctgtgc cgctggttta
    10441 aggccgctgt cctcccgctg gatgcggccc tgtatgccga gatcaccaaa gaccgggacg
    10501 aagtgaaacg ctgctgtgag tgcggggcca cgttcacgcc gaagtctaac cgggccaaat
    10561 actgcgcggc ctgctccaaa cggatgcgcc gccgcaaaga ggccgagcgc cagcggaaaa
    10621 gatactggaa acctacgcac ttagagctgt aaaagccctt atatttcaag gcttttccgg
    10681 catggtcagc ggggtatgtg ggtgttttat cctatccccg ccgaagtgct gttctaaatg
    10741 cgtagaattt accagcagaa aagcgccgtt ttctctggtt ccatgcttcc gaatatggta
    10801 tcatagaaaa aatcagtctg acgggggtat caggatggac ggacaagaaa agctgctgga
    10861 ctatgaaacg atcaaagccg ccgttgccgg tgagaggtgg gccacggaaa aagtgttggc
    10921 ccattatgct gactacatca acgagctttc cacggtggag atcagacagg caaacggcaa
    10981 aaagaagaaa gtcattgacg aagatatacg gcagcgcatt tccctgaaac tgctggaagc
    11041 attgccgagc ttcccgctgg aacagagata gacggcaaga gggctgcacc ctcttacgcc
    11101 gctttgcggc tatcacctgc acacccctga aacagataca cccgctgcgc ggctatatct
    11161 gtttcagggg tctaattata gctgcaacct acgcatttaa gggattgaaa accgcccgct
    11221 gcaaggcttc tcccgcatgt ttgcaggggc aagcgaatac ttcatcctat ccccgacaat
    11281 accgggttct aaatgcgtag gaataggcaa aaagaaaaga cgccggacgc aagcgaccac
    11341 atggccgccg cgcccggcgt ctgctgttcg ttattcgtct gtgaagtgga acttctggcc
    11401 gtcatagtcg gcatccgtca tgcgttccag cttggaaagg gtctgctggg cctgcgcccg
    11461 catatcccca tccataacca gcatgacggc ggtgagcctg tttatcgttc tgcgccggtc
    11521 tgcattgtgg tacatacaga tcaggttttc ttcctccacg gtaaagtgca tcattcctta
    11581 tacctccaat tcattctttt tggttctggc cgccgccttt tttgggctgg cggcctgttt
    11641 gctgtcggat agctgcttgc ggatagaggg ccggggcctg cgaatactca catcccggcg
    11701 ctcgtcccgg ttaatcacgg catacacgcc atgcctgcct ttcatcccgg taaaacacag
    11761 ggatttatag ggcagcatgg caaagagccg gtcattatcc ttggtggaag caatcgccgc
    11821 aaagtgcggg gagatctcca ccatgaaatg cgttttattg gggctgttag gcgcggacag
    11881 atctttcagg ctgtccacaa tacgccgggc ctcggtttca atccggctgt catgcaaagc
    11941 ggtgacgtgg actttgaaat cgccggggac ggcccgcccc cggctgtgct gctccgtccg
    12001 tagcgcctcc tgcagcacag ccgggtcatg gggctgggcc tcatagtaca aaaagtttcc
    12061 caatacccgg tcagggcttc cgtcaaaacg ctggctctcc gggatgtcac gggtgccatg
    12121 ctcataaaag agccggttca cactcaccgg ctgggcggtt tcaatgacgt gccggaaatg
    12181 ctgcggatag tccagctcat agagattgcc ccggatagtg ccgccctgtg tgccggtcag
    12241 ctccaccgcg taggccagta tcttgtcacg ggtctgctcc ccgtaaaatt tccatgtgtt
    12301 gtactgtcgg gtgtctttca ggaaaacatc acgttcccgg aaacagtacg tcccggaggg
    12361 ccgggacatc cagagcaggg tcttgtctgc ggcctgatcg ctggcggcat cccgcctgat
    12421 aatgtctttg tcaatgccaa agtcactttg ataaaacgcg gtgttctgcc gcatgatctg
    12481 ttccagcgcg gccagaacgt ccacattttc aaacttctgc atttactcta cctccaatcc
    12541 ctttgatttt tcccgcgccg gtggctgctt ggcctgttcg gtcttggccg cgttgagctg
    12601 cgcccggata gagggctttt ctttctccct gcgggccgca agctgtaagg cgctgggctt
    12661 ttcgccggaa aggggctgga tgcacttgat acggtcagcg acgcggattg tggtgtcgtt
    12721 taactgccgc tgccatgccc cggccttggg cgaccagcgg aaaccgttgc ttttcagctc
    12781 gtcgcgtaca gcagcatccg gcttcccctc aaagaagatt tgcaggcggt tggccacccg
    12841 gttgatctcc acagtgccgc cgtcaaactc ccatcccaca taggccgcct ccttttggcg
    12901 ggtcaattcc tcaatgcggc cctttaaccg gcgtatctcg gcgttattgt tggagagctg
    12961 ccaggactgg aacggttttt tctcatagtg ccagcccgac gccatatccg ctttcaggtt
    13021 ttcaatgcct ctttcggtca gatgggggca tccgtccagc gtcccatgct tgcggtagta
    13081 ggcattgacg gccttcatca actcctgcga cgcttccagc ttttccagtt tcttttccag
    13141 cttggaaacg gcctgcggat cgtcggcgct gataccaccc atgccggtgc tgcggatttt
    13201 gtcaagcaag ccctgaatgt actgaaattc ctccatgttg cggtcactgg cggcgttctg
    13261 cttttctttc gcccgtaccg ggaaattgga cggcccggca atcaggacag aggggacgcg
    13321 ggcggtaatc tcatagccct tattcatgtt ctcggccagc ttgcgggcgt aggtgtcaag
    13381 gaggctgtct atcttttcgt gatactctgg ctccacacgc tttttctggc gctccgcaat
    13441 ttccacggcc tgatcgacat agtgccggta ttccgccgtg gcgctgccgg gccggtagtc
    13501 agaaaagctc atggcttcct tggcgcggcg ggcggctccc tcattgatgg gataatagcg
    13561 gacagccgtt tgcgggggcg cagcctccgg gggttcctcc tgtgtctggc cggaagcaac
    13621 tgccacatcc tcaaaagata gctgttcggg ttcctccggc tgcggttcgg gcggcgtatt
    13681 gttgataatg ccgtcaatca tgttttcgtt ctgctccgta gacagttcgg cggtacgcag
    13741 gggattttcc cgcaaaaact ccggcacttc catatagccg aagctgtcta catagtgggc
    13801 ggtgtcctgt ccattctggt gcagcaccac cacgtcggaa acggaaaggc tgtgtcccct
    13861 aaaatctgcc gggtggtcga tgttgaatgt gcggtaaata tcctccaaag acgtgctttt
    13921 atccagcggt gccgtataaa caaggtcata gttggcactg tccacggcca gccctgcggc
    13981 ctgtaaacgc tcgtatggct caaaccgata gtcgcgggtt tcctccccgc ctcgaagctg
    14041 gtagatggaa aaggtgtcct gttggggtgc ttcatagcca gccgccgcgt attcctccac
    14101 ggtcataccg gctgcggcgg cctcctgcgc gatctcggcc tgtacctgtg ctttgtagtc
    14161 ctgcaagctc tggtcgaagt cagccagttt cggcggctcc ggcaactgat actggccctg
    14221 attgagcatg gggcggcact cctgcacata agagagaagc gcccccaaat aggccgtttc
    14281 ctccggcgtc agggtttccc cggactgtaa gcggccctgc gccgtctgtt ccatttgggc
    14341 aaggctgcaa tgcagcttca tataggggac aaactcgcct aacagcatat cccgatgggc
    14401 cttgtcctgc tcccagccct ctatgccggt tgtagcgatt acatgctttt tccagttttc
    14461 gtcctgcgcg taaaattggt ggtagctctg gatatgttcg atcagggaac cgtccctatc
    14521 tccaaaatcc tgccgcccct cgtagctgtc cggctcgccc tgaaagatac agtcaatgcg
    14581 gaacgcggtt ttgtcatagc ctgcctctgc cggatatgct gcatccagac gggcaaacag
    14641 cgcgtcggcc tcatgcaacg gcatttgctg gccgtccttt agctgggggc tttcgctcca
    14701 tagcacagtc acaagcggct cggctgccgg atcaagggcc ggggcctgtt cttcctcggc
    14761 ctgcgccggg gccgctgcct ccggggtaac gtcaaccgtg acggcctcct gttcctgccg
    14821 ggcctcgcgc tcctgctgaa gctgggcaaa atggccgtcg atctcggtta tcaattcgct
    14881 ggccgtggcc cggatggttt caagggaggc tttcagctcg gataactccc tgccgctgct
    14941 ccaatcggcc acatagccaa aagaatagtc cgacgtgtcc agcccgtaat actggcagac
    15001 ggtataggcc acgctttcgg cctgtacctc gcgggtgctg cggtctggcc gggccgcctg
    15061 ttcctcggcg ggggcctccc ggtcaatggc gtggagcttg gcatgggcaa tctcatggat
    15121 ggcggtcttt agggtctgca attcgctcat gccctcgttg atggcaatgc gctgttttgc
    15181 gtagaagcag cggcccttgg ccccgtctga aatcggctca atgcttatgg gatagggtga
    15241 tgtccgttcc agcgcggcaa agaaatcctg atactgctgc acgtcgccgg taagttcact
    15301 gatatagggc atgggaaaat ccttgccctc ggtctggctg atgtcaaaga ctgtcaccac
    15361 cttaaaggcc ggaaccgtca cttcctgttc ttcggtcacg ggcttgccgt ccgcgccgat
    15421 cacgggctgc tgggtggccg ggtcgatttt ctccatctgc tgcttaacct tgaacggggc
    15481 cggggccagt atcttaatgc ctttctcgcc ccgcttcaca tggcggtcaa attcatcctg
    15541 ccacctgtta tagcccgcca caatcgaacc gccctgcatg gcgatcagca gcgtattgtt
    15601 cagactgtaa tcgtgcatct tggacatagc ggataagtaa gccatatagc ggctactttc
    15661 aaaaagctcc tgtacgccgg tttccagacg gtcagtaatc actttcatgc gttctttgct
    15721 gttcatgccg tccagcatga tcgggataac cgggggcggc gctttgtcgg ggctttcgga
    15781 tgcctccggc tgctgggcct gcggttcctc ccggtcagct tccggctggg ggtagtgcat
    15841 cacccggtat tcctccggca ccggctggcc ctcatggacg cgctgccatt cgtcgccgct
    15901 tctcaccaga tagccgtact gggtaaaaac gccttgttcc tgctgggcaa tgtggcgtcc
    15961 aaactgcgcc gtatcaatcc cggctttcca ttcctccggc atatccacca tgccggaacg
    16021 gttcaggtag tagtcgccca actgtccatc ccccctaacg tcgggaagat gcacatagta
    16081 atccacattt tcggtgtagt cgatgatctg gccgatattc tcaaagccgc cacggggatt
    16141 ttgcagggcg gcgttcagct ctgcgatctc ggcggcgtca agctggccca gccgggcggc
    16201 aaggaaattc agctcgtcca cgtcggcggc cagcaccatg tcatagggaa tggcaaggtg
    16261 tctgtcctcc gggcaggaat acccgttgat aaaaaaatcc tgcggattgt cggcagtaat
    16321 gccaacatcc cgcagggcgg cttgcagcgt ttcggttgtg gtgggcaaat ccagccagac
    16381 gccttgtttg ccctgttcca aaaactgccg gttatggact tggatagaaa atacttcgtt
    16441 cacgcggtca ctcctttctc tgttctgttt caaaaaatag ggggttgggg acttccccaa
    16501 caagcaaaaa tccccggccc ggcagggacg cggccggggg attttaccgg gagtgtagcc
    16561 acactcccta tgcttgctgt gttactgtcc tttagctcct gtgttcaatg cggtagttga
    16621 cgtacttgcc cccggtatca gccaaaagca ccgtcccgtc gtaggttttg ccggtcttta
    16681 cggaatagag gccctttacc ttgactttgc cggttttgag cagcgcagcg gcgatcttcg
    16741 gggtgaacgc tttcttgcgt tcctcaaaaa atcggtcatt cttccacatg acgaactggc
    16801 aggcccggtt tccgcagtag tagtttttct tgccctcata gacagcctca ccacagcggg
    16861 gacacgtccc gataggaaca cgctcggcct gaaacaattt ctgcttttcc tcactgatac
    16921 aggaataggt ctgcaccagc tcccgcgcct gcgtctcgat gccgtccata aattcgtcag
    16981 gatcggcctg ccccttggcg atctcggtca gccggttttc ccattcggca gtaagctggg
    17041 gagaagtaag cgcctccggc agcaccacag ccagattgat gccgtccttg gtgggaatga
    17101 gctgcttgcc ttttctctgg acaaagccca tctgcaccag cttttccaga atggcggcgc
    17161 gggtggccgg tgtgcctaat cccttgcgct cggcatcctc cggcatatcc tcggccccgg
    17221 cccgttccat agcggacaaa agcgtatctt cggtgtaggg cttgggcggc gtggtgaaat
    17281 gctcggtgac agaggcggcc acgtctgcaa aggtctggcc ctcgcaaagc tccggcagct
    17341 cccgcaccgc ctcggcgtcc tcgtcggcgt cggtctttaa ggaagaacgg aaccggcgtt
    17401 caatctcttt ccagccgggg gaaaggacgg tcttgccctt ggcggtaaac tcctgcccgg
    17461 cacaggtaaa tgtggcggtg acggcctcat agatatgcgg cccggccacg gcacataaga
    17521 gcttgcagca gatcagggac agcagctttt tttcactctc ggccagagtg ccaaagccct
    17581 gcttcacaaa ttcagcggtg gggataatgg cgtggtggtc ggacaccttt ttgctgttca
    17641 ggatgcggtc aaactgcggg gaaaggttca ggcccttggc aaacggcacc agctcccaca
    17701 ggccggacac aaggctttcc acggcgggca gcatatcgtc ggtaagatat tgactatccg
    17761 ttctcggata ggtcagcagc ttctttagat acagtgcctg ggcatagtca agggtctgct
    17821 tggcggtata gccgaaaagc cggttagcct cccgctgcaa ggtggtaagg tcatagagct
    17881 tgggcggctg ttccgtcttt ttctcccgcg tcacggaaac gcaaacggcc tgcgcttttg
    17941 cacaagccgc cttgacggtg tttgcctcgg cagcgtcgga aaagcggctg cttaccgcgt
    18001 ccgcgccgcc cgccgtgata tggacgatgt gatatttctc tttcttgaaa tcggtaatct
    18061 tggcgtccct gtccaccagc atttttaagg tcggcgtctg gacgcggccc acagtcaggt
    18121 tcttgtggta caggacggaa aaaagccggg tggcgttaat gccgatcagc cagtcggcct
    18181 tggcccggca cagggccgat tgatacagcg gatcatagtc gccgcccggt ttcaggtggg
    18241 caaagccgtc cttaatagca ctgtcctcca tagaggaaat ccaaaggcgc tcaaagggct
    18301 tttggcatcc cgccatctgg tagacgaaac ggaaaatcaa ttccccctcg cgcccggcgt
    18361 cggtggcgca cacaaggccg gtcacgtcgg ggcgctgcat cagcgaacag agaacgtcaa
    18421 actgctgttt cttgtcgtcg gggacgatgt actgccatgt ttccgggaga atgggcaaat
    18481 cctcatagcg ccattttttg aaacgctcgt catagcgtcc ggcgtcggca aaggacacca
    18541 gatgtccgat gcaccagctc accagatagc cgttcccctc caaatagccg tcgtgtttct
    18601 ccgtggcccc gatcacgccc gcaatgctgc gggccacgct gggtttttcc gcgattacaa
    18661 gtttatatgt cattcctcgt cctcctgttc actcacaaaa tcagcttccg ggtcaaactc
    18721 ggtttcgggt tcctcgtcct ccaaatcctc gtcctcccca aaatcatact cgcccaaatc
    18781 gtcgctgccg gtggtttcag ccttgggctt tttgaacttg aaatagtaaa gggccgcacc
    18841 gccgcccgcc agcgccacca gcaggaacac gatcaggccg cccgcgccgc ccttgggttc
    18901 ttcctccggg gcttcgggtt cctccggctg gggttcctgc ggctccgggc cgctgcactc
    18961 ggtcaggttg gttttacata ccgggcaatt cgtgttcaca gccccggcct tgcatttcgt
    19021 ggtacaggag cagacagcag cgggggtgac ggtttccccg tcctccatca gcgtcagcag
    19081 atcggcttca tccacctgat ttaagaaatg gacagtttct tttccctcgt cgtcccggtc
    19141 aataatcaga taaaacacat tgccgttttt ggtttccacg gtgataaact gcttgccatt
    19201 tttcgcggcg ctgccgatgt cgtcaatcag tgacaggttc ccgtccgggg acagggcggc
    19261 gctggccccg ccgccattga acagttccag cagagaagca atttcctccg ggtcaatgga
    19321 gagggaaaaa ccgccctcgc cggtagccgc tacttccttt tcgctgacgg tcatatagtc
    19381 ggaatggaca tagccgatgc gctccggcaa gagaatttta atccagtcgc cgtctgtgcc
    19441 gactacctca accgtcgtcc cattgggaag ctgggtgaac gcggtcttgt caagccccgc
    19501 gccggtgcgg acattcagcc tgccgccgtt ggtggtgacg gtgccggtct gttcaggttc
    19561 ttttttgtcg gtgccggtgg tgatctggat gccgtcgtcc gtggcggcaa tgctcacctt
    19621 ggaaccgaac agcgcggaaa acaggtcggt gatgtccttg ccggtcagag ggccggtatc
    19681 tttttcggcc tcggtttctg tcccgtccgt ggcggtgccg gtttcgtctg tttcaggctc
    19741 caaaccgccc gtagcgggtt cggttgtagc ctccggcagc ggggccggtg ccgggtcatt
    19801 gccgcctgca agggcggtaa cgggaaaggc cgtgacgcag agcatcacgg ccagaagtgc
    19861 ggggagaata cgggaaatcc gtctggtctt attcttcatg ggcattgtcc tcctgttcat
    19921 ggtaaggggt tgcggcaggg gcggcctgca ttgcgccgct gcgtaagaac gcggcaaatt
    19981 cctccggctt catgttcatg ccgcgcacaa gctggatgat ctgcaaattc tcctgttcgg
    20041 ttttctgcgt ttccagctcc cgaagctgtt tctgaaactc cgcgatctta gacttggttt
    20101 tctggatgtc cttttcgatg cggtcaagtt tggttgccat aattgaaagc tcctttctgg
    20161 cgttaaggta aacgcccgta actgtaaaaa tgggattgcc agtagtttga agtaaggttc
    20221 gtgtaggaaa tcgggttccc gcaatgcagc atcacattgt tgcccacata caggccgacg
    20281 tgggacacgc cgggggtgtc gtaggttccc ttgaaaaata caaggtcgcc gggcttggcc
    20341 tgcgaactgg aaacaggggt gcagatgtta tataggccct gcgcccccag ccgtcccacg
    20401 ttccatccac tatgattgat tacccacgaa atgaagccgc tgcaatcaaa gctggtgctg
    20461 ggagagctgc cgccccacac atagggataa ccgatatatt tttccgcctc tttgagcatg
    20521 gcggcaaagg tggcatcctc caaagcctcc gggggtacgt cgtagtaggt cggcggcgtc
    20581 agggtggagg cgttggggta ctggctgccg gaaaacagat caggccggtt tcctagcgtg
    20641 gacatataca gggaatagaa acttagcgtt tcctcgtcca tgatatagac tggcacatgg
    20701 gacaggtcga agttttccag cgtgacgtag cagatgtaat agtcatacgg cacttggtag
    20761 gtgtctgtat gggtgttgcc ctccgcgtcc gtccatgtat cggtttcggt gcggtaacgg
    20821 gtttctacca cgacgttttc tgtcaggata tattgcttgt caaagagcat ggacaaatca
    20881 ccctgtacct ccccgatggt aaatatcccg tcatggagcg ccgacagaat ggaaatcagc
    20941 acataggggt catggctgat ctcgtcaagg tcaaaatggt attcgtcata gtcatgggtg
    21001 gcctcgtagt tgtccagata gtcttgcagc tcggcttcca gctccgcgta ggcgtcctcg
    21061 gcggccaaca tatcgctatc ctcggaaaag taagaggacg ccgtgatgcc gcttccggcg
    21121 ctgccgaaca tggcggtgca ggactgtaag ccgccgatga taagcagcat caaaagcccc
    21181 aacacaagga cggccagcgc ccctttccag tggcgggcca caaaggaggc cgcccgttca
    21241 ctttctttgg ctgcggcctt ggccgctttc gtggaggccc cggctgtctt ggccgcgccc
    21301 tgtgccgccg cgcctgcggt ctggcccgct gtccgggcgg ccttggcgta ctggcgcttg
    21361 atctgctgtt tctgccagaa ccgggaaatg gggttgctgg ccgcctgtgc gatctccggg
    21421 ttatcccgca aagatttctg gtagaaatac tccgcgttgg cagacaatag cctgcgctcg
    21481 gccttttccg cgtcccagta gggtttcagc ctgtggtgcc ggatggtgct gcggatggct
    21541 tttccggcct gccgctcggc aagttcctcc cccttatggc cgccctccac acccacgttt
    21601 tcatgctcga cttcatggat tttcccgtgg ccgaatagaa aaacctcgga aacaggacgg
    21661 cctgccggat tgggtttcag ggagggaggc cccttgtcct gtttgtcaaa gtgcaggacg
    21721 gtctttgctt tgccgcttgc ctcgtcaaag acggtttcct tgaccgggac gcgcttcttg
    21781 gggatggcgg cgcgggcctt atccagctta tcggcccgtt tgtccgattt tcggatatag
    21841 ggctgtaaat ccgggtcggc ccgttcttcc tccgacagtt ggagccgggc ggatggccgg
    21901 tgcagggcag cgtccccctc ggcggccaca gccccggcct gcttcgcggc tttcttggcc
    21961 ttatggccgt catggatggc gtcggcccgt tccagcagct tatcggcggt gccgcccggc
    22021 cccggcgcgg acaaatcctg ttcggcctcc cggctggaaa tgttctccac ctcgccggtg
    22081 gcctgatttt ccagcgtcag cccgtcgcgg gtcatggcct gcgtcacttt cgccttggct
    22141 ttcagctctt tcatggtggt tcacctcctt tgtggcgtcg ccgggtcata ctcccgccac
    22201 ctcgtttaag cgggtcgtca taatcttgta aagctgggtg ttgtgcggga tggggttggt
    22261 aaagggcagc accacgtttt caaagatcaa aaggccctcg ccgggttcgg aattgtcgat
    22321 gtacttctgc tgttcggtgg acaaattcaa gcgttccgca aggatttttc tatccccgga
    22381 cgcctgattg agcagcgtaa tgaagtcgct gttttcaagg atattttcaa tttccgggga
    22441 ggaaagcagg tctttgacgt tctgggtggc acctgttgga atgccgcccc atttcctaaa
    22501 ccgcttccaa atctccgcag aataggccgc cgtctgttcc tctttcaaaa gcaaatggaa
    22561 ctcgtcggca aaataccatg tggcctttcc ttggcttcgg ttggccgtga cgcgccccca
    22621 tatctggtct tggacaatga gcatccccag ctttttgagc tgcttgccca gctccttaat
    22681 gtcaaaggac acaagccggt tgccgatgtc cacgttggta cggtggttaa atacgttcag
    22741 ggagccggac acatacaggt caagggcctg cgccacccgg tcagcctccg ggacatgctg
    22801 atctaacaac gctttgtgga ggtcggacag gatcggcata ttctccgggc gggggtctgc
    22861 cagatagggc cggtagatca cgcgcacagc ccggtctatc acggtctttt caatggcttc
    22921 cagcccgttc ttgccgccca tgacaagctc acagaacgac agcacaaagt cggatttcag
    22981 ggccagcggg ttatcgtcct ccgaataatt caggttgatg tccagcgggt tcacaaagtc
    23041 cttgctggta ggcgacagcc ggattacctg tccgtggagc cgtttgacaa gggggtaata
    23101 ttcggcctcc gggtctgaaa taaaaatatc gtcaggcgtc gttaagaaca cactgacgat
    23161 ctcggattta cagctcatgg atttgccgct gccgggggtg ccgagcttca aggcattggg
    23221 gcaacgggcc tgcttcctgt ccagcataat catgttgcgg gacttggcgt tgatgccgta
    23281 atacatggcc gcgccgccct gaaaaagctc ctgcgtcaca aaggggacga acacggccac
    23341 gctggaagtc gtaaggctgc gctgtatctt gatctgattg acgcccagcg gcaggctgct
    23401 tgcaaggccc tgttcctgct ggtagtccag ccggacaagg ggacagttat gcttttgggc
    23461 cacgcctgcg gcttggaaca cgtcgttatt cagcttttgc ttggtgtcgg ccacgttgag
    23521 gacaaggaac gtcaactgga acagccgttc attgcgggtc tgcaactttg tcagcagctt
    23581 cttggcgtcc tcgccgtagg tggcaaggtc gctgggaaga atgtccatat cgtaaccgct
    23641 gcggacggcc tttttctgct ccgcaatctt catagcgtca aggtcggtaa ttttgcgctt
    23701 gatggtcttg atcgccttgg tctgttcaat ggcctgcaca tggagattga ctaaaacacc
    23761 gctttccgta ttgaggaagt cggccagcat atcgtccgac agctccgggg acaggatttg
    23821 caggaaactt accgcgccgt atttgccgcc catgccgaat gtctttgcat tgccgaagca
    23881 cagggaggac ggggcaataa aatccttggt ggagaggccg gacggggcca gccacttcca
    23941 gtcaaaattg aatatctcgc cgtccgggtg gtacacgcca tgcagcaggt tcagccgttc
    24001 ccagccgtcc atcacatggg ctacggcccc catgctcttg aaatagccta acaggtcaaa
    24061 gccgatgcgc ttcaatctgg ctcgcgccgc tttcacgctt tcggcctcga tggtgaatgt
    24121 caggtatttg gtctttacca tgccgttgtt gccgtttgca agctgctttt gcagaatgcc
    24181 ggtgtactcg gcccggatgt cgtcgaagtc gtccccctgc ggcgcgatct caaagctctt
    24241 ggcgtactgc accgggtcaa ctttccgatt gaggaaagaa aactggatat gaatggaggc
    24301 gtccacatag ttatacaggt cgcacagttt ttcaaagata gccgtctgcg tgtccggctg
    24361 ggcaagctga tagctgatgt ccgcaaactc aatgcacttg gaaaagacgc cgggcgatac
    24421 ccggcacacg ccgtccgggt acatgacctc gtaggggatg ctctgctgcg ccgtgtgggg
    24481 cctgccgtcg cccttatatt tacggattac cgcgtcaatt tcttttcgct gggcgcgggt
    24541 cagctttgtt ttttcggttt ctcttgggat ttcctttttg gacaatggcc tttacctcct
    24601 gttccatctg gtattgccgc acaagggcgg tgtaggtgtt gtcggtctga tagggacgct
    24661 cctttggctg gatgaagcag cactggatga tctgcataat caccacttca aggggctggc
    24721 cgtgacgctc gtacatggca agcaaaaagc ccggcagcat ggcaaatacc atgagcaggg
    24781 ccgccgcgct gttggtaaga tagtcacggg tgagaaaata taaggggacg ccgatcacca
    24841 gcgccccgga aaaacagatc agttgccgtt tggtaagccc aaacacaacc ttgcttttca
    24901 catgggttaa gtccttgggg acggttacat aagccatttg gcacctcctt tcacgtcagg
    24961 ataaggtcac attccacctc gttcccccac acgtcccagc cgggggtttt ctgtctggca
    25021 aacagctcca cgcggggcaa atcgcccgcc agctctacga ttttttcacg ggtcacatcc
    25081 ggctttttgc tgtgttgttc aatcgggctg ataataaact gatggacaga ggcagaacga
    25141 cgcttcggct tgccgtgtgt tgccagcagg cagatttccg cgtttcccct tgtccagcgg
    25201 cccagcccgt aaaaccatgt aaggctttta cggttctgtt tcagccatac aaaagccacg
    25261 gtcttgaatg aaaagcccca cgctttgatt aaccgcagag cttccggcag catggggaat
    25321 gtggcccaca aaaacagcag gcagtctttg gacgccagcc gttccaccgg cagggcgcac
    25381 agctcgtcaa tgctcatggt ggaatagtga ttttccgctg cgccctgtac gtttttgcag
    25441 tcataccgcc acggcgggtc agcgtaaatg atgttgtacg ttgaaataag caagttcctc
    25501 ctgtgtatgc ttggattttg ggatcaatgc gccccgaata cggcctttga tatactgccg
    25561 gtcttgaaca ggcagaagca cagcagcacc gtatagccca tacagcccca gattgcgccg
    25621 gatatgtcgg aagcggaacc gatattctgt atcagcaccg cgtagatacc caccacaacc
    25681 atgattaaaa aggcttggaa gcccagcgcc agcagggatt tcagataccc ttggcccatg
    25741 ccgcgccatt ccgcgttgcc cattgtggca agggggatag gccccagcga cgtgacaaca
    25801 tagatttcca ccatgcggcc ataggtcaca aggaaaatgc agatggaaag gatattcatg
    25861 gtgaggccca caacaagggt ttggaaccac agccccaaaa gagcgcccac gctcatagcc
    25921 tccaattggc tttcaaggtc tggtatcagg gtgttaaaat caatggatgt ctgcccgata
    25981 atcacaccgg cgctttgatt gacgacgctt tgggtggcgt cgaatacgcc catgacgatg
    26041 ttccatgtgt tcgttacgat caggattgca aacgcggatt tgaatatcca gcggaagaac
    26101 atggagctgt caaagtcgtg catattgttc ttttccgtta tcatctggat aagctcatag
    26161 cacatgacaa gggcgagtat ggccccggct atggggacga tcacggtttc cgacaggctg
    26221 tgcagcatat tgaaaatacc gctgttccat gcctgcgggg ttcgtcccac gtcggcggcg
    26281 atttctccga ctttggcatt taccgtatcg aacagcccgg acaggttgcc cgtgataccg
    26341 tccatgagga tgccttttat ccattcttca atgatttcac taatcacggg tagcacctcc
    26401 tttcccgcgc cctcccggtc tggccgggag gacgcggagg cgattagtta agggtttgga
    26461 cagggacaaa ggccaggatc agcccagcag cccggacagc aggggaacca gagtgacacc
    26521 gatcagggcc acgccgccgc cagcgacaag ctgcttgaca ccctgcgatt ttgcccccgg
    26581 attgtcctgc ccgtatccct ccaacagatt gatgccgccc catacgcaca gagcgccgcc
    26641 cagtccgatc acgatagtct gcaaagtagt aactgcggaa ttaaaaaatg ccataaattt
    26701 acctctttct gccggtttcc ggctcataat gattttttga ttttgttgtt ggaatgaaaa
    26761 aagccgctgt ttattcagcg gcggcgtcct cgtccgacag gtcgatttca tacaggtcga
    26821 atacttcatc ctgcgttacc acttgctgcc gtttccggcg cgtgatagtc aaaaatctgt
    26881 ccacgtcaaa ggtattcttc ttgtcggcgt cggcaaggta tttgtaccgg ggatgcttgg
    26941 taatatcgaa tttctccgat aaaaacgggc gcacaccccg tagctgcaag atacacttgc
    27001 cgccgtccat cacggcaatt tcgtcttggg acatcaactc ctttcctaat ttttgatagt
    27061 tcaatccgtg ggacgtttgg cttccccggt tctcgctggt gttgaaactg tcaatcgttt
    27121 ctttccccag cgcatcggaa atctccttgg catttttgcc gcgcccacta aggaaaagca
    27181 cactatcgca gttatccgaa atgatctcgg cggcgtcctt gtaaatcgct tttagctgcg
    27241 actggctctg caaaataata gaggccgata tttcacggct tcgaatggtg gcaatgagtt
    27301 tatcgaaatt gggtatttgg ccaatgttgg caaattcatc caaaatcagg cgtacatgga
    27361 caggcagccg cccgccgtac acatcatcag cccggtcaca aagcaggttg attagctggc
    27421 tttggagcat agccagaatg aaattaaagg tcgtgtccgt atcgctcata atgagataca
    27481 gcaccgtctt ttcgtccccg atggtgtcca gctccatttc gtcgtcctcc atcagctcgc
    27541 gtacctcccg gatgtcaaag ggcgcaagtc tggcaccgca tgaaatcagg atggaagaac
    27601 gggtcttgcc tgcggacagc aggaatttct tgtactggcg caccgcaaaa tggtcggggt
    27661 ctttctgctc caaacgctca aacatgagat cgacggggct ttgaaactcc gggtcgtcct
    27721 cgcgggcttc actggcgttt atcatttcca gcagcgttga gaagttcatt tcctccgctg
    27781 gggcctcgta ccagatatag ccgatcaggg ccgaataaaa caaccgttcc gacttcaccc
    27841 aaaaatcctc cgctgacttt tccccctcgc ccttggtgtt ggaaatcagg gtatttacca
    27901 gctttagcac gtccttttcc gaatggatgt agacaaaggg gttgtacttc atggacttgg
    27961 aaaagttaat ggtattcagg attttaatgc ggtagcccgc ctgctggaac atcctgccgg
    28021 tttccagaac caaagtgcct ttcggatctg tcaccacaaa cgaagtcgga tagtccgtgg
    28081 tcggaaagca ttgcatcagg ttgggtttca cgaaaaatct cgttttccct gaaccgctgc
    28141 caccgatcac cagcacgttt ttattccgcg ctgtcttggg gtctttgggc ctgccggtca
    28201 tggtaagccg ttccgtttgg gtgagcagga tattattttc aaacttgggg tcgatgtaag
    28261 gcgctatgtc tttggccgtc ccccagcggg cgctgccata ctccatgcct ttgcggtatt
    28321 tcttcacgtt cttgctcttg cagtacacca ccagccgcac gatcacggca ccggcgatac
    28381 cgatacacag gtcaaacagg tgcaggcttg gcagcgggtt ggcaaaagcc gccgcaaaac
    28441 catccgcaaa atgcaggagc ttccccgaca ggtcagcacc agcggccagc cggtacgcct
    28501 ccccgcattt cgcaaacagg tacacaaaaa tcagatacgg gaggttcagg atcagcaatt
    28561 tctttacgtc aggcttcaca gttccagccc cctttccttg attttctcct tggcccgctg
    28621 cttggtctgg gccgccgcct tttgcttcac agcggacaaa accttgcgga tagagggccg
    28681 ctgctccttg gaaagcgtct tggcggtaaa ctcccgaaac gccagatcaa ggttatctgc
    28741 atccttggcc ttgaaaatca caacgtagtg gggcggctgg gtggtcttat cttttcgtaa
    28801 ggtaaaatcc acgccgtatt tcttggcgca tggcttgaac gctccaatgt tggcctcggt
    28861 gatctcaatg ttggagagat cagccccatg ctttttaagc tgctttaggc tctgctggcc
    28921 gtggtgcagc gtgtttttgc ccttggcccg gtgttgcagg tactttttca gcgccgcttg
    28981 cagcacttga gccgtgagct tgcccgtttt catggacagg gcaatggtct tttgagtaac
    29041 ttcttcctgc aatcgcatcc ctccttgtca gaaagtcaaa agcggcgtct aaggcggaac
    29101 caccagacgc cgcaggtaga tataggtcaa atccgcaccc gcaggccgga aaggtcgtcg
    29161 gcccggatgc cgacaagata ccagttttcg gccatttcaa tgcgggtggg cctccatttg
    29221 ccgccgatca tcacgtcgaa acactccccg caatgcaggc cgccgtaata gtcggccaga
    29281 tcaaagcgga tgtcgtaacg gtctgttcgc tcgtcaaaaa tcaatgcgcc ctgcttcata
    29341 gggtcgtcct ccttttctcg ttacaggctt tcgccctcgc cgcaggaata gtccggcagg
    29401 ccggatttag gctttcccca atcgggctgt gccatgtcgt gcgcgacaag ggccgtgtag
    29461 tagttggaaa tggtgctggg ggcgttgaac agcatagccc gcaggtattg tttgatattg
    29521 cggactttcg ttgtgttctc ccgcagacag tcaaggacaa agcggatatg ttcgccggtc
    29581 agcttcatgt acttggattt caccagctcg gcagggtagt cgtccccggc gacacggatt
    29641 gtctttcggg cggtgcagac ggtttccagc atcaggtcta cgatttcgtc aagctgttca
    29701 cggtcaatgc tcctgtcctg aatgagatag gcatactcga tgttctccaa aatgatttcc
    29761 cgataaatcc gtacagcctc atcctttcct gtccttttcc gttccggcgg cgtagccgct
    29821 gcctcgccct ccataggggc aggggtcggg gatgggatag gaatggaatg ggtactttgt
    29881 ccatctgtaa ttattttttc tttttttgat aagtcagttc tttgtacttc tttatttaat
    29941 tgcgttggat tttccgacgc cggattatcc gatgtcggat tttccaacac cgggttatcc
    30001 gatgttggat tttccaatac cgggttttcc aataccggct gttcgtagat ggtatagacc
    30061 atatcggcca ttttgccttt tgcgtcacgg ccctgctcac gccggatata tccagctttt
    30121 tcaagctccc ataccgctgt cctgatcgcg tcgatgcttt cccggttgat ataggacagt
    30181 ccttttaggg tatagtccca atcctccggc aaggacaaca tctgcgacag caggcccttg
    30241 gcctttaagg tcaggttctt attccgcagg tggtgatttg acatcaccgt atagcctttg
    30301 ttcttttcca cacggaaaac tgccatagcg ttgttcgctc ctttcactgt gaatttgacc
    30361 gctgcatagt cggtcagctt gcccggctga tagggacact ctgcaaactg gcagaattga
    30421 tatttccaat ccgggcggta aaatcggcag gtgccgcaat cctccggcgc gtccgccccg
    30481 ccgccgtcgt agtgatcgaa accgggcttt tcctgcatca gccgttcaaa cgcccggtca
    30541 gggccggaag taaatagcat ggcgtcgcct cctttctcta tttgggcata aaaaagcggc
    30601 gtcctgttct cccatgacgg gaaagaggaa cgccgcgttt acggtgtcgt attcaattta
    30661 tgatgtgccg tatttggggt ggcgggtatt agcagtatgg ttcagcatat atgcggctca
    30721 atccgaaagt agtaaattgg tagtaaattg agttctcata tcctgcgaaa tgcccgtatt
    30781 tccggggctt tcagcgataa cctaaatttt tggaataaaa ctttcattat aggctactgc
    30841 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    30901 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    30961 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    31021 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    31081 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    31141 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    31201 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    31261 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    31321 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    31381 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    31441 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    31501 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    31561 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    31621 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    31681 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    31741 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    31801 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    31861 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    31921 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    31981 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    32041 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    32101 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    32161 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    32221 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    32281 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    32341 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    32401 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    32461 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    32521 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    32581 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    32641 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    32701 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    32761 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    32821 taccagccga aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc
    32881 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    32941 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    33001 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    33061 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    33121 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    33181 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    33241 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    33301 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    33361 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    33421 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    33481 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    33541 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    33601 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    33661 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    33721 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa
    33781 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    33841 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    33901 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    33961 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    34021 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    34081 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    34141 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    34201 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    34261 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    34321 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt
    34381 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    34441 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    34501 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    34561 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    34621 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    34681 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    34741 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    34801 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    34861 gcggtagcgt gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg
    34921 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    34981 gcacatgggc tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg
    35041 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    35101 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    35161 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    35221 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    35281 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    35341 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    35401 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    35461 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    35521 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    35581 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    35641 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    35701 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    35761 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    35821 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    35881 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    35941 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    36001 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    36061 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    36121 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    36181 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    36241 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    36301 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    36361 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    36421 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    36481 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    36541 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    36601 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    36661 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    36721 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    36781 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    36841 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    36901 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    36961 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    37021 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    37081 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    37141 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    37201 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    37261 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    37321 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    37381 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    37441 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    37501 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    37561 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    37621 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    37681 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    37741 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    37801 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    37861 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    37921 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    37981 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    38041 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    38101 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    38161 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    38221 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    38281 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    38341 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    38401 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    38461 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    38521 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    38581 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    38641 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    38701 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    38761 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    38821 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    38881 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    38941 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    39001 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    39061 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    39121 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    39181 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    39241 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    39301 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    39361 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    39421 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    39481 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    39541 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    39601 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    39661 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    39721 aatcagaaac cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca
    39781 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    39841 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    39901 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    39961 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    40021 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    40081 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    40141 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    40201 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    40261 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    40321 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    40381 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    40441 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    40501 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    40561 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    40621 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    40681 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    40741 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    40801 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    40861 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    40921 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    40981 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    41041 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    41101 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    41161 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    41221 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    41281 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    41341 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    41401 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    41461 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    41521 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    41581 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    41641 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    41701 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    41761 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    41821 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    41881 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    41941 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    42001 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    42061 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    42121 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    42181 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    42241 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    42301 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    42361 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    42421 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    42481 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    42541 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    42601 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    42661 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    42721 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    42781 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    42841 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    42901 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    42961 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    43021 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    43081 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    43141 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    43201 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    43261 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    43321 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    43381 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    43441 ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    43501 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    43561 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    43621 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    43681 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    43741 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    43801 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    43861 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    43921 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    43981 ccgtccattt cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc
    44041 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    44101 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    44161 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    44221 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    44281 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    44341 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    44401 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    44461 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    44521 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    44581 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    44641 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    44701 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    44761 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    44821 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    44881 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    44941 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    45001 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    45061 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    45121 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    45181 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    45241 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    45301 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    45361 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    45421 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    45481 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    45541 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    45601 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    45661 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    45721 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    45781 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    45841 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    45901 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    45961 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    46021 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    46081 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    46141 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    46201 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    46261 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    46321 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    46381 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    46441 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    46501 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    46561 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    46621 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    46681 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    46741 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    46801 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    46861 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    46921 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    46981 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    47041 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    47101 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    47161 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    47221 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    47281 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    47341 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    47401 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    47461 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    47521 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    47581 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
    47641 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    47701 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    47761 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    47821 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    47881 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    47941 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    48001 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    48061 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
    48121 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
    48181 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
    48241 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
    48301 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
    48361 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
    48421 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
    48481 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
    48541 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
    48601 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
    48661 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
    48721 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
    48781 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
    48841 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
    48901 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
    48961 gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat
    49021 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
    49081 tttacctttt atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc
    49141 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
    49201 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
    49261 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
    49321 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
    49381 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
    49441 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
    49501 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
    49561 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
    49621 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
    49681 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
    49741 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct
    49801 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
    49861 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
    49921 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
    49981 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
    50041 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
    50101 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
    50161 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
    50221 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
    50281 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
    50341 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
    50401 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
    50461 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
    50521 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
    50581 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
    50641 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
    50701 ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
    50761 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
    50821 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
    50881 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
    50941 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
    51001 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
    51061 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
    51121 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
    51181 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
    51241 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
    51301 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
    51361 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
    51421 tgaatacatt ataatgatac tgtaaatata tctttattat tagtgaatgg atatttactt
    51481 ttaatagata aaatattata actattaaaa ataactattt tcattataat tacaca
//
LOCUS       DAZUMI010000004        49988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000004 DAZUMI010000000
VERSION     DAZUMI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.004"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1722)
                     /locus_tag="ACG81C_000168"
     CDS             complement(22..1722)
                     /locus_tag="ACG81C_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063365.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="ACG81C_000169"
     CDS             2229..3389
                     /locus_tag="ACG81C_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5063366.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="ACG81C_000170"
     CDS             3405..3848
                     /locus_tag="ACG81C_000170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063367.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="ACG81C_000171"
     CDS             complement(4331..4861)
                     /locus_tag="ACG81C_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5063368.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="ACG81C_000172"
     CDS             4966..5517
                     /locus_tag="ACG81C_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5063369.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="ACG81C_000173"
     CDS             complement(5670..6143)
                     /locus_tag="ACG81C_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5063370.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="ACG81C_000174"
     CDS             6281..7753
                     /locus_tag="ACG81C_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5063371.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="ACG81C_000175"
     CDS             7750..8460
                     /locus_tag="ACG81C_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5063372.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8812..9096
                     /locus_tag="ACG81C_000176"
     CDS             8812..9096
                     /locus_tag="ACG81C_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5063373.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9413..9796)
                     /locus_tag="ACG81C_000177"
     CDS             complement(9413..9796)
                     /locus_tag="ACG81C_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5063374.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9798..10382)
                     /locus_tag="ACG81C_000178"
     CDS             complement(9798..10382)
                     /locus_tag="ACG81C_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5063375.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10429..11904)
                     /locus_tag="ACG81C_000179"
     CDS             complement(10429..11904)
                     /locus_tag="ACG81C_000179"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5063376.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12085..12396
                     /locus_tag="ACG81C_000180"
     CDS             12085..12396
                     /locus_tag="ACG81C_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063377.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12511..14847)
                     /locus_tag="ACG81C_000181"
     CDS             complement(12511..14847)
                     /locus_tag="ACG81C_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5063378.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14989..15657
                     /locus_tag="ACG81C_000182"
     CDS             14989..15657
                     /locus_tag="ACG81C_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5063379.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15660..16703
                     /locus_tag="ACG81C_000183"
     CDS             15660..16703
                     /locus_tag="ACG81C_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5063380.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16940..17821)
                     /locus_tag="ACG81C_000184"
     CDS             complement(16940..17821)
                     /locus_tag="ACG81C_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5063381.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17980..18708
                     /locus_tag="ACG81C_000185"
     CDS             17980..18708
                     /locus_tag="ACG81C_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5063382.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18864..19181
                     /locus_tag="ACG81C_000186"
     CDS             18864..19181
                     /locus_tag="ACG81C_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5063383.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19174..19782
                     /locus_tag="ACG81C_000187"
     CDS             19174..19782
                     /locus_tag="ACG81C_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5063384.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19837..20628
                     /locus_tag="ACG81C_000188"
     CDS             19837..20628
                     /locus_tag="ACG81C_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5063385.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20766..21254
                     /locus_tag="ACG81C_000189"
     CDS             20766..21254
                     /locus_tag="ACG81C_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5063386.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21305..22177
                     /locus_tag="ACG81C_000190"
     CDS             21305..22177
                     /locus_tag="ACG81C_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5063387.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22353..22715
                     /locus_tag="ACG81C_000191"
     CDS             22353..22715
                     /locus_tag="ACG81C_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5063388.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22724..23476
                     /locus_tag="ACG81C_000192"
     CDS             22724..23476
                     /locus_tag="ACG81C_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063389.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23490..25292
                     /locus_tag="ACG81C_000193"
     CDS             23490..25292
                     /locus_tag="ACG81C_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063390.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25569..26609
                     /locus_tag="ACG81C_000194"
     CDS             25569..26609
                     /locus_tag="ACG81C_000194"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5063391.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26661..28805)
                     /locus_tag="ACG81C_000195"
     CDS             complement(26661..28805)
                     /locus_tag="ACG81C_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5063392.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDSSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29162..30535)
                     /locus_tag="ACG81C_000196"
     CDS             complement(29162..30535)
                     /locus_tag="ACG81C_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5063393.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG81C_000197"
     CDS             complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG81C_000197"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5063394.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG81C_000198"
     CDS             complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG81C_000198"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5063395.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31983..32996)
                     /locus_tag="ACG81C_000199"
     CDS             complement(31983..32996)
                     /locus_tag="ACG81C_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5063396.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33077..33676)
                     /locus_tag="ACG81C_000200"
     CDS             complement(33077..33676)
                     /locus_tag="ACG81C_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5063397.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33862..34563
                     /locus_tag="ACG81C_000201"
     CDS             33862..34563
                     /locus_tag="ACG81C_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063398.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34573..37284
                     /locus_tag="ACG81C_000202"
     CDS             34573..37284
                     /locus_tag="ACG81C_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5063399.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37735..39363
                     /gene="treC"
                     /locus_tag="ACG81C_000203"
     CDS             37735..39363
                     /gene="treC"
                     /locus_tag="ACG81C_000203"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5063400.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39423..40604)
                     /locus_tag="ACG81C_000204"
     CDS             complement(39423..40604)
                     /locus_tag="ACG81C_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5063401.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40856..42379
                     /locus_tag="ACG81C_000205"
     CDS             40856..42379
                     /locus_tag="ACG81C_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5063402.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42448..43176)
                     /locus_tag="ACG81C_000206"
     CDS             complement(42448..43176)
                     /locus_tag="ACG81C_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5063403.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(43189..44469)
                     /locus_tag="ACG81C_000207"
     CDS             complement(43189..44469)
                     /locus_tag="ACG81C_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5063404.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44610..45212)
                     /locus_tag="ACG81C_000208"
     CDS             complement(44610..45212)
                     /locus_tag="ACG81C_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5063405.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            45457..45663
                     /locus_tag="ACG81C_000209"
     CDS             45457..45663
                     /locus_tag="ACG81C_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063406.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            45975..46694
                     /locus_tag="ACG81C_000210"
     CDS             45975..46694
                     /locus_tag="ACG81C_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5063407.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(46775..47458)
                     /locus_tag="ACG81C_000211"
     CDS             complement(46775..47458)
                     /locus_tag="ACG81C_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5063408.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            47677..49863
                     /locus_tag="ACG81C_000212"
     CDS             47677..49863
                     /locus_tag="ACG81C_000212"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5063409.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        16257 a         8847 c         9971 g        14913 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca
     8521 atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc gtagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct gagtcacaag
     8641 gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt gaccgaggag
     8701 ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt tgtcacagtt
     8761 ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa aatgagggaa
     8821 atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg ggcatatatt
     8881 atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt ccaagcaact
     8941 tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt
     9001 tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg aaaagaaatc
     9061 ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt
     9121 cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     9181 caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg aagcggcttt
     9241 atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg
     9301 gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca taacgctgat
     9361 ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact ggttatttga
     9421 tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct tctgacatcg
     9481 gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg gaagtgatcg
     9541 tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct aagacatcgt
     9601 ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct gtatcacgag
     9661 aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg atcaataagt
     9721 ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt acttcaacta
     9781 tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt tacaatttca
     9841 aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt ttgaggctga
     9901 ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag
     9961 ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa ctttcgttgc
    10021 tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc aacagagata
    10081 cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa aaatgatccg
    10141 ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag gctttcgaat
    10201 tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg actgatgatt
    10261 ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat ggcataaatg
    10321 gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc caattgttcc
    10381 attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt atttcaaata
    10441 tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt tcatatcgaa
    10501 aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa tagagccagg
    10561 attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg ggatcacgat
    10621 gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa
    10681 aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc
    10741 aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac ggatttgcat
    10801 gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta cttttcctgg
    10861 aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg ccatagatac
    10921 gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc ggtgagtaac
    10981 tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc caggaaattc
    11041 tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat ttggcatgat
    11101 ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg ctataccatc
    11161 aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat tgtagccaag
    11221 tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta aaccaatatt
    11281 gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg
    11341 gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa ccatacgatg
    11401 gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac ccattgtgat
    11461 gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa
    11521 gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt
    11581 cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc
    11641 atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt tcacacgtgt
    11701 gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag tcggacgttt
    11761 tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt caggatcgat
    11821 ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga tgacttttct
    11881 taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta cctatctata
    11941 gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc tacaatggaa
    12001 tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa aagaaagcgc
    12061 atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc taaagcagta
    12121 aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga agtaaaatac
    12181 gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa aaaaggaaga
    12241 ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac tgaagtaacg
    12301 attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc agaagagttg
    12361 tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa aaacagcgcg
    12421 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    12481 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    12541 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    12601 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    12661 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    12721 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    12781 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    12841 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    12901 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    12961 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    13021 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    13081 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    13141 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    13201 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    13261 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    13321 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    13381 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    13441 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    13501 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    13561 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    13621 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    13681 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    13741 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    13801 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    13861 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    13921 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    13981 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    14041 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    14101 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    14161 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    14221 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    14281 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    14341 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    14401 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    14461 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    14521 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    14581 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    14641 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    14701 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    14761 tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata
    14821 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    14881 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    14941 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    15001 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    15061 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    15121 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    15181 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    15241 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    15301 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    15361 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    15421 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    15481 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    15541 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    15601 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    15661 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    15721 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    15781 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    15841 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    15901 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    15961 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    16021 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    16081 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    16141 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    16201 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    16261 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    16321 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    16381 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    16441 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    16501 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    16561 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    16621 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    16681 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    16741 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    16801 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    16861 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    16921 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    16981 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    17041 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    17101 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    17161 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    17221 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    17281 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    17341 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    17401 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    17461 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    17521 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    17581 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    17641 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    17701 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    17761 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    17821 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    17881 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    17941 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    18001 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    18061 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    18121 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    18181 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    18241 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    18301 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    18361 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    18421 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    18481 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    18541 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    18601 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    18661 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    18721 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    18781 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    18841 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    18901 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    18961 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    19021 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    19081 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    19141 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    19201 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    19261 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    19321 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    19381 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    19441 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    19501 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    19561 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    19621 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    19681 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    19741 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    19801 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    19861 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    19921 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    19981 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    20041 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    20101 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    20161 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    20221 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    20281 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    20341 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    20401 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    20461 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    20521 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    20581 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    20641 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    20701 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    20761 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    20821 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    20881 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    20941 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    21001 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    21061 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    21121 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    21181 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    21241 gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag
    21301 tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt
    21361 cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg
    21421 ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac
    21481 tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat
    21541 tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa
    21601 agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga
    21661 aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc
    21721 gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca
    21781 atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt
    21841 gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat
    21901 cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg
    21961 tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat
    22021 cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg
    22081 gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc
    22141 tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag
    22201 aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga
    22261 caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa
    22321 tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg
    22381 ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag
    22441 aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg
    22501 gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa
    22561 aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa
    22621 tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc
    22681 cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga
    22741 agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag
    22801 cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc
    22861 tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag atgggcatat
    22921 ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc
    22981 gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga
    23041 aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt
    23101 ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat
    23161 ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat
    23221 tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga
    23281 tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc
    23341 gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga
    23401 agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg
    23461 aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt actgggatca
    23521 agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt
    23581 tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg
    23641 cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt
    23701 atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta
    23761 tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag
    23821 tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc
    23881 accgattatt agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc
    23941 acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca
    24001 atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa
    24061 caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc
    24121 taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg
    24181 tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct
    24241 ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat
    24301 tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc
    24361 tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag
    24421 acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc
    24481 agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg
    24541 cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac
    24601 atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg
    24661 aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa
    24721 tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc
    24781 tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa
    24841 ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt
    24901 atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag
    24961 ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg
    25021 acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat
    25081 cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg
    25141 tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg
    25201 ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat
    25261 atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga
    25321 acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg
    25381 caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc
    25441 tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag
    25501 ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg
    25561 caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca
    25621 cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc
    25681 aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat
    25741 atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga cggattacga
    25801 atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc
    25861 attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca
    25921 atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat
    25981 ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag
    26041 tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt
    26101 tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc
    26161 gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg
    26221 gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc
    26281 gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt
    26341 tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag
    26401 atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg
    26461 ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg
    26521 agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa
    26581 cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca
    26641 catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct
    26701 aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct
    26761 ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg
    26821 gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca
    26881 tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    26941 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    27001 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    27061 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    27121 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    27181 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    27241 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    27301 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    27361 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    27421 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagctatc taatccatct
    27481 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    27541 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    27601 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    27661 gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta
    27721 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    27781 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    27841 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    27901 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    27961 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    28021 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    28081 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    28141 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    28201 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    28261 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    28321 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    28381 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    28441 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    28501 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    28561 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    28621 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    28681 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    28741 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    28801 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    28861 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    28921 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    28981 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    29041 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    29101 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    29161 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    29221 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    29281 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    29341 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    29401 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    29461 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    29521 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    29581 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    29641 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    29701 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    29761 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    29821 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    29881 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    29941 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    30001 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    30061 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    30121 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    30181 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    30241 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    30301 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    30361 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    30421 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    30481 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    30541 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    30601 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    30661 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    30721 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    30781 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    30841 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    30901 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    30961 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    31021 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    31081 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    31141 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    31201 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    31261 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    31321 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    31381 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    31441 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    31501 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    31561 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    31621 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    31681 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    31741 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    31801 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    31861 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    31921 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    31981 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    32041 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    32101 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    32161 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    32221 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    32281 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    32341 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    32401 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    32461 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    32521 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    32581 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    32641 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    32701 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    32761 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    32821 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    32881 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    32941 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    33001 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    33061 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    33121 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    33181 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    33241 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    33301 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    33361 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    33421 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    33481 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    33541 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    33601 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    33661 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    33721 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    33781 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    33841 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    33901 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    33961 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    34021 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    34081 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    34141 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    34201 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    34261 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    34321 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    34381 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    34441 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    34501 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    34561 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    34621 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    34681 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    34741 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    34801 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    34861 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    34921 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    34981 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    35041 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    35101 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    35161 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    35221 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    35281 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    35341 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    35401 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    35461 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    35521 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    35581 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    35641 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    35701 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    35761 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    35821 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    35881 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    35941 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    36001 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    36061 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    36121 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    36181 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    36241 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    36301 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    36361 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    36421 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    36481 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    36541 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    36601 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    36661 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    36721 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    36781 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    36841 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    36901 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    36961 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    37021 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    37081 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    37141 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    37201 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    37261 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt
    37321 attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg
    37381 aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc
    37441 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    37501 gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa
    37561 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    37621 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    37681 ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    37741 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    37801 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    37861 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    37921 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    37981 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    38041 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    38101 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    38161 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    38221 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    38281 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    38341 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    38401 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    38461 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    38521 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    38581 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    38641 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    38701 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    38761 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    38821 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    38881 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    38941 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    39001 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    39061 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    39121 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    39181 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    39241 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    39301 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    39361 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    39421 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
    39481 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    39541 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    39601 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    39661 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    39721 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    39781 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    39841 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    39901 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    39961 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    40021 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    40081 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    40141 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    40201 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    40261 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
    40321 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    40381 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    40441 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    40501 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    40561 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
    40621 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    40681 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
    40741 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
    40801 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    40861 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    40921 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    40981 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    41041 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    41101 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    41161 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    41221 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
    41281 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
    41341 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    41401 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    41461 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
    41521 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
    41581 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    41641 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
    41701 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    41761 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
    41821 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
    41881 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
    41941 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    42001 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    42061 gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt
    42121 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    42181 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    42241 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
    42301 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    42361 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    42421 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    42481 ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg
    42541 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    42601 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    42661 tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    42721 gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc
    42781 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt
    42841 gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg
    42901 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    42961 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    43021 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    43081 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    43141 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    43201 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    43261 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    43321 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
    43381 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    43441 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    43501 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
    43561 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    43621 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    43681 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    43741 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    43801 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    43861 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    43921 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    43981 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    44041 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
    44101 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
    44161 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    44221 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    44281 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    44341 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    44401 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    44461 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
    44521 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    44581 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    44641 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    44701 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
    44761 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    44821 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    44881 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    44941 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    45001 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    45061 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    45121 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    45181 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    45241 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    45301 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    45361 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
    45421 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
    45481 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
    45541 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
    45601 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
    45661 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
    45721 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
    45781 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    45841 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
    45901 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    45961 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    46021 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    46081 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    46141 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    46201 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    46261 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    46321 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    46381 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    46441 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    46501 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    46561 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    46621 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    46681 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    46741 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    46801 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    46861 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    46921 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    46981 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    47041 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    47101 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    47161 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    47221 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    47281 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    47341 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    47401 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    47461 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    47521 aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg
    47581 tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca
    47641 aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga
    47701 ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg
    47761 ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt
    47821 gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag
    47881 aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag
    47941 aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa
    48001 gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt
    48061 acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc
    48121 gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga
    48181 aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa
    48241 aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa
    48301 tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta
    48361 gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag
    48421 ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag
    48481 caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag
    48541 atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact
    48601 aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt
    48661 gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc
    48721 aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct
    48781 cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata
    48841 ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct
    48901 gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat
    48961 gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc
    49021 caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt
    49081 atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca
    49141 ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac
    49201 tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc
    49261 gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga
    49321 gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa
    49381 agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata
    49441 cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc
    49501 caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat
    49561 gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg
    49621 attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac
    49681 tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat
    49741 gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca
    49801 agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag
    49861 tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga
    49921 aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa
    49981 cacagttt
//
LOCUS       DAZUMI010000005        46346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000005 DAZUMI010000000
VERSION     DAZUMI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.005"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..910
                     /locus_tag="ACG81C_000213"
     CDS             <1..910
                     /locus_tag="ACG81C_000213"
                     /inference="COORDINATES: protein motif:HMM:NF016309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFS5063410.1"
                     /translation="QNYNTNSYNRKIENLKNNQQKIKHFYAYILEFLDTPVMKNISSR
                     RPINVPKSLVMNPKYNTIYKMYLKSRNNRNTLNFSNRYQYYWKSTDVLYEIWCFIKII
                     RSLSLQGYTPISGWIFENDILSQELPFLHEDTTVVLSKNDIRINLVYNNSMEKEYFSN
                     TLDIPVKTSSKRNKPDIRLDIILQDKYYIGSLIFEVKYKKLDNILDNDKGRQRQQLMA
                     YKQNTMSSILAFPEILTRSLQAVSAVFALYPSNEGRKKTAPKYYEKEGIFFHLLNPSS
                     DEKELSVKIQTSIEERISLFNQQSRN"
     gene            complement(1100..1579)
                     /gene="rlmH"
                     /locus_tag="ACG81C_000214"
     CDS             complement(1100..1579)
                     /gene="rlmH"
                     /locus_tag="ACG81C_000214"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5063411.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2007..3296
                     /locus_tag="ACG81C_000215"
     CDS             2007..3296
                     /locus_tag="ACG81C_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS5063412.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3335..3940)
                     /gene="ytpR"
                     /locus_tag="ACG81C_000216"
     CDS             complement(3335..3940)
                     /gene="ytpR"
                     /locus_tag="ACG81C_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5063413.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4145..4609)
                     /locus_tag="ACG81C_000217"
     CDS             complement(4145..4609)
                     /locus_tag="ACG81C_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5063414.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4631..4951)
                     /locus_tag="ACG81C_000218"
     CDS             complement(4631..4951)
                     /locus_tag="ACG81C_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5063415.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5095..6174)
                     /gene="pepA"
                     /locus_tag="ACG81C_000219"
     CDS             complement(5095..6174)
                     /gene="pepA"
                     /locus_tag="ACG81C_000219"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5063416.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6374..6724
                     /locus_tag="ACG81C_000220"
     CDS             6374..6724
                     /locus_tag="ACG81C_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5063417.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6947..7894
                     /locus_tag="ACG81C_000221"
     CDS             6947..7894
                     /locus_tag="ACG81C_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5063418.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7951..9369)
                     /gene="pepV"
                     /locus_tag="ACG81C_000222"
     CDS             complement(7951..9369)
                     /gene="pepV"
                     /locus_tag="ACG81C_000222"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5063419.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            9518..10363
                     /locus_tag="ACG81C_000223"
     CDS             9518..10363
                     /locus_tag="ACG81C_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5063420.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10367..10807
                     /locus_tag="ACG81C_000224"
     CDS             10367..10807
                     /locus_tag="ACG81C_000224"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5063421.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10857..11051
                     /locus_tag="ACG81C_000225"
     CDS             10857..11051
                     /locus_tag="ACG81C_000225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063422.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11187..11800
                     /locus_tag="ACG81C_000226"
                     /pseudo
     CDS             11187..11800
                     /locus_tag="ACG81C_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(12208..12897)
                     /locus_tag="ACG81C_000227"
     CDS             complement(12208..12897)
                     /locus_tag="ACG81C_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063423.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12872..15001)
                     /locus_tag="ACG81C_000228"
     CDS             complement(12872..15001)
                     /locus_tag="ACG81C_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS5063424.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15049..16386)
                     /locus_tag="ACG81C_000229"
     CDS             complement(15049..16386)
                     /locus_tag="ACG81C_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5063425.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16638..16997)
                     /gene="rplT"
                     /locus_tag="ACG81C_000230"
     CDS             complement(16638..16997)
                     /gene="rplT"
                     /locus_tag="ACG81C_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5063426.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17050..17250)
                     /gene="rpmI"
                     /locus_tag="ACG81C_000231"
     CDS             complement(17050..17250)
                     /gene="rpmI"
                     /locus_tag="ACG81C_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5063427.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(17322..17846)
                     /gene="infC"
                     /locus_tag="ACG81C_000232"
     CDS             complement(17322..17846)
                     /gene="infC"
                     /locus_tag="ACG81C_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5063428.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17890..18015)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            18166..18798
                     /locus_tag="ACG81C_000233"
     CDS             18166..18798
                     /locus_tag="ACG81C_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5063429.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18800..19705)
                     /locus_tag="ACG81C_000234"
     CDS             complement(18800..19705)
                     /locus_tag="ACG81C_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5063430.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19790..20449)
                     /locus_tag="ACG81C_000235"
     CDS             complement(19790..20449)
                     /locus_tag="ACG81C_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5063431.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            20765..21709
                     /gene="mvk"
                     /locus_tag="ACG81C_000236"
     CDS             20765..21709
                     /gene="mvk"
                     /locus_tag="ACG81C_000236"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5063432.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21702..22679
                     /gene="mvaD"
                     /locus_tag="ACG81C_000237"
     CDS             21702..22679
                     /gene="mvaD"
                     /locus_tag="ACG81C_000237"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5063433.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22692..23777
                     /locus_tag="ACG81C_000238"
     CDS             22692..23777
                     /locus_tag="ACG81C_000238"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5063434.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23774..24838
                     /gene="fni"
                     /locus_tag="ACG81C_000239"
     CDS             23774..24838
                     /gene="fni"
                     /locus_tag="ACG81C_000239"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5063435.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25087..25890)
                     /locus_tag="ACG81C_000240"
     CDS             complement(25087..25890)
                     /locus_tag="ACG81C_000240"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5063436.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25910..26752)
                     /gene="kduI"
                     /locus_tag="ACG81C_000241"
     CDS             complement(25910..26752)
                     /gene="kduI"
                     /locus_tag="ACG81C_000241"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS5063437.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26768..27775)
                     /locus_tag="ACG81C_000242"
     CDS             complement(26768..27775)
                     /locus_tag="ACG81C_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5063438.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27782..28426)
                     /locus_tag="ACG81C_000243"
     CDS             complement(27782..28426)
                     /locus_tag="ACG81C_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5063439.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(28526..29284)
                     /locus_tag="ACG81C_000244"
     CDS             complement(28526..29284)
                     /locus_tag="ACG81C_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS5063440.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(29411..30562)
                     /gene="fucO"
                     /locus_tag="ACG81C_000245"
     CDS             complement(29411..30562)
                     /gene="fucO"
                     /locus_tag="ACG81C_000245"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS5063441.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30644..30955)
                     /gene="rhaM"
                     /locus_tag="ACG81C_000246"
     CDS             complement(30644..30955)
                     /gene="rhaM"
                     /locus_tag="ACG81C_000246"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS5063442.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30957..31802)
                     /gene="rhaD"
                     /locus_tag="ACG81C_000247"
     CDS             complement(30957..31802)
                     /gene="rhaD"
                     /locus_tag="ACG81C_000247"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS5063443.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31816..33072)
                     /gene="rhaA"
                     /locus_tag="ACG81C_000248"
     CDS             complement(31816..33072)
                     /gene="rhaA"
                     /locus_tag="ACG81C_000248"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS5063444.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33069..34529)
                     /gene="rhaB"
                     /locus_tag="ACG81C_000249"
     CDS             complement(33069..34529)
                     /gene="rhaB"
                     /locus_tag="ACG81C_000249"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS5063445.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34682..35551
                     /locus_tag="ACG81C_000250"
     CDS             34682..35551
                     /locus_tag="ACG81C_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5063446.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35677..36576)
                     /locus_tag="ACG81C_000251"
     CDS             complement(35677..36576)
                     /locus_tag="ACG81C_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063447.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36741..37565)
                     /locus_tag="ACG81C_000252"
     CDS             complement(36741..37565)
                     /locus_tag="ACG81C_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5063448.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(37576..38871)
                     /locus_tag="ACG81C_000253"
     CDS             complement(37576..38871)
                     /locus_tag="ACG81C_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS5063449.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38887..39366)
                     /locus_tag="ACG81C_000254"
     CDS             complement(38887..39366)
                     /locus_tag="ACG81C_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5063450.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39372..40400)
                     /locus_tag="ACG81C_000255"
     CDS             complement(39372..40400)
                     /locus_tag="ACG81C_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS5063451.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(40417..41523)
                     /locus_tag="ACG81C_000256"
     CDS             complement(40417..41523)
                     /locus_tag="ACG81C_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5063452.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41752..44349)
                     /gene="adhE"
                     /locus_tag="ACG81C_000257"
                     /gene_synonym="adhC"
     CDS             complement(41752..44349)
                     /gene="adhE"
                     /locus_tag="ACG81C_000257"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5063453.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44654..45034)
                     /gene="yajC"
                     /locus_tag="ACG81C_000258"
     CDS             complement(44654..45034)
                     /gene="yajC"
                     /locus_tag="ACG81C_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5063454.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(45131..46276)
                     /gene="tgt"
                     /locus_tag="ACG81C_000259"
     CDS             complement(45131..46276)
                     /gene="tgt"
                     /locus_tag="ACG81C_000259"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5063455.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
BASE COUNT        13386 a         9861 c         8374 g        14725 t
ORIGIN      
        1 acaaaactac aatacaaata gctataatag aaagattgaa aatttgaaaa acaatcagca
       61 aaagataaaa catttttatg cttatatcct tgaattttta gacacaccag taatgaaaaa
      121 catttcatca agaagaccta taaatgttcc aaaatcactt gttatgaacc ctaaatataa
      181 cactatatat aaaatgtatt taaaaagtag aaacaatcgt aatactttaa atttttcaaa
      241 tagatatcag tattattgga aaagtacaga tgtactctat gaaatttggt gctttataaa
      301 aattattaga tctctatccc tgcaaggcta cactccaatt tctggatgga tttttgagaa
      361 tgatattctt tcacaagaat tgccgttttt acatgaagac actaccgtag tattgagtaa
      421 aaatgatata cgtattaatc ttgtatataa taattccatg gaaaaagaat atttctccaa
      481 tacactagat atcccagtaa aaacctcttc aaaacgaaat aaacctgata ttagattaga
      541 cattatcctt caagataaat actatatcgg aagcttaata tttgaagtaa aatataaaaa
      601 actagacaat atacttgata atgataaagg taggcaacgt caacaattaa tggcttataa
      661 acaaaatact atgtcaagta ttctagcatt tcctgaaatt cttacacgtt ctttgcaagc
      721 tgtctctgct gtctttgctt tatatccttc caatgaagga agaaaaaaaa ctgcacctaa
      781 atattatgaa aaagaaggta ttttttttca cttattaaat ccttcatcag atgaaaaaga
      841 gctttcagta aaaattcaaa caagtattga agaaagaatt tcattattta atcaacaaag
      901 tagaaattaa aaagatgaag agcgtctctt tatacaagag agcgctcttc atcttcaatt
      961 ttttgtaaca aatttgaata cataataaag ttattattat ttttttgaaa ttctcaacga
     1021 ttttcaagtt aactttttta tatataaatt tcaatgaaaa gctaacttag tgttcaggag
     1081 cattcaatct taaatatcat tatttatgat acggagcccc cgcattgatc cggaaggcac
     1141 ggtagatttg ctcgacaagg atcagacgca tcaattgatg tggatacgtc atttttccaa
     1201 aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa gagccgccga
     1261 taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt ttagcaaaag
     1321 attcacttgt aggatttttc ccttcaattg ctaaggcata gacgtattct tgttctttga
     1381 ttttcgctaa gatccgttcc ccttcttttt cttttacttg tctcatttgc gcttcgctta
     1441 aattctccgg ggctttttcg tcaggaactt cgatcaattc gatttttgca taggcattca
     1501 gccgtttgac atactcattg ataccttgga tcaggtattt ttcttttaat tttcctactg
     1561 agataatttt gatattcatg ttttctctcc tttggtcttc atgctcactt tgtttattat
     1621 aatcaatttt ttcgtctata tgtttatttt ggctattttt gttaaaaaga tttccccaac
     1681 agttatgcac aacatccaca ggtttatcca cagaaaacag tgttatttta ccttatttca
     1741 tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata cacaggctgt
     1801 ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac agtatctgtg
     1861 gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag tttttcataa
     1921 aaagttcaca gtctcaaagt agactaaagt caacaaataa gactatacta acagtagata
     1981 aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca aagatgaaaa
     2041 aaagtaaaaa taatagcctc tggcgtaagt taggtctggg gcttgttgga gggatcgttg
     2101 gaggactact gacagctggc attttttatg cagcgatggg cactggcaac aatagttcta
     2161 ctgctacaag cggcaatcag aattctgccg gtgaaacagt tgtggaaaat gtcaaagtca
     2221 atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc gtttctgtta
     2281 tcaacttaca aagtcaaaac caatcaagcg gttttggcgg actattcggg cagcaagaag
     2341 aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc ggcgtgatct
     2401 acaaaaaaga cgggaatact gcttatgtag tcacgaataa tcacgtagta gatggccagc
     2461 aaggtcttga agtcttgatg aaagacggta cgaaggtaaa agctgaatta gttggaacag
     2521 atgcttattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa acagttgcct
     2581 cttttggtga ctcaagtgct ctgaaggttg gtgagcctgc gattgcgatt ggttccccac
     2641 taggatctga atatgcaaat tctgtcactt caggaatcat ttcttccttg aaccgacaag
     2701 tgacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa acagatgcag
     2761 caatcaaccc tggaaattct ggtggtcctt tagttaatat cgaaggacaa gttatcggta
     2821 tcaactcaag taagatcgca agtacttctg aatctacttc taatgtaagc gtagaaggaa
     2881 tgggatttgc tattccaagt aacgatgtag tgaatatcat caatcaactg gaaaaagacg
     2941 gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc gtttctactc
     3001 agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc atcgtgacaa
     3061 atgtccaaac agctacaccg gctgaaaaag ctggattgaa acaatacgac gtcattacta
     3121 agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg tataagaaaa
     3181 aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa acaactactg
     3241 ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa aattaataaa
     3301 aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatgct tctccgacta
     3361 cggcatcctc aggcaatact aagattcctt tttttgctgg agcatttggc aaatgcaatt
     3421 cacgagcgga acagatcatt ccatagctat ctacccctcg caaagttcct ggccagatca
     3481 tcattccatc tggcatcata gctcctggtt ttgcaacgac aactttttgt ccagcttcga
     3541 tattgggtgc accgcaaacg atttgcaata ctgtaccatt gtctacttct gtctcagtaa
     3601 tcgataaatg gtcagaatca ggatgtggga cgcatgtttt tacgtacccg acaacgatct
     3661 tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttgatc aatcgattca
     3721 atttcgctaa ttcctcatct gttaagctta cttggccgtt cccttcgatt gttagatgat
     3781 cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg atccgagcaa
     3841 cattccattt gcgttcaaca gtgttttctg ttcctttatc gtcggctata ataaccatca
     3901 aggtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct ccattatcct
     3961 ttgctgagat ttatcttaac aaaaaattgc attcactgca tgaaattttt ttcatgcttt
     4021 atttccgaag aagcctcttc tggaacacca tttattcgta acaagggact gtgacaagac
     4081 ttttgtcaca gtccctttac tgttttagtt aattgattaa aaaggtcgta caagttccac
     4141 ttgcttaatt acttggctgt tcttcttcgt cagaaacgat caagacatca caaggtgaat
     4201 gacgtatcac gtagctggct acacttcctg tcataaagcg ttcgactgca tttagacctg
     4261 actgcccaac catgattaga tcgatctcat atttttttgg caatttgact gccatggatt
     4321 cacgaacaga accatatgca ataattcctt ctatatttgt gtaatcaaca gatttcccat
     4381 attctttaca ttcttcgatc atttccttgg ctgtttcagt ttgctgatcg actagatttt
     4441 gatcaatagg tgcatagccc ataaagttat aaaattgctg atcgattacg tttgctacat
     4501 atactttccc attattacgg cgagccactt ctaccgcttt tttgaaagca gcaagtgctt
     4561 gttcactacc gtcgatacct actaaaatat tcttatattc ttgattcatc ttgacccctc
     4621 ctaacagatg tcagcctttt gttacacttt ctaggaacga ttcgatttcc tgttttgttt
     4681 tacgatcttt attcactaga cgacctaatt cttttccgtc ttggatcacg acaaaacttg
     4741 ggataccaaa aatgttccat tcagcggcta catctatgta cttgtcacgg tctacttcaa
     4801 taaattgcca atcagaaaag tcattttcaa tttcaggcat ttgcggtttg ataaaacggc
     4861 agtctccgca ccagccggca gtaaagaaaa atacactctt tccagtttct acgtagcctg
     4921 ctaattcttc taaactattt gggataatca ttctttgtcc tctactttct gtttatgtat
     4981 ttttcaatta aattatacac ctttctttcg aactaacaaa cattgatcct ctttttataa
     5041 aaaaaatctg gcaaaaagca atcttttgcc agattttata gtttcactat cgcattattt
     5101 tccgtatacg atcgtattca ctgttgattt atccaaacct ttcagtgttt gaatcaacat
     5161 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     5221 tcccactacg ccaatcactg tacttgggat tccttcgttt tgtgtatgag cagcacctgc
     5281 atctgtaccg ccttttgaaa caaagtattg ataagggata ttgtttgttt cagcgatatc
     5341 cagcaagtac tcgcgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     5401 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtatcat ctgctgcaga
     5461 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     5521 caagcctact tcttcctgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     5581 ttgcaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccatgattt
     5641 actgataata ttctttccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     5701 tcctggaagg acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     5761 aatatcggtt acttctactg atttttgtcc gcttgttcct cgcaataaat gtgggggaac
     5821 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgctgagc
     5881 agaaacaacg taaggattcc agccgcctag aggtacgact ttaaaaagcc cgttttcttg
     5941 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacggggtgc
     6001 atccgcttct tcatgatggc gcagaccaaa aatgccgccc aaaccatcta actgtaactc
     6061 atctaccagc ggttccatat gactttccat ataagcgcga acatcttgtt caaaaccgct
     6121 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     6181 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     6241 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     6301 tttcaaagaa aatatgagag tgtggtaaaa taagcacaag gagtaacaag tgagaagaag
     6361 gaggtatgtc cacatgtatg aagaaagcga actaagctat tttaaaggcg ggttagcagt
     6421 tggaatcggt ctaggattat tgagcggggt agcttcaacg atttggtatc atcgcaaaaa
     6481 agtattgtca gcagaccaag tacttgaaaa tatcaagtca tcttttctaa aagaaggtgc
     6541 aatcgaaggt tcttggatct cttttgaaaa ggaacctatt aggaaattcg ccgttcggtc
     6601 gagtgcttat cggggaggtc tttctcgaat agaagatggc gaaactgttt attatgaatt
     6661 tttagcagat gctcatacag gaaccgtatt agaaatttca agaaaaaaag gtgcaacaga
     6721 ttaatacgtc aaaaaagtga gagaacgaat acgcacccac tattcaaaag agaggtatca
     6781 tgtccttctt ttgtagtggg tggtttttta gatagagctt tccaaacata tattccagta
     6841 ttttcttttt aaataaatga tatttagcgg gaaaaactag catttccttt gattatgagg
     6901 tatttttaat ttagcttttg ttttctatag ttaaggagag tgaaaaatgg aacaacaaga
     6961 aaaaattgct gttctgattc cttgttacaa tgaggaagca acaatttcaa cagtcattgc
     7021 agactttaaa cgagaattac cagaagcaga tatttatgtc tatgataata attcgaccga
     7081 tcgaacatac gaattggccg ttcaaggtgg agcaatcgta aaaaaagaac ctcgtcaagg
     7141 aaaaggaaat gtgatccgcc aaatgttctt tgatatcgat gccgactatt atttaatggt
     7201 agatggcgat gatacttatc ctgcagaagc tgtacatggt ttgctcgaga aattacgttc
     7261 tggtgaagca gatatggtca ttggtgatcg cctatcaaat ggaacctact ttgatgagaa
     7321 taagagacct ttccacgatt ttggaaataa ccttgttaga aatacaatta ctagaatgta
     7381 taaaacaaaa attcttgatg tcatgaccgg ctatcgagga ttcaatcgta tctttgtcaa
     7441 aagttttcct attatgagtt ctggttttca aatcgaaacg gaattgacga tccatgcttt
     7501 agataaaaaa ttcaagttcg ttgaaatccc aatcgattat cgtgatcgtc ctgaaggcag
     7561 cgaatcaaaa ttgaatacat tctctgacgg attcaaagtg atcatgatga ttattaaaat
     7621 gtttaaagat tataagcctt tgcttttctt tgggatttgg accatcttct tctttttatt
     7681 tggattggtc acggggattc ctgtgattcg cgaatatatg ctgacaagct ttattacacg
     7741 catcccttct gcgattttaa gtactggatt gatgacttta gcacttcttt cattagtaac
     7801 agggttgatt ttggatacag tcgtcacgaa cgctaagaaa gaatatgaat tgaatctata
     7861 tcatgtttat catgaatacc acagtagaaa gtaaaaaaag gatcaagact gcaaaaataa
     7921 tttgcaattc ttgatcctct ttttaattaa ttatttcgcc aaacgataga tggcatctgc
     7981 atagattgct gttgctctga acaagtcatc aagactcata aattcgtttg cttgatgcat
     8041 tgtatcagta tatcctggga acatcgctcc ataagcaacg ccacgtttca acaaacgtcc
     8101 gtaagttccg ccaccgatga tttgttcgta gcctttttct ccagtgtggt cttcatagac
     8161 ttgcaataat gtagccacca atggatcatc cattggtacg taatgtggtt ccattacgcg
     8221 agcacctcga gtaactgttg cgccttcttg ccccactgtt tgttcgatgt cactttgcaa
     8281 tccgtctact gttacacctt ttggaaaacg gaaattcata ttgatgaagt tcgcttcctc
     8341 tgtaccattt tcttcaaaag caaatagtcc agcattcatt gtcaatttgc ccattttttc
     8401 atcttcataa gcgactccta gtttttctcc ataaaaatct tcatggacat aagcagctgc
     8461 tacatggata aattgtttag cagatcctag gaattcatag ttatctaaga acaatgccaa
     8521 gaaagaacct gcattgatac cagattgtgg gcttgcacca tgtgcgcctt taccaactaa
     8581 ttcgattttt acatacgtat tgtccgcttc aatcgttccg cttactggat ttgcttcgat
     8641 gaattggtaa aatgcttctt ccatcgcgat agctgcatct gcacttggta cttgaagtgc
     8701 tgcagttgct gttcctggca ccatattttc gcgaagtccg gaaacaaaag atttcaagac
     8761 ataatctcct gcattgtcgc ctttgaagcg taaagctaaa gaaacatttc ctttttcacc
     8821 attgatgatt gggaattcag catctggtga gaaaccaaaa tctggttctt cttcatgttc
     8881 gaaataataa gccatatcgc cccagccgct ttcttcatcg ctgccgacta cgaagcgaac
     8941 tttttttgat agtttcaagt caagttcttt aatgatcttc atcgcatagt aagctgccat
     9001 acttggacct ttgtcatcgc ttgatccgcg tgcaaagatc tttccatcct tgattactgg
     9061 ttcataagga tctgtgtccc agccatctcc tgcaggaacg acatccatgt gtccaaatat
     9121 ccctagcgtt tcgtctcctt cgccaagatc aatatgccct gcatagttat cgacattttt
     9181 tactttaaat ccatcacgtt cgccataagc taacatatgt ttcaatgcgt cgcgaggtcc
     9241 aggtccaaat ggtgcatctg gtgttacttt tgagtcatcg cgctcgctgt tgacacgtaa
     9301 aagatttttc aagtcttcaa gcaagtcttc tttacgtgct tctacttctt tttgccaatc
     9361 aattgtcata aagttaatcc tccacttatt taagtcttcg tgttcatgat accacaaatt
     9421 acagaaaaaa atagttcaaa aaatctgaca attccttttc ttatctgttt ttctctaaaa
     9481 aaaatatacg ctataatgag taaaaggagg tttttagatg ctgataaacg aacaactagt
     9541 gaaaagagtc atcacacctc gtccaaaaat ttcacataaa ggaacatttg gacgtatcgt
     9601 tttgattggc ggcaatgaac aatatggcgg agcaatcatt atgagtgctt tagctgccgt
     9661 acacagcgga gcgggtctca cgactgtagt aactgatgaa aaaaatcgga gtgctcttca
     9721 cagtcatctg ccagaagcaa tgattgtagt ttggacggat actgaccagt ttctttccgt
     9781 aataaagcaa gcagatgtac tcttgattgg tcccggattg ggtacttctt ctaagagttt
     9841 ggaacgtttc aaacaagtac tagaaaacca atcaaaagaa caatggctgg tgattgatgg
     9901 ctctgcttta agtttacttg ctgctaatca tcttttgcta ccctatcccg aacaaactgt
     9961 acttacacct catcaaatgg agtggcaacg gttgagttct ctaccgatac aagaacaacg
    10021 ggataaagaa aacaaaaaga agcaacagga aatcggtgct gtgctcgttt tgaaaagtca
    10081 tgagacaaaa atttatggtc accagcactt ctacaccaat cctttaggtt ctgctgctat
    10141 ggcaacgggc ggcatgggtg acactttagc tgggatgatc acaggatttt tagcccagtt
    10201 tcctcaaaat gaccaaacga ttgcagcagc tatttatctt catagtttca ttggagacca
    10261 gctggcaaaa gaacaatata ttgttttgcc gacacaaatc agcgaagcac ttccgaaatg
    10321 gatgaattat ttttgtaaaa aatcaatgga aaacgaggca tgagtcatgg agaaatttac
    10381 aggttatgtg cgagaacctt tttattatga aacagatcaa atggggatta ttcatcacag
    10441 caattatatc cgctggtttg aagaggctcg ggtagcctta ttggtacatc tagggtttcc
    10501 ctataaaaaa atcgaagaat ctgggatcat catccccgtt ctggaagtaa attgcagcta
    10561 taaagaaatg atccattacg gagaaaaagt gagcatccat ccaactatcc aaaagtatac
    10621 ggggacacgg ttggatttta gctacgaaat tgtgggaact gaagacaaaa aattaaaaac
    10681 tactggcagt agtaaacact gttttttact ttctgattcg caacgattag tgaagctgag
    10741 caaagtcaat ccggagttag atcagttatt ccaagcgtac gcttctttgg atgatgtaaa
    10801 ttcttaataa agaccgtcta agcatttttc ttgtattgtg tcgagtagtt cgtataatga
    10861 aaagaaaaaa gcaggtgatc cccatgtatg tcgttaaagt aatgcacggt tacatcgata
    10921 aaactggctg tcgcactcgg gaaaaaaact tagacaatct gctcattttc aaagacaaaa
    10981 aagaatccga agcttttgct aaaagaatcg gcggtcgtgt caaaccgata caagaggtac
    11041 gaccagattg attcaatatt tttcttttcg aggctctaat aggcatgcct atcagggctt
    11101 tttttgcaga ctgtgttttt atgcccacac gatttgtaag cgatttatgt tataatagat
    11161 tatatctgca tagaaaggaa ggttaaatgg ttatcaataa tttgcaacag caagcttatg
    11221 aatctatacg taaacaaatc atttattccg agttagcacc tggaagtaaa atctccgata
    11281 aagtgttaga agaacaacta gctatcgggc gtacaccgat tcgagaagca ctgatccaac
    11341 tgcgcaatca agagcttgta aaaaccattc cacagtccgg cacttatgtt tcaaaaatcg
    11401 atatccgatc tgcttcatta gcccgttata cgcgggaaaa gttggaaaaa ccgatcaggc
    11461 agtaatcgct caaaataaaa aagctttttt tgatttggat actgattttc accgaacttg
    11521 ttatgaaatt gctggaaaac gagaaatttg ggattggcta gaaagttata gtacgcactt
    11581 gaaccgtttc agatggttac gtttgacgat ttcagaatta gactggggtc gagtattaga
    11641 tgaacatcag acaatgcttc aatcaatgat tgatcataac tttgatgaag tcggatttct
    11701 ttgcacgatg catttacata tgattatcga ggagcaagaa tacgtgattc ataattaccc
    11761 cgattatttt gaagatattc aggacagacc catcaaataa gaggttttgg aatcagcgtt
    11821 ttgacctgag tattagagaa agaaacggca aaatagttcc tcatattttg ccgttttttt
    11881 tgagctaata ccgagggtca gctgattgca caccgtttat tgagaggttg tgaaaaagct
    11941 gccctgcatc aagtaataag aacagccaag taaaaatagc ttcttatgtt tttaacttgg
    12001 ctggacttat taccgcagat gcaagctttt gaacaccgtt ttccaagagg tcgtgagcct
    12061 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa
    12121 tattttgact tgtgaccgag gagttgacag gagaacatcg tttattcgga aaaaaaggac
    12181 tgtgacacga cttttgtcac agtctcttta tttgtaatct aaatcatact ccgtacatct
    12241 tttttctatt ttttttattt cttctaagat cccttggcta cgaatttcat tatatggcca
    12301 aagtaagact gcctctaata cataaggcga aaaatcgctt tgattcatct cttttgaaaa
    12361 actcatcgct gctatctcgc tcaatgcacg aatgtttgat tgatgtgtat aaaaaggcaa
    12421 ccgccacagc acaatttttt cgctttgcgt ataaattccc ttaacagaag cttcgatgtg
    12481 gtgaaatagt tcatgtccaa taatcaaagc ttgcgctgtt tctttagaga tcgaagctag
    12541 tcctgaacat tttaattttt ctaacggctc cgtcatcaat cgaatccctt catcttctgc
    12601 ataagttgca aaagtgattc gttcgtttat ttgctctgat tcacaggaaa cgattggaac
    12661 agccatttgt tcacaaattt ctcctacatc ggcaaaaggg tacttttttt tgatgctaca
    12721 tgccgtttcg ataccacaat cgattgattg ttgtataaga attttctgct tggacaaagg
    12781 aatctttttc caaaataaat ctcgccgaaa ctggtagaat ccccaaattt cttcggggat
    12841 ctgttgtaat tcgtttagta actcttctaa cttatgtttg tttgatattt ccggcaacga
    12901 cgatcacttc ttcctccggt tccatagtag atacttccag atccatcaaa agctgtagtt
    12961 gttccagatc cgtcgctgta aaatcagaga ctcttgctcc taagcagaga taaaattctg
    13021 gtcgggcaat caattcagtt tgataaactg ccatatcttc ccaaagttgt tcgacagctg
    13081 tcatataatt ggctgcagtt gttttcatac atgaagcatg tccacgttgg accttgataa
    13141 atcctttttg atcgatgatc cgaagacttc cagcattttt tgaacgattc tgttcgccgc
    13201 atacataaaa gaaaggtgta ttttccaata agcaaacttc atttttattt aatcgcatat
    13261 cctctgcagc aagctctagt gcttcttctg ttgtgatctc cttggttaga tcggttgctt
    13321 tcacttccgt agaaccagta gcgatagccg ttacttttga agtctgtgga tcgatttcta
    13381 cgtggacttc gatcgattct ggtgtagcgc ctgattcaat cgctttattc atcgcttcat
    13441 ttttcaacga tcgtatatct tctttacttg gcgaagggat gatacgttct acaacatctc
    13501 ggaccattgc aagtgctaca ccaatagaag aaatcacttc ggcgttttca ggaatcgaat
    13561 atttcacacc cattttatta ctaaagtaag taatcaatga ggcagcgccg ccccctacac
    13621 ctacaagaga aatctgatct ttctccaagt gatacttgtc tgccaattct aaaatcaccg
    13681 gttcaatctt agcataggat ttttccatga tctgttctgc gatgtcttca accgtcgtat
    13741 gacaataatc agctaatgct tggatggctt ttcgtgcaga tgggacattt ccataagaaa
    13801 aatgttcttc ttgcactaat ccaaggacat tggctgcaca tgtatttgta atagtcactt
    13861 cttctccgtc ttccatcact accttgacgt aatcagcagg gtctccttct ttaggagaga
    13921 aaaattcaac cttaggacct ttgatttttt ctgtctccgt aaatactgca taatctagac
    13981 cggcaatatg tgcagaacgt ggaccgacat cgatgattcc tgactgatta gcccgtacca
    14041 tcgaaccgcc tgccacacct agaacccgaa catctaatga agagatatat gttgggtggc
    14101 cacctacaat tgaataatcg attgctggac gtccattttt aatgaccccg atattggttg
    14161 ttgttccgcc tacttcaaaa taaacaccat tagacgctct caagtacatc aaagagccca
    14221 tgactgatgc tgctggaccg gaaagcatgg ttaaaacagg tcgttttttc atttcattga
    14281 tttccattac tccgccgtct ccgcgcataa tcatcaaaga aacattgacc cctgcttctc
    14341 gtacggaatc ttctgtagag gttgccgtat ctagcatttt tggtaaaata gatgcattga
    14401 ttgctgctgt ccgagttctt cttgtcaatc catataattt tgtgatgtca gaagccatcg
    14461 ttgtagggat cgattttacc gatgctgctt cataaacaac tcgttctggt tccccattat
    14521 ctactccaaa tgccatagaa gaaaccaaga cctccgctct ttctctttct aaagaagaaa
    14581 tcgtttctga aacacggtct acatttaagt gtttgacagg taagaatgca ttgacaatct
    14641 ctattttttt cttattccct aaatcgatat cgttcaatct agtttgtcgt tttgctaaaa
    14701 aaccttctaa tccacctttt gccatcccga ttacgccgac ttttgctacg tctccttcaa
    14761 tcagtgcatt agtcgcttgt gtggtagaat gtgcaacaaa cacaacatct tctggactga
    14821 tattattttc ccgcaaacaa ttttgaaaag actgaacgac acctgcggct actccacgga
    14881 catcatcgtg ggttgttttc actgaagatt ttccgatgat ttcgtgcgtc gcattatcga
    14941 ttgcgactgc tttcgtatgt gtaccgccaa catcaattcc cattcgcaca aaacgtttca
    15001 ttttcttttc ctccattcat ctaatcgttt ttatcgacgt tttgtttact aggcgttccc
    15061 gtacaataag aacgtgatca ggcataagat cgctccgatg atccaaccag ttgggatgga
    15121 tttcttcata tattctcgag aagttacttt tgtataacct aatccccaag caacccaaga
    15181 ttgtgtaata tctaaatgct gaggagcaat cgttgtgatt gcaaataacg ggaacaaaaa
    15241 ggctggcgac atcgttggat taacagctaa tacgacagct aaaatagctg agcctgaacc
    15301 gactagattc attggaccgc ggaaaaatcc tagtggtgtc aatatagcaa atacgattgc
    15361 caagactaat ggtgattgcg gtatccaatc tccaaaaata gctgaaaaat aaggagaagc
    15421 atacgcagct gaattattga acatcgctaa ggtcatcaag aaaccaacca ttggtgcaac
    15481 atctactgcg ccatctgtaa ataatttcgc aaactgacga cagatttctt tataacctcc
    15541 gcgcaatttt ccagtaaaca gcaatgccca gatacctgct agaataaaac ctaagatgat
    15601 tggaagatct aaaagaacga cccctaaaac tggtaataat acggctaacc atgagatcat
    15661 tggtgcgtcg ccaccttcag ctggtacgtt tgctgccatt gcatagcgtt tctttttgtt
    15721 cattgatata ttagcgaccg ttagtaccac aactaagctt acaatcatcc caatcatacc
    15781 aatttggaaa tagtcattat aagtatagct ttcagccgct ggattgaaac cagcataaat
    15841 cgtttgatat tgtttgaaat tgacgatatt agcaaagatt ccagccataa tcgagcccat
    15901 aaatgagaac agtgcaaccg gtactgaaat acctaatgac atcaagattg gcaacacaat
    15961 aactgcaata gaaatcactg gtccgattcc tgtcatcgac atgaatataa cagcggttac
    16021 aatacatagt aagctcatcg tgattcttgg cttgtcccct ccaagttcaa ctacctttcg
    16081 aatcagggta gctgcgattc cgctatctac tagaacacgc ccgaaaaagg caccaaagaa
    16141 gacatttacc aagatcgatt tggcgtactc agctggcccc gtcgtataga tatgcgtcaa
    16201 cacatcaatt actccttgcc cttccatcgc cgggtttggt gaaaacgtgt ttccgactag
    16261 cgctaatccc atccatacag tagccatgat ggcaaagccg accatcaagt tgtagccttt
    16321 tacacagtac cagaccatga aaaagaatga gataactaac aaaataccaa taataatatc
    16381 actcatgtta ttcctccgtc cattttgttt ttctgataag caaactaata tatcagtcaa
    16441 actcattcta acttatgtag ttagcgcttt cattaaaggg tgaccataaa tgttcagttt
    16501 tcaattaaaa aaccttaatt atgctatttt agacttatac gttattgtcc tgtttcattt
    16561 gatggaggat tgtaaataaa aaaagaggaa agaacataac ttttcgttat cttctttcct
    16621 ctttataatt gtttagctta tttagccaaa gcttcttttg cttggtcagc caatgcagta
    16681 aatgctgctg catcgttaac agccaagtca gctaacattt tacggttgat gtcgatttca
    16741 gcaactttca aaccgtgcat taattttgag tagctcaagc cattcatacg agctgctgcg
    16801 ttgatacgag caatccacaa tttacggaag tcgcgtttct tttgacgacg atcgcgatat
    16861 gcgtagttgt atgaattcat tacttgttct tttgctgttt taaataaagt gtgttttgaa
    16921 ccgtagtaac cttttgctaa ttttagcact cttttacgac gtttacgagt tactgttcca
    16981 cctttaacac gtgccatgta taattcctcc taataaaaat aaatcttcag attctgttat
    17041 ttgtacttct tatttcattt ttgctagttg ttggcgaata cgtttgaagt cgccactaga
    17101 aaccatgctt gctttacgca attgacggcg ttgttttttt gttttgccgt ggaaacggtg
    17161 acttgtaaac gcgcggaagc gtttcaatcc gcctttacca gtacgtttga aacgttttgc
    17221 tgatccgcgg tgtgtttttt gttttggcat gactatattt cctcctcaaa atctttcgtt
    17281 atccgactat cggctgtcca acagccagtt agtcattcaa attacttact gtcgtttttc
    17341 ggtgccagcg tcaagaacat gctgcgtccg tccattttcg ctttttgttc cactgttgca
    17401 atatccgcag tttcttcagc taagcgattc aagacttttt gaccaatttc tttatgggta
    17461 atggcacggc ctttgaaacg gatagaagct ttcactttat cgcccttttc caagaactta
    17521 cgtgcattac gaagttttgt attgaagtca ttcaagtcga tcgtaggact taagcgaact
    17581 tcttttacat tgatcacttt ttgtttttta cgcgcttcgc ggtctttctt ttgttgttcg
    17641 aaacggtatt taccgtagtc catgattcgc gcaactggtg gtttcgcacc aggggcaact
    17701 aacacaagat ccaagtttgc ttgttctgca atattcaatg cttctgcttt tgtcttaact
    17761 cctaattgct caccatctga accgatcaat cgtaactctc gtgcacgaat gccgtcgtta
    17821 accatcatat cctttgctat ggtcattcac ctccaaatat tatgagagaa agaaaagctt
    17881 accgaataaa cggcaataaa aaaacgagtt ctcttacaga acccgcgcgt acttcaccta
    17941 atagaaccag tgaaaactat ctagcccagt gacattatca actaaggcga gaagcgggtg
    18001 cttctgcttt tatttctcaa ccatcatagt atagcaaaaa ctaatctttt tgtcaatgtt
    18061 tttcatacag tttgtcttcc ttttctgaaa atgattagat tagcagactt tctagcagtg
    18121 attcccgctt ctttgctata ctaaagaaaa acgaggtgaa cccacatgaa aaaaatcgaa
    18181 cctattccac cttctcttcc tcttcttaca aagggagagt tttatttaga agatgaggag
    18241 atcttctctg gtcttctgat caacgggggt gaccaaagct atctcgatgc caaaaatatg
    18301 gtattaagag agagtcatat ccaaaaactt acgatgcaaa aaacagtttt acgtcatttt
    18361 gaatgcagta atgtcatttt tgaaaaatgt gatttttcca atctggagtg gattggtggc
    18421 agtttccacc aagtcgtgtt tcatcaatgt aaattaacag gtacgaattt tgccgaaagt
    18481 tatcttcgag actgtacttt cactgattgt attgcttcga tggcttcctt tagtagtaca
    18541 aacttgaaag ctgtctcctt cgatcattgt caattagaag acagcgaatt ttatgaggtc
    18601 acttggaaaa accttttttt gtctgacaat cagttgaccg gttcgaactg gtttcgaact
    18661 tctctgaagg gattagactt tacgactaat ttcttcacag caatcgcttt atctcaagat
    18721 tctttggcgg gactaatcgt caaccaagag caagcacttg tgatcgccca agcccttggt
    18781 ttgcagatca aagactagat caacgaatcc gccgccacag tgcaataagc atccaaatag
    18841 ataagaccaa actgatgatc aaggcgattc cccatccgaa aacggaatgg gtaaagggta
    18901 actggagatt cattccgaag aatcctgtca caatagttgg aacggtcaag atcagcgacc
    18961 agaccgtcaa aaatttcatc gtgtcattca agttgttatt cagcaaatta ttataagtgc
    19021 cagagagctg gtctaaaatc tgcgaagcta agttcgtcat ttcaactgct tgtcgtgctt
    19081 cgatcagcgc atcgtccaac tcttcttttt ccttttctga caacttgcga taaatagcca
    19141 atgccttgat ttgttctaaa agtactacat tttgttttgt tcctgtaact aagtaaacca
    19201 aaccgatctc taaatctgaa agttgcagca gattcttgtt cgttgttttt tctcttaatt
    19261 tcagattaag ccgctgacgt tcactattca cttcctcgat taaaggaaaa aaggaatcag
    19321 aaatcaaaaa caatgtacga aacaataaac tgtatacact catgtctggc attcgttcca
    19381 aaagcgatcg aattagtcga atcacgtact cggtattcgg cgtggtaaat gtaaacaaac
    19441 cgtcttcttt taaaataaaa gcgataggac tagtttcata atgattatcg actttttgct
    19501 ggtgcggaac atttgataca agcagtaacg cttcatcaaa agaatcgtac tctacgcgcg
    19561 cccgttcatt tttatctaga gaataagtca gcatctcgtc actaatgcca tattcttctt
    19621 ttaagttttt aacagctagc acatcgtttt gctgtacatt gatccaaaaa ttacggtctt
    19681 gattaaaata gtgtttttcc cgcatactct atcctcctga catttgtttc cagtataaag
    19741 aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc aactgtaaat caaatggcta
    19801 ctttggtttg gcgaatctct gctaattttt cttcgatcat tttcaagact tcttcttttg
    19861 cttgaggatc ttccacaaaa ttatagcggt ctccatcgat ttggattttt ggactttctt
    19921 tgtattcttc ataccattga tcgtaacgtc tgttcaattc tttgtaataa tcgtatagac
    19981 ttggatcaaa ggacaattgt tcatacgaac gaccacgttt ttcgatacgc tccaacatcg
    20041 tatcaaaaga tacacgaata tgaactaata aatccggatg ttttttatgg gcagcatagg
    20101 gaagttcttc catcatgttt tccagcagtt catcatacac acgaacttct gtttctgttg
    20161 ctcttcctag atctgcattt agatggaaaa gcagtgaatc ttcataaatc gaacggtcca
    20221 atacattatt gtcatctttc attgcttgtt tgatgcttgc aaaacgttta ttcaaaaaat
    20281 aaatttgcag caaaaaagca tactgttcag ggtttgcata aaataagggc aagacttcat
    20341 tatcatcaat cgattcataa aatgcctgac tatcaaagtg ttcagccatc atttctgtta
    20401 aacttgattt tccggcacca attgtacctg ctaaaacgat cacgctcatt tacctccagc
    20461 tcacttcatc actaaatatt gtgtttaact ccaacctttt taaatatacc cccaatatct
    20521 agtagtgtca attgtatttt tttctagtca aagaagaaaa aaagtcaaaa aattcttaag
    20581 aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt acttctgaga tggttttagt
    20641 aaaataaagg aggaatgttt tttgagaccc ttagtggaca aaaaaattcg tgaagcagcg
    20701 caactcgttt tgggcgcttt attttttgct taaaaatcac ttttacgtga gaaggagtta
    20761 gattatggca aactatggcc aaggagaatc aagcggaaag atcatattga tgggcgagca
    20821 cgcggttgtt tatggagaac cagcgattgc ctttcctttc tatgcaacaa aagtcaccgc
    20881 attccttgaa gagctggatg caatggacga tcaactggtt tcttcctact attcaggaaa
    20941 tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa ttattcattc acttaaaaaa
    21001 acagcatgac atccaaaaaa acttgcaact gaccattgaa agcacgattc ctgctgaacg
    21061 tggaatggga tcaagcgctg cagtcgccac agcagtcact cgtgcttttt atgattactt
    21121 agcatttcct ttgtctcgtg aaatactatt agaaaatgtc cagctttcgg aaaaaatcgc
    21181 ccacggtaat cctagtggaa tcgatgcagc cgctactagc agcttgcagc cgatttattt
    21241 tacaaaaggg catcctttcg actacttttc tttgaacatc gatgcttttt tgattgtcgc
    21301 tgatacagga atcaaaggac aaacaagaga agccgtcaaa gatgtcgctc acctctttga
    21361 aacccagcct catgaaactg gacaaatgat ccaaaaatta ggatacttga cgaagcaagc
    21421 aaaacaagca atcattgaaa attcaccaga aacgttggca cagacaatgg atgaatcaca
    21481 atcacttctg gaaaagctga caatcagcaa tgattttctt aatctattga tccaaacagc
    21541 aaaagatacc ggggcattgg gtgccaaatt aactggcggt ggacgcggtg gctgtatgat
    21601 tgcattagca caaacaaaaa caaaagccca agaaatcagt caagcacttg aagatgcggg
    21661 tgctgctgaa acttggatac aaggattagg agtacatacc tatgtttaaa ggcaaagcac
    21721 gcgcatatac caacattgct ctaatcaaat attggggtaa gaaaaatgaa gaactcatcc
    21781 ttccaatgaa caacagcctt tcgttaacat tggatgcatt ttatacagag acagaagtca
    21841 tcttttctga tagctatatg gtagatgaat tttatctaga tggcaccttg caagacgaaa
    21901 aagcaacaaa aaaagtcagt cagtttcttg acctttttcg taaagaagcc ggcctctcac
    21961 taaaagcttc agtaatcagt cagaatttcg ttcctaccgc agctggatta gcctcctctg
    22021 ccagcgggct agctgcttta gcaggcgctt gcaatactgc tcttaagctt ggattagatg
    22081 atctctctct ttcaagattt gctcgacgcg ggtctggttc agcttgccga agtattttcg
    22141 gtggtttcgt cgaatgggaa aaaggccatg acgacttaag ttcttacgct aagccagtcc
    22201 cttccgattc tttcgaagac gatttagcaa tggttttcgt tttgatcaac gaccagaaaa
    22261 aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga aacatccagt ttttatcaag
    22321 gctggttaga ttccgttgaa ggggatctat atcaattgaa acaagcaatc aaaacaaaag
    22381 atttccaact tctcggagaa acgatggaaa gaaacggact aaaaatgcat ggaacgactt
    22441 tagctgccca gcctccattc acttactggt ctcctgacag cttaaaagcg atggatgcgg
    22501 tgcgacaatt gagaaagcaa ggcattccat gctactttac gatggatgct ggccctaatg
    22561 tcaaagtttt agtggaaaac agccatttat ctgaggtaca agaaacattt accaaattat
    22621 ttagcaaaga acaagtaatt actgcacatg ctggtccagg aattgcgatt attgaataaa
    22681 gggagatttt tatgattgaa gtatctgcac caggcaaact ctatattgcc ggagaatatg
    22741 cagttgttga aacaggccat ccagcagtta tcgctgcagt cgatcaattc gtgacagtca
    22801 ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc acaatatagt gggatgcctg
    22861 tacgttggac aagacgcaac ggagaattag ttttagatat tcgggaaaat ccttttcatt
    22921 atatccttgc tgctattcgc ttgactgaaa agtatgcaca agaaaaaaac attcttttat
    22981 ccttttatga tctgaaagta acaagtgagt tagacagttc aaatggccgg aaatatggat
    23041 tgggttctag cggtgccgtg accgttgcaa cggtcaaagc cttgaatgtt ttttacgcgt
    23101 tgaatttatc tcagttggag attttcaaga ttgcagcact agccaattta gcagttcaag
    23161 ataatggttc ctgcggcgac atcgctgcta gctgttatgg tggctggatc gctttctcaa
    23221 ccttcgacca tccttggctc caagaacaag aaactcagca ttctatcagt gagttacttg
    23281 ccctggattg gccaggtcta tccattgagc cattgattgc tcctgaagat ttacgtttat
    23341 tgattggttg gacgggtagc cctgcctcta cttctgattt ggtcgatcaa gttcaccgtt
    23401 cgagagaaga taaaatggtg gcttatcagc ttttcttaaa aaatagtaca gaatgtgtca
    23461 acgaaatgat caaagggttt aaagaaaata atgtaacgtt gatccaacag atgattcgaa
    23521 aaaaccgaca attactgcat gatttatctg caatcactgg ggtcgtcatc gaaacgcctg
    23581 ctttgaacaa attgtgtaat ttagctgaac agtatgaagg agccgcaaaa tcttctggtg
    23641 caggtggggg cgattgcgga atcgtaattg ttgaccagaa atctggcatt cttcctttaa
    23701 tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt acatgtctat agcgatcaaa
    23761 gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca ctagctaaag cctttcatga
    23821 taaacaaaaa aacgaatttg acttcgtacg gatcatccat aaccctttgc cgcaaatagc
    23881 agtttctgat gtcgatctaa gtacacaggc agttgggttt acactaagca gcccatttta
    23941 tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa atcaaccaag atcttgcaat
    24001 cgtcgcaaga gaagcagatt tgatgattgc cactggttca gtcagcgctg ccttgaaagt
    24061 cccttcttta gccgatactt atacgattat gcgacaagaa tatcctcacg ggaaaatcat
    24121 tgccaacatc ggtgcaggga cttctgtcga aagagccaaa gaagctatcc gattgtttca
    24181 cgcagatgcc ttacagattc atttgaatgc acctcaagag ttggttatgc ctgagggaga
    24241 ccgtgatttt accaattgga aagtacttat tcaagaaact caaacagcca ttgacgtccc
    24301 cctcatcgta aaagaagtcg gttttggcat gacaagagaa acgctcaatg atctagctgc
    24361 tttaggagtt catacagttg atatcagcgg tcgaagcgga acaagcttca cccagatcga
    24421 aaacgcccgt cgctccaagc gggaactgag ttatttagct gactgggggc aatcgacagt
    24481 ctcttcattg cttgaagcaa atgaagcaga cacttctatg gaaatcctag cctccggagg
    24541 tatccgcaat gcctatgaca tatttaaagc cctttgtctc ggcgcaaatg ccgtgggtac
    24601 ttcaggaacc gtactgacgc atctgatgaa tcacggcgta gaagaaacta ttatactgat
    24661 gaagcaatgg caagaagagc ttcgtctgct ttatactatg gtcggcgcaa cgaataccgc
    24721 ggccctacac caacaatctc tgatcttttc cggaccagtg aaggattggt gcgaagcaag
    24781 agggatcgac ttagtgaagt atggaagacg cacagaaaag agcgtgggac aaaagtaaag
    24841 aacacttttg tctcacgctc taaacacgta caaacggcgg aagtaaaagc aactccttcg
    24901 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaattcc ttcttatttc
    24961 ttggagttaa atgcttctgt cccagcctct tttttcgaat aaatgatgtt taatcagctg
    25021 tccttcggta ttcgttcaat ccgctgattt cacaacctct ccactaaaca gtgtactaaa
    25081 gctcttttaa ggttgttttc cgatataggc aagtatgcct ccgtccacat acaagacatg
    25141 gccgttaacg aaatcagatg cgctagaagc caagaagaca gatggtccag ccaaatcgat
    25201 tggctcgccc cagcgtgctg ctggcgtacg tccaacgata aattgatcga atggatgacg
    25261 ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc ggtgtagcaa tataacctgg
    25321 tccaattcca ttacattgga tattgtattg accgtattca gaagcgatgt ttttcgtcag
    25381 catttttaat ccgccttttg ccgcagcata agcactgacc gtttcgcgtc ctaattcact
    25441 catcatggag cagatattga tgattttacc gccgcctttt tcgatcatat ccggaataac
    25501 tgcttttgaa acgatgaatg gggcatttaa atccacgtcg attacttggc ggaagtctgc
    25561 cgcatccatt tcgatcatcg gtgtccgttt gatgatccct gcattgttga ctagaatatc
    25621 aactgaacct acatcttctt tgatctgttg aaccatcgct tgaaccgctt cttcatcggt
    25681 cacatcgcag acataacctt ttgcatcgat gcctgcttct ttataatgcg ctataccttc
    25741 atcaactgat tcttgattca agttattaaa tacgattttt gctcctgctg tagctaaaga
    25801 gcgtgcaatc tcaaatccaa taccatagac agcaccggtg actaatgcta cttttccatc
    25861 taaacgaaac atatccatac taaattccat cattttctct cccttatgct tattttaatt
    25921 gatccatttg caccatatcc atgtctgtat aggtgatatt ttctccgcac attgcccaga
    25981 tgaatgagta attgcttgta cctacacctg aatggatcga ccagcttggc gaaataatgg
    26041 cttgttcatt gtctacaact aaatgttttg tttcatctgg tttgcccatc atgtggaaca
    26101 cacgtgtatc ttcattggca taatcaaagt agacataagc ttccatacga cgttcatgtg
    26161 tatgacaagg catcgtattc caagcgcttc ctggttctaa aatcgtataa cccatttgta
    26221 attgacagct ttcgcataca tttggatgga tgtattggta gattttacgt tcattcagtg
    26281 tcaggccttc accagtctcc attggtttta cttggtcgat actgattttt acatttggat
    26341 atttatgatg agctggaaca gaactaatat agaatttagc tgggttttca gcatcttgag
    26401 aagaaaatac aacatgtcgt gtttcttttc cgatatagta tccgtcttgt tttttcattt
    26461 cttctttttg cccatcgatc tcgatgaatc ctggtccacc gatattgatc acacctagtt
    26521 cacgacgctc taagaagtaa tcaacaccta gttctttgtc taatgtgatt tctaattctt
    26581 tttcagtagg tgtcactcca ccaaagatca ttcgatcgtt gtgcgtataa gtcaaactga
    26641 ttttccctgg ttcaaatacc ttttctacta aaaattcttt tctcaattgt tccgttgaat
    26701 aatgacggat atcttctgga ctgtgtgtat aacgtgtttc catttcttgc attttcttct
    26761 actccttcta tcgaatgatt tttccagagc cgcatgctaa aaattgtgcg acttcttctt
    26821 gactaaattg gttgcagtcc ccatggatcg tatgcttcaa ggcagaagct gccgtagcaa
    26881 aatcaatgat ttcttgcgga gacatattgg ataaaatccc atggagcacg ccacctgcaa
    26941 aagcatctcc gccacctact cgatccacga ttggggtgat tctatggact tgggattcca
    27001 catattcatg gttcatccac aatgtcccct tcaattcatt ttcacttgct gaatgaacgg
    27061 tacgcttggt cgaataaaac acctgaatat tcgggaactt ttcctgcatt ttttgataat
    27121 aataaattag aggttgttct tctgcttctg gcgctttctc gatacctaac aaataaagag
    27181 catccatttt tccagctgag cagatatcta ctaaaggtaa aagctgatgg attacttcgc
    27241 tggcttcttg ctgtgtccat aattttccac gatagttgat atcgaagctg atcaggcagc
    27301 cagctttttt tgcagcttct atcacctgtt tcgtcatttt ttgccatttt ttagacaaag
    27361 ctggggtgat tcctgaaaca tgaaatatat ctattccttg gaataactcc tctagcgacc
    27421 attccaaatg atccatttca gcaaaacttg atccggcacg atcatagatc actgaggcgg
    27481 cgcgttcgcc actaccagtt tccatataat aagtacctag tcttgatcca ccagataata
    27541 cctgtttcgt atctactcca taacattgca agtgcttttt gactgctttt cccaatgcgt
    27601 tatcaggtac ttttgtagca aaaatcgctt catgtccgta attagctaaa gaaattgcta
    27661 cgtttgcttc ccctccgcca taatgggcag cgaatcgttc acttgaatcg atacgggttc
    27721 cctgctctgt ggacaatcgc aacatgattt ctccgagtgt cactactttt cccataagca
    27781 gctacctctt gatttctttc atttttgctg catactgttg cgcgacttcc gttactttgt
    27841 caaaatctcc tgtagctgcg ggtgctaaaa ggttcccacc tactccgact gttacgacac
    27901 cagcatcgaa ccactctttc atattgtcca gactcacacc gccagttggc atgatattga
    27961 cttgaggcat cggcgccttg aaggcagaaa tgatacttgg tccataagcg cttccaggaa
    28021 atagtttgac gatatctacc ccgcttttca gcgctgtttt gatctcggta atagtcatac
    28081 atcctggtaa gtaaggaatt tgatacagat tgcacatctc tgccgtttct tgatcaaagg
    28141 aagggctaac aatatactct gcgccagcta agatagccaa gcgagctgtg acagcgtcta
    28201 atactgtgcc tgcgccaatc actatttctg gttggtcttt gtagaatgac aaaagttctt
    28261 tgattacttg atctgcttga gggactgtaa aagtcaactc aataccagtt agaccacctt
    28321 tgatgattgc gtggcaagca ttcaaagctt cttctttgct ttttccgcgt accaccgcaa
    28381 tcactccggc gtttttcaat cgagataaaa tatcaatttt tttcatcttc tccacctatt
    28441 tcctatttag aaattttatt ttataacttg gaaatctata cccatattat acggcagaaa
    28501 agaaaaattg caaacgcttt tttcattatt tttgaattct ttgctcgatt tcgctttttg
    28561 tttgcttgag ggcactgatt atttgttctt ttgtctcatc tgttaaacga tatttcggca
    28621 tacttacact aaatgcacca atgatttgtt cgtttttcac gattgaggct cctatacaaa
    28681 aaatatcacg ttcgatttct tcatcgtcaa atgcgatgtg ggtttctttt accgtttgga
    28741 tttccttttt caaccgtaat gcattggtga tcgtatattc tgtgtaaggg atcaacggat
    28801 gcttttcaag ataatcagtg tactgctctt cactgaattc tgctaatacg gctttgccca
    28861 tcgcagaatt gtataatggt cgggtgattc cgatttttga agacatgcgg atcgtttgat
    28921 gttttggatc tagtttgttc acgtacaaga tctcattgtt gtccaatacc cctaagtgga
    28981 tagtctcatc gatcttttgc tgcagctgtt ctaaaaacgg taaagtcaac tctaccaaat
    29041 cgatttgttc aatgttttta ttcgcgtagc gaataagctt tgcacctaaa cgatagtttt
    29101 tctctgaatt tttatttacg tagccgatca tcaacaaagt atctagtatt ttcaagactg
    29161 tagaagaggt cattcccaca ccctttgcga tcgtctgcaa agagacatct tggttttccg
    29221 ctaaatagtc tagtatttta gaggctttga tcaatacagt tccataaggc tttttatttt
    29281 ccatataata ttctcctatt tctttataag aaaaattata gcggaaatac catcaattta
    29341 aaagagatat ccagatataa actaccattt ctctttacag gcaatttaat gttgaagatc
    29401 cgcttcgttt ttacatcaaa ctttgatata aagcaatgat ttcttctact gtcgtttctc
    29461 ttggatttcc tggtgtgcat acatctcgca aagcatcttc tgcaattgct ggaatatctg
    29521 cttcttttac acctaattca gaaatggttt ctgggattcc aaccgctttg ctcaaccgat
    29581 cgatctcacc acaagccgct tctcttacat cttctaaaga catttcagct gctcctttaa
    29641 tccctagaac aagagcgatc tcacggtact tttctccagt atattctttg ttatatttca
    29701 tgacagttgg caaaagtgct gcacacgcta cgccatgagg aatgttgtac catgcactta
    29761 acgggtgagc catcccgtgt accagtccta aaccgacatt tgagaagccc attcccgcaa
    29821 tatactgtcc taatgccatt ttttctcgcc cattttgatc gccatctaca gaaacttcca
    29881 aagaatggcc gatgatttcg attgctttta aatgcagcat atcagtcatt tcccaagcgc
    29941 cttttgtgat aaacccttca atagcatgcg tcatagcatc catacctgtt gatgcagcca
    30001 attttttcgg cattcccatc atcatatcgc tgtcgatgaa cgcgacgatt gggatatcat
    30061 gaggatcaac acaaacaaat tttcggtgat ttacttcatc tgtgatgacg tagttgatcg
    30121 ttacttctgc tgctgttcca gaagtcgttg gtatagctaa tataggtaaa catggatttt
    30181 tcgtatcagc aacaccttct aaactaatga cgtctgaaaa ctctggattc gtcaaaataa
    30241 tcccaatggc ttttgccgta tcgattggcg aacctccacc gattgcgatc aaacagtctg
    30301 cctctgcctc tttaacaaaa gccactccat cttttacatt ttggatcgtt ggattaggta
    30361 cgatcccgtc gtataaagca taagcgattg ctgctttatc tagaagttct gttacttttg
    30421 ttgctatacc gtgctcgatc aatcctttgt cagtaatgac agctgctttg ccgaatcctc
    30481 gctgctgaaa ttcagggacg atcgactcaa tcgctccttt tccaaaatac gatgtttcat
    30541 ttaaaatcat tcgattactc attttctatt cctcctaatt tgtatttttt ttcacaataa
    30601 aaacgaaaga accccaagca accggttgcc cagttgcttg ttgctacagt tcaaagactg
    30661 gcatcaattc ccaactgact ggagaggcat ctggatttgt ctccatgata tcttccatgt
    30721 acttccacca tttttgattg atagatgtat ttgccatttt ttcccataac ttttcatctt
    30781 cgatcactaa gtaaccaaat aacaaacttg tactcggatc aagatagatc gaatagtcga
    30841 tgaccccgtg ttcacgaagt gctgtacgca tttctggcca aagttcatca tgtctttttt
    30901 tgtattcttc ttgtttttct gggtaaactt tcatgcaaaa agcttttttc accatgtcag
    30961 acacctgctt tcgtatgaac ttcctccaaa taacctgctt ttggagtgac accaaacgca
    31021 tctgctaaat gccacaggtt ttcgtccgtg attgtttgca agattggtcc ttgcgctttg
    31081 acgtacgtat agacttctgc tgccttttct gctgtctcga tcaaaccaaa gacttcatcc
    31141 atgtcttttc ctgacccgta gattccatgt tgtggccata aaaccaatcg agtttctttc
    31201 attttttcag ctgttgcttc cccaatctca tttgttcccg gaaccatcca cggaatgatg
    31261 gaaattcctt ctggaaaaac aactaagcat tccgtacaca tttgccatag cgtacgacta
    31321 aaggaacgtt cgtctaattc atgcgtaaaa ctcatcgcta atagatgact agcatggcaa
    31381 tgcataacga tccgattatc tgggtctact tctaaacggg cgatatgact catgaaatga
    31441 cttggcaact cgcttgtagg aaggcattcg ttttccagcc cccacaaaag ttccagtgtt
    31501 ttgccatctt cagcaactcg aacgattcca agattctcgg caggttcgct cgctacattt
    31561 ttaaaatatc ttcctgaacc agttacaata aaatagcatc ctgctaaacg gctggcatca
    31621 aaaatcatcg agatgcggcg gatcactttt tcaggatcca agaacggtgt gatctcttct
    31681 tcttttagta aataagaaat attcccgcca tttctttcat cccatcccaa gcgataaaga
    31741 ttcgacgtcg tttcgatcat ttctttcaca taaggcgctt gtaaaatatc tatttttttc
    31801 aatatagcca cactcctatt ttcttaattc caacacgtct ttttgatatt cctggacttc
    31861 ttccagccaa tccaaaccaa ctggaacttg atttttgtaa cagaaataat tccatacatc
    31921 ggcatacggg aaatctttca attcttccgt ataagccatt cttgaagtaa agtcaaaagc
    31981 cagctccatt tctttcaatc gctcaactgg ttccagcata gctttcagca gtgctttttg
    32041 tgtatttctc gtaccgatga cccaagcagc gatacggttg atcgttgcat caaagaaatc
    32101 cagaccgata tttgtttttt ccaacaattc gttacggact aattctttag cgatttcctg
    32161 caattcatca tccatcaaaa ccacatggtc tgaatcccaa cgcactggac gggaaacatg
    32221 caacatgatt cctttgctga ataaagctaa tgaagataat ttattggaaa tcacttctgt
    32281 tggatgaaaa tgtccagcat ctagacaaac aagtttgtca cgagtcaacc cgtaacccat
    32341 ataaaattca tgggaaccga ctgtataagc ttcagcgcct aaaccaaata gtttgctttc
    32401 tactgcatct tgtacatacc gttcatctaa ctcttctgta aaaatttcat cgagtgattc
    32461 cattaaacgt tcccgtgggg taaaacgatc aatcggatta tctttcatcc catcaggtac
    32521 ccaaaagtta ttgatacttg tttgcccagt ttctttccca aagtactccg cgattttgcg
    32581 gcttcttttc ccgtgttcga tccagtattc acgaacttct ttatctggat gagccaatgt
    32641 aaagccgtct ttcatcattg gatgcgaaaa gaaagtcgga ttgaagtcaa gaccgatttc
    32701 ttcttttttt gcccactcta cccatttttc aaaatgtttc ggctcaattt catttagatc
    32761 gacagattct tctgtatcca aatagatagc atgaaggttc aatttgtgtt tgcccggtat
    32821 caacgaataa gccttctcca gatcttgtct aagctcttca ggtgttcgtg cggctcctgg
    32881 aaaattccct gtcgccatga ttccaccggt caattcgcca tccggtgtca agaaaccttt
    32941 gacatcatct ccttgccagc aatgcataga aattttgatg tcctgtatct tttctagtac
    33001 agcatctgta tctacaccga tagttgcata tttttctttt gcttgttcgt aatttctttc
    33061 aatcgtgttc atacgattac tcctttgatt ttattcacgt accattccat cgtgttttgc
    33121 ttattttcat ctgggtaaaa agtttcacat ggaaacgact gtttgattac atttcttgct
    33181 tgttctatat tcgaaaactg acctgtactg agcatttgca tggcaacatt cccgattgct
    33241 gtggcttcac taggtcctgc ttctacaatg attccggcat aatctgctgt caattgattc
    33301 agtagacgat tattcgatcc gccccctacg atgtgcagct tttccaattt cgttccagtt
    33361 aagtgttcta ctttgatcag ctcttgtgcg taacaaagag caagattatc atacacacaa
    33421 cgagcgatag ctcccgatga gacagggact ggctgctctg tttcaaaaca gtattgctga
    33481 atctcttcta tcatatttgg gggattcaaa aatcgagggt cattgatttc catcacttgc
    33541 tgcagcggtt tttcttttgc agcaagctct gccaattccg cataactgta ctcgtactct
    33601 tgcattcggg caacttcctg gatcaaccac atccccatga tgtttttcaa aaaacgcacc
    33661 gtgttatggg caccccattc attcgtataa ttttcagaaa aagcattgcg agaaacattg
    33721 gcgatctttg tttcaatccc taaaagcgac catgtcccac tgctgatata accccaacgt
    33781 tctccttgac ctggcgttcc taatacagct gacgccgtat catgacttgc tactgtgata
    33841 aaggtcacat ctggcaaatc atattttgaa aattgttctt tttgcagatc tcctagtatc
    33901 gttcccggct ctgtcaatgg tggaaataat tccttcgaga caccgatttt ttcaagaagt
    33961 tctatttccc attcttttgt ccctgcattc agcagctgca tagtagatgc attagttttc
    34021 tccgtcacca tgttaccagt aaagacatac cctaaataat caggtatcag taacaattta
    34081 tgtgcttttg ccaaattttc tttttcttca gcaaacaatt ggaacaaggt attgaaaggc
    34141 tggatctgaa tccctgtctg ctcatacaac ttatctagag aatagatttc agaaaactgt
    34201 tctactgctc cattggtacg cttgtcccga taagcaatcg gatcatttat ccgatcccct
    34261 tgttcatcaa tcagacagta atcgactgcc catgtatcga tccccgcaaa aaggcgatca
    34321 atccctgtct gctttaattt ttcaaatcca atcaaaatct ctttgataag ataatcgatc
    34381 ttccagcgat cgtgaccgtc tttgttccga aagccgtttt tgaaccggtg tacttcttcc
    34441 aagatcaaag aaccttttgc atccctcgta cttttcatta gtcgtccgct tgatgcaccg
    34501 atatcgatgg caacaaatgt tggattcatt tctccacctc ttttacttta ttgttgatta
    34561 gcactttatt aataaagcgt tatcatttat taacaatgct atcataatac agaatcttat
    34621 attttgcttc acactatttt ggtatataat tgcattatct tgacaagttg ggagtgttta
    34681 catggattac tgggaaaaag ccaaccactc atggtcggaa gactcaaaaa gagtgatctt
    34741 gacgcctaca caaagaagca ggaatttatt tttttatatc caagaaatcg gttacttcaa
    34801 agcaccaatc ccttactata ctgaacgagc acatttacct tcttacttga taaagtttac
    34861 tttgtctggc aaaggttcgc ttacatataa tgataatcag caacagctaa aagctggaga
    34921 cttatttttt attgactgcc aagaatatca gcgttatcaa acgatcagcg aagaaccatg
    34981 ggaaatggat tggatccatt tgaatggtca gaacgtccaa tcattttatc aggagttcac
    35041 aaaagacggc cataacacct tccattcaac aggggatcca tcagaaaaca agatccacca
    35101 tttgatcaca gaattgatcc acatgcaaca aggcacgaat gcccgtaccg atttccagtc
    35161 ctctgtagct atccacgagt tgctgaatga actgattttg caaaagtatc agctagattt
    35221 cgaagaagag gatattcccg cctacgttta caaactgaag caatacttag acgaacattt
    35281 caaagaaacg attacacttg atcaattaga acaacaattc ttattgaata aatatcagct
    35341 gagcaaagat ttttccaaat ttatcggttc tccaccaatc gattatttga tcagcaaaaa
    35401 actttcttac gctaaagacc ttcttcggta tacagaattt acggttcgaa tgatttccga
    35461 agaaataggg attgagaatt ttgcttactt tagccgcctg ttcaaagcaa aaacaggact
    35521 atcgccgaga gactttcgta aattcagctg attcatataa tatgtacgtc attttttcaa
    35581 aatattttga aatatataga taaaaataaa ataaccgcag tttctgcttc tatgtaaaca
    35641 acagcagcta ctgcggttat tttttagtga tcattctcat tgttcatctt caaaatgttc
    35701 ataatgaacc aactgtttct gtgtatcacg tttcaaaaat tctttaggcg ttatgccgaa
    35761 ctctctcttg aatgctttca gaaaatgcgg ataagaagaa aaaccacttt tactatatac
    35821 agcactagag ctgcggtatt ctcgcaacaa gtgcttggca tagatcagct ttttcttcat
    35881 tacatactga tgaaacgtaa atccagtgta ctttttaaat tctcgtgtga tatggtattt
    35941 gctgacaaaa aactgctttt ccatttcctc taaagaaagc ggatgagata aattttttga
    36001 gatatattgt aacatttgtt ccacacgctc attaggaact acagcattct gttcttgcgc
    36061 actctcttct ttttgtacaa cttgattgag aaaaatcaag tattctacca gagcttgttc
    36121 atattttatg tcttcaccgt aaattttttt atcaggagga tggtcgataa atgataattt
    36181 ttgcttcaat acttcctgtt ctatttgcaa tacacgactc ctcttctctc ccattggttg
    36241 aaaacaagcc gatagattag accatggcgt ggaacgactt tctagaaaag aaggggtgac
    36301 aaagatatag acacgctcga atacatctga agattggcgg acgatacgat gcaaatcatt
    36361 ggaatggatg agcagcacac ttcctgcttt gatcgtaaat tgtcgtccgt tcaaataaaa
    36421 agtcgcttct ccctttaatg tagcatggat ctcatagaaa tcatgatgat gatagatcgt
    36481 tttattttgg ctgacgacat ctttgacatg atagatttca tactgatcag aaaccatcgt
    36541 attgacttgt tcaccgctga ttttcttttg gagcatgtgc cgcacctcct tttgttcact
    36601 tatgcacaat tttattatac cacgtttttt gtaatcaaaa agagctgctt ttatatactc
    36661 ttataaaaaa gaagagacgt ggcatcttct agccccgccc cctccattct aaatattagt
    36721 taaccaacgt cttctttaca ttattcaaga agacttcttc cataacgaat cccatcatct
    36781 tttgtttctt caagaaccac atacaggtac ggacgattct tcatgatgat ttctttgatt
    36841 tttgtgtact cgatcacacc gtctcccaaa tttaccggca cgattttttc ctgttcgaca
    36901 cgaaaatctt tcaaatgaac cgcacatatc ttttcaccaa aacgttcaaa agcttcttct
    36961 accaattgga cttgttcttg ataatcacta cttgtgatca aattggtcgg atctaaaatg
    37021 attcctagat agtccgaatc tactgcttgg atcagctgct ctacccttgc taaagaatac
    37081 accggatgat tcaaacctgg ttctatcccc accatcattt ggtatttttc tccagctttt
    37141 accaagcgac ggataacgga cacagcttca gcaaatgctt catctgtgaa attttcttcc
    37201 gtatattgga tttccggcaa aacacatcca gtttccgaag caaccatcga cgcaccaaag
    37261 gaagaagcat agcgaagata ctgctcgaac tttgtcagca actcttgtcg tatcgttaaa
    37321 tctggatgaa tcatattgat atagcaactt aaaattccta cggtcacttc ttgcttttct
    37381 aaaactcgtt tgacataatt tcccatccca gaattgatct catttgcgcc agaaggtgtt
    37441 tcaggaaacg aaagacctaa ggcgagctgc agtgtatgaa tcccttgttc ttttgttttt
    37501 tctgcaagat cttctatcgt ttggacttcg gaaagatcat gtcctctaat tgtaatgttc
    37561 aacataacta ttctcctaac tcagcatatt gggtagccac atgacgattt gcgggaagta
    37621 agtaatcaaa aacaagacga ctaagataac cccataaaaa ggaagaagcg gtttcagcat
    37681 tgcctctgct tttacgcctc ctacacttgc accgacatat agtcctgtac ctactggcgg
    37741 cgtgatggag cctacacaca gattcatgat tgaaaacaac ccgtaatgta caggatcaac
    37801 ccctacactt gtgacgattg gtaaaaatat tggtgtaaaa atcaagatag caggcgccac
    37861 atccataaac atacccacca gtaataacac aagattgact aataacagaa taataaattt
    37921 attatccgtt acgcccataa tcaacgaact aattgcctga ggaataccgg taaacgccat
    37981 ggcgaaagac atcatggaag aagtagccaa taagaacatg atcgtccctg acattgctac
    38041 tgattgaaac aacatattag gtatatcttt tagttttacc gttttatagt aaaacatagc
    38101 aatcaacagg gagtaaatga cagctatcgc tgaagcttct actgctgtaa atatccccgt
    38161 caagatacca ccgataatga tgacaatcaa tagaaggcta ggaatcgcct cttttattat
    38221 ttttccagca tgagccatct gtcctttttc gacaacaggg tatttattct ttttagcaac
    38281 gatgaatgct actagcatga ttcctagcgc ccacaaagcg cctacgagat aacctcccat
    38341 aaacaaagca gagatcgaag caccaccagc aacaagtgaa tacatgataa aagcagtact
    38401 tggaggaatg accatacctg tcggagctga cgcgatattg accgctgtcg caaacttccg
    38461 gtcatatcct tcttccactt gctgagggat caacactccg ccaatcgcgg tagaagcggc
    38521 aatggccgag ctagagatgg aaccaaataa agcattccct aacacattcg tatgagccaa
    38581 agcccccggc acgcgcccga cgaacaacat cgcaaaatcc actagtttct tagctatccc
    38641 tccattattc atgataatgc ccgagagaac gaaaaacgga atagcgatca gcgaaaaact
    38701 attgatactt cccaccattt tttgagctga actaaaagca gaaacatcaa acggtataac
    38761 taacaataaa gtcatcatcg atgagaatgc aatacttata gcaatcggca tccctaatac
    38821 aagcaacaca acaaatacaa aaaataacac taatccggct tctattgcca ttttattcct
    38881 ccatttttat attttttctt gaattgctgg cttgttgctt aatgtatcga ttagattgat
    38941 caggctatag ataatgatga gtacccccga tacaggtact gacagataaa catatcccat
    39001 aggaatactt aaagaaggcg agatctgccc cattgtcaat gtgacagcat tccatccgcc
    39061 atacaccatg atcatcgcag caaataccag aaataaactt tgaacaacaa cttctaaaat
    39121 tttacttttt ttgccagtta atttttcact taaaaccgtt acggctaaat gtgctttttg
    39181 tcccacaaca tacgcggaag acaacatggc aaaccagaca agaccgaatc gaacaagctc
    39241 ttccgtgacc gtatttggat ttcctaaaac cgttctggaa aatacttgcc ataaaacaac
    39301 agcgaccatc ccaacaagca aaacagctcc caggatctct aataccttgt ttaaaatcat
    39361 tttcatcaca gctacgctcc ttgctcttgg atcttttgat aaaggtcttt gtaggtagga
    39421 tcgtttttga atatatcatg aagtggggca actgcctctc gaaacggtgt attatctaca
    39481 tcattgaatt ggactccatg ttcttcttgt gctaaagctt tttcttcttc aattgctttt
    39541 gcaaaagctt caatttcaaa aaccgttgat tctttcgctg cttcttcaat ggctttttcc
    39601 tgctcatcag ttagctgttc tttgatggca tcattcatga tgatgatatc tggcacacgt
    39661 gtatgctcat cataggaata gtatttagcc acaccaccat gtccagctgt atacaagaca
    39721 aattcattgt tttctgatcc gttgatcaaa ttggattgaa gagacgtgta cacttcatca
    39781 cttcccatag gtacgggcga accgcctaaa agctctacca ttttgatagc ggtttcgctt
    39841 tgcatcacac ggatcttctt tccctccaaa tcttccggcg tatggatcgg accgtctacc
    39901 atataaaagc tgcgctgacc agaatcataa taggtcaatc cggtaaatcc taaatttttg
    39961 gtcgattggt agattggctc cattatttct tcattatcca tcaccttaaa gaaatgttct
    40021 tcatcattga atagataagg aactgaaaaa atcgaataat ctttagagaa actttccaac
    40081 gctgaggcac ttacttttgt gaaatctact gctcctgact gtgtcaattc gatcagttca
    40141 cgttctccac ccagttgtcc atctgggaaa tactggatct gtacggaacc atctgttttt
    40201 tcttttacta aacgcccaaa ttcattcatt gcatcaatta ctggctggct gttgcttgca
    40261 taagcaaagc gcaaggtaac tatctcattt acatctgcat ctgcgacagt tgccacacta
    40321 gaattatttc cgcagccgct tagacctagc agacctaggc taagcgaact tgctgcaaac
    40381 agcagtgatg tttttttcat atgggtttcc tttcttttaa caataagaga cgagcaattc
    40441 tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt gctgtgattc cgatattccg
    40501 ataaaaatct aaactgtccc ccattcctgt cccgattgaa acgttttgaa cttctccttt
    40561 ttcatcgatt tgttcaagca atccttggat agcacgataa gcgacttctt tatattcttg
    40621 tggtaaataa cgtttatgga ccgctttcaa aataccgtaa gcaaatcctg cagttgcaga
    40681 agattctacg taagaagaag gatcatccaa caacgtatgc cataaaccgc tttcagactg
    40741 ataagttttt aatgcacgca cttgtgcttc aagtgtttcg atcaataatt tgcgcaatgc
    40801 atcttctttc gataattcta agatttcaat gatctctgga atagcaatcg tcaaccagca
    40861 gtttccacga gcccaaaatg cttcggcata gttatgattt ccttcaaacg tccagccgtg
    40921 ataccacaat cctgttcgtt tatccatcaa gtatttgata tggatcataa actggtaacg
    40981 agcttcttct aaatattctg ggcgattcaa cagtttcccg atttttgcca aaggaagcac
    41041 tgtcatcatc aatgtatcgt cccacaactg attcttattt tccggtccat atgtggcatg
    41101 ctgtaacccg tcttcatttg tccgaggcat gtcattcatg acccattctg cccattgttc
    41161 taaataaggc aaatagcgtg aatctttcgt gtcttcatac aagtaagcca tagtcaatag
    41221 cggtgccatt gtattgacgt ttttgggcgg agcgccttct tccatacgat cctcaaacca
    41281 ttcattgacc acttgataag cgcgaggatt ttttgtcatc tggtaatttt tataaatacc
    41341 atatagaccg acaccttgtg gccaattcca cactgtccag cttttgtcat ctactttcag
    41401 tccatcaaag tccaacaaaa attcaccatt atcgtctttg atcgttgtca ggttttcaac
    41461 gagcaggtca attttttctt tcactaattc tttaggtaca aaaatctctt ctttaataat
    41521 catctttacg ccaccttttt aaacgctttc attttatatc cttaatatag cagtaatacc
    41581 gtccactata aatatcgctt tttgaacaaa aactgtctaa aattgaccta aatgttaaat
    41641 aacaaaagta actttcagtt acttggaaat taaaaaaaga acttggagaa acaacgtttt
    41701 ccaagttctt tttcatctat tttcttatct tttttctacg ctttcttttg cttatccgtt
    41761 gtaagcatca atgatgatct gtttcaattc agagatcaat ggttctttcg gattagctgt
    41821 cgtacattgg tcttcgtaag ctaattcagc catacggtct acggttgtat ctagtgtttc
    41881 ttgtgtgact ccttgagctt ttagactcat gttgatgccg acagattttc ctaattcata
    41941 aacagccgtt gctaaagctt cgactaattc ctctgttgtt tcgcctttca gacctaagaa
    42001 ttttgcgata tctgcgtaat ctgtatctgc acggaaatag tcatatttag ggaacatcgc
    42061 atgtttttgt ggatcttttg cattatagcg gataatatgc ggtaaaagaa tcgcatttgt
    42121 ccgtccatga gggatcccgt attccccgcc gattttatgt gcgactgagt gacaaatacc
    42181 taggaatgcg ttagcaaacg ccataccagc cattgtagaa gcgttgtgca ttttttcacg
    42241 tgattccata tcaggtgttt ttactgattt ttctaaatag tcaaagacta acttgattgc
    42301 ttgtaggctc aatccacgtg tataatctga agccatgact gaaacatagg attcgattgc
    42361 atgtgttaac acatccatcc cggtatctgc tgtgacagat gctggaactg acatgacgaa
    42421 ttgaggatcg acgattgcga catctggcgt caatgcataa tctgctaacg gatatttcac
    42481 atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact tcagaaccag tccctgatgt
    42541 tgtcgggata catacgaatt gtgttttttc tggtttgtcg atcttatatg tccgtttacg
    42601 gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg tctgggtgtt catagaacat
    42661 ccacattccc ttagctgcgt ccattgcaga accgcctcca agagcgatta ctgtatctgg
    42721 cttgaagtct gccatcagtt tcgttcctgc gtatacggta tttgtcgatg ggttcggttc
    42781 aacatctgag aagacttcga tcttcacatc atttttacgt cgtgccaata cttcacgaac
    42841 tgtatcgcag taaccgaatt ggaccatacc agggtcacaa acgatcatga cacgttcaac
    42901 gttttccatt ttctctaagt acaatagaga gttcttttca aagaagattt ttggcggtaa
    42961 tttgaaccat tgcatgttat ttctccgttt cgctacagtt ttgacattga ttaagtttac
    43021 agcagagacg ttacgagaaa cagagttctt accataagaa ccgcatccca aagtcaatga
    43081 cggaatcatt tcgttataga tatcaccgat gccgccttct gcagatggtg tattcactaa
    43141 aatacgacaa gctttcattc gaagaccgaa tttgacatgc aactcttcgt tttctgaatg
    43201 gataactgct gtatgtccta atcctccaag attcaacata ccttcacaca aatcaaacgc
    43261 atggtccgtg ttgttagctt tgatcatggc aagaactgga gaaagttttt ctcttgatag
    43321 cggataatct gggcctacgc cttccaattc agcaatcagc attttcgttc cttttggtac
    43381 actgattcct gccaattttg cgatttccat cgcagaatgt ccaacgattg atggattgac
    43441 cgcatatttt ccttcgttca tcactgcatc ttctaatttt tgcaattcat ctggttttac
    43501 gatataaact tggtgtgctt ggaattctgc ttttactgct gcatagattt ctttatccac
    43561 gatcacagct tgttcagaag cacaaatcat tccattatca aatgttttcg aaacgatcaa
    43621 atcgttcact gcacgtttaa tttttgctgt tttttcaata taagctggta cgttacctgg
    43681 acctacacct aaagctggtt ttcctgtaga gtaagcagac ttgaccattc cagctccacc
    43741 tgtcgctaag acgatcgcga tacctggatg gttcattagc atagaagtcg cttcgatcga
    43801 aggatgttcg atccattgga tacaattttc aggagctccg gcagcgatag cagcatcacg
    43861 taccactcgt gctgcttctg cagaagattt ttgggcactt ggatggaaag caaaaacgat
    43921 tggatttctt gttttcaacg cgatcattga tttgaaaatc gttgtggaag tcggattcgt
    43981 tgttggagtt actccgcaaa ccacaccgac tggttcagca atttcaatca accctttttg
    44041 gacatcttca ttgatcacac caactgtttt atcatgtttg atattatgcc aaatatattc
    44101 ggacgcatac atatttttga ttgctttatc ttcgtaaaca ccgcggcctg tttcctctac
    44161 agccatcttc gctaatggca tatgttgatc caaagctgcc attgccattt catgcacgat
    44221 atggtctact ttttcttgat caaagctttc catttctttc aatgctgcat ttgctttttc
    44281 tgccaaatca tcaatcattg cttgtacgtc aattgtcttc gttttttctt tttctattac
    44341 tttagccatc attatcctcc taatgggtca tggttatttg ttaaataatt cacatgtata
    44401 tcatacaata tttatttttc tatgtaaaca cttttttgtg atttttttca cagttatttt
    44461 taaatataaa attgctttat ttacaggtgt ttgtatagaa aaatatataa agttatttat
    44521 tattatttat atgacacacc cgattattca atattgagca atatatccca tgctcttatc
    44581 tctttctttt ttgaataaaa aatgaggtag gacatctttc tgccctacct cgtctttaaa
    44641 taagctttga tacttatttt tcttccttct cttctgtctc ttccagcgtt tctgttgttt
    44701 cagtttttac ttcaactgtc tcagctggtt cttctgttgt ttcaacagta gtcgttgcat
    44761 catttgttac gacagtccct ggtttgaccg ttttgattgc tgcacgatcg tactcaagga
    44821 agatcccttc gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct gaaacaactc
    44881 catgtaatcc gccgatcgtt acgactgaat cacctggttt catgctatct aacaagtttt
    44941 gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt gctaatagcg
    45001 cgataaacat cacaagcatt ggtaatgttc ccattttttt cacctcacta attctgtatc
    45061 tacaaaaaac actttagcag aaaaatgctg ataacactag ttttttactt aatttttaaa
    45121 agattttcct ttagaagctt tttgcgtttt ctttattgaa cccgtattct tcaaaaaagg
    45181 cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt ttcattagat
    45241 tcaacaagaa gtataaattg tggtaagaag tcaaacgaat cccaaatgtt tcatcacatt
    45301 tgatcaaatg gcggatatag gcacgtgtat agtttttaca tgtataacaa tcacatttct
    45361 catcaattgg acggaaatca tgggcatatt tcgcattttt cactactaga cggccttgag
    45421 aagtcataca tgtgccattt cgagcgattc gagtaggcaa gacacagtca aacatgtcta
    45481 ctccacggat aacaccatcg atcaatgaat cagcagcacc gacacccatc aagtaacgtg
    45541 gtttgttttc ggggatcaac ggtgtagtga attctaatac acgattcatt tctgatttag
    45601 gttccccgac agataacccg ccgattgagt aaccagggaa atccatcccg actagatctt
    45661 tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg gatgattcca aataatcctt
    45721 gacggtctgg attagcatga gctttcaatc ctcgttctgc ccatcgagac gtacgttcta
    45781 ctgatttttt cacgtagtcg taactttcat cgaatggtgg acattcgtca aaactcatca
    45841 tgatatcaga gcccaattta ttttggatgt tgattgcttt ttctggtgat aaaaacatac
    45901 gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc ttcgatattc cgcatatcac
    45961 ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc cagttcataa
    46021 atttatgcag tccgccagct tcttctacta agtcttcacc agggcgtagc cataaatgat
    46081 atgtattact taaaataacg ccagcaccca tttctttcaa ctcttctggg gacattgttt
    46141 ttactgtagc taatgttcct actggcatga acattggtgt ggggaaagtt ccgtgtggcg
    46201 tgatcaattc ccctaaacgc gcgccagtat gtttttcttt cttgattaaa cgatagcgaa
    46261 tggcaggttg cgtcattcat ttccctactt tctatataaa atatcttgac aactcccacc
    46321 atcataatcg tttcaagtgc tttttg
//
LOCUS       DAZUMI010000006        45941 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000006 DAZUMI010000000
VERSION     DAZUMI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.006"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..611)
                     /locus_tag="ACG81C_000260"
     CDS             complement(243..611)
                     /locus_tag="ACG81C_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5063456.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            761..1243
                     /locus_tag="ACG81C_000261"
     CDS             761..1243
                     /locus_tag="ACG81C_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5063457.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1280..2290
                     /locus_tag="ACG81C_000262"
     CDS             1280..2290
                     /locus_tag="ACG81C_000262"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5063458.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2405..3118)
                     /locus_tag="ACG81C_000263"
     CDS             complement(2405..3118)
                     /locus_tag="ACG81C_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063459.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3291..3898)
                     /locus_tag="ACG81C_000264"
                     /pseudo
     CDS             complement(3291..3898)
                     /locus_tag="ACG81C_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4382..4609
                     /locus_tag="ACG81C_000265"
     CDS             4382..4609
                     /locus_tag="ACG81C_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5063460.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4625..5791
                     /gene="hemW"
                     /locus_tag="ACG81C_000266"
     CDS             4625..5791
                     /gene="hemW"
                     /locus_tag="ACG81C_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5063461.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6025..7068
                     /gene="hrcA"
                     /locus_tag="ACG81C_000267"
     CDS             6025..7068
                     /gene="hrcA"
                     /locus_tag="ACG81C_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5063462.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7162..7725
                     /gene="grpE"
                     /locus_tag="ACG81C_000268"
     CDS             7162..7725
                     /gene="grpE"
                     /locus_tag="ACG81C_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5063463.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7776..9605
                     /gene="dnaK"
                     /locus_tag="ACG81C_000269"
     CDS             7776..9605
                     /gene="dnaK"
                     /locus_tag="ACG81C_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5063464.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9756..10922
                     /gene="dnaJ"
                     /locus_tag="ACG81C_000270"
     CDS             9756..10922
                     /gene="dnaJ"
                     /locus_tag="ACG81C_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5063465.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11004..11633)
                     /locus_tag="ACG81C_000271"
     CDS             complement(11004..11633)
                     /locus_tag="ACG81C_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063466.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11645..11818
                     /locus_tag="ACG81C_000272"
     CDS             11645..11818
                     /locus_tag="ACG81C_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063467.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11863..12600)
                     /locus_tag="ACG81C_000273"
     CDS             complement(11863..12600)
                     /locus_tag="ACG81C_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5063468.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12602..14104)
                     /locus_tag="ACG81C_000274"
     CDS             complement(12602..14104)
                     /locus_tag="ACG81C_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5063469.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14363..16357
                     /gene="uvrB"
                     /locus_tag="ACG81C_000275"
     CDS             14363..16357
                     /gene="uvrB"
                     /locus_tag="ACG81C_000275"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5063470.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16367..19183
                     /gene="uvrA"
                     /locus_tag="ACG81C_000276"
     CDS             16367..19183
                     /gene="uvrA"
                     /locus_tag="ACG81C_000276"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5063471.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19446..20711
                     /locus_tag="ACG81C_000277"
     CDS             19446..20711
                     /locus_tag="ACG81C_000277"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5063472.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20715..21446
                     /locus_tag="ACG81C_000278"
     CDS             20715..21446
                     /locus_tag="ACG81C_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5063473.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21581..22465
                     /gene="rapZ"
                     /locus_tag="ACG81C_000279"
     CDS             21581..22465
                     /gene="rapZ"
                     /locus_tag="ACG81C_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5063474.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22462..23460
                     /gene="yvcK"
                     /locus_tag="ACG81C_000280"
     CDS             22462..23460
                     /gene="yvcK"
                     /locus_tag="ACG81C_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5063475.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23494..24429
                     /gene="whiA"
                     /locus_tag="ACG81C_000281"
     CDS             23494..24429
                     /gene="whiA"
                     /locus_tag="ACG81C_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5063476.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24997..25653
                     /locus_tag="ACG81C_000282"
     CDS             24997..25653
                     /locus_tag="ACG81C_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063477.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25832..26397
                     /locus_tag="ACG81C_000283"
                     /pseudo
     CDS             25832..26397
                     /locus_tag="ACG81C_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27096..27617)
                     /locus_tag="ACG81C_000284"
     CDS             complement(27096..27617)
                     /locus_tag="ACG81C_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5063478.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27815..31495
                     /gene="nifJ"
                     /locus_tag="ACG81C_000285"
     CDS             27815..31495
                     /gene="nifJ"
                     /locus_tag="ACG81C_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS5063479.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            31684..32571
                     /gene="cdaA"
                     /locus_tag="ACG81C_000286"
     CDS             31684..32571
                     /gene="cdaA"
                     /locus_tag="ACG81C_000286"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5063480.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            32568..33737
                     /locus_tag="ACG81C_000287"
     CDS             32568..33737
                     /locus_tag="ACG81C_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS5063481.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            33761..35116
                     /gene="glmM"
                     /locus_tag="ACG81C_000288"
     CDS             33761..35116
                     /gene="glmM"
                     /locus_tag="ACG81C_000288"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5063482.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(35188..36993)
                     /gene="glmS"
                     /locus_tag="ACG81C_000289"
     CDS             complement(35188..36993)
                     /gene="glmS"
                     /locus_tag="ACG81C_000289"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5063483.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            37403..37693
                     /locus_tag="ACG81C_000290"
     CDS             37403..37693
                     /locus_tag="ACG81C_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063484.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            37706..38731
                     /gene="sppA"
                     /locus_tag="ACG81C_000291"
     CDS             37706..38731
                     /gene="sppA"
                     /locus_tag="ACG81C_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5063485.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            38734..39393
                     /locus_tag="ACG81C_000292"
     CDS             38734..39393
                     /locus_tag="ACG81C_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5063486.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            39550..40482
                     /locus_tag="ACG81C_000293"
     CDS             39550..40482
                     /locus_tag="ACG81C_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063487.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            40636..41925
                     /gene="tig"
                     /locus_tag="ACG81C_000294"
     CDS             40636..41925
                     /gene="tig"
                     /locus_tag="ACG81C_000294"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5063488.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            42172..43422
                     /gene="clpX"
                     /locus_tag="ACG81C_000295"
     CDS             42172..43422
                     /gene="clpX"
                     /locus_tag="ACG81C_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5063489.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            43505..44092
                     /gene="yihA"
                     /locus_tag="ACG81C_000296"
     CDS             43505..44092
                     /gene="yihA"
                     /locus_tag="ACG81C_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5063490.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(44166..44321)
                     /locus_tag="ACG81C_000297"
     CDS             complement(44166..44321)
                     /locus_tag="ACG81C_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5063491.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            44457..45785
                     /locus_tag="ACG81C_000298"
     CDS             44457..45785
                     /locus_tag="ACG81C_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063492.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        15144 a         7896 c         9852 g        13049 t
ORIGIN      
        1 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagta
       61 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
      121 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
      181 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
      241 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
      301 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
      361 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
      421 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
      481 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
      541 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
      601 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
      661 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
      721 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
      781 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
      841 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
      901 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
      961 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     1021 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     1081 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     1141 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     1201 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     1261 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     1321 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     1381 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     1441 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     1501 tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga
     1561 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     1621 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat
     1681 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     1741 aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca
     1801 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     1861 cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca
     1921 tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1981 gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact
     2041 tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag
     2101 tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga
     2161 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     2221 gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     2281 gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag
     2341 aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga
     2401 aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg
     2461 taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg
     2521 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     2581 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     2641 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     2701 ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac
     2761 ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     2821 gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg
     2881 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     2941 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     3001 tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     3061 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     3121 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     3181 aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt
     3241 gacaaataat catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag
     3301 agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt
     3361 atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca
     3421 agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag
     3481 cttgttgttc tataaaatct tctttattct gataattttc cgattctaac caaggaaatt
     3541 gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg
     3601 ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata
     3661 aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt
     3721 caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct
     3781 ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa
     3841 tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt
     3901 tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag acattcatta
     3961 aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag
     4021 tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca
     4081 ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat
     4141 attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc
     4201 actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa
     4261 aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta
     4321 gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc
     4381 tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc
     4441 gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc
     4501 atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct
     4561 tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat
     4621 ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc
     4681 attttgcgag catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc
     4741 agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc
     4801 gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa
     4861 acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga
     4921 gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa
     4981 ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa
     5041 aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga
     5101 aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg
     5161 atatctggat actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc
     5221 attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact
     5281 gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca
     5341 tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa
     5401 cctcatgtac tggaacaatg cccactattt tgggtttgga gctggtgcta gcggttatct
     5461 tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc
     5521 aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga
     5581 aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta
     5641 tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt
     5701 acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa
     5761 tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat
     5821 ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat
     5881 ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt
     5941 ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact tagaatatat
     6001 gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg
     6061 atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag
     6121 ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga
     6181 ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat
     6241 tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt
     6301 cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata
     6361 ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga
     6421 ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca
     6481 gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa
     6541 ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg
     6601 taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa
     6661 gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc
     6721 agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg
     6781 atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc
     6841 aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg
     6901 attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct
     6961 actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca
     7021 gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga
     7081 agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa
     7141 taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca
     7201 gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa
     7261 gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat
     7321 aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt
     7381 gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac
     7441 aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa
     7501 ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa
     7561 atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca
     7621 gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta
     7681 catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa
     7741 acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact
     7801 taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta
     7861 acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag
     7921 taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac
     7981 gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag
     8041 aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa
     8101 aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa
     8161 caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg
     8221 cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg
     8281 accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg
     8341 tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg
     8401 actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg
     8461 ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa
     8521 gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa
     8581 tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag
     8641 taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta
     8701 ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag
     8761 gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc
     8821 aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt
     8881 cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga
     8941 ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca
     9001 tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc
     9061 aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata
     9121 ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa
     9181 aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag
     9241 atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg
     9301 atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg
     9361 acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag
     9421 caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact
     9481 tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag
     9541 ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata
     9601 aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt
     9661 gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga
     9721 cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg
     9781 aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc
     9841 tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag
     9901 aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat
     9961 acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg
    10021 gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct
    10081 ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat
    10141 atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata
    10201 atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa
    10261 ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg
    10321 gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag
    10381 aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga
    10441 atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg
    10501 gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata
    10561 tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag
    10621 ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg
    10681 ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg
    10741 gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg
    10801 aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac
    10861 agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt
    10921 agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg
    10981 ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata
    11041 tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga
    11101 tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta
    11161 tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt
    11221 ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc
    11281 ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat
    11341 tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc
    11401 gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta
    11461 tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt
    11521 ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat
    11581 aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca
    11641 taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga
    11701 tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt
    11761 ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa
    11821 atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt
    11881 attcaagaaa tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc
    11941 atcttctaaa aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa
    12001 ccccatttcg tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac
    12061 gttcaagaca tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat
    12121 gatgtcaggg ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga
    12181 aagcatttca ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat
    12241 tgcttttttc tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat
    12301 gttttccaaa atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg
    12361 ttgtctcacc aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat
    12421 ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga
    12481 acctgaaggg ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt
    12541 taatactgtg ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat
    12601 cttacttcac cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca
    12661 agtacatgat cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga
    12721 tcttaccagt ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta
    12781 atgagataac gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa
    12841 tgattttttg cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc
    12901 cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa
    12961 gagtcaaggt gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca
    13021 acccaaagaa aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat
    13081 aaatactagc aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca
    13141 caccgattac tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc
    13201 ctttcaacaa tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag
    13261 tggaagactc actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt
    13321 attcgcctgt tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat
    13381 tttgtccttt tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt
    13441 ccaattcttg tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat
    13501 cgattgcgcc aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag
    13561 aatagccata ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg
    13621 ctccgactgt ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga
    13681 cagctaattg gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt
    13741 catctgttac agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca
    13801 aagcagcaga gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt
    13861 tttccatcaa ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg
    13921 gagcaaaagc agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca
    13981 ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa
    14041 acgcaagtgt cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt
    14101 tcataatcaa cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca
    14161 gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata
    14221 aaacataaca tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt
    14281 ttttagtaag gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa
    14341 tgaatgaaaa ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt
    14401 ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt
    14461 agtcggcggg aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac
    14521 gatttccaat cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac
    14581 attggctggt cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata
    14641 ttttgtttct tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta
    14701 tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag
    14761 ttcattatta gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt
    14821 aggctcgcct tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga
    14881 tcgcaatcag ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt
    14941 tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga
    15001 tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa
    15061 tgcactgaca ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca
    15121 ctttgtgacg aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga
    15181 acagcgttta actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca
    15241 gcgaaccaat tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa
    15301 ttattctcgg catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt
    15361 ctttcctgaa gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag
    15421 aggaatgtat aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt
    15481 gccatctgct ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca
    15541 gatcatttat gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat
    15601 ccaacaaatc atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat
    15661 gggacaaatc gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt
    15721 gtttgtaacg acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt
    15781 agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat
    15841 tcgagatctt cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg
    15901 attagacgta cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt
    15961 acgtagtgaa cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa
    16021 agtaatcatg tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc
    16081 aagacgtcgt acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat
    16141 cattaaagaa atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga
    16201 agcggtacaa gtctcgtatg aagaaatgac gaaagaagaa aaagatacgt tattgatgaa
    16261 attagaaaaa gaaatgaaaa atgcggcgaa agcattggat tttgaaacag ccgctaatgt
    16321 gcgagatatg attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa
    16381 aattacgatt cacggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg
    16441 agataaattc gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga
    16501 tacgctgtac gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca
    16561 gtttttagga caaatggata agccggacgt agatagcatc gacggattga gtccggcgat
    16621 ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga
    16681 aatcaatgac tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga
    16741 tcatatcgag attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc
    16801 tgagcgcaca aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa
    16861 aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat
    16921 ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt
    16981 catcgaccgg atcattgtga aagaagggat tcgttctcgt ctatttgatt ctttagaggc
    17041 ggctctgcga ttggcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt
    17101 gtttagcgaa cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag
    17161 actgttctca ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa
    17221 attagaagtc gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc
    17281 tatcgttcca tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg
    17341 tgaaagtttc ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga
    17401 tatcatcttg aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg
    17461 cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca
    17521 tgatacgaac agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg
    17581 tcagacatgt aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac
    17641 gaatatcggg caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt
    17701 aacgttatct gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag
    17761 actttcattc ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac
    17821 attatctggg ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc
    17881 tggagttttg tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg
    17941 actgattggc tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca
    18001 tgatgaagat acgatgtggg cagcagatta tctgattgac gtcggacctg gggccggaga
    18061 acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc
    18121 tttgacaggg aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa
    18181 aggtaatggg aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag
    18241 tgttgaattt ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag
    18301 tacattggtc aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc
    18361 taagccaggt aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat
    18421 cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt
    18481 tgatgatatc cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa
    18541 agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat
    18601 tattaagatc gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg
    18661 caaacgatat aactctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat
    18721 tttggaaatg acagtagaag atgctgtaga attcttcaag cacattccaa agattcatcg
    18781 caaactgcaa acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac
    18841 gacattgtcc ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc
    18901 taatggaaag aatttctata tactagatga accaacgaca ggacttcata gcgatgacat
    18961 cgcccgcttg ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat
    19021 tgaacacaat ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg
    19081 tggagaaggt ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa
    19141 agaaagttat acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga
    19201 gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta
    19261 gaatcattac atggtagttc tttctacact gattggtata gacaaaaact tcctataagt
    19321 tcaatccgtt caaaaaagaa ttgagcaatc ggaggagttt cctttgattg cttttttctt
    19381 tatggtatga tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag
    19441 agataatgat gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca
    19501 tgaacgttta tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat
    19561 atgcttcgga tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc
    19621 caggatttga taaagaccct gtgtttatcg agacgatgct tcgtttggca aaagaaagat
    19681 attctgataa gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga
    19741 tctcccaaca taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt
    19801 tatttgaaag actgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc
    19861 cgtatcctaa aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg
    19921 aattgccttt tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg
    19981 tccaatttga aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa
    20041 ttttaggccg gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc
    20101 cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc
    20161 ggatgatgtg tcttggtcat ccactgttag aagatcctac accagcatcg attggtaatt
    20221 acgttgtgat catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg
    20281 aaaaaatcga atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag
    20341 aatataaatt atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga
    20401 acggcttgaa tctggctcgt ttcgtcacag aagatcgagt gtttaacaaa ccctttgttg
    20461 aaacgaccta tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa
    20521 aaatcttttt agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca
    20581 aagaaaatcg ttacggtact actgtgtttt atgaaaaaga ccggtcaatc aagcgctggt
    20641 tgttgatgaa atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga
    20701 aagaaggcta gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga
    20761 cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt
    20821 tgaattatgt cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc
    20881 gatcagaaga aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc
    20941 agccgaactt cattgtgttg acttgtaaca ccgcacacta cttttttgaa gaattacaag
    21001 cggcaacgga tattcctatc cttcatatgc caagagaagc agcaaatgaa cttgtccgtc
    21061 agcatacgac agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct
    21121 atgaacgcga agtcaaaaat cttggattcg aaacagtgat tcctgatact gttcttcaag
    21181 aaaaaatcaa ttatctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc
    21241 tatattacga aatcttagaa gaagcggtag agcgcctaaa ctgtgaaaaa gttatcttag
    21301 gttgcacaga attgtcattg atgcatgaat ttgctgaaga taaccattat ccagtgatcg
    21361 atgcccaatc tattttagca gatcggacga ttgaacgagc gctagctgaa agaagcaagg
    21421 cattggatac agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaagat
    21481 atttggtcca aatctttttg taaaagaaaa agaaaactgg taatgatacg cccggaatga
    21541 tataatttta ctatgcacaa tgtgagagga gttcgtaatt atgacagata atttgcaatt
    21601 agtcatcata acaggtatga gcggtgcagg gaaaacagta gctatccaaa gttttgaaga
    21661 tatgggctat ttttgtatcg ataatatgcc gcctcgattg attccaaaat tctgggaatt
    21721 aatcaaagaa tcaggcaaag tcacaaaaat ggctttagga gtggatttac gttctcgttc
    21781 cttctttgaa gaaatccaaa acatgctgat cgaaatcgaa aatacgaact tcatcgacac
    21841 ttctatctta tttttagatg cttcagacga agaactagtg gctcgctaca aagaaacaag
    21901 acgagcacat ccaatggcga tggacggttt gatcactgaa gggatacgta aagaacgggc
    21961 gatactagac gacttgaaag cccaagcttc cgtgatcatt gatacgacga cattgacacc
    22021 acgacaatta cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc aaggatttcg
    22081 gatcgaattt gtatcatttg gttttaaata cgggttgccg atcgattcgg atattgtgat
    22141 ggatgtgcgt tttttgccca accctcatta tatcccggaa ctaagaccat tgaccggaaa
    22201 agatcagcca gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat tctaccataa
    22261 atttttggat cttctggaaa ctgtattgcc tggatacatt aaagaaggaa aaagcagctt
    22321 gacagttgca atcggctgta caggcggtca acaccgttct gtggcattga ctgaacgttt
    22381 ggcaaatgct ttagggaaaa actataaagt caatgtgaca catcgggaca aggataaaag
    22441 gaaagagacg gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa accaaagatc
    22501 gtagtggtcg gcggtggcac tggtctgcca gttatcctga aaagtttgcg aaatcaagga
    22561 gcagatatca cagcagtagt gactgttgca gatgatgggg gaagcagcgg tgcaatcaga
    22621 gaatcgatcg ctatggctcc tcctggtgac ttgagaaatg tcttagtggc gttatcggat
    22681 atgccacaat tctatgaaga tattttccaa taccgtttca aaaaggaaga tcagttttta
    22741 gccaatcata cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg gggaagcact
    22801 tatgaggcga ttcaattatt agcaaaaatg atgcatgtag atggacatgt ttatccttct
    22861 tcagaaactc ctttgacctt gcatgctgtc tttaaagacg gaacgactgc agtaggagaa
    22921 tctaaaatcg cgatcgaccg taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat
    22981 aaaagtgaag ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc
    23041 gttttgggac ccggaagttt attcaccagt atccttccaa atttagtgat accagaaatc
    23101 ggcgaagcaa tgctcaagac aactgctgaa accgtctata tctgtaatat catgacccaa
    23161 aaaggggaaa cggaacattt cacagatgcc gatcatatac gtgttttaca caagcatttg
    23221 aatcagcctt ttatcgatac agtattggtc aatacggaaa aagtgcctga agattatatg
    23281 gacccagaga tctatgatga gtatctagta caagtcgcac acgacttcaa cggattgaga
    23341 aatgaaggat gtcgagtgat ttccaccgac tttttggaat tgaaagatgg tggtgtcttc
    23401 cacgatggag acaaagtagt agaagaattg ttccgtttag ttttcggttc aaaatattaa
    23461 gcaatttatt gttggagaaa ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa
    23521 gaactaactg gtttggaagt tcatcgagaa catgcgaaag cggaattagc tgctttgctg
    23581 cgaatgaacg gctctttagg tattgtgaac cagcagttta ttttgaacgt ccaaacagaa
    23641 aatgctgcaa ttgccagacg tatctattct cttttgaaag atcattatca ggtacgaagt
    23701 gagctactcg ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat cgtccggttg
    23761 aagcaaggca caaaagatgt gttgatggat cttgatatca tggatggtat gatgttcaat
    23821 ggacacgttt cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt
    23881 gcttttatgg cctcaggttc agtcaataat ccggaaacga gccgttatca tttggaaata
    23941 ttttctattt atgaacagca taaccaagac atttgcgata tgttaaatta ttacggtcta
    24001 catgctcgaa cgcttgaacg gcgcaatggc tatatttctt atttaaaagg agcagaaaaa
    24061 attgctgatt tcttaacatt gattggtgca accaactcaa tgctccgatt tgaagatgtt
    24121 cgaatcgtac gggatatgag aaattcggtc aatcgtctag tcaattgtga gacagcaaat
    24181 ctgaacaaga cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca
    24241 aaggtcggtc tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa
    24301 catccagagg tcagcttgaa agaattaggg gaaatgattc cctcaggtgc gatatcaaaa
    24361 tcgggaatta atcaccgaat tcgcaaaata aatgaatttg cggaaaaact gcgtgaagaa
    24421 gcagtttaat ccaaagaaaa aactgataca tgaagcagga gatgcgaatg tatcagtttt
    24481 ttttaaaagt tatacttgct tttcttccgt caaactataa ttccatcttt actggagtaa
    24541 cataatttgg aaaggcttct attgttttta atacaatcgt ataaaaagcg gggagacaac
    24601 tgaatgaaga ttttagtttt gtacataaat ttttgcttaa tatagaagtc ctgatataga
    24661 taagatcaag tatttatcta taactggcct gattaattaa aaaactggaa ggaactgctc
    24721 gatcatcctt gagaactgct cttcttactt ctggtcaaga ttctcgtggt tggtggataa
    24781 ggatggttga taagttcatt ggattagcta gtcgaaaaca accaggtgta attaatgtag
    24841 aagttttaac aaggggatgg agtgatttat tcagtgataa tcataagttc ataaatagta
    24901 attgagatgt atggttggaa tacgttatac ctgaacttga actaccaaga tttggtttag
    24961 aatttgagaa tcgatgttta taaagggagg gcaaaaatgc gtttttttac aacttttact
    25021 atcattatga ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt
    25081 ttattatatc gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt
    25141 tttctattca cagttattgt atttatttgg agaactccag caccaccatt gccagagatt
    25201 acatatggag aatttccttt tagacttgag tatgaattga atgaagaatt acatgtgata
    25261 gaagacacgt taatagttga atttgatgga tttggaatga acgaaggcat agggagatat
    25321 cgtagatgga caagtcgttt agccagcgga gaagatttag tgttgctgtt agaagttagt
    25381 gataataagc aaatatttta ttttccagga cctgctaatt attacatggg agataggttg
    25441 aatggttata atcatacttt tcctagtgct tctttcattg aaagggaaag aggcattact
    25501 agaagggata ttttacatga caaagaatta ttagaacaat ttggtccgct tgatcaaaat
    25561 acaataaatg aagaagaatt gttaaatcaa tataatataa ggctagtgaa ttgggaaata
    25621 tctgaaccga ttgttaacaa ttttggtgat tagaaaacaa ttacgataaa tagaaacaag
    25681 ccaaatcatc agcaagtaaa agctgatgat ttggcctttc ttatacgagt agtaggaatt
    25741 catagcgaat attattacga tcagtagata ttttgatatt aaaggagtat tacgaactag
    25801 tatgtgagaa tgtagaggtg atttgcgaat aatgagaaat ttatcaaata gaaacaaaat
    25861 attaatcatt attgttgtta tagctgtgtt tcatctggga acgaatgcgg ttttaagtag
    25921 aataatattg ggtcctaagc caccaagacc tgcgattacc cgaggggagt ttgattttag
    25981 attagaatat gaagtagatg gggaaaggat tgtgatagaa gatacaattg ttgcattatt
    26041 tgatggattt tctgcagatg caggtagcat ggcatggtat cgcacgtggc gtttgcattt
    26101 ggcgagtgac aagagggcac aacaattatt gttacatgag ttagatgatg gtcgaaaaat
    26161 atattatcat tttggaggag ctggatttta tatggggaag ttagtagcag tgtaactgta
    26221 acaaggaaag atttgccagg tcttggcatt cgaaactcca tattttatat cgagcgtggg
    26281 agagatacat ctattgggaa caggaaaggg ttaactcttg aagagttata taatgacttt
    26341 ggaatccgtc ttgttagttg ggaacacaat ccaccaatag aaaatcgctt tgaataatcg
    26401 aaatctatta atccatttca tcagccagtg aatgttgtaa tggaacaatt tggtttagtg
    26461 ttcaaaacca ttgaaatact aggctgtgat cagcaaaaat gtatctgaat aaaaaagaga
    26521 gcaactatta aatgagagga gttaggagtt gttagcttta tacgaatcaa agtggcaaat
    26581 acagatgcgt atgcatttgt gtttgccatc ttgcgtttgg tagacgagat aggtataagc
    26641 taaagaaaca acttgtttaa aataaacgac ctagctcaac aaacgaccat ctaatcaact
    26701 aacagcatac ataaaagcaa ctatttttga agtagaattg aaatcgggat aacttaaaaa
    26761 gtgattggga tactagtgtg aatcaccgta cataaatatt actcgttaag ggaatttgag
    26821 aaattttgta tgaaaattta agaattagtt agtatgcgca agtcaaaaat atgaataaat
    26881 tatttggtgg agataatttg ctcagcttgt cataagaaat gagataatct ggcagatgca
    26941 agcttttgga caccatttat tcggaataag gaggctggga cagaagcgtt taactccaag
    27001 aaataagaag gagttgcttc tgcttccgcc gtttataggt gtttagagcg tgagacaaaa
    27061 gtgttcttta cttttgtcct acgctcctta tttttttatt ttgtttggat caattctttt
    27121 aattcctcta ttgaaataga ttcatcaaag atttctttac ccaagatgac agtcggaaca
    27181 aatcgaatat gtgcttgctc agcttcgttg atgacagctt ggctcagctg ttcgtctttt
    27241 tgttctgtca gctttagttt atctactgca aaatccgcta cttcttggag agaaagatgt
    27301 ttccactcgt cttgagtatc gaatatttgt ttgatctctt tgatcgcttt ttgaccatcg
    27361 ctgattgtga gataccggtg catcacgttt cctcgctgca agctttcttt ttctttatcg
    27421 aacaatttga tcactcgctg caattgcccg ctttggactg cttcttctaa tagctcataa
    27481 gattcttcaa accattgtcg gcaatacgga caagataggt tgatgaattc gaccattttt
    27541 ttaggagcat tcgatgaacc ataaaggatt cctttttgtg tatttgtttt cgttgcatca
    27601 atcactgata tatccatgtt aaaacacctc tttcgcttat agtttataca tgtctgacaa
    27661 aaaaaggcaa cgatttagac tgtttgtcta aaaattagat gaccaattca aatagacata
    27721 tgtgaaatat ttttataaat aaaagaaaaa atggaatgtt ttacgttata atggaacaga
    27781 taataagaga gaatatgaac aaggatgatg aatcatgcga aaaatgaaaa caatggatgg
    27841 caatacggca gccgcatata tttcttatgc atttaccgag attgctgcaa tatatccaat
    27901 aacacccagt tcgaccatgg ctgaattagt agatcaatgg tcagcagcag gaaaagaaaa
    27961 tgtattcggt caaccagtaa aagtagtaga aatgcaatcc gaagcaggag cagctggtgt
    28021 ggtccatgga tcattgaaaa ctggtgcact gacaactaca tataccgctt ctcaagggct
    28081 gcttttgatg ataccgaata tgtacaagat tgctggggaa ctactgccaa gtgttttcca
    28141 tgttgctagt cgggcagtga cgacaaatgc gttgaatatt ttcggagatc acggagacgt
    28201 gatggctgca agacaaacag gatttgctat gttagctgaa agcagtgtac aagaagtgat
    28261 ggatttagca cctgttgccc atcttgcttc tattgaagga gaagtacctt ttttgaattt
    28321 ctttgatgga ttccgtacga gtcacgagat tcaaaaaatc gaagttttgg aatatgaaga
    28381 attagctcct ctgatcaatc aggagaaatt agcttcattt cgtaaacgag ggatgaatcc
    28441 caatcatccg actgttagtg gtacgaacca gaatccagac atccattttc aacaacggga
    28501 aacgatcaac ccctattatg ataaattacc tgaaatcgtt caaaaatata tgaaagagat
    28561 caaccgatta cgaggaactt cttatgactt ggtgacttat tacggagcag acgatgcgga
    28621 agaagtcatt gtttctatgg gatcagttgc acagacgatt gaacaaacga ttgattactt
    28681 gaatgcaaac ggacggaaaa caggtttttt gaatatccat ctttatcgtc cttttccagt
    28741 ggaaacattc gtgcaaaagt tgcctcatac agtaaaaaca attgctgttc ttgatcgaag
    28801 taaagaacca ggggcaggtg gagaacctct cttattagac gttcaaagtg cgttatatga
    28861 ttctgaactt cgcccggctg ttattggtgg tcgctacgga ttggggtcta aagatgtgac
    28921 tcccgaccaa atctcagcag tttttgatga attgaaaaaa gacccaagca tacgcaaaaa
    28981 acggtttacc atagggatcg tggatgatgt gacttatcaa tctttggaac caaaggaaag
    29041 tctggatttg acagaaccgc aaacattcca ggccaagttt tggggatttg gatctgacgg
    29101 aacagttgga gcaaataaat cagctatcaa gattattgga gatcacaccg ataaatatgc
    29161 gcaaggctat ttttattatg attcgaaaaa atctggcggt ctgactgtct ctcatcttcg
    29221 ttttggagat acgccgatac gttcagcgta tcttgtcgag catgcggacc ttgttgcctg
    29281 tcacacacca gcttacctgc atagctatga cttggtcaaa ggattgaagc caggtggcat
    29341 ttttttattg aatactcttt ggagcgacga acaattagaa acgcatctgc ccttaaaatt
    29401 aaaacgttac ttggctgaaa ataacattag attctatacg atcaatgcca tgagattggc
    29461 ccaagaagtc ggtttgggac gccggatcaa tacggcaatg gaaacggctt tttttaaatt
    29521 agctgacatc attccttttg atgaggtgct tccgctgcta aaagaagaag ccctgaaaag
    29581 ctatggacat aaatcaatgg ctatcgtaga aaaaaacata caagcaatcg atcggacgac
    29641 cgaactgctt catcaagtcg cgatcccaga ccattggaga acgttggaga tgcctgaaaa
    29701 aatcaataaa aggaacactg gttatataca agaaatactt gaaccagtga atgcacagga
    29761 aggaaatcaa ctctctgttg gtacacttgt agcaaatgga atgacaactg gcgaaatgcc
    29821 tttaggaacg gcttctatgg aaaagcgggg cgtcgcttta gaagtacctg agtggattag
    29881 tgatcgctgt acgatgtgta atgaatgtgc ctttgtttgt cctcatgctg ccatccgtcc
    29941 atttttagca gatgaagagg aaatggaaga agcgccagaa ggatttatcg tgagagaaat
    30001 gcgaggcgca gacggcgtaa aataccgtat ccaagtttca gtggaagact gtacaggttg
    30061 tggtctgtgt gtagaggctt gtcccgcaaa agaaaaagct ttagtgatga agccttatga
    30121 agaacagaaa gaacaagctg tgaattgggc ttttgcaatg acattaagac aaaaagcaaa
    30181 tccagtcaaa ggaaagaata ctgtcttgtc tacacaattc aatcaaccgc ttcttgaatt
    30241 ctcgggcgct tgctcaggct gtggcgaaac accttatgta aaattattga cacaaatgtt
    30301 cggtgatcga atgatgatcg cgaatgcgac aggctgttct tctatttggg gcggtgcttc
    30361 tcctgcttct ccttatacga ccaatgaatg tggtcaaggg cctgcatggt caaattcttt
    30421 attggaagat aatgctgaat ttggttatgg tatgcttcta gcggcacaga ctcgtcgcga
    30481 ggctttggca agttcaatca aagaagtatt gccaaaagta tcagaagaac tacgagaatt
    30541 gatggaagac tggttggtac acatgtttga gagcgaagga acgcagcaaa gagcagcaaa
    30601 actgcaagct gcgatcgaaa gcgaacgatc aattgtccct gaactggagg atatatacaa
    30661 gagaaaggat ctattcgtaa aaaacagcca atggatgatt ggtggagacg gatgggctta
    30721 cgacattggt tttggcggga tcgatcatgt acttgccagc ggagcagacg tgaatattct
    30781 tgttttggat aacgaagtct attccaatac tggcggtcaa acctcaaaag caacaccagc
    30841 ttcggcaatc gctaaatttt cggctagtgg aaagtatgct tcaaagaaag atctaggaat
    30901 gatggcgatg acatatggaa atgtttatgt ggcgcaaatt gcttcaggtg ccaatcaaat
    30961 gcagacgata aaggcttttg aagaagcaga aaagtatcct ggaccttctt taatcattgc
    31021 ctatacgcct tgtatcacac atggtttgct tggtggaatg aaagaatcgc ttaaagaagc
    31081 aaaagaagct gtttcttctg gctattggtc attatatcga tataaccctg caatggtcga
    31141 aaaaaacaag aacccaatga ctttggacta caaaaaacct gatttctctg ctatgcctga
    31201 tttcatgagg aaacaagttc gcttttcctc gctagagaac gcaaatccaa atcttgcaaa
    31261 agctttattt gaaaagacta tccaagacgc taaaacacgt ttttacaact atgctgcact
    31321 gacgggagaa ttagaaaaaa tcaagaaacg acttgaacca gaggaatcta tcgatgtacc
    31381 cgttaagact agaaaagaaa aaacgacgga ccttgaagca gaagaaagaa gagctgccag
    31441 agcagaacga cgtaaaaaaa tggaacaagc tacaaaagaa aaaaaggaaa aataacagaa
    31501 aaaatataat gaataattct ctgaagttta aaaatattta ttgcagagaa aaataaaacg
    31561 catacttgca attttcctga aaattatgcg tttttctcaa gaaaaatacg agcagatcag
    31621 cctctaaaat taaaacatat gatatactag agaggaaatc tcaaaagaga agagaggctt
    31681 tttatgtcat ttcaaatcgg ggaacttttt aatttggact attggcagca aatgttttcc
    31741 ggtgacttgt tctctctgaa ttttttggtg aacatacttg atattctggt tgtctggtat
    31801 cttgtataca aactgatcca actgctgcga ggcacaaaag cgattcagct tctgaaaggt
    31861 gtagctattt ttattgttat ccgaatcgta gcggaaatca ttggtctgca caccttgtct
    31921 tggctgatga accaagtcat tacgtacggg gtgatagctg ctatcgtgat ttttcagccg
    31981 gaaatccgga gaggactgga acatcttgga cgcagttcgc ttttcaaaca atcaaaaagc
    32041 gaacagcagg aagatgagaa gatgatcttg tcttttgaca aagccatcca atatatgtcc
    32101 aagcgaaaaa tcggtgcgct aattactatc gaacggcata ctggattaga tgagtatatt
    32161 gagacaggaa ttgcattaga tgcagatatc actggagagc tgctgatcaa tatctttatt
    32221 cctaatacac cgctgcatga cggtgcagtt attgtaaaag aaggaaagat cgcagtagca
    32281 agcgcctatc ttcctctatc agaaagtatg ttgattccaa aagaattcgg gactcgacac
    32341 cgggcagctg tcggtataag cgaagtcagc gacgcgatta ccatcgttgt ctctgaagaa
    32401 actggggatg tcagcattac attagataat gaactgatgg ctggcttatc ccagcaagaa
    32461 tatttggcaa ttttgcgtag agaactgatt gctgaagaga aaacaaaaaa caagaaaaac
    32521 gtgattcaat ctttcctaga cggtatgact aagggagggg atcgcaaatg atctcaaaag
    32581 agaaacggac aagcattctt tacagtttta ttgcattgct gttcagtatt gcgctgtact
    32641 tcaatgcaaa tggccagtcc gttccaaata cgctttctgg aaatgaagcg tataatcaaa
    32701 cagttactgg ggtgccaatc aagatttctt atgattcaaa gcgctactat atccatggat
    32761 atgaaaatgc agtgactgtc aaactttcca gtgcaaaccg agttcagttg aatgcagagg
    32821 caaacgaaga tacacggatg ttccgcgtca cagcagatat cactaagctg ggcgaaggaa
    32881 cacatgaggt tccgctaaaa attcaaaatt tgagcagtgc tgtcacagca aagatcgaac
    32941 catcgactat tacagtaacg gttgagaaaa aggtaataaa agatttcgat gtggaaacgt
    33001 tgattccttc aacgttcaca ccatctggat atgaacttga tgatacttcc gttagtccta
    33061 aaaaagtctc tatcactaca ggtgacaaaa cattggcaga aatcaaacga gtagtagctg
    33121 atgtggacgc ttcgatggtc acagatgatg ggattaatag tgaggtccca atacaggcgc
    33181 tgaatgcagc aggggaagta ttaagtatcg tctctgatcc agtgcaagta acagtgagag
    33241 ctgattcgat caagccaagc aagtcggtac gactttatgg gatacagcaa ggaacaccag
    33301 ctgccggggt agaaagctat gatttcagtt tttcaaaact ggaagcagaa gtaagcggct
    33361 ctagcgattt gcttgcatcg atcggtgaca gtattgctgt accgatcaat gtctcaggta
    33421 tcaatcatcg aacaactcgt aaaattgaaa taccagtaga agaggggtta agtatcaagc
    33481 ccgaatcagt tagtgtggag attacacccg tgttgcaaac gtcaagcagt agctctgctt
    33541 cgacgagcag tcggacacaa gaaaatacaa gtacgagtag aagtatttca tctgctgatt
    33601 caggaacgga accatcgaca acaaccattt cttccagttc ggaatctggg acaagcagct
    33661 cgaccacaag tacagaagaa acatcagaaa caagcgaatc aacacaaatg agcagaacaa
    33721 attcctcaga aaattaagtt aaaggagtaa ttttacaact atgggtaaat attttggaac
    33781 agacggcgta cgcggtgaag ccaacaagga acttacgcca gaacttgcat ttaagctggg
    33841 acggtgcgga ggttatgtcc taagccagca tgaagaagaa ggaaaacgcc cacgcgtgtt
    33901 agtagggcgg gatacaagaa tctcaggtca aatgttagaa gaagcgctga tttcaggact
    33961 tttatcggtg ggtatcgaag tgttccagct aggtgtgatc tctacaccag gtgttgctta
    34021 tctgacacgt ttgcaaaaag caagcgcagg ggtcatgatt tctgcttctc ataatccagc
    34081 acaagataat ggaatcaaat ttttcggtgc agatggtttt aaactagtag atgaccaaga
    34141 gctagaaatt gaagcattat tagatgctga agtggatgaa ttgcctcgtc catcagctga
    34201 aggattggga actgtagaag aatttccaga aggcttattg aaatattctc aattccttgt
    34261 acaaacgatc aacggtgacc tttccggttt gaccatttgt gtcgatgcag caaacggagc
    34321 aacttcaaca agtgttaatc gtttgtttgc cgatcttgaa acagattttt ataccatggg
    34381 cacaaatcca gacgggttaa atatcaatga tggcgtaggc tctacccatc cagaacgttt
    34441 agctgagatg gtcgttgaaa aaggagcaga tgctggacta gctttcgatg gcgatggcga
    34501 tcgtattatt gcggtggatg aacttggaaa cattgtcgac ggtgataaaa tcatgtttat
    34561 ctgtgctaaa tacttggcag aaaaaaatcg attgaaaaaa gatacgatcg ttacgactgt
    34621 catgagtaac ttaggattcc acaaagcggt ggaaggaatc ggtttgaaag atgtcgttac
    34681 acaagttggt gatcgttacg ttgtagaaga aatgcgcaaa aacgattata actttggcgg
    34741 cgagcagtct gggcacatga ttttcttaga ttttaacaca actggagatg ggatgctttc
    34801 aggaattcag ttactgaata tcatgaaaca gacaggtaag aaattatcag agcttgcagc
    34861 agaggtaaca atctatccgc aaaaattagt gaatatccga gtaagtgata aaaacggtgc
    34921 gatggatgtt ccggcaatca aaaaagtgat tgaagaggca gaagctgaaa tgggatcaga
    34981 aggaagaatc ctagttcgtc cttccggtac agaaccatta cttcgagtga tggcagaagc
    35041 gcctactgat gagaaagtta attactatgt cgataaaatc gcggcagtag tcaaagaaga
    35101 aatcggactg gattaagaga tattttcata aaaaataaaa gaacaaggac cagtgtcaga
    35161 tgtctgacac tggtccttgt tcttttatta ttctactgtc actgatttcg ctaaattacg
    35221 tggtttgtca acgtcgaatc cccgtaatag cgttgcgtaa tatgcaagta attgagtcgg
    35281 tacgactgtg gttagagtag ttagataagg gtggacagtt ggtagcacca gctggtcact
    35341 ttccttttct aatccctctg agacaataac tagtgtttta gcgccacggc tttcgacttc
    35401 tttcaggttt cctcgagtat gcgcaccagt cacttcttca ctgatgattc caatcactgg
    35461 tgttccttct tcgatcaaag caatcgttcc atgtttcaat tcgccagcgg caaaaccttc
    35521 tgcctggata taactgattt ctttcaattt caaagcagct tcaagagaaa cgttgtagtc
    35581 agcgccacgt ccaatgtaaa aggcatttct tgtatcgctg agatatactt ctgccaactc
    35641 ttctaaataa tctttttggg cgatgaccga ttccatcgca ttagcaacta aactcaattc
    35701 atgagtgata tcgaatacaa gagaagtttc aaattctttt ttatcaccga tagctttggc
    35761 taataaagtt agtacagcta tttgtgcagt ataagctttc gttgatgcta ctgcgatttc
    35821 gggtccagca tgcaatagca gtgtaaagga tgcttcccgc gaaagagtag aaccagcaac
    35881 atttgtaatc gtaagtgaag ggtagccgag acggttgatt tttaccagaa cttggcggct
    35941 atctgctgtt tcgccacttt gactcaagaa aataaagaat ggtttttttg aaagaagagg
    36001 tgtgttatag ccaaattcac tggctaaatg aacctcaaca gggatctgtg tcaatttttc
    36061 cagagtctgt ttacctgcta accccgcatg gtaactagtt ccgcaagcaa cgatatagat
    36121 tcgatcgcta tcagacaatg tatcgaccag cttttgatca agagagacac gattgtcttc
    36181 tgttacgtat ttttgagcga tacgacgcat gataataggc tgttcgtcga tttctttcag
    36241 catataaaaa ggataggtac ctttctcgat atctgaagaa tctacttgtg cttcgaatga
    36301 agaacgttct acgatgtcac ctgcaaaatt ttgaataata actttttctg atgtgacggt
    36361 aaccatctct tcatcttcaa tctcaacgaa ttccttcgta tgagcaagca tagccattgc
    36421 atcgctggca atcacattga atccatcgcc gagtccgatt aatagtggac ttttattttt
    36481 agcgacgtaa ataacatcag gtgctgtccg atcaatcaaa gcgaaagcgt aagaaccttt
    36541 gatgacccgc aaagctttta aaaacgcctc ttgcgttgta gtatccttcg cgaaagtttc
    36601 aattagatga gcgataatct cagtatcggt ctcaccaata aagtggtcat ttgctaaata
    36661 agcaatttta agttcttcaa agttttcgat tacgccattg tgaacgagca cgaagcgtcc
    36721 actttgggaa gtatgtggat gggcgttttc ttcagaaggt tgcccgtgag ttgcccagcg
    36781 tgtatgacca atacctatat ttcctgtagt atcaatattt attttgtttt tcaaattttg
    36841 aattctgcct tgtgctttga ccagataatc agttgtgcca tctgagacaa agataccagc
    36901 tgaatcataa ccacggtatt ctaatttttc tagaccgtca atcaatcctg gtgttacatt
    36961 ttttaatcca atcattccaa caattccgca cataaaaaac atcctacttt ctataatgaa
    37021 ttcgtatgaa tgcttccagc tgcaggaaaa tccttttgtt ttatctttgc agatattttt
    37081 tgtggatttt ctgtagagtg cagtccctag ggccatccgc cgaatctttc gataaaccct
    37141 ctcctcgtca tctgtttaca cagacctggc gctatcctaa aaggtataac tatcctcctc
    37201 ttttgttttt tatctggaaa catttaaaat cataaccttt tgcgaatgtt cttgtcaaca
    37261 aaaaaagaat aaaaaataaa ttggtatata tcaaatgctt tgtcaaatgc tctttttatg
    37321 tcaaaaaata atgggttttt atctatcatc tttttcagga gttcggtaca atagaaagta
    37381 atcggataga ggaggagtaa aaatggattc aaaaattgag atcatgacac tgggaatgtt
    37441 aaaaaaacaa ttgtccaaat tcgaagcatc agctggtgta tcagatgata cgaaaatatt
    37501 tttagatact ggctgggata gcatccaaga aatcgcacct gatgccttgg aagttgctga
    37561 agctcgtgag tttacggtag aagatgaatt gacaaaagag tcgttttccg ggtatgcgcg
    37621 ggaagaaaaa gcagagcggt tcgacacttc tgagcagtca gaaactgtga tcgtcataaa
    37681 aaacttgtat tagaaaggag aaagaatgaa taagagacgt tgggcagcag ttctgattgc
    37741 tgcaggatta tttgtggttt cgttagtttc agctagtttg actaagcctc aggaaaacga
    37801 tcaaattgag ggattgaaca gttggttata tggggatgaa gaactagctt caaatgtgtt
    37861 ggaagaggga gacagtacgc aaaaaatcgt aaaattatca gtagatggaa cgattgctgc
    37921 gggaggggct tcaggtttat ttgcttctga aggttacaat cataaaaatt ttatggaaca
    37981 actgaaggcg attgaagaag atccaacagt caaaggtatc tttttagaag taaattctcc
    38041 tggtggcggt gtctacgaaa gtgcggaaat cgctaagaaa ttggatacga ttcgtaaaga
    38101 acatgatatc ccaatgtatg tcagcatgaa aaacatggca gctagcggtg gctactatat
    38161 ttctgctcaa gcagataaaa tctttgcaac agaagaaaca gtgaccggct caattggtgt
    38221 gatcatgtca ggattgaatt attcaggact tctagaaaaa ctgggtgtgg aagatacaac
    38281 agttaaaagc ggagcgttga aagatatggg ttcaggaacg cgaccagaga caaaagaaga
    38341 agaagcggtg cttcaagcat atatcgacag cgcatatagt cgttttgtag caatcgtcag
    38401 cgagggacgg aataaatctg aagaagaagt caaaaaaatt gctgatggaa ggatttatga
    38461 tggtgtccaa gcaaaagaag taggtttagt cgatgaattg ggatttcctg aagatgcttt
    38521 aaaagctatg cgaaaggaac aacagcttga agatgcggaa ctagtagaat attcttccac
    38581 gccatctggg tttgctagta catggttagg aagtaaacta gcagaattcc agggattgaa
    38641 gccttctgag actagtcaga tcttatccgt tcttgagaat ctagggacag cggaatcacc
    38701 aaaagcaatg tattattacg gaggtgagta agcatgtcag agctttcaaa tgaaaataag
    38761 gaacaagaaa aagataagca acagcagcct gctgtaggac ctgactccaa cttttcccaa
    38821 aaggtactga gctcttttga aagagagcct ttgacgcccg aagaaatcaa aaagaaacaa
    38881 cgtaaatgga aaaagtatac ggacacaaga aatgaaccgc caaaagcaaa tgattttcct
    38941 aagtatttct atgcaggttt ttttatccga atgtttgctt tcttggtcga tcttttgtgt
    39001 atcggagcta ttacgcgttt aacacttggt attgccaaaa atctaggttg gcttaatttt
    39061 tcagattctt atctgagtat ctacggattc acagccttac ttatttatct agcgtatttt
    39121 attttattga cgaaattcaa tcatggtcaa acgattggga aaatgatctt tggtattcga
    39181 gtcatcagct ttgatgaaga agaacttagc tggtcaaccg ttttagttag agaaggtgct
    39241 tgccggttca ttctaaaatt tcctttgctg atgttagggt atcttccagc aatctttaat
    39301 cagaaaaagc aacatctagg cgattttttc actaatacaa gtgttgttac gatcaatctg
    39361 atcaaagcat tcaaccagga agtcaacagc tgatgagcaa taaaaagcag taacagttga
    39421 aagcaccttc taaaagtcag atctaaaatc tagcttttag agggtgcttc agttattttt
    39481 aattgtcatg ctttttttgt tttgctacta ttgaacatga gctgaacatg agcaagagga
    39541 ggacactgcg tgaagccgat tgattttcca gaaaaatatg agaatttaat gcgagtagca
    39601 caacaagcac tagcaaacca gcaatatcag caggcaaaag aattatttca acgagcttac
    39661 gagttgaaag aaagctttga ggctaatagc ctgcttgttt tttgtcttta cgagttggac
    39721 gagaaaaaag aagcattgaa gcaagcactc ttgcatgaaa aacagtatct tcaaaatgag
    39781 gagtttgctg aattttattt tgacttgtta atcagtacac aagatttttt atatgctaga
    39841 aaattgatag caagtacaga tttttatgaa gctttcgagc agcgggtcat tgaaaaaatc
    39901 caatttgcag aagaattatc cggacaaatg gagaggcaga agatcaaggc actccacaat
    39961 aaatcggagg aacttccttc gttggaacct gcacgtcagc ttgcgtctat tgatgagatc
    40021 gaacagcttc cttatcatga gtttatccaa acagcgagta aacttattgt atcgccagag
    40081 gtccatattt tagcaagggc aaaattactg gagacactta ggcaattgaa cgaatgccat
    40141 cctgttttct atttgacgat tgaagaaaag ttagtcaaag tcataccaaa agatctgcca
    40201 aagccgcagc agcaggcaag ttaccagcaa ttatgtgtat tttcagatcg ttacggtaat
    40261 gaagatgcgc tgcttagttc cgtgttaaat gaagaattca cgttgcaatc tgcaattgtt
    40321 tatcctgtct atgatacata catcgaagac ccgaagcgct ggtttcaact gactgtagag
    40381 gcgtgtacag gaaaaacgat gtatgaaggt acagaagatg aaaaacaaga ttttttaaaa
    40441 aaaagagaaa aaattttgca gcaaatgatc ttcttccatt agattttatc agtgaaaaag
    40501 ataaaaatgt tatgaaaaac aaggattcta tccattcaat atgtttacaa tttgaaaggg
    40561 ttagtgtact atttaaaggt atgcatgaaa cgtttttgat gtataaaatg aatttctaaa
    40621 ctcggaggga attaaatgac tgcaacattt gaaaaaaaag gcacgaatga tggtgtttta
    40681 aaatttacga ttgcgcaaga cgaaatccaa aaaggattaa caactgcgtt taataaagtg
    40741 aaaaaaagct tgaacgtacc tggattccgt aaagggaaag tttctcgtca agtattcaac
    40801 cgtatgtacg gtgaagaagc tttatatgaa gatgcattga atgctgtatt accagaagct
    40861 tatgaagctg ctgtcaaaga agctggcatc gatcctgttt ctcaaccaaa aatcgatgtt
    40921 gaaagcatgg aaaaaggtca agactgggta atcactgctg aagtaacagt gaaaccagaa
    40981 gtaaaattag gacaatataa agatctagtt gttgaaaaac aagaccgtga agtaacagat
    41041 gctgacgtgg atgcacgtat caaacgtgaa caagaagcac aagctgaatt agttatcaaa
    41101 gaagatgctg cagctgaaaa tggcgataca gtagtaatcg acttcgaagg ctttgtagat
    41161 ggcgaagcgt ttgatggcgg aaaaggcgaa aattactcac ttgaattagg ttcaggctca
    41221 ttcattccag gttttgaaga tcaattagtt ggtcataaat caggtgaaag tctagatgta
    41281 aatgtaactt tcccagaaga ttatcaagca gaagatcttg ctggaaaaga agcagtcttc
    41341 aaagtgacaa tccatgaagt aaaagcaaaa gaagtgcctg aattagatga cgaattcgca
    41401 aaagatgttg atgacgaagt atcaacatta gacgaattga aagaaaaata tcgcaaagaa
    41461 ttagtagaaa caaaagaaaa agcagcggac gatgcaaaag atgaagcagc gatccgtatg
    41521 gctgttgaaa acgcagaaat cgttgaattg ccacacgtga tggttcatga tgaagtacat
    41581 cgttcaatgg acgaattctt gaacaacatg caacgtcaag gtatctctcc tgaaatgtac
    41641 tatcaattaa ctggttctac agaagaagac ttgcataaac aattcgaagg tgaagcagaa
    41701 atgcgcacaa gaacaaatct tgtgatcgaa gcaattgcag ctgctgaaaa tcttcaagca
    41761 acagaagaag aaatcaatgc agaaatccaa gaattatcag aaacatacaa catgccaatc
    41821 gaacaaatca aacgcgtgtt gacagaagat atgctggaac atgatgtgac aatgaaaaaa
    41881 gcaatcgaat tgatcacagg tacagcgaca gaagcgaaag aatagaaatc aagtaataaa
    41941 tcatttctga agaagctact tctagaaaat tgtttattcg aaataaggga ctgtgacacg
    42001 attttgtcac agttccttat tttttgttaa gagtaaaaaa tgcaaaggta taggtcagtt
    42061 gatcctttgt taaacacttt ttctttttac taaactatgt tatgatagta aaagactgta
    42121 aagtctttga tagtttcttg catacttttt gcaagttaag gggtgaagac catgtatgac
    42181 aatccaagta gcaatgagac cgtacgctgt tctttttgcg ggaaaaccca agaagaagtg
    42241 aaaaagatcg tagctggtcc aggggtctac atctgtaatg aatgtatcga cctatgtaaa
    42301 gaaatcattg atgaagaatt ttatgaagaa gctgtacgcg aattcactga agtccctaaa
    42361 ccactagaaa ttcttgaagt attgaataac tacgtgattg gacaagatcg cgcaaaacgc
    42421 tcgttagctg ttgccgtata caatcactat aaacgtgtca atcagcaagc acaagaaaac
    42481 acagatgatg ttgaactgca aaaaagcaat atctgcttga tcggacctac agggtctggt
    42541 aaaacatttt tagcacaaac attagcaaaa acattgaatg taccttttgc aatcgcagat
    42601 gcgacaagtt tgacagaagc tggttatgtg ggggaagatg tagaaaacat tttgcttaaa
    42661 ttgcttcaat ctgctgatta caatgtggaa cgtgcagaaa aagggatcat ctatattgat
    42721 gagatcgata aaattgcgcg taaaagcgaa aatgtatcta ttactcgtga cgtttccggc
    42781 gaaggtgtcc aacaagcctt gctgaaaatt ctagaaggaa ctgttgcaag cgtgccacct
    42841 cagggaggac gtaaacatcc acatcaagaa tttattcaga ttgatacgac gaacatattg
    42901 ttcatcgtag gtggtgcatt tgatggcatc gaaacgattg tgaagaatcg tatgggcgaa
    42961 aagacgattg gatttggtac acaaaaccaa aaactttctg aagacgaaag tgtcatgcag
    43021 catatcattc ctgaagattt actaaaattc ggattgatcc ctgagtttat cggacgatta
    43081 cctgttatgg cagctttaga gaaattgaca acagatgatc tggtacggat cttgactgag
    43141 ccgaaaaatg cgctagtcaa acaatatcaa aaacttcttt ctctagataa taccgaactt
    43201 gaattcgaac ctgaagcgtt gagagccatt gcgaaaaaag caatcgaacg caataccggt
    43261 gctcgaggac ttcgttccat cattgaagaa atcatgatgg atgtgatgtt tgatatccct
    43321 tcaaatgaaa acatcgaaaa agtgattatc acaaaagaag cagctgaatt gtctggtgag
    43381 ccaacagtaa tctataaaga ttcagagaaa aaagcgggtt gatgaaaaga ggctgccgta
    43441 aaagtcattt tggcttttgc gacagccgct tgctttattc atcaccttgt taaggagaga
    43501 gaccatgaaa gtccatcaag ctgaaattat cataagtgca gtggcaccaa aacaatatcc
    43561 agatacacaa ttgccagaaa tagctctggc aggacgatca aatgtaggga aatcttcttt
    43621 tatcaacaca ttgattgatc gaaagaattt agcccgtact tccggtaagc caggaaagac
    43681 ccagacattg aatttttatt tgatcgagaa cgcattacat tttgtcgatg ttccgggata
    43741 tgggtatgca aaagtatcca aaactgagcg agcaaaatgg ggaaagatga tcgagacata
    43801 tttaacgact agagaccagc tacgtgcggt cgtttcttta gtagatttgc gtcataagcc
    43861 ttctcaagaa gatatccaga tgtatcaatt tttgaagtat tatgatatcc ctgtaattgt
    43921 tgttgccact aaggcagata agatccctcg tggtaaatgg aacaagcatg agagcgaaat
    43981 caaaaaagcg ttggattttg atcgcaatga cacgtttatt ctcttttctt ccgttacaaa
    44041 acagggaaaa gaagaagcgt gggcagcaat cgaacaagca attgaatcat aaaaagaata
    44101 aaaaagactt accagtcaaa ctctttcata aaagtttggc tggtaagtct ttttttgagc
    44161 ttaacttact tttgtttctt gtcttttttc ttttcatttt gtggctgtgg ttttagaaat
    44221 cgatcgatct cttcttttgg tgcaactgtt ttttccggat cgacagcaaa tgattcgaaa
    44281 agtttatcta aattatctgt acgttcaaat ttcaacccca ttgtaaacac ctctttgttt
    44341 ctttttactt agtctatcat gtaatcgtcc aaattggttt ctaagacata aagttttagt
    44401 ttttctttat cgttggttat aattaaatta taaaaagatt ttaaagaggt gagaaaatga
    44461 ttggcgaaga taaattattg atgactatcc agctcacaga cttaaatgaa gaagaaaaaa
    44521 aacaggaaat aagcgattgg gctattttga cacgtctgtt gatccagcca acatttatcc
    44581 gctctttcac acaacaagcg gatccgtatg atcttcgaaa gcttcaagaa aaaataatgc
    44641 tagcatcggt aagagacgaa agttggttag ttgtaggaaa tgacgagaac gaatgtagtt
    44701 ttcgcttgga agataatcaa ttattgataa aaaatgtatt gtctatctct gttttcaaag
    44761 aaaagcaatt tttgattcga gacttcattc agaaaaaaat gattgctcat ggggtgttcg
    44821 cctatatgcg agcttatagc gagtttatct atcacaatac aaaacagatc tctcaacggc
    44881 tattgtttga gaaaaaggat gaaatcgaac atttacctaa aatgaaacaa caaaacgggg
    44941 aagtagtagt cgattgcaac caatttccag gatacgatct tttttaccaa ggtttatgtt
    45001 tcacttcgtg ttgggaaatg tattatagtc gctattatca tcaaatcatc cctaaaccga
    45061 tttttctcga catccaacaa gtagaaaaag tgaaggaatt agaaaacgaa gtcatatgta
    45121 tccagcttta tcgagacccc tttaactggc aaaagccaaa caatcaaatg tttcaatcct
    45181 attttaggga ccagcttggg tttgatcatc ttgcttggga caatggtgtc ggtttattga
    45241 aaccgccatt cgttgaatac atttacactg accatacgat ccaatctgtc cagtatcaga
    45301 atgcacaaat gcaacctgtt cctaaaaaga atgcttcatt ttttgtaaca aaaagttatg
    45361 atattcaaca aggagattat aaagaaaggc gtgttcgcgg aacactaaat gctcaggcgt
    45421 attttccttg ggttgacgag aatcgctcgc gcatgatgtg ctataaagtt atcgatccga
    45481 ctgttgcttt ggataatgga atcgaagctt attgttatta tattagggaa tatctagaag
    45541 tagaggtaac agatgaaaaa tatcaagagt atcttttatc cttaagaatc tatgttcctt
    45601 cagaatccct gtcagaatta ccgttaaagg aaatcaaaca tagactctct gatgttacct
    45661 tcaaacgaat aaagaaaaag cggcgaagta tccaattcga tgtcaagaaa ggtgagcagc
    45721 atctgcgtgt gcaattttta gattaccgag aattagaaca attgattact ttacaaaaaa
    45781 tatgaggtga agaaaatgga taaacaactc tattatcgaa taatggttcc ggcatcatta
    45841 ttatccgtct gtctcttttt tgttcctaat tttcctattc gatcagtcat cggttttttg
    45901 gtcatgtttg gcgggatggg actttattat tatcttcttc a
//
LOCUS       DAZUMI010000007        44751 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000007 DAZUMI010000000
VERSION     DAZUMI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.007"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1008)
                     /locus_tag="ACG81C_000299"
     CDS             complement(<1..1008)
                     /locus_tag="ACG81C_000299"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5063493.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="ACG81C_000300"
     CDS             complement(1188..1532)
                     /locus_tag="ACG81C_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5063494.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="ACG81C_000301"
     CDS             complement(1525..1959)
                     /locus_tag="ACG81C_000301"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5063495.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="ACG81C_000302"
     CDS             2097..2834
                     /locus_tag="ACG81C_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063496.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="ACG81C_000303"
     CDS             2827..4038
                     /locus_tag="ACG81C_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063497.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4242..5024
                     /locus_tag="ACG81C_000304"
     CDS             4242..5024
                     /locus_tag="ACG81C_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5063498.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="ACG81C_000305"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="ACG81C_000305"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5063499.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACG81C_000306"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACG81C_000306"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5063500.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="comGA"
                     /locus_tag="ACG81C_000307"
     CDS             7740..8669
                     /gene="comGA"
                     /locus_tag="ACG81C_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5063501.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /gene="comGB"
                     /locus_tag="ACG81C_000308"
     CDS             8626..9675
                     /gene="comGB"
                     /locus_tag="ACG81C_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5063502.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /gene="comGC"
                     /locus_tag="ACG81C_000309"
     CDS             9672..9950
                     /gene="comGC"
                     /locus_tag="ACG81C_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5063503.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /gene="comGD"
                     /locus_tag="ACG81C_000310"
     CDS             9925..10395
                     /gene="comGD"
                     /locus_tag="ACG81C_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5063504.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10367..10714
                     /locus_tag="ACG81C_000311"
     CDS             10367..10714
                     /locus_tag="ACG81C_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063505.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10677..11102
                     /gene="comGF"
                     /locus_tag="ACG81C_000312"
     CDS             10677..11102
                     /gene="comGF"
                     /locus_tag="ACG81C_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5063506.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /gene="comGG"
                     /locus_tag="ACG81C_000313"
     CDS             11074..11433
                     /gene="comGG"
                     /locus_tag="ACG81C_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5063507.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="ACG81C_000314"
     CDS             11605..12612
                     /locus_tag="ACG81C_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5063508.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="ACG81C_000315"
     CDS             12637..13821
                     /locus_tag="ACG81C_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5063509.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACG81C_000316"
     CDS             complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACG81C_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5063510.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15946..16074)
                     /locus_tag="ACG81C_000317"
     CDS             complement(15946..16074)
                     /locus_tag="ACG81C_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063511.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16419..16562)
                     /locus_tag="ACG81C_000318"
     CDS             complement(16419..16562)
                     /locus_tag="ACG81C_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5063512.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="ACG81C_000319"
     CDS             complement(16785..17264)
                     /locus_tag="ACG81C_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5063513.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="ACG81C_000320"
     CDS             complement(17337..17621)
                     /locus_tag="ACG81C_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063514.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="ACG81C_000321"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG81C_000321"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="ACG81C_000322"
     CDS             complement(17965..19251)
                     /locus_tag="ACG81C_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5063515.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="ACG81C_000323"
     CDS             19389..20060
                     /locus_tag="ACG81C_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5063516.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="ACG81C_000324"
     CDS             20026..20514
                     /locus_tag="ACG81C_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5063517.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="ACG81C_000325"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="ACG81C_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5063518.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="ACG81C_000326"
     CDS             21475..22239
                     /locus_tag="ACG81C_000326"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5063519.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="ACG81C_000327"
     CDS             22472..24685
                     /locus_tag="ACG81C_000327"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5063520.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /gene="dtd"
                     /locus_tag="ACG81C_000328"
     CDS             24696..25142
                     /gene="dtd"
                     /locus_tag="ACG81C_000328"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5063521.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="ACG81C_000329"
     CDS             25313..26572
                     /locus_tag="ACG81C_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5063522.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="ACG81C_000330"
     CDS             complement(26661..27608)
                     /locus_tag="ACG81C_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5063523.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /gene="hisS"
                     /locus_tag="ACG81C_000331"
     CDS             28002..29294
                     /gene="hisS"
                     /locus_tag="ACG81C_000331"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5063524.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="ACG81C_000332"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="ACG81C_000332"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5063525.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="ACG81C_000333"
     CDS             31187..32062
                     /locus_tag="ACG81C_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5063526.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="ACG81C_000334"
     CDS             32328..34211
                     /locus_tag="ACG81C_000334"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5063527.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="ACG81C_000335"
     CDS             complement(34286..34570)
                     /locus_tag="ACG81C_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5063528.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="ACG81C_000336"
     CDS             complement(34560..35294)
                     /locus_tag="ACG81C_000336"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5063529.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="ACG81C_000337"
     CDS             35423..36046
                     /locus_tag="ACG81C_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5063530.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="ACG81C_000338"
     CDS             36238..37359
                     /locus_tag="ACG81C_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5063531.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="ACG81C_000339"
     CDS             37366..40494
                     /locus_tag="ACG81C_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5063532.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="ACG81C_000340"
     CDS             40513..43707
                     /locus_tag="ACG81C_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5063533.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="ACG81C_000341"
     CDS             complement(43853..44560)
                     /locus_tag="ACG81C_000341"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5063534.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14356 a         7952 c         9396 g        13047 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgag aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggt
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga a
//
LOCUS       DAZUMI010000008        43042 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000008 DAZUMI010000000
VERSION     DAZUMI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.008"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..3375
                     /locus_tag="ACG81C_000342"
     CDS             97..3375
                     /locus_tag="ACG81C_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5063535.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3916..5823)
                     /locus_tag="ACG81C_000343"
     CDS             complement(3916..5823)
                     /locus_tag="ACG81C_000343"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5063536.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5953..6828
                     /locus_tag="ACG81C_000344"
     CDS             5953..6828
                     /locus_tag="ACG81C_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5063537.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6908..7882
                     /locus_tag="ACG81C_000345"
     CDS             6908..7882
                     /locus_tag="ACG81C_000345"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5063538.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8062..9207
                     /locus_tag="ACG81C_000346"
     CDS             8062..9207
                     /locus_tag="ACG81C_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5063539.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9344..9688
                     /locus_tag="ACG81C_000347"
     CDS             9344..9688
                     /locus_tag="ACG81C_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5063540.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9959..11245
                     /gene="efmA"
                     /locus_tag="ACG81C_000348"
     CDS             9959..11245
                     /gene="efmA"
                     /locus_tag="ACG81C_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS5063541.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11383..11982)
                     /locus_tag="ACG81C_000349"
     CDS             complement(11383..11982)
                     /locus_tag="ACG81C_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5063542.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12386..13507
                     /gene="mnmA"
                     /locus_tag="ACG81C_000350"
     CDS             12386..13507
                     /gene="mnmA"
                     /locus_tag="ACG81C_000350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5063543.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13753..15174
                     /gene="arcD"
                     /locus_tag="ACG81C_000351"
     CDS             13753..15174
                     /gene="arcD"
                     /locus_tag="ACG81C_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5063544.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15188..16306
                     /locus_tag="ACG81C_000352"
     CDS             15188..16306
                     /locus_tag="ACG81C_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5063545.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16366..17658)
                     /locus_tag="ACG81C_000353"
     CDS             complement(16366..17658)
                     /locus_tag="ACG81C_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5063546.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17808..18368
                     /locus_tag="ACG81C_000354"
     CDS             17808..18368
                     /locus_tag="ACG81C_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5063547.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18497..18874
                     /locus_tag="ACG81C_000355"
     CDS             18497..18874
                     /locus_tag="ACG81C_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5063548.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18807..20879
                     /locus_tag="ACG81C_000356"
     CDS             18807..20879
                     /locus_tag="ACG81C_000356"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5063549.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21090..22037)
                     /locus_tag="ACG81C_000357"
     CDS             complement(21090..22037)
                     /locus_tag="ACG81C_000357"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5063550.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22283..22747
                     /locus_tag="ACG81C_000358"
     CDS             22283..22747
                     /locus_tag="ACG81C_000358"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5063551.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22809..23852)
                     /locus_tag="ACG81C_000359"
     CDS             complement(22809..23852)
                     /locus_tag="ACG81C_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5063552.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24229..25194)
                     /locus_tag="ACG81C_000360"
     CDS             complement(24229..25194)
                     /locus_tag="ACG81C_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5063553.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25428..26273
                     /locus_tag="ACG81C_000361"
     CDS             25428..26273
                     /locus_tag="ACG81C_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5063554.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26464..27651
                     /locus_tag="ACG81C_000362"
     CDS             26464..27651
                     /locus_tag="ACG81C_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5063555.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27657..28013
                     /locus_tag="ACG81C_000363"
     CDS             27657..28013
                     /locus_tag="ACG81C_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5063556.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28015..28353
                     /locus_tag="ACG81C_000364"
     CDS             28015..28353
                     /locus_tag="ACG81C_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5063557.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28429..28685
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28748..29782
                     /locus_tag="ACG81C_000365"
     CDS             28748..29782
                     /locus_tag="ACG81C_000365"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5063558.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29775..30461
                     /locus_tag="ACG81C_000366"
     CDS             29775..30461
                     /locus_tag="ACG81C_000366"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5063559.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30485..31333
                     /locus_tag="ACG81C_000367"
     CDS             30485..31333
                     /locus_tag="ACG81C_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5063560.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31530..32303
                     /gene="sufC"
                     /locus_tag="ACG81C_000368"
     CDS             31530..32303
                     /gene="sufC"
                     /locus_tag="ACG81C_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5063561.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32316..33602
                     /gene="sufD"
                     /locus_tag="ACG81C_000369"
     CDS             32316..33602
                     /gene="sufD"
                     /locus_tag="ACG81C_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5063562.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33599..34834
                     /locus_tag="ACG81C_000370"
     CDS             33599..34834
                     /locus_tag="ACG81C_000370"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5063563.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34821..35291
                     /gene="sufU"
                     /locus_tag="ACG81C_000371"
     CDS             34821..35291
                     /gene="sufU"
                     /locus_tag="ACG81C_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5063564.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35296..36687
                     /gene="sufB"
                     /locus_tag="ACG81C_000372"
     CDS             35296..36687
                     /gene="sufB"
                     /locus_tag="ACG81C_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5063565.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36782..37924)
                     /locus_tag="ACG81C_000373"
     CDS             complement(36782..37924)
                     /locus_tag="ACG81C_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5063566.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37963..38697)
                     /locus_tag="ACG81C_000374"
     CDS             complement(37963..38697)
                     /locus_tag="ACG81C_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5063567.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38929..39204
                     /locus_tag="ACG81C_000375"
     CDS             38929..39204
                     /locus_tag="ACG81C_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063568.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            39302..41299
                     /locus_tag="ACG81C_000376"
     CDS             39302..41299
                     /locus_tag="ACG81C_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063569.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41375..41710)
                     /locus_tag="ACG81C_000377"
     CDS             complement(41375..41710)
                     /locus_tag="ACG81C_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5063570.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41838..42052
                     /locus_tag="ACG81C_000378"
                     /pseudo
     CDS             41838..42052
                     /locus_tag="ACG81C_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            42089..42451
                     /locus_tag="ACG81C_000379"
     CDS             42089..42451
                     /locus_tag="ACG81C_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5063571.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42497..42823
                     /locus_tag="ACG81C_000380"
     CDS             42497..42823
                     /locus_tag="ACG81C_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063572.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        14113 a         7683 c         9023 g        12223 t
ORIGIN      
        1 atacacttat tataattttg ttttttattg acagtaaaaa taaattaggt aatactttaa
       61 gtaaacgctt tctaatttgt tacgggagga atacacttgt taaaaaaacg tataaaatac
      121 agtgcgctaa tgatgaatct ccttatgctc agtaccccgg ttttaatttc tgtcgttgaa
      181 gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg cacacgtgaa cattgaaggg
      241 atgtcaacaa tggaggaaat accaagaact agtgaagata tggatacgga agaaggaaat
      301 gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa agcaatctga agaagcaaat
      361 gaagagtgga aaggggttgt tttcggagaa agcacaagtg ctagtaggta tgccattgaa
      421 ccattagaag acggcgtgcg gctctcttcc acgaataacg gcggaaaatt ccagtcaagc
      481 ggatcagatg gaatgaccta ttattatcaa gcaatcccca aagacatcaa ctttaggatg
      541 cgggcgacga tcgaaataga atcttggacc tatacgaatg cccaagaagg atttgcttta
      601 atggtaagag acgcagtacc aaaaaatcat ttgtttgggc aggcatttta ttcaaattca
      661 tttgccatac taggcagtcg gatcgaatat gtctgggact cggatagaca agaagtggtc
      721 aacacgagcg gcagcaaata tacgatgcga cttggattgg ggacacgggc aatcacagga
      781 atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt ggaaacaaca
      841 cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa tatattcgga
      901 aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga cgtagagatg
      961 aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg agaagaaatg
     1021 gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc ggttatttat
     1081 gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga agttgcttac
     1141 tcagatcctg cgacagatcc tcctgggcaa gaaagaccta ctcgcttgat tgatccgatt
     1201 tacactgtga cctctagtaa tcattcagga aaccaagatc atactatgtc ctttagagca
     1261 aatgctgacg gattattgac aattaaaaat aaagcaacag gagaggtgct taagaacgca
     1321 aaaaacttgg ctatcactgc gaatcgggaa tttgattatc agacaaaact gatggaagga
     1381 acgaacgaat ttactttcag ttttacgcca gataaaaact atccgccaga agaatacgta
     1441 gagatgacta gttatgaaac aaaagagatt cttcatacag tggactaccg taaacctaaa
     1501 tattcgacag tttatgtaac accagaaggg tcagatgcag gaaacggaag cagacaaaat
     1561 ccattatctc tgacacaagc attgagatac gcatatgctg ggcagacaat cgtcatggcg
     1621 cctggaactt attccttcga aagaggattg acgattccaa aaggcgttaa cgggacaaga
     1681 gaaaacccga ttcagttgat tgcagagaaa aatccagaaa acaagcgtcc tgtacttgat
     1741 tttaaaggaa ctggaaatgg gatgacactg ttcagtcact actggggttt acgagggttt
     1801 gacattacaa atagtgcagc tatgcaaaaa ggattacaga tttctggaaa tcataatttg
     1861 atcgaagaga ttcatgctta tcggaatgga aatacaggaa tccaaatcag cggtagctca
     1921 aatgatcctt tcgaagcatg gcccgcgcat aacttagtca agaactgtac atcttttgaa
     1981 aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac aataggagaa
     2041 ggaaatgtat ttgacggttg tatcgcgtat cataatgcag atgacggttg ggaccttttt
     2101 gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca aaaactcagt tgcttatcga
     2161 aacggctggg taccaggaat tgagggagaa ggtaacggta atggattcaa aatgggcggt
     2221 tcttctttaa cgggccctca tgaattgatc aatagtttgt ctttcgaaaa tcttgcgaaa
     2281 ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag ttttaataac
     2341 ggttcatata atgtggctct ttatacctct tcagcaggaa acacagacta ccatgcttct
     2401 ggtatcttat ctttccgaac agagaatctt gagatgagag agcagatccg cccattgaat
     2461 caaaacgaag atcaaatcta ccactcgttg aactactatt ggaatgaacg agagaaacaa
     2521 tcagaaaata ctcttggaca taccatatca aaagattgga tcgagtctct aaatacatgt
     2581 tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg gaagtatcca tgttcatgga
     2641 ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg aaagagtgga tggtcttcca
     2701 ttatctgtag gtgctatttt tgacggagaa acctcaccgt cttcggaata tccagagtat
     2761 gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc gattattata
     2821 caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact gaagcctttt
     2881 acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga agatatgcgt
     2941 tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt ttctaatcat
     3001 accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac ttatcgttcg
     3061 gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca gcaaaaagcg
     3121 atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga aagagaaaat
     3181 ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac cttctcatcc ggataggaca
     3241 gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa aaacgggaga atttaataac
     3301 ccgttgctag cattagctgg aggaattttg ttaatcggtg tagttgttta tgtcatgaaa
     3361 cagcgaaaaa aataagtgga atttctcctt ttatgatgta aaacaattga ctgaggaaag
     3421 taaaaataga aggaaatgag accaatagat gtctcacttc cttctatctt tttatagatg
     3481 aggttctcct tgctgctttt gattggctgt tcttattact tgatgcagga cagctttttc
     3541 acaatctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct caacaaacgg
     3601 caaaatatga ggaactattt ttccgttttt ttctctaata ctcaggtcag gacgctgatt
     3661 tcacaacctc tcgataaacg gtgttcaaaa gccagtttct cggtagtaag tcaaatttat
     3721 gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tacttgaaac taagcagctt
     3781 tttcacaacc tctaatacat ggtattccag aagtaacttc ttcgaaaaat aagccgaaat
     3841 gtcacaaaaa tttgagaagc gattttccca aattccttct tatttcttga agctgaccac
     3901 ttctggcaca acctcttacc agattcgcac tcttttttct ggcgctagat acatagcgtc
     3961 ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg ttttttggtt gcacgtttgc
     4021 tcggagttta gcaggggcat ggacatcgat ttgcagtaaa agttgttggt attctttttt
     4081 tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat tcctcgatag aaagatctga
     4141 ttccgttttt gctgcttcca atgcacagct tagaccgcct gcatcagcaa tattttccga
     4201 aacggttaat tttccgttta cttttccgtc agcaaaaggc agaccatcga attcttcgat
     4261 cattgcttgt gctttgtttt gaaattgtgt aagatcttct tctgtccacc agttgttcaa
     4321 gttgccatat tcgtcaaata aagcgccgtt attatcaaat gcatgggaaa tctcatgagc
     4381 gataacagct ccgattccac cataattagc agagcttgat tgttctagac tgtaaaatgg
     4441 tgcttgaagg atcgctgccg gaaagacgat cacattccga aatggatgat aataagcatt
     4501 gaccgtgtca gcactcattt cccattctga acgatccact ggtttgttcc atttgctgaa
     4561 acgatctttt aatgtgagtt tgctgaaaac aagagtattt gaaagcaaag taccaccctg
     4621 atctgcaggt actgttttga actggtcgaa taatgctggg atcttatcgg ggtagcctac
     4681 ttggatacct aagttattta attttatgac ggctttttct tttgttgcat ccccaagcca
     4741 gtcattttct tctagacgtt ttttataaac ggcgatcatt tttttcacca tttgttctac
     4801 gtctttttta gcagcttcgc caaaatattt ttttccgtag taatcgccaa ctacttgatc
     4861 gaattgtcca gaggcaaggt aatacgctgc ttttttctga ggcattgctt cgtctgttcc
     4921 agacaatacg cgagaataga ttccgctgac ttggcggaat tcttctgata gataaccact
     4981 aagagaacgt actgttttaa tgatcatcca gcttttgaat aacggaaaat gagcttctgt
     5041 caggatgtca ttcaaatgat cgaagtacac tggatcagtt acgatcactt tatctggagt
     5101 tgtcccaatc aaacccgtga caagtttttt caagtccagt ttatctgtgt atttaataaa
     5161 tgtatcaaaa gattgcggat tatacatttt gctgtaatct gcgttttctt ctgcactttt
     5221 tacatgagga gcaatcaatt tatcaaattg cactgcttgt tccgtgattg cttctgcctg
     5281 acttttttct ttgccggcaa gctctaataa ctgaacagcc atatcaaaga aaacgcctat
     5341 tagttgtgca ccgttgggat ggtctgatgc ataatacgtc ttatctggaa ggaataaaga
     5401 aggcggataa gcaaagaagg cgttcgtttg cgcattcttc atatctgcat ctacatccag
     5461 attaaatggc aatggtaatg tatccaacag ccattctgga aattgcttat tcaattcgtc
     5521 gaaagactgc aatgattcaa ctttttcgat tataggttga agcggttgag catgcaactc
     5581 atttcttcgg tcataatcgt ttgccagttg ataatatgca aggaaatgtt tcattttttc
     5641 tgaagacact ttttctggat gggcaagcag atcgtctgtt tctcccatca aaagttggtc
     5701 gatcccgtca actaaatctt ggaaaccgcc tgtagctggt ttatctgcag ggatttttgc
     5761 tgttttttgc cattcttcat tgactgcttc atagaaatct tgtttcaatc gttctttgtt
     5821 cattcatcga tcgctccttt tttgtttcgt gtgcccatta tcttacaaaa tgaaggaatt
     5881 aacaaataga acataaaaaa ttgaaaattc gaacaatcaa actgatttgt ttttttcaat
     5941 ccttcactta taatgaaaaa aaggaggcga tcccaactga atcaagtagt ggataaacct
     6001 ttatatttca aagtagataa aaaaattaaa aaattagcca gcactcagca gctgcagtca
     6061 agaaaaagta aattgctttt tttagctctg gtttttgaag atcaaagtta tgtgattatc
     6121 gatcaatcag gacatccggt agaatattct ccagcaaaat atacttatca agagggtctt
     6181 tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg agttatctag atggatcaac
     6241 agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt caggaaagga actcgcaaat
     6301 tgctgggtgg gattgccaga agaacgtttt ttgaggtata agaaatgggc taccccctca
     6361 ggctatcttt gtggtactta cgcggcagca gttctccttg cctattatca agactatcgt
     6421 aaggggtgga tgcttccaaa tgagattcgc aagaaaaata cggctgattc gttggttttg
     6481 acgaaggcat tgcgtagcca gatccaacca ctaggacttc ctacgattcc ttttcaggtt
     6541 agtacaggga tttctagttt tttaaagaag aatggcaatc atgagcgtgc acgagcaact
     6601 cttctaggtt catggcagcg agctacaaaa cgtatacgtg aaggcaaacc tgtgatgatt
     6661 ggtattttga aagtacttgg cagtacatat ggcaatcatt gggtgacagc gtacgcttat
     6721 tttgaaactg aaacaggaga acgttattac aaagtacacg acaactgggg cgattatcat
     6781 aaagtcattc ctgccagttg gagtaacgga acagtttcac ttccttaaga atttgctgaa
     6841 attaacagaa aatcttggaa aaatatgtta aactatcgaa gaatagagaa gttttgggga
     6901 ggactacatg agtaataagt atcaggatga cacgttgaag atttttagtt tgaatggcaa
     6961 ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca gaattaggta aaagcgtcgt
     7021 gaagcaattt agtgatggcg agatttccat caatatcgaa gaaagtattc gaggggatca
     7081 tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat tactatatgg aactactgat
     7141 catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc aatgtcgtat tgccttacta
     7201 tgggtatgct aggcaagacc gcacggcgaa acctcatgaa ccgatcactg cgaaattgat
     7261 cgctaacttg atcgaagaag caggggcaac tcgagtactg actttagatc tgcacacggt
     7321 tcaagtacaa ggattctttg atattcctgt agataattta tttacgatgc cgttgtttgc
     7381 tcattattac cgtcaattag gtctgacggg agatgacatc gtagttgttt cgccaaaaaa
     7441 cagcggtgtt cagcgtgcac gtagtctttc agaatatttg aacagcacat tagccatcgt
     7501 agatcatgca gatgatgctt caactgaggg tggttatgtg atcggtgatg ttcaaggaaa
     7561 gacatgtatc atggtagatg atatcttgaa cacaggagca acgcttgcac gagcagcaaa
     7621 tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt gcgtcacatg gattattgtc
     7681 cgatcatgcg aaaagtattt tagacaatgc accaatcaaa gatatttgta tcactgattc
     7741 tgttttcaca gaagaaaatc gtcatccaga aaacttaaca atcgttacat gttcagaatt
     7801 aatgggtgaa gcggtcaaac gcatccatga aaatacaccg atgagtccgt tattccgtct
     7861 ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa gaagttgtga cagaagtgag
     7921 cagcttcaag gagctgcgac aagacttttg tcacagctcc ttttttctat gcccatgaaa
     7981 cgtcctctag acaaaaatga gctcttcttt ggtttgttcg ctattttatg ctagaatgtg
     8041 cctagcaata atttggaagg gatgaatgaa gtgaaaacta tttatctgga tcatgcggca
     8101 actaccccca tgcatccaca agtaattgaa gcgatgacgg caatcatgca agacacgttt
     8161 ggaaatcctt caagcataca tggatttggt cgccacgcgc atgaaaaatt ggaaacagca
     8221 agacaaatca ttgcagacag tctgcaagca aaacctcacg agatcatctt taacagtggg
     8281 ggaacagaag gggataatac agctatctta gaaacagcgt tctcgcgtca aaaagaaggc
     8341 agacatctaa tcactacagc tatcgaacat cctgctgttc ttaataccat gaaatattta
     8401 gagacaaagg ggtttgacgt tacatatctg cctgtagatg aaaaagggaa tctgtcgatc
     8461 gaagaggtca aaaaagcgtt acgagaagat acgatcttgg tctctgtgat gtacgggaac
     8521 aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg aacttttagc ggatcatcca
     8581 gcttatttcc atacagatgc ggtacaagcg tacggaaacc aaacgatcca tccagaagaa
     8641 ttaggaatcg atttattaag tatctctgct cataaaatca atgggccaaa aggcgtcggt
     8701 ttcttatata aacgagacgg agtaaatatc cctgcattgc ttttaggcgg ggagcaggaa
     8761 gaaaagcggc gtgcaggtac agaaaattta gcagggatct ggggtatggc aaaagcagtt
     8821 gaactgctgt cacctgaaga acgtgaagca cgtaacaaca aatataaagg attccagcaa
     8881 ttgattcttg atacattaga agcaaacgaa atcccttatc aagtaaatgg tgatttaacg
     8941 aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg aagcaaatga tttgctactg
     9001 atgaagcttg atcttaatgg ggtcgctatt tctactggtt cagcttgtac agcaggaaat
     9061 atcgagcctt cacacgtatt agaagcaatg tatggagaag atacaccgat actaaaagaa
     9121 tccatccgta tcagctttgg tttgggaaat accgaagacg acatccagta tttccttgat
     9181 caattagttc gtgctgtcaa aaaataacta gtgaagtcat tcttatatga acagatgtct
     9241 gagagaacgc cttcattttt tagggaactt aactgaaaaa cctagtaaaa atcaggataa
     9301 acttttataa taaagagaaa ctaattttat cgaggtgaaa atcatggcat ttgaaacaac
     9361 agcaacggta ctgggcgcac caattagcta tcgtttgcat cctgatgcaa aaagatatac
     9421 attgcgcgat aatggattta cagaaacaaa cggaggcaat tatcaattga tccgaccatt
     9481 agatgccacg ccgcaaagca aagaaggatt caaattaaaa atcactgtat caaaagatat
     9541 tcaaacattg aaaatgtcga tcactacagc aaatggattg aaagcagtca atattttcaa
     9601 agatccaaaa cagaaaatga gccaagataa attttatttt ctgatggatg ggatgattag
     9661 ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata aaaagacaac tagttttgaa
     9721 acggcctatt gttagatgac taactttgta aggacgacgc aaattagttg tcttttttat
     9781 ttttaaattt aacacatgtt gttattttta atattttgct gatttattgt ttctaagtaa
     9841 agaaatttaa attgaaacga ttgttattta gggaaaaagg tattttttga aaaaagaatc
     9901 ccgtataatg attgttgtgc aaacgctata caaaatgaaa aagaaaagag ggatgtttat
     9961 ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga aattatttgt tctttttatc
    10021 gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa tatgcgatca tctggtattt
    10081 gacaagagaa accggttcgg cgacaatttt gagttttgct actttactag gaatgattcc
    10141 catggtttta ctaagcccat ttgtaggacc gctagtggat cgctgggata aaaaagctct
    10201 tttgattgtc acggatatta ttgtagcgat tttcgcgctt attttagcag tagtcggaac
    10261 gatttcagaa tccttcccga tttggctagt ttttgtgtct ttgtttatgc gttcggtcgc
    10321 acaaacattc caaatgccaa cgatccagtc gattatgccg acaatcgttc catcttctca
    10381 cataacaagg acgaatggac agttaggaat ggtccaatca gcgaatttca tcattgcgcc
    10441 agctctaggt gcggctttgt tttcagtcgt tcctgtcaat taccttattt tattagatgt
    10501 attgggggca gtttttggtg tcggcttatt gatttttgtg aagatcccaa aagtttctcc
    10561 tgaaatattg gaagttccgc ttactatttt taaagatgca aaatttgggc tgcagcaatt
    10621 gatggataat aaagggttat ggtacataac gatcaatggt gcatttgtga tgctgttgtt
    10681 catgccagca atcagtctgt atccattgat gacgctagat tactttggtg gttccgttgg
    10741 acaggcggga gcagtggaag tagtctacgc agtcggaatg ctcttaggcg gtgcactgat
    10801 cagttttata ggaacatgga aagaccgaat gaaacctatt attatagcct acatcattat
    10861 gggcctgacg atcggtgcaa gcggattggt tccaaacgat agtcaagggt tcttgtactt
    10921 tcttatcttg aatgcaggag caggttgtgc aacgccatat ttcaatacac tgctgatggc
    10981 aatgatccag cagagttatg aatcgaatgt tttaggtcgt gtcttaggga acttcaactc
    11041 tttgatgaac ctggcaggtc caattggatt attatttgca ggaccattag ctgatcgttt
    11101 aggggtagaa aaaatgttct tgttttccgg aatcgggatt ttattatgcg gaatcgtttt
    11161 atttttaacg tcagctgccc gaaattatga taaagaatta cagaaaaaac tggtaaaaga
    11221 acaccatgag caaaaagacg aatagtatgg aatggagttg tgacagaagt cttgttacaa
    11281 ctccatttct gatcctaatg ttcctgtggc aataagccta ctcaaatgaa aaatatgagt
    11341 aattatcttt atttgtttaa tacttgatat agggcagttt tttcacgacc tatcgtactt
    11401 cctctttgct tcatagattt ctttgatttg ttgctcagat aacggagtgc caaatggtag
    11461 tttttccttt aagagttctc tttccggtgc gtctacgcct acattgacat cgttctcgat
    11521 aggtacgctg taatgatgga tatgtccatg aagattgcgg atagtgtcgt taggcccgag
    11581 caacatcgga taatgagtca agtaatattg ttgatggtcg aatttaacga tggcacctac
    11641 atcataaaaa taaaatttgg gacttccgtc agtaagccta ggatcatttt tttctaaata
    11701 acggaaaaaa gcgcgactgt catggttccc tttgatcaag tggattgttc cttgcagttg
    11761 agtgaccagt tccaaaattt ctgcatttgc tttttcatat tgcggatgca tagcaaggtc
    11821 gcctagatga taaacatggt cggtttcttt tattactgca ttccagctag tgatcaactg
    11881 ttggttcatt tcatccacat ttgcaaagag ccgcggagca aagtcgtttt ttcctaataa
    11941 gcgttcatgg aaaaagtgca tatcactgat aaagtaattc atgtatcttt tgcctccctt
    12001 ttttcttaga gtaaacaaaa tcacttatct gagcaactca taacatctaa aaagaaaagg
    12061 ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat tttgaaaaat agtttttcac
    12121 aatttataaa attattcctt attgccgaaa ggctggccct ttttatgagt atgccaattt
    12181 tttatatcga atgctcaaaa gatgagtgat tttttggttg actatcatgt ttgtgtactg
    12241 aataggtcca gtagaacagt atttttacga cttgataatt aagggatgaa attttcagaa
    12301 atatgacgta taatgtaaaa tactgaaaag ttgcgacctt caactcgtag atgtttcaga
    12361 atctaattga aatgatggtg atatcatgac agacaacagc aaaacacgcg ttgtcgtagg
    12421 gatgagcggc ggtgtcgact catctgtaac agcacttttg ttaaaggaac aaggatacga
    12481 cgtgatcgga atattcatga aaaattggga tgacacggac gaaaacggtg tatgcacggc
    12541 cacggaagat tataaggatg tagcgaaagt agctgctcaa atcgggattc cttattactc
    12601 tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa tatttcctag ccgaataccg
    12661 cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa gaaatcaaat tcaaagcatt
    12721 cttggattat gcaatggatc ttggcgccga ttatgtagct actggacatt atgcgcaagt
    12781 cactcgcgat gaaaacggcg tggtccacat gcttcgtgga gtagacaata ataaagatca
    12841 aacttacttc cttagccaat tatcacaaga acagctttca aaaacgatgt tccctctagg
    12901 cggcatggaa aaatcagaag tacgggcaat tgctgaacgc gcaggccttg cgactgctaa
    12961 gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag aacttcaaac aatttttgag
    13021 caactatctg ccagcaaaaa aaggaaatat ggtcactctt gatggtgaag tcaaaggaca
    13081 acatgctggc ttgatgtatt atacgattgg tcaacgtcaa ggactaggga tcggtggcgg
    13141 aggagattcg caagaaccct ggtttgttgt aggaaaagat ctagcgacaa acacattata
    13201 tgtggggcaa gggttccatc atcctgcatt gtatgccaca agcttggatg caagtgaaat
    13261 ccactttacg acgaatgaac caatgccaaa agaattcaag tgtacagcga aattccgtta
    13321 tcgtcagcaa gacgttcctg tgacagttcg cttattggat gataatcgtg cagaagtcgt
    13381 attcgacgaa cctgtacgtg caatcactcc tggacaagca gttgtatttt atgatggaat
    13441 ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa gaaacaaaag ttttacaata
    13501 cgtttaagcg atattaaatg aatcaaaaca gctatggcta acaaaagaaa caccttttct
    13561 tttggatagc atagctgttt ttttgcaata aaattaattt ttattcattt tatacttgaa
    13621 atacttgtgt aaaaagttca taatatccaa taatatgaat aaagttgctt tttgttaagc
    13681 aagagcagtt gcaaaataga agggaatctt ttgcaagtga tttgttcaat tttataagtg
    13741 agggtgatgg aaatggaaaa gaaatcgacg ggtattagca aatggggact ggttgctctg
    13801 gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca caagtgatct ggctagttct
    13861 gcagctccag ggccagcgat cctttcatgg ataatcgttg ggattggtat tttagctttg
    13921 gtattatcgc tgaatcattt aggagaaaag cgtccagatc tagatggtgg tattttcggt
    13981 tacgcggaag cttcatttgg taaattaggt ggcttcatca gtggttgggg ttactggttg
    14041 tctgcgtggc tgggaaatgt tgcttttgca acgatgctga tgagcgccat gggagaattt
    14101 ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt tatttgcaag tatttttatt
    14161 tggggtctga ctttattagt aaacaatgga gttgaaggag cgagcgtcat caatacaatc
    14221 gtgacgatct gtaaattagt gccattattc ctatttttaa tatttgtatt tatcgcattt
    14281 aaagtgaata tattcactgc tgatttttgg ggtaacgtat ctgataatct agtgaatgga
    14341 acagggcagc aaggaacgat ctggttgcag attaaaggtt gtttgatggt tatgatgtgg
    14401 gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc gcgcgaagaa acgttcagaa
    14461 gcccagcaag catcgatcat tggtctgctt tgtttattgt ttatctatat ccttgcttcg
    14521 atcttgccat acggcgtctt atctcaagaa gagttagcaa ctatttcaca acctgctatg
    14581 gcaaatattt taaaaggaat cgtgggaaat tggggagcag cgctgatcaa tatcggcttg
    14641 attatttcga tcattggttc ttggttgtct tggacgatgc tcccagcaga aacaacgatg
    14701 ttgatggctc gagacggtct gttgccgaaa ggttggggaa aaacgaataa aaaaaatgcc
    14761 ccaacttatt cgttagttgt tactgcaatc ttgactaatc tattcttatt gactttctta
    14821 gttactgatt atgcctatca atttgcctat tctttatgta cagcggcaat tttgatttgt
    14881 tatctacttg tcgggatcta tcaagtccag ttttctttaa aagaaggaat caaaaaacaa
    14941 gtagtgatcg gtgggattgc tgtagttttt gaattgatgg gcatcatttt agcaggattt
    15001 tcttatgtcc ttttatgtag tattgcctat cttcctggtt tttgcttcta ctggatcgct
    15061 tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag taatgattgg gctgatttgt
    15121 gtggccgcac tgctttcaat cgggttgctt gctgtaggct ggatcaagat ataaggggga
    15181 aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg ctaaataaat gggaaaagaa
    15241 agcggtatat gatattgcac aaagctcgat cgaagcattg acattagacg aactcgttgg
    15301 cttagatggg actagcgtaa gtaaattttt cgaaaaacgc aaaaacgaac cattggacta
    15361 tggctggatc gaaggatcag atgttttcaa acaagaagtc gcttctcttt atcaaaccgt
    15421 cgatccggaa aacatcttac aaacgaatgg agcgactggt gctaatcttt tagctctata
    15481 cgcgttagtg gaagcaggag accatgttgt ttcgatgctt cctacttatc agcaattata
    15541 tgacatcccc aaatctttag gtgccgctgt tgactttgtt catttgaagg aagaagaaga
    15601 ttggcagttt gatttagaac agctagaagc cttagtcaaa cctgaaacaa aaatcatctg
    15661 tttgaatagt gcaaacaatc ctacaggcac attgctagat cggaaaactt tagaaaaaat
    15721 cgcagaaatt gctcgtacag tagatgcata tgtattgata gatgaagtat atgcgccttt
    15781 aacggataaa ggtgaattct tgtcgatcgt agatgtctat gaaaaaggta tagcaaccaa
    15841 ttccctatcc aaaacctact ccattccagg tattcgtatt ggctggacag ctactggacc
    15901 tgagttggca gaagtttttc gaaagtatcg agactacacg atgatttgcg gcggagtcct
    15961 gtcagatgat cttgctgtac atgcattgaa gaataaagaa aaaatattgg cgcgaaacaa
    16021 aaagatcatt acagagaacc ttgtgatttt aaaacaatgg gtagccaatg aaccgaaagt
    16081 tgaacttgtc gctcccaatt acgtctctac ttctttcatt aaattagcca tcgaagaaga
    16141 tgaccagaca ttttgtatca atttattaga agaaacagga gtactattag taccaggaag
    16201 tgcctttgat ttaccgaagc atgcccgact aggttactgt tgtaaaaaag aaaccttgga
    16261 aaagggatta ttcctcttat ctgagttctt gaaaaaacag gcctaaacaa aaacactgca
    16321 aggacagtct gtgacaactg ttcaagcagt gtttttcatt tacgcttatt gtctatgaga
    16381 aggagagtgc cctgaagcta tggagactcc tgaagctttt tcttctgcag tttcttgctg
    16441 atggatctct tgtgtattgt gtcgtacaag caagaagttg accaaaacaa gaagagaagt
    16501 cgaaagaaag acgccgcgat aaccaaatgc actggaaaca ctggagccga tcataggccc
    16561 tactacgtta ccagttgctt ggaaagattg gttataactg aagatccgtc cagcagcttg
    16621 atgaggcgaa tattttgtga tcaatgcttg gacagcaggg agcagacaag catcagagat
    16681 accaatcaaa aaacgtaagg cagctaattg ccaaacattc gtgacgaatg ccattgggat
    16741 ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt ctttcactcc caatcttgtc
    16801 acctaagcga ccaaatcttg gagcggctat caaagtggca atcccaggta tcgaagcaat
    16861 gattccgctg atcaacgtaa cattcccatg tccatgcagc aattggcgaa tgtacagact
    16921 gatgattgga ctaatggaat tgttggatgc ttggatgatc atcgttgtga cgaacatacc
    16981 gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt ttggcagaaa ccatgttttc
    17041 ttttttcact gggacaaatt tttcatggac gaaaaacaga ctcaacaaga agacgaaaaa
    17101 taagatgatc cctgtaatga aaaaggtagg gcgatagcca aacgctgaag cagagatccc
    17161 gccaagaagc ggtccaagta atgttcctgt aacagaacca gttgctaagg ttccaagaac
    17221 ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg gcagtggcat tactgatata
    17281 accagaaaaa atcccttgaa gcagacgcaa tgcgactaat tgatacacac tagtcacgca
    17341 acccatcaaa gaaatgacga ttgccattcc taatgaagca cgcaacagca tcaattttct
    17401 tcctttttgg tcggctaatt ttccccacca aggagagata agcgtagtca ccaagaaagt
    17461 agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac gtactataat tacctaacgt
    17521 atctatgtaa agtgacataa aaggcatgac taaactaaat ccgataccag ccataaatgt
    17581 tccaaaccat aagacaaaca agttttgttc ccaagtttct cttttcctaa ataatttttc
    17641 ctttattctt gcagccaatt agactccttc tttctgaaaa ttttcatcta cagctcttta
    17701 ttataaacta caatatgtag tttatcaatg aaagtctctt ttttctcttt ctttttcgtg
    17761 gtaaactgaa agagtgaagg atttatttta tgagaggaaa catggaaatg aaaaaaagaa
    17821 atctgtctca agagaaaatc atcgactgct ttcgagaact agcagaagag atggatgttc
    17881 agcaaataac ctttcagcat ttagccaaac gcttggatat caaggctccc tcgctgtata
    17941 accatttcaa aaatattcgt gatgtcaaaa cagcgctaac agcgaaactt ctgcaggaac
    18001 ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg tcaggaagca ctgcagatct
    18061 atgcagaaac ttatcgatcc tttgcttttt caaatcaagc agtgtatgag ttactgatca
    18121 gtgtaccgca taccaatgaa gctgttttat tagatggaat ccatgaaacg aatcagatca
    18181 ttttgcagat atttgaggct tttcaattaa cgaaagaaga aaaagttcat cgaagcaggg
    18241 aacttcgtag tatgatccac ggttatcttt ccttacgttt tttaggatac tttacaaaag
    18301 aaccaacaat cgatccagaa gacagctatt tttggatgat caataacttt atcgctactt
    18361 tgccttaaac aatcagattc atttgtaaca attttaagat gttcattaaa tgaccctttt
    18421 ataagagaaa attcttgcaa ctttcttgaa aagtgactaa gatgagtcta gaaaagctga
    18481 aagaaggagc acgagtatgt atcaagtaat taccatgttc ggggacaatg agccttggtg
    18541 gttttttgaa gactgggaag aagatattga agaagaagaa acattcgagt ctttcgaaga
    18601 agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat caaaattatg aatatatcaa
    18661 tgcaaaaagt aactttctca gcgccttttg gaatgacgga gatgagcgct ggtgtgaaga
    18721 gtgtgacgat gatttacagc aatacaaggg acttgcgttg ttgaaaaatc atcagcccat
    18781 cacagtagaa agcagaaagg aattttatga aacaactaat tctagcggaa aaaccaagcg
    18841 ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa gcacaaaaat tattatgaag
    18901 gacccaaggt gatcgttact tgggcccttg gtcatttatt aggattgaaa atgccggaag
    18961 atctgaataa ggaatggcag agctggcaaa tggacacgtt gccaatgatc cctaaaaaac
    19021 taggaatcaa accacttcct aaaacaggac atcaattaaa agcaatcaag cagctggctc
    19081 agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc cggaagagaa ggggaacttg
    19141 ttgctcgctg gatcttagag tatgtgcatt tcaataaacc agtgaagcgg ttatggatct
    19201 cttctcaaac agataaagca atcaaaactg gattcaagca actaagacct gcgaaagaat
    19261 atgatgcatt gtatgaatct gctcttgccc gggcaaaagc ggactggctg gtaggattga
    19321 atgtgaccag agccttaact gtgaagtacg cggataattt atcagcaggc cgtgtgcaga
    19381 caccgacact agcattagtg agagaccaag aaaagaaaat cgagcagttc cgtccacaaa
    19441 cgtatttcac gatcacgttg caagctggaa aagaacaagc caaaatgctt caaaaaaatc
    19501 aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt tcaattgatc agtacaagca
    19561 aaggaactgt cacagaagtc caagaaaaat taaaaacgga atctgcgcca ttgccttatg
    19621 atttaacaga gatccaaaga gaagccaatc aacgctatgg ctattctgca aagaaaacat
    19681 taagtcttgt acaaagtttg tatgagaccc ataagatcgt tacttatccg cgtacagaca
    19741 gcaaatatct gacaaatgac atgaaaagta cgatgaaaga acgattgcaa gcagtcagcg
    19801 actttgctcc tgaggtcaag cagtatttga aaaacggagc catcgtccaa cagacaaaag
    19861 tattccaaga cagtaaagtg accgatcacc atgcactttt gccaactgaa aacagagcac
    19921 gttatgaaaa attaagtaat gaagagcgaa aaatctatca aatgattgtc tctcgttttt
    19981 taggactgtt tgctcaaccc cataaagtca gccaaacaaa agttacggtt gaatttggta
    20041 aagaacgatt catttttcgg caaaatcgtg tcgtccaagc aggatggaaa ggcgaaactg
    20101 aaactgagac ggaaacagtc aaatgggaaa aaggaatgag aatcaatccc gatttcgcca
    20161 tcaaaaaaga actatctgca ccgcctaaac cattgtcaga agcaagcttg cttggtcaaa
    20221 tggaaaaata cagtctggga accccagcta cgcgtgcaga gattatcgag aaactaatca
    20281 aatctgaact gatggaacgt acccctcaag gactgcaagt atctcctaaa ggtaagcaat
    20341 tattagtatt ggtaaatccc tctttagtaa caccagattt gacagaaagc tgggaaaaag
    20401 accttgaagc tattgctgca ggaaagaaac aagcaggaac cttcttgaat ggaatcgaaa
    20461 aagaaacaaa acgcttggtg aatgagatca aatcaagcaa acaagaatat caagatttct
    20521 cgattaccca aaagaaatgc ccagaatgtg gggccaatct gcgcgagaaa aacacacgtg
    20581 acggcaaaat ctatgtctgc accaattcag attgcaacta ccgcagaaga aaagatccaa
    20641 aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat ggaaatcatc gaaggtaaaa
    20701 acggtgctta tttccgttgt aaatacgatg gaacgacaga aaaattgctt gataaaaaag
    20761 agcagaagaa gaaaatgacc aaacatgaag aacgacgctt gctgaaaaaa tatgcacaag
    20821 cagaagaacc agaagaaagc ccattagcag cagcactaaa agcagcaatg aagaagtaat
    20881 aagatatgaa agaggttgtg atagaagtgg tcagcttcaa gaaataagaa ggaatttacg
    20941 aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgaagaagc tacttctgga
    21001 acaccattta ttcggaaaaa aggactgtga caagactttt gtcacagtcc tttttcattt
    21061 aaaaaaatgt ttacttcatt tgacgtatat tattggtgag gcttcaattc atttggcttc
    21121 actttttctg tttctcttat gatataaaca ggacgctgtt tcgtttctaa gaagattttc
    21181 ccaatatatt tcccgatgat tccaagacat aacaattgga ttccaccaat gaacaggaag
    21241 atcgagacca tggaaggcca tcctgatgta ggatcaccaa acaacaatgc ccgcaaaata
    21301 atgaacaaaa ttgcaatacc agaaccgaca caagacaaag cgccgataaa tgaagcaatc
    21361 gttaacggtg catccgagaa gttcacgata ccatcaatcg agtagttgaa taatttccag
    21421 aaggaccaag atgtttcgcc agccacacgt tcgcggtttt cataagaaat atattctgtt
    21481 ttaaatccga cccagctaaa taatcctttt gaaaatcggt tgtattctgt taattctagg
    21541 acggcatcca ccatttgacg agtcatcaag cggaaatcac gagcgccatc gaccatttct
    21601 gtatcgctga ttttattgat caactggtag aattttttcg caaagaaact gcgtatcgca
    21661 ggctcgcctt cacgagtcga acgccgggtg ccgacacaat cgatatcaga agtagtgatc
    21721 cggttgatca tctcaggaag cagttcgggc ggatcttgca gatcaacatc catcacggtc
    21781 actaaatctc ctgtagaagc ttgcaatcca gcgtacagag ccgcttcttt cccaaaattt
    21841 ctggaaaatg agacaaagcg gacataagga taagctgagg ctagcttctt gattgctgct
    21901 aacgtaccgt cttttgatcc gtcgtcaata aacaaatatt cgaactcatg ggcacctttg
    21961 aatttttcaa cctcatcaaa aaaaagagga acagtttttt cttcattaaa acatgggaca
    22021 acaattgata ctttcatcgt ttctatcctt tcaatcgtat gtttatatat ggtttacatt
    22081 caaaatttaa taaaccttaa ataacacctt taaaatttga ttttacgtaa aagaacaggt
    22141 attctttttg taaactaacg tatttagttt accatgtttc ctcggtttat caaactttta
    22201 agaacaattt ttattagttg gcaagtagtt ttggatagag aaaaaggagg aagttccagt
    22261 ctattgacta ggagcaaaca aaatgaaaat acacacacct atcggctttt tatggttaga
    22321 tgcagatgaa tccggattga ctgctgtatc ttttcagcca atttcagaaa gcgaatcagc
    22381 gaatcagcga attttaacgc gcacacaaga acagtttgaa gaatattttg cgggaaaaag
    22441 aaaagaattt gatgttcctt tatctataaa gagaggtacg ccttttcagc aaaatgtctg
    22501 gaaagctttg tcgaccattc cttatggaga aatccgaacg tataaagaag tagccgttgc
    22561 tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca aatcgaacca atccattgcc
    22621 aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga aaactgacag gatatatggg
    22681 aaacgcaata gatggggttg aaatcaaacg ccaattgttg ttgttagaag gatatctgca
    22741 aaattaaaaa cagttgtgat tcggacattg ttccatgcga gaatcacaac tgttttttca
    22801 tgttcattct aaactttgat cagccaaata aatagtaaag ccatcactgt ttggagaagc
    22861 ccgacagcta atccataaga taagaagcgt gtaccttctt tcatgaattt tgcaaagtct
    22921 aaacgcagac caattgccgc taaagcagtg atctcaaacc agctgctgat aaaatgtgcc
    22981 gattggtcaa taactacagg taaagaaaac aagctgttga ccaaacaaaa gatcacaaac
    23041 cctaatacat accaaggaag cggtaatttt cgttttttct gcgtatcagt tggcgtttca
    23101 aacagagctg gttgtttctt gtgcatgaat ttttcaaaca gatagacaac aacaacgagc
    23161 atcataatac gtagaatctt aaatagcatg gcgaattcga caacagaatt gtcaagtaag
    23221 cttgccccag cgactacttg accaacagat tggagtgtac cacccaaaag tgcacttttt
    23281 tcaatgagat ttccaccaaa caactgtaat ccaagaaaag ggagagtcaa catcatcaca
    23341 gtacccagta gattgactag tgtgatgatc tgtccttttt cttcctcttt cgcatggatg
    23401 gaaggggcga ttgaagcaat agcagaagaa ccacaaacag cgtttcctcc agccatcatc
    23461 aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca agtaagcacc gatgatagtg
    23521 actgacattt gcagtaacac aaaaacaatt ccttttacac caatttgagc aatcgtttga
    23581 gctgtgacag ttgcacctag taaaaaaaca gagaattcta atagtttgct ttcagcaaat
    23641 tttgtgccga ttgctagaac aggctgttta aaaaaagtat ttcctaataa aatacccaat
    23701 aaaatagcaa tagtcgctcc acctaactgt ggcagataaa tagcaagaag cctgctgaag
    23761 gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt cgcggaccgc tgaatccttt
    23821 acagatttta ttggatgtga caatgatttc atttgatcgg ttcctttcgt caataaatac
    23881 taaaagcata ctcgaacata gtagcactaa accagtattt tgacaaaaga acaagaggtc
    23941 gtgacaaaag tcttgtacga cctcttgttt cgaataaacg gtgttccaga agtagcttct
    24001 acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    24061 tttcttgaag ctgaccactt cttttacgac ctcttggtaa acggtgttca aaagcttgca
    24121 tctgcggtaa taagcccaac cagtacaaaa acatgaacag ctgtttttgt ttggccgttc
    24181 ttattacttg atgcagaaca gctttttcac aacctattga atcgattatt aagcgatttt
    24241 tttagaaaca aattttccat gtttgctatg ttctttaatt aagaaacgta cgtaatgagc
    24301 aagaacgtag aagacaacta cacttgcaaa cagacattcc gcaactttta agaaatccaa
    24361 tacgctacta tagataccta aactgttgaa gtaatgaaca gtagtaaata tagctgttgc
    24421 agagataaca agcgggaatg taaaggctgc atagcttgga taaaaacgaa gcttgaacaa
    24481 acgtggaagc atagacagca caacgaaata caatacttga gataaagcga acaatgctac
    24541 gacaagataa aaattaggtt cagcaaagtt tttaagataa cctgttaggc aaagagaacc
    24601 aggtgctgca acgatcgtaa tcagcggacg tgtcggatga gccatatttc ttacgaaaaa
    24661 gacacgtttg atgatgatcg gcaataagaa cgcatagcag atcaatgaaa tccaaaagaa
    24721 aatttttcca ataagtggga agaagttccc ggcagttaca gtgatgaccc caaatccaac
    24781 gtaaatgata aaccagcttg gataaattgc ttcaattgaa agatcagctt tgataacatg
    24841 gtgataattg aaataaaaga caaggatcac ttggaagata acagcgcaca gccaaatgaa
    24901 tttgacaatt ggtgcaatac tttcatatga aacaaaataa gtacaaataa ccatgagtga
    24961 catcgtaaaa gttggtgaaa cggaggcgat gattggatca tgcaacgttt gtttcgtgtg
    25021 ttcgaataag ataaacaatt taacaaaaat gaatatcata agaatcatag agataccgcc
    25081 agttagattg cctaaagcaa catggtgata gtctttaaac agatttccaa gggaagctaa
    25141 cccaaggatc aacccgcaga tcgggatcgg aataattttc aaaaagttct tcatatataa
    25201 tattctcctt tttatttaat agattttagt accaagatga acgaatacat tgtaaaatga
    25261 ataggaatgt atgattacga gcaagcagac agctgattgt cttcatctaa gttactagtt
    25321 ggatcagcaa ctgttttgtt gtacatttct attatagatg gttttttgta tatttaaatc
    25381 aaatagtttt tatagtaatt atcataaaaa gttatggaag tggtgccatg tttaagttat
    25441 taacgacttt tcgagcagta tatgaaacaa gaaatttttc cagagcagca gaagtactgt
    25501 ttttatctca accagcagtc tcgaatcaaa tcaaacagtt ggagcgggaa ttgaatatcg
    25561 atctttttca acgtaacgga agaaaagaga tgcttccaac taagcaagcc gacttattat
    25621 atgaacggac actggttttg ttagaagaat gggaagaaac tcagcaaaaa ttgctcaatg
    25681 aacaaaatga agaagaagta tgtcggatcg ttgcttccca cacctttgct gtctatcttt
    25741 tgcctcagtt gatgaaacat ctgatcaaaa ggtttcctaa agtgcatttt gagatcgaaa
    25801 tcacgaattc tcatgaatca ttagaacaag tagcgaaaca tgaatccgat tttggtttta
    25861 tcgaaaagcc attagagact tccggcgtac aaagatactc tttgatggaa gaccagctag
    25921 tcatcgctgg ggacccaaaa agcaaattgt ggcttgtaag agaagcaaac tctggtgttt
    25981 ttcattacac gcagcgttat tttgaagaga tgaatatcca agataagaag atgatcgtga
    26041 aaagtaacga tgtaattgtt gcgttgctga aggaaggaat cggtcgctcg attttgtcta
    26101 agcgctctgt tccagaaaat gtgccaatga ctccgttgaa tttccatcga tatttttatt
    26161 tcatccaacg aaccaatctg gtttctgatc agctgctaga gattgccgaa gagatccttc
    26221 gatacaatga aagcacagaa acttctcaca gcgaggagaa tctcagctgg tagataattg
    26281 catgtatcaa aaaaagttct atgatacact aaagataagc agtaggcttt tgtactgcat
    26341 ttgctcaagc aactgcacaa acggaaagtt aatttttccc aaatgtctat ggaaagactt
    26401 tccattatct aactggaccg ctataataat cagcagtatg gtttttaaga aaagggatga
    26461 aacatggaaa aaaaattaaa agcttcgaac gataatcgta ttgattatgg cgtgatttta
    26521 ccagtctttc tgttatgttt gattggtctg ctctcattat atgtagcact tagccatgat
    26581 ccgaacaatc cagcagtctt gaaaggcgta gggatgcaag ttgtctggta tttagttgga
    26641 gcggcagcaa tcgttgtcat catgcatttg aattccaaat ggatctggaa actgactcct
    26701 tacctttatg gtttggggtt agtggtaatg ggactcttgc tcaaattcta tgatcagaat
    26761 ttagctgctt caacagggtc taaaaactgg tttcgattcg gtgcgtttac tttccagcca
    26821 gcagagttga tgaagattgc gtacatcttg atgatggcac tgatcgtaac taaacataat
    26881 acgcaagtaa aagaacggac gatgaaatcg gacttttggc tgattgggaa gctcttgatc
    26941 gtgacaatac cggttttagc attgatcatg gcccaagatg actttggaac gatgcttgtc
    27001 tttcttgcga tctttggcgg gattttcctg atgtctggaa tctcttggcg catcatcgca
    27061 cctgtagtaa tcttggcagt agtcgttgga gcaggaacga tcttcttggt tacgactgaa
    27121 ggcggaagag acttgcttta taaagttgga ttcaaatctt atcagtttgc acgtattgat
    27181 tcatggctgg accctttcca tgatacgtca ggaatgagtt atcagccggc ccaaggactt
    27241 ttggcaattg gtaccggtgg cttgtttggt aaaggattca acgtaagcaa tatctatgtg
    27301 cctgttcgtg agtccgatat gatttttacc gttatcggtg aaaacttcgg ctttatcggt
    27361 ggtgcattcg tcattttctt gtattttatt ttaatctatc gtatgattcg ggtgtgtttc
    27421 gatacgaata atgaatttta cgcctatatc gcaagcggtt tgatcatgat gctgctgttc
    27481 catgtctttg agaatattgg agcaaatatc ggattacttc ctttaacagg gatcccattg
    27541 cctttcatta gtcagggagg atcctcgatt ttagggaaca tgatcggtat cgggttgata
    27601 ttatcgatga gataccaaaa tgaagcacct gcaagatctt caaggaggta agaaaattgt
    27661 acacctttta ctggtacccg aaatgttcaa catgcaaaaa agccaaagcg tggttagacc
    27721 agcatggcgt tagttatcaa accgttgata tgatcaaaga agtaccgaag gcctcaacga
    27781 ttgagacttg gctagaaacg agtcaagtac caatccgtcg cctgttcaac acaagcggga
    27841 tgaaatatcg ggaacttgga ctgaaagatc aagtaggcga gtttacggct tcaaaagcga
    27901 gccaagtctt atcgacagat ggtatgctga tcaagcgacc actgatctta cgagaggacc
    27961 agcttgttgc aattggattc aatgaaaaaa tttatgaagg agaactgata taaaatggca
    28021 ggcaagcatt tgaagaaaaa ggacaacctt tggatcttat ttaatggaaa agaatactgt
    28081 gttggtttga caaatgaagc gcaagaagaa cttggagaca tcacgtttgc caatctgcca
    28141 aaagcaggac aatcgctaaa aaaagatgaa cctctgatcg aagttgaagc ggaaaaagcg
    28201 gtcaatgaat ttgccagccc gctaacgggt acagtttctt ctgttaatga taaaatcggt
    28261 gaagatatca atatcttgaa tgattcagat gaaatgaatg cgtggatctt aagtttcaaa
    28321 gatgttgatc caaaagaatt tgacgaatta taaaatcaga aaatcacttg aaaaaagttg
    28381 acactgattc ttccagttgc tataatcata agcaatccta tatgaaacga gaaagctttg
    28441 aaaagaagag tacatgtcac ggacgtttga agagagccct gtttgctgaa aatgggtaac
    28501 gaaaatgaga tggaagatgg tcttagagca gagtaagcga gcgtaagcaa acttactacg
    28561 ggagtgcccg ttacagcgcg acagtatacg gaaagaagct aagtccaagt actggaagag
    28621 gctgttattg cgaagtaacg gtaaatcaag gtggtaacac ggaagttcaa gctttcgtcc
    28681 ttttgcttaa ggcaagaggg cgaaggcttt tttgtttgaa aaattataag agaaagatgt
    28741 gaatggcatg tcattaatcc aactaacgaa tgtcaaaaag acattcaatg ggaaaaacgg
    28801 aaacatccaa gccgtcaatg acgtttcact aaacgttgaa aaaggggata tttatggaat
    28861 cgtcggctat tctggcgcag gaaaaagtac attagtccgt ctcctgaatg gacttgagct
    28921 tcccacgagc ggcgaagtga tcgtaaacga tcaagatatt acaaaattaa aaaacaaaga
    28981 attacgtaca ttccgaaaga aaatcggtat gatcttccag catttcaacc tgttatggtc
    29041 aagaactgta cttgaaaata tccagctgcc gctggaatta gctggcgtac caaagagcaa
    29101 acgtaaagaa cgggcagaag aactgttgcg tttagtcggg ttagaaggac gcggacaagc
    29161 atatccttca caactttccg gcggacagaa acaacgcgtc ggtatcgcac gagcattggc
    29221 aaatgatcct gagatcctgc tttgtgatga ggctactagc gcattggacc cgcaaacaac
    29281 agaagaagta ttagacttat tgttagcgat caataagaaa ttgaatttga ccatcgtctt
    29341 gatcacacat gaaatgaatg tgatccgcaa aatctgcaac aaagtcgctg tgatggagtt
    29401 ggggaaagtc gtagaagaag gagacgtatt gactgtcttc agacaaccga aaaaagaagt
    29461 aaccaaacgc tttgtacaac aagacatcga accagaagct gattcgacag aactattagc
    29521 tgaactcatc aaagaaaatc caagcggccg tttggcaact ttgaccttta gcgaaagaaa
    29581 tgccaacgag ccagtcattt cccaagcgat tcgacgctat aacatcgatg tcaacgttgt
    29641 gtatggaaag atcaaacaag tgaaagaagg ttcctttggt tcattgactg tcttgatgac
    29701 tggtgaaacc aaagaattag atcaagcaga agtgttcatc aaagaacaag gagtaggaat
    29761 tgaggtgatc catcgtggat aaaagctttt cagaaacata tctagatttt aaacaaatca
    29821 acccggaaac gatgaatcag gctatccttg atacactata tatgacatta atctcaatgg
    29881 tcttagtgac gatcatcgga ttagttttgg gattgatcct ttactctatt ggacgcaaaa
    29941 acaaaccgta tgctcgaatc atttatggaa tcgtatcgat catcagtaat attttccggt
    30001 caacgccatt tatgatcctg atggttttga tcattccatt tactaaagca atcgtaggca
    30061 ctatgctagg tgcgcaagca gccattccag ctcttgtttt atccgcagca ccattttatg
    30121 cccgtctagt agagattgct tttcgtgaag tcagccctgg tgtactggaa gcagcagatg
    30181 caatgggagc aagttactgg gaaatcatct ggaaggtctt gatacctgaa agtattcccg
    30241 cattgatttc tggattaaca gttacaacga tctcgatgat cggatttact gcgatggccg
    30301 gcgcgatcgg cgcaggtgga cttggaggtc ttgcttggca ggaaggctac cagcgtggta
    30361 atctaacagt gacattcgtt gcgactttaa ttattttact aatcgtattt atcgttcaag
    30421 gaatcggtga tttcttaact aagaaaacag acaaaagata ataggatata gggggatgta
    30481 ggatatgaaa aagaaaattt ttggatttgc agcagcgctt ttgctgactg taggattagc
    30541 agcatgcggt aacaacagcg acagcaaaga ttcagcaaat aaagcagacg acacgacatt
    30601 gaaagtcggc gcttcaccga caccgcacgc agagattttg gaacatgtga aaccattatt
    30661 gaaagatgaa ggaatcgatc ttgaaatcgt caaattcgat gactatgtat taccgaacca
    30721 atctttggaa gaaggcgaca ttgatgccaa ttacttccag catattcctt atttgaacaa
    30781 agaaatcaaa gaaaaaggat atgactttgt caatgccggt gctgtccaca tcgaaccaat
    30841 gggcttgtac tctaaaaaag tcaaagatgt ttccgaatta aaagatggtg caacaatcat
    30901 tacttcaaat tctgaatcag actggggacg catcatcaca attctacaag atgctggctt
    30961 agtcaaagtc aaagatggag tagatctgga aacagcaaca ttcgatgata ttgaagaaaa
    31021 ccctaagaac ttgaagttca accatacgat cgacccatca cttttagctt ctacgtacca
    31081 aaatgatgaa ggtgacctag ttgcaatcaa cgcaaacttc gcttacggcg cagggttgaa
    31141 tccattagaa gatgccgtat tgcttgaaaa ggacaactct ccttacgtga atattattgc
    31201 gactcgtaaa ggtgatgaag acagtgacaa aatcaaaaaa ctgattgaag tgcttcaccg
    31261 caaagaagtc caagaatgga tcacagacaa atggggcggc tctgttaaac cagtaaaggc
    31321 tgatgcaaaa taaggtatgg aacaaaaaag atgagaaata agtctgtaat ggcttatttc
    31381 tcatcttttt tatacctatt ctaatttaga aatcaaaatt gtttaataat aaataaaaga
    31441 tatttgattt tagtaaggaa ggtcattgcg cttcattctc aattagtcta aaatgagtga
    31501 gaacgaaaga gaatttgggg gtataatgca tgtctgtctt agaaataaaa aatctacatg
    31561 tgtcgatcga ggaaaaagaa atccttaaag gcgtaaacct aacaatgaaa accggagaaa
    31621 tccatgccat catgggacct aacggcacag gaaaatctac cttatctgcc gctatcatgg
    31681 gcaatccaaa ctatgaagta acagaaggcg aaattttgtt tgacgggaaa aatgttttag
    31741 acttggaagt agatgaacgt gcgcgacttg gcttattttt agctatgcaa tatccaagtg
    31801 agataccagg tatcacgaat gcggaattca tgcgtgcagc gatcaatgcg aaacgtgatg
    31861 aaaacgacaa aatttctgtt atggcatttt tgaaaaagtt agatgaaaaa atggctttac
    31921 taaatatgcc agaagaaatg gctgaacgtt atctgaacga aggtttttct ggcggagaaa
    31981 agaaacggaa tgagatcctg caattgttga tgttagaacc tacatttgca attttagacg
    32041 aaatcgattc aggtctggat atcgatgctt tgaaagtcgt tgctaaagga gtaaatgaaa
    32101 tgcgcggaga caatttcggt gcattgatca tcacccacta tcaacgatta ttgaactata
    32161 tcacacctga tgtcgttcac atcatgatgg aaggccgagt agtcatgaca gggaatgctg
    32221 acctagctaa acgtctggaa gcagaaggct acgctggtat cagtaaagaa ttaggaatcg
    32281 actataaaga agaagaagcg taagggggag caagaatgaa agaaaaaaac tggctcgatt
    32341 atcttgacgc ggtaaatgat ttttctttaa gtaaaggcga acctgattgg atgagaacct
    32401 tccgtcaaga cgcactagcg aaagcagatg aactgccatt gccgcatatc gatcgggtga
    32461 aattccatcg ctggtcgtta tttgatgtga aagaaacaca aaccatcagt gaaacaggca
    32521 cgattccggc atttgatgca atgaaagaca atcctgtgct tgtccaacaa ggttcatgga
    32581 cgatttttga acaactgcct gtggaattag cagaaaaagg agtcattttt accgacttgt
    32641 tcactgcaat gatcgaatat ccagaactag tccaagaata ttatatgaaa aaagcagtaa
    32701 acatgaacga agaccaattg actgcactcc atgtagcttt tatgaacagc ggtattttcc
    32761 tgtatgtgcc taaaaatgtc gtgatcgatg aaccactcga gtccttattc attcaagatg
    32821 gcgcatccga tgagcacttc ttcaagcatg tgttgatcgt ggcagatgaa catagtgaat
    32881 tttcttatct ggaacgtttc cagacaacca aagaacaagt agcaaaatca tcaggtaata
    32941 tcatcgtgga agtcatcgct aaagctggtt caaaaatcaa atattcagcc gtagatcaat
    33001 taggagaaaa tattacttct tatatgaatc gccgtgggca cattttacga gatgcttctg
    33061 tcgactgggc aattggtgtc atgaatgacg gtcatgtgat cgctgacttt gactcagatt
    33121 tagcaggaga aggtgcacac gcagaagtca aaatcgtggc aatcagctcg ggcagacaaa
    33181 tccaaggaat cgatacacga gtaaccaata aagcccctca tacgatcggg catattttgc
    33241 aacacggagt gatccgagaa aaaggaacat tgacctttaa tggtatcggc cacatcttga
    33301 aaggggcaaa aggtgcagat gcccaacaag aaagtcgtgt cttgatgctt tctgataaag
    33361 cacgtggtga tgcgaatcct attcttttga tcgatgaaaa cgaagtaact gctggacatg
    33421 ctgccagtgt aggacgagtt gatccagaag aaatgtacta tctgatgagt agaggattgc
    33481 ataaagaaga agcagagcgc ttagtgattc gaggattcct cggttctgtc ttaacagcaa
    33541 tccctgtaga acaagttcgg aaagaattag tcgaagtaat cgaagggaag ttgaatggat
    33601 gatcgatgct aaaaagctac gcaatgattt tcctatctta tttcaagacg tcaatgatga
    33661 acctttagtt tatctggata atgcagcgac gacccaaaag ccaaaacaag tactccaggc
    33721 attagcgtac tattacgaac acgataacgc caatgttcat cgcggtgtcc atacgttagc
    33781 agaacgcgcg acccatgatt atgaacaagc aagagaaaaa gtacgggcgt tcatccatgc
    33841 aaaagaaaca gcagaagtgc tgtttactcg tggtacgacg acaagcttga attgggtagc
    33901 cagaaccttc ggcgaccgct atatcaaagc aggagacgag atagtgctct cttacatgga
    33961 acatcattct aatgttatcc cgtggcagca gttggcaaaa cgaacaggtg ctgtcttgaa
    34021 atatatcgaa ctaacagaag aaggcttcct ggatatggag gatgcagcga aaaagattac
    34081 ggacaaaacc aaaatcgtct ccattgctca tgtatccaat gtcttaggtg tcttcaatcc
    34141 tgttcaagaa ttagctgaac ttgtccatga aaacggcggg gtcatcgtag tagatggtgc
    34201 tcaatctgcc ccgcacatgg ccattgatgt ccaagcacta gactgtgatt tctttgcttt
    34261 cagcggacac aaaatgtgcg gaccaacagg tattggtgtt ttatatggta aacgaaaatg
    34321 gttagaagaa atggaacctg ttgaatttgg tggcgaaatg atcgattttg tcgaattata
    34381 cgacagtaca tggaaagaac taccttggaa attcgaagca gggacaccaa atatcgccgg
    34441 cgctatcgca ttaggccatg caatcgatta tttgcaaaaa ctcggtatgg acaatatcca
    34501 tcgatatgaa gaagaactag ccgcttatgt gttgccaaaa ttacaagcaa ttgatggatt
    34561 gactgtttat ggtccacaag acccgaattt tcggacagga gtcgttgcat tcaatttgga
    34621 tgggttacat ccccatgatg tcgcgacagc tttagacatg gaaggggtag ccgtcagagc
    34681 aggtcatcat tgtgcgcaac cgctattgaa gtaccttgaa gtatcagcga ctgctcgcgc
    34741 gagcttttat ttctacaaca caacgcaaga cgccgaccgt ttagttgaag caatcatggc
    34801 aacaaaggag tttttccaac atgggactat ctaaattaga caatttatac cgtcaagtga
    34861 tcttggatca ttcttcccat ccgcatcatc gtggaagctt gactgactcg acaagcaaga
    34921 tcgaattgaa caaccccaca tgtggagatg tgatcgaatt gcaagtcgat gtaaaagacg
    34981 gaatcatcaa aaatatcgcc tttgacggca gtggctgctc catcagtacg gcaagtgcct
    35041 cgatgatgac agacgccgtc atcggaaaat caatagaaga agcagaacat ttaactgtag
    35101 actttctaca gcttgtccaa ggaaaagaag tcaaagatcc cgaccaatta ggcgatgctg
    35161 ctatgcttgg tggtgtagct aaatttcccg ctcggatcaa atgtgcgaca cttgcctgga
    35221 aagcactagg aaaagcaatg gaaaacgatg gtaccgcagc tttccatcat taccacaagg
    35281 aagaggagtg agaacatggg cgtaccagaa ttagaagaat ataaattcgg gtttcacgat
    35341 gatgtcgaac cggttttcag tacaggagaa ggcttgacag aagaagtgat ccgtgagatg
    35401 tcaagaatca aaggcgaacc agaatggatg cttgaattcc gtctgaaatc tctagaaaca
    35461 tttaacaaaa tgccaatgca aacatggggc cccgaccttt cagacatcga tttcgatgcc
    35521 atcaaatatt accaaaaacc aagtgacaaa cccgctcgtg actgggaaga cgtaccagaa
    35581 aaaatcaaag atacatttga aaagattggt ataccagaag ctgaacgagc ttatctagcc
    35641 ggtgcttccg cacaatatga atcagaagtc gtgtaccata atatgaaaga tgaattcgaa
    35701 aagttaggaa tcgtctttac tgacactgac tctgctttga aagaataccc tgaattattc
    35761 aaagagtact tttcaaaact tgtaccgcca accgacaaca aattagcagc gctgaattca
    35821 gccgtatggt caggtgggac ctttatttac gtgccaaaag gcgtcaaagt cgatgttcca
    35881 ttacaaacgt acttccggat caatgcggaa aacacggggc aattcgaacg tacgctgatc
    35941 attgttgacg aaggagcaag catccactat gtcgaaggtt gtacggcacc tacttattca
    36001 agtaacagcc tgcatgccgc catcgtagaa atctttacaa gaaaagatgc gtattgccgt
    36061 tacacaacga tccaaaactg gtcagacaat gtgtacaact tagtaaccaa acgagcaaaa
    36121 gcatacgaag gcgcaaccgt tgaatggatc gacggaaacc taggtgcaaa aacaacgatg
    36181 aaatacccaa gtgtttactt agatggaaaa ggtgcacgag gcaccatgct ctccatcgcc
    36241 tttgcaggag caaaccaaat ccaagatacg ggtgcaaaaa tgatccacaa tgcaccaaat
    36301 acatctagct ctatcgtctc taaatcaatt tcaaaagacg gcggagaagt aaactatcga
    36361 ggacaagtaa cctttgccaa aaacagtgcg ggttcgatct ctcatatcga atgtgacacg
    36421 atcatcatgg acgaatggtc gaaatccgat acgatcccat tcaatgagat ccacaacagc
    36481 caagtctctt tagaacacga agccaaagtc tcaaaaatct cagaagaaca actctactat
    36541 ctgatgagcc gaggcctgac cgaatctgaa gcaactgaaa tgatcgtcat gggctttgtt
    36601 gaaccattca ccaaagaact tccaatggaa tatgcagtcg aactcaatcg attgatcagt
    36661 tatgagatgg aaggcagtgt tgggtaaacc tgttatagga gctagaaccc ttgatatata
    36721 agggttctag cttttttgtt ttgcctgtat actagaaatg accagatttt gaccagctct
    36781 tttactgatg agccatcata tcagcaaagt cttgacctgt tttcttaggt gcattatttg
    36841 taatatgtgc atagatgtct aatgtcactt tactattttc atgccctaat cgttgttgaa
    36901 ctaccttgac agattctcct gcttctaata ataaactagc atgtgtatgt ctaaacccat
    36961 gaggtgtaat acgaggcaag ttatattttt tcaaaatata tcttaaccaa tcattaacag
    37021 tgtttggaat gtatagagtg tttttaactg ttgtaaaaac atgttgctct ttagagctag
    37081 tattgtatcc aagttttaaa tactcttctt tttgctgtaa tttccagttg ttgaggatag
    37141 aaagagtctc agtatctaaa gagataactc gtctagaagc tcttgttttc ggagattgaa
    37201 ttataacttt accaaattca tctacagcaa gagttttatt gacatgtagt tctttattaa
    37261 acgtgtctat gtctttccac tgtaaagcga gtacttcact ctttctcata cctgtgtatg
    37321 ccaataatct gaagaaggcg tattgtttca tgttaccaaa atctttaaaa gcatccagta
    37381 aattattaag ctcctctttt gagtaaaatt tacgattttt ctcatactct ttttttcgtg
    37441 gaagaagtgt tttttccata ggattattgt caatatattg cattgcaacc ccgtagcgta
    37501 aaatttgtgc agtctgtttt ctcatataat cgtaactttc atatttactg tgccattcat
    37561 ctaccacttt ttgacaatat gggacagaga tattcgatag tttaagctta ccaaatgctg
    37621 gaagtatttg attatttgcg tatcttactg agattgcgat tgttgaagct ttaacactgg
    37681 tacgctgatg agttatccat tcattataca ggtcttcaaa tgtcatatca ggtgctttct
    37741 ttttaacacc gtataatatt tgatgtctgt actcgtcata atctttttga gcttcttgtt
    37801 tgctactaaa acctcttctc gtagtattta ttttttcacc agttcgtgga tcggtggcaa
    37861 tgtgtccttt atacatccac gccacttgac cgtttttctt tttgtacttt gatatttttg
    37921 ccatgaaatc atctcctctt agtttacaag gagtacttac cactagatgg tatcacctcc
    37981 cttcattttt ttcttagtgt attttttccc aattgatgaa atcgtttgtt ctagcttgtt
    38041 tatattatca gtaatttctc tcacagcttc ttcgggataa gacatgtctt tcaattgaat
    38101 aggtagccat tcatctacca ttctttctag tagttcttct atactgctag tgaggaaaag
    38161 aatgttattg tcaaattctt ctacaatagc attgatgaat aattcttcta tttttaagat
    38221 attgaaaaga ctaatttttt catttttaac tttattacaa gtcttgttag caataaattc
    38281 ttgcatctct ggttctaatt tggaatatct atccagccat gtatctaaat tagagtgtac
    38341 tggtgggaac tgttgacgaa gatgctggaa caggttttta tctggaaatg ttataccatc
    38401 tagtacaatg ctatctctat aaagaataat tcccaaaata tattcttcta cattgccaca
    38461 aactaactcc tctatactca tatttcccaa atcagcaatt cgggataatc gctctggtat
    38521 tggtaaatag gttcccttct cccattcgta gatagttttt ttgtcaatta attgaccgtt
    38581 gttaatcata agaccaaatg ctggttgtgt taatttttta ctctttctta tagcttttat
    38641 ttttgttccg agtatctttt ttcgttctaa ataatttatt ggtggacgtt ttatcatatt
    38701 gaccacacct cctaaaatta gtatacctcg aaaaaggggt gttgtaaata aattttacaa
    38761 agatattgac acctcttttt tatcgaggta ttatgtgtat gtcgatgaac tggtaatcat
    38821 tttttgtttg attaatcggt taataacaaa atacttttat tttgagcaaa aaatacgaag
    38881 ataattctta gaaaatttgt tcaagcaatc acgaaaggag ggggaaatat gtctctacaa
    38941 atcattgata gcattgtttc tatacaagtt gaacaacaag tgaaagagat aattacagtg
    39001 ctaaagccta tgctagaaga cattatttct ggagtcactc tagagtatat ggactatcag
    39061 caggcaagtg aatatctagg tgtatcagta ggaacgatta gaaaatatgt ttctcaatac
    39121 gggttacctg ttataaaaat tgatacggtc attagattta aaaaatctga tattgatagt
    39181 ttcatggaac ggtataaagg ttgagtaaac aaataagact aaaacatata gtaataaaga
    39241 gtttgttttt attatctgta tgttttagtc tttaacagaa ttttataagg aggaaacaca
    39301 aatggaaaca tcaatgatta atattccaat ttatgtagat aaagaaaaat tattagagaa
    39361 gccagttaca aaaaaagaaa aaactgatgt aagagatagg tgtttagata actatagaga
    39421 agttacaaga gatgagtttg tagagatact aaattcatca caatcattta ttccaagcaa
    39481 gacaaagaat aaaggcaatg acaaggagag ttttgtggaa accagagtaa ttattctgga
    39541 tgtcgataac actgttaaag atgagaaggg gaaggtagta gatttgagta aagatgatag
    39601 tagatactta agcatagaaa aggttttatc tatcaattta gttcataatt ctgcgtttgc
    39661 gatacaaaaa agtattcggt actctgaaaa tctagaaaaa tacaagattg tctttctatt
    39721 aaaagaagca attactgact atggtaaaat ggttgctgtt tacgaatact tgaaaaagaa
    39781 catccccggg tgtgatgaca atgtaaatgc ttcgaataga atgttttttg gaggatataa
    39841 gtcagatgca gtgataataa ttaatgaaga caacatgtta gatattactg agcttccgat
    39901 tgattttacc atgacatgtt ctaaaaattg tcaaagtgat tcagggagag agtatgaact
    39961 agtcaatgaa accgattttg ttaagttgat aaaaaatgac gacaaagaag aaatgaaaca
    40021 gtggtttaaa gactgcctgt ttgattcctc cgacatgtct tttaatgaga tttatgaaag
    40081 attgttaacc attgatatga ataaattact taagtcaagt aaaaacttaa gatgtctgtt
    40141 tcacaacgat cataatccct ctgctagtat ttttaccagt aaaactgggc attccatttt
    40201 ttattgtcat tccagtaatt gtgatatcag tgaaaatttt attggagttg tgatgctaat
    40261 tttaaagaaa gacagtcggg tagaaacatt taattggcta ttagagaacc tagacttgta
    40321 tcctacacat tttcgaaaat tgaaagagga atcaaaagta ctttttgaca ctcttgaatc
    40381 aatgaaagac aaacttttca ggacaataag gaattatcta gaggaaatgc gacgtgtcta
    40441 tgatgtcttg ctttctgatg ttaatttttt tgatgatagt aaggaaagtg tgacttgtat
    40501 tttgtcgggg gaacaattag ctaaaaaaat gagtttttac tatggtaaaa cttatgacat
    40561 agataaatgt aataagttac tatcacttat gacttttata ggattgataa aaaaattaga
    40621 tgatgaagat gttcctttga agatgctcga atacctaaat aggttaaagg aagagaaagc
    40681 gttaggaaac tttaatcata agagaagcaa tgtctatcga ttagacgttt ttaatgcaac
    40741 ttctgtgatt caaaaattga atgaagatat ccttccactt ctggaaaaat ttcattttac
    40801 atatcaatat ttttcttatg aatgggttaa gatatgtttt aacgaaaaag aggctaaaag
    40861 agtatttcct caaaattccc gatcagaact tagcaaggaa aaacaattga ttttagatga
    40921 agcacatgaa gttttacagg gcttatcgtg gcaaggaaca tatctaatga cagagaaaga
    40981 acttactaac actgtttgtg aacgtctacg taatgttggg aataacagcg tggaaaatgc
    41041 tttaaaagaa aacagaggct actttgttaa acaaggattc actcttgaac gagcttctaa
    41101 ggatattaag agactataca atgtgaaagc aagtagtagt tttttagtct acctagcaaa
    41161 aggagcaggg atagatgaag taataaaaga attgatatta tcaggagttg aattagttcc
    41221 tctttatcaa tttacaagtg taacaagaga tgatggacgg agagtaagga agagaaaaga
    41281 cccaattgta ttaaactaga caggttatcc atagctctta atcttatttc atctcaatac
    41341 acccaccgtt aaagttcggt gggttattta tttttcaatc tttcaatagc tgattgcgtt
    41401 cattttcttg cctttttaat agcttcatat agtcaattgt tcgaactgca tcttcagtgc
    41461 taagagaacg atagagagcc aaaaactctt cctcataatc tacgacaaca ttgtctagaa
    41521 aatggcttac aggggttcct agggctctag aaagagcggt taagctatcg aggttaggct
    41581 ctgttacaga attttcccat cttgctactg cctgttgtgt agagaagatt ttttcagcta
    41641 attctttttg tgtccacccg agagcttttc gcttttctct aatgaccttt gcatattgtt
    41701 tctgtttcat taatacaccc ccatctcagt atagtacaac aagttgttgt atttgtaatg
    41761 gtatttgaag tttttttata ttttatgagt tgaaatacat catattgttg tatataataa
    41821 aacaggaggg gagaatcatg aaaaaagtaa gtaatctcag ccgatacaga aaagaaaaag
    41881 gactttctca aacggagtta gcaaaaaaat gaatgttaca cagcaatgtg taagttcgtg
    41941 gcaaacaggg agaacgattc ctaaacccta tcaaatgaag atgctttctg agatattaag
    42001 tgtaccaatg aatgagctgt tttctgatgt atttaataaa gtaaacagtt aactaataaa
    42061 caattgaatg tttttaggag gagaagacat gtattgtaga aaatgtggca aagaaataac
    42121 agatgaaagt aagtattgcc agttttgcgg agcactacaa gaaacagatg agacaactgg
    42181 agcagacgca gtaaagaaaa gtactgagca gacaattaat gaaatgaagg aacaaatcgt
    42241 acatggacaa gagacaggga agctgtattt gattattggt tggatttcta tggtagtagc
    42301 gttagcattt attcctgttg tatttggtgc tgttgctgtt attatgggct acttatatcg
    42361 agataaagat gaacaaatgg gtacgatttt gatgatagca ggggtagctg gagcaatctt
    42421 tggtgtactt ttagggatgt caatgtactg ataagaggga taagagcaaa ttattttgga
    42481 gataggagat aaaaagttga ataaagtaaa tatgaacaag aagtttatca ttatagttat
    42541 cgttgtcttg gctattatag gtatcatttt ttcaaaaatg acaggtacat ctggattaga
    42601 gaagtcatcg aaaccattgg taacacagat tttacaagaa cagtacaact taaatcgatc
    42661 atgtgatgat gtgacgatta ctcatgaaaa tggagataac acataccggg ctaaagctat
    42721 attagacaat ggaagtgcta tcaacataaa tattgagtac tatccaaaga aagaccgtat
    42781 atatgtggag ataccatatg ctgaggtgtt aatgttaaat tgatttaaat gtattatgca
    42841 taccgtccaa acaggaactc ccaaaggtgt aaattcacta gggagttttt atagtttcct
    42901 ataaattaaa tactaataca ggtgacaaaa aaatattaca atgattgtcg ggaattttta
    42961 tgttattcac tgttataaca atataaaaga aatgaaaaag aaaaaattat tatggatatg
    43021 tttggtattt ttattgataa tt
//
LOCUS       DAZUMI010000009        42770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000009 DAZUMI010000000
VERSION     DAZUMI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.009"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG81C_000381"
     CDS             complement(132..509)
                     /locus_tag="ACG81C_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063573.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG81C_000382"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG81C_000382"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5063574.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG81C_000383"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG81C_000383"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5063575.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG81C_000384"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG81C_000384"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5063576.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG81C_000385"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG81C_000385"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5063577.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG81C_000386"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG81C_000386"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5063578.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG81C_000387"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG81C_000387"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5063579.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG81C_000388"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG81C_000388"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5063580.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG81C_000389"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG81C_000389"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5063581.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG81C_000390"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG81C_000390"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5063582.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG81C_000391"
     CDS             10661..11575
                     /locus_tag="ACG81C_000391"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5063583.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG81C_000392"
     CDS             complement(11708..13273)
                     /locus_tag="ACG81C_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063584.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG81C_000393"
     CDS             complement(13266..14717)
                     /locus_tag="ACG81C_000393"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5063585.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG81C_000394"
     CDS             complement(14731..15747)
                     /locus_tag="ACG81C_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5063586.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG81C_000395"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG81C_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5063587.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG81C_000396"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG81C_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5063588.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG81C_000397"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG81C_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5063589.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG81C_000398"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG81C_000398"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5063590.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG81C_000399"
     CDS             21340..22764
                     /locus_tag="ACG81C_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5063591.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG81C_000400"
     CDS             complement(22806..23063)
                     /locus_tag="ACG81C_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063592.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG81C_000401"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG81C_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5063593.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG81C_000402"
     CDS             24337..25314
                     /locus_tag="ACG81C_000402"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5063594.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACG81C_000403"
     CDS             25339..26982
                     /locus_tag="ACG81C_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS5063595.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG81C_000404"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG81C_000404"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5063596.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACG81C_000405"
     CDS             28558..28833
                     /locus_tag="ACG81C_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5063597.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACG81C_000406"
     CDS             28839..29606
                     /locus_tag="ACG81C_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5063598.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACG81C_000407"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACG81C_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5063599.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33623
                     /gene="recQ"
                     /locus_tag="ACG81C_000408"
                     /pseudo
     CDS             31864..33623
                     /gene="recQ"
                     /locus_tag="ACG81C_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     gene            33737..34039
                     /locus_tag="ACG81C_000409"
     CDS             33737..34039
                     /locus_tag="ACG81C_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5063600.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34042..35205
                     /locus_tag="ACG81C_000410"
     CDS             34042..35205
                     /locus_tag="ACG81C_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5063601.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35231..38659
                     /locus_tag="ACG81C_000411"
     CDS             35231..38659
                     /locus_tag="ACG81C_000411"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5063602.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38680..39804
                     /locus_tag="ACG81C_000412"
     CDS             38680..39804
                     /locus_tag="ACG81C_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5063603.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39785..40231
                     /locus_tag="ACG81C_000413"
     CDS             39785..40231
                     /locus_tag="ACG81C_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5063604.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40215..40553
                     /locus_tag="ACG81C_000414"
     CDS             40215..40553
                     /locus_tag="ACG81C_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5063605.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40672..41232
                     /gene="rsmD"
                     /locus_tag="ACG81C_000415"
     CDS             40672..41232
                     /gene="rsmD"
                     /locus_tag="ACG81C_000415"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5063606.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41229..41720
                     /gene="coaD"
                     /locus_tag="ACG81C_000416"
     CDS             41229..41720
                     /gene="coaD"
                     /locus_tag="ACG81C_000416"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5063607.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41710..42753
                     /locus_tag="ACG81C_000417"
     CDS             41710..42753
                     /locus_tag="ACG81C_000417"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5063608.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        14115 a         7332 c         9551 g        11772 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt atccagcagt
    32881 cccaaagaga agaagggcaa aagcaaaaag aatatgaaaa attaaaagcg atgacagaat
    32941 acgtgtatat tgagtcctgt cttcagcaat acatcctaaa ctattttggc gagacaagca
    33001 gtccgtgcaa ccgttgtggt aactgtttag atgacagaga aatagtagaa gtaacgacag
    33061 aagcacagat ggttctttct tgcttaaaac gtatgggtga gaactatggt aaacaaatgc
    33121 tgatgaaagt cttagcagga tcgaaagaac aaaagctgca agctttaggt tttggacatt
    33181 tatctaccta tggactgatg aaaaaccaat cgcaaaaaga aacgatgcag ttaattgaat
    33241 atttgatatc gaatggctat cttctgacca tcaatggtga gtatcctgtt ttgaaagtaa
    33301 cagaaagagg gatacaagta ttgaaaggac aagaatctgt ttatcgaaag gaacccaaga
    33361 aagtccagca gctatcggat gaagaaaccg atacactatt tgaagtgctg cgggaactgc
    33421 gaacggatct agcatcagaa gcaggcgttc ccccgtatgt cgtattttca gattccacat
    33481 tgaaagaaat gagccgaata cgtccatcaa gcagattgga gatgcttcag ataaaaggag
    33541 ttggccaaag caagcttgat aaatatggcg aagccttttt atctcggata aaaaatgcgg
    33601 actctaacgt tctggataag tagaaagaac gaatacgtaa aaatgaataa aaacaactgg
    33661 ttaaatctgt tttttttgct ccttatgaaa atcaaatatg ttataattaa ggaattagaa
    33721 tgggaggtaa gcacagatgg attctttgtc gaaaaaactt gcgatccaag tattaaatga
    33781 tgaagcagaa cggatcaaac gattaatccg aaatcaaaac aacagtctct gtatctcaca
    33841 atgcaaagcc ttcgaagaag tggtagatac acagatgtac ggcttttccc ggcaagtcgg
    33901 ttttgctgtc cgtataggag taatcggaaa gaacgagggc cagctgatgt taagtgagct
    33961 agaacgagaa ttgaatcagt tatatagtga ggtttaccaa gaaatttatg ataaaaaaga
    34021 agttggcaag gaggaataga cttgcctaag aaagtgaaaa aaaggcatct attagactat
    34081 agcatcttga ttccctattt aattttatgt gtattgggat tgatcatggt atatagttct
    34141 acgtcgtatc tattattaga aaacggacaa aatccgtccg catcagtcat taaccagagt
    34201 attttttggg tgcttagctt gattgctatc gcactacttt ataaaatgaa aacagatgtt
    34261 ttgaaaaacc agcgtttgat catggcagcc atcgcagttt tgacgatttt gctgctgatt
    34321 gttgtgtttt ttggtaagga gatcaatggg gcgaaaggtt ggctgcaaat tgcaggattc
    34381 tcgatccaac ctgccgaata cctaaaaatc atttctatct ggtacctgtc tctgacttta
    34441 tctaaaagac aaaacagtgt acaaaaagat tttcttggga ctgtgaaacg tccacttgca
    34501 atggttatcg gacttactgc attagtcgct attcttcccg attttggtaa tgcggcagtt
    34561 atctttttga ttattttggt tctgctttta gccagcggag tcaattatgt gtatactttg
    34621 attgtaggag taggaggctt ttgtctaagt acttttacga tctggctgat caatatcaca
    34681 aacggaaaga tcttacccgg acgtttacag tatatctata atcgatttgc tatttaccaa
    34741 aatcctttct cagacgaatt aaataaaggg catcaactag tcaacggcta ctatgccatg
    34801 ttcaacggag gactatttgg caggggatta ggtaacagta tccagaaaaa aggattttta
    34861 caagaagcac aaaccgattt tatttatgca atcgtagtgg aagaactggg agtgattatg
    34921 gggattttga tattagcgtt gctattcttt atgattgtgc gcattgtcct tgtaggtatc
    34981 cgttcaaaag atccgttcaa ttcgcttttg tgcattggga tcggcgcaat gtttttgatc
    35041 caagtatttg tcaatcttgg aggaattact ggcgttattc ctttgacagg gatcacgttc
    35101 ccatttttaa gtcaaggagg ctccagtttg ctgatgcttt cgatttgtgt cggctttgtc
    35161 ctcaacatca gtgcggatga aaaacgcaag agcttaggtt tataataaaa aaagatgtag
    35221 gagtggttcg atgaaaaaag ttttagttgc taacaggggt gaaatcgctg tacgtgtatt
    35281 tcgtgcttgt acagaattag gaattaaaac tgttggaatc tatgcagaag aagatgaata
    35341 ttccgtacac cgtttcaaag cggatgaagc atatttagta ggacaaggaa aaaaaccgat
    35401 tgatgcgtat ctagatattg aagggatcat ttctatcgcg aaagaatgcg gagctgacgc
    35461 tattcatccg ggttacggtc ttttgtcgga aaacttgaat tttgcgcaac ggtgcgaaga
    35521 agaaggcatc atttttgttg gacccaaact gcatcattta gatatttttg gagataaaat
    35581 caaagcaaaa gctgcagcta tcgaagcggg gattgcttcg attccaggca cagatggacc
    35641 aatcgcttct atagacgatg ccttggagtt tgcgaaacaa tacggttatc cgattatgat
    35701 caaggcagct ctaggcggcg gcggacgcgg catgcgtgta gctcacgatg aaaagagtgc
    35761 aagagagggc tacgaacgtg cgaaaagcga agcaaaagca gcctttggta gtgatgaagt
    35821 gtatgttgaa aaatacatcg ctaatcctaa gcatatcgaa gtacagattc tcggggatac
    35881 gcatggaaac gtcatccatc tttttgaaag agattgttct gttcagcgcc gtcaccaaaa
    35941 agtagtagaa gtagcgcctt gtgtctctat gaatgaacag caacgtcaaa aaatctgcca
    36001 ggcagctgtc caattgatga aatatgtcgg gtatgtcaac gcaggtacag tagaatttct
    36061 tgttgaagga gatgattttt actttatcga agtcaaccct cgcgtccaag tggaacatac
    36121 gatcacagaa atgattacag atattgatat cgtaacaacg caactgttga ttgctcaagg
    36181 tttggatctg cataaagaaa tcggattgcc tcagcaagaa ggaattaaat taaatggttc
    36241 agccattcaa tgccgaatca ctacagaaga cccgttgaat aactttttac cagacacagg
    36301 gaaaatcgat acgtatcgtt cacctggtgg ttttggcgta cgtctggatg tcggaaatgc
    36361 ttacgcgggc tatgtagtga caccgtattt tgattcattg ctagtaaaag tatgtacgca
    36421 tggtgctacc tttgaaacag ccattcagaa aatggaacgt tgcttgaaag aatttagaat
    36481 ccgtggagtg aaaacaaaca ttccatttat gctcaatgtc atcacacatc ctgaattcca
    36541 atcaggaaat gcaaaaacaa ccttcattga tagtacagca acattatttg aatttcctcg
    36601 actacgagac cgcggaaaca aaacgatgaa atatatcggt gaaattactg tgaatggttt
    36661 tccaggtatc gaaagtggcg aaaaaccatt ttatgaagaa ccacgtatgc ctaaagatct
    36721 aatcacaaga actgattacg taacggctaa gaatgtatta gatgcgaagg gtgccgatgc
    36781 tttggtcgaa tggatcaaag gacaggaaaa tttgttgttg acggatacga catttagaga
    36841 cgcccatcaa agtctgctag caactcgtgt tcggacaaag gatttcaagc agattgcacg
    36901 tttgactgga gaaggtcttc ctgaactctt ttcaagtgag atgtgggggg gagcaacatt
    36961 tgatgtggct taccgctttt tgaatgaaga tccttggcaa cggttgagaa aaattcgttc
    37021 tttaatgcct aatacgttgc tgcaaatgct tttcagaggt tcaaatgccg ttgggtattc
    37081 taattatcct gacaatgtat tggtggaatt tgtcaaagaa gcagcagcgc aaggaatcga
    37141 tgtatttcga atctttgata gtttgaactg gactccacaa atggaaaaaa gtatccaagc
    37201 tgtacgggat actggtaaaa tcgcagaggc agctatttgc tatactggcg atatcaacga
    37261 tccaagtcgg gcaaaataca atgttcagta ttataaagac atggcaaagg aactagaaca
    37321 actgggtgcg catatcattg caatcaaaga tatggctggg ctgcttaaac cgcaggcagc
    37381 ttatcgtttg attagtgaat tgaaagaaac gacagatctg ccaatccatc tgcatactca
    37441 tgataccagc gggaatggaa tcatcactta ttctgctgct tcaaaagcag gagtagacat
    37501 cgttgacgtg gcaatgagtg caatgagtgg gaatactagt cagcctagca tgagcagctt
    37561 gtattatgca ctggtcaatg gtccacggct gccagaaatc acgattgaaa atgcacagaa
    37621 attaaaccat tattgggaag atgtgcgcat gtattacaaa ccgtttgaaa acggcttgaa
    37681 tgctcctgaa acagaagtat atatgcatga aatgccagga ggacagtatt cgaatctaca
    37741 gcaacaggca aaagctgtcg gattaggtca tcgctgggat gaaatcaaac aaatgtatca
    37801 tactgtcaac ttgatgtttg gcgatatcgt taaagtaact ccatcatcta aagtagttgg
    37861 agatatggca ctatttatgg tccaaaatga tcttacagaa gaagatattt atgaaaaagg
    37921 cgaaacacta agtttccctg aatctgttgt gactttcttc caaggagaac tagggcaacc
    37981 cgttggcgga tttcctaaaa aattgcaaaa gattattctt aaaggcagac ctgcattgaa
    38041 tgaacgtcca ggtcttttgg ctgaatcagt cgacttcaac gaagtaaaaa aagaattggc
    38101 agaaaaaatt ggctatgaac caaaacaaga cgaagtatta agctatttga tgtatccgca
    38161 agtctttttg gactatcaaa aagcatataa tcaatttggc gatgttacat tactggacac
    38221 accaacattt ttccaaggta tacgtttggg tgaaacaatc aatgtacaaa tcgaacgcgg
    38281 aaaaacgctt atcattcgtt tagatgaaat cggagaaccc gatatcgaag ggaatcgcgt
    38341 actgttcttc aatttgaatg gacaaagacg tgaaatcact atcaaagata attcgatcat
    38401 cagtacggtc caaacccgat tgaaagctga accgacaaac cgagaacaaa ttggagcaac
    38461 gatgtctggt tctgttttgg atgtattagt gaaaaaaggc gataacgtta aaaaaggtga
    38521 tacgttgatg attactgaag cgatgaaaat ggaaacggcc atcgaagcac gttttgacgg
    38581 agaagtcgca catgtatatg tgtcttcagg agacactatt tcgtctggtg accttttgat
    38641 tgaagtgact gagaaataat aagcgaaagg tggggaaaca tgaaaagact gggtttgttt
    38701 tggctgattc tgctattagt tattgtggca ttctacatgg aaccagtctt gtttccgcca
    38761 aaacttgaag tgaaggaaaa ctctgatcaa caaatatcaa accaaatgac aacgtggaaa
    38821 taccaggagc ttaaagcaac tggtttttct acttatatcg gaaaaccgat cgatcagctg
    38881 gaaaaggatt ttggccaagc ctatgatcga ttaactagcg gatttggttt tgaaacaaga
    38941 tattatgaaa ataaagctag tcatctcaca tttgaagcga acatccaaga cagcagagtc
    39001 acaactgtta aagtactgca aacagggcct gctgatatcg cgccgtttta tttagggatg
    39061 acgatgcaag atcttacgaa aatcactact atctatacta atttcacttt tgagtaccat
    39121 gatacagaag ttggaatcga actaatggaa gaggatatga attatcgtcc gctaattgct
    39181 tttgataacg acacttttgc tattttattt tttgatcaaa caacaaacgg cctattttca
    39241 gttgtgtatt tagataaaga tagtcttttg aaactattgc catatcaagt gtttggtgaa
    39301 ggccttccac attatcaagc agataaatcg gctgattggg aatcaatcaa tcgcgttaaa
    39361 gaacgaaaaa gtacgacttt attaaatatg cttagaagag aagacgaact tcccatatac
    39421 aatcaaacac ttgcctttca aaaccacagt cagctgctac tacatgattt tttgacaagg
    39481 cctgaggaaa tattgtcaga ggaacgtatg gctgattggc aaaaatcgct aggcagtgct
    39541 caaacgacag tgaaattctc actgacgaat gatgaattgg agaagttaat cagtaaacaa
    39601 aaattagaaa atactagtgg gctatttatt catccggcga ttgacgctac tttttcgttt
    39661 ttatattggt acagcgatcc ttacaaccat gaccgtttca tggacccggg aacggattct
    39721 gtaggcattg ctttttctaa agaaaatatg ttagtcttat tgcaagaaga aaacggagaa
    39781 agtagtgata gcagtgatcg ttaacgaaga gctgtttcaa ctggaggatc aatgcctcca
    39841 agtcgctgaa atgataaaaa agagtaaaac gatggcttct tatcttgaac ataaacagtc
    39901 aatggaagaa tctgcaaaag tttgccagtt gaaagaagcg ttcttgtcta agaaagaagc
    39961 atttgaaaat gtagcggcat atggtgagta tgcacctgat tatcgtgaaa aaaaacgagc
    40021 tgttcgaaaa gcaaaaagac agcttgatat ggatgagcat gttgcagctt tccgatatag
    40081 tgaaacacag ctgcaaagtc tgttggatca aattgtccaa gtacttgccg aagaagtttc
    40141 taatgaaatc aaagtagatg caggtagccc gttttttgaa acgaaaaaac actttggatg
    40201 cggaggtaat tgtcatgcaa ttagacaata gtgaatttga aataacaaaa cgacgcgggc
    40261 tgatcgtatg ggtctacagc ttaaaacaat taaagaatct gaaaaagttc ggcttgttgc
    40321 attatgtctc aagaagaatg aaatatgtcg tgctttactt aaatgaagag acattggaag
    40381 aaacagaaga acgaatccgt aaattacatt ttgttcgaaa cgtagaacgt tcttaccgac
    40441 cggatatcga aatgaatttt gccgaaaaaa taggaaacaa aacagtatta aaagacgaag
    40501 gtttcgaaat tgaagagcta aatacagaaa tccgattagc tgaacatctt taaaaatctc
    40561 ttatgagcag tttttaaata aaaaataact tggataacga tcgatttttg atcattgtcc
    40621 aagtattttt ttggtatact agtaccagta ccatttctaa ggagtgagta gatgcggata
    40681 atatcaggaa attatggcgg gcgccgttta aaaagtttag caggggcgaa caccagacca
    40741 actacagata aagtaaaaga atccatattc aatatgattg gaccatattt tgatggagag
    40801 acagtattgg atctgtttgc aggaagcggt ggtttagcta tcgaagcagt atctagaggc
    40861 tgcagtcatg cggtatgcgt agacaaaaat taccaagcgc taaaaatcat caaagaaaac
    40921 atcgaaatta cgaaagaacc agagaaattt accacaataa aacttgatgc ggacaaagca
    40981 atcgaacggc tagcagcgga gaaacaacaa ttcgactact tgtttcttga tccgccatat
    41041 gcaaaacaaa aaataatcga tcaaatccat aaaatggaag aactgggact tttttctaaa
    41101 accgcagtta tcgtatgcga aacggataaa acagtcgaat tgccagaaac tgtggctgga
    41161 ttccatcaaa taagaaaaca gatttatggc ataacggccg taactatcta tagaaaagag
    41221 ggaaatcaat gaaacgtgca ttattcccag gaagttttga tccttttaca aaaggtcact
    41281 tagatacggt ggaacgtgcg gctaaattat tcgatgaggt agtaattggt gtttttatca
    41341 atacttcaaa gaaaagtttg tttcccccag aagaaagaat gacattgatc acaaaagctg
    41401 tttctcatct tcctaatgta aaagtcatgc atcaagaaaa tcaattgacg gtagaaacag
    41461 caaaagagat tggtgcagat gcgttgatca gaggcatacg aagtatcaaa gactttgaat
    41521 atgaacgaga gattgctcag atgaatcatc atctatatga tgaattggaa actgtcttct
    41581 tattagcaaa accagaatat agtcacgtga gttccagtat tttaaaagaa gtactgcact
    41641 ttggcggaga tgtgagcagc tacttaccgc cagtgatcaa cgaagcttta gctggaaagc
    41701 gtgaatcaaa tgaaagctaa acaatggttg aaaacagtac tgattatcat atttggaatg
    41761 ttgatcattg gatttgtagt agttcccttg ccgtattatg tagaatcacc gggagccacg
    41821 atcgatctga aagaactaat tacggtcaat gataaggaag ataaaggttc tgggtctttc
    41881 tctcttacct ctgttggtat caggcaagct acaggtttct tagctttaaa agctaaaatg
    41941 accccttttg aagaattgat cagtgaagaa gaattgactg gtggggcgac aaatgaagaa
    42001 tataatcaaa tgcaacagta ttatatggat tcttcccaaa atgcagcaat cgaacaagcg
    42061 ttaaaacttg cagacaagcc ttataaaatg gaattcaaag gcgtatatgt catgagtgta
    42121 gagaaaacat cgaatttctt tggcaagctt tcagttggag atacagtaac aaaagtagac
    42181 gggaaaagct ttcaaagtac agaagcattt atggattatg taaaaagcca aaaagttgga
    42241 caaacaatcg aaatcagtta tttgcataat ggtgaagaaa aaacagcttc tggtgatctg
    42301 atcgagttac caacagataa aaaggcagga ataggtatca cgcttactga ccataccgag
    42361 atcgaatcag atacagatgt tcgtattaat tctggctcta tcggtgggcc atcagcggga
    42421 ttgatgttca ctttagaaat ctatgaacaa gtcactggta aaaacttgcg acatggtaag
    42481 caaattgcag gaactggaac gatcaacagt gaaggagaag ttggccgaat cggaggaatc
    42541 gataaaaaag tggcaagtgc ggataaagct ggcgtagaga ttttctttgc accagatgat
    42601 acgatttcag acgatgttaa gaaagaatat cctgaaataa aaaccaacta cgaagaagca
    42661 aaagctgccg ctaaaaaatt gaattcctca atgaaaatcg taccagtaaa aacagtacaa
    42721 gaagcgcttg actatctaga gaaaatgaaa tgaaactaaa taattaaaga
//
LOCUS       DAZUMI010000010        42384 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000010 DAZUMI010000000
VERSION     DAZUMI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.010"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..1151)
                     /locus_tag="ACG81C_000418"
     CDS             complement(48..1151)
                     /locus_tag="ACG81C_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5063609.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1373..1843)
                     /locus_tag="ACG81C_000419"
     CDS             complement(1373..1843)
                     /locus_tag="ACG81C_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5063610.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2176..3816
                     /locus_tag="ACG81C_000420"
     CDS             2176..3816
                     /locus_tag="ACG81C_000420"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5063611.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3970..5292
                     /locus_tag="ACG81C_000421"
     CDS             3970..5292
                     /locus_tag="ACG81C_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5063612.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5354..5785)
                     /locus_tag="ACG81C_000422"
     CDS             complement(5354..5785)
                     /locus_tag="ACG81C_000422"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063613.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5849..6685)
                     /locus_tag="ACG81C_000423"
     CDS             complement(5849..6685)
                     /locus_tag="ACG81C_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS5063614.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7427..8053)
                     /locus_tag="ACG81C_000424"
     CDS             complement(7427..8053)
                     /locus_tag="ACG81C_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063615.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8348..8683)
                     /locus_tag="ACG81C_000425"
     CDS             complement(8348..8683)
                     /locus_tag="ACG81C_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5063616.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(8670..8954)
                     /locus_tag="ACG81C_000426"
     CDS             complement(8670..8954)
                     /locus_tag="ACG81C_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5063617.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9010..10533)
                     /locus_tag="ACG81C_000427"
     CDS             complement(9010..10533)
                     /locus_tag="ACG81C_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5063618.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10526..11701)
                     /locus_tag="ACG81C_000428"
     CDS             complement(10526..11701)
                     /locus_tag="ACG81C_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5063619.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(11705..11890)
                     /locus_tag="ACG81C_000429"
     CDS             complement(11705..11890)
                     /locus_tag="ACG81C_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063620.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(11856..13550)
                     /locus_tag="ACG81C_000430"
     CDS             complement(11856..13550)
                     /locus_tag="ACG81C_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5063621.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13547..14020)
                     /locus_tag="ACG81C_000431"
     CDS             complement(13547..14020)
                     /locus_tag="ACG81C_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS5063622.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14089..14244)
                     /locus_tag="ACG81C_000432"
     CDS             complement(14089..14244)
                     /locus_tag="ACG81C_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS5063623.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14378..14758)
                     /locus_tag="ACG81C_000433"
     CDS             complement(14378..14758)
                     /locus_tag="ACG81C_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5063624.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(14762..14977)
                     /locus_tag="ACG81C_000434"
     CDS             complement(14762..14977)
                     /locus_tag="ACG81C_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063625.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(14980..15387)
                     /locus_tag="ACG81C_000435"
     CDS             complement(14980..15387)
                     /locus_tag="ACG81C_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS5063626.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(15653..17128)
                     /locus_tag="ACG81C_000436"
     CDS             complement(15653..17128)
                     /locus_tag="ACG81C_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS5063627.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17118..17966)
                     /locus_tag="ACG81C_000437"
     CDS             complement(17118..17966)
                     /locus_tag="ACG81C_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS5063628.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18003..18365)
                     /locus_tag="ACG81C_000438"
     CDS             complement(18003..18365)
                     /locus_tag="ACG81C_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063629.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18366..18524)
                     /locus_tag="ACG81C_000439"
     CDS             complement(18366..18524)
                     /locus_tag="ACG81C_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063630.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(18665..19289)
                     /locus_tag="ACG81C_000440"
                     /pseudo
     CDS             complement(18665..19289)
                     /locus_tag="ACG81C_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19286..19597)
                     /locus_tag="ACG81C_000441"
     CDS             complement(19286..19597)
                     /locus_tag="ACG81C_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063631.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(19641..19934)
                     /locus_tag="ACG81C_000442"
     CDS             complement(19641..19934)
                     /locus_tag="ACG81C_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063632.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20128..20775
                     /locus_tag="ACG81C_000443"
     CDS             20128..20775
                     /locus_tag="ACG81C_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5063633.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            20836..21981
                     /locus_tag="ACG81C_000444"
     CDS             20836..21981
                     /locus_tag="ACG81C_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5063634.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22062..22367)
                     /locus_tag="ACG81C_000445"
     CDS             complement(22062..22367)
                     /locus_tag="ACG81C_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5063635.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22392..22823)
                     /gene="ruvX"
                     /locus_tag="ACG81C_000446"
     CDS             complement(22392..22823)
                     /gene="ruvX"
                     /locus_tag="ACG81C_000446"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5063636.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(22820..23086)
                     /locus_tag="ACG81C_000447"
     CDS             complement(22820..23086)
                     /locus_tag="ACG81C_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5063637.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23341..24066)
                     /locus_tag="ACG81C_000448"
     CDS             complement(23341..24066)
                     /locus_tag="ACG81C_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5063638.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24427..24497)
                     /locus_tag="ACG81C_000449"
     tRNA            complement(24427..24497)
                     /locus_tag="ACG81C_000449"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24463..24465),aa:Gly,seq:ccc)
     gene            24614..25366
                     /locus_tag="ACG81C_000450"
     CDS             24614..25366
                     /locus_tag="ACG81C_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5063639.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25382..25861
                     /locus_tag="ACG81C_000451"
     CDS             25382..25861
                     /locus_tag="ACG81C_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5063640.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26112..27692)
                     /locus_tag="ACG81C_000452"
     CDS             complement(26112..27692)
                     /locus_tag="ACG81C_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5063641.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(27685..28428)
                     /locus_tag="ACG81C_000453"
     CDS             complement(27685..28428)
                     /locus_tag="ACG81C_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063642.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(28604..29380)
                     /locus_tag="ACG81C_000454"
     CDS             complement(28604..29380)
                     /locus_tag="ACG81C_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5063643.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(29628..30464)
                     /locus_tag="ACG81C_000455"
     CDS             complement(29628..30464)
                     /locus_tag="ACG81C_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5063644.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30475..31404)
                     /locus_tag="ACG81C_000456"
     CDS             complement(30475..31404)
                     /locus_tag="ACG81C_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5063645.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            31596..31949
                     /locus_tag="ACG81C_000457"
     CDS             31596..31949
                     /locus_tag="ACG81C_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5063646.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32023..32679)
                     /locus_tag="ACG81C_000458"
     CDS             complement(32023..32679)
                     /locus_tag="ACG81C_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5063647.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            32905..34839
                     /locus_tag="ACG81C_000459"
     CDS             32905..34839
                     /locus_tag="ACG81C_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5063648.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(34925..36148)
                     /locus_tag="ACG81C_000460"
     CDS             complement(34925..36148)
                     /locus_tag="ACG81C_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5063649.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36142..38013)
                     /locus_tag="ACG81C_000461"
     CDS             complement(36142..38013)
                     /locus_tag="ACG81C_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5063650.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(38034..39152)
                     /locus_tag="ACG81C_000462"
     CDS             complement(38034..39152)
                     /locus_tag="ACG81C_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5063651.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39235..40068)
                     /locus_tag="ACG81C_000463"
     CDS             complement(39235..40068)
                     /locus_tag="ACG81C_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5063652.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40061..40978)
                     /locus_tag="ACG81C_000464"
     CDS             complement(40061..40978)
                     /locus_tag="ACG81C_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5063653.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41205..42329)
                     /locus_tag="ACG81C_000465"
     CDS             complement(41205..42329)
                     /locus_tag="ACG81C_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5063654.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12696 a         8587 c         7056 g        14045 t
ORIGIN      
        1 agtacggcat catctgccgc actcctattt ttttcgtcat ttgtttctta aataaaacga
       61 ttctcacgca attttatttt tttgatatct tctactgttt gtgttccagc taattgcatg
      121 accatttcca attctgtttt aaagaaatca aacacttgat ggactcctgt acttcctcct
      181 aaagacaatc cataaataac gggtctgcca atagcaacaa gatctgcacc tgatgcgatt
      241 gctttgaaaa catgctgtcc gcgtcgtaca ccgctgtcaa acacgattgg tacacgttta
      301 tctactgctt ctgccacata ttgcaacgaa tcaaacgctg ctggaccacc atctagttga
      361 cggccaccat gattagatac ccagattccg ccagcaccag attctaatga acgatagaca
      421 tcttcttctg actggacacc tttcacatag actggcaaat cagaatgagc agcaataaat
      481 tctacatctt ttggactaag cttctgtttg gaagatttgt acacagcatc catcgtttgg
      541 cctactcctg attgataagc ctgaacgatc ggcattggca gtgggaaggt aaacccattt
      601 ctgcggtctg tttcacggtt tccgccaact gttgcatccg ctgtcaatac gattgctttt
      661 gctccattac gcttggccac ttcaagaata tccaaattga tcccatcatc tttgctcata
      721 taaaattgaa accattgcgg tgctttctcc ccgcctgctt ctcttatctc ttctaatgta
      781 caagaagcgt aagaacttgc tgtatagatc gtcccaaaat ctgctacacc ttttgcagaa
      841 gctttctccg ccttcacatg agctaagcca tgagcagcaa ctggtgccat gatgattggt
      901 gcagtcagcg tttctttgtc aaaatgtgta gttgtatcgg gtaattctac atcttttaat
      961 acatgaggaa taatcagctg atgattgaat gcccgttcat tttcttgata agtaaataag
     1021 tcgccagcac cactgctgat gtaaccataa ccaccagctg gaatcacttg tgaagcggct
     1081 atttctaaat cttccatatt gataaaatcc actattcctt catttgtacc tgcttgatac
     1141 gttttttcca tttcaatgcc ttccctctat aaaaaattga atgaataata tgatagcgtt
     1201 tacacacttt atattactct tccgtattga tacaggcaaa taatctgttt acatttcagt
     1261 cattccagtt gttactactc tgaaagaaat gtttagaaca caaatagata aaaaacgatg
     1321 ggactttgac aaaagtcgtg tcaaagtccc ttttacgaaa tactgtggat gcctattgaa
     1381 ataagtgcgg gttgtttctt tgtatttcaa tcgtttcttg ccattcttcg tttaattttg
     1441 gattaccgtt ttcttccttg aacgcataag gtcccaatcc aaaaagctca gttagtattt
     1501 gattcacgat tccatatagc tcggtgtgat cggacacatg attattgaag atcatctcat
     1561 tatcaccacg tttgaatctc acattcgggc tctcatgatg aacctctatg cagttttccc
     1621 ctttttttaa ctgacggatt tctgaaaaca tctcataaat atcttctcca ataaaacctt
     1681 cagcaagcgc cagactgtcc acaagaccgc ctactgtcaa ctcagagatt atcgctttgt
     1741 tggcaccaag ctttaatgta tagcttgttg cttctactac gccgcatgca ttgcaaccac
     1801 cttctgatag ttcgcttttt ctgatcaatt gaacttcttt cattttctgt tcctcctctt
     1861 agttttctac ttatgtattt tttatcacct gacgtaagtg tattttatcg tcacgatcac
     1921 gtgatttatt cttcagagat tctatttatc tatctaacat atcctttatt cttctacttt
     1981 tctttcttta gcatataaga gatacgcata gtaatcaagc aaggattgtt gatataaaaa
     2041 gaacaaatac cgctattttg atttcttttg actctattac tcatataaat aaaatttatt
     2101 atacctgctc tttttgacta tatcctcatc tgactcctat acttcgtttg tattaaaact
     2161 taggaggaat cattcatgct tacagggcta aacttattaa ataatccttt tctaaataaa
     2221 ggaacagctt ttacaaaaga agaacgggca aaatacggaa tcactggtat gcttccaagt
     2281 actgtccaaa cattggaaca acaatctgtc caagcttacg gtcaatactt aagcaaacag
     2341 accgatttgg aaaaacgtat cttcttaatg aatctattca atacgaaccg gacattgttt
     2401 tataaattga tgggcgaaca tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt
     2461 gcggattcaa tcgaacaata caacgaaatt ttcttgaaac cacaagatgc tgcatttctt
     2521 tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa acgcagcaga tggacgcgac
     2581 attcgtttga tcgttgtgac agatgcagaa ggaatcttag gtatgggtga ctggggtgtg
     2641 aacggtgttg acatcgctat cggtaaattg atggtctaca ctgctgcggc tggtatcaat
     2701 cctgcacaag tcttgcctgt atcaatcgac gctggtacta ataacgaaga attattgaat
     2761 aatccattat acttagggaa tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc
     2821 attgatcaat tcgttcaatc agctactgaa ttattcccag aattgttatt acattgggaa
     2881 gattttggac gaggaaatgc tgcaacgatc ttagaaaaat acgaagataa aatcactact
     2941 ttcaatgatg atatccaagg cacaggaatc gttgtactag ctggtgtatt aggtggattg
     3001 aatatatcag gtgagcaatt gaaagatcaa acgatcttga catttggtgc tggtactgct
     3061 ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa
     3121 gaagcacgcc aacatttcta tcaagtagat aaacagggtt tattgtttga agatacaaaa
     3181 ggattgactc ctggtcaaat cccattcgct cgtaaacgct cagaattcac taatgctgat
     3241 gaattgacaa acttagaagc agttgtaaaa gcaatccatc caacgatcat gatcggtact
     3301 tctacccaac ctggagcatt cactgaagaa atcgtgaaag aaatggcggc tcatacccca
     3361 cgtccaatca tcatgccttt gtctaatcca acaaaattgg cagaagcaaa agcaaaagat
     3421 ttgattgaat ggacggacgg aaaagcttta gttggaacag gtatccctgc tgctgatgtg
     3481 gaatataacg gtgtaactta ccaaatcggt caagcaaata atgcgttgat gtacccaggt
     3541 ttagggcttg gtttgattgc ttcaacagct acacgagtaa atgctgaaat catttctcaa
     3601 gctagtcgag cattaggcgg aatcgttgat gtaacaaaac caggagcagc tatcttgcct
     3661 ccagtagcaa aaatcactga attctctcaa acgattgctg aaactgtcgc aaaatcagtt
     3721 gttgcacaaa aattgaatcg tgaagaaatc acggatatca aagaagctgt tgaatctgct
     3781 aaatgggttc ctgaatataa atctttagaa gattaagatt gttatagtaa aagaacatac
     3841 agaaactttg tatgacgtct aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca
     3901 actactgctc ttttgccgct tctctttggc ttttttcata cgatacagaa taagaaagga
     3961 tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa
     4021 gggttttggt caacaaaaat taccggcgta agtttgcccc tttatttgat catggtcctt
     4081 gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga atatgattgg cccaatcgct
     4141 gttttggtca ttttcggtaa tttatttcat tttatcggaa caaaaatccc tatcgtcaaa
     4201 agttatttag gtggtggctc cgtttttgcg atctttgcct ctgccgcgat tgctacattt
     4261 gggatcttac ctgagtatgt agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt
     4321 ttagactttt atattgctgc cttgattact ggtagcatcc ttggtatgaa ccgtaatcta
     4381 ttgatgaaag cttctgttcg ttttattccc gttgcactta tttcaatggt cgtctccttt
     4441 tttgccgtag gactcgtagg tatgcttatc ggaaatggtt ttgcaaattc cgttttatat
     4501 atctcattgc ccgcaatggc tggtggtgtt ggagccggag ctgtaccttt atcaacgatt
     4561 tatgcaaatg ttttaggtac ggacgcttct tctatcattt cccgcctgat tcctgcgaca
     4621 gcaatgacaa atgtcctagc tatcattggt gcagcgttag ttgctcgtgc gggacaatca
     4681 atgccaaaat tcaacggtaa tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa
     4741 cctagaaatg taaaaccttc cattccacaa ttaggtgtcg gtttgatggt atcattgact
     4801 ttcttcatct taggacaaat ctgtaactat tttgttccta aagtccatgc ttacgcattc
     4861 atgatcatta tcgttgttat ctgtaaaatc gcaaacctat taccagaata ttacgaagat
     4921 gcagctatca tgttcaacaa cttgattgtc aaaaacttga cagcagctgt tcttgccggt
     4981 atcggtattg cgttattgaa tctaaatgta ttggcttctg ctttgacatg gcaatttgtt
     5041 gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt caggatttgt cggtcgtttg
     5101 tttggtttat atccaatcga atcaactatt actgctggct tatgcaacaa ctcgatgggt
     5161 ggtacaggaa atgtagctgt cttatctgca gctaatcgga tggaacttat tgcttttgct
     5221 caaatgggca atcggcttgg tggtgccatc gttttgatca tctcaggttt cttgatgcaa
     5281 ttactttctt agtcaaaaaa aatacctgat catcaagtat cggcttgatg accaggtatt
     5341 ttttgataaa aatttacagg attttcaaaa atcttttttg cattttatcg gaatgataac
     5401 cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc tgcagaatta agtcttattg
     5461 ctgatattcc tcgtcttttc gcttcttcta cgccagtcat caaggccttt ccgattcctc
     5521 ttttttcagc atgtttagac acagcaagtg ccataatatt gaacatcgga tcgctgaaaa
     5581 tcgattcata ggactccgct tgtacatagc cagctgcttt tttagaagta ggatcttcat
     5641 agaccaagaa aaaatttttt tctggctcct tcaacagttt cttcatttgt ctcaccgtaa
     5701 cggttaatgg tacatcatat cctaattgtt ccgcattgat ttctcgtagt gcttctgcat
     5761 ctcctaaacg catttcccgc aacatccaaa tcaccagcca atttttattt ttttaaatga
     5821 atttttttat cacagtatac tccttttttc atttagaaca atatgtttca gtgattttga
     5881 cttgcgttag tctagattct cctaatgttt gtaagatgac gaaagcattg taaagattga
     5941 tcaaaagaac cacgaggaca attacttgaa aaataaaggt cacttttttt ggagacaaaa
     6001 ccctgctaaa tcttgctccc cacaaacctc caataacagc agcggggata acataaaaca
     6061 acatagataa atcaaagcgc ataaagctac ttgttaaagc aatcgttacc aattttgcca
     6121 gttgagaaaa gaaaatcgta gatagagaat atagtgttgc ttcttttatt ggcaatgcaa
     6181 acatcagcat caacaaagaa acattgactg gtccaccacc aatacctaaa aaactagcta
     6241 aaaagcccag aaccccccca catataaggt accaagtcca tgacgtcaaa tgaaattttg
     6301 gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt
     6361 ggatcagctg cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt
     6421 tgccgactac tcctccaaca acagctccac tagataccca accgacaatc tgccaattca
     6481 gtgatttccc gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa
     6541 ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc gataagatca agaatcggtt
     6601 tgatcaacac gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc agaacgatca
     6661 ccacaaaata aatcagacca atcatcttgt atttcctttc tcttgtttat atcttttagc
     6721 gattgttact cttttctttt attatacagt aaaagaaaca aacgaggctt cgaaagcttt
     6781 tctacttttt aaggaaattt agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca
     6841 tcaagtaata agaacagcca aacaaaaata gctcctcata attttcgttt ggttgaactt
     6901 attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag cctgccgttc
     6961 agctccgagt cacaaggaac aatttcaaaa aacagcttct catgtttgtt gaatattgtg
     7021 acttgtgacc gaggagttgg caggagaaca ccgtttaagg aagagactgt gaagtcaacg
     7081 ttttgacctg attattagag aaacaaatgg caaaatagtt cttcatattt cgccgttttt
     7141 cgagcgaatg ccgaggggca gctgattgaa catcgtttat ttggaaaaag ggattgtgac
     7201 aagacgtttg tcacaatccc tttttttata tcttattaat tcattctttt gttctattct
     7261 tcttcttccg ccatgaatga gaaacccata tcaaagaact ataaaggtta tcaaaacagc
     7321 gtttatgact aaatgaatta gagttatcta tcactatttt tcaagcgatt taaaccaaat
     7381 ggcatacaat atggcataca aaaacttcaa taagccgtgg gggtttttaa atttcttctt
     7441 gttctttaaa atcaatgagt ggagctacat aatttgaatc cactttttca aacgtcactt
     7501 tttgcatcga cgaatgtttt ctttcccaag ataacgtaaa tagatttgac tctggactta
     7561 taaagtctac ttcaccttct agtattactt tcttatgcaa atattcctct aataaatata
     7621 gacttgctaa agcatacaac acattttcca aatttgcttc cttataattt gaagaacgac
     7681 catgttttac tctgttatat attttccacc aatctggtgt atcttcaggc cacccatctc
     7741 caaaaggttt tagtgaagtc aatgttaaag aattcataac cctaacacca ttatccaaac
     7801 acattaattc gctagattta ataacagctt taaaatcagt tatgctttta ttgctactgc
     7861 tatcaaatcc gcaaagttct ttgaaaataa tctcacactc cgaaccaatc gctagtagta
     7921 gtttttgata ttcaatagag aatgttttga aattattctc atcgagcatc acaaaatctg
     7981 tggtattaat aaactcattt tctaaatgta gataatactt ccaataaaaa tttgtaaact
     8041 ctgctttgtc cattaaatca cctcttttga attataacaa aaaaaacacg tccacaacag
     8101 acgtgttctt tgatttctac acaatcagct tgccaaaacc atccaatcag tcctagcatg
     8161 cctgtattaa aattgaacgt tcatcatcta tcatctttct tagtccagca tgtttgcaac
     8221 cacacctaca attttcagtc caacaataga tttcgactag ctaagtcttg caaaattcag
     8281 gcagctaacc cttcatttac taccttaatt atactaaata cccacaacgt ttacaagtgt
     8341 aatcattcta agaaatctgt ttagctatga tcgttgtata atctttaaac gccgttcctt
     8401 cattgattga aataatttta taactcttgt ttttccaagc tactttcata tcgtttgtta
     8461 gttcataatc ttgatcgtat cgaataataa acgtcaacgt tccttcaaga atggtaccta
     8521 cactagcaag cacgtctttt agcatttgtg aacgcacatt agcccaacat tcaaatacag
     8581 tttcttcact atccaccatc tgcccatctt catcttttac ccgttttgtc tttttaaact
     8641 tgactctctc agtcaattca tttacattct taatgattgg cataataagc acctctcatt
     8701 tgttgtacta atgcttgtac cgttactggt atccgttcgc tgctggcttc ttgacggttc
     8761 aaataccaat gttgagtaag taatgacacc gccaaatcaa attgctgata attatccata
     8821 tctttatccg ttgcgctact atcaatagca ctttttatat attcgcctgc tgtatcaatc
     8881 aattgttgaa taaagtcatc atcaatagtg tgatcgatcc gcatactttt ttttatgcta
     8941 tccaaaatta ccattttatc tctcctaatg aaaaaaaggc agtgggataa accgccgccc
     9001 aatatttttc tatacttttg gtgttacaac tggcgtaaac tcaatataca ctgaagcatt
     9061 ttgatcaatt ttcttataat cgttgcgaac gatcactgca agtccttgtg agtagtaatc
     9121 gaatttttcc cattgagtcg ttacttgatt tctacgagca acaaagatag attgtgcaat
     9181 atcccccata atcataggga atgttccagc tttaggattg gcaaacaatg tatctgcaat
     9241 caatactact ggcataccga ataatgattt accactaggt gctgtcacat cgggttgtaa
     9301 aatgtaacgt ccatctgaat ctttcaatgt atctaggtgg taatatccgc tttggtttag
     9361 aattaccatt ttatttaatg ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc
     9421 caacgtagca gccgttttct tagtgaatgt ttttaataaa tccatgatat gcttattgtc
     9481 agtgttttct accaatttcg ctaactgatc tttgacctct tgtacaatat tcactgctga
     9541 atcttccaca acctcatttg ataaggcaat cttaccagca cgagtttcta ctttataatc
     9601 aactgaagta aacatttcag cgtcaatatc accaatttca gcaagttcag cttttgttgc
     9661 taacactgct tgttggttag ttgctactgg atatttacct tgtccatttg atactgtttt
     9721 tactgttgca tattgagcta agttataatt tgaacgtttc aaatcaaaga cttcaccgat
     9781 tacttcttcg ggaacaactg ccgctgcatt tacagtagtg actccatcac gcacttcgcc
     9841 ttgtgaacgg atatattctt catacccacg caattctgtt ttttcgttat cgattaaagt
     9901 tttttccatg ttattactct ccttttgtgc tttagaagtg gattctaagc tattgtttgt
     9961 ttgttttgct tggttgttac tcataaacga ttcgtaagaa cgcttattta cttggacatt
    10021 tgatgaatca taagcgggaa cggtcacgac actaatttca ttaagtgctt tgattttatc
    10081 tattgatcgt tcaattgtgc catcttcttt tttgtcgaat gaatctaatc caagcacgaa
    10141 cccaaaggac atagaatcaa caaccccttt tgagatattc tcatacacat catttgaata
    10201 ggtcgtgtcg gttagctctg cttcaaaatg taatcctgta tcatcgacat tcaatttcaa
    10261 cgtacctgct ttgacactag ctagtggctt tgaataatcg tgtccatata ataagaacac
    10321 atttgataaa tccacctctt ttaatgcttc gggtgtaatc acttctacaa agccgcctaa
    10381 atcttttgac ggttgcccga atttcaacgc atagcctgaa atagttttgc cgtctgttgg
    10441 ttgtttttca ttttctttct ctactttggg agaatcggct gttagctcag cttcttctgt
    10501 cagccgtttt tccttttctt cttcattatt caactggtgt aactccttcc tgttctttta
    10561 attgatattt ctccaacgta tctaaatacg taaagttcaa acttgctagt aaacgatcgc
    10621 caccatccat ggggggtagc cccattttcg accgcccctc attaatcgtg agaagtgacc
    10681 ctttgacctg atccaataca ttttttactt ttgtttcagg atcagtctct agtaactgat
    10741 cggaattaaa gcggatattg gtttcaagtt tcgtatctaa ttcactcaca aacacattaa
    10801 agtaatgaat cagcgtactt tggatatact ggagattact ttgaacggtg ctagaatgtt
    10861 cattttcaac gcctaaacgc tcaatcggta caccaaacgc cttggcaatt tgttttgtgt
    10921 tccaatcatt ggaattgata agcttcaaaa catcggtgtt tatttctaat gttttgtagt
    10981 ccattgtttc atcaagaatg atcgttctaa gagcattatc tccactactt gaaccgttgg
    11041 cttcttcaaa cttttcacga attgcgttct ttgctgatcc gtctaaatct gacttatgaa
    11101 ccttcaaaat acctgaccca ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc
    11161 cagcttgttg aattttcagt tcatcacgta acgcataaag tggtggtaat cctgttaaac
    11221 catcttgggt gaaatatttg aagtgcaaaa tatcgctaga cttcacacgt ctttttttat
    11281 caccaatctg ataagacaac gtaccattat ctaattgagt aaccgttaca cttgagttag
    11341 gtaataagtg aatttcttct accttttcac cgttgcgttt aatctctgca aagctattac
    11401 cgtttagcaa catattgacc gccaaagcaa actttaaatg ccaaccatcc atttctgaat
    11461 taggtttctc gtttagtaac ttcaccagtt cattatcagc ttgtggcata ttgtttttga
    11521 ccaattgaat cggacttgaa gcaatatcac tagcaataat tcgaacggct gcaaacacat
    11581 cactatttct taatgcacga acgcttgtaa agctggtggt gtaatcatcc gattggattg
    11641 ataccacatg ttgtaggaaa ggatctcccg ttgtattttg tctcgcttta aaaaatgcca
    11701 tcttttaacc tcccttctgt tctgtattta gcatgaccgc taccccaata agcactatgc
    11761 ccagcgctaa caagccatag tagacatttg ttagtaaggt gatagcaacc aacacacaca
    11821 ccaatccaag aagtaaaaga acggtatgaa tgtaattaga aagaaaattc ttcacttgta
    11881 taaaattcat tgtctgcttt cgctccttcc gttgtggtga agtaatccat tgcaaacaca
    11941 taggcattga ttaatgcagc tattggatca attttgttac tgttctttga tttattaatc
    12001 tgaatgccgt tgttatccgt ttttaaaata gcgttgttca ctgcatgggt gagaatcgta
    12061 tttttattgt gaataacgtt gccttcataa acttgttctc taaaagttcg ggtcggaacg
    12121 tttagagtaa tcgttccttg tcttacttct aacattgggt agttggcttt ttcagcttta
    12181 ctgataagcg aattagcgtt atacgggtca taacaaatcc ccatacattc caaatcattt
    12241 tcttgaatca aatcttgaat aaactgaaat acttggtcat agtccacgat tccactttct
    12301 aattgagtaa tggaacactc accagctttt tctagttctc gataaggcaa accgtcgcgc
    12361 ttttctttgt cttgaagccc atatttggtg gctacaaagc tatgtgaatc acaataaagt
    12421 tcgccgttat ctagcggtac gatccatgaa acgctagtta aatcatctgt tttagataaa
    12481 tccactccga tataaacggg cttgcctgta atatcttgtg gttctacttc ggttgcctgc
    12541 cagtcttcag caatcatata gctgtcctca ctcgcttgtc tccataaatt gaagtttttc
    12601 accaatacag aattaaggtt attttgtttc aaagcaaggt tcacatcatc ttgaatggtt
    12661 ggaatcatta cttttttgat ttcttcactt tcaaaaatcg gattggcttt gatccatttc
    12721 tcttgatcgt gaatttcttc gggatcgtcc aattcccata tcgctataaa gtacctgtcg
    12781 gcttgttctt cgccttttag cacacgatct aacaataagt attcctcgta cataggaacg
    12841 tttaaatcaa gcccagcggt actaataaca cacaataagc cgttcttttg ttgggtcatt
    12901 cctgacttaa tgacgttgta cgttttacga gtttttgctt cgtgccactc gtcaagaatt
    12961 gcggttgttg gtgcaaaccc atctagcgtg ctggtttcac tagccagtgc catagcgaac
    13021 gaattagagg gcaagtgctg aatcttagag ttcataattt tcagttgtgg tcttaaaaac
    13081 ttactagact tgaccacgtt tctaagtgaa ttagacagca tatcatagcc caatttagct
    13141 tgtttcaaag cgttacttac aaacaatact tgccttgctt ctgctggttc ttgttccata
    13201 atcaacgcat tggcagccat gccacttgcg atatacgttt tcccattctt acggctcata
    13261 ctggtaaacg ctcgattgaa tcgcctgtaa ttcccttctt tagtacgcca gccataaaga
    13321 ctgcctagaa tgaatttttg aaatcctaac atcttgattg ctttaccgtc cgtagatggt
    13381 agcaactcaa tgaatttaat cgctttggtg gcttgttctt catcgaacac ataagggaag
    13441 ctatctgact tagaacgctc caaatcgctt atatgacgct cacacgcttg gataattttc
    13501 ttgggtgcaa tcagcttacc tgataggact ttctcaatgt aatcattcat gaattcatca
    13561 actctttgaa cgggtcttcg ggttcatcat ttttaacctt gttcaaagct agttttgatc
    13621 gtgattctaa ggtcatacct agtgaggtgg cttgtttttt caaatcactc atagcttgtg
    13681 attgaacccg aaccgctgga ttggctttct ttactccgtt ttcgcatgtg ataaacgtac
    13741 ctgattttct tatctcggct tcggctgttt tcattcgagc aaaggctaaa caataggcta
    13801 ccaatgaacc ataatctgct tcactcaatg gataatcttt ttttaatgtt ggtacgatac
    13861 gattccattc acttctagcg ctcgctgcca tccagtcagg gggcgtgata tttattaatg
    13921 gttgctgctt gaacaattct tctctagcgt ccacacggat tgctttttct tcacttgata
    13981 tattgccttt tgtatcgtgt aatattttag ggtgtcccat gttgtcctcc tttcagacaa
    14041 aagaaaaagg aacgtaagca attaagctca cattccttcg tttttcgatc agaatttttc
    14101 tttatctaat ttatctatat aaatgatttt tccgttctgc atttgaatgg tcacttgacc
    14161 aaaatgaggt aactcaatgg tgcgctgttt ccctgtttta tcagtaataa caattgtatt
    14221 tttcaataaa tctcctactt tcattatata ctttttcact tgtgattaca agtgtaatcg
    14281 ttatttgtaa ttacccttat gccttttcac aacggttatt tgtaccaaaa tgagtaggca
    14341 tcgattgtca gtaagtttca cactatgccc ccttaactca aagggtactc ttttctcgtc
    14401 tttgtctttc attggctgtc ttggaattat ggtgtgcatg gcataaagaa cgaaaattag
    14461 tttcatctaa ccgtctattc caatcatctt ttagttcaat tttgtgatct acaacatcag
    14521 ctttccttat caagccttct tctaggcaat cctcacatac tggatgattt aaccgataca
    14581 agtatgaagc ctttcgccat gttctacttt gatagaattg aaaatattta ccttcgcttt
    14641 gctttctttc ttcacgctta attgccgttt gctttgcttt atgcttccca caatacttct
    14701 gcctataatc aatcaatgtt ttacatccag catgattaca ttgtttttta actgccatag
    14761 attaccaacc aaagcgatct cgttcgtcag aatcatcttg ttcccatttt gatttgtatt
    14821 caatataagg cgaaactatc ttcaagcacg ttccgtcttt aaaattaact ttagtgattt
    14881 tatcttcctc aaaactaata aagtctacat cttcttgagt gtttgaatac tcaatgtgtt
    14941 tctcaccatc gaagtatcta atactttcca tatgcataat tattctacct ccaataaatt
    15001 agttatcaat tcatcatctg ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa
    15061 caaattatcc tcattcgatg taagctctcg ttgtctcgct cgatcatata ggttaagcag
    15121 acaattaaat tcatccatta gctcacctgt atcatcatag ttgccactat ctaccaatga
    15181 tcccactact agcgattgac accacgctac tgactcctgc agccaatcag gttcttcttc
    15241 cattatcttt aatacttctg gatcaaatgt ggaataatct tctgttacca atcccacact
    15301 aaatgccagc gtgttcatta gaaaccgatc catttcatct gtatattgaa agttcggtgc
    15361 tttgtttgtt gaaataatat tgagcattta ctttcctcct tgattcgtaa cacgtggtaa
    15421 cacctacgta acaggtaccc gttaccactc aatcccaacg gggacaaggg ttctagcgtt
    15481 ttggtaacag gtaacgggta aaatccagta acattattcc ccagcatatt agctaactat
    15541 ttcaaaagtt ttaaggaaat aaggtgttac cccgttacca aattgctgta tcccttgggg
    15601 cagtaagggt aaactagtaa cgggctaggt gttactgacc cgttactacc cgttactttt
    15661 tgtactttcc agctcgattt ttattttcat aggcatttga cttattgtaa aatcctcgtc
    15721 gtttatttct ttttggcagt tgcttccgtt cccaaccagg aatactgcta ataatcaagg
    15781 caatcttttt agtttctgca tctgatcgac cattcaataa atcctttgct tgtttatcaa
    15841 aaacagcttc caaaatatca gcggtgagta cgctatcaac taaataaaac tcttgttgca
    15901 tagtagctcc attatagaaa tgctgtaaat ttttctcatt acatttactg tcaggatagt
    15961 tctgataata agaacgtctc gcccacgctg gagcttcttc ccatttatac gggatttcca
    16021 tttccaaata tttagtgatt gcttctttaa ttggattctc catcttggca tcctcttgat
    16081 agtcttttgc aatttctaag acttctttat cttcatcggg actaaaatag attcgttgtc
    16141 ctttatcaaa aagcaccttt gcttcagcta acacttgaag aaaatattca tcctttgttt
    16201 taaacggatt catctttggc ttattcttac aaggcaaagg ataaaaacgg cggtttcctg
    16261 tatcatcttt caaatactgc ccatcattcg acgttccaat aaatacacat tgccttgccc
    16321 aagctatgac attccgacca taaggaggtc taattttatc gatcttggca ctgatgaagt
    16381 tttttatctg ttcaatttta gttttgttca ttgaagataa ttctccaagt tcaataatta
    16441 cattgccttg aagctgcata taatcatctt tcttttcacc tagcgaatct aatgaatcgg
    16501 tgaaaaaatc atccgtgtaa agtaatgctg gaacggtact cttaccaatc ccttgcggtc
    16561 catccaaaac tggaacgatc tcaaacttta ttcctggttt atagatacgt gcgactgatc
    16621 cgactatcca acgttttgtt acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt
    16681 caataaacag tgtttccagt ctaggctgtt gatcccattt aactttttcg atacgttctt
    16741 ttactgggtt gaaaacattc tttctagcca catttactac tgctttgtgt attttgttgt
    16801 catcaaaaac tacattgtag ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt
    16861 cttcccattc tccagcggta cgattgatcc aaggagaatc ttttaatagg tgaatgtatc
    16921 cgctaaattc gttataagca attgttccag ctaaattgct atcattggtt aaaatcaatt
    16981 ctgcattcac aacactgtta cttttaatct gatcgtcttt attgcatctt aatcgtgaca
    17041 tccaggtttt caattcaatg atattgtctt cacgctcgct ggcttcggga aagtctaaaa
    17101 tactatctaa tttatgctta tgagccatga gctttccccc tcaaatcttt tttaatcatc
    17161 gatcgaataa tttgattcac ttctttatct tttagcggtg gttcattgta cgcatttgct
    17221 gtataaatca attctgctaa agtttgatta tctacaccgt gccatagtaa ccaaccgcat
    17281 aacttagtta aatgatcgtt tctactacct tcgccagcgc cttgaacaat catatctaat
    17341 ttttttgctg tccctgtttt tgctacgggt cggtattgag tctttatttc ttgcggtttt
    17401 tttagcagta ggttcactag ccaatcggga gctggtgcaa tattatttag agatacgttt
    17461 tcaataggtt catatgctct cccaacactt cttgaaataa tcatagttgg cgctattgga
    17521 actcctttag tgataatatc aatcccgctt aaacttttac ctttatcatc acgcccgaag
    17581 atatcagcaa atgcacctgt ttcttgttgg ataattacac catcaggaat tttaaagaag
    17641 aaatggattc ctttacctgg tgtgatttcc gtataagtag aaggaaatgg atcatacatt
    17701 gaagcaagtt ttttataatt ctcaacgccg tttcgtgttt cagaatgttg atctaggtca
    17761 actaccacca tttgatgatc ttcaagcatt aacccaatat ttctagttgc atcttccgtc
    17821 caccattcat taatttgatt agaatcgtta gttgcatcat tccaaccact agagcctttt
    17881 attggaattt tagttccagg agataaggga taaattgacc atcccgactt ttgtaattcc
    17941 gttgctgctt gataattatt tgccaataaa cctctcctcc tttttcaaaa tactggtata
    18001 aatcattcag acacctccat gagcttcaac tgctcaagtt cgtatttcaa cccatcccga
    18061 acaaattcag caccttttaa ttgattctga tactttaccc atgtattaaa tgttttcacc
    18121 cttgcttctt gcatatcagt aatgtttaac catgcggtta aaatctgatt ttttagctcg
    18181 ttgatttctc gacaaattat tttgtgttct gcatgttttt tgagtacata caaaactgtt
    18241 agtttttcat tgaatgaatt tccaaagtag gttttcccga tattattttc tagttcagcg
    18301 atcccttgat taatttcctc taattcaagg ctatcgagtt ggtttaatag ttttgttttg
    18361 tacatctact tatgccctcc agttcttaat tgatagtcgt actcgtcata agcagtgaag
    18421 agatataacg ctaatcctgc aaagatagct atttttgcaa ttaatggtaa taagccgaaa
    18481 attccgccga ttaaaatgct tcctataatt aagtttcgtt tcataatggt tccctccgat
    18541 tttttaatat ggtataatcg gaacaaagaa tatcgggttt tatcccaatt aagttgcctt
    18601 tatcctgtcc gccaagactg ttatggataa ggctttttct ttgttctcga tcatgttaat
    18661 gctcctaagt tgtctgcaat tcagcagaac gattataaat tttctcactc actgaaaact
    18721 gcaacccata tttttcttta acagtcatta attctactgt ctcatctaaa ataggttcac
    18781 gatcaaccaa catttcagga gtcatatctt tcttttttac cattttggaa tgaccataaa
    18841 tagttgatac agctttattg gctatcacat tcgctttaat gtagtctact ggttttggtt
    18901 cagtaatagc gtggcttaat ttagtcattg cttctttttg gtgttctttg tctagcatgc
    18961 ggaatacttg aaagccttca agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa
    19021 tccagtcttg aaatgcctcg gcttcttttt tacggctttt gaatgccatg cggtaaatat
    19081 tttttttcgt tgatgatgtt tacatcttgt gttctgccca atgaatcgat gaccttacta
    19141 gtagtaaccc catctttagg caaagagtga atggctctag taacctgttt aagacctaac
    19201 gcttccgcaa catctttcgc cactgcccac cattcatcat taatatcgac aaatcttata
    19261 atgtgtccgt tccaaatttc agttttcatt caacgtcctc cagttcaagc cctagcaagc
    19321 tcgctaacaa ctcaacttgc tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt
    19381 ctttaaactc tgcttgtact aaatcgttca agccacgcaa tactccgctt accgttggtt
    19441 cttgtggtag caatttttga tagtccattt gttcgtacaa acattcaaac gtttcttcta
    19501 gcgctttgcc ttccaagttt gctaattctg ccgctgtgtc ttctgtaaga attgttgata
    19561 atactttttc gattgcttta ttttgtgtcg attccattat tatttcctcc tattaatccc
    19621 tttgccccca cggcttgcgt ttatttttgg tattcttcca taaagcgatc acattcatct
    19681 tgtgaaattc ttacgactcc atcaactata atcaacttta aaccgttagg aacatatttc
    19741 tgcatcatag tattgtatga aacgttcata tacttagcag cttgtgcctt gttcatatat
    19801 tttggaaact ggtacttttt ttctaataat gaaagtaacg ttccttgagt ttgctctact
    19861 tgttgaacaa ctcgatcgtc aataatttta aaagctggat catcaagcgt gagtttgtca
    19921 atttttaatt ccatatttat cccccctcgt tagcatacat ttctatacaa agattttaaa
    19981 aaaatgcaag aggctcttga atacatttct atacctctta taaactcaat ataccatcat
    20041 accttttcat ttgcaagaat tttaacatac ttttttgaac ttttatgttt cacacatatt
    20101 taaatcgtga tatagtagag aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa
    20161 taccccgaag cagtggagac aggacaaaga attagactgg ctagaattga aaagggactc
    20221 tcaatggaac aacttggagg taaattatcc ccaccggcca gtaaaggtgc cgttagtaat
    20281 tgggaaaatg gctataatct tcccaataac accaggctta aacaactttc aaaggtatta
    20341 gatgtatcca ctacctatct attaaacgga tcttatacat taagagatat gcatatgatg
    20401 cccgaaatta agcgaaatgt gcttgaaaag caattgaaaa aaactattga aaaaaataac
    20461 tctataattg attctaatct ttctaatgct atcaataaca tcgaagaaga taacctgact
    20521 gtctcacaga agatttattt aacaaacgca ataaaatatc ttaaaacagg atctcgaatt
    20581 gaatttcctg acgggagtac cccaattgct tatctcgcag ctcttctaaa tacgctttta
    20641 cgatatgact cattaattaa tgattcagaa aaaaatacag atgagaaaaa agagtatcaa
    20701 gaattttatg aaaatgatct tctaaaagag ttcacagctc ttgttactga gctaaataaa
    20761 cactattcaa aataataata aactgcctcc tccaaccgcc cccacggctt gctacactat
    20821 ggagggaaaa ctattatggc aacattcaag caatatgaaa ctaaaaaagg taaattttgg
    20881 ctctatgaag cctatcttgg aatgaacaaa atgactggca aacctgataa ggttcgtaga
    20941 cgaggttaca aaacaaaaaa agaagcccag cttgccttgt cacgcttgca agtggactac
    21001 gataaaaacg gtattaaaaa agcgaacaac gaaacgttta aagaagttta tgacttgtgg
    21061 ctagaaacat ataaaaccac tgtccgagaa gccaccttca tgaaaactga aatcaagttt
    21121 agaaagtgga ttcttccaaa atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa
    21181 gcccaacaaa ttgtaaatag ctgggctgaa actactgacc aatacaaggt acttcattca
    21241 acttctaagc gtatttttag ctacgctatc aatcttggga ttattgagac taatccacta
    21301 gacaacgtat taatgcctaa aagaaccacc actaagcata aagaaaaggt caaagtttac
    21361 agtaaagaac aactgatcac cttgtttgat tacctaaaca gcaaacctga aacttatcga
    21421 aacgactttg ataaaacgtt actacgcttt ctcttttata gcggggttag gatcagtgaa
    21481 gcccttgcat tgaattggtc agacattgat tttcaagaaa atacggtcac catcaataaa
    21541 acactaagcc aatccaaaaa tggctataaa atcagtgatc ctaaaactga ttgtagtgct
    21601 ggggttcttc cactagatga aagaacaatt tctatattaa aaaaatggca gattaatcag
    21661 cggaaatata tgctgacact tggtgtaact gatccgacta tgattttttg tggtatctat
    21721 aaagaactaa tcacacacca cgctatttat gcaagattga ttactatttg tgaaaaagct
    21781 atggttccat ttctcggtgt gcatgtagca agacacactc atgcttcaat gctcttggac
    21841 gctggagcta ctatggtaga agttcaaact agattacggc atacaaaaat tagtcagacg
    21901 atcgattgtt acggtcactt ggcaaaagaa acgaaagaaa agactgttga aaaattagta
    21961 caacacttaa atattgacta gatggcatac aatatggcat acagtaaaaa ataaaaaaag
    22021 gagaaaccct atcatatcaa ggtttctcct agctttatag tctattcttc ttcttccgcc
    22081 atgaatgtat tgaatacttc ttcaatcatg tcccattctg cgtctgtttc gatttgttct
    22141 aactcgcctt ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa ttctacatct
    22201 tcatcttctg aaacaccagc aggatataaa agaacatagt tacgtccaaa ttcttcttgt
    22261 ccgtcaactg ttagtaggat ttcatataat gtttcatttc cttggtcatc caccaatgtg
    22321 atgtgttcgt gtccttcatg gtcgtgggaa tggttatgtt ctgtcataag attacctctt
    22381 ttcttttatt attaaaataa ttgcttacct gagccgtcta aataattttg taagatcatc
    22441 acagctgcta atttatcgat gacttttttt cgtttggctc ttgaagtatt tgcctgctct
    22501 actagcatac gttctgcctg aacagtggtt aatcgttcat cttgataatc aactggcaag
    22561 gagaatagtt ctttgatctt ttctccgtat gacatggaag cttctgccct tggtccgatg
    22621 gaattgttca tatttttagg taatccaact acaaaccggt ctacttcata ttgtgctact
    22681 aattccttca accgatcaaa gccaaactct tcttctgctt cattgatccg aatgatctct
    22741 actccttgag ctgtccagcc aaaggggtca ctgatagcaa cacctactgt cttagaaccg
    22801 acatccaagc ccattactct cataaattca ctccatgatt tgagaggtag tccttgacaa
    22861 ttacttccat gatctcatcg cgttcgtgac ggcgaatcaa atttcgagca tcacgataac
    22921 gaggaatgta ggacgggtct ccagaaagca agtagccgac aatctgattg attggattgt
    22981 agcctttttc ttccaaagcc cgataaacaa tcgctaatgt ttcgctcact tcttttttgc
    23041 gactatcgtc aaaaccaaaa cgtacggttt catctgtaaa acccatgatt ctacacctct
    23101 ttcccgcaag gtaattccag cttaccttct acaaccttat tttactcgaa gaaagggaaa
    23161 actacaaaca aatattccaa acttccgaat aaattaagaa acactatttg tatgtttttt
    23221 tccttttttc tgtagcttta tcaaaagtac tcttttcata aagtatacgc tatatttctt
    23281 tgaatgtatg tttttacaca attttttcat aatattcata aaaaattcga atagctgcta
    23341 ctaatttttt gtttttgttg cttgtcctgt ctcataattg atcgtgtagc catcttgaac
    23401 attgtagata aagacattga attcaatttt tttgtcttca atgctttctg cttccaattg
    23461 cactcccctg gctaccagtt cattcccctc aaaatgagga gtgacacggt agcgtacatg
    23521 atgatttgtt tctttgatat atgccgcaat atcattttcg tgagcaagca tgtatggagc
    23581 attcaacgaa cgtgtccctg tcatcaagtt tttaatgttg ttattttgac cagtcagctg
    23641 gaaaccaatt aaatggctgc gattatataa ccattgcccg tcacttaatt tcttttgttt
    23701 ccaacctgtc gggtcaatat acaaaggctc tctcttttca gtagggaaca aatcttttcc
    23761 aagcatagca ttggcttgtc ctactctgtt caatttatca atatttgaat agctttccca
    23821 tgttcctttg gaaaggctaa gatcctcttt tgtaaattcc ggctgattcc cattgatttc
    23881 tatttcttga tgttttccat catattctgg cacatccgct acttgagaag cttgtggtgt
    23941 ggaatcaaaa atagttgtaa tctgctgaaa gatatctgaa tctttttgtg tggtggaaac
    24001 accgaatgcc cctgcaatca cagcagcgac gatccccacg atcgccttgg ttgttttttt
    24061 gctcatttct ttactccttt actaacagct ccctaaatct taacatatgt tcccctataa
    24121 ataaatacta gaaagaaata gaaacagcaa aaaataaaac ctgttttaag atggcttaaa
    24181 aatacaaaaa agagacggat tcctcgtctc taatcagtca ccactctatc tgtaaggtat
    24241 ttattattat tcaaaaattt tcttcatact caagacaata cactgatttc acaaccgctt
    24301 ggtaaacggt gcgcaaaagc ttgcatctgc ggtaataagt ccatccaagt taaaaacata
    24361 agaagttgtt tttgcttggc tgttcttatt acttgttgca gagcagcttt ttcacaacct
    24421 attttaagcg ggtgacggga atcgaacccg cgacatcagc ttgggaagct gggattttac
    24481 cactaaacta cacccgcaat acaaaaacag tatacgcctc ttttattcct tcctcaagtt
    24541 tttttattct ttacgcttct ggtatgtttt gtagtaaaat aaaactaatc aatcaattat
    24601 tggagaagat gctatgacgg gaaaagaatt agatctgttg ctggacactt gtttactcgc
    24661 tgggaaaatc atgatggaaa gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg
    24721 aattgcttta gcttctggaa atttccgttt ggtcagctat gtcactcaaa ccggtctctt
    24781 tattggtctt gatcgtacct cgaccatccg aatggaacaa attaccaacc gttcgattaa
    24841 tttagaaaaa gtagtaaaag taaataatct ttcacgtaaa tatgttgctt cagagctaac
    24901 attagaagaa ctttactgcg aactgcaagc aatcgaccga gaccgttctt tctttcctgt
    24961 ctggttgcaa atcgttagcg cggcagtgat cagtggaaca attatggtgt tatttggcgg
    25021 aaagatgacc gatttacctg tgactttagt cattggagga ttgggatacg cagcttatct
    25081 atatagtttg aaatttttta gaatcaaatt tttatcggaa tttttatctt ctttattgat
    25141 tggcatagcc gctatctaca gcgtacgttt tggcttagga agcaatcagg atctaatcat
    25201 tatcggatgt gtgatgcctt tagttcctgg agttcaaatt acgaacgcta tccgtgattt
    25261 gctagctgga cattatgtct caggagtttc tcgcggaaca gaagcgatga tgactgctac
    25321 gatgatcggt tttgcgattg cttttatttt ccagcttttt tattaggaaa ggaacacaag
    25381 tatggggacg atatttattc agttttcttt tagtttttta gcgactgcgg catttgccgt
    25441 tattacaaat gtacctaggc gttcattgat ttgttgtggt ctttctggtg cttttggctg
    25501 gatgatctac tggatctgtg ttcaaatagg cggatctgct gcttttggtt cattgcttgg
    25561 ttctttagga gtagcattca tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac
    25621 gattttcaat attcccggga tggttcctct agtccctggt ggtttagctt atcaagcggt
    25681 tcgaaatctc gttactgaat cctatcgaga agcggctcat tatgcggtcc aagcaattat
    25741 gattgctgga gcaatcgctt taggtcttgt attatcagaa gtctttaacc ataacatccg
    25801 taatttcaaa gtaaagcgtg acgcaatcat gatgaaacga aaaaaacggc tttttaaata
    25861 aacgaagtgt aacaaagaaa aggttgtgaa agagacgttc agccttgagc gatgacaaaa
    25921 tatctcacaa acattgcgac aagtaaccgc aggagcaata agaagtaatt atgagtatta
    25981 aaaaaagttt tgtaaaaata gcttctcaca tttttacata aaattgactt aatactgaga
    26041 aactgacttt tgaacaccct ctatctgtaa aaaaggggct gcgacatgat ttttgtcacg
    26101 gcccctcttc tttattcaaa ttttttccaa aacgttcgtt gataataaac gatccataga
    26161 gcggaaagta ttagaactaa accgatcaca ggaatattca ctaaccaaaa tggataaaac
    26221 aaagccaaca ttccgtataa gacgataaga ataatactga tgatcaatga acccatcata
    26281 gttgcgacta aacccaaact tcctgaacct ctgttaaata attggttaat attggtccag
    26341 ttcagcagca gcaaccgata atctctagcg aaataacgta gtgacaataa gtagcagccg
    26401 aataaagaac caaatagcaa gctagggata aaaaacaaag gcagctgaaa agacagtccg
    26461 cttaataatg cgatcccacc agttaaaaaa ctctgcaata tccatccgaa tctgaacttt
    26521 tcttttaaat attggttcat agagattggc aaagaacgga taaacagaaa attttcttga
    26581 tccaaagaaa taagattgct gataaaagaa gtctgattaa ctgttaagaa agccaatgcg
    26641 atccctacca ggaaaacaac accgattaac ttattatcta aatcagttaa ttttatttcg
    26701 cctcctaggg caaaagcaag aataaaaatg attggagtta acaaggaaga agaaagaact
    26761 tgcataatca aattaggttc ttttactaat tgagcattgt aactaaataa aagctgatgc
    26821 acattttggt tctttttatg ggttcttttt attttgaact cactgtttga atcagtcagt
    26881 tgctctgtaa gctttggtac tatcagctgt tttacagcaa ggaaaaatcc cagagccgtg
    26941 atagctagta ttccccaacc caataaacct tgttgagaaa gtggctgact ggcagcatca
    27001 tagaatggca ataatatcga aatcggatgc cgatctgtta actcattttc aatagtcccc
    27061 gtttgatgat tcatccacat gattccagca aatgcgatca gcatcgagct gccaagcaag
    27121 atactagaaa ctgtttttct atgtttttta aacactgctg ttcgagccaa accaaaaaca
    27181 atcaaactag ccagacaaaa aagaataatc aaaaatagag aaaacaaaag gattgagata
    27241 atgatagtaa gcaaaatccc tagatcagct cgccatccgg ttaaaataaa catgactaga
    27301 agcggaaaaa caaaaggaat gatggtcaaa gcaactacca aaatctttgc tgaaaaaacc
    27361 tgttcttgtc caaaaggcag aggcaaaaat acttgcaggt catttccttc aaaaaagata
    27421 ttataaatga ctgatatact ttgagttaaa cctaacatac caaaaagtgc cacgtaataa
    27481 gtaaagaaac ctggcaattt aacaaagttc atagcaaaca tagcaaatcc gtaaatcagt
    27541 aaaaaaatca gccctgaaaa aagattttga gacaaaagat agatagttaa tcgctttccc
    27601 ttttttcctt tctctcttgc tttattcgtg agctgtggat taacatataa aagattaact
    27661 cgaattaatt ctaataattg tcgcttatac atacggtcca tctcctgtcg cttgccttcc
    27721 agccattttc aaataaatct tttctagcgt ttcatcaggt gagccattca ataattcact
    27781 aactgccccc tgataaatca attcaccttt cttcaaaata gccagtttat cgcataactg
    27841 ttgtgcggta tccaatacat gtgtagaaaa aatgactgtt tttccttttg cggcatgagc
    27901 cttcatcatt tctttcaaat caaatgctgc ttgaggatca agtccttgca aaggttcatc
    27961 caatacccaa atatctggat ctggcagcaa cgtgccgatc aaaatcgttt tttgacgcat
    28021 tccatgtgaa aaacttgcga ttgtttcatt ttgatgacta tacatatcaa aaagcgctgt
    28081 caattctgct aaacgctttt cttttttttg tgtgtctaat tcataggcag ctgaaatcaa
    28141 gtcccaatat tctccagccg tcaattgtaa aaaaatatct ggggaatcag gcacataacc
    28201 gattttttgt ttgatactca atcgattttc cgtcaatttc attccatcta ctaaaatcgt
    28261 tcccgatgat ggttcaataa tactaactaa acttttaatc gtagttgatt ttccggcacc
    28321 attatgtcct aaaaatccaa aaatttcccc ttgtcttatc gagagagaca agtcttttaa
    28381 tgcttgtttt ttcccgtaac ttttacttac attgatgaat tcaatcattt ctgtactcct
    28441 tttgtttgtt attacttttt attgtaccct tcgttattgg tggagtaaat gtcaaaaaga
    28501 gtgcataaca aaaaaggata gaaacgacta ggctgctact tgggttgttc tcttctacag
    28561 gaaaacactc ttctaacagg taagatatga gtctttgttt tctttaaaat gattcatttt
    28621 ctatcttttc ttgcataaaa ttctttagtt tatcaatact tctctttccc atctctagtt
    28681 gttctccatt tgcaaatgtt actatttcgt ttactctgct aatagatgta atgttttcag
    28741 tattaatgat ataagctttt aaatctttac ataaatactc atattcgagt gattttttat
    28801 attttttaat tgtgccgttt acgagaatct cttcatcttt caatactagg cctattttat
    28861 ttttataacc tggcatgctc attagataaa ggatttcatc gtaatggata tagtgatcgc
    28921 tattaccata acggacagtt gtccaccttt ttttcgtagc atgttgaaca ggatagtttt
    28981 ctttttcttt caataagtaa ctttggatgc tttttttatt tttttcataa gtgaattctt
    29041 ttatcaaata accactaggc gtaatctttc gattaataac agttattgct ttattcggat
    29101 gagacgttag ataaacaatt ttacactttt cattcttttc tctaagcatt tcgcctagtt
    29161 cgataccgct ataataggcg ttcaaatcga tgtctaggaa aaaaatatct gaggaataga
    29221 tttcattatt tttaatgaat tcaacaaatt gtataacgtt caatccctga acgatctcaa
    29281 atttccaagg acttaataaa attctggatt cttccaaaat ttctactagc attttttcaa
    29341 aaaccaaact atcatccaaa ataaatatac gcgttaccat aaaaagtacc tccactcgat
    29401 attttactat caaaatcatt tcatacaact tttcaacata catgttagga ataaattgta
    29461 attctcttca caatcttttc caacaccttt gaaaccgata tcaaatactt tcaggatgct
    29521 gcttctaaaa taaatatcaa acaaatgaat acatcatgca aaaaaagcca gacaaccgac
    29581 gtccttgcgt gtcagctgtc tgagctatgt ttatttattt tgttctttta aaaagaacga
    29641 tacacatact gatttttcgg agcagagact ttctcagcgt ttgtaacaac tacagatggg
    29701 atttcacgct tgaatggttg ataatagtct gattggatgg ttccttctat tttataccat
    29761 tcatcatttt taaccgttac tccttccggc atatgcgtca acaaaccaaa gactcctgaa
    29821 tctgccacac aatggataat gccgaatcgg aacaagaaga tatctgtatt gctatcgttc
    29881 ggtgtttggt aaacaaatcc ttcatagctg atttttttgc caatgaattc gctagggtaa
    29941 ttataaatta gctccataac ttctaaatag ttttcatctg tgatcttgat agtttgctta
    30001 tccccatact tttctttcaa ctcattcatt tggtctaagt agtctgattt gttgaaataa
    30061 atactggtat ccggctttaa gtattgtgtc tgcatctccg gatcccctac tgattcttta
    30121 cttactggga aattaaatcc tttggcttct acgatcgtag tgtcgagact aactgttgga
    30181 aacaacgtcc ccacgattac tggcaaagct agcagtacat aagcgattcc acgttgataa
    30241 ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt
    30301 ttgacccaca aatacagctg aactaaagcc aaaagagcag ataaaatcat tgaaagaata
    30361 gctaagtagc gataatggac attgatatat tgatcgagtt tgccagaaac ttgcaagtac
    30421 atcatcaatt cgaaataccc agataaaatc aaaaaccgca gcatattttc cctcctaaat
    30481 aaataatgtg aatccaataa cgctaagggc aatcagtccg atcagcgata aaataaagcg
    30541 tcctgtaaaa tatcgtttca tcatcagtag atttttgata tcgaccattg ggccaaagac
    30601 caaaaaggca gctacaggtg ctgtgccgaa cagacttaac aaagaagatc ctataaacgc
    30661 atctgcttct gagcaaagag acatcgtaaa ggccaataac agcatgatga taatggctaa
    30721 aatctttgtt gagccgagcg ttagcataat tctagttggt aaataagtct gcatcgcagc
    30781 tgcgattaat ccgccaatga tcaaataacg acctgtatca aaaaattcgt cgattgaatg
    30841 tgtcaacaca tgcccagttt gatggatcag ctgatttttt ggttcattct tctcactttg
    30901 ttcttcttca tggacatgat gatggtgatt ttctgctact ttagacgtga ttttttcttt
    30961 caatacagcg tctttttgaa aataggcaag ccagacacca atcacaaggg caacaaaaaa
    31021 gctgcctatc acccgccagc ttgccatctg ccaagaattg ccaaatgcga taaaagtgga
    31081 aaaaatcacg attggattga tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac
    31141 tggtacgcct tttttaacaa attgatgaac gatcggtacg atgccgcatt cacatgaagg
    31201 aaagaaaaat cctaagatcg aaccagtaaa aatagagaag atacgatttt caggaagcca
    31261 tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa
    31321 taatacgaaa ggtaatgctt caataacgat cgataagaaa atcgtactca tctgcaaaac
    31381 cgaatttgga ataaaagaaa acatgagttt cctccttttt atttctagtt cattcgcttt
    31441 gcaaatatac gcttttattc aaaaaaaaca agttgctctg taaattttta cacaactctt
    31501 attttttacc aaagcacttc aattgacttt ttctgttatt cccattaaat taaggtctca
    31561 tacatatcac ttctatcata aaagggagaa taaaaatgaa acaaaccact aacttgactg
    31621 aagttcaaga catactgcaa cagtcatccg ttgccgtcat ttatctttcg atgcctaatt
    31681 gcagtgtttg tcacgctgtt cgtccacgac tagaggaact tctcacacat tatgatattc
    31741 cggcacttca tttggatgca tttgaaacac cagaagtagc tagtaaattt gaagtgctga
    31801 ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa gcacggttca
    31861 tcgactttaa aaaaatccgt tatctactag atgaactgac agcagaagat gattcccttt
    31921 cttatgaaga aatcttccga acaaaataat cgttgtccaa acagaaaaaa gcgattgaac
    31981 aagtgaaagt tttgccttca tttgttcaat cgctttttat ttttattctg ctgtattttc
    32041 ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa
    32101 atcaacattt tggatcaata catctgacgg agcattcaat cgagcagcgg taaagttcaa
    32161 gattcctttg attcctgctt ccgttaactg attgactact tcttgtgctc tttcagcggg
    32221 tatcgttaaa attgctactt cgatttgttg gatcttgatt tgttctgaca tatcttccat
    32281 tggataaacg gggataccgt ctacgatgcg cccaacgatt tcctcattca catcaaaagc
    32341 acaactgata cgtatgctat tactttgatg gaatttgtat tttagtaaag cacttcccaa
    32401 gttccctaca ccgatcagcg caacatttgt caattgatcg tcgtttaatg tctttgcaaa
    32461 aaagtgcata agatcttcta catcataacc atacccacgt tttcctaatt cgccaaaata
    32521 agaaaaatct cgacggatcg ttgcactatc tacttgaacg gcttcactta attcagttga
    32581 agacactttt ttctttccag cattatttaa aatccttaaa tagcgataat acaaaggcag
    32641 tcttttagct gtcgcctttg gaataatatg atctttcacg aaatgacctc cacgtattgt
    32701 ctaattacat ctttgatttc tccatcatag cagtatttat aggaaaaaat caattccatt
    32761 gctcagatat tgtgaaattt tattctttat tcatttattt atcatttctg caaatctcgc
    32821 agtataatgg aatttatggt agactactaa ttgaaaaata gttttttatt tgaggagaat
    32881 tcacaaagta gaaaagagga ttccatgata ttattacaag ccaatcagat tgctcgccat
    32941 tttggtgcag aagtcttatt tgaaaacata catttagaaa tcagtacagg cgctcgtgtc
    33001 gctttagttg gcagaaatgg cgctggaaaa tcgactttat taaagatcat tgctggaatt
    33061 gaagcacctg atgaaggaaa gatcgctaaa aataaaacag cgactctcgg atacctcgct
    33121 caggacacag gacttgattc caatgaaaca gtttggaacg agatgctgaa agcttttgaa
    33181 caagttagac aaatggaaga gcggatgcgc gagctagaga ttttcattag cgaaggtacg
    33241 ccagacacac ctgcttatag ttcttattta aaagaatacg accggctgca acacgatttt
    33301 tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa
    33361 ttcgattctt cattttatga tcagaaaatc gatactttat caggcggaca gcgcacacgt
    33421 ttagctttag ctcgcatgct tttgcaacac cctgacattt tgattctaga tgaaccaacg
    33481 aaccatttag atatcgagac actttcctgg cttgagaatt atctgcaaag ctatactggt
    33541 gcattattga ttgtttccca tgaccgttat tttttagata aagtagtcaa tgaagtctat
    33601 gaattaagca gaagaaaaat gcgccattac aaaggtaact attcgaaata tctagatttg
    33661 aaagcagaac agttagcaag tgaatggaag gcttatgaaa agcagcaaac agaaatccat
    33721 aaattagaag acttcgttgc tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa
    33781 agccgcagaa agacattaga aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa
    33841 caagcaaact ttctattcag tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa
    33901 gatgccgcta tcggatatga taatgtcacc ttatctgaac caatcaatct agatatccgt
    33961 cgtcaagaag cgattgctct agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa
    34021 tcaatcattc accagatccc ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc
    34081 atcggctatt acgatcaagg tcaagctgat ctgcatacaa acaagacgat cctagcagaa
    34141 ttgtgggatg agcatcctac cactccagaa aaagagatcc gaagtgcttt aggtgctttc
    34201 ttattttctg gcgaagatgt agaaaaacca atccctctgc taagtggcgg tgaaaaagca
    34261 cgggttgctt tagccaagtt agcgatgaac aaagaaaatt ttttgatttt ggatgaacca
    34321 acaaaccatt tagatattga caacaaagaa gtattagaaa atgcattgat cgattatgac
    34381 ggcacactgc tttttgtttc ccatgaccga tatttcatca atcggatcgc gacaaaagtc
    34441 gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag actatgatta ttatttagag
    34501 aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac aagatgagcc agaatctgct
    34561 cctgctccca cgaacaaatc tgattattac caaagcaaag aacagcaaaa gcttatccgt
    34621 agccttcaac gaaaaattac tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc
    34681 gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat
    34741 gagctgaacc aagatttaga agctgctaga caagatcaag aaacaaaatt gacggaatgg
    34801 gaagaactaa gcttggaatt agaagaatat gaaaattaac gaaaaaacca aaacagaaac
    34861 cattcatgct gttttggttt ttcttttttt attttatcaa acaaaaattc catatttacc
    34921 tttttcacca ataagaagac caattactag tcattctcac taaagcttct aaataatagt
    34981 aatcacccca aatacaaggt gtatttaccc cattattttt gggcttatta taaacagact
    35041 gtgttaaaaa gccatcttct tcttccgttg tagcatatct ttctttcaat tgtcgcataa
    35101 ttttccaaac agcagactgg tataagttct tttctgtttt gtctaataga cctgattcta
    35161 ttaattctaa taatccacaa accatgatca ccatactaga tgtatctttt tcttctctgc
    35221 tagaatcatc aaagatcaaa tcccaataac aaatggtatc ttctggcaac cgattcataa
    35281 aataattggc taatattttg cttaaatcaa aatatttttg ttctttcaaa taacgataac
    35341 ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc ccaacaggaa ttatcactat
    35401 aaccttgatg cgtacttcca tatcttggtt ttccatcttt gacatccata taaaatgtat
    35461 gataagttga accatcttct cttactaaat aatctgctgc attttgaata tgagcctgag
    35521 cagctttttt atatttttga tttccagttt cttcactcgc ccaatacaat aatggaagat
    35581 tcatgcaaca atccatgatc attctacctt gttgtgaagg atcactagga tctccccaag
    35641 cttgtataac tcctgccttc tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg
    35701 aagcttcctt catttttcga tttcccgtta ttttataatc tgctacggtt gataaagaat
    35761 acaaaaaacc aagatcgtga tgattagtga taatttgaca gtcaatcctg tggataaaat
    35821 ctatatctgc tttttcagct gtttgttgaa ataaaggatc acccgtcttc tcaaataaca
    35881 accaattaat tcctgtccaa aatccttctg tccaaccaat attgtctctt aatggataac
    35941 gtccttttat aggacactcg cttgggaatt gatactgact gttactgata cgtttgatta
    36001 acctctccgt gtgtctgcga caacgctcga tctcattatt ccattctttt tccattaatt
    36061 ctttataaca ttcatttatc catttttctg gatgtattat ctcttcaaac atcactttat
    36121 caagcatttc cccaccagcc tttagcataa ttttagtctt tttttattgt ttttattttg
    36181 gatagttagc ccggttgtcc catcaaaatc aattttggga cgattgttcc atacttctaa
    36241 actataatcc cgatcttgat gagcaaccac tcctgttccg agccaatggg ttccttttcc
    36301 aaatgtaccc tctaatccag gaataaaagt gttttcccat gtcatcaagt ttgtattggg
    36361 caaacccata atagctgtgg attttcgttt aatagtatcg tcacttaaac agataatacc
    36421 tgaaaagcca gcttcactca ccctatagtt ttccttgtct tcttctatga ttattccttc
    36481 tttctcagga gaacattcaa tagcaaaccc tgtctcatat agaacatatt ctgtatctaa
    36541 ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga ataagccaag ttctgatttt
    36601 tgcctgtaaa gtaggttccc atgtagaggc aagtacctct ttcttacata caatgtctcg
    36661 tacattattt cgaacgatga actgtccata ggttttgatg ctctcattct taaaactggt
    36721 cgtgatatat gtgtccgctc gctgaatccc tagcattgaa tccattgcca tttctgcttt
    36781 caattgattc gctcgcggta tcgaaaaacc aaattgacta gaataagcaa attttaaata
    36841 tttttcattg gtcaatgtat ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt
    36901 atgtccatta tcatgttgta tgagcaaatt ggcttctgaa agaagtttag ttccttcacg
    36961 aataggcata ggcatttctt tagccgtcca atactcatgg tcttcaggta gcgccaacaa
    37021 taaaaaaatc ttattcagcc ataatggaga taacgtacta ttatagggtt cagtcaatgt
    37081 caattgaggg taggtataac caatattcag gatacctttt tcatcaaata tttctttttg
    37141 catccaccat cttatattcc gattgactaa ccccttgatt tcacctaatt taaaaggcaa
    37201 aagatcggcc cacaacatag ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct
    37261 tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa
    37321 atcaatagct cggttcttat atatctggct aatgggcttt tctggttcta gtacgctata
    37381 aatcaaacca taataatgaa atgcaaaagg attataataa tcttggcgac ctcttgaact
    37441 gtctcgatac cagccatttt gaagataaca attttctata ttatcaaatg cttctttcag
    37501 tgctgattct ttaactggat aaccaaattt ttggataatt gttccgacaa ttatcttaaa
    37561 aaattgccaa ttaccatctg cacattcata ttcaaaaatt tgcgagaacc aatgaataat
    37621 ctggcttttt tctcctgtat ctaatttttt ccataataat tgttcatgat gatataacgc
    37681 taataacatt gcgggcattt ctaccatacg ctggtctctt tcatgtattt ctccccaata
    37741 atcgggatgg gaaggatctg ttccagcttt tattcctgat agaattaatt tcaaccatcg
    37801 ttcatctaat tggtcaattg cgggccccgc tccccataaa acccgagaga atccttccat
    37861 tcctgcaatt ttatttccat atcctacccc ttgatgtata taatcaattt gtgcgccact
    37921 tgctcgataa aacggctgga gtggttgatt tatcttttta aataagtcaa agtaatcctg
    37981 tctggtcctt aatagtggta ctggatgttt cattgtattt tctcctcttt ttactaccaa
    38041 atagctaatt ccgactgttc taatcgaagt aagccttcaa taaaaaaata atcagaatag
    38101 atcagtggca cattataagt cagttctcta tggtatgctt ctgctccgcc tccaatgatc
    38161 ccatcttcat ccatattcca atttgcatag tgcttttctg tcttttggag tatattgata
    38221 gcagcttcat gatacatttt tgatcgtaca gaggaaacat atcctgctaa ttccagaaga
    38281 ccgcatgcag cgcatactcc agcactggta tcaatattcg aatcttttac agcaaaatca
    38341 atgagaggaa tataaccagt tttttcgatt ttctgaataa aatttactgc tatcttttct
    38401 gctatagcta gatagtacgg attttttgtg taacgataac ttaacgcgaa tccatataaa
    38461 gcccatgcat ttcctctgct ccaacaagaa ttctcattgt atccttgccc gccaagtaac
    38521 cgaatatatt tccctgattc tgcatcaaat tctgcgatat gcccggttga tccatctttc
    38581 ctcactagat acttagctgt tgtatctgca tgctgccgag caattttaga gaacctcgga
    38641 tcaccagttt ccctcgaagc ccaatacagt aatggtaaat tcatcatact atcaataatc
    38701 atccaaccag gtttatggtc attccatgct actaaatagt tccctgaaat attatagcgt
    38761 ccagcaagta ccgtagctgc atgtaaccct agttgtcgac tatgtttgtt ttgagactgt
    38821 tgataatgtg caatagccga tggtaagaat aaaaaaccta catcatgatg aacgtctgaa
    38881 aaatggtaca gggcagattc taataattgt tcttgttgta ttgctgcctc tttgaacttt
    38941 tctcgttttg tcttgtgaaa gagctgccat aaaataccac tccaaaaacc attggtccac
    39001 cagtcgattc ctctgtcctt tattacgtct gtatattttc cgttgtaaga aatatatgga
    39061 attttattcc ccattctgtc taattccgca tctatctttt cagaagctac attcaaaatt
    39121 tttcttgacc attccttcgt ctgttcattc atggatatcc cgccattctt ttaattttta
    39181 tgaatgtgga gttgctccta gaacgaacta gatggttaac attcgttatt ttttttactt
    39241 atttgcagct ccaatgtcat tctccttaaa tttagactgc gcccacacat aaatcaagac
    39301 cattgggatc actgaagttg ctaaaattag cataattgcg ccataattaa ttggctcacc
    39361 tgtactagaa accgtcatat ttttgataat taagggaacc gtatatttat ctttatctga
    39421 aagcatgact aatggaaata gatattgatt ccaacttccc ataaagataa gtaaaccact
    39481 agtaataatc gtaggtgata aaatcggtac agcgattttc gtaaaaatca cccactcatt
    39541 ggcaccatcg attctggcag cctccatcag tgctgtagga aattgactgc tatattgtcg
    39601 catcataaac actgaagttg tactggcaat cgaagggatg atcacacctg cataagtatt
    39661 cgataatcct aaacgactca taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac
    39721 catcctagat agcataactg tattgaacaa cagtttattc cccataaaac gatactttgc
    39781 taatgcataa cccgccattg tagaactggc tgtagctaaa actgtactga ttacagcaat
    39841 aacaatcgaa ttccaaataa tcaagggata attatatctc gccatgatgg cttgccagtt
    39901 ttttaatagc atatccccaa acttgaaatt gattccgcca cttaaaattt cttgattact
    39961 ttgcgttgaa gctacaatca tatataaata tggcatcaaa aatattaagg caccaaacac
    40021 aataatgacg tacgtaaaaa ttctgctttg tatcttttgt ttagtcattt ttatcacctc
    40081 cgaatttaaa ttgaatagcc gataatataa cagatacaaa caccatcgtc caggcaatag
    40141 cagaggcttt tccaaaatca accatttcaa aacttgtttt atataaattg actcctacga
    40201 ccaaagctga attttctggt ccattggaag tcccaaagaa tatatttggg atattgaaat
    40261 tacccaagcc attgatagtg gctaaaactg tggccattaa aatgattggc tttagcatgg
    40321 ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc atctatttca gcactttcgt
    40381 atatctcttt agaaatccct tgaagaccag ctaaaaataa aatagtgtta tatcctacac
    40441 taagccaaat catcgtgatg atcattgata ttcgtatcca aaaaggattt gttaaccact
    40501 gtacaccgtt aattcctagc gcattcaaaa atccattgaa taacccagtc ggattgaaaa
    40561 aagtcatgaa tacggaagct acagccaccg ttgatgtaat ggttggaata taatagacag
    40621 ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa taaaacagca aagtaaatag
    40681 ctaatagcat cgttattgga attgatcctg ccatgataat caaaacattc gtgtaagatt
    40741 taataaacaa tggatctcgg aagatagcta gataattgtc aaatccaaca aattcaaatg
    40801 agtagccaat tttcataaaa ctaaaagcaa acgagataat gatgggaatc aaaatcgtca
    40861 caataaagaa aataacaaaa ggtgtgacca atccataagc acttttttta tgagacgtat
    40921 ctgttgcttt tttcttgctg ttctttattg tcttgcttgg taacgaagat tgcatcatcg
    40981 ttaagtcctc ctaatatttt ttaatcacca gttaatttta cgccgtactt atgagcaaaa
    41041 ttttctgctt cttttagata tgaattttca aaatcacctg tttgccaata gttatatgca
    41101 gcggtagtca aataagacgt agcatctgaa taagtggctg gtaaaacagt atttgatgta
    41161 ttttcattga ttttattcaa tagttcatag tatttttgtc ctccttagta gtcaaacttt
    41221 ttctcagctg cttctgtttg ataggccaat gagttagatg tagccacgcc cagctccagt
    41281 gcgttggcta agcattcttc atctgtcagc ataaatgtaa gaaattgttg tgccacttgt
    41341 ggattttttg catctttagg gacataaaaa cttgatcctc ctgatactgc accttttcct
    41401 ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc atttgtcaga tgcatcagga
    41461 aaattcaaag acatattcgt agcataccag ccgccagcaa caaacattgc agattcttga
    41521 aatgctttct cagcatcttg tgcaccgtag aaatgtacaa tatctgagtc gatcatttct
    41581 ttgattaact ttgcagcatg tttcgcttct ttagtattca gacggatatt tccttcttca
    41641 tcaagcaaag attgattctg ctgggctagc atgttgataa acaattcttg gtccccagat
    41701 tctggcaagg caattccaaa actaccagtt ttttctttta atgcttttgc cacctggata
    41761 tattcttccc aagaactgat ttcttcaaaa tctacaccta tttcagcaaa tttttttggt
    41821 tgataataac aaagtgctgg ggtaaaatca ctagcccaag gaataatcgt ttcattactc
    41881 gtttgttgac gcaaaatatt caatttttga tcataaaact gatcgccata tttgtcataa
    41941 aatccatatt catcagcact ataaaaagca tcttgaaatt tttccagcac acctgctgct
    42001 gaagtgtctg ccatggagat aatatccggc atatcttcgt gtgttaccaa ttttggggtg
    42061 atcatttgta ctgttgctgt tcccgaagct acattcacaa tttttaagtc aatatttgct
    42121 ccagttttgc ggttatagat ttttgcagca ttttcaacat agtttgtcag tttgccgttt
    42181 gcgacatata cttctaatgt agtggctttt tttatcattg gatctgcgat gtttgctgtt
    42241 acttcttcgt tagtttttcc acatgcactt aagctgaata atagggtaat gtttagcaaa
    42301 gctatcacca atttgtatct ttttttcatg tctatccctt ctttcttttc aagttcttat
    42361 ataaaacgct ttcacttatg tata
//
LOCUS       DAZUMI010000011        40034 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000011 DAZUMI010000000
VERSION     DAZUMI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.011"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACG81C_000466"
     CDS             complement(51..632)
                     /locus_tag="ACG81C_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5063655.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACG81C_000467"
     CDS             complement(638..1897)
                     /locus_tag="ACG81C_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5063656.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACG81C_000468"
     CDS             complement(1899..2945)
                     /locus_tag="ACG81C_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063657.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACG81C_000469"
     CDS             complement(3180..3455)
                     /locus_tag="ACG81C_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5063658.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG81C_000470"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG81C_000470"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5063659.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG81C_000471"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG81C_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5063660.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG81C_000472"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG81C_000472"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5063661.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACG81C_000473"
     CDS             complement(7283..7918)
                     /locus_tag="ACG81C_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5063662.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACG81C_000474"
     CDS             complement(7984..9387)
                     /locus_tag="ACG81C_000474"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5063663.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACG81C_000475"
     CDS             complement(9374..10405)
                     /locus_tag="ACG81C_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063664.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="ACG81C_000476"
     CDS             10436..10657
                     /locus_tag="ACG81C_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5063665.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="ACG81C_000477"
     CDS             complement(10822..11559)
                     /locus_tag="ACG81C_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS5063666.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11712..13052)
                     /locus_tag="ACG81C_000478"
     CDS             complement(11712..13052)
                     /locus_tag="ACG81C_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5063667.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13289..14812)
                     /locus_tag="ACG81C_000479"
     CDS             complement(13289..14812)
                     /locus_tag="ACG81C_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5063668.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15190..15801)
                     /locus_tag="ACG81C_000480"
     CDS             complement(15190..15801)
                     /locus_tag="ACG81C_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5063669.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15924..16049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16131..16847)
                     /locus_tag="ACG81C_000481"
     CDS             complement(16131..16847)
                     /locus_tag="ACG81C_000481"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5063670.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACG81C_000482"
     CDS             complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACG81C_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5063671.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(17406..18176)
                     /locus_tag="ACG81C_000483"
     CDS             complement(17406..18176)
                     /locus_tag="ACG81C_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5063672.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18173..18544)
                     /locus_tag="ACG81C_000484"
     CDS             complement(18173..18544)
                     /locus_tag="ACG81C_000484"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063673.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACG81C_000485"
     CDS             complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACG81C_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5063674.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19546..20019)
                     /locus_tag="ACG81C_000486"
     CDS             complement(19546..20019)
                     /locus_tag="ACG81C_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5063675.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(20124..20987)
                     /locus_tag="ACG81C_000487"
     CDS             complement(20124..20987)
                     /locus_tag="ACG81C_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5063676.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(21183..23282)
                     /locus_tag="ACG81C_000488"
     CDS             complement(21183..23282)
                     /locus_tag="ACG81C_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5063677.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(23502..24611)
                     /gene="rpoD"
                     /locus_tag="ACG81C_000489"
     CDS             complement(23502..24611)
                     /gene="rpoD"
                     /locus_tag="ACG81C_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5063678.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(24635..26476)
                     /gene="dnaG"
                     /locus_tag="ACG81C_000490"
     CDS             complement(24635..26476)
                     /gene="dnaG"
                     /locus_tag="ACG81C_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5063679.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(26658..28973)
                     /locus_tag="ACG81C_000491"
     CDS             complement(26658..28973)
                     /locus_tag="ACG81C_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5063680.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(29158..30018)
                     /locus_tag="ACG81C_000492"
     CDS             complement(29158..30018)
                     /locus_tag="ACG81C_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5063681.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(30077..30709)
                     /locus_tag="ACG81C_000493"
                     /pseudo
     CDS             complement(30077..30709)
                     /locus_tag="ACG81C_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            30876..31637
                     /locus_tag="ACG81C_000494"
     CDS             30876..31637
                     /locus_tag="ACG81C_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5063682.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31663..34254
                     /gene="mprF"
                     /locus_tag="ACG81C_000495"
     CDS             31663..34254
                     /gene="mprF"
                     /locus_tag="ACG81C_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5063683.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(34442..35629)
                     /gene="tuf"
                     /locus_tag="ACG81C_000496"
     CDS             complement(34442..35629)
                     /gene="tuf"
                     /locus_tag="ACG81C_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5063684.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            35997..36353
                     /locus_tag="ACG81C_000497"
     CDS             35997..36353
                     /locus_tag="ACG81C_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5063685.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(36594..37598)
                     /locus_tag="ACG81C_000498"
     CDS             complement(36594..37598)
                     /locus_tag="ACG81C_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5063686.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            37764..38615
                     /locus_tag="ACG81C_000499"
     CDS             37764..38615
                     /locus_tag="ACG81C_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5063687.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            38632..38877
                     /locus_tag="ACG81C_000500"
     CDS             38632..38877
                     /locus_tag="ACG81C_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063688.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            39064..39768
                     /locus_tag="ACG81C_000501"
     CDS             39064..39768
                     /locus_tag="ACG81C_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063689.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        11302 a         8403 c         6864 g        13465 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    11641 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    11701 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    11761 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    11821 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    11881 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    11941 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    12001 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    12061 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    12121 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    12181 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    12241 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    12301 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    12361 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    12421 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    12481 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    12541 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    12601 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    12661 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    12721 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    12781 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    12841 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    12901 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    12961 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    13021 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    13081 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    13141 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    13201 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    13261 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    13321 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    13381 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    13441 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    13501 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    13561 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    13621 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    13681 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    13741 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    13801 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    13861 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    13921 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    13981 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    14041 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    14101 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    14161 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    14221 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    14281 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    14341 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    14401 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    14461 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    14521 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    14581 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    14641 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    14701 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    14761 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    14821 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    14881 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    14941 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    15001 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    15061 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    15121 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    15181 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    15241 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    15301 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    15361 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    15421 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    15481 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    15541 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    15601 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    15661 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    15721 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    15781 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    15841 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    15901 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    15961 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    16021 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    16081 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    16141 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    16201 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    16261 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    16321 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    16381 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    16441 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    16501 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    16561 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    16621 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    16681 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    16741 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    16801 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    16861 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    16921 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    16981 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    17041 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    17101 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    17161 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    17221 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    17281 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    17341 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    17401 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    17461 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    17521 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    17581 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    17641 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    17701 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    17761 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    17821 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    17881 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca
    17941 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    18001 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    18061 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    18121 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    18181 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    18241 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    18301 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    18361 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    18421 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    18481 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    18541 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    18601 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    18661 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    18721 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    18781 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    18841 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    18901 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    18961 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    19021 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    19081 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    19141 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    19201 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    19261 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    19321 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    19381 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    19441 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    19501 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    19561 tcttttttct ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt
    19621 tactaaaaaa tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc
    19681 tacttcctcg atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc
    19741 ttcttttctc aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt
    19801 caattctgtc aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt
    19861 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    19921 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    19981 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    20041 taataattac aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt
    20101 taagaaaaac atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt
    20161 tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg
    20221 ctgataccaa aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc
    20281 tgatctgcag aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca
    20341 tatgctcttg gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga
    20401 ccttttatct gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca
    20461 agatagaaat cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct
    20521 atcacatctt tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt
    20581 tcatctgcta aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag
    20641 cgatcccctt ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca
    20701 aagtctttat ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta
    20761 acgattccaa aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt
    20821 tcatgttttt gattttggta accgaatcct tcaactgctt caccgatttt gtgttcacct
    20881 tctgatttgc ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt
    20941 tcatcaataa taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc
    21001 tatagtgaaa ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg
    21061 aatagctgag ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac
    21121 tccccttttt cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa
    21181 aattaattgc tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa
    21241 aatggcaaag cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat
    21301 agattataat agccttcccc attttcgatt ctggcttttt ctaatctgta acctccttca
    21361 acgatatatc cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac
    21421 gtccatgctg gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga
    21481 ttcgctgagt ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg
    21541 accgcagctt gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt
    21601 tcttcagctg cttttttcgc ttcttctgct tttttctttt cctcttcttc ttttttctct
    21661 ttatcagtga gcatcttatc tacttgtttc aattgttcat tcaactgagc agatagatct
    21721 gtatttttca ctttttctac tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa
    21781 tccactactt ttccgtcttt aaagactata gctgtttttt ctttagctgt ttgcaattca
    21841 gcatattgat ttttagcttc tgtttgtgct tgttgaatca attttccaaa atctgtgtta
    21901 tccgtttcga ttgcttggat ctctttatct gctttcaact tgatgtcatc tgctagatga
    21961 tcatcgacga tggcaggtgc tatgaaccac tcattcactt tttggatatc tcggatctta
    22021 gattggatat cttggtaagt tgtttctaac tctgaatatc ctttttcatt cttaagactg
    22081 gataaatcat tttttagttt tgataaatct tgatgaatca tatctgcacg aataaattga
    22141 tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt
    22201 ctttttgctt ctgctgctac ttcggcttct actttttgat gatggtcata ataagcccag
    22261 cttcctccac cgagtagaag tactgctgct aaaacgagat aaggtacttt ttttgatttt
    22321 ttcttttgcg ctttatctgg atctgttttt tctaagtttg attcttgatg tgttttttca
    22381 gtatctggca cgattgttat tgatttcgct tctgcttcaa acagcttagg ctggttctct
    22441 tctttttcta tttttgcttc aagatcagta ccctctttgc ttacagattg attttcagca
    22501 tcggattcac tatttatttc atcgggctct gttgactttt tgtctgtgtt ttcgagtatt
    22561 tcttttttct ctgcttccgg cagtttattc gttttttcct tttctaattg tttttcagta
    22621 aatacttcag ttttttctgt gatatccgtt tttacgtctt ttatttttac tgtttcttta
    22681 cctgtggcgt tttctctatc agagtctttt tcagtttctt gttccttttc tggctctact
    22741 ttgttttcct gtgcaatttt ttcactgatc attttgagca gctctttcgc gtgctcttct
    22801 tttgtatctt cacgatgtgc tttgatataa gcggatagta tcgaattttc ttcctcatta
    22861 tcgacgattt ctttcagttc agctactctt cttttttgtt ttgattcaag cgtttcttct
    22921 tgattctttc ctgattcttc cgaagtctca tgaacagttg ctttcttctg ttcatttttc
    22981 tctatattag ttacttgatc gtgagcaggt ggttcttcgt cttctggttc tgctttttcg
    23041 aataattcca ttacagattc tagaggtaaa tctttcaatt ccgaccattc gattggatcg
    23101 tttaattctg tatcaggaaa gactgcatct tcagttgatt tatcctgcat agctgcatct
    23161 gtatttgttt cttcaattga ttcttttatt tgctgatctt ctgcagttat ttgatgaccg
    23221 cagtctggac agatagtatt ctgctccatt tttttcgatc cgcaatttgg acattttttc
    23281 atcacttagc gatcggctcc tttactaggt ttacgctacc atttaatcta tcatattcta
    23341 tacgtcacgc caatagctta tttttaggat ttacttaaaa ataatgaatg aatttccttc
    23401 ctatagaaaa aaaagcttga gactgcgtag tctcaagctt tttttctttt aaaataaacc
    23461 aaacagtttt actgatttct cttctggtca atagtccacg cttattctaa gaaatctttt
    23521 aattgttttg aacgagaagg atggcgcaat tttcttaatg ctttagcctc gatctgacga
    23581 atacgttccc gagtaacacc aaatactttc cctacttctt ccaatgttct cgtccggcca
    23641 tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt
    23701 acatcttcca attgttcttt tagtaactca taagctgcgt gttctgctgg acttgttgct
    23761 tcctggtctt ctatgaaatc acctaagtga gagtcatctt cttcaccgat tggcgtttca
    23821 agggaaacag gttcttgcgc gattttcaag atttctcgga ctttttctgt tggaagatcc
    23881 atttccgcac cgatttcttc tggcgtaggt tctcttccaa ggtcttgcag caattgacgt
    23941 tggatacgta tcaatttatt aattgtttca accatatgaa ctggaatacg gatcgtacgt
    24001 gcttggtcag cgattgctcg agtgatggct tgacgaatcc accatgtagc ataggttgaa
    24061 aacttaaatc ctttgcggta gtcaaatttt tcaactgcct tcatcagtcc catgtttcct
    24121 tcttggatca agtcaaggaa ctgcatgcct cgtcctacat aacgtttagc gatacttact
    24181 actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt
    24241 ttcaatgcta acgctacttc ttcttccgca gttaaaaggg agacacggcc aatttctttc
    24301 agatacatac gaaccgggtc attgattttt acaccggttg gcgcagaaag atcttctgtt
    24361 tttgcttgtt cagctttttt ctcagcactc ttcaagctgt gaattgatgg atcaccattt
    24421 tcatccacca cactgatacc tgcgtcttca atcttttgga tcaatttgtc catttcatct
    24481 gcattcaaag tgaatggtgt agcaagctga ttggataaat catcatatac aacttgtcgt
    24541 ttgggtttgt tttctttgat gaaagctgct actgctgctt catacgtttt tttatctgtt
    24601 ccatttgcca taaaagaagg ccccccttga gtagctattt ggcttgtttt aactgtttcg
    24661 ttaaattgat gatttcaatc gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg
    24721 cttcttgctg catttgtttt tttaatgtta tttcatcagc taagccagat ttgctgatca
    24781 catctagcaa gtcttggatt tctcgtggcg aactttcttc tgccaccggt aaatattcga
    24841 tttccaccac caaccgcctt aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt
    24901 tgaattcata atgttgtgag acatatgcat cgaataaaaa ataaacttct gcatatgcat
    24961 catgaataaa ctgaaattct cgttctttta aaatattacg gacagaaact tctttaaatg
    25021 cacgatataa tagaatctgc tctgcctttt ctacttgcgt caaaggtctc tttttccgtg
    25081 cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg aggttcttga cgattttgct
    25141 gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt ctctctgccg atctgaaact
    25201 ctgttgacag ttgagataga tacatatctt gttcgattgg tgaaacaact cggctaagtt
    25261 caactagtaa ttcttcgagg tattcgatct tgtctttttc attatctaaa tttttccctt
    25321 gttttaaata acgtgattta aaagtaaaga ctgtttcgcg cccatgagcc actaattctt
    25381 ggaaagatgc ttctccatac ttacggagat attcatcagg atcaagcttt tctggaacga
    25441 ctacgacaga aagtgctaat cgactgtttt gccgaagcaa ttcaatccct cgattggttg
    25501 cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca
    25561 tttgttggat ctgttgagct gttagacttg tccccataga agctaatcca gttttgatac
    25621 cagcctgcca tgcagcaatc acatccataa acccttcgaa caacaaaatt tctccgtttt
    25681 tccggatatc gcttcttgct ttatcaagat taaataaaac ttgtctttta ttgaacagtt
    25741 ctgtttctgg actattcaag tatttgggct gatctttttc ctgccctttt tcaggtgcaa
    25801 gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt acgaagtgga aacatcactc
    25861 tttgataaaa acgatcagac atacggccat catcatgcgt gacaaacaat ccagagcgtt
    25921 ctaacaattc atttgaaatt gattcattct tgaatacttg ctgaagaaaa gaacgctcat
    25981 taggtgcaaa acctatgttg aattcttcaa ttaattctaa agacaaccca cgactttgca
    26041 aatattctaa agctggctgt ccagcttttg tatgcaatag catgtgatga tagatttccg
    26101 ctgctttttc atgagcagca atcagtttcc ccgtagcaga ttcaggattt tggacagcta
    26161 tagtacgctg acgccattga tcttctaacg gtatctgttc gaagtctgcc acttttacca
    26221 ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa agtaaacacg tttccgcctt
    26281 taccacaacc aaagcaatga aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac
    26341 gttcttcatg aaaaggacac agccccaagt aatttttacc agactttttc aactggacat
    26401 attgtccaat cacctcaacg atattcgttt gactgcgtac tgtttcgata acctcttgag
    26461 gaatccgttg agccattttt tcaccccctg ctctattgat atcttttggg tttaacagaa
    26521 gatatttcag gagaataacc ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa
    26581 ggacacaatt atacatgttt gattgtatca tatttcattg ctttttcaag tataacacat
    26641 ttctatctca ttaagcatca atttgctact cttttcagta actgattttg aatatcagcc
    26701 ttccagtaca atactcgaag aacaatattt acgagcatga atacaaagaa gtatcctacg
    26761 ctctctccca accctaagtt tacttccaaa attccatgga acagcattgc agctacatat
    26821 atccctacga tcgtcgttac tgctaggaag atgcgcaatg ggttgaaggg ataacaagct
    26881 tttacaactg ccatgcaact gatcccaatc agcaagtaat acatcaaagt agtagtttca
    26941 gcatttcccc atgcgaatgt ttttccaagt agatacaccg caatgatatt cactactaca
    27001 agcaaggcat ttggtaaagc attaataagt gctgtaggca aataacggta agtgattttt
    27061 cttttatctt gttcaaaaga taaaaagaag gaaggatacc cttcaatcgc taaatcaatc
    27121 agcgtgattt ggatagggat aaacggaaat ccaattgctg taacggcaca tataatcgat
    27181 aaaataaatg aataaatcgt tttgataaaa aagatgcctg agactttcgt cacattgttg
    27241 accactcggc gtccttcaaa caggacttct ggcaatgtag taaaatcaga gtccaacaat
    27301 accagattcg caatctgacg tgttgctcca tcaccttctg ccatagcaat gctgcaatct
    27361 gcttctctta acgccaaaac atcattcaca ccgtcgcctg tcattgcaac cgttctgccg
    27421 ctttctttca attcatttac cagcaatttc ttttgctgcg gactcactcg tccaaaaacc
    27481 gtatagcgat gagcagcttc tcgcacttcc atttcttcag taatttgaga aagatcgaca
    27541 tacgcatcat aattcggtaa tcctgcacgt ctagcgatat tggagacagt aaaaggattg
    27601 tctccagaaa tcactttcag atccacacct tcctcatgaa gataagctag tgtttcttga
    27661 gcgttttttc ggattggatc atcgatttca aacagagcaa ccggctcaac aaatgctggt
    27721 ttttcttcgt tcaattgctg atttggagcg atccccaaca taagtacgcg atagccattc
    27781 gcttgggctg tttttagact gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct
    27841 cctacgtaca cgactcccag ttctggaaat tccatcgcgc cccatttgcg atcagaagaa
    27901 aatgcgactt tgtttgtcac atcataccga ttagaaagct caaaataatc tctcatagcc
    27961 cgcatagtga tgttgttatc actgctttct gccagatacg tccccataat ttctgggatg
    28021 gctttctcgt acagcggatg tagtgcttct acccgctgga tactcatttt cccttcagta
    28081 atggtccctg ttttatccaa gcataatgta tcgacatggg ccagcgtttc aattgaatac
    28141 atgtcttgaa caaggattcg tttttttgcc agtttggtta cccctgtcgt taatgcaata
    28201 cttattagca ataccaaacc ctttggaagc atgcctaaca aagctgctgc agatgcaata
    28261 acagaaattt tgatagaagc atcgcggatg aacagtgctt ccaaaaatag tatgatcccc
    28321 aaaggaataa tcacgaaact agtgaatttg gatactttgc gaatcgaatc tacaagttca
    28381 gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc ggacagcata attatctgca
    28441 cctactcgga taacttcagc catgacagaa ccgcttgtca aaaagcttcc agaaagcaat
    28501 tcagctccag tttctttgac aatcaaatca gattcgcctg tcaaaagagc ttcatttgct
    28561 tctgcccgtc cagatataac ctgtaaatcg gctggtatct gttctcctgc tccgattttt
    28621 accaaatctc ctaataccag ctctttagta gaaatcgatt gttctttacc gtcccggatc
    28681 actagtactt ctgattggga aacaatcgat aatttccgta caagatttct tgcatgtatt
    28741 tcttgataaa ttccaataac gatattgacg acaataattg ctaaatacgc catgttggaa
    28801 tacgcaccta caaataacag acaaatccca ataccaaagt tcagaaaatt gaataacgtc
    28861 aatatattat cgctgaatat ttgttttgta ctttttgatg catcttcctg gtagtcattt
    28921 tgctggccag aagccatttt ctgaactacc tcttgctgtg taagtccatt cattttcttt
    28981 ttctccattt tccgccaact ttcaaaaata atcacgtgtt taagtctagc gtgttccaag
    29041 attaaaaaca acttgttttt ataaaattta tagaaaaaca cacgaatgat tttacaaaaa
    29101 agatgccaac ctttttgttt gaatcacccg tgtgctgttt ttattatcaa tctatcttta
    29161 cttatattgt tcctccaaac ggctcatcca ccaacctagt gctgtaccgg ctataaaaag
    29221 aacaggacaa gctagtaccc cagcaactcc caatcccgcg taatcggtag gaatgatcat
    29281 aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat agcttgctat atgcctttga
    29341 gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa agaatcccac caattccgat
    29401 cggcacaatc acttcaagtc gtacttcttt aaatccgtca gccatcgctt tatacaatcc
    29461 catatacacc aaaaaattag aagggctcaa tcctgggacg atcataccca aaccaatcaa
    29521 tccgccggca atcagccatg ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc
    29581 gccgtataat aaaaacaaat aagccagaac aaaagtaaca accatgatga tcacatcaga
    29641 agtatctctt ccttttttcc ctgcttcttt ccataacgca ggaacagttc cagcgataca
    29701 tccgacaaaa aaccataata tcgttgtttc atacgcacct aataaaaaac tgactaaaaa
    29761 agaaagtaga aagatgcctg tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt
    29821 ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc agccgctcat acatgccaaa
    29881 gacagctgct aatgcaccac cgctgactcc tggcaagata aatcctgaac cgataaacat
    29941 tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac cccacttctt gattttccat
    30001 tttttgcctt ttttccacta tttgcgtact ccttctttca tttaatttta ttcatgattg
    30061 aacatttcct gctattttac ttaaagactt tttcataggt ctctattatt ttttcattca
    30121 ttttgacaat atcaggataa aacatgttac ctccattatg atagagatag ccgccattgt
    30181 aaagcaagga ctggatccgc caatatcggt attgtttccc ttcctcattt cctaaaagtg
    30241 ggctcagcac atctttagaa tacgcttctg ctaattctac tgtgttttct ccgccatgat
    30301 tttttacata ggcaatataa tctttcccaa aattatatgc ttggacagct gtccataaat
    30361 cgcatccttg tttctttgct tctactatca tttccgccaa atatttgaca ccttgatcaa
    30421 tactgtcagc tggattatca atgatctcag cttctccatg cagactttcc gagctctgca
    30481 tgagatctgt cccttttcct tttgattcgg tcaatattat cgccttgatc aaattctcgt
    30541 agttttccat atgataattt tttgcagctg ttgttatttc tatttgatag ctttccactc
    30601 gttttaccga acgataagtt cttcctcata aaaacaacac cgcaccaata accgtcagaa
    30661 tgatcatgta aaaaagaata cggataatag ataatcgttt tcgcttcatt tcatcaccta
    30721 gttattttga catctttttt tatcttacta tttctttatc ttcttggcta tttcttcctt
    30781 gatttctgtg tacgccttaa gaaaaaatga attttcctgt attttttgac atcttacgaa
    30841 aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac tgatataatc gttatacttt
    30901 acacagtagg tgattatgtg aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg
    30961 aaatttacta tgaaaagagc ggtcaaggct tccctctctt tttactgcat ggcaatgacg
    31021 gcagcggccg ctttttttca gaacaggtcc ctgtactcga acgctactat accgtgtatc
    31081 tagtagatag ccgaggacat ggtcgttcga caaacgaagc atctatgttg aattttcagt
    31141 taatggccga ggatctgaat acgatcatgt tgctggaaaa aatcgatcag gcagattttc
    31201 ttggatttag tgatggagca aatctagcac tggtatttgc cagcagcttc cctaaaaaag
    31261 ttcatcgatt gattttaaat tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca
    31321 gagtcatcag taatattcat tatgcatggg tatggttact ctctttattt cgcccaagtc
    31381 ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga catcgggctt acagaaaatg
    31441 atttaaaaaa aatcaactcc cccactttga tcatcgtggg aaaaaaagat gtaatcaagc
    31501 taaaacattc tttgtatata gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag
    31561 aacaaggaca tgaattagct agaaaagatc ctgaacgctt caatcgagaa gttcttcaat
    31621 ttctttctga aacataacta ttaggaatag aaggtgattt atttgttaaa aaataccata
    31681 caatggctga aaaaccacct aggactgttc aaaacaatct ttttgatttc tgtcattgtc
    31741 attattgtcg gtgaattgat gtccatcggt aaaactctat ctatccagca attaggagaa
    31801 acgtttgcca ctatacctgt atggaaaagc ggactaatgc tattgattgg tcttatctct
    31861 gtgttaccta tggttggtta tgatattatt ttgaatcgga tgcttgaaca aaagcagaat
    31921 cctcgttacc tgtttgagac aagctggctg atcaatacga tcaacaatat tgccggtttc
    31981 ggcgggtttg ttagtgtggg gcttcgctct gaactatatg gacaaaagaa agagagcaga
    32041 aacgtgatcc aagcattatc gaaagttttc ttgtttttga tggctggact ttctatttac
    32101 agtctgcttt cttttttact tgtagtcttc acgccagttg ctcctttttt gaagcaatat
    32161 tggatatggt tgatcggcgg cggtctatat tttcctgtcg tctttttatt tactactttc
    32221 aaaaagaaag gattccttgg tggtacctca cttaaaactc aaggacagct attgctagtt
    32281 tcattgttgg aatggaccgg tgtcctgttt agtttccttt ctgttggtta tttaatggaa
    32341 atccggattg atctgtggca aacaattcca ttattcattg ctgcttcagt gataggaatc
    32401 gtgtctatga ttccaggaga aattgggagt ttcgatgtaa tgatgattat cgggttgtcc
    32461 gctattggtg tgccaagaga aatagtcgtt gtgtggatct tgctctatcg cctgttctac
    32521 tacatcattc cgtttttgat tggaatcgtc tttttcttca aaaatatcgg ttccactttt
    32581 gatcagcgtt attctggtat ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg
    32641 gttgtcttat tatacttttc tggaatcatg ctagtacttt ctgccactat cccgcaagct
    32701 tttacagagt ttcgctggct tcatagtttg aacccattga aatttcattt tattatccaa
    32761 tttcctagta tactattggg atttctttta atcattatgg gcagagggat cgcagcacgt
    32821 gtgaaacgag catatttgcc tacaatcttc ttgattgttc tagctttact ttatgtttta
    32881 ttaagtgatt ttagtttcac accggtggtt ttcttgtcta ttctgcttct gatcattctt
    32941 gcttctaaaa acgaattatt tcgggaacaa ttgatctatt catgggagtg gcgtacgatc
    33001 gatggaatca ttattggtgc actgtccttg ctttacatcg ttattggggt atacaatctg
    33061 ccggatttcc ctcatcggcg tcaccatttt atttcctttt tcttatttcc ttctgaaaag
    33121 atttggtttt cgggattgtt ggccatcatc gctgttagtt tcatgatcgt tctatttgtt
    33181 catttccttc aaggagaaaa gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg
    33241 aagatcctta caacttatgg aggaaactcc gatagtcaat tgatcttctt aaaagataag
    33301 cggatgtttg cctatgaaaa agacggtgaa ccaactgttc ttctccaatt cgcttgcttc
    33361 aataataaat gtattgtaat gggtgatcct tctggtaaga aagaagattt tccagaagca
    33421 atcgaagctt ttatcgaaga aacagatcga ctatgctatt taccagtttt ctacgaaaca
    33481 agcgaagaga ttgttatgat tttgcatgaa ttcggctacg atttcattaa gatgggggaa
    33541 gaagcttatg tagacctgaa tagttttaca acttcaggga aaaaaatgaa gggcactcgt
    33601 gctgtcttga atcggatcga acgagaagga tttacatttg atgtcttaca accaccattt
    33661 tctactgagc aaatgagtac tttcaaaaat atctctgata attggttggg ttcacgaaag
    33721 gaaaaagggt tttctttagg ctttttctct gaagattatc tacaaagagc gcctattgca
    33781 gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa cgatcatgcc tacttacacg
    33841 aacaataaag taggaacgat tgatctgatg cgatatgatt ctaaaaaagc accttctggc
    33901 agtatggatt tcttgttctt gcatttgttt gcctatatga aagaagaaca tatccaatgg
    33961 ttcaatttag ggatggcccc tctttcaaat gtcggaactt ccagaaagag ttttttacag
    34021 gaaaggattg catacttggt ttacgagttt ggttctcatt tttattcttt ccatggatta
    34081 agagaatata aaagcaagta cgctacaaac tgggtctcac gttatactct atattctaga
    34141 gatagttgga ttgcatatgt tatgattgct ttgctgatta ttgataatgc gccagttgag
    34201 cagacacaag gtagaataag tggtttgaaa aaatggatac gaagaaagta ttaaaaactg
    34261 cataccgata gatgaacact ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc
    34321 atgcttctac aatctcttct tatacaattg aagaacaaag agcggtccaa acagaaatcc
    34381 tgtttggacc gctcttttat tttaaacatt tttcattacc aaaatattcg agtcgcgttt
    34441 cttatgcttc gatctctgta acgatacctg aacctactgt ccgtccgcct tcacgaattg
    34501 agaatgtcgt tccgttttca acagcgattg gatggatcaa gtcaacatca atcgtcacgt
    34561 tgtcaccagg catgaccatt tctgtatctt ctggcaatgt gattgttcct gtaacatcag
    34621 ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa tggagtatga cgtccacctt
    34681 cttcttttgt caacacgtac acttctgctt tgaattttgt atgtggagta atggaacctg
    34741 gtttagcaag tacttgtcca cgttcgatat cgtcacgttg gatcccccgt aacaacacgc
    34801 ctacgttatc cccagcttcc ccgtaatcta acgttttacg gaacatttct acacctgtta
    34861 caactgcttt ttgtgtttca ggtttgatcc ctacgatctc tacctcatca ccgacacgaa
    34921 cagcaccacg atcaatacga cctgaggcaa cagttcctcg acctgtaatt gagaagacgt
    34981 cttccactgg caatagtaat ggtttatctg tatcacgttc tggtgttggg atatattcat
    35041 ctactgtatc catcaattcc ataatagcag cttcagcatc tggatcgcct tgcaatgctt
    35101 ttaatgcgga acctttgatc acaggagtgt catcgcctgg gaaaccatat tcgctcaata
    35161 actcgcgaac ttccatttct accaaatcga tcaattcttc atcatcgact aaatcaactt
    35221 tgttcaagaa aacaatcagg tattttacac caacttggcg tgacaataaa atgtgttcac
    35281 gtgtttgagg catcggaccg tcagttgcag aaacaactaa gattgcacca tccatttgtg
    35341 ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt
    35401 agtggcgttt ttctgtttca tattctacgt gtgcggtatt gatcgtaatc ccacgttcac
    35461 gttcttctgg agctgcatca atactagcat agtcttgagg atttgctagg ccttttttgc
    35521 ctaatactgt agtgatcgca gcagttaaag tagttttccc atggtcaacg tgtccaatcg
    35581 taccaatgtt aacgtgtggt ttacttctgt cataatgttc ttttgccata atttacaaac
    35641 ctcctaaata atatagatca ctgaaaaatt tcagctctca cctttttatt atagtctttt
    35701 tttgactaaa tcaaaaagaa tacgtccgac ttactttttg taagattcga actaagctta
    35761 ttatacgtca atattggtca aagtcaacta agagcactcg tctgtcttat acgcgaaaaa
    35821 agtacctttt ataaataaaa aaatgtgttt tttgagaaaa acttcaagat agtccttaac
    35881 tttcttttga agcaataaga acaaaatgag gaaaagttta aaattcagct gttgccaaca
    35941 actttcaata gacgaaatct tctatttcgt taataataag gaaagaggtg aagtacatga
    36001 aaaaattaat tggcgtactt gtcgttttag gcatatttct cggcggaagt tttgcagctt
    36061 atcattattt ttacggcggc gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt
    36121 cagctaccca agcagataac ggagagaaat tcacgactta tacctacaag caagcggctt
    36181 ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga agaaagagaa catccattaa
    36241 agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa aggtgtatta agctgggaag
    36301 aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg
    36361 tcttaaaatt ttattagata aaaagaggct gggacagaag cgtttaactc caagaaataa
    36421 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
    36481 tttacttttg tctcacgctc tttttttgtt atattcgtct tttccgatcc ttctcttgac
    36541 acaattcatt taactgatca ctggtacttg aacacctaat attctagtca tttttataaa
    36601 cgattcaata cttcatccac atttttgatc atcacggata tcgtaccatc cggattattc
    36661 acaaattcaa tcaaatctgg gttgcgatat acatctactg gtacgatcag ttcgatcccg
    36721 tttgatagct tgaatttttg ttttccaaat tttttctcag aaatttcttg tacttctttt
    36781 acaggaggtg cttctgggac aaatccttgc tcttgtactt cttctttaaa agccattttt
    36841 gccgtgatgt tttccttgaa gactttttcc gcaatcatct catgatccaa ccgacctttt
    36901 tcttctatcg tatcatagac agcttctttc aaatcagcca tgatatcaaa ttcttctgtc
    36961 tcaaattttt ttccaagatg cttggctact ttttggatca cttttacatt ttcttctaaa
    37021 gaaggtgcag gaacactttc gatcactctt ccagaaaaat aaagctcttt tttgccagaa
    37081 aactcatatt ttttttcaat taattcgtag gtccaatcag tcaaattcac agcgaatgct
    37141 tcatctgctt tttgtgtttt cgcccctaga attgctcgat tcaaaatcaa gcggttgtcg
    37201 atccctgctt cgtctgcttc aacaaagtga gtgtaagcct cacgataatt gactttcaat
    37261 aaagataagt acatcactgt atctaattca tacaagacaa caaatacatc tgcactaggt
    37321 gcttcttcac tttcttggta gatatcgaac caccgctgaa cgattgcttc acttgcttcc
    37381 acaaaatttt cttgagcgat tttaagtgat tcaacgatca tgctttcttc tgctagttgt
    37441 ccagtttttg tttgagcaga agagattttt tggatcttct tttgaagaaa atcacgaata
    37501 tattcttttg tcagatccag ctctttttgt gaaaagactg gagaaccagt ttctctgtca
    37561 ataatatgca gaattgcttt ttttaaatag atatccattt tctcattccc ttctgtctta
    37621 gtttacggaa aaaaaatcaa aaataccaga atcaaaatca ttttctttta aacaaaaatt
    37681 tcaagagtag aaacaaaatg gattcttttt ttcgaaaaat gcgctacact tatagttgaa
    37741 cgaataaaaa aggagctgaa agtatgaatt cattaacaag cacttatcgt ctatcaaatg
    37801 gttacgaaat cccggtagta ggattcggca catggcaaac acctgatggg gatgtcgctg
    37861 tttcctctgt taaagaagcg ctggctgcag gttatcgcca tatcgatact gcccaaggat
    37921 acaagaatga agaaagcgtc ggccaagcga ttaaggaaag tggaattcct agagaagaga
    37981 tattcttgac aacaaaatta tggaatgcaa accacagcta tgaacttgtg atgtcttctt
    38041 ttgaagaatc attgaagaag ctccaaacag attatttaga tctgtttttg atccactggc
    38101 caaaccctgt tgcttttcgt gataattggg aacaagcaaa tgcagataca tggcgagcat
    38161 ttgaagaact gtatgaagca ggtaaaatca aagcaatcgg tgtgagcaat ttcttacccc
    38221 accatctaga tacactagca aaaacagcaa aaattatgcc gatggtcaat caagttttcc
    38281 ttgcaccagg cgaattacag acaactgtag ttgagtatgc aaaaaaacat gatatgatcc
    38341 tcgaagcata tagtccatta ggcactggta aaatcttcga tgtgcctgaa atgaaacaaa
    38401 tcgctgaagc acatgacaag acgattgcac aagttgcttt gcgttggtcg ctgcaacacg
    38461 aattccttcc tcttcctaag tcagtgacac caagcagaat caaagaaaat actgaattat
    38521 ttgattttga attaacagaa gaagaaatga aacaaatcga tcagttagat ggtgtagtcg
    38581 gcaaagcaaa agacccagat actacccaat tttaagtcga aaggtgattt tatggatcca
    38641 atcgaacgct tgaattctct ctcggaagaa gttacccaga cttttcattc cgattttgtc
    38701 tttttgatcg atgcagagaa aatccaacat tttcctgcaa gaaactggac acatgatcaa
    38761 atcatcgaag aactgaaaaa acggttcgat cattcgctaa tggtcaaacc ctggcacgaa
    38821 catgaagtca tttattcacc tgaactacct gtttttgcct tgataccgaa aaaatagaaa
    38881 gaatggaacg gattatgtat atatacataa tccgttccat tttttatttt tctttcaaaa
    38941 aacgggaaaa tacgcaaatt tttctgttgt aatttgcgta tacgcaagtt tttttgcatt
    39001 actccttttc atttggctgt ttttttctta taatcgaaag ggtatgagcg aaggagagaa
    39061 catatgaaga aaaaacagaa acaaaaatta ttaaatcaat tccgtccttc attggatggt
    39121 gtaagaggac aattattccg ttctctagaa gaagagagct tgcgacgtta tggtttgccg
    39181 ttacaagtca tgcttgatcc caaaaaccgt caaatcttaa cgatcggatt agctgggaaa
    39241 acaaatgaag atacacggat caacgtgcca atcgatgaca acttcaatac tgtattaaag
    39301 agaattcgtt caggagaaaa cgggattttc gaacgattcc gtgacaatct tcttattgaa
    39361 atcgtgtctt actggaatga tcaacgattg aaaaacgatg aaccaactgc gcaagctgtt
    39421 gcgacaccag ttacagaaga aacagtcagt gtgccagaag aaaaaacgga aaaaccaaaa
    39481 gcggacacaa ctgaaaatac acgctcaact gatacgaatg ccgctttaag ttttgctgat
    39541 ttttcaaaag ccgttgctga gtatcctaaa ttttacacag aacaaaatga acatgaagta
    39601 gttattaatg aaaaaaatgc tgatgaacct cgtaaattag caactatttc tatgactact
    39661 gaaaatgagt tcgttatcga aaaagcactc gaacggaaat acaaagtcaa attgactttg
    39721 attcctttga tcgaacaatt tgcagcgaca gcgattactg atcgataaaa attctatttg
    39781 tcataaaagg ctgggattta aaatcccagc ctttttagtc atataataat ttctacagca
    39841 agtaaaaaga aattccttct aacatcaggc tcaaatatca gcttgacctg tccaacaaat
    39901 cacctatgat cctacaatgt taggcgactt ctcttctacc tgaagatatc ttggagttgc
    39961 ctgatttctt aagatgccta accttaacta taaaaaattc taataataaa catattttct
    40021 gatagagaaa aaag
//
LOCUS       DAZUMI010000012        39683 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000012 DAZUMI010000000
VERSION     DAZUMI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.012"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..907)
                     /locus_tag="ACG81C_000502"
     CDS             complement(32..907)
                     /locus_tag="ACG81C_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5063690.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="ACG81C_000503"
     CDS             1055..1387
                     /locus_tag="ACG81C_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5063691.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="ACG81C_000504"
     CDS             1499..2707
                     /locus_tag="ACG81C_000504"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5063692.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="ACG81C_000505"
     CDS             2861..3316
                     /locus_tag="ACG81C_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063693.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="ACG81C_000506"
     CDS             3678..3953
                     /locus_tag="ACG81C_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063694.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..6044
                     /locus_tag="ACG81C_000507"
     CDS             3966..6044
                     /locus_tag="ACG81C_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5063695.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6207..8090
                     /locus_tag="ACG81C_000508"
     CDS             6207..8090
                     /locus_tag="ACG81C_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5063696.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            8102..9049
                     /locus_tag="ACG81C_000509"
     CDS             8102..9049
                     /locus_tag="ACG81C_000509"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5063697.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9069..9596
                     /locus_tag="ACG81C_000510"
     CDS             9069..9596
                     /locus_tag="ACG81C_000510"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5063698.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9659..10312)
                     /locus_tag="ACG81C_000511"
     CDS             complement(9659..10312)
                     /locus_tag="ACG81C_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5063699.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10444..11286
                     /locus_tag="ACG81C_000512"
     CDS             10444..11286
                     /locus_tag="ACG81C_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5063700.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11444..12340
                     /locus_tag="ACG81C_000513"
     CDS             11444..12340
                     /locus_tag="ACG81C_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5063701.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12343..13056
                     /locus_tag="ACG81C_000514"
     CDS             12343..13056
                     /locus_tag="ACG81C_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5063702.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13076..13594
                     /gene="msrA"
                     /locus_tag="ACG81C_000515"
     CDS             13076..13594
                     /gene="msrA"
                     /locus_tag="ACG81C_000515"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5063703.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13591..13818
                     /locus_tag="ACG81C_000516"
     CDS             13591..13818
                     /locus_tag="ACG81C_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5063704.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13970..14521
                     /gene="lepB"
                     /locus_tag="ACG81C_000517"
     CDS             13970..14521
                     /gene="lepB"
                     /locus_tag="ACG81C_000517"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5063705.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14604..15146)
                     /locus_tag="ACG81C_000518"
     CDS             complement(14604..15146)
                     /locus_tag="ACG81C_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5063706.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15383..15781)
                     /locus_tag="ACG81C_000519"
     CDS             complement(15383..15781)
                     /locus_tag="ACG81C_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5063707.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16041..16901
                     /gene="ylqF"
                     /locus_tag="ACG81C_000520"
     CDS             16041..16901
                     /gene="ylqF"
                     /locus_tag="ACG81C_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5063708.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            16894..17661
                     /locus_tag="ACG81C_000521"
     CDS             16894..17661
                     /locus_tag="ACG81C_000521"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5063709.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            17717..18574
                     /gene="dprA"
                     /locus_tag="ACG81C_000522"
     CDS             17717..18574
                     /gene="dprA"
                     /locus_tag="ACG81C_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5063710.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            18696..20774
                     /gene="topA"
                     /locus_tag="ACG81C_000523"
     CDS             18696..20774
                     /gene="topA"
                     /locus_tag="ACG81C_000523"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5063711.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            20743..22119
                     /gene="trmFO"
                     /locus_tag="ACG81C_000524"
     CDS             20743..22119
                     /gene="trmFO"
                     /locus_tag="ACG81C_000524"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5063712.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            22317..23222
                     /gene="xerC"
                     /locus_tag="ACG81C_000525"
     CDS             22317..23222
                     /gene="xerC"
                     /locus_tag="ACG81C_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5063713.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            23259..23807
                     /gene="hslV"
                     /locus_tag="ACG81C_000526"
     CDS             23259..23807
                     /gene="hslV"
                     /locus_tag="ACG81C_000526"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5063714.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            23821..25221
                     /gene="hslU"
                     /locus_tag="ACG81C_000527"
     CDS             23821..25221
                     /gene="hslU"
                     /locus_tag="ACG81C_000527"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5063715.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            25239..26030
                     /gene="codY"
                     /locus_tag="ACG81C_000528"
     CDS             25239..26030
                     /gene="codY"
                     /locus_tag="ACG81C_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5063716.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            26112..26987
                     /locus_tag="ACG81C_000529"
     CDS             26112..26987
                     /locus_tag="ACG81C_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5063717.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACG81C_000530"
     CDS             complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACG81C_000530"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5063718.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            27884..28318
                     /locus_tag="ACG81C_000531"
     CDS             27884..28318
                     /locus_tag="ACG81C_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5063719.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            28484..30529
                     /gene="parE"
                     /locus_tag="ACG81C_000532"
     CDS             28484..30529
                     /gene="parE"
                     /locus_tag="ACG81C_000532"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5063720.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            30541..32991
                     /gene="parC"
                     /locus_tag="ACG81C_000533"
     CDS             30541..32991
                     /gene="parC"
                     /locus_tag="ACG81C_000533"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5063721.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            33268..35499
                     /gene="pflB"
                     /locus_tag="ACG81C_000534"
     CDS             33268..35499
                     /gene="pflB"
                     /locus_tag="ACG81C_000534"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5063722.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            35743..36504
                     /gene="pflA"
                     /locus_tag="ACG81C_000535"
     CDS             35743..36504
                     /gene="pflA"
                     /locus_tag="ACG81C_000535"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5063723.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            36589..37515
                     /locus_tag="ACG81C_000536"
     CDS             36589..37515
                     /locus_tag="ACG81C_000536"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5063724.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            37697..38590
                     /locus_tag="ACG81C_000537"
     CDS             37697..38590
                     /locus_tag="ACG81C_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5063725.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            38724..38849
                     /locus_tag="ACG81C_000538"
     CDS             38724..38849
                     /locus_tag="ACG81C_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5063726.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(38983..39615)
                     /locus_tag="ACG81C_000539"
     CDS             complement(38983..39615)
                     /locus_tag="ACG81C_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063727.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        13882 a         6821 c         8332 g        10648 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     6001 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     6061 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     6121 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     6181 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     6241 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     6301 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     6361 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     6421 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     6481 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     6541 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     6601 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     6661 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     6721 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     6781 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     6841 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     6901 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     6961 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     7021 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     7081 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     7141 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     7201 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     7261 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     7321 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     7381 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     7441 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     7501 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     7561 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     7621 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     7681 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     7741 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     7801 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     7861 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     7921 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     7981 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     8041 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     8101 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     8161 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     8221 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     8281 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     8341 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     8401 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     8461 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     8521 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     8581 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     8641 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     8701 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     8761 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     8821 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     8881 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     8941 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     9001 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     9061 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     9121 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     9181 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     9241 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     9301 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     9361 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     9421 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     9481 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     9541 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     9601 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     9661 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     9721 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     9781 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     9841 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     9901 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     9961 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
    10021 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
    10081 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
    10141 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
    10201 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
    10261 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
    10321 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
    10381 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
    10441 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
    10501 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
    10561 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
    10621 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
    10681 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
    10741 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
    10801 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
    10861 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
    10921 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
    10981 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
    11041 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
    11101 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
    11161 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
    11221 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
    11281 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
    11341 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
    11401 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
    11461 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
    11521 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
    11581 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
    11641 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
    11701 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
    11761 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
    11821 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
    11881 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
    11941 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
    12001 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
    12061 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
    12121 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
    12181 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
    12241 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
    12301 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
    12361 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
    12421 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
    12481 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
    12541 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
    12601 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
    12661 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
    12721 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
    12781 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
    12841 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
    12901 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
    12961 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
    13021 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
    13081 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
    13141 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
    13201 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
    13261 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
    13321 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
    13381 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    13441 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    13501 tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    13561 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    13621 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    13681 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    13741 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    13801 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    13861 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    13921 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    13981 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    14041 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    14101 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    14161 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    14221 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    14281 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    14341 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    14401 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    14461 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    14521 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
    14581 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
    14641 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
    14701 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
    14761 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
    14821 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
    14881 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
    14941 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
    15001 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
    15061 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
    15121 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
    15181 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
    15241 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
    15301 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
    15361 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
    15421 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
    15481 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt
    15541 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
    15601 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
    15661 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
    15721 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
    15781 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
    15841 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
    15901 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
    15961 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
    16021 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
    16081 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
    16141 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
    16201 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
    16261 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
    16321 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
    16381 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
    16441 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
    16501 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
    16561 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
    16621 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
    16681 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
    16741 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
    16801 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
    16861 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
    16921 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
    16981 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
    17041 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
    17101 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
    17161 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
    17221 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
    17281 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
    17341 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
    17401 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
    17461 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
    17521 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
    17581 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
    17641 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
    17701 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
    17761 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
    17821 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt
    17881 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
    17941 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
    18001 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
    18061 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
    18121 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
    18181 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
    18241 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
    18301 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
    18361 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
    18421 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
    18481 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
    18541 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
    18601 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
    18661 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
    18721 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
    18781 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
    18841 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
    18901 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
    18961 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
    19021 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
    19081 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
    19141 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
    19201 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
    19261 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
    19321 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
    19381 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
    19441 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
    19501 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
    19561 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
    19621 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
    19681 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
    19741 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
    19801 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
    19861 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
    19921 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
    19981 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
    20041 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
    20101 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
    20161 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
    20221 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
    20281 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
    20341 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
    20401 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
    20461 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
    20521 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
    20581 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
    20641 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
    20701 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
    20761 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
    20821 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
    20881 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
    20941 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
    21001 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
    21061 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
    21121 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
    21181 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
    21241 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
    21301 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
    21361 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
    21421 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
    21481 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
    21541 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
    21601 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
    21661 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
    21721 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
    21781 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
    21841 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
    21901 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
    21961 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
    22021 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
    22081 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
    22141 agacggatag tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg
    22201 aggaattgcg ccaattgtga acaaacttag aaatattggt taaattctga caatttattt
    22261 gtctttttat aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga
    22321 cagaagccac gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag
    22381 aaaaaacgaa agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat
    22441 ctggagacgc tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat
    22501 tagctgatca tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac
    22561 gttcgtttta tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg
    22621 ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag
    22681 cactttttga cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg
    22741 aagtccttta cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca
    22801 ttgattttga ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc
    22861 ctctaggttc ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat
    22921 tgatgacaaa atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa
    22981 tcacgcctac tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata
    23041 gtgatatcca tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg
    23101 cagatttgcg gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct
    23161 atgcccatgt aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt
    23221 aaaaataagg aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt
    23281 caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag
    23341 taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca
    23401 atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa
    23461 aatttgaagg caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag
    23521 ctcaagaatg gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga
    23581 atgataaaga aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta
    23641 tcttagctat cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact
    23701 atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag
    23761 atatctgtat ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat
    23821 atgtatgaat taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta
    23881 ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg
    23941 cagttagatg aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct
    24001 acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt
    24061 gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca
    24121 atggttcggg atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt
    24181 gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt
    24241 atcaaaaaag aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat
    24301 gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat
    24361 cgccgtacag tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt
    24421 gaagttgaag accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg
    24481 atcgacctaa atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta
    24541 acagtcaaag aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat
    24601 gctgatatcc atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat
    24661 gaaatcgata agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc
    24721 caaagagaca tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc
    24781 caaacagatc acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat
    24841 ctaattccag aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct
    24901 gaagattttg tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg
    24961 ttgatcggta cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct
    25021 caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc
    25081 attttggaaa aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa
    25141 ataacgatca cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat
    25201 ttaagtcgct atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa
    25261 cccgcaagat aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc
    25321 cttacgataa aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag
    25381 gaagtgacgg agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca
    25441 aaacaatgtt tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga
    25501 tgaaaactga agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa
    25561 gagagaaata tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt
    25621 tgggtacgat tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag
    25681 cagaatatgg agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg
    25741 aagcagatgt acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg
    25801 aattgaaggc tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg
    25861 cttctaatat tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa
    25921 aattggaatc agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa
    25981 aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg
    26041 actggggcag atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg
    26101 gaggaatggc aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac
    26161 acggtgcgga attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag
    26221 gtgatccagc gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa
    26281 aagatgatca gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc
    26341 gggatatgga ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga
    26401 gtacgccgga gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat
    26461 taggaggaga cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt
    26521 tcttttctat cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg
    26581 aagatttcta tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc
    26641 cattcatcga tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc
    26701 gtgaactttt tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca
    26761 ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag
    26821 ggatctggtc tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa
    26881 tcgctgacac ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag
    26941 gtgcccatga aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg
    27001 agaagttagc agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata
    27061 agggagtgtg acatcgattt gtcacactcc cgttttatct ctttggaatg aaaaagtatc
    27121 aggatttttt cttgctgcta ttcaaaccaa acgggattcg actttcagta tggtttctga
    27181 tacgcttgat attgtcttta tgccgataaa agataaagga agaaactaaa aaggcaatga
    27241 tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc tacataagga aggacgatcg
    27301 tagataaagt gatcaatact gcactgatca tactgctcaa actcaccata cttgtacaaa
    27361 ataaactaac gataaagatg atacaagaat aaacaaaaaa tacaggatta aatcctaaaa
    27421 gcattcctgc acttgtcgca acagcttttc ctcctttgaa cccagcaaaa atcggaaatg
    27481 tatggccaag aatggcgcat accccaaacc aaagagggtt aatcccagaa aggtggaaga
    27541 tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc cataagcagg acaacaatcc
    27601 cggctgtttt gcctaataca cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt
    27661 gtcggatatc ttttttgaaa aacaatttgc caatccagac accggaagga atagaaccta
    27721 aaagatatgc taaaataagt aataagatca atttcatttt ttaccctctt tcaagttgaa
    27781 aactatcctt tattttagca tgaataggta acaggaacaa ttgaaaaaat gaaagataca
    27841 agtataatag atggaataaa aagaaaagca aggggtggca gaaatgtctg taaaaaatcc
    27901 aacacaagaa acgattttca actatttgga agaagcaaaa aatattgccg tcgtaggctt
    27961 gagcaataaa ccagaacgaa caagttacat ggttgcacag gtattgcaag acaatggata
    28021 taaaatcata ccagtaaatc cattattggc tggtgaagaa attctaggcg agaaagtcta
    28081 tgcgaatttg acagaaatcg atgatccaat cgatatcgta gatgtattta gacggagtga
    28141 atttttacct gaaatagcag gtgatttttt gaaaactgat gctaaagtgt tctgggcaca
    28201 attagggatt gaaaacaaag aagcagctga gatgctaaga aaagcaggac gtacagatat
    28261 cgtaatggat cgttgtatta agattgaact tggtaagttg aaagaaaacc aaaaataaaa
    28321 atgaccacaa agaagagatg atcgatagag gggaatgtcg aaaaagcggt taggagataa
    28381 ttaaaattga tttctcgtct accctctttt ttttcacgac cttttctagt atgataatcg
    28441 tggtgcggtt ttgccgcaaa gtattattga aggagcgttt gccttggcaa aaaaagtaaa
    28501 caacgaatac aatgactcgt ccatccaagt attggaagga ttagaggcag ttagaaaaag
    28561 accagggatg tacattggat ccactgacag ccgaggcttg catcatttag tctatgaaat
    28621 cgtcgataat gcagttgacg aagcattatc aggttatggc agtgagattg atgtgaccat
    28681 ccatgaagac aatagtataa ctgttgcaga tagcggacgt gggatgcctg taggaatgca
    28741 tgcttccgga ataccaacag ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt
    28801 tggtcaagga gggtacaaaa cttctggtgg actgcacggt gtaggagcca gtgtggtaaa
    28861 tgccttatcg aaatggctaa ttgtaacgat cgttagagat ggcgtagagt atcagcagaa
    28921 atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc
    28981 aaacgggacg actgtccatt ttttaccaga tgatacaata ttcagtacaa cgaaattttc
    29041 atatgaaatt ttagccgaac ggttaagaga atctgccttt cttctaaaag gcgttaaaat
    29101 ctctctttct gatcttcgtg gagaagaacc agtcaaggaa atttttcatt acgaagaagg
    29161 aatcaaagaa tttgttgatt atctgaatga agaaaaagat acattgacac cagttgttta
    29221 tttttcagga gaaaaagaag gtatcgaagt agaggtcgct tatcaataca atgacggtta
    29281 ttcagaaaat gttttatctt ttgtcaataa cgttcggaca aaagatgggg gaacccatga
    29341 agctggtatg aaagcggcga tgacgaaatc ttataatgaa tatgcgagaa aagttggttt
    29401 gctgaaagaa cgggacaaga atctggaagg tagtgatttc agagaagggt tagctgctgt
    29461 actttctatc cgtgtacctg aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact
    29521 tggtacgcca gttgctcgta ccgtagtaga taacgtcatc agtgaacaaa tgggctttta
    29581 tttgcaggaa aatagcgaaa tgagccagat gttggtccgt aaagcaatca aagccagaga
    29641 agcgagagaa gcagcacgta aagccagaga agaaagccga aatgggaaga agcgtaaaaa
    29701 aggcgaatct cttctttcag gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa
    29761 tgaattgtat ttagtcgaag gggattctgc tggcggatct gctaaacaag gaagagacag
    29821 aaagttccaa gcaattctac ctttacgagg aaaagtcatc aataccgaaa aagctaaaat
    29881 gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc tatacgattg gtgcaggagt
    29941 cggtcctgag ttttctatcg aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc
    30001 ggatacagac ggcgcccata ttcaagtatt actgcttact ttcttttatc gttatatgaa
    30061 accgctgatt gaagctggga aagtatatat cgcgttaccg cctttatata aagtatccaa
    30121 aggacaagga aagaagcaag tcattgaata tgcgtggaca gatgacgaat tggcagcaat
    30181 gattaaaaaa gtcggcaaag gatatatgct gcagcgatat aaaggacttg gagagatgaa
    30241 tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc cgtacgttga tccgcgtccg
    30301 tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga
    30361 acctcgaaga aaatggatcg aaaatcacgt gcaatttacg ctcgaagaag atggcagtat
    30421 cttagataaa aaagaggata ctgaaatctc tccttcagtt tctaatgatt tattagatga
    30481 agaacgagca gataaaaatg aaaacaatca gttatttgaa gtagaatagg aggtcacctg
    30541 atggaaaatc gacaagaagt acaagaatta actttagaag aagtgatggg tgaccgcttc
    30601 ggacgttatt ccaaatatat tatccaagaa cgtgcactgc ctgatatccg tgatggattg
    30661 aagccagtgc aacgccggat attatttgcc atgaacaaag atggcaatac gtatgacaaa
    30721 ggattccgta aatcagcaaa atctgtcgga aatatcatgg ggaattatca tcctcatggt
    30781 gacatcagta tttatgaagc gatggtccgc atgagccaag actggaagtt gcgggaagta
    30841 ctgatcgaaa tgcacgggaa taacgggagt atggacggtg atccacctgc tgcgatgcgt
    30901 tatactgaag ccagattatc caagttaagt ggagagatgc tagcagatat cgagaaagaa
    30961 acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag aaccaacagt ccttccagct
    31021 cgatacccaa accttctcgt caatggatcg acaggtatat ctgctggata tgcaacagaa
    31081 atcccgactc ataacttagc tgaagtgatc gatggcacaa tctatatgat cgatcatcca
    31141 caagcgagcc ttgaaaaatt gatggaatat attcctggac ctgactttcc aacaggaggg
    31201 atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt
    31261 cttcgttcga aaacaaagat agaggcaatg aagggtaata aacagcaaat cgtgatcaca
    31321 gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa aaatggatga gatccgtttg
    31381 aataagaaaa tcgatggaat agctgaagta cgagatgaaa gtgatagaac aggtctgcaa
    31441 attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa ttttgaatta tttgttcaaa
    31501 aacactgagc tacagatcaa ttacaatttc aatatggtcg ctatcgacca tatgactccg
    31561 catcaagttg gactgaaaga cattttaaga agttatatcg agcatcgaaa gcaagtcata
    31621 acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa gacagcatat cgtagaagga
    31681 ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac
    31741 aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat ttacagaaga acaagcagaa
    31801 gcaattgtca cattgcaatt ataccgtttg acgaacacgg atatcacaga gcttcaaaag
    31861 gaatccgaaa gcttgattgc gcaaatcaca gagttgaata aaatcctttc aaatgacaaa
    31921 gagcttttct ctgttatgaa aaaagaattg cgagaagtga aaaaaaatta ttcttccgct
    31981 cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg
    32041 gcacaagaag acgtaattgt cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt
    32101 cgttcttata gtgcttccaa accagaagaa atcgggatgc gagaaggcga ctacttgcta
    32161 tactcaggtg aattgagtac attggaccat gttcttctga tcacaaataa agcaaatgtg
    32221 atctaccgcc ctgtccatga actacccgat ttgaaatgga aagatgctgg tgagcacatt
    32281 tctcaaacaa tctccaatct gtctgtagac gaatcgattt tagctgtttt cccatatcaa
    32341 aaaattcaag cagagaaaac atttgtattc atcagcaaga atggattgat caagcaaaca
    32401 cgaatgacag agttcgaacc atggcgcacc tataagagcc gtccgctcag ctgtatgaag
    32461 ctaaaggcgg aagaagatga actgattgct gtgtatctga aagaagatca gccaggctta
    32521 gatgttttct tagtaacgca tcaaggaatg ggtttgcgct atcctttaac agatgttccc
    32581 gttgttggag caaaggcagc tggagtgaag tctatgaatt tgaaagatgg agataggatt
    32641 atcaacgggc tgcttgtgtt agcagaaggt gatacgccga tcgtaatcgt tactcagcga
    32701 ggagctgtta aacgcatgct tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga
    32761 gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga
    32821 agatcaaaag aaataacctt gatgaatcaa aaagggcagc agttaacgat tgacccaact
    32881 gatttcccta taggtgatcg aacatcgaat ggatcatttg cgatggatga gaaaaaaggt
    32941 ggagaagtca tcgaagttat cgatgctcct gagattttca tcgagtcgta actgttgtag
    33001 tattttaatg aagagacgcc atctgtatta atacaggtgg cgtctctttt tcattcattt
    33061 ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg
    33121 gtatgtcaac gttgatttaa atatatgtaa ataaaacata aaaattgtta aataaaaaac
    33181 aagaaaaccc ttgcaaagat agggtgctgt gttatactaa tcacgtaaaa actgttacat
    33241 aaaacagaaa tttaggaggt ccatacaatg gagcaatgga atggatttaa aggtaccaaa
    33301 tggaaagaag gcgtagacac tagagacttt attcaaaata actacactga atatcgcggt
    33361 gatgacagct ttttggaagg cccaacagaa gcaacagaaa aattatggga tgaattgcaa
    33421 aagttaaatg atatccaatt cgagcgtaac ggtgtctatg atatggacac agaaatcgta
    33481 tcaactatta cttcacacga tgcaggctat ttggataaag atttggaaaa aatcgtcggt
    33541 ctacagacag acaaaccttt gaaacaagca ttcatgccgt tcggcggaat caatatggct
    33601 aacaatgcgc taaaaagcaa tggctataca ccaaacgatg aattgacaca tattttcact
    33661 gactggcgca aaacacataa tcaaggtgta tttgacgctt atactgctga aatgcgtgct
    33721 gctcgtaaaa acaaaatcat tacagggctt ccagacgctt acggtcgcgg acgtatcatc
    33781 ggtgactatc gtcgtgtggc attgtatgga atcgactatt tgatggaaca aaagaaaaaa
    33841 gatcatgata tgactggtta tcatacaatg agtgaagaag tgattcgttt acgtgaagaa
    33901 atcactgaac aatatcgtgc attagcgcaa atgaaagaaa tggctgcgaa atatggctat
    33961 gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc aatggctata cttcggatat
    34021 ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc
    34081 cttgatatct atatccaacg tgatttggat aatggagtaa tcacagaaaa agaagcacaa
    34141 gaattgatcg accacttagt aatgaaatta cgtatggtca aattcgcgcg tacacctgaa
    34201 tataaccaat tattctcagg attccctatc tgggctactt tatccattgc aggtatggga
    34261 ttagatggtc gttcattagt tacaaagaat gatttccgtc tgcttcatac attaacaaat
    34321 atgggacctt caccagaacc aaacttgact gttttgtatt ctgaacacct tccagaagga
    34381 ttcagaacat atgctagcag aatcgctatc gaaagttctt cgattcaatt cgaaaatgat
    34441 aatcagcttc gtgaacaatg gggttctgat gattgtgcga ttgcttgctg tgtatcagga
    34501 acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg ctaacttggc taaaacacta
    34561 ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg cccaagttgg accagaatat
    34621 cgtccaatca catcagaaat actagacttt gaagaagtga aacataactt catgaatatg
    34681 atggattggt tagcagaact atatgtcaat acgttgaaca tcattcatta tatgcatgat
    34741 aaatattctt atgaagcagc tcagctggct ttgatggatt ctgacttgca acgcacattt
    34801 gctacaggta ttgctgggat ctctcatgcg gcagacagct tgtcagcgat caaatatgca
    34861 aaagtaaaag caatccgtaa tgaaaaagga atagcagttg attttgaagt ggatggcgac
    34921 ttccctaaat acggaaatga tgatgaccgt gcagatgaat tagtttcatg gatcttggaa
    34981 tacttcatgg gacaaatcaa acgtcataaa acttaccgca actcaaaacc aacaacttca
    35041 ttattgacga ttacttcaaa tgttgtttac ggtaaaaata caggaaatac tcctgatggc
    35101 cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt
    35161 gggttattag ctagcctgaa ctcaacagct aaaatgaatt acggctgtgc attggatgga
    35221 atttcaaata cccaaacaat caatccttca ggtcttggaa aagaacaaga tacaagaatc
    35281 gaaaacttgc gtaatgtctt agatggatac ttctcaaaag gagcttatca tttgaatgtc
    35341 aatgtattca gtaatgattt attgcgtgat gcaatggaaa atccaatgaa atatccgaac
    35401 ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg acttgacaag agaacaacaa
    35461 ttagatgtct tgatgagaac ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt
    35521 tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg
    35581 cttatttgaa agaaacgatt tacaggggca ggacttgtat gttccgtccc tgtaaatttt
    35641 acacataacg aagcggcaat tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta
    35701 acttgtactt aaaatgaaat caaagaaaag atgtgaactt aaatggaaga aaaaacaatc
    35761 ggctatgttc attctatcga aactttcggt tcagtagacg gacctggact gcgctttgtc
    35821 gtgtttatgc aaggatgccg catgcgttgc caattttgtc acaatccaga tacttggaat
    35881 attggcggag ggaaagaata cacagcagat gaacttttag ataaagcaga acgcttcaga
    35941 ccttactggg gagataaagg tggtattact gttagtggtg gagaacctct attgcagatc
    36001 gattttttga tcgaactatt taaaaaagca aaagagcgga agatgcatac tacattggat
    36061 acatgcggaa aaccttttac gtatgaagaa cctttctttt cacgttttca agaattgatg
    36121 aagtatacag atttactgtt atttgacatc aaacacatcg ataatgaaga gcataaaaaa
    36181 ctaacgcatt gggacaatga aaatatttta gaaatggctc aatatttgtc aaaaatcaac
    36241 aaaccggttt ggattcgcca tgtactagtt cctgagcgaa gtgattatga tgaatatttg
    36301 atccgactag acaactttat tcaaaccttg tcaaatgtag acagagtaga gattcttcct
    36361 tatcatacga tgggaaaata caagtgggaa acacttggtt tgaaatatcc attagaaggg
    36421 atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac gattgcttca tacagaagag
    36481 tatacaaaat atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc
    36541 gtttttttgc tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt
    36601 ttagtttttg gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca
    36661 cacttgcaaa atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa
    36721 gaaacacaat acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct
    36781 aaagcagaaa atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca
    36841 gatatcagcg aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa
    36901 acagctaatc cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta
    36961 aaactatata aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca
    37021 gctatcattt cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca
    37081 gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg
    37141 ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca
    37201 aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt
    37261 aatgaagtga tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca
    37321 aatggctacg atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta
    37381 cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac
    37441 aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg
    37501 gctgtattta gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg
    37561 atataaaaag aaacatagaa catactaaag gccgaagaaa tggaggagaa taacagtcaa
    37621 ttatgactct tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat
    37681 agaaaagaga acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact
    37741 gaccgatgat ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga
    37801 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
    37861 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
    37921 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
    37981 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
    38041 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
    38101 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
    38161 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
    38221 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
    38281 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
    38341 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
    38401 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
    38461 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
    38521 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
    38581 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
    38641 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
    38701 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
    38761 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
    38821 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
    38881 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
    38941 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
    39001 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
    39061 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
    39121 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
    39181 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
    39241 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
    39301 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
    39361 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
    39421 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
    39481 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
    39541 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct
    39601 acgttgaatt tccataatat ctccaaatta aattgataag tatataaagt ataataacaa
    39661 attgatgagt atatgtcgat gtt
//
LOCUS       DAZUMI010000013        37244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000013 DAZUMI010000000
VERSION     DAZUMI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.013"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..839
                     /locus_tag="ACG81C_000540"
     CDS             <1..839
                     /locus_tag="ACG81C_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5063728.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1192..2040)
                     /locus_tag="ACG81C_000541"
                     /pseudo
     CDS             complement(1192..2040)
                     /locus_tag="ACG81C_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2193..3305
                     /locus_tag="ACG81C_000542"
     CDS             2193..3305
                     /locus_tag="ACG81C_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5063729.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="ACG81C_000543"
     CDS             complement(3383..3676)
                     /locus_tag="ACG81C_000543"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5063730.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="ACG81C_000544"
     CDS             3844..4158
                     /locus_tag="ACG81C_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5063731.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="ACG81C_000545"
     CDS             4179..6158
                     /locus_tag="ACG81C_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5063732.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="ACG81C_000546"
     CDS             6171..6497
                     /locus_tag="ACG81C_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5063733.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="ACG81C_000547"
     CDS             6513..7031
                     /locus_tag="ACG81C_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063734.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="ACG81C_000548"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="ACG81C_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5063735.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="ACG81C_000549"
     CDS             complement(8467..9972)
                     /locus_tag="ACG81C_000549"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5063736.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="ACG81C_000550"
     CDS             complement(10064..10654)
                     /locus_tag="ACG81C_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5063737.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="ACG81C_000551"
     CDS             10833..12479
                     /locus_tag="ACG81C_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5063738.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="ACG81C_000552"
     CDS             12528..13247
                     /locus_tag="ACG81C_000552"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5063739.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="ACG81C_000553"
     CDS             13726..14673
                     /locus_tag="ACG81C_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063740.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="ACG81C_000554"
     CDS             14685..15605
                     /locus_tag="ACG81C_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5063741.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="ACG81C_000555"
     CDS             15646..17103
                     /locus_tag="ACG81C_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5063742.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="ACG81C_000556"
     CDS             17448..18062
                     /locus_tag="ACG81C_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5063743.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="ACG81C_000557"
     CDS             18055..19779
                     /locus_tag="ACG81C_000557"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5063744.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="ACG81C_000558"
     CDS             19776..21227
                     /locus_tag="ACG81C_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5063745.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21699..23852)
                     /locus_tag="ACG81C_000559"
     CDS             complement(21699..23852)
                     /locus_tag="ACG81C_000559"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5063746.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23854..24900)
                     /locus_tag="ACG81C_000560"
     CDS             complement(23854..24900)
                     /locus_tag="ACG81C_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5063747.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25050..26339
                     /locus_tag="ACG81C_000561"
     CDS             25050..26339
                     /locus_tag="ACG81C_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5063748.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26352..29054
                     /locus_tag="ACG81C_000562"
     CDS             26352..29054
                     /locus_tag="ACG81C_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5063749.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29039..29896
                     /locus_tag="ACG81C_000563"
     CDS             29039..29896
                     /locus_tag="ACG81C_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5063750.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29967..30824)
                     /locus_tag="ACG81C_000564"
     CDS             complement(29967..30824)
                     /locus_tag="ACG81C_000564"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5063751.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31035..33206
                     /locus_tag="ACG81C_000565"
     CDS             31035..33206
                     /locus_tag="ACG81C_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5063752.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLSLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33274..33615
                     /locus_tag="ACG81C_000566"
     CDS             33274..33615
                     /locus_tag="ACG81C_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5063753.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33891..35645)
                     /locus_tag="ACG81C_000567"
     CDS             complement(33891..35645)
                     /locus_tag="ACG81C_000567"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063754.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35656..36492)
                     /locus_tag="ACG81C_000568"
     CDS             complement(35656..36492)
                     /locus_tag="ACG81C_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5063755.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36494..37177)
                     /locus_tag="ACG81C_000569"
     CDS             complement(36494..37177)
                     /locus_tag="ACG81C_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5063756.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12012 a         6553 c         7639 g        11040 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tctacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctgaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa aaaagccaat
    21241 gtaatgaaat aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact
    21301 tgaggcaagg cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg
    21361 gtattaagcc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata
    21421 ctcgaaacta aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc
    21481 tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa
    21541 tactcaggtc agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat
    21601 ctgcggtaat aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct
    21661 tattacttga tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa
    21721 aaaggcaaat cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag
    21781 atcgtgccgc ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc
    21841 atccgtttga ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc
    21901 aattcctttc catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca
    21961 tattcaccgg ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg
    22021 gccatacttc cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga
    22081 gtcatcaact gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag
    22141 taaggatagt gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc
    22201 atctcactca tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga
    22261 ttgcacagct caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt
    22321 cctgaaaaat cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct
    22381 tgtccccaac gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc
    22441 ataaatcctg actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc
    22501 ttcgtcattt cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa
    22561 gcatagtcta atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc
    22621 aagtaatctt ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct
    22681 ttttccattg ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca
    22741 tctgcaatga ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc
    22801 cattttggca aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg
    22861 tatttttcta cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac
    22921 ccattatttg tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt
    22981 ttttgcccac taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa
    23041 ttatgataaa acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa
    23101 taggactgcc attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct
    23161 agttccaggt tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt
    23221 tgtttttttg tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct
    23281 aactcatcaa ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga
    23341 tcttctgaac cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag
    23401 agctgatggc gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata
    23461 gataagattc cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga
    23521 cagatcgaca gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca
    23581 tggaaaaggg tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa
    23641 tcacccatcc aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca
    23701 ggatgaaacc accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca
    23761 aaaggaacgc cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt
    23821 gcatgtctag tatctatttg accgatattc attttatttc tcctttgttg attgtctttt
    23881 gatgaatgaa actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac
    23941 caaagcatct cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg
    24001 cgggtcaaca agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg
    24061 gatatgataa ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc
    24121 acaaacaaaa gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc
    24181 gcttttgtca tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta
    24241 accaaggtaa cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat
    24301 ttttgaatgt ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc
    24361 ggaaagaacg gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa
    24421 ttctttcaat gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata
    24481 aatcaatccg tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag
    24541 cgtcgtcatc atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa
    24601 atcaccaaca gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt
    24661 aggagaagca agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac
    24721 gcgataaata agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct
    24781 tactttatag gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc
    24841 accagttaaa atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat
    24901 ataaacacct cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa
    24961 taaaaagata ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag
    25021 atatatcaaa tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc
    25081 agaaatttat ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga
    25141 attttacagc agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa
    25201 ccaccttttt attgacggga gacattccag cgatgtggtt aagagactct actgcacaag
    25261 ttcgtcctta tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt
    25321 tagtcaaaaa gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag
    25381 aagctaacgg tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa
    25441 gaaaatatga aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa
    25501 atacaggaaa aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg
    25561 acgtgttcaa aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga
    25621 caagaaaaga agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa
    25681 tgacatggtc tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat
    25741 ccaatatgtt tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg
    25801 atgataaaga aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc
    25861 agaatcacgg acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg
    25921 gaatgggaaa cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt
    25981 acttgggata ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga
    26041 gtaaagaaaa tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata
    26101 cgccagaaaa atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca
    26161 aatctgaaaa agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta
    26221 tgcacgaagg attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg
    26281 caaatatgat gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg
    26341 aggtatcgta aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat
    26401 ggtacatggc ttacgaacag catcacatgc gactgattga attgatagac gatctactag
    26461 agctatttga agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt
    26521 tagatgacta tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg
    26581 aaggaaaatt gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg
    26641 aatcgaacgt gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg
    26701 tcatgttagg ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga
    26761 aacaagctgg tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata
    26821 atcaagtttt ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc
    26881 ctgatcaaac agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga
    26941 ttccagtaga aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac
    27001 aatatgcctc tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa
    27061 aagatatatc aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc
    27121 attctaattt tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct
    27181 cagtagaagg agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag
    27241 catctgctcg ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata
    27301 ttacagaacc acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat
    27361 tagattatgc ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt
    27421 cagtcgattc tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa
    27481 agtatcttgc acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc
    27541 caaaagacag ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag
    27601 cagaaataac catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat
    27661 atcaagagtt agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca
    27721 ctataccagc tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc
    27781 gtttccgtgt gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc
    27841 cagcattttc ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg
    27901 aaacaatgat tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg
    27961 aaaaaaatgg taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc
    28021 atatttttga agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg
    28081 atcagccaat cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac
    28141 cggaaatagc aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc
    28201 gtttagaaaa agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca
    28261 aagaaaaacg aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa
    28321 tcgactttga aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc
    28381 ctacaaagtt acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc
    28441 caaaccatgt gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg
    28501 tcaatattca tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg
    28561 aagtaacaga agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg
    28621 attggggata ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca
    28681 gcattgaact acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac
    28741 agattccttt ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac
    28801 aatatttaac gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta
    28861 gtgataaagt agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa
    28921 ctaaagacaa tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg
    28981 ttgtcccaat aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat
    29041 gtatggcgga atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga
    29101 aattttagaa aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt
    29161 tgaatttttt gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg
    29221 tatcgacaaa acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc
    29281 acaattcgat tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac
    29341 ggatgtcaat gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag
    29401 ttgtatttac cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat
    29461 tttttcaggg atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga
    29521 ccattatgaa ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc
    29581 gtctattgag gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg
    29641 ggctattcag gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc
    29701 tgaaaagatt attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg
    29761 tcagcaattt gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat
    29821 cgttcgttgg ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca
    29881 cgaacatcaa aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc
    29941 tgataagact ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact
    30001 attttttctt gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt
    30061 tcagctgttc tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa
    30121 tgaactctga tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca
    30181 gaaagtactt tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg
    30241 accctttttc tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg
    30301 gcaatttctc cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt
    30361 cttccaagta tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct
    30421 ttgtccaagc ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa
    30481 tgattgagta aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc
    30541 ttatccacta taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct
    30601 aattgaactt cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg
    30661 ccctttactt catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata
    30721 cgcaaaaagt gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt
    30781 gttgtttcag gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca
    30841 gtgcaattgc agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt
    30901 tctgttcatt cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac
    30961 ttaagcagga agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac
    31021 aggagaagaa gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca
    31081 gtttttggcg ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct
    31141 ggaaaaaata tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga
    31201 caagtatcta cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat
    31261 taagccaatc gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc
    31321 aaaaaggaac atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct
    31381 cgacagaaga taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg
    31441 cagcagtccg aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc
    31501 agcagcttgc caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg
    31561 aattattttt ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt
    31621 atcttaacaa tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt
    31681 attttggagt agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc
    31741 tgaaaggtcc tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa
    31801 acacagtact aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag
    31861 aaagccagac agatatcagc gcgtatctta atgacacata cgataattca gattctggtt
    31921 atcgttatcc ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg
    31981 atgaagaaga tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc
    32041 aaaagcaatt agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg
    32101 gtgccatggt ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac
    32161 tagtaggacg aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc
    32221 gttcaccagg atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat
    32281 acaagccttc cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg
    32341 acgggacata ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgagtttgc
    32401 ctgctgtatg gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat
    32461 tcggcctgcc tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa
    32521 aaggagtatc tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga
    32581 tgtatacgcc gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata
    32641 acacaaacaa aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca
    32701 tgcttcttgg tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga
    32761 tagcagggaa aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat
    32821 ggatgatcgg ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca
    32881 gcaagctgca ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag
    32941 cggcagaagg catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg
    33001 ccacaggagg acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag
    33061 agtctggaaa ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga
    33121 aaaacttcgg cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc
    33181 tctggcggaa atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata
    33241 gagagtaact actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat
    33301 caaatcgaac gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag
    33361 aaagtacaag caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc
    33421 gaattacaag aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca
    33481 caagaaatgg ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag
    33541 caaggcttca gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac
    33601 ctttacagcg aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc
    33661 ttgagcgatg acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc
    33721 aagaggtcgt gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct
    33781 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta
    33841 ttcagaaata ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt
    33901 ttcaagcggt agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg
    33961 atttttcctt catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga
    34021 gccacaataa aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc
    34081 tctgactggt tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt
    34141 atcaacgctc gtgcaatgga gatacattgc ttttggccac cagacagctt gttgccatgt
    34201 tcgcctacta aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca
    34261 gcttcgatga cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc
    34321 tctttgatcg aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc
    34381 acacttcgca tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc
    34441 ggctggataa atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca
    34501 acaaaagcaa ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg
    34561 agtgagtccg gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg
    34621 atttttttct taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa
    34681 gtgatagatt ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa
    34741 ttgacgatcg tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct
    34801 tgtaaagcta atatcccagt gaaactcaaa caaatcagct gaaatatctg aaaactagcc
    34861 cagctgactg atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt
    34921 tgagtaaaat gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca
    34981 atcggtgcta attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg
    35041 aagtctgagt tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga
    35101 ggaacagtca ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa
    35161 gctacgaata aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt
    35221 tggatagctt ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt
    35281 tgataaggaa tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct
    35341 acttgacgga tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga
    35401 ccaatcagac aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct
    35461 ttcgatgaag ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga
    35521 tgtttgataa taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga
    35581 tgcagcaatg tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc
    35641 tccataatct tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg
    35701 aacataaatc tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat
    35761 ataatgcagt accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt
    35821 tccattttcc atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc
    35881 caaggcacct cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc
    35941 acaatctaaa atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa
    36001 ctgatagtta tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt
    36061 ttctgcatga gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt
    36121 aaaattttct tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc
    36181 ttgataggca gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac
    36241 ttcttgattt ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt
    36301 tttttggaag tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag
    36361 ttgttcagga aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac
    36421 tttttgaacg atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt
    36481 taatggaaac atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt
    36541 ttatcaattc aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat
    36601 tgtcttttag tccttctggg taatttttac aagtatcagg cataagatgc aggtagaatt
    36661 tttttgtctc ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta
    36721 aaagaggaat atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta
    36781 tatctggatt caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga
    36841 ccatttggac gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc
    36901 taccgaaggg aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta
    36961 caaataagaa atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga
    37021 taaatgattt ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa
    37081 aataagcaga gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg
    37141 ccgcagtaga atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt
    37201 ttataggcaa tgaagcagaa cagcaagaga agaagacgga gcaa
//
LOCUS       DAZUMI010000014        35554 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000014 DAZUMI010000000
VERSION     DAZUMI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.014"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..2000
                     /locus_tag="ACG81C_000570"
     CDS             234..2000
                     /locus_tag="ACG81C_000570"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5063757.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2102..3361
                     /locus_tag="ACG81C_000571"
     CDS             2102..3361
                     /locus_tag="ACG81C_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5063758.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3508..4809
                     /locus_tag="ACG81C_000572"
     CDS             3508..4809
                     /locus_tag="ACG81C_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5063759.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4812..5663
                     /locus_tag="ACG81C_000573"
     CDS             4812..5663
                     /locus_tag="ACG81C_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5063760.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5676..6491
                     /locus_tag="ACG81C_000574"
     CDS             5676..6491
                     /locus_tag="ACG81C_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5063761.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6642..7808
                     /locus_tag="ACG81C_000575"
     CDS             6642..7808
                     /locus_tag="ACG81C_000575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5063762.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8038..8685
                     /locus_tag="ACG81C_000576"
     CDS             8038..8685
                     /locus_tag="ACG81C_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5063763.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8769..9713
                     /locus_tag="ACG81C_000577"
     CDS             8769..9713
                     /locus_tag="ACG81C_000577"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5063764.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9848..11299
                     /locus_tag="ACG81C_000578"
     CDS             9848..11299
                     /locus_tag="ACG81C_000578"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5063765.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11320..12156
                     /locus_tag="ACG81C_000579"
     CDS             11320..12156
                     /locus_tag="ACG81C_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5063766.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12156..12815
                     /locus_tag="ACG81C_000580"
     CDS             12156..12815
                     /locus_tag="ACG81C_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5063767.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12941..13630
                     /locus_tag="ACG81C_000581"
     CDS             12941..13630
                     /locus_tag="ACG81C_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063768.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13636..14592
                     /locus_tag="ACG81C_000582"
     CDS             13636..14592
                     /locus_tag="ACG81C_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5063769.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14636..15148
                     /locus_tag="ACG81C_000583"
     CDS             14636..15148
                     /locus_tag="ACG81C_000583"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063770.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15404..15952)
                     /locus_tag="ACG81C_000584"
     CDS             complement(15404..15952)
                     /locus_tag="ACG81C_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5063771.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16041..17222)
                     /locus_tag="ACG81C_000585"
     CDS             complement(16041..17222)
                     /locus_tag="ACG81C_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS5063772.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17458..18966)
                     /locus_tag="ACG81C_000586"
     CDS             complement(17458..18966)
                     /locus_tag="ACG81C_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5063773.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19125..19316
                     /locus_tag="ACG81C_000587"
     CDS             19125..19316
                     /locus_tag="ACG81C_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063774.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19519..20436
                     /locus_tag="ACG81C_000588"
     CDS             19519..20436
                     /locus_tag="ACG81C_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5063775.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20429..21502
                     /locus_tag="ACG81C_000589"
     CDS             20429..21502
                     /locus_tag="ACG81C_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063776.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21650..22168
                     /locus_tag="ACG81C_000590"
     CDS             21650..22168
                     /locus_tag="ACG81C_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063777.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22149..22541
                     /locus_tag="ACG81C_000591"
     CDS             22149..22541
                     /locus_tag="ACG81C_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5063778.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22617..23552
                     /locus_tag="ACG81C_000592"
     CDS             22617..23552
                     /locus_tag="ACG81C_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5063779.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23685..24464
                     /locus_tag="ACG81C_000593"
     CDS             23685..24464
                     /locus_tag="ACG81C_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5063780.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24457..24774
                     /locus_tag="ACG81C_000594"
     CDS             24457..24774
                     /locus_tag="ACG81C_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5063781.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25018..26628
                     /locus_tag="ACG81C_000595"
     CDS             25018..26628
                     /locus_tag="ACG81C_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5063782.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26785..27633
                     /locus_tag="ACG81C_000596"
     CDS             26785..27633
                     /locus_tag="ACG81C_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5063783.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27864..28343
                     /locus_tag="ACG81C_000597"
     CDS             27864..28343
                     /locus_tag="ACG81C_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5063784.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28376..30604
                     /locus_tag="ACG81C_000598"
     CDS             28376..30604
                     /locus_tag="ACG81C_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5063785.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30733..32376
                     /locus_tag="ACG81C_000599"
     CDS             30733..32376
                     /locus_tag="ACG81C_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5063786.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32449..32994)
                     /locus_tag="ACG81C_000600"
     CDS             complement(32449..32994)
                     /locus_tag="ACG81C_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5063787.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33017..33502)
                     /locus_tag="ACG81C_000601"
     CDS             complement(33017..33502)
                     /locus_tag="ACG81C_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5063788.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33492..34304)
                     /locus_tag="ACG81C_000602"
     CDS             complement(33492..34304)
                     /locus_tag="ACG81C_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5063789.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34443..>35554)
                     /locus_tag="ACG81C_000603"
     CDS             complement(34443..>35554)
                     /locus_tag="ACG81C_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5063790.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11632 a         6397 c         7110 g        10415 t
ORIGIN      
        1 cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag
       61 tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa
      121 aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg
      181 cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata
      241 cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat
      301 ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag
      361 atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc
      421 taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac
      481 agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa
      541 tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt
      601 ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa
      661 tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt
      721 ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg
      781 atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta
      841 aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg
      901 gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca
      961 tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca
     1021 aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt
     1081 cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt
     1141 ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg
     1201 atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta
     1261 atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag
     1321 acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat
     1381 tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga
     1441 aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc
     1501 aggtgaacga aggaacattc aacttattag attctcatga tactgccagg atcttgaccc
     1561 tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag
     1621 ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt
     1681 gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa
     1741 aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt
     1801 ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga
     1861 tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg
     1921 tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg
     1981 gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga
     2041 agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa
     2101 gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg
     2161 cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa
     2221 aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac
     2281 tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt
     2341 ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata
     2401 tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga
     2461 agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga
     2521 acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc
     2581 atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga
     2641 agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt
     2701 aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg
     2761 tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt
     2821 ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat
     2881 gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa
     2941 caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc
     3001 gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca
     3061 aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa
     3121 tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac
     3181 aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg
     3241 cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc
     3301 aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg
     3361 attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg
     3421 caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg
     3481 tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa
     3541 aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct
     3601 tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc
     3661 ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga agatcattca
     3721 ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt
     3781 tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa
     3841 atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta
     3901 ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca
     3961 ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag tttaattgat
     4021 tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt
     4081 tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc
     4141 cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc
     4201 ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg
     4261 aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag
     4321 cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta
     4381 gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga
     4441 atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca
     4501 tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt
     4561 attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc
     4621 ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat
     4681 aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc
     4741 tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg
     4801 ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact
     4861 tttttactta cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca
     4921 cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg
     4981 actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata
     5041 aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg
     5101 cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt
     5161 tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct
     5221 tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc
     5281 caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat
     5341 ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg
     5401 tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca
     5461 tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa
     5521 catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga
     5581 ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga
     5641 catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt
     5701 tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta
     5761 aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt
     5821 atgccaatca gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg
     5881 atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat
     5941 cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac
     6001 aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa
     6061 aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt
     6121 ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc
     6181 gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga
     6241 gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat
     6301 ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca
     6361 tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa
     6421 caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc
     6481 gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata
     6541 aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt
     6601 agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat
     6661 acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta
     6721 caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag
     6781 aaatcccaat gaaagagatt acaagtaacg cttctttggc actaggagaa gcctatatgg
     6841 atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag
     6901 caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata
     6961 cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata
     7021 aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata
     7081 cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag
     7141 aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca
     7201 aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta
     7261 ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc
     7321 gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag
     7381 gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa
     7441 cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata
     7501 aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa
     7561 aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag
     7621 aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt
     7681 ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta
     7741 atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc
     7801 gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg
     7861 atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct
     7921 ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat
     7981 tttttcttcg ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg
     8041 aaaaaaactg ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca
     8101 aataccaatc aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag
     8161 gtatcggact cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt
     8221 gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa
     8281 gataaatacc cagatactgc aattactagt ttggaattag atagtgattg gggatcttac
     8341 ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa
     8401 aatgaaaaaa tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa
     8461 aagaaaaaag aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa
     8521 atcgccgtcg acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta
     8581 ggtactactt attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc
     8641 aatagccaaa caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa
     8701 acaagagttg atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag
     8761 cgatttactt gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga
     8821 atgcatctgg tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag
     8881 caggagcatt tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac
     8941 gctattttga tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt
     9001 cttattatct agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt
     9061 tatttttttc cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag
     9121 aaaaaagtgg gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa
     9181 aaccacaact agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca
     9241 tcttttctaa gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc
     9301 agatggctga tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg
     9361 gcaatggttt gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt
     9421 tcggcggcat tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt
     9481 atacacgttt gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag
     9541 atgcatttga acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata
     9601 aagaagggcc ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa
     9661 aaggatatac gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag
     9721 acgaaggtca tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg
     9781 tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga
     9841 ggataatatg aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt
     9901 tcaagaacta gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc
     9961 gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga
    10021 aaaagggcca ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg
    10081 atggctagct acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa
    10141 ttatgtgcgg caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga
    10201 gtttggtttc aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa
    10261 ccttgaatgt acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat
    10321 cgttccttta gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg
    10381 cggattgatt gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag
    10441 aggatggcaa ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc
    10501 tttattttat gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga
    10561 gtggcaaaca catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat
    10621 ccactcacag attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg
    10681 aaaagaaggg catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac
    10741 gtcttattat cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg
    10801 catccagcga gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata
    10861 tggtcagaag ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc
    10921 aattgtcatg gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt
    10981 taccgcccct aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca
    11041 catcagtgat tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag
    11101 cttgaagatc acaccttata cacagttggc taatctgact ggacaaccag caatcagctt
    11161 gcctactcat ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg
    11221 aagagaagat ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt
    11281 accaaatcca tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt
    11341 gctctaataa ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag
    11401 gaagataaca gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc
    11461 acactgtttc ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat
    11521 ttggcaaaag aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg
    11581 gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt
    11641 atcacaaaag aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac
    11701 agtatgatcg tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg
    11761 aaaaaagcag ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct
    11821 aaattagtca catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt
    11881 cgaacagatg ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca
    11941 gatatccctg taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta
    12001 ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa
    12061 gaagatggga cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa
    12121 ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt
    12181 ccctcatgtg gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg
    12241 gcttcgtgtc gtttctttta ggaaatctgg tagggttgct tttgacagtt ttaggtcttt
    12301 tggattggct gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc
    12361 ccgcactagt cctgctgttt ctgttatatt ttgggttacc ttatcaatta agtgccttaa
    12421 cggcttcagt catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag
    12481 gatcactagc aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt
    12541 tctttggggt attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac
    12601 tcagtaacgt agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc
    12661 cagaaatatt tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct
    12721 atattttagt tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt
    12781 atttagaaaa acgttctatg catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc
    12841 tggcaccatt tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca
    12901 tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat
    12961 cgaaatagaa tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga
    13021 agtcacgatg cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga
    13081 tggaagccga gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac
    13141 agccaaactg tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga
    13201 agaagtaaaa actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt
    13261 tgaagaggag atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga
    13321 gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt
    13381 aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt ccaacagatc aattgtacaa
    13441 agaattgaaa agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg
    13501 gtttttagag gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct
    13561 tcagcatgat tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc
    13621 attggagtga aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca
    13681 ttaaaagaag cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca
    13741 gtacgtgatt ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca
    13801 atcgctgtga tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac
    13861 agttggacag tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat
    13921 ctcagtgaag tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca
    13981 aaagatacag aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat
    14041 actagtcgct ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa
    14101 acgagaaagc atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat
    14161 ctttttttcc acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt
    14221 tatttcgata aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct
    14281 tacggcaata tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa
    14341 gcattgaagt ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat
    14401 caactcttga ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc
    14461 cctggactgg gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac
    14521 ctgctagatc atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa
    14581 gtgatccaat gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta
    14641 tattcggcaa acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc
    14701 ggatttcatt gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga
    14761 aacgattttg aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat
    14821 tgctggaaca gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag
    14881 ttggaaagaa tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac
    14941 tcgaggaaaa aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa
    15001 aaatatccaa gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat
    15061 ctattctgct ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag
    15121 aaaagcatat caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat
    15181 taacactact gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc
    15241 ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt
    15301 attttacgaa atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt
    15361 gtcacgacct ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt
    15421 tttttatttt atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg
    15481 ggatagcttt caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg
    15541 aaatctgttc agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga
    15601 tcgtaataaa gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat
    15661 ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac
    15721 tggaaccata tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca
    15781 tctgattgaa tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc
    15841 ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga
    15901 gtgaagtttt tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt
    15961 ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag
    16021 taactttgct tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg
    16081 actaaagtcg ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct
    16141 tcatttaatc ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa
    16201 acgaggaaga cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct
    16261 aattctgtca tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca
    16321 tccactgctt ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct
    16381 aggatctctt ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc
    16441 aaagcaacaa aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga
    16501 atgtattctc ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca
    16561 tcttcttttt cttctaatac atagggattt acaattgaag ccagaatata accgccaaat
    16621 aagttcaaaa caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg
    16681 acgatcgaca tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca
    16741 gataggaagg gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca
    16801 gaagctactc cattataaga ttccaatttg cccaacccgt tgattttact taatcctaga
    16861 ccaacagctt tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca
    16921 gaaataaata caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct
    16981 acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt
    17041 tttgccaata cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga
    17101 acaagctgta aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg
    17161 ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc
    17221 aaataatcac tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta
    17281 atctttagtg aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa
    17341 ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa
    17401 acgaaactat cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta
    17461 ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt
    17521 tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc
    17581 aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga
    17641 taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga
    17701 tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat
    17761 atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact
    17821 tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc
    17881 ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt
    17941 tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc
    18001 ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc
    18061 ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac
    18121 acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg
    18181 tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc
    18241 atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata
    18301 agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga
    18361 gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagg aagtagcgag
    18421 ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata
    18481 ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc
    18541 gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt
    18601 cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc
    18661 acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg
    18721 gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt
    18781 caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc
    18841 atgcttgtcg tcaactagct tcccaccaaa ttctaaatat aatttgtcat agtggttcac
    18901 tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt
    18961 tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca
    19021 actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga
    19081 tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga
    19141 aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc
    19201 ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa
    19261 ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa
    19321 taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa
    19381 aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt
    19441 ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag
    19501 taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa
    19561 ctagaggaga cgaatgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc
    19621 aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa
    19681 gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa
    19741 gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc
    19801 catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg
    19861 atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca
    19921 agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa
    19981 ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct
    20041 cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat
    20101 ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta
    20161 gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg
    20221 cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt
    20281 cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag
    20341 ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat
    20401 aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa
    20461 aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag
    20521 taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa
    20581 agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac
    20641 tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat
    20701 tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga
    20761 ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac
    20821 gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga
    20881 tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat
    20941 caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga
    21001 tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat
    21061 caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt
    21121 ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag cccatgcaag
    21181 aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa
    21241 tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat
    21301 gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga
    21361 taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc
    21421 atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc
    21481 attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt
    21541 ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt
    21601 actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa
    21661 taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca
    21721 atttattccg aatttccagc caatgactgc tattttttta tttatagtac tttacttaag
    21781 cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg
    21841 tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc
    21901 aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt
    21961 tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt
    22021 gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg
    22081 caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg
    22141 aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt
    22201 tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa
    22261 catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag
    22321 tgttcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag
    22381 aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct
    22441 attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga
    22501 atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta
    22561 aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg
    22621 aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag
    22681 ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa
    22741 gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag
    22801 ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca
    22861 gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt
    22921 ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac
    22981 tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag
    23041 aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa
    23101 tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag
    23161 gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga
    23221 tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca
    23281 tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg
    23341 agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg
    23401 cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata
    23461 ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag
    23521 ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa
    23581 tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac
    23641 aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa
    23701 gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag
    23761 taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac
    23821 tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga
    23881 cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta cgctatacat
    23941 tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct
    24001 tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc
    24061 cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg
    24121 gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc
    24181 attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac
    24241 tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa
    24301 atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga
    24361 aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc
    24421 cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt
    24481 ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt
    24541 actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta
    24601 tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg
    24661 aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg
    24721 gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt
    24781 ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc
    24841 atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg
    24901 gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat
    24961 tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg
    25021 aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt
    25081 ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg
    25141 cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt
    25201 acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat
    25261 aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc
    25321 gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct
    25381 aacgttaata atgggatact agctatcaga agtttatgcc catctttgat tgctatccca
    25441 attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact
    25501 tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg
    25561 cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc
    25621 ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc
    25681 gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct
    25741 gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt
    25801 caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca
    25861 gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg
    25921 atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg
    25981 aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta
    26041 ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact atacacattg
    26101 ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg
    26161 tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt
    26221 gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta
    26281 ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt
    26341 gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc
    26401 aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt
    26461 ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt
    26521 gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg
    26581 tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg
    26641 ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt
    26701 tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt
    26761 ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat
    26821 ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg
    26881 gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta
    26941 aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta
    27001 tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg
    27061 atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc
    27121 attccttatg actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta
    27181 gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga
    27241 gataatacct acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt
    27301 ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt
    27361 tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga
    27421 gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg
    27481 atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat
    27541 ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa
    27601 aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa
    27661 aatattccaa catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag
    27721 atggtgtttt tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc
    27781 tataatgtcg catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa
    27841 taaaaggatg aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa
    27901 tcgttgtaga agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag
    27961 ggctttcact gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg
    28021 tatggcgaca tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta
    28081 ttttagattt agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt
    28141 atgaaaaatt ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc
    28201 gaatgacggt agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt
    28261 acgatacact acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc
    28321 ttttagatga gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga
    28381 gaatttctca ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa
    28441 gaaaccgaaa agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg
    28501 gaattggcaa gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc
    28561 ggggaagaca caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt
    28621 gacctgtttt gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga
    28681 ttctaataag gaacaagacc aagaattact agaagctatt taccaaacag atacacttga
    28741 agctgtactg acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa
    28801 attagcagag ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta
    28861 ttacccttct gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc
    28921 gactgtcatc cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg
    28981 gatgttgaat ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac
    29041 tacaaatcga aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca
    29101 acatgcggaa aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa
    29161 aactatgatc caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga
    29221 tgaaactgca aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg
    29281 ggcaaatacc tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta
    29341 taaattatcc actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg
    29401 attgctactg gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga
    29461 gcatacaaaa ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa
    29521 tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga
    29581 gggagaagag aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa
    29641 agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc
    29701 tcaagaaatg tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa
    29761 taaagaagcg cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca
    29821 ccgaccagct ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc
    29881 aatagtagaa tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac
    29941 gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga
    30001 tttgagaaat tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt
    30061 acggatcgat gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc
    30121 agcgattgcc attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga
    30181 tacagcagac cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga
    30241 cggatttcaa tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga
    30301 tggtgctgca cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact
    30361 gttgcttaat attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc
    30421 aaaagaagat atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc
    30481 cgcagctatt ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt
    30541 agatattact gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct
    30601 atgataaaat aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt
    30661 taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag
    30721 gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc
    30781 tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt
    30841 gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat
    30901 caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga
    30961 ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa
    31021 gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg
    31081 aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc
    31141 aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa
    31201 actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc
    31261 gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa agggatagat
    31321 ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca
    31381 gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg
    31441 aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca
    31501 gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat
    31561 catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt
    31621 gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa
    31681 aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct
    31741 atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat
    31801 caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca
    31861 gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca
    31921 ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg
    31981 gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa
    32041 acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac
    32101 atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt
    32161 catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt
    32221 atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc
    32281 tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca
    32341 ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag
    32401 ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc
    32461 tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag
    32521 ggttttcaaa cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt
    32581 gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa
    32641 ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac
    32701 aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg
    32761 ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg
    32821 gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc
    32881 gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat
    32941 gtcattaact atttcagtca attggtcttt taattctttt tctgacaact tcattttcta
    33001 aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta
    33061 taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca
    33121 ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta
    33181 agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag
    33241 caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg
    33301 aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat
    33361 aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg
    33421 gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga
    33481 tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca
    33541 cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta
    33601 ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa
    33661 gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc
    33721 tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg
    33781 cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt
    33841 tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc
    33901 gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg
    33961 atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt
    34021 tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt
    34081 ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc
    34141 aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag
    34201 gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca
    34261 ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg
    34321 aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc
    34381 atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat
    34441 agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta
    34501 gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca
    34561 tggacttgct cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc
    34621 ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa
    34681 tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc
    34741 cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac
    34801 caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt
    34861 cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa
    34921 tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt
    34981 gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca
    35041 tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc
    35101 tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga
    35161 tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt
    35221 ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc
    35281 ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga
    35341 gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat
    35401 ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta
    35461 ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac
    35521 aaagcaggtt gttccattaa ataaagaata ggaa
//
LOCUS       DAZUMI010000015        34957 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000015 DAZUMI010000000
VERSION     DAZUMI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.015"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG81C_000604"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG81C_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5063791.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACG81C_000605"
     CDS             complement(2017..2919)
                     /locus_tag="ACG81C_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5063792.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACG81C_000606"
     CDS             complement(2916..3608)
                     /locus_tag="ACG81C_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5063793.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACG81C_000607"
     CDS             complement(3651..4292)
                     /locus_tag="ACG81C_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5063794.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="ACG81C_000608"
     CDS             complement(4330..4644)
                     /locus_tag="ACG81C_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063795.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG81C_000609"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG81C_000609"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS5063796.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG81C_000610"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG81C_000610"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5063797.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="ACG81C_000611"
     CDS             complement(5981..6913)
                     /locus_tag="ACG81C_000611"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5063798.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="ACG81C_000612"
     CDS             complement(6910..7686)
                     /locus_tag="ACG81C_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5063799.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG81C_000613"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG81C_000613"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5063800.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="ACG81C_000614"
     CDS             complement(10930..12006)
                     /locus_tag="ACG81C_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5063801.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="ACG81C_000615"
     CDS             complement(12011..13291)
                     /locus_tag="ACG81C_000615"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5063802.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="ACG81C_000616"
     CDS             complement(13310..14236)
                     /locus_tag="ACG81C_000616"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5063803.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="ACG81C_000617"
     CDS             complement(14249..15532)
                     /locus_tag="ACG81C_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5063804.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG81C_000618"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG81C_000618"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5063805.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="ACG81C_000619"
     CDS             complement(16320..17231)
                     /locus_tag="ACG81C_000619"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5063806.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG81C_000620"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG81C_000620"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5063807.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="ACG81C_000621"
     CDS             complement(17697..18395)
                     /locus_tag="ACG81C_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5063808.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="ACG81C_000622"
     CDS             complement(18382..18768)
                     /locus_tag="ACG81C_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063809.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="ACG81C_000623"
     CDS             complement(18915..20585)
                     /locus_tag="ACG81C_000623"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5063810.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="ACG81C_000624"
     CDS             20768..21001
                     /locus_tag="ACG81C_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5063811.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="ACG81C_000625"
     CDS             complement(21100..21531)
                     /locus_tag="ACG81C_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5063812.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="ACG81C_000626"
     CDS             21610..22023
                     /locus_tag="ACG81C_000626"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5063813.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="ACG81C_000627"
     CDS             complement(22061..23209)
                     /locus_tag="ACG81C_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5063814.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="ACG81C_000628"
     CDS             complement(23331..23927)
                     /locus_tag="ACG81C_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5063815.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG81C_000629"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG81C_000629"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5063816.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="ACG81C_000630"
     CDS             27157..27588
                     /locus_tag="ACG81C_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5063817.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="ACG81C_000631"
     CDS             complement(27633..28982)
                     /locus_tag="ACG81C_000631"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5063818.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="ACG81C_000632"
     CDS             complement(29052..30215)
                     /locus_tag="ACG81C_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5063819.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="ACG81C_000633"
     CDS             30344..30667
                     /locus_tag="ACG81C_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063820.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="ACG81C_000634"
     CDS             complement(30850..32199)
                     /locus_tag="ACG81C_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5063821.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="ACG81C_000635"
     CDS             complement(32268..33221)
                     /locus_tag="ACG81C_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5063822.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="ACG81C_000636"
     CDS             complement(33323..34513)
                     /locus_tag="ACG81C_000636"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5063823.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34713..>34957)
                     /locus_tag="ACG81C_000637"
     CDS             complement(34713..>34957)
                     /locus_tag="ACG81C_000637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063824.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9950 a         7640 c         6120 g        11247 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaaggac
    34921 tgtgacaaaa gtcttgtcac agtcctattc ttccgaa
//
LOCUS       DAZUMI010000016        34553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000016 DAZUMI010000000
VERSION     DAZUMI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.016"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG81C_000638"
     CDS             212..604
                     /locus_tag="ACG81C_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5063825.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG81C_000639"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG81C_000639"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5063826.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG81C_000640"
     CDS             2698..2943
                     /locus_tag="ACG81C_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063827.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG81C_000641"
     CDS             3165..4775
                     /locus_tag="ACG81C_000641"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5063828.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG81C_000642"
     CDS             complement(4752..4964)
                     /locus_tag="ACG81C_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063829.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG81C_000643"
     CDS             5067..5936
                     /locus_tag="ACG81C_000643"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5063830.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG81C_000644"
     CDS             6101..7372
                     /locus_tag="ACG81C_000644"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5063831.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG81C_000645"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG81C_000645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5063832.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG81C_000646"
     CDS             8763..9026
                     /locus_tag="ACG81C_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5063833.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG81C_000647"
     CDS             9471..9920
                     /locus_tag="ACG81C_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5063834.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG81C_000648"
     CDS             9977..11395
                     /locus_tag="ACG81C_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5063835.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG81C_000649"
     CDS             11395..12255
                     /locus_tag="ACG81C_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5063836.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG81C_000650"
     CDS             12429..13202
                     /locus_tag="ACG81C_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5063837.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG81C_000651"
     CDS             13349..13714
                     /locus_tag="ACG81C_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5063838.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG81C_000652"
     CDS             complement(13862..15070)
                     /locus_tag="ACG81C_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5063839.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG81C_000653"
     CDS             complement(15089..15367)
                     /locus_tag="ACG81C_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063840.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG81C_000654"
     CDS             15560..16216
                     /locus_tag="ACG81C_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5063841.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG81C_000655"
     CDS             complement(16658..17455)
                     /locus_tag="ACG81C_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5063842.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG81C_000656"
     CDS             complement(17452..18636)
                     /locus_tag="ACG81C_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5063843.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG81C_000657"
     CDS             complement(18661..19908)
                     /locus_tag="ACG81C_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5063844.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG81C_000658"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG81C_000658"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5063845.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG81C_000659"
     CDS             21888..22202
                     /locus_tag="ACG81C_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5063846.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG81C_000660"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG81C_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5063847.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG81C_000661"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG81C_000661"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5063848.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG81C_000662"
     CDS             23546..23875
                     /locus_tag="ACG81C_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5063849.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG81C_000663"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG81C_000663"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5063850.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG81C_000664"
     CDS             24863..25687
                     /locus_tag="ACG81C_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5063851.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG81C_000665"
     CDS             25680..26027
                     /locus_tag="ACG81C_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5063852.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG81C_000666"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG81C_000666"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5063853.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG81C_000667"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG81C_000667"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5063854.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG81C_000668"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG81C_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5063855.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG81C_000669"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG81C_000669"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5063856.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG81C_000670"
     CDS             31684..32451
                     /locus_tag="ACG81C_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063857.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG81C_000671"
     CDS             32441..34483
                     /locus_tag="ACG81C_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063858.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11111 a         6123 c         7256 g        10063 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgt
//
LOCUS       DAZUMI010000017        33559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000017 DAZUMI010000000
VERSION     DAZUMI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.017"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..372
                     /locus_tag="ACG81C_000672"
     CDS             <1..372
                     /locus_tag="ACG81C_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5063859.1"
                     /translation="NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDR
                     ELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNP
                     NMFVNYQNDPYQFTTQVILLF"
     gene            387..1139
                     /locus_tag="ACG81C_000673"
     CDS             387..1139
                     /locus_tag="ACG81C_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5063860.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1842..2057)
                     /locus_tag="ACG81C_000674"
     CDS             complement(1842..2057)
                     /locus_tag="ACG81C_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS5063861.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2076..2312)
                     /locus_tag="ACG81C_000675"
     CDS             complement(2076..2312)
                     /locus_tag="ACG81C_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS5063862.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2570..4723
                     /locus_tag="ACG81C_000676"
     CDS             2570..4723
                     /locus_tag="ACG81C_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS5063863.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4692..6107
                     /locus_tag="ACG81C_000677"
     CDS             4692..6107
                     /locus_tag="ACG81C_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS5063864.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6140..7174
                     /gene="sppA"
                     /locus_tag="ACG81C_000678"
     CDS             6140..7174
                     /gene="sppA"
                     /locus_tag="ACG81C_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5063865.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7176..7697
                     /locus_tag="ACG81C_000679"
     CDS             7176..7697
                     /locus_tag="ACG81C_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5063866.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7694..8080
                     /locus_tag="ACG81C_000680"
     CDS             7694..8080
                     /locus_tag="ACG81C_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5063867.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8576..8648)
                     /locus_tag="ACG81C_000681"
     tRNA            complement(8576..8648)
                     /locus_tag="ACG81C_000681"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8613..8615),aa:Lys,seq:ctt)
     gene            9029..9598
                     /locus_tag="ACG81C_000682"
     CDS             9029..9598
                     /locus_tag="ACG81C_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5063868.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9585..10445
                     /locus_tag="ACG81C_000683"
     CDS             9585..10445
                     /locus_tag="ACG81C_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5063869.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10635..11339
                     /gene="yycF"
                     /locus_tag="ACG81C_000684"
     CDS             10635..11339
                     /gene="yycF"
                     /locus_tag="ACG81C_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5063870.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FFNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            11344..13179
                     /gene="walK"
                     /locus_tag="ACG81C_000685"
     CDS             11344..13179
                     /gene="walK"
                     /locus_tag="ACG81C_000685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5063871.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            13176..14492
                     /gene="yycH"
                     /locus_tag="ACG81C_000686"
     CDS             13176..14492
                     /gene="yycH"
                     /locus_tag="ACG81C_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5063872.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14493..15362
                     /locus_tag="ACG81C_000687"
     CDS             14493..15362
                     /locus_tag="ACG81C_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5063873.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            15417..16226
                     /locus_tag="ACG81C_000688"
     CDS             15417..16226
                     /locus_tag="ACG81C_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5063874.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(16329..16958)
                     /locus_tag="ACG81C_000689"
     CDS             complement(16329..16958)
                     /locus_tag="ACG81C_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5063875.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            17128..18420
                     /locus_tag="ACG81C_000690"
     CDS             17128..18420
                     /locus_tag="ACG81C_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5063876.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18496..19158)
                     /locus_tag="ACG81C_000691"
     CDS             complement(18496..19158)
                     /locus_tag="ACG81C_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5063877.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            19383..19667
                     /gene="groES"
                     /locus_tag="ACG81C_000692"
     CDS             19383..19667
                     /gene="groES"
                     /locus_tag="ACG81C_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5063878.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19718..21343
                     /gene="groL"
                     /locus_tag="ACG81C_000693"
     CDS             19718..21343
                     /gene="groL"
                     /locus_tag="ACG81C_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5063879.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(21432..22571)
                     /locus_tag="ACG81C_000694"
     CDS             complement(21432..22571)
                     /locus_tag="ACG81C_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5063880.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(22682..23266)
                     /locus_tag="ACG81C_000695"
     CDS             complement(22682..23266)
                     /locus_tag="ACG81C_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5063881.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(23393..23824)
                     /locus_tag="ACG81C_000696"
     CDS             complement(23393..23824)
                     /locus_tag="ACG81C_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5063882.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(23842..24162)
                     /locus_tag="ACG81C_000697"
     CDS             complement(23842..24162)
                     /locus_tag="ACG81C_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5063883.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            24456..24596
                     /locus_tag="ACG81C_000698"
     CDS             24456..24596
                     /locus_tag="ACG81C_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063884.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            24894..25151
                     /locus_tag="ACG81C_000699"
     CDS             24894..25151
                     /locus_tag="ACG81C_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS5063885.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(25122..25430)
                     /locus_tag="ACG81C_000700"
     CDS             complement(25122..25430)
                     /locus_tag="ACG81C_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063886.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            25508..26254
                     /locus_tag="ACG81C_000701"
     CDS             25508..26254
                     /locus_tag="ACG81C_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5063887.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            26269..26472
                     /locus_tag="ACG81C_000702"
     CDS             26269..26472
                     /locus_tag="ACG81C_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063888.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            26488..26826
                     /locus_tag="ACG81C_000703"
     CDS             26488..26826
                     /locus_tag="ACG81C_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS5063889.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            26813..26992
                     /locus_tag="ACG81C_000704"
     CDS             26813..26992
                     /locus_tag="ACG81C_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063890.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(27035..27505)
                     /locus_tag="ACG81C_000705"
     CDS             complement(27035..27505)
                     /locus_tag="ACG81C_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063891.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            27592..28290
                     /locus_tag="ACG81C_000706"
     CDS             27592..28290
                     /locus_tag="ACG81C_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5063892.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            28468..28809
                     /locus_tag="ACG81C_000707"
     CDS             28468..28809
                     /locus_tag="ACG81C_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063893.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            28802..29473
                     /locus_tag="ACG81C_000708"
     CDS             28802..29473
                     /locus_tag="ACG81C_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5063894.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            29479..30171
                     /locus_tag="ACG81C_000709"
     CDS             29479..30171
                     /locus_tag="ACG81C_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5063895.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            30168..30917
                     /locus_tag="ACG81C_000710"
     CDS             30168..30917
                     /locus_tag="ACG81C_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063896.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            30929..31198
                     /locus_tag="ACG81C_000711"
     CDS             30929..31198
                     /locus_tag="ACG81C_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063897.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            31204..31368
                     /locus_tag="ACG81C_000712"
     CDS             31204..31368
                     /locus_tag="ACG81C_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063898.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            31361..31660
                     /locus_tag="ACG81C_000713"
     CDS             31361..31660
                     /locus_tag="ACG81C_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063899.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            31660..31962
                     /locus_tag="ACG81C_000714"
     CDS             31660..31962
                     /locus_tag="ACG81C_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS5063900.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            32002..32367
                     /locus_tag="ACG81C_000715"
     CDS             32002..32367
                     /locus_tag="ACG81C_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063901.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            32857..32997
                     /locus_tag="ACG81C_000716"
     CDS             32857..32997
                     /locus_tag="ACG81C_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063902.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            32994..33176
                     /locus_tag="ACG81C_000717"
     CDS             32994..33176
                     /locus_tag="ACG81C_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063903.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            33189..33383
                     /locus_tag="ACG81C_000718"
     CDS             33189..33383
                     /locus_tag="ACG81C_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063904.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT        11538 a         5400 c         6656 g         9965 t
ORIGIN      
        1 aataagattt gttgtaaatt cgaatgtcca agtactatta ttgaagttcc tgttccaata
       61 tttgactgta ttagggtgct tggaattatt ctcgataatg caattgaagc atctatagag
      121 agtcaagata gagaactgtc tctagctatt taccgagatg ataaacagat agaaatttcg
      181 atatcgaata gttgtagaga gatactactc tctatagata ctctaatgaa aaaaggaata
      241 acaacaaaaa agggacatca agggttaggt ttatctagta tagaggaaat caataaaaag
      301 aatcccaata tgtttgtgaa ctatcaaaac gatccgtatc aatttactac tcaagttatt
      361 ttgttgtttt gagggaagga ggatttatga gttatccaat aatattatgt gaagatcaaa
      421 ttatacaatt aaatcagtta gaacgaatta ttgataattt cattcttttt cacgataaag
      481 tatttaagat agtattgaaa acacaaagtc cgttagaagt taaaaaatac ctcaaacaat
      541 tccgaccaaa gcaaggaata tatttcttag atattgattt aaatcatgaa gttaacggta
      601 tagaattagc agaagtaatc agaaaatatg atgttcaagc aaaaatcatt tttacaacta
      661 ctcatgatga gatgttaccc gtaacaataa aaagaagagt tgaaacgtta ggatttgtaa
      721 caaaagatca aacactagat gagtatcgaa acgagattgt tgagttattg ttattagcgc
      781 aagaaaggat agatgcaaca aaacaggtta cgaatcaagc atttgttttt tccataggtt
      841 ctcaaacatt tacttttgat ataaatgaca tttattttgt agaagcttca aaattaccac
      901 atagattatc attgtgtact aaagatgggc agtacgaatt ttatggaaga attagtgagc
      961 ttgaaaagaa atatcctatg ttaacaagaa ttagtcgagc gtgtttagta aatatcttta
     1021 acgttaaaga aatagatttt aaaaaacgtt cactatattt tgattcagag ttggccagaa
     1081 actttacttt agggaaagca caaaaaatta aggagaagtt gaaagaaagc actgtttagt
     1141 ggttttttct ttagggagat tgcatttttc taataaaata gattccttca ctagtgcaaa
     1201 tttttctgaa tcctattaaa gttgtaccaa tttttatagc tgttattagt tacgtgcata
     1261 acagcaatat tagttactgg aggaatattt ctatggtctg gaaccaaaaa agggccaaat
     1321 gaatagttct tttcttgttt ttattaagta ccataaaggg gaattaaaat taaatgtatg
     1381 gtggaagatg cgaaagattt ggcaaattct actaattttt agaaagtgtt tttcattttt
     1441 tagttaaaaa atttgtcctg tcaagtgcaa tcctatgatt gtacttgata ggatttgaga
     1501 taattacttt cctgttttta taaatttaac ttgcaggaac aggctttaat gcacatccga
     1561 caagtacatt accaatacct cctgtaacgg ccgaaatagg tcctcctgca gcaccaacta
     1621 cagcgctgca agtccttttg taaaaatatc tctaccatta catttccaaa gaatttttat
     1681 ctgactttaa taaaagataa aaatttaata taattataaa aactagcaca agaagcttta
     1741 gtaaaaagtt ctgtccattc caaacttttg aaaagataaa gaacatacct atgttcgtaa
     1801 tcgacacaaa ccaatcttga ggtttgatat tttttaattg tttacgcacc ggtccacatg
     1861 ctgaatccag cacctaataa agctccacct acaccacaag cagctattgc ataaggtcct
     1921 ccggctgaac aaagttttac tcctgctgtt gccccagaaa aagtagtgcc taaaactccc
     1981 acggcactcc caccttgaat atttttcaac tcttctgaag ataatataaa tgttgttttt
     2041 gtgtttttaa tatgcatact acttattctc cttttttatt tttttcgagc atcccagcca
     2101 gctgcagcac ctccagcgac agctccatat aaccctccaa caacaccgca agctggtccg
     2161 caaattgctg ttcctaaacc tgcaccacct actgcccctg tggtaagtcc acctacaact
     2221 tgccccaatt ttccccaagc attattgcct ccaccaacag tttgtgcatt tccacaatac
     2281 ttagttcctt tacattttgc atgtttaaac acgcttcttt aaataatatt ttttacggtg
     2341 ctactgtaac atacgtattt gattaacagt aaaaatcttc ctgaaactta tcaagtttat
     2401 tcttgaatag taaaatttcc aaaataaatt tgattcagga atgaaaaagt tagtgttcaa
     2461 gaactgtttt caaagttttt gtggaattat gagacaatat tttcactgta ttaaagctat
     2521 attgagaatt tttctataaa aattttttag aaggagttgt gttagataag tgataaaatc
     2581 atttcctttt gtgcagcaac aagatgaaaa agattgtggt gttgcttgcc tttcgatgat
     2641 tttaaaatat tttaaaactg atatcccgat tcataaatta cgggacctat ctgggactga
     2701 tttagagggt acttcagcat ttggattgaa aaagacattt gaaaaattgt cttttgattg
     2761 tatggctatt caagcagata gtgagatctg gaaagaaaga gatcttatct taccattaat
     2821 tgcacatata ttggttgata acagctatat gcactacgtt gtaatttata aaacgaaggg
     2881 aaacgtacta tggattgcag atcccgctaa tggaaaaaat agaaaaacga ttgaagcgtt
     2941 tcaaaaagaa tggacaggta ttcttttatt acctatgcct agagaaactt atattcctat
     3001 taaagaaaaa gtagctggat taagttcttt tttgcctata atttggaaac aaaaaggatt
     3061 agtattccac atcgttttag catcgatttt cattacgttt tttggcatag gaagctcata
     3121 ttattttcaa ggtattcttg attttttcat ccctaatcaa gcacgttcaa ctttaaatat
     3181 aatttccatt ggcttgatcg ctgtctattt atttcgggta ttgtttgaat atagtcgtag
     3241 ttatcttctt attattttag gacagcgaat gagtatggca attatgcttc agtatttcaa
     3301 acatgtgtta actttaccaa tgaatttttt tgctacgaga aaatcagggg aaattatttc
     3361 tcgttttctt gatgccagta agattattga tgctttagca agtgctacgt tatcaatatt
     3421 tttagatatt ggtatggtgt tgcttgttgg ggttacgtta gctattcaga atagtacgtt
     3481 gtttttaatc actctatgct ctttgccttt ttatgtagtt gcaatattcg ctttcgtgaa
     3541 aagctatgaa aaatcgaaca gggaagaaat ggcagccggt gcagctgtaa attctagtat
     3601 catagaaagt cttaaaggaa tagaaacaat taaagcatat agcggtgaag agaaagtata
     3661 taaccgtata gaccatgaat ttgttaaatt aatgaaaaaa tcatttcgta cggttacgct
     3721 tgataatatt caacaaggaa ttaaacattt gattcaatta attagcagtg cattaatttt
     3781 atggctaggt tcttattttg ttatggataa tagaattagt ctaggtcaac ttatcacata
     3841 caatgcttta ctagtatttt tgacagatcc tttacaaaac attattaatt tacaagtgaa
     3901 aatgcaaacg gctcaagttg caaataatcg tttaaatgaa attttcgcga ttgattcaga
     3961 aaaatcgaac cagtcttttg aatatatgct ttctgaacaa ttatttcaac aagatatcac
     4021 tgtaaaaaac gtttcattct cgtatagcat gaaggcccca acgttgaacg aaatttcatg
     4081 tacaattcct tcgcacagta aaacggctat agttggagtt agtggttctg gaaaatcaac
     4141 tcttgcaaag ttatttgttc gtttttatga agcatcagaa ggatcgatcc attatggaaa
     4201 cattaatcac ttagacattc cttttcagat attacgcgat cgagtgacat atttgcctca
     4261 agagtcattt ttctttagtg gaacaatcat tgagaatctt atattcggtt tagatagccc
     4321 accaagtttc gaacaaataa tagaagtttg tgagatagtc caactaaatg aatttattaa
     4381 tcaacagcca ttacgttacg agacattgtt ggaagaaggt ggaaataatc tttcaggagg
     4441 gcaacgacaa cgattagcct tagcacgggc tttattaaaa aatccggaca tattaatttt
     4501 agatgaagca actagtggat tagatacctt gttagaacat gcaattatga attatctcct
     4561 caatttaaaa gaaaaaacga ttatttttat tgcgcatcat ttaccaatag caaaagcatg
     4621 tgatcaaatt ttagttctcc atgaagggca tttggtagaa aaaggaacgc acgatgaact
     4681 tcgttttgcc gatggtatct atcaaaaatt atgggaaatc taggagaggg aaacaattta
     4741 tgcagaataa taattggttc gaacattcta gtatctatac taaacaacat catagcttct
     4801 accgttgggt gttataccct gtaattcttt ttttatgtat tattggattg tttttaacct
     4861 ttgctaaaaa agaggttgtt attcggacaa cggctcaatt gaaccccgaa aaaatagaga
     4921 agttacaagt gccattagaa gctaaaataa tggaaaactt cctaaaagaa aatcaatttg
     4981 ttcataagga cgatgtgttg gttagattgg attgttcttt aatagagaat gaaaaggctc
     5041 agatagaaca agaaaaccaa cgtattactc aacaaataaa gatggctcag ctatttattg
     5101 aaagtataag taaaggaaaa aatttgtttt caacggatga cagttttggc tacagtaatc
     5161 aattaaagag catgttgtca gaaaaagaat cactccgcta cgctttgaag caaagtgaat
     5221 taaatgatca aaagcaatta gaagtatacg aaaagacaaa aagacaacta gaaaaacaaa
     5281 ttgagagttc agatagtaaa ttacaagaat ggcaacaagt acaggtagct tggagtaata
     5341 atcaatcatt aaaagatttt tcaaaagaaa tgatggcaaa ctatgagaat tggcaagaac
     5401 aactaaataa tgtttctgat gatcaaaaaa atcaagtgaa actgacaatt tcagcaagca
     5461 taaatgaaca aattgagcaa ctaaaaaaag aagtagaaca gtatcagtca gaaaaagcta
     5521 aattagttaa accaactact tctgagaatg acagaattag tcaaacggaa aaaggaaagc
     5581 aagagctaga acaagcaata acaactgtaa aacaaacagt tacagagcta aatgaaaaac
     5641 aagagaagaa ccagtcagtt atcaaaagtt tggacgatca actttcaaaa ggaatactaa
     5701 aagctcctgt atctggaacg gttcatttaa atgaagaaac aaagggacaa gcagaactag
     5761 ccaaaggaac agtgcttgca gaaatttatc cgatccacga aaaatcaaaa atgaaattta
     5821 cagcactttt acctgcgaat gaaagtacct atattaaaga agggatgaag gttcatttta
     5881 aattggatca aaaaggtggg gctccgatta cgatcgacgg aactttagat gaaatttcag
     5941 aaaattctac tgccactgag aaaggtgttt tttatgcagt gaaaggaacg ttaaaaacaa
     6001 ctcaatcgtt tccgtttcgt tatggattaa ctggagagtt gtctttaatt gtaggagaaa
     6061 aaacatattg gcaacaaatt gtcgatgtgt ttctaaataa aaaataatta tttttactaa
     6121 gaatatagga ggagtttata tgaataaaag aagatggatt gctgtgggag tagcgtgcgg
     6181 attattgtta ctatccatta tcgtcgcgct aattccagga aaagataaag aggaagcatc
     6241 aaatacaacc ctaacaggaa taaataagat tttttacgga tctaacgaaa tcacagaaga
     6301 aacgttagaa gaaggcgcat caaataaaaa gattgtcaaa ctatccgtca atggagttat
     6361 tgcagataca ggtgaaagca atctttttag tagggaacaa tataaccatc agaatttttt
     6421 aacacagcta aagaaaattc aagaagacaa agcggttaag ggagtgttat tagaagtaaa
     6481 ttcacctggg ggaggaatat atgaaagtgc tgagattgct aaagaaatgg caaaaattaa
     6541 aaagctagat attcctatct atactgcatt taaaaataca gcggcaagtg gagggtacta
     6601 catttctgct ggttcagata aaatatttgc tacggaagaa acaacaactg gatcaattgg
     6661 tgtcattatt tctggtttaa attattctgg cttattagaa aaacttggcg taactgatgc
     6721 gacgtataaa agtggtgcat taaaagatat gatgtcacct caacacaaac cttcagagga
     6781 agagaataaa gttatacagg aatttgtgat gagtgcttat gatcgttttg tcaatgttgt
     6841 agcaaaagga agaaatatgg atactaatgc agtgaaagaa cttgcggatg gaagaattta
     6901 tgatggtaac caagcagtag aaaatgggtt ggtggatcaa attggttact ctgaagatgc
     6961 attagattcc ctaaaaaaag agaagaaatt aactgatgcg acggttatag aatataaaaa
     7021 tgacacaact ggctttgctt cttcttggtt aggtaataag attgctgaat ggcaagggct
     7081 aaaggcaact acatcagatc gtgtaatttc agtttttgaa aaacttggat tttctgatac
     7141 tccaaaacca atgtattact atggagggct ataaaatgaa taagaagagt ggcattgaaa
     7201 ctttgcctaa ttatgtattt gcgggatttt ggttacgatt ttttgctttt cttattgatt
     7261 taatttgtat tagcatcatc acccatgcta ctataggtct cttttacaaa atcatgggac
     7321 tggaatctga caaatccatt attacttttt atggtatagc gtcattagct atttatttaa
     7381 cctattttgt acttttgact aagctgaatt caggacaaac aattgggaaa atgatttttg
     7441 ggataagagt agtgagcttt gaggaaaaag agctaagttg gcaaactatc tttattcgtg
     7501 agtgtatatg taggtttata ttaaaaaatt ttgtatttca aattgggtat ttggtggctg
     7561 ctttcacgcc caagaaacaa catattgggg attatttttc taacacgtca gtggttgtta
     7621 ttcgttcatt agaagctatt aactatggtc aaaaaatttc taattctaaa acacttaatc
     7681 tggagagaat taaatgaata agagaaaaac agagtttatt ttgattttaa ttagtggaat
     7741 aacagggatt ttatgtagtc ttttagcatt gatgggtggt atgtttctat cttctattga
     7801 ttcaactact ttaaatacta cagaactaac cagtaaggat atagatatgt tagtcaatct
     7861 aagcgggaat gttgttttat ttggaggaat taccctagtt ttttcgataa tgattttcat
     7921 aataggtttt ttagtaagaa aaaatcaaaa aacagtggtc tatggtatta gtttactcat
     7981 tttatctatt attccatttt ttcttttaac attattgtgg gtaattcctg gagttttagg
     8041 tattattgcg ggattgatgc ttctaataag aaaatcttaa ttaggcaatg tgtagacaac
     8101 gtgaaacgat taatcaatta aaagctaaat acgttttgag aaatgataac ttgtagtcga
     8161 tggaagacat gtagataaaa aaactacgtg tcttttattt attctaattt tttcgaaaaa
     8221 aagacactct ctgattgttg tattaatgga aaaggggaaa gcagcaagta aatttagttt
     8281 ttctattaaa tattcttcac tttttaacct tttatagtac ttagatttaa tcctatatgt
     8341 ttagagagtt cattggcagc atgattttgc aaagcttctc gaattattgt tatattagaa
     8401 actttactgt tcataattat gcttcttttt taactcaaag tccaaagggc tacagtggac
     8461 tttgagttaa aaataaaaag ccctagttat tttgaaaaat aaaaaaagaa cagcgtaaat
     8521 gttttcgaaa tgctttcgca ccaacattta cctgttccgt gggtttggca cccaatgggc
     8581 cgtgaggggc tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct
     8641 aacggcccgt acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact
     8701 acatttaaag aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt
     8761 cacatttgct cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac
     8821 cctaacactt ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc
     8881 ttaacgataa aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct
     8941 aatcaaatca cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt
     9001 ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg
     9061 ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact
     9121 ttatatggac caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga
     9181 aaagcatcca ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc
     9241 agcaccagtt aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac
     9301 gttgaaacaa gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac
     9361 agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat
     9421 ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt
     9481 agaatatatt gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa
     9541 aacgattgtt ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa
     9601 tcgcaatttt agtagattca ggtacggatg ttccagaaga attgataaaa aaatacgaga
     9661 tgtatatgat cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca
     9721 ttacccctga agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg
     9781 atggtgagtc gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac
     9841 ttgcggtaac aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac
     9901 aacagcaaga agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag
     9961 gtttttctgc gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga
    10021 tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg
    10081 aatatcttca aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa
    10141 agcttaaccc aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta
    10201 ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg
    10261 caaaggtctt caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg
    10321 aagcgagact taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc
    10381 cggcattagt agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag
    10441 gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt
    10501 tagaacaaaa aataataaag tattttgcac tttcatttta attttctctt ttgttttata
    10561 ggcaaagatg aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata
    10621 agaggtggct ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata
    10681 tcgtcaaatt caatttagcc aaagaaggct atgacgttta tactgcatat gatggggaag
    10741 aagccttgga aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac
    10801 ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca
    10861 ttatggtaac agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg
    10921 atgattacgt gaccaagcca tttttcaacc gagaattagt tgcacgtgtc aaagcaaatc
    10981 ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga
    11041 ttggtgattt gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg
    11101 agttaacaca tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga
    11161 tgacaagaga acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa
    11221 cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa
    11281 attatttagt gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat
    11341 tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt
    11401 attctgattt tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa
    11461 cgttccacga tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca
    11521 acaatcagcc cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat
    11581 atcaaaagat ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac
    11641 gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa
    11701 aatgattatg ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac
    11761 gataaacggg tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc
    11821 ggggctcttt acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca
    11881 gtcattttct ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta
    11941 gttgctcgtt cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct
    12001 agaggagact acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct
    12061 gataccttca atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa
    12121 cgcaatcgtt tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc
    12181 aggggtaaag tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag
    12241 gaagcaatcg gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg
    12301 aaattattag aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt
    12361 gatcagatga tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc
    12421 ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt
    12481 gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat
    12541 atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag
    12601 gtaacgctag atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct
    12661 cgcatggata caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg
    12721 aattttgttc ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc
    12781 cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa
    12841 gtagtcgata atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact
    12901 tgtcgcttat tagaaacgca taataacgtg attttaagta tcaccgatca aggattgggg
    12961 attcctaaaa aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt
    13021 gcaagagcac aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg
    13081 catcgaggcg ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg
    13141 ctgccatatg aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc
    13201 gtataggact cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga
    13261 gcccagcagg aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc
    13321 aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg
    13381 gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata
    13441 aagcaagatt tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg
    13501 tgaaaaatct gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat
    13561 acaaggaagc atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata
    13621 tgtattttac tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt
    13681 cgaaccatct cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat
    13741 taacagcaaa agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc
    13801 aatatgctac gaaacattcg atcaaactga agaagtatag ttatatcctg tcacagcaac
    13861 cctatacact ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg
    13921 aaagcaatga attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc
    13981 aagtagttga ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg
    14041 aaagtttccc ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc
    14101 gcagtgacaa tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag
    14161 acagcagagg acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga
    14221 atatccaaac cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac
    14281 ttgcaagtac gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga
    14341 ttggttcagt gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg
    14401 atctcttacc tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc
    14461 tggaaaaatt acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat
    14521 ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat
    14581 accaagaaaa caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta
    14641 gcgacgatat cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta
    14701 gcgcggagca gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg
    14761 gcttgctggc aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt
    14821 cagatgctga aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt
    14881 tatctaacaa agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc
    14941 atttagaaga agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca
    15001 atgatgatac agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca
    15061 cgaaatacat gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt
    15121 atacggaaga ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa
    15181 aaattttatg gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt
    15241 atgttcctgt ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagcg
    15301 tcaacgcttt cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat
    15361 aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg
    15421 ctgaagaaaa accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac
    15481 tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa
    15541 tcacttcctt gatggctcaa attgatcgga caccggacca attagatgga atacttgtga
    15601 cgcatgaaca tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg
    15661 atgtttttgc caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag
    15721 tggaacaaaa acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg
    15781 agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa
    15841 atcggtcatt cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca
    15901 ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg
    15961 gaccttatcc gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg
    16021 aagacggtgc acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg
    16081 gccatcttag caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt
    16141 tgaaagaaaa agattttggc gttggacatg attttgaaat ctatgataca gatccagatg
    16201 aagcaactga gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag
    16261 ataaaatcaa tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa
    16321 taaccgtatt atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc
    16381 tcatctacta atgcaatcgc gtaatctgcc atgctgatcg tactgtcgct agcagcgttt
    16441 ctttgtaaat aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat
    16501 aaagaagctg gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat
    16561 ccttcgtaca tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc
    16621 atacgagtcg tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag
    16681 cgtgtatcgg ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct
    16741 tcttttcctt tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta
    16801 gttgtcaact caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga
    16861 tgacgcacga ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa
    16921 cctgcatggc cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc
    16981 tgtccattgt aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt
    17041 gcgcattcct ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat
    17101 aaaaatagaa gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca
    17161 atgataacat ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac
    17221 acgatcccaa taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat
    17281 tacatcgctc agtggataga caaaacagga ttttctatgt attttacaca agtataccag
    17341 gctacatttg atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa
    17401 gaagaagaca tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt
    17461 attcagccac taaatactca gatagatact gtctggacag gtgctgaagg gatactagga
    17521 gcagttacaa ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa
    17581 gggtggaagc aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt
    17641 ccaatcattg ttatagtcac ttttacgctt atcggttcag cggtttttca acatttgttc
    17701 agaatgactg ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata
    17761 gacacgttgt ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt
    17821 cttcatggat ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta
    17881 gcaaatgcag aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc
    17941 gttactcagc aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg
    18001 gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt
    18061 gctcttttac cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg
    18121 atgaacccga tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc
    18181 tattccgctt tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca
    18241 acaccgccga tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt
    18301 atcgtgttgt caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca
    18361 aactgcaaaa aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa
    18421 aaaacaaaaa tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag
    18481 tatttatagt catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa
    18541 tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc
    18601 taggctgaag aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa
    18661 cgcaaacagc aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat
    18721 ccccagtata ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc
    18781 caaaataaaa gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg
    18841 ggttgctttg ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc
    18901 gacaaagatt ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc
    18961 tgattcgatc gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc
    19021 tagtatatac atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat
    19081 cgaagctagg atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct
    19141 atattttttt aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc
    19201 ttatatcgat tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt
    19261 cttttctttg atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag
    19321 gttagcactc gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta
    19381 tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa
    19441 ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg
    19501 ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag
    19561 caggcgacca agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag
    19621 aatatctgat cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa
    19681 aaaaataatc aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc
    19741 agaagacgca cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt
    19801 aacattagga cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat
    19861 cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat
    19921 gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac
    19981 aacgacagca actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc
    20041 aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga
    20101 agaattgcat aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc
    20161 tgtttcttcg ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg
    20221 aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt
    20281 agaaggtatg cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa
    20341 aatggaagct gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat
    20401 ccaagatatt ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat
    20461 tgctgacgat gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac
    20521 attcaacgta gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga
    20581 agatatcgct attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa
    20641 agatgcaaca atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac
    20701 aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa
    20761 aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc
    20821 taaattagca ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa
    20881 agaattaaaa ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg
    20941 aatggtctca ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga
    21001 agcagaagga gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat
    21061 tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa
    21121 cgtggaacta ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc
    21181 aggtatcgta gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc
    21241 tgccttgtta ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc
    21301 agctcctgca atggatccat caatgatggg cggtatgatg taggaattaa aagagaaccc
    21361 ttatattaca gggttctctt ttcttttttt gttttatttt aagtaaaagg ggcaaaaaag
    21421 gggcagaaat atcaaagcct atttagttta tcaattactt gttgttttgc ttttttggtt
    21481 acgtgattgt aaatagacag agttgtattt gcatcagaat gtcctacacg ctccataatg
    21541 gctttaagag gtacgcctaa ttcagataat agcgaaacat ggctatgtct aaatatatgc
    21601 gatgatagat ttttttctaa ttccaactct tcttctactt tatggagtac agcgttaaat
    21661 gagtgcagcg taagcggagt gccatttgta gatataaata tatattgatc gggattggtg
    21721 ggtctacctg caagaatatt gtcagctatc acactttcaa tcaattcttt tgcacgattg
    21781 gataattgta cttcacgttg tgagtaagta ttttttggag ttgtttttat ggcgttatcc
    21841 atttttacag atgtgtaatc taaggtccca ttaatggaaa ttttaccctc tccatagtcc
    21901 ttcatttgca aagccagcaa ttccccatat ctcaaaccag tcaaatatag aaattcagct
    21961 attatgccgt gtagttttct gcgaggattg gaatataact gtttcagtat ttgatcaatt
    22021 tcttctttct ccagatattt tttatccata gaaagctttc ttttttcttc ttccactttt
    22081 ttaggatgga ttttaactgc tagagcaggg tttctttgaa tgtattttct atcaattgca
    22141 tagtttagca taacggatag agttgttttt gtttgctttg tgtagttcag tgagagatca
    22201 ccaaaagtgt acatatcttc aatcatctta ttaatgagtg tctcatcaat gtttctaacg
    22261 attgtatcat cgcttatgtg cttagaaaca tgtttcatca tcattggaac cttcaaatag
    22321 ctagtacgtt taacatgctg cttataatat tcataccatt ctttatacag ctcaccaaaa
    22381 gtgatatctg atttattgta attttcgagt gcttctttaa tttttttatc tagaattttc
    22441 tgagcttttt tccacgcttg tggtgaatta cttgtaagtg ttgtagattt ttttcgtgtt
    22501 ttttctgtat aaggatctac atatctttca ataaacttga attggccatc tttggtttgt
    22561 tcaacccaca cttttaacat ctcctatcat ttgctataat aggcataaca aatagaccta
    22621 tataggtttg ttttctaaaa gcacgctctt actctggacg gtggggcgtg ttttttattt
    22681 attatacagt tgctttaaaa atagctgctt tttcaaatga tactaaagga gagaagtgga
    22741 tttctatttc tcccttttgg tttaacgtga aatgagcagt tacatccatt tgttttcctg
    22801 gagcaacaga tcccattgta ttttcgttgg catatgtctc tgattttttg tcatctggtc
    22861 catatacttc cacatctgta cctacaggga tatctgaatc gccatcattt tttacggtat
    22921 aagtaatttt tactacttgt gcaggttggt tttcttcaaa ttgatttctt tcatcagtta
    22981 gttctacact atttagtgta tattcagcat caccaacttt tacagtgtca ccaatcttat
    23041 agaaggtatc gcttttttct tctttagaag atgaagcaga ggttgaagat ttagttactt
    23101 tttcgccacc attatcactt gctttattac tatctgatcc accatttaaa gcagaaccaa
    23161 taattataat taaaattact gctaataccc aaaaccatac ttttttgtaa aaaggtttac
    23221 taactttata catttttccg tcttgaccca taactttttt tgccatttaa atattcctca
    23281 cttcttgtta taatatattt gcgatctcag aaatgaggta tgagtccgtg tgccagcacg
    23341 ggctttttta atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat
    23401 attgccttgc gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt
    23461 atctgagctt attctgaata gaataatact ccataaattt ttcgagatta aactgagatt
    23521 catcagtcag ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg
    23581 cttctgtttc aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa
    23641 aatgagaggc gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcgc
    23701 catatagatt ttcattcaaa aaaatgattc taggcttcgg ataatagaag ccaggctctt
    23761 tcatttccat gtaaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat
    23821 acatacaacc catcactcca actattcatt ttcctctaaa gctttagcga ttgcaatcgc
    23881 tttacgcatt gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgttttc
    23941 tgataaatcc acatgtttag gggtttcttt ttcttcgcgc cccagaagat agtctacaga
    24001 gacatcgaaa tagtcagcaa tttcttgcaa acgttctgta gaggggtttg agttcttaag
    24061 tctatataag acgtttttac catagcctag gtcttcttca actttattta atgattttcc
    24121 ctgttttttc gctaactctt ttattctctc aaacgttatc atatcaatat tcctttcgtt
    24181 caagaaaaaa tatttaactt tatttgttaa aattcgttga ctattttaac aaaaggtgtt
    24241 aatatcattc ttgtaaacaa gtttaacaac taaaaagaca acaaaaaaca ctattgatta
    24301 ataaatgcca accgccaaga aagcttttaa aatcaatgtt tatatgtctt atttaactat
    24361 gttctgattt taacactgta tgttaattta gtcaacgcag ttttttaaaa agttgttaaa
    24421 tttgtttacg aatataaaag aaaggagaga agaatatgga aaaaacagtc tcggcaaaaa
    24481 tcgaagattt gaaattagac atactgaaac aagcaaaagt ggcgatggaa cacgcggtag
    24541 ataaagaaga ctctgccatg gttgcagcca tagcagagat tttagctcac gtttagtcat
    24601 cgtttttacg gtcttctgga agctgaccat agataacagc ataatgaata tctaaataag
    24661 tttgaacaat ttcttttggc gaaattgatt cgcctttagt agatacttgt gattcgtgat
    24721 ttagccatgc aacaactaaa tccgcagcga tttcaaacgg cgttacactt tgtgtcagtt
    24781 gccataaaga atttcacaat atgtatggtc gtggtaataa taccaaagag caatttgagg
    24841 aatttatgga aattaaaaaa tacaaaaaat catttattaa aggaggctaa acaatgaaca
    24901 aggaaatgag ccaaacaatc aaggtacaaa aaatgataga tgatctaaca catggaattg
    24961 atagccaagc cgataaaatc atcaaggaat tgcaaggaca aaaagttaag gatgcaaaaa
    25021 tgcttctcaa gactatcaac tttgaaatga atccaacaaa aagaaagctc gccgatgtgt
    25081 tggaagaaaa gttggcttcc gctatcaacg agcaagaatt actatttgaa accgatactt
    25141 ttaacagctg atgttttata gatgacagtt tcatccgaat ctatatcatc gccgagagaa
    25201 aagaatgttg cttttgacaa aaagccgata agacctacaa gcggtcttga tgttgcaaga
    25261 gtagataccc caatatcacc ttgcaaggta ttcacgtaaa tttgagaaga atagaacatt
    25321 ccatcttttt cttcgatatt tgacgcatca tcgtatccaa cgaccaaagt atcggcaaat
    25381 attttgattt tttctccatc aagcatagta attacaaatt ctggtttcat aaattttcac
    25441 ctccttaaca attatttcag cctgtcacac tgataaggaa attataccaa agaaaggaat
    25501 gagaaatatg aatacaccac aaatttttaa tttcgagcaa aacgaagttc gaactatttt
    25561 agtaaatgat gaaccatatt ttgtaggaaa agatgtagca gacgttctag gatattcgaa
    25621 tccgcaaaaa gctattcgag atcatgtgga cttggaggat aagacgcaga acgattcgtt
    25681 caccgtcaat ggaacagcag ttgttttgat taacgagtca ggcctttaca gtttaatctt
    25741 aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg gtaacgagtg aagtccttcc
    25801 aacaattaga aaaacaggta gctattcaaa cgtacctcaa agttttgcac aagcattgcg
    25861 tttagcagcg gatttagaag aaaagaacca attactcgaa caacaaattg ccgagtacga
    25921 accaaagatt agctacttag atacgattct ttcatcgaca gatacggtag ctacttctca
    25981 aattgcagct gattacggaa tgtcggcaat tgctctaaac aaattgctaa acgagttagg
    26041 tgttcagcat aaagttagcg gacaatggat actttaccga aaacatatga accaaggata
    26101 cacaaaatcg cacacaagtg agataccgaa agccgatggc ggcactaaag ttgtaatgaa
    26161 taccaaatgg acacagaaag ggcgagtgtt tatttacaac ttattaatcg cagagggcta
    26221 ttaccctcaa atggatttag aggaaattgg ttagaaagga gttttagtat gactgacatt
    26281 gcagaaatca ctcaacgaga tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc
    26341 ttaacttaca ccatgcttgc tgaaagattt ggaattagca aaagctactt atctttaatt
    26401 ttaaacggta aaaagacttc tgcagaagca aacagaatta tagattcgat tatcactatg
    26461 tacgaattgt aaagggagga acagctaatg caatatctag aagcaaaaat cccaattcca
    26521 gaaggctatg taattatctc ccaagtggat tatgaggagt taaaaaaagc tgatgatact
    26581 ggtagatgga tgacgttgcc agaagtgcta gaacggatta acagaaaata tgattggttt
    26641 acttctagag ttttaaagaa cccaagatat agaaatatta tcgatatcga gaaaaacaaa
    26701 aatggatttg tctattatcc agttgaagga agagacacat atctattttt aagaagtaaa
    26761 acacttgaat ttttagaaac aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa
    26821 atttaataga gcgctagtgt tcagcgcacc gctaatcatc tacgctttag gactttgggg
    26881 aagcaggcaa gcgttgatag gaacgattgt ttacatggtt tggattttta tggggcttga
    26941 tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat
    27001 agaaaagaga aactagaatg tatattacta ttactcagtt taattctttc actaatttct
    27061 ctattgggaa gtttgtattt ttgatatcaa caattttgtg gatttgggtt ctatttagag
    27121 taaatatcat ttttgtttgg tgattaagga gtttattaaa attaattttc cctacttcaa
    27181 atgcaaggta acaagatata gcacttttag gaggaatatt tagcggtaca gcttcagagt
    27241 atattctatt atcatcgact gatcctcctt tagcaattaa tttcttaaat ggagtagctg
    27301 accatctgcg gtttaagtta gcggatgaaa gttctaaatt aactaacata gctggttctg
    27361 ttgaatagtt agaaatgatt acttttgtat aaatcatatc tgcagcgaag taagaagcat
    27421 taagttcaac ttctagttga gggcgcattc cttttatttt tatccctgtt aaaaacgtac
    27481 tcaatataaa acttattatt gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa
    27541 taaacaccac cagtttttaa ctaaattata ccaaaaagga gagaagaaat aatgcaagaa
    27601 ttagtaattt tgaaaaataa agaagctgtg actacgagct tacaagtggc agaaagcttc
    27661 gagaaaaaac atcaacatgt tttaagagat attgatgcac taaaaaaaga tgtgtccaat
    27721 tttggacaga tgtttgtaga aggtaatgaa ccagattcat atggcagaaa tcgacgagtt
    27781 ttcttcatta gtagagatgg atttttcttg ctggctatgg ggtttacagg aaagaaagct
    27841 atctacttca aacaaaaata cattgaagca ttcaacgaaa tggaagatgt tattcgcaag
    27901 aatactgttc ctcaaacaat tgaagatatg atgatctatc aattagaaga aatgaaagat
    27961 gttaaaaaag atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt
    28021 gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa agaaagccga
    28081 gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc
    28141 ttttcaatcc caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt
    28201 attgaaatgt ggttgccaga aactgcgatc cgtatggaaa ttgatcaact gaacagacaa
    28261 cagagacttt ttggtgatga caatgaatag agctgaagcg ctaagaatag ggacggcaat
    28321 tgctaattgc tggtggaaat actataaacc aatcatccta agccaacaac atattgacaa
    28381 gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg caagcaaaga gtcacaaaga
    28441 aaacacatca taaggagatt ttagcatatg gaaaaagaac tttccactct agatcaatat
    28501 ttgattgatc ctgattgggg caagccgaaa attgaggaaa caagtggtcg aaaaatcaga
    28561 cgaaatcttt tgacgaatga agaactagct tgggatcaag atgatttagg caaccatgta
    28621 actatttggg atcatgttta tcttatccat ctatcgaagc attcgaataa acctgaatat
    28681 atttacgtca tcgaagatgg cttgattgat gcgctagaag agtacgacag agataacttg
    28741 attgatatct cttattacgg accaggtaag aaatacattg ctgaaatgga ggcagaattt
    28801 gatgagtgaa atcaaaggga caacgaactt tgaaaaactt tttagtcgta agttaaataa
    28861 aattctcaag aaaaaaggaa attttgatta tttatcttgg gctcacgcgt gggagattat
    28921 gaaaaagaat gatccacagg caacggtaac tattaatgag tacaaacact acagggttgt
    28981 ttctggaact catcaagact ttcttgttga ggaatataaa ccttttctta tggatgaaac
    29041 tgggacttat gtatctgtct cagtaacggt taaaggacac acggaaaccg aattatttcc
    29101 tgttttagat tatcgaaacc aaccagttgt taaaccaaat gcaatgcaaa tcaataactc
    29161 attgaagcga tgctttgtga aagcattggc tctacacgga ctgggattat atgtatttca
    29221 aggggaagat attccaacac cacctagaat cgatacaaag aaattaaaca tgctagagac
    29281 gattctagaa gctttcaatg agcagatggg taaagatatg accaaaacct taatcgaata
    29341 tgttaatgag cagacagata aattagggct cttagctgat aacgttgaaa ctattgaaca
    29401 gttaagctat gagcaatgtg ccttgatgga gcgagcaata gcagctaaga gaaaagaatt
    29461 agataagaag tgatatgagt gtttaaacca ttaatcgatt catattcagc ggttctgaaa
    29521 aagttcaaag gaaaagacat aagcgcaacc atcaatgagg aagtgaacat tgatcgacta
    29581 aagacgatgt atgacggcta cgatggtgat cgagtcattg aaattcgttt tattgatcct
    29641 agacgtttca ccgtacagca acgaaacttc atctatgcgc tgataggcga tatttttatc
    29701 gatacaggca tgccaacgga cttctggaag gaattcttct actttcgttt tgaaggtgtc
    29761 acagggcgca aaataagcct gaaagacgaa tcgaatacaa ctgtgagtga tgccaatgtc
    29821 ttagcaaata tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat
    29881 gagattttac ctgcgaatca agaatattac ttctacaaat gcatcacaaa aagagtctgc
    29941 tgcatctgtg gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga
    30001 aagcgcaaag aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac
    30061 acagagaagc acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc
    30121 aaattaaacc agaaaacaat caaaaagtta aatattggag gggaaaaatg acagaacatc
    30181 gaagttatta tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata
    30241 ctaagttgtt atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag
    30301 gcaatgagta ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa
    30361 gtgatttgat taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc
    30421 aaataatcaa taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc
    30481 ctattgacga aaaagtcaaa gataataata catctattaa taatacattt aataatacaa
    30541 aagaatatat aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata
    30601 aatacggaga gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag
    30661 aattccctaa tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg
    30721 gtaagactta taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg
    30781 aacctaagaa cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat
    30841 gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat
    30901 caaaagcacc attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga
    30961 aatagcgaat tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact
    31021 cgaagaagct ggaattgaag gaattaagaa tgatcgttcg agttggagta aactcagata
    31081 ctatttcgca ctctatatcg atggaacgca attaagaaat ttagcatata ccaaattact
    31141 attcattgat tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaaac
    31201 aagatggacc taaagacatt tacagcacag atcgaattaa tgcatcaaga agctttaaga
    31261 caaagtgtgt cgtacgaaga caagtggctc aacacatttc atggcggacg tgagagcgca
    31321 cttgatcaag tactcaaatt actgaaagga gaatgtcggg atggataaga aagcggcaat
    31381 gcagcgaatt atcgaattga cttattcaga agattggcag gaagataaaa aaatagttgc
    31441 agaagtccaa aagctcggta aatcaatgtg gactgaaaag cccaaacgga gaacgccgag
    31501 aaagattgca atctggcatg gtgatcgaat tctagtaacg ggtaccgcta aacagttatc
    31561 tgaaattact ggtctgagca aaaatattat ttgggataga gctaggagct tatggattga
    31621 ttcaaaagga agacagttta ggtatgtgga ggagaaataa tggacgaact aatcgcgaaa
    31681 gtagagcagt gggcaaaaga taagggattg gatcaagcgg atttcagcaa gcaaatgtta
    31741 aaaacggtcg aagaaatcgg ggaagttgcc gcttctctag ctagaaaaga tgaacatggt
    31801 ttaagagacg gaattggcga tgtagtagta accttgatca tcttggcaat gcaaaatgat
    31861 atggatttat acgagtgctt ggattttgct tatgacgaaa tcaaaggtcg gactggaaag
    31921 atggtcaatg gtgttttcgt taagtccagt gacctgaaat aattccatta atggaagaaa
    31981 taactgaata ggaggataga catgattgat gcaaaacaag tgcaaaaaca gaaagatgga
    32041 atgttgatgt ttgaagcata tgttctacca tttcttaatc aatttgaagt tttggaatgc
    32101 tctgccagcg gagaagaatt ggagtatgtt gtaattcgtg aaaccaaaga aaacgttcag
    32161 aagctaaacg aatttctttg cactattaac tgttgggata tgatagctcc aggattttta
    32221 tgtccagcta tgggagaatt tttggaatat tgtcgtcttg aagatgcagg cactttggat
    32281 ttagcttatc tggtatacaa ctacctaaac atcaacacag atcatttgtg gtttggcact
    32341 gccgaaagaa aatgggtagt ccgctaacca ccaatatcaa tgaaaaaagg cggtttcctg
    32401 aaagtggaat tgccgccatt gctgtaaggt tctttacgcg actcaaggtc acagaaatat
    32461 aaatgcaaat ctcttttgaa gtgatccaaa gctctcggaa aatggaagtt ttttgtttca
    32521 aagaataaat gagatttgcg tcaagaacct gagagttatt tccaaaaaag cttccaaaga
    32581 accgaatgag ctaaggacat catgtgtaaa cacataatat agctctcctt tcacggctaa
    32641 tcataacatg aaaaatattc gatgtcagta ggaaaaaagg tgaattatga aaagaatatt
    32701 aaattatccc ggcagtaaca gattctgcaa ttaaactccg aagctgatgt atcagtatca
    32761 actgtagaat attatgagcg tggagaaagt caagcagtca caaatgtaaa aatcgataac
    32821 gtcaacggtc ttatctgttt agatgaaggc gatagtatga atgaaattgc tgcagaccat
    32881 gaattatcag aaactgcttt aattgatcaa atcgaattag ctagacaata ctttgttcca
    32941 tatgcagata cctggtccaa gcataagaat gacatcattt ttccagagga acaatgagag
    33001 caactgatcc agtaattatc cttgaggaag ccaaatttat ttggactcac gaagagatag
    33061 agcaagcacg cttgctcttt tctcaaggag ttaagccgag caaagtagct gaaataatgg
    33121 gtcaaaagat tcttgatgtc ggattgcttt tgctccatct agcagaaaaa atttgatttg
    33181 aggtgaaaat gatgattcaa cttgcaggca tccaaacagg gaaaatttat ttttctggag
    33241 aaagcaaaag cgaagccaac caatggctat tgaaaactta cacgaacaat aagaagctaa
    33301 ggaaaaaata tcctgatttg ttgctaaagg atgatcagat tatgccggaa ccgatgattt
    33361 tgacccgtaa ggagggaagt tgaaactaaa aaaccagccg tgtgggctgg agttgctatt
    33421 tctttttaat aatgagtttt tctaaattta attcattctc acgagtttgc gtccaataat
    33481 cagaataata ttttaattgt ttaagagtag gaatgtcgtc ttcggtaaaa ttagaaaata
    33541 attctggaga attatcttt
//
LOCUS       DAZUMI010000018        33127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000018 DAZUMI010000000
VERSION     DAZUMI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.018"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACG81C_000719"
     CDS             complement(27..1259)
                     /locus_tag="ACG81C_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5063905.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACG81C_000720"
     CDS             1514..2083
                     /locus_tag="ACG81C_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5063906.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACG81C_000721"
     CDS             complement(2261..2701)
                     /locus_tag="ACG81C_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5063907.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACG81C_000722"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACG81C_000722"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5063908.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACG81C_000723"
     CDS             3655..4578
                     /locus_tag="ACG81C_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5063909.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACG81C_000724"
     CDS             complement(4803..5042)
                     /locus_tag="ACG81C_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063910.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACG81C_000725"
     CDS             complement(5047..5466)
                     /locus_tag="ACG81C_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5063911.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG81C_000726"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG81C_000726"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS5063912.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACG81C_000727"
     CDS             complement(7038..7673)
                     /locus_tag="ACG81C_000727"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5063913.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACG81C_000728"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACG81C_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACG81C_000729"
     CDS             complement(8015..8650)
                     /locus_tag="ACG81C_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5063914.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACG81C_000730"
     CDS             9162..10304
                     /locus_tag="ACG81C_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5063915.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACG81C_000731"
     CDS             10297..10503
                     /locus_tag="ACG81C_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS5063916.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACG81C_000732"
     CDS             10582..11775
                     /locus_tag="ACG81C_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5063917.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACG81C_000733"
     CDS             11980..13119
                     /locus_tag="ACG81C_000733"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5063918.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACG81C_000734"
     CDS             13112..13912
                     /locus_tag="ACG81C_000734"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5063919.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACG81C_000735"
     CDS             13912..14739
                     /locus_tag="ACG81C_000735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5063920.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACG81C_000736"
     CDS             14744..15451
                     /locus_tag="ACG81C_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5063921.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG81C_000737"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG81C_000737"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5063922.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG81C_000738"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG81C_000738"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5063923.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG81C_000739"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG81C_000739"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5063924.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG81C_000740"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG81C_000740"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5063925.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACG81C_000741"
     CDS             19036..21069
                     /locus_tag="ACG81C_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063926.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACG81C_000742"
     CDS             21113..22393
                     /locus_tag="ACG81C_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5063927.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACG81C_000743"
     CDS             22603..23409
                     /locus_tag="ACG81C_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063928.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACG81C_000744"
     CDS             23421..24641
                     /locus_tag="ACG81C_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063929.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACG81C_000745"
     CDS             24631..26217
                     /locus_tag="ACG81C_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5063930.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACG81C_000746"
     CDS             26256..28394
                     /locus_tag="ACG81C_000746"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5063931.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="ACG81C_000747"
     CDS             complement(28762..29745)
                     /locus_tag="ACG81C_000747"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5063932.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="ACG81C_000748"
     CDS             30123..31514
                     /locus_tag="ACG81C_000748"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5063933.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="ACG81C_000749"
     CDS             31530..32570
                     /locus_tag="ACG81C_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5063934.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..>33127
                     /locus_tag="ACG81C_000750"
     CDS             32589..>33127
                     /locus_tag="ACG81C_000750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5063935.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNS"
BASE COUNT        10672 a         5739 c         6542 g        10174 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcaga
//
LOCUS       DAZUMI010000019        30742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000019 DAZUMI010000000
VERSION     DAZUMI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.019"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..964)
                     /locus_tag="ACG81C_000751"
     CDS             complement(20..964)
                     /locus_tag="ACG81C_000751"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5063936.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1260..2204)
                     /locus_tag="ACG81C_000752"
     CDS             complement(1260..2204)
                     /locus_tag="ACG81C_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5063937.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2425..3639
                     /locus_tag="ACG81C_000753"
     CDS             2425..3639
                     /locus_tag="ACG81C_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5063938.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3661..5676
                     /locus_tag="ACG81C_000754"
     CDS             3661..5676
                     /locus_tag="ACG81C_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS5063939.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5759..7132)
                     /locus_tag="ACG81C_000755"
     CDS             complement(5759..7132)
                     /locus_tag="ACG81C_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5063940.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7158..7442)
                     /locus_tag="ACG81C_000756"
     CDS             complement(7158..7442)
                     /locus_tag="ACG81C_000756"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5063941.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7444..9459)
                     /locus_tag="ACG81C_000757"
     CDS             complement(7444..9459)
                     /locus_tag="ACG81C_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5063942.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9638..9943)
                     /locus_tag="ACG81C_000758"
     CDS             complement(9638..9943)
                     /locus_tag="ACG81C_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5063943.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10032..11063)
                     /locus_tag="ACG81C_000759"
     CDS             complement(10032..11063)
                     /locus_tag="ACG81C_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5063944.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11270..12145
                     /locus_tag="ACG81C_000760"
     CDS             11270..12145
                     /locus_tag="ACG81C_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5063945.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12297..13139)
                     /locus_tag="ACG81C_000761"
     CDS             complement(12297..13139)
                     /locus_tag="ACG81C_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5063946.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13231..15108)
                     /locus_tag="ACG81C_000762"
     CDS             complement(13231..15108)
                     /locus_tag="ACG81C_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5063947.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15105..16526)
                     /locus_tag="ACG81C_000763"
     CDS             complement(15105..16526)
                     /locus_tag="ACG81C_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5063948.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16529..19918)
                     /locus_tag="ACG81C_000764"
     CDS             complement(16529..19918)
                     /locus_tag="ACG81C_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5063949.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20175..21566
                     /locus_tag="ACG81C_000765"
     CDS             20175..21566
                     /locus_tag="ACG81C_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063950.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21639..22286)
                     /locus_tag="ACG81C_000766"
     CDS             complement(21639..22286)
                     /locus_tag="ACG81C_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5063951.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22294..22980)
                     /locus_tag="ACG81C_000767"
     CDS             complement(22294..22980)
                     /locus_tag="ACG81C_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5063952.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22986..24059)
                     /locus_tag="ACG81C_000768"
     CDS             complement(22986..24059)
                     /locus_tag="ACG81C_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5063953.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24281..24955
                     /locus_tag="ACG81C_000769"
     CDS             24281..24955
                     /locus_tag="ACG81C_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5063954.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24960..26300
                     /locus_tag="ACG81C_000770"
     CDS             24960..26300
                     /locus_tag="ACG81C_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5063955.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26449..27435)
                     /locus_tag="ACG81C_000771"
     CDS             complement(26449..27435)
                     /locus_tag="ACG81C_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5063956.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28159..29190
                     /locus_tag="ACG81C_000772"
     CDS             28159..29190
                     /locus_tag="ACG81C_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5063957.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29299..29724
                     /locus_tag="ACG81C_000773"
                     /pseudo
     CDS             29299..29724
                     /locus_tag="ACG81C_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            29837..>30742
                     /locus_tag="ACG81C_000774"
     CDS             29837..>30742
                     /locus_tag="ACG81C_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS5063958.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCYVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         9163 a         5759 c         5531 g        10289 t
ORIGIN      
        1 agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa gcacttgttg
       61 atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact tcacgaacac
      121 cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg tattcaccag
      181 ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct tttacgatac
      241 gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg atctcataag
      301 cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata cgatggtctt
      361 tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat gcagcaactt
      421 ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca acttttaatt
      481 tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta ccgataacac
      541 gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct actgggtttg
      601 aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg atttggcgca
      661 tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt tggttgatac
      721 cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct gaccatacag
      781 ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc ccttctgctt
      841 tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg atcattgcat
      901 aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga cttgtttttt
      961 tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag tatagtcttg
     1021 tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt tgatggaaac
     1081 gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat cccctgataa
     1141 tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata
     1201 agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag attttcttct
     1261 taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa taatttgaat
     1321 aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga aaaactcgtt
     1381 tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa
     1441 gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc aaaatgagct
     1501 gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa tactctaagc
     1561 atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa taaatgagtc
     1621 aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg actgtatgta
     1681 tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt ttttccgccg
     1741 cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat caaaaaagaa
     1801 gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa ggtttcttct
     1861 tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct ttgagtagct
     1921 tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc attaataatt
     1981 tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt atgctcggga
     2041 acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa tagatcccct
     2101 cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt gatattcatt
     2161 ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc
     2221 agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta ttagttcacg
     2281 ctgtactgct attatatgca aagctatttt tggtttcata aagactttct atggttggga
     2341 catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat atttttgata
     2401 agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa attttctctg
     2461 ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat tcccagtatg
     2521 tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac tgctccctct
     2581 tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat cggacaaaaa
     2641 caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc cctcttctca
     2701 caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt tggattgatc
     2761 aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg agcctctatg
     2821 attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt tgttgcagga
     2881 cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag cttacctatt
     2941 cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcgcatggc
     3001 acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta tggtattttt
     3061 ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg
     3121 acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca
     3181 agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt
     3241 acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg
     3301 tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac
     3361 ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc
     3421 ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct
     3481 tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg
     3541 attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga
     3601 gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa
     3661 atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc
     3721 catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg
     3781 tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct
     3841 tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat
     3901 ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga
     3961 catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc
     4021 tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat
     4081 atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa
     4141 acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa
     4201 ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga gtccgatgta
     4261 gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa
     4321 gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa
     4381 cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct
     4441 tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg tagtgatttg
     4501 ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca
     4561 ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca
     4621 ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg
     4681 tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc
     4741 gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg
     4801 tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa
     4861 ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc
     4921 ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa
     4981 gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc
     5041 gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc
     5101 gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta
     5161 ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga acaagatgct
     5221 ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg
     5281 atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat
     5341 gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca
     5401 agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat
     5461 gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct
     5521 atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat
     5581 agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca
     5641 cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat
     5701 cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt
     5761 aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata
     5821 cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta
     5881 gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga
     5941 ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat
     6001 tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag
     6061 aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc
     6121 ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata
     6181 cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta
     6241 cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc
     6301 cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta
     6361 cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa
     6421 gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc
     6481 tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc
     6541 cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt
     6601 ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc
     6661 cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta
     6721 aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt
     6781 tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc atgatcaaag
     6841 cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta
     6901 ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga
     6961 ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga
     7021 tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga
     7081 ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt
     7141 cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc ttttacttct
     7201 tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta
     7261 tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca
     7321 tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg
     7381 atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc
     7441 atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa
     7501 taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca aagcccgaag
     7561 atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat gggctggatt
     7621 atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt cgtttacgcc
     7681 atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa acgcaccata
     7741 ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat tttctgataa
     7801 tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg
     7861 gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa
     7921 aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt
     7981 taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc
     8041 tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat
     8101 gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga
     8161 aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc
     8221 tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct
     8281 aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc
     8341 ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct
     8401 cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc
     8461 atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg
     8521 aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa acgaagcaaa
     8581 ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa
     8641 atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa
     8701 tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat
     8761 gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt
     8821 tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc
     8881 gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata
     8941 atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat
     9001 caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct
     9061 aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat
     9121 ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa
     9181 aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg
     9241 ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc
     9301 tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag gagttaattg
     9361 tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc
     9421 tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt
     9481 atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat
     9541 tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa agcaggtgac
     9601 caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca tgtttatttt
     9661 ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt
     9721 caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag
     9781 ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt
     9841 ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg
     9901 aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta
     9961 gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc
    10021 ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa
    10081 attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat
    10141 gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct
    10201 tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg
    10261 ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct
    10321 tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg
    10381 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    10441 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    10501 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    10561 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    10621 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    10681 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    10741 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    10801 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    10861 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    10921 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    10981 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    11041 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    11101 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    11161 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    11221 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    11281 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    11341 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    11401 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    11461 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    11521 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    11581 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    11641 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    11701 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    11761 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    11821 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    11881 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    11941 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    12001 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    12061 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    12121 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    12181 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    12241 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    12301 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    12361 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    12421 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    12481 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    12541 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    12601 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    12661 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    12721 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    12781 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    12841 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    12901 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    12961 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    13021 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    13081 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    13141 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    13201 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    13261 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    13321 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    13381 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    13441 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    13501 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    13561 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    13621 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    13681 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    13741 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    13801 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    13861 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    13921 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    13981 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    14041 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    14101 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    14161 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    14221 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    14281 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    14341 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    14401 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    14461 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    14521 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    14581 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    14641 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    14701 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    14761 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    14821 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    14881 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    14941 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    15001 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    15061 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    15121 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    15181 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    15241 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    15301 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    15361 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    15421 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    15481 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    15541 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    15601 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    15661 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    15721 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    15781 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    15841 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    15901 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    15961 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    16021 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    16081 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    16141 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    16201 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    16261 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    16321 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    16381 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    16441 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16501 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16561 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16621 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16681 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16741 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    16801 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    16861 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    16921 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    16981 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17041 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17101 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17161 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17221 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17281 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17341 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17401 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17461 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17521 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17581 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17641 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17701 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    17761 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    17821 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    17881 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    17941 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18001 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18061 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18121 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18181 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18241 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18301 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18361 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18421 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18481 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18541 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18601 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    18661 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18721 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    18781 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    18841 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    18901 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    18961 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19021 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19081 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19141 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19201 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19261 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19321 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19381 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19441 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19501 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19561 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19621 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19681 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19741 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    19801 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    19861 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    19921 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    19981 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20041 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20101 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    20161 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20221 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    20281 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    20341 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    20401 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    20461 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    20521 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    20581 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    20641 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    20701 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    20761 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    20821 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    20881 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    20941 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    21001 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    21061 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    21121 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    21181 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    21241 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    21301 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    21361 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    21421 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    21481 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    21541 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    21601 ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc
    21661 caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg
    21721 agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct
    21781 tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt
    21841 ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac
    21901 aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat
    21961 ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga
    22021 tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa
    22081 tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact
    22141 tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt
    22201 gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt
    22261 atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt
    22321 caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg
    22381 agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac
    22441 ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg
    22501 ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt
    22561 aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt
    22621 tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa
    22681 aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc
    22741 tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt
    22801 cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc
    22861 aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac
    22921 ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat
    22981 ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac
    23041 aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa
    23101 aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga
    23161 gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg
    23221 aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc
    23281 atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc
    23341 gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata
    23401 gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt
    23461 acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa
    23521 gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga
    23581 aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt
    23641 ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt tttaaattca
    23701 tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca
    23761 ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct
    23821 ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca
    23881 tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct
    23941 gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata
    24001 ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt
    24061 tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac
    24121 tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca
    24181 tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt
    24241 ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt
    24301 agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat
    24361 aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct
    24421 tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg
    24481 gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa
    24541 gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga
    24601 actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt
    24661 tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca
    24721 agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg
    24781 aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac
    24841 gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt
    24901 ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa
    24961 tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat
    25021 ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat
    25081 acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag
    25141 agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca
    25201 gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga
    25261 tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc
    25321 aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa
    25381 atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga
    25441 atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat
    25501 tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg
    25561 gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac
    25621 aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa
    25681 atagtttgga acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca
    25741 tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa
    25801 tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat
    25861 atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta
    25921 ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt
    25981 tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg
    26041 attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca
    26101 gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga
    26161 aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc
    26221 atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc
    26281 cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca
    26341 cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg
    26401 aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt
    26461 attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac
    26521 atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg
    26581 aataactgta tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc
    26641 atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat
    26701 gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt
    26761 gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt
    26821 tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt
    26881 acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa
    26941 tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt
    27001 gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat
    27061 tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc
    27121 agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc
    27181 ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc
    27241 atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct
    27301 agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg
    27361 caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa
    27421 atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt atattgttac
    27481 ttataaattt attctatagg agacgattgt tttctacaaa tatttagatt ggaatttgat
    27541 taaaacaatt taattgaaat ttgaaaacat ttatgttaat gaaagtcata ttctaataaa
    27601 atgtcatttg tttctatatg aatttttatg aaaaatttca tataatttcc actcgttgtg
    27661 atggtttaat ttatccaatc catcaataag caaacaactt atataaaaaa ggattaattc
    27721 tcaatataat tgctcctacc atagaataaa tcttcatgta aagtaatgaa gtaccaacta
    27781 tttctaatac ttctttaaga attatcccaa caatatcgtt agacaaacca ttattgaata
    27841 aatggagagc caatcgattc tgaaaaaatg ttttctaatt tttcggaaat aaaagaaaga
    27901 gcctcctgcc attagaacga accaaatcca tttcattgct catgaagtaa ccacatagct
    27961 aaaaagagga taaatcaatt aaaagatcaa aagaatgact gcactaataa aataactaaa
    28021 aatgtatctt cttacttcat atcttttagc tagtacttag ttaaaaattt gcttcctaag
    28081 atcaagacaa actgtctatc ttcatttgat gaaatgcggt aaaatataga aaatgaacac
    28141 tattggaggg aatttgtaat ggacagcaaa actagaaaac aactgcaaga aaagttaaag
    28201 caacgtacac acttgctccc ttccgtcttt caacagtttt tagaagaaaa tcgttcttcc
    28261 ctttcattag gcagtcgcta tgaatacgca aaagattttc atctgtttac tgattatttg
    28321 agaaaagtat tttctgaaga agtggatgat cagctgatta tccatttaga aaaacaagat
    28381 tatcagaatt tcttagctaa aatctatcga catactcgtt cgttccaaac tacagcgaac
    28441 caagaaacgg agcaattgtt tgaaaatagc tattacggga tttcaaggaa gcaatattca
    28501 ttaaatcatt ttttacgatt tttgtatcaa aaaggactga ttgaagaaga gatttctctt
    28561 ttgggtacaa gtttaccgga aacaaccaaa gcagctccgc gatatatcga tgcagaactt
    28621 ttcaaagatt ttgagcattt gtttatcccg attgaaggct ttcaaacaag cagagaagct
    28681 agctttgaag aaaagaatag accgaggaac cttgcaatta ctgccattct tctgtattgc
    28741 ggattaaaga tccatgaagt agtagaactt aaacgtaaag atgtatcttt gaccaagcag
    28801 attcttaatc tgaatagaaa agaaaacaga ctgaataaag taccaatacc aaaagaagcg
    28861 atgccatttc tggagaacta ttttaagtta acagcacaaa atacggacga aaattctttt
    28921 gtttttcgtt cctcacatga tcagcaaatt tctcctcgga ctattcgcca aattttgagc
    28981 aaaattacag tatataaaaa cgtatcgcca gaactatgca gaaaaacata tcgttattat
    29041 gcagaactat atttagatga tgctgacgcg gtaaattatt tatgtggtaa tcgatacctc
    29101 tcatcccatt cttttcaaga tatctgggaa aagatggttg atttttctta tcacgaatta
    29161 gcggcattcg ttcgggtttc ttccatataa aacaaatgta tgatcttttt ttcacaataa
    29221 actatgttat tatataaagg attatgttac gattatcttg aatttgtata ttacttaaca
    29281 aaagataatg gagtgtgaat gatggagacc ataacgtatt cccctttgaa cttatttacg
    29341 aattttcaag aagctgcagc taacttttcc gagaccccga ttatctttga tgagccactt
    29401 tcagctttcc cagaattagg acttgaaaca acttacaaaa aatgctgcga aagtgtgttg
    29461 aaacgggctt atcaattggc tcatcttgga gtgaaatccg gagacaaagt tattatttat
    29521 aagagttccg cttttgacac ataccttcta gctgtttctg tgtcttacct tggtgcagtg
    29581 cctgtcatga cttcttatca tctaccaaca acaacaatgg aagtatttat cgatcgttta
    29641 gaagaccctt tcattttatt tgatgatgag acaaaaaagc gagtgagaga aatttcaaat
    29701 ggaacaaaat caaaacagat tcctaacccg tggtgctagt taattttttc ctagaggaaa
    29761 atgtctaaac atgttggtaa atcaacaaaa aaacgcatat agtagtctca aaactcccaa
    29821 gaaatgagga ctacatatgc gttcatcaaa atatactgaa cattatacag atacttttat
    29881 aacattcaag gaaattatga gaactgtttc taacatatac cacaattgtg taccagataa
    29941 gattaaaaac cgaagaaata ctgatcaact gaagcaacgc gatacagtga ttattgcttg
    30001 tgttatatgg ggcataatta atggctatac tagccaaaga gccacgtata gagcggtttg
    30061 ttacgtctta tttcctaatg gtgactttcc tagtaggagt cgattcactc gcttaagttc
    30121 aaacttagct tacactatca agattattcg atactttttc atcaagaagc tcaccaaagg
    30181 tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag ttagaaatag
    30241 acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa aaaaatctta
    30301 tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga ttacttactc
    30361 aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg aagaagcgaa
    30421 tcttcccaat atcctagggg acaaaggata tattagccat aaaatccatg aaaagcttgc
    30481 ccttaagggc attactatat ccgttccgcc acgtaaaaac atggataaat cagaaaaact
    30541 agaccacagc ttacttggaa aacaaagaaa aacagttgag actgtttttt cttccttaga
    30601 aaaattaggt tgtcaaaatt tcaactcacg ttctgttaaa gggctagaga gtagatttga
    30661 aagcatatta ctggcttaca gtgttctatt aagtcgagca caacgacgtt ttgaaggaac
    30721 tttgagatat tctttaggat at
//
LOCUS       DAZUMI010000020        29523 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000020 DAZUMI010000000
VERSION     DAZUMI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.020"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..726)
                     /locus_tag="ACG81C_000775"
     CDS             complement(61..726)
                     /locus_tag="ACG81C_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5063959.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACG81C_000776"
     CDS             complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACG81C_000776"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5063960.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACG81C_000777"
     CDS             complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACG81C_000777"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5063961.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACG81C_000778"
     CDS             complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACG81C_000778"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5063962.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3040..3360
                     /locus_tag="ACG81C_000779"
     CDS             3040..3360
                     /locus_tag="ACG81C_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5063963.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3472..4329)
                     /locus_tag="ACG81C_000780"
     CDS             complement(3472..4329)
                     /locus_tag="ACG81C_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5063964.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4437..5741)
                     /locus_tag="ACG81C_000781"
     CDS             complement(4437..5741)
                     /locus_tag="ACG81C_000781"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5063965.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5738..6247)
                     /locus_tag="ACG81C_000782"
     CDS             complement(5738..6247)
                     /locus_tag="ACG81C_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063966.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6422..8557)
                     /locus_tag="ACG81C_000783"
     CDS             complement(6422..8557)
                     /locus_tag="ACG81C_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5063967.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8640..10295)
                     /locus_tag="ACG81C_000784"
     CDS             complement(8640..10295)
                     /locus_tag="ACG81C_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5063968.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10297..10611)
                     /locus_tag="ACG81C_000785"
     CDS             complement(10297..10611)
                     /locus_tag="ACG81C_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5063969.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10781..11041
                     /locus_tag="ACG81C_000786"
     CDS             10781..11041
                     /locus_tag="ACG81C_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5063970.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11055..11369
                     /locus_tag="ACG81C_000787"
     CDS             11055..11369
                     /locus_tag="ACG81C_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5063971.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12099..12653)
                     /locus_tag="ACG81C_000788"
     CDS             complement(12099..12653)
                     /locus_tag="ACG81C_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5063972.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12779..14708)
                     /locus_tag="ACG81C_000789"
                     /pseudo
     CDS             complement(12779..14708)
                     /locus_tag="ACG81C_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15014..15730)
                     /locus_tag="ACG81C_000790"
     CDS             complement(15014..15730)
                     /locus_tag="ACG81C_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5063973.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACG81C_000791"
     CDS             complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACG81C_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5063974.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACG81C_000792"
     CDS             complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACG81C_000792"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5063975.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19200..20660)
                     /locus_tag="ACG81C_000793"
     CDS             complement(19200..20660)
                     /locus_tag="ACG81C_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5063976.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20650..21945)
                     /locus_tag="ACG81C_000794"
     CDS             complement(20650..21945)
                     /locus_tag="ACG81C_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5063977.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22277..22954)
                     /locus_tag="ACG81C_000795"
     CDS             complement(22277..22954)
                     /locus_tag="ACG81C_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5063978.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22984..24075)
                     /locus_tag="ACG81C_000796"
     CDS             complement(22984..24075)
                     /locus_tag="ACG81C_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5063979.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24176..24610)
                     /locus_tag="ACG81C_000797"
     CDS             complement(24176..24610)
                     /locus_tag="ACG81C_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5063980.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25194..26651)
                     /locus_tag="ACG81C_000798"
     CDS             complement(25194..26651)
                     /locus_tag="ACG81C_000798"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5063981.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26648..28543)
                     /locus_tag="ACG81C_000799"
     CDS             complement(26648..28543)
                     /locus_tag="ACG81C_000799"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5063982.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28601..>29523)
                     /locus_tag="ACG81C_000800"
     CDS             complement(28601..>29523)
                     /locus_tag="ACG81C_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5063983.1"
                     /translation="MINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIK
                     QMKTRMDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEA
                     LNPFRKQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYD
                     AILTTEQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEE
                     NQIKIHKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQ
                     RKIKRVFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT         8213 a         6394 c         5048 g         9868 t
ORIGIN      
        1 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
       61 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
      121 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
      181 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
      241 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
      301 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
      361 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
      421 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
      481 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
      541 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
      601 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
      661 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
      721 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
      781 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
      841 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
      901 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
      961 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     1021 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     1081 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     1141 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     1201 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     1261 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     1321 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     1381 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     1441 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     1501 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     1561 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     1621 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     1681 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     1741 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     1801 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     1861 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     1921 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     1981 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     2041 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     2101 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     2161 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     2221 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     2281 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     2341 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     2401 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     2461 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     2521 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     2581 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     2641 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     2701 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     2761 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     2821 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     2881 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     2941 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     3001 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     3061 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     3121 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     3181 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     3241 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     3301 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     3361 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     3421 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     3481 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     3541 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     3601 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     3661 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     3721 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     3781 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     3841 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     3901 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     3961 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     4021 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     4081 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     4141 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     4201 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     4261 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     4321 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     4381 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     4441 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     4501 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     4561 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     4621 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     4681 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     4741 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     4801 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     4861 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     4921 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     4981 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     5041 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     5101 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     5161 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     5221 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     5281 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     5341 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     5401 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     5461 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     5521 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     5581 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     5641 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     5701 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     5761 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     5821 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     5881 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     5941 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     6001 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     6061 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     6121 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     6181 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     6241 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     6301 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     6361 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     6421 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     6481 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
     6541 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
     6601 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
     6661 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
     6721 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
     6781 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
     6841 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
     6901 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
     6961 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
     7021 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
     7081 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
     7141 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
     7201 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
     7261 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
     7321 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
     7381 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
     7441 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
     7501 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
     7561 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
     7621 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
     7681 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
     7741 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
     7801 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
     7861 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
     7921 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
     7981 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
     8041 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
     8101 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
     8161 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
     8221 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
     8281 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
     8341 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
     8401 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
     8461 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
     8521 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
     8581 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
     8641 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
     8701 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
     8761 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
     8821 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
     8881 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
     8941 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
     9001 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
     9061 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
     9121 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
     9181 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
     9241 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
     9301 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
     9361 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
     9421 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
     9481 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
     9541 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
     9601 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
     9661 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
     9721 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
     9781 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
     9841 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
     9901 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
     9961 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    10021 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    10081 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    10141 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    10201 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    10261 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    10321 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    10381 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    10441 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    10501 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    10561 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    10621 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    10681 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    10741 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    10801 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    10861 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    10921 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    10981 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    11041 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    11101 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    11161 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    11221 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    11281 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    11341 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    11401 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    11461 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    11521 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    11581 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    11641 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    11701 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    11761 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    11821 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    11881 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    11941 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    12001 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    12061 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    12121 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    12181 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    12241 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    12301 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    12361 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    12421 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    12481 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    12541 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    12601 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    12661 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    12721 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    12781 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    12841 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    12901 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    12961 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    13021 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    13081 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    13141 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    13201 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    13261 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    13321 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    13381 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    13441 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    13501 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    13561 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    13621 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    13681 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    13741 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    13801 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    13861 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    13921 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    13981 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    14041 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    14101 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    14161 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    14221 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    14281 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    14341 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    14401 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    14461 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    14521 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    14581 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    14641 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    14701 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    14761 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    14821 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    14881 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    14941 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    15001 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    15061 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    15121 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    15181 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    15241 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    15301 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    15361 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    15421 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    15481 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    15541 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    15601 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    15661 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    15721 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    15781 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    15841 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    15901 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    15961 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    16021 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    16081 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    16141 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    16201 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    16261 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    16321 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    16381 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    16441 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    16501 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    16561 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    16621 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    16681 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    16741 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    16801 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    16861 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    16921 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    16981 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    17041 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    17101 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    17161 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    17221 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    17281 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    17341 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    17401 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    17461 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    17521 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    17581 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    17641 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    17701 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    17761 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    17821 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    17881 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    17941 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    18001 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    18061 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    18121 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    18181 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    18241 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    18301 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    18361 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    18421 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    18481 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    18541 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    18601 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    18661 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    18721 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    18781 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    18841 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    18901 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    18961 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    19021 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    19081 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    19141 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    19201 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    19261 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    19321 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    19381 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    19441 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    19501 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    19561 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    19621 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    19681 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    19741 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    19801 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    19861 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    19921 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    19981 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    20041 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    20101 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    20161 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    20221 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    20281 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    20341 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    20401 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    20461 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    20521 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    20581 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    20641 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    20701 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    20761 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    20821 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    20881 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    20941 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    21001 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    21061 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    21121 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    21181 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    21241 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    21301 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    21361 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    21421 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    21481 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    21541 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    21601 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    21661 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    21721 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    21781 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    21841 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    21901 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    21961 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    22021 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    22081 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    22141 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    22201 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    22261 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    22321 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    22381 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    22441 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    22501 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    22561 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    22621 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    22681 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    22741 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    22801 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    22861 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    22921 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    22981 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    23041 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    23101 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    23161 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    23221 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    23281 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    23341 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    23401 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    23461 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    23521 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    23581 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    23641 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    23701 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    23761 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    23821 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    23881 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    23941 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    24001 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    24061 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    24121 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    24181 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    24241 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    24301 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    24361 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    24421 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    24481 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    24541 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    24601 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    24661 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    24721 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    24781 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    24841 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    24901 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    24961 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    25021 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    25081 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    25141 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    25201 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    25261 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    25321 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    25381 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    25441 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    25501 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    25561 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    25621 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    25681 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    25741 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    25801 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    25861 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    25921 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    25981 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    26041 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    26101 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    26161 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    26221 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    26281 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    26341 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    26401 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    26461 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    26521 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    26581 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    26641 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    26701 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    26761 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    26821 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    26881 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    26941 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    27001 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    27061 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    27121 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    27181 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    27241 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    27301 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    27361 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    27421 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    27481 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    27541 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    27601 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    27661 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    27721 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    27781 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    27841 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    27901 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    27961 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    28021 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    28081 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    28141 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    28201 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    28261 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    28321 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    28381 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    28441 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    28501 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    28561 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    28621 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    28681 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    28741 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    28801 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    28861 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    28921 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    28981 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    29041 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    29101 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    29161 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    29221 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    29281 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    29341 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    29401 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    29461 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    29521 tca
//
LOCUS       DAZUMI010000021        28310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000021 DAZUMI010000000
VERSION     DAZUMI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.021"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..189)
                     /locus_tag="ACG81C_000801"
     CDS             complement(<1..189)
                     /locus_tag="ACG81C_000801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063984.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLF"
     gene            234..941
                     /locus_tag="ACG81C_000802"
     CDS             234..941
                     /locus_tag="ACG81C_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS5063985.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            934..1062
                     /locus_tag="ACG81C_000803"
     CDS             934..1062
                     /locus_tag="ACG81C_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063986.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            1120..1461
                     /locus_tag="ACG81C_000804"
     CDS             1120..1461
                     /locus_tag="ACG81C_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063987.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1454..2200
                     /locus_tag="ACG81C_000805"
     CDS             1454..2200
                     /locus_tag="ACG81C_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5063988.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            2206..2892
                     /locus_tag="ACG81C_000806"
     CDS             2206..2892
                     /locus_tag="ACG81C_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5063989.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2899..3732
                     /locus_tag="ACG81C_000807"
     CDS             2899..3732
                     /locus_tag="ACG81C_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS5063990.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            3749..4600
                     /locus_tag="ACG81C_000808"
     CDS             3749..4600
                     /locus_tag="ACG81C_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5063991.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            4597..4956
                     /locus_tag="ACG81C_000809"
     CDS             4597..4956
                     /locus_tag="ACG81C_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS5063992.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            4971..5132
                     /locus_tag="ACG81C_000810"
     CDS             4971..5132
                     /locus_tag="ACG81C_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5063993.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5129..5434
                     /locus_tag="ACG81C_000811"
     CDS             5129..5434
                     /locus_tag="ACG81C_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063994.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            5434..5751
                     /locus_tag="ACG81C_000812"
     CDS             5434..5751
                     /locus_tag="ACG81C_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5063995.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            5748..6134
                     /locus_tag="ACG81C_000813"
     CDS             5748..6134
                     /locus_tag="ACG81C_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5063996.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            6131..6466
                     /locus_tag="ACG81C_000814"
     CDS             6131..6466
                     /locus_tag="ACG81C_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063997.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            6466..6600
                     /locus_tag="ACG81C_000815"
     CDS             6466..6600
                     /locus_tag="ACG81C_000815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5063998.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            6612..7181
                     /locus_tag="ACG81C_000816"
     CDS             6612..7181
                     /locus_tag="ACG81C_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5063999.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            7178..7351
                     /locus_tag="ACG81C_000817"
     CDS             7178..7351
                     /locus_tag="ACG81C_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064000.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            7348..7653
                     /locus_tag="ACG81C_000818"
     CDS             7348..7653
                     /locus_tag="ACG81C_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064001.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            7677..7874
                     /locus_tag="ACG81C_000819"
     CDS             7677..7874
                     /locus_tag="ACG81C_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS5064002.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            7871..8077
                     /locus_tag="ACG81C_000820"
     CDS             7871..8077
                     /locus_tag="ACG81C_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064003.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            8077..8373
                     /locus_tag="ACG81C_000821"
     CDS             8077..8373
                     /locus_tag="ACG81C_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064004.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            8449..8862
                     /locus_tag="ACG81C_000822"
     CDS             8449..8862
                     /locus_tag="ACG81C_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5064005.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9546..9713
                     /locus_tag="ACG81C_000823"
     CDS             9546..9713
                     /locus_tag="ACG81C_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064006.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            9739..10083
                     /locus_tag="ACG81C_000824"
     CDS             9739..10083
                     /locus_tag="ACG81C_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5064007.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            10088..10369
                     /locus_tag="ACG81C_000825"
     CDS             10088..10369
                     /locus_tag="ACG81C_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064008.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10472..10786
                     /locus_tag="ACG81C_000826"
     CDS             10472..10786
                     /locus_tag="ACG81C_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5064009.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10764..12458
                     /locus_tag="ACG81C_000827"
     CDS             10764..12458
                     /locus_tag="ACG81C_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5064010.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12478..13656
                     /locus_tag="ACG81C_000828"
     CDS             12478..13656
                     /locus_tag="ACG81C_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5064011.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13619..14305
                     /locus_tag="ACG81C_000829"
     CDS             13619..14305
                     /locus_tag="ACG81C_000829"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5064012.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14305..15465
                     /locus_tag="ACG81C_000830"
     CDS             14305..15465
                     /locus_tag="ACG81C_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5064013.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15475..16350
                     /locus_tag="ACG81C_000831"
     CDS             15475..16350
                     /locus_tag="ACG81C_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064014.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16347..16658
                     /locus_tag="ACG81C_000832"
     CDS             16347..16658
                     /locus_tag="ACG81C_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064015.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16648..17001
                     /locus_tag="ACG81C_000833"
     CDS             16648..17001
                     /locus_tag="ACG81C_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5064016.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16991..17392
                     /locus_tag="ACG81C_000834"
     CDS             16991..17392
                     /locus_tag="ACG81C_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064017.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17385..17789
                     /locus_tag="ACG81C_000835"
     CDS             17385..17789
                     /locus_tag="ACG81C_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064018.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17801..18406
                     /locus_tag="ACG81C_000836"
     CDS             17801..18406
                     /locus_tag="ACG81C_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5064019.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18426..18788
                     /locus_tag="ACG81C_000837"
                     /pseudo
     CDS             18426..18788
                     /locus_tag="ACG81C_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18791..18973
                     /locus_tag="ACG81C_000838"
     CDS             18791..18973
                     /locus_tag="ACG81C_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064020.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18991..22410
                     /locus_tag="ACG81C_000839"
     CDS             18991..22410
                     /locus_tag="ACG81C_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5064021.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            22461..23198
                     /locus_tag="ACG81C_000840"
     CDS             22461..23198
                     /locus_tag="ACG81C_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5064022.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            23208..25499
                     /locus_tag="ACG81C_000841"
     CDS             23208..25499
                     /locus_tag="ACG81C_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5064023.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            25523..27604
                     /locus_tag="ACG81C_000842"
     CDS             25523..27604
                     /locus_tag="ACG81C_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064024.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            27621..27770
                     /locus_tag="ACG81C_000843"
     CDS             27621..27770
                     /locus_tag="ACG81C_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064025.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            27767..28213
                     /locus_tag="ACG81C_000844"
     CDS             27767..28213
                     /locus_tag="ACG81C_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064026.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            28215..>28310
                     /locus_tag="ACG81C_000845"
     CDS             28215..>28310
                     /locus_tag="ACG81C_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5064027.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT        10209 a         4229 c         6058 g         7814 t
ORIGIN      
        1 aaataaacat attagtccag ttttaaaaaa agagaagtaa tctatgtggg caaattctgt
       61 taatatgctt ttttgattta acacaatcaa actactgttg gacaaaaatg acagtaagga
      121 aagagttgct ccgattacta aataaaaaat agttgttaac aatattatat tatcaatttt
      181 tctgctcatg ttttcacccc accagaatta tatcaaaaag gagagaagaa ataatgcaag
      241 aattagtaat tttgaaaaat aaagaagctg tgactacgag cttacaagtt gcagaaagct
      301 tcgataaaaa gcacagacat gttttgacgg ctattgatga actaaaagag ggggttgccg
      361 aaaattgggc agacctattt tgggaagata cttatgttca tcctcaaaat aaacaatcat
      421 accgaattat ttatatgaat agagacggct tctctctatt agcaatgggc ttcactggta
      481 agaaagcttt aagttttaaa cttcaatata ttgaggcctt caacaagatg gaaaaagaat
      541 taaaagacca attgccatca attccagcaa ctaaacgaga aatggcatta cttgctctag
      601 ctgcaaacga agaaacgaat gaaagagtag acgctatcga gtctgattta aacgatttga
      661 agaacaatca acttctagct gaaccagatt accgaacaat ttctaatatg gttcgcaaca
      721 aaattagagt tatttgtaat cagcagcatt taaatagcaa agcaaaagca gaattgttta
      781 aagacttgaa tggcggaatt aagcgaatca ctggagcagt agctagaaat cgtatcaagg
      841 caaaacagtt cgatgatgtt atcgatttta ttaacaattg gatgccttct acagcgacga
      901 tgacaattat taagcaaatg gagttgttag aggatgaata gagctgaagc gctaaaaata
      961 ggcgtaataa ttgctaatcg ctggtggaga cacaataaac caaacatcct aagccaacaa
     1021 catattgata agcaaaaagc atggcaacaa ataaaaaagt gactcagccg accaaagcaa
     1081 tgagtcacaa agaaaataca tctaaggaga tgttaccaca tgaaaaaaga actttccact
     1141 ctagatcaat atttgactga tcctagttgg ggcaaatcga atatcaagga aacaagcaat
     1201 cgaaaaatca gacgtaatct tttgacggat gaagaactag catgtgatca agacgatttg
     1261 ggaaattttg tgagtatttg ggatcatgtc tatcttatcc atttatcgag gaagtccaaa
     1321 aaaactgaat atatctatgt catcgaagat ggcttgattg atgcgctaga agagtatgac
     1381 agagataact tgattgatat ctcttattac ggatcaggta agaaatacat tgctgaaatg
     1441 gaggcagaat ttgatgagtg aaaaggaaca acctttgaaa aatagaagtg ataatacgct
     1501 ttttaatacc ttatacaaaa taaatgtgaa agatgttact gaaaaacgaa ataacctcac
     1561 ttatctttcg tgggcatggg cttgggcaga agttagcaaa gtgtgtgagg cagtagatta
     1621 tgaaatctat catgatccag aaacatatct gccgtatgtc tttgataaga aaacaggcta
     1681 tatggttttt accagtatca cagtcaacgg agtaaagcgt gacatgtggt taccagtcat
     1741 ggatggtgca aacaaggcaa tgaaagatga gccatatacc tacgaagtca atgattatca
     1801 gtggaataac gaaacgaaga aaaaagagat tgttggaaaa atcgaaaagc gagttgaagc
     1861 agcaactatg tttgatatca acaaaacgat catgcgctgt cttgtaaaaa atctagcaat
     1921 gtttgggcta gggctatata tatttgctgg cgaagatatg ccagaagatg tctcgatgct
     1981 tgaaccagct actcaaagaa gcaaaaagct attcttggat gctttacaac tggttgctaa
     2041 caagtacgaa aaatcaattg atgaagcaat tgttgcattg actgatgcgg cttctataac
     2101 cgctgatgac agtaaatgga ccaagagaga cttgggcatt ctaaaacgag gcgttaactg
     2161 gattgaagat cagtacagag aagaaacaaa agagaagtga tatgagtgtt taaaccattg
     2221 atcgattcat attcagcggt tctgaaaaag ttcaaaggaa aagacatagg tgcaacgatc
     2281 aatgaagaag tgaacattga tcgactaaag acgatgtatg acggatatga tggcgatcgg
     2341 attattgaag ttcgatttat tgatccacgt cgatttactg tgcagcaacg aaacttcatc
     2401 tatgcgctga taggcgatat tttcatcgat acaggcatgc caacggactt ctggaaggaa
     2461 ttcttttact tccgttttga aggcgtcaca gggcgcaaaa taagcctcaa agacgaatcg
     2521 aatacaactg tgagtgatgc taatgtctta gcaaatatca tcttagattt catctttgaa
     2581 catcatattc ctttcaaaga aggctatgag attttaccag cgaatcaaga atattacttc
     2641 tacaaatgca tcacaaaaag agtctgctgc atctgtggca aaacaggagc tgacatcgat
     2701 cactttgaca aagcgctagg aagacgaaag cgcaaagaag ttgatcattc agagtacaca
     2761 tttgcagcac tctgcaggat tcatcacacg gagaaacaca atataggtgt gatcaatttc
     2821 aaaaataaat atcaaatcaa agggatcaag ttaaaccagg aaacaattaa gaaacttaga
     2881 ataggagggt aaaaaatatt gtctgacaac aaacgctact actatttaaa actaaaagag
     2941 aattttttcg atagtgacga gatggttctc ttagaaagta tgccagatgg ctatatttat
     3001 tctaatattc ttctcaaact ttatttaaga agtctaaaac acgaaggtaa gttgatgttt
     3061 aatgacagga ttccatttaa ctctacaatg ctcgcaacta ttacaagaca ttctgtagga
     3121 gtcgtagaaa aagcggtaca aattttccgt gatttgcagc ttattgacgt attagataac
     3181 ggagcaattt atatgtctga tatacaaagt ttcattggaa aatcctcaac tgaagcagat
     3241 agaaaaagag aatacagaaa gaaaatagaa gaggcaaaac ggaatttaat aactggagga
     3301 caagtgtcgg acaaatgtcc agacaaaact acaccagagt tagagataga gatagagaaa
     3361 gatatagata tagataaaga agaaaagaaa ggtaagtatt ctgacgaaca cttacgcctt
     3421 gctaaaaaat tgcaaagtaa tttaactgaa gattttccaa aagaaatgaa caaagtagat
     3481 atcgaaaaat gggcagacac aatcaggttg atggaagaaa gagataaagc gtctatagaa
     3541 gcgattgagt atgtgatcaa ttggctacct acaaatgaat tttggtttgg aaatattaga
     3601 agtgctaaga aattgagaga aaaatttgag aagctcaaat tcgaaatcaa agcagacaag
     3661 aataatcata aaaagcaaag tcaaaaacta cagtacagca atcctagtga atatgacgac
     3721 ttgccaattt aaaaaggaga tgcatcacat ggaaagccta gcaaatgcta tggagaaact
     3781 aataagaaga gtattagtgc aaagcgggaa atgtccagaa tgtagcgaac ctttgtatag
     3841 ttggcgagct aaaaataagg atggttcaga acgttgtaaa ccaacatgca tgagttgtgg
     3901 ttataaagcg ttacgtgtga aagaggatat acagaccgaa cggatatata acgacagctt
     3961 aaaagcacga gcgttgagtt tttttcaaaa tggttcggta ttaacagata aaactttgtt
     4021 taaatgcaaa atggagaatt atcacgtagt ggaccaagaa acgaaaattg ctttagaaag
     4081 agctaaaagc tttgtaaatg atgtcttact gaaccatcct gcacatttca ttctatcagg
     4141 gaaatcagga agcggaaaaa gccacttgtc aatggcgaca gcttgggaaa tacttgagcg
     4201 ctcaaattat gacaagaaaa tactttttat aagctatcaa gagttattag agcaaataaa
     4261 gttttcttat aacaatgctg aactgagaaa agaaattgaa ggatcgctta tagccgatat
     4321 taaaacaact gatttggtgg tttttgacga tattggagct gaattaggta gtggggtatc
     4381 aaatagtagg cagtttacaa acaacacgtt aaacacgctc ttagaagcaa gacagaacaa
     4441 ggcaacgatc atcacaacaa acttatctgg tcctgaacta agagaagcct acggtgaaag
     4501 aattgtttct aggatattta agaattcaga aggttatgcg ctgaaattcc agcaaacagc
     4561 agacaagcgc ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa
     4621 actgttcatc gaggtatcaa gtttgattct atcgcagaag cagagtatta cgatctagcc
     4681 ttgtggcaag ctgaagcgaa tggctggaaa gtaaaacttc aggaaagatt tgagctgatg
     4741 ccgaaatttg aactagacgg aaagaagtat cgcaagatcg agtatattcc cgacttcaca
     4801 ttttataaaa acggcaaact tgtcaaagtc gtagatgtta aaggaatgca gacaaaagac
     4861 tttaagatca aggcaaagct gttttgtcat caatatcaag tgccgttgat attagctaaa
     4921 aaatatcgga atacgttcaa ggaagagcgt ttttaaagag gtggtctatc atgacaacag
     4981 aagaagtgat tcaaatgcga attcgaagca ttcaacgtga gattgacgat ctggaacgaa
     5041 caaaggcagt gatggtcaat gaaacggcga gaaaggcaat cgatttgcac atagagaact
     5101 taagaaggga aattcgtaga ttggaggaat gagcgtggat aagaaagcag caatgaaacg
     5161 aatcatcgaa ctgacacatt ctgagaattg gcaagaagac aaagaaatag ttgcagaagt
     5221 ccaaaagctc ggtaaatcaa tgtggactga aaaaaccaga cggagaatgc cgagaaaaat
     5281 tgcaatctgg cacggtgacc gaattctagt aacaggtacc gctgaacagt tatctgaaat
     5341 tactggtctg agcaaaaaca tcatctggga tagagcgaag aatatggata ttgattctaa
     5401 aggaagacag tttaggtatg tggaggagaa ataatgaatc tcattacaca atacagtgat
     5461 atcatcctca agaaaatcat gatgaagatt cagaaagaca aaaaatcaaa agaacgagcg
     5521 gaattagtta agttggaaat ggctgaaaca ggggcaggag tgcgaagtag caggcattgg
     5581 aaggcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcaaatg
     5641 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
     5701 gtagagaaat ataaagagat attagacgaa tacatggagg gcagcaaatg ataccgagat
     5761 ttcgatcgtg gtacacacca tttaaaggta aaacaattgg acaagaaatg aaatatgggc
     5821 aagcaggaag gttgatcact catgctgaaa tggctccaga taaatatgtg cttatgcaat
     5881 ccacaggact gaaagataag aatggcgtag aaatatttga aggggatgta gttctggtta
     5941 atgtaagtaa tggttttgat catttagtta atgaaaaaac ggttgtacaa gaatccgagt
     6001 ttcattccgg attagtttgt aaatcattaa ttagtggaat ggaatataga atattcagcc
     6061 accaaaagat gggatacgaa tatgaagtca ccggaaatat acacgagaat agcgagttat
     6121 tggaggtaga gtgattatct tagctactga ctacatcgat tctttaaaag atgaaaatgg
     6181 gaatgtccca gcattgctaa attgtggaat taaaagtaag aatagaatca ttttgttacg
     6241 gatatcggct gatttagcaa aatatcttta tctgagcaag acagcagtat attacactgc
     6301 ggctacacgt tatcagtaca agggaaaaac aatcagtcag gattattatg atcgtttaat
     6361 cagtcctgac atacatggaa aatcaaaatc agcgatcaag ttatttggcg caatagagat
     6421 atatgccgat gattttccaa atttatggtt gaaggaggaa cagtaatgaa agactggtta
     6481 gaagaagcaa gcgccttagt tggctgtttc ataaactgtg cgtttatatc tgtaattatt
     6541 gcactggtgg gatttgtatc attgaaagtg attatattgc tttggcagtt aattttttag
     6601 gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt aaagtgatag
     6661 gaaatgatgc actagctgct tgctataacg agagtataaa tgcaggtatt acgttaatga
     6721 aaaaaataga cgaaccgaag aagctagtta ttccacaact cgtggccggt tggcttgaga
     6781 aatctacgga cccttttacg aaagctgaaa aaatagcgta tttaatcaaa tctaaagatg
     6841 gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg caagaacaag
     6901 gagaagaatt actcgcttgg gcaacgagac aatcatatga aacactattg agcttgtaca
     6961 acggctacga ggtcgagaaa gagccgttgt acagtgtaat aattgctggc gagtaccttg
     7021 tcaaggagat cagttgcagt aatgaagtta aactagtatc aacagcttac ctgccacaat
     7081 accttgcaca ccactaccaa ctgaccgaaa aacagatcaa agcaattgat gaaagatact
     7141 ggccatttgc tgtgccagtg gaagaggtgg cggaaggatg agtagacaga taatgctcca
     7201 tataccagac ggaacaaagg caatgacgat taccattgtt gctgcacaaa aagacggtgg
     7261 gttggcgttg aaaacaaaaa gcattgatac caaacagatt ttagaaggca gagatgtcga
     7321 gatagagatc gatgaggagg aagcggaatg aatgttcaag tttatttaaa aagagatttg
     7381 gccaggtttc ctcgaagctg ggatacaaat tcccacccat ccgaggcaga agaagagggc
     7441 tatctaatgt ctgctaacat gatagaaatt acaagagatg atgcggatga gtttgataaa
     7501 aaaagatcat ggtactcggt tccgaatcca atgtatacgg ctgtaataga agattatcat
     7561 atatccgaca gatttattct gattgatcta gaaaggccta agaaacgaat taaacagtat
     7621 agacgttgtg gattttcaac aaagaaatgg tgataacaaa acagaggtga agaaaaatga
     7681 acaacagaca ccgcagagta gcaaaactaa gaaatcaaga actgaatgta gcgaaagtaa
     7741 agttcgaacg ggagtatggt gtttctgcag aagaaacata taaagtggca agtcagtgtg
     7801 ttgctgatgc aagtgatgct attcgtaagt ttggaatttc gatactagat attaaatggg
     7861 aggatacaga atgaagattt atgtagtaaa gtttggaaat caattttaca gaagcgatga
     7921 acgttctata ggggctaaca cattatccat tgtagacata ctccaaagcg caagatggtt
     7981 tgataatctc gaagaagcta accaagtttc acgacgactt ggtggattaa cgcaagtata
     8041 tgaactggtc actgtcgatc atgagggggt agagtaatga aactaaaaga cggattttac
     8101 gctagtagtc atggtatcgg cggtttgatg ctagatatgc cgacaaagaa ccctaaaaca
     8161 cgtgagaaac caaaattcaa agtcggtgac atggttcgct gtgaagcaga aggatttatc
     8221 tatccatttc gtggatatgt agaaaaggta ttgtcgaact cagcaatcat tcgtattgaa
     8281 aacacgatgg aatgtgacaa gtggttagcg aaaagcaaag agaatttagc tgtagcgaga
     8341 ttagttgaca tggaagtaat cgagagcaaa taaaaaagcc ggatcgctcc gactaacata
     8401 ataaaacaga caagtttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
     8461 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
     8521 cgtttggagc ggatggcagg tcgctctttg atagatatta agtctccaat tattacggat
     8581 atgccgaagg cgccgaagca cggcaataag gcagaggacg cgatcattca gatgatggat
     8641 atagaagcag agagagatgc gattttagcg gctttgatgg ctcttagtct gattagccgt
     8701 cagatactct actacagctt ttgtgtgcca gatagcttct caaactacag aattagccgt
     8761 gaagtgggtt attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt
     8821 gcagaagcat ataaacacgg aagaataatt gcttataaat aatttggcgg ttttttggcg
     8881 gaatgatggc ggtttttagc tatttaccag tgatattatg gtagtgtcga aagattagtg
     8941 ataggtctga gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa
     9001 gcttcgatag acagcaacgg aaatattaag aataaggatg tgaatttcaa ctccttctaa
     9061 attgttctta ttatctatca tccgttgctg tctattgttt ttttaattat tcacacgata
     9121 actaaaggtg agtgaagaga aatgattcca ttaataattt caatttttgc gctctgtctt
     9181 aatgtctata tgattggatt taaaaatggg caaaataaaa aatagtagca gccaagaata
     9241 attttatagt gtcactgtgg cggaaagggt agacgcttaa aaataaggtc aatacgtcga
     9301 gggatagcct taacgtttta tgatttgacc atgcaatgtt cgattcattg ccagtgactt
     9361 tggcaaccga gggttggaaa tgggcgctca aagtacacga gcaaggcgag gtcgatagta
     9421 atcgatggaa tcggtgtagg ttgcttgata gagctataac tgcatctcgt ttatgaggtg
     9481 tagtttttac agattagatc actctttgag tggtcttttt attttacaca aaggaggtaa
     9541 caagaatgta tagaccacaa tacttagaac agaagtatga agtaatcact gtgcaaaacg
     9601 gtaacggtga gatagtacga aagtatagaa gaccaataaa gagcgataca tacaaacgaa
     9661 aggaaagcaa tgaagttatt ccattgtatg gcaaaagaat agctaagcat taaataagat
     9721 tgcgaaagga gacggaatat gaccgaggaa ttctatagat ggctattaca gttgataaga
     9781 gaagatcgtt tagttaagtt ctatcagtct cctaaatggc gcaggcttag agagaaagcg
     9841 atgaaacgag atcactatga atgccaagag tgtagaagac taggtaagta tcatagagta
     9901 gaaaacgttc atcatataaa ggaagtcaag gatagacctg acttagcttt agatttagat
     9961 aatcttattt gtttatgtgt tgaacatcat aatgaagttc atggcagata tcttacagca
    10021 ttagataaac aagagaagaa gatagaaagc tttgctaact tcgatgcaag tgaaaggtgg
    10081 taagtgcatg atcatcaatg ataatggcag agagtatgat acagaaaaga ttgaagagta
    10141 ttcatcttat actcagggat taattaaacg tttgatatac gttcgctatg tagatattag
    10201 ggatctgtta tcagataact gttgtagtaa atacaaagtg aatcaagtaa gagaagcgtt
    10261 gaataaagat aataacgtcg aaagaataaa aaatgttttt ggatatagta ttgaagagat
    10321 taattattac attgacttcg ctgaagcttt cattccgatg gtgagataac ccccccttaa
    10381 aataaatcgc aaattttttg ggggtgatga aacggagggg gctgtcagga aaagagattt
    10441 tttcgaactt tatcatgaaa ggagggctaa aatgtttaaa aacgaattgt ctcaaaatcg
    10501 ctacagagaa aaattacgcc gctctttaat aagccaattg gaaagtcaga aaacaaatat
    10561 tgagccattc ttagataatg ttgatcgtta tatcagttta tgggaaacgg cgatatcact
    10621 ggaagaagat atatccgaga acggcattag actggagaat ggtaaaaaga atgaatcagt
    10681 agcgttgctt gtttctgtca acaaacaaat gggattgatg ttggataaac ttgccattac
    10741 tcctgaattg gtaggtgaag caaatgaatc aattcctgag ttataagcat attgaaaatt
    10801 ggttcaaagc tatagaagaa ggcactatca aggtatgcaa agagcaatta ttgctaaaaa
    10861 attatctaga agaaagagtc tttactagag aagatattta cttcgataag cagatggtag
    10921 aggattcaat caatatacca gcacaatact ttccattcga attaattccg tgggaaaaat
    10981 ttctacaatg ttttatttat ggtgttcgat ggaaaaaaga taaaacacta gtgttcaata
    11041 gatatctttc attaatggga cgtggtaatg gtaaaactgg ttttgcttct tggaacaact
    11101 tctttctact aaccgctaaa cacggtatta aaaattatga tattgatatc tatgccaata
    11161 atgaaagcca agcaaagact agttttgatg atgtatttaa agtaattaaa gatcatcctg
    11221 atttagataa aaaagtattt aaagctacga aggaagttat tcaaaatatc gctacaaaca
    11281 gcaaacttcg ttataacacg gcaaatgcta gaacaaaaga tgggaagcga ccaggtgcaa
    11341 accgctttga tgaaattcac gaaaatgaag attattcaat gataaatgtg gctacttctg
    11401 gtggtggtaa aattcgagat tatagagaat tttatgatac aactaatggt catgttcgtg
    11461 gtggtccgct tgatgacatt atagaagaat caaaaatgat tctttctgga gaacttggaa
    11521 ttgacaagga tggagcagaa ttttctagtt tgtttccatt tatttgtcgc ttggataacg
    11581 ataatgaagt tgatgatccc gacatgtggg aaaaagcttg tccaactatt aattacaatg
    11641 cagatttaaa acggaaaatg tttcaagaat actctcaaat gcaacgtaat gctggtttaa
    11701 gacttacgtt catgaccaaa cgaatgaaca gacctatgga agatacacga tttgctgttg
    11761 cttcatatga tgatgttctg catacgaaag aaaaagaatt tcctgaaaaa atggatgaag
    11821 tgataggaac agtcgatttt gctgatagac gagattttgc cagcgttggg ttgctaggaa
    11881 aatacgataa agatgtttat tttacacaac atacttttat ccacgaatca gcccttcgat
    11941 tacaaaacat caaacgagag gttatagata tttctataga tcaaggaaaa tcacagatcg
    12001 ttcatggaaa aaatatagaa gctgattata ttgtaggttg gtttcttgaa atgagtaata
    12061 aatattatat taaaaaaatc gctatggata tgtaccgtgc aaaaatattg aagcccgctt
    12121 tagaagaagc aggttttact gtggaaattg ttcgaagcgg atctgttaca catggtatgt
    12181 taaaagatct ggttgatgac ctttttatta atcaacgttt attttttggt gacgatgcga
    12241 ttatgcgttg gtattgcatg aatgtatatg aagagcatat ttctaatgga aatatacgct
    12301 atgaaaaaat agaacctgaa actagaaaaa cggatggctt tttttcattc cttcatggtt
    12361 tgaatttttt agatgatatt tatgattctg ctcctgtaac agtcacaaat agctcagtag
    12421 aaaatacagg aactggattt actcctctag tattctaact tgaaaggagg tgagaaagtg
    12481 gggatttttc aaaaggcggt aggatacttc acaaaaaaag caacggttac tttagaagaa
    12541 tacttttgta aattgcaagt tgattttgtg tatcgaaaat ttgcgattga aacttgtatt
    12601 gatttgattg caaatgcgat gagtaaagcg gaattcaagt catatgaaga tggaaaaaat
    12661 aaaaagaatg atctttacta taggctgaat gtagctccta ataagaaaaa taatgcaaca
    12721 gaatttagaa aaaaactgat caggagatta atattctaca atgaagtatt gatcgtttct
    12781 ccgtctaata attctagcga aatatttatt gcggatagtt gggatgtcac agaatatgca
    12841 ttgaaagatg atgtgttttc tcaagtgcaa attaacaaca tagtccttga tagagaattt
    12901 ctagaaagtg atgttatcta tataaaatac gcagatcaac aaattaggca actagtcgat
    12961 gcgtattatc aagcgtatgg gaaactcatt tctagtgcca tgaatgttta caagcgctct
    13021 aacgctcgta gatacgtact gaaagggaat ttattccgac cgcaagacaa tacaacacaa
    13081 gatcaaatca ataaaatgat gacatcacaa tttaaggctt ttatggaagc tgataatgca
    13141 ggtgcggtat ttcaattaca aaatgagtac acattagaag atttcagcgg aaacttccaa
    13201 agcaattcaa gagatataaa aaacttaata gacgacatct ttgagatgac agcagcagcg
    13261 tttcacgttc cgaaaaacct actaaaggga gacatgagtg ggttatcgga tcaagtggac
    13321 gcttttttaa tgttcgaaat cataccgatt gctgaactta ttcaggatgc gtttaacgct
    13381 agtctctatg aagtagaaga atacttgtca gggaattttg tacgtgtgga tacaactatg
    13441 atcaagatta ctagcttcaa agatttggtt gacgctattg atgtaggcat tagaaatgga
    13501 gtatttacaa tcaacgaagg aagagaacgc gttggaaatg atcgctctga taaggcgatg
    13561 gcagatgaaa tatttataac taaaaacaat caacaagtat cgaaaggagg tgaggcgaat
    13621 gacgacaatg aaaacatttc tagcagtaaa gaatgaaggc gcagtaccgc aaatttttat
    13681 tcagggattt attggttcta gttggttctt tgaagggaat actgacaagg gaatcaaaaa
    13741 tattttggat agtctaggtg atcaagaaga aattgaagta gtaattaatt caaacggtgg
    13801 agacgtattt caagggattg ctattgggaa cttacttaag tcaaataaag caaaagttaa
    13861 cgttgtgatt aacggcttag ccgctagtgc tgcttcaatt atcgcaatgg ctggcgatac
    13921 tataaaaatc tacaacaatg cacaattgat gattcaccgc gcttccacat acggagaagg
    13981 aaatgtcgat gacttccgta cgattgctga ccaactggaa tcaattgata aatcggtaaa
    14041 ggcttcatat aaaacacgat tcaatggcac agatgaagca ttgcaagaac ttcttgaaaa
    14101 agaatcgttt atggatgcag aaacagcttt gagttatgga ttggtcgatg aaattatcga
    14161 tgcagaaaat agctcaggta ctgaagctaa aaaagaacaa agcgttgaag aaattttgaa
    14221 tgacgttaaa gaaaaaagag cagaaaaaat tgctgcattt acagcagcat taaataaaac
    14281 atttggacaa ggagatgtaa aataatgaca gttaaaaatt taaaaggtgt aacagctgca
    14341 agcgaccaat tgatgaaagc ttttaaagat ggtaacgaag aatcttttag cgcagctatg
    14401 gtaagcttat ctaaggaaat tcaggataaa attttagaag aagcaacagc aaaaaatcaa
    14461 gatcaattag tattaatgaa ccgtggtcag cgtgtattaa ctacacaaga aacaaaattc
    14521 tataacgaag tagtgaaaaa cgaaggtttt gcaggggtcg aagaattagt gccagctact
    14581 gtatttgaac gtgtatttga agatttagaa caatctcatc cactattgca aaaaattact
    14641 tttgttaaca caactggtgt aacagaatgg attgtgtcac gtggagtcaa tccagcatgg
    14701 tggggtaaac tttgcgaagc tgttaaaaaa gttttagata atggctttga tgtaattaac
    14761 atgaagcagt tcaagctatc aggttatatt cctgtatgta aggcaatgct tgatttaggt
    14821 ccagtatggt tagatcgtta tgtccgtact gttttagtag aatcattgag aattgcatta
    14881 gaacaagcaa ttgttgatgg tactggtaaa gatatgccag tcggaatgat gcgtgacatg
    14941 agcaaacaaa ctagcggaga atatgctgaa aaaaaagcag aacctattac agctttagat
    15001 gctgtaacta tgggcggttt gatggcgcga ctatcaaaat tcaatatcga aggtgtgaat
    15061 gatccgattt atcgtaatgt gaatccttct gatgtggtcc taattgtgaa tccaactgat
    15121 tactggtcta aagtattccc agctaagact gtactaactg ctaatggaga atacgtacaa
    15181 gtattgccag taccagtttc agatttgcag tcaacggctg tgccagaagg aaaagcagtt
    15241 attggggtag cctcagatta cttcatgggt gtaggatcta cactaaaaat tgaagcttca
    15301 gatgaatacc attttgttga agacgaacgc atttatctag ctaaacaata tgcaaacggt
    15361 caacctaaac gtaacgatag tttcattgta ttagatatta gcgctttggg aactactact
    15421 acaactacaa aaccaacaac cacaacaact acaacacaag cgtaggtgat cagaatgaag
    15481 tatattcttt gtcagccggc aatcaatcgg tttaaatggg agcttgaagt ttgtttaact
    15541 aatctgaaga aactaggaat caaagatatc gtattgcttt tcagcagaca cgatgatcag
    15601 attcctattt tttttgagaa ggaatatggt gttgaagttc atgtgtacga tgatctgcgg
    15661 gacgacaaag agtatattcc ttcgattaaa ccatatttat ggtggaaata tttagaagaa
    15721 gatcattcgc gtgaggacga ccgatatttc tatatcgatt cggatgtcat tttcaataaa
    15781 agaattaatt tgcgcaaatt gccttctaaa gatgatgttt ggtattgtag cgactgctgt
    15841 agttatctaa gtcttgatta tattagaagc tgtgaaaacg gagaaaatat tctaaaagat
    15901 atggcaaaca ttgtaaatgt tacagtagaa tctttggaaa ctataaacac taattcagga
    15961 ggcgcacagt gggttattaa ccgccctaaa gcgaattatt ggaaaaaggt ttatctggat
    16021 tctaatcggc tatatcgcta ccttagaggg caaaaaacaa atataaaaat ctggacagcc
    16081 gagatgtggg cacagctttg gaacatgatg tatttcaata ttggtcctaa agttcacgag
    16141 gaattagact tttgttttgc tactgatcca atagaaaaag ttaaagaagt aaaaatctta
    16201 cacaatgctg gagtaacaac aaacgatgaa gatttatttt tcaaagggag atacgtgact
    16261 tctacgcctt ttgatgaaga tttatcattt gtaaacaaga aaaaatgctc ttacgcatat
    16321 gttaaagcaa ttaaggcggt ggttagatga cgcctgaaca agtgactgaa gaattgctaa
    16381 tagctgtgaa ggataatatt tacgttacct ggaacgaaga agatgagtca attaaaaaga
    16441 tgatagctaa aaatgctgtt tatcttcaaa gtaaagtgag tacaacactt tctttttctc
    16501 ctgaaagctt agaatacgga ttgctaatcg aaagatgtag atacgactgg aatcgtgctt
    16561 tagatgagtt tgaacaaaat ttcgctagtg agttattagg tttcattcaa cattatgcgc
    16621 tacaagaata tattgcaggt gatgtgaatg gcgaataatc gtagactcga agaaacattc
    16681 aacgatggtt ggttaaagat tttgacgcaa actaccaaaa gaaatgaact aggaaaaaag
    16741 attggtgtag aagatacaga aatcacttct ttaaaattta gaaatctttc catgagagat
    16801 agtgatataa cagctatgga tgcgatggga tcgaaattaa ctaagaaagt aaagactcca
    16861 tttcatccaa tcgccaagaa atttaataaa gatcaatatt ttatcgtaat cgatagtatg
    16921 cgttacaacg ttatctatgc cgattacgat aatttttata tctattttta tcttgaaagt
    16981 gtgggtgaat atggtgattg ataattctaa agaaaaagaa cgtttaaata agcaaatttc
    17041 tgctatcaaa acttccttag aagagcattt tggcctcaaa ctctttcaag actccgtagg
    17101 cgaggatgag ctacctgatg attttaatta cttcattctc gaaacaggag aaatagaaat
    17161 gatcactgag ccaaaatata gcgtgggtca aaatctatat ctaactttct attcagaaaa
    17221 tagagaagat ttaacaggag attcactaga tattatttca ttgattcaaa atcgttcgat
    17281 tcgttttcag agaatggatc ccaatcattt aaaactagag aaccaagatc gctatatcga
    17341 tcaattggta tttacgttta gacgattatt gaagagtgat tgtcatggct aaaaatagtt
    17401 gggagctaaa aataaatgga catgatgaac ttcttgtgcg gatggaacgc tattcaagcg
    17461 agagcgaacg actgattaac gaagcattga aatcaaaagg ttcggctatt gcagtggata
    17521 ggattacaga aaaaattcct gtttctgaag cagatttaag aagagggcac caacacgcaa
    17581 aaaatagtcg tccacttaag actcaataca ttaatttggg tttcatcatt agacctacaa
    17641 gaaaatttga gtatttaaaa tatcctgatt tggggatagg tacttctaaa agaaatcagc
    17701 cggacgaatt tatgagaaga ggattaggtc ttgcacttga tccaattaca gaacttctga
    17761 ttcgtcaatt cgataaatta aataaatagg aggaacaaca atggctaaaa caacaacagt
    17821 aacaactttt gacaatataa gtattaaaag aatttctttt aattttaaga atgcaacaaa
    17881 tgcaatttca actgattgta atggacaatt agatggcgaa acagaaatgc agagaattgt
    17941 aaaaaaatgc ggttcaacag aagtaaaatc gaaatctaaa ccaatcaata tgacggtaac
    18001 aattactgca catgtaccga tggaagttta tcgacgcttc aatgggttga aacaagatga
    18061 acgtattaaa tcgggaattt actcttatgg tcctgattcc gtaggggaag atttctctct
    18121 tgctgcagag atcgtggatg atttcgaaga aaaaaataag ctgattggta tgttagcatg
    18181 cacttcgaat acaggattaa cattctctat tgaaaatggt gcggatgaag tagctgcgtt
    18241 agaactagaa acaaaagtta tgcaagatga atttggtaaa ttttatcatg aagcaattgt
    18301 tgcagaactt gaagaagact taacagatca atggatgacg aatctatctg ctgatgtgat
    18361 taaaaaaact tcagttgtga caactacggc cactcaatca cagtaaaaaa aacggaggta
    18421 gcgaaatgaa cgaagattac tcaaaaattg aactaaacga tggaacaatt ttgaatttag
    18481 aacctaaact gaatatcaag aaattattga tgatcaatag agattttaac acagacgagt
    18541 ttgcaaaaat gtcgatggga aaaggctcta tggatattac agttattcaa ggtgcaaaag
    18601 ccgtatatgt agcttatcgt caagcgaaca tggtcgatta catttcattc gatgaattta
    18661 tagataaatg agattttgat atggaggttg cagtagctgt atacagtact atgatgttca
    18721 aacaagcacg tgatgcctat caaaaagaat tcgaaaaagc aaataaggaa aaaaagcttc
    18781 aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc gatgtctatt cgatgttgac
    18841 cgacgttttt tctatgcctt cagatttggt tttaagcgat atctgtttag atgacatttt
    18901 gcaaatggct tacaacaaga gcgcttatga aggatggaag aactatgcaa taaatcaatc
    18961 ccagaaaaac taaaggaaag gaggtaaaaa atggctaaaa agagaacgga agcagaagta
    19021 actttcatag ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta
    19081 actaaaaata gagcagaatt aaaactagaa caagctcaat tgcaacagac tggttctgaa
    19141 tcagacaagt taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagtcaa
    19201 aaagttgaag taactagcca acgtttagcc aatgcgaaaa aatattatgg agaaaattcc
    19261 accgaagttc agaaacttga aagagaactg attaatcaac aaacagcgca acaacgtttg
    19321 tcaaacgaaa ttgataaaac gagtaatgca ctagctcaag caaaaggcga aatacagacg
    19381 tacgagtcta caatgcaaca gttggatagc gaacaaaaaa atgttcaagc tagtgcttct
    19441 ctgattgaat ccgaatacaa aaaatggcaa gcaactgctg gtcaatcagc ttctgaagcc
    19501 gagaaattag cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc ggagaaaacg
    19561 atagatatcc taagacgaca gttagaagct acacagtctg aatttggcgc tacatccaca
    19621 gaagcaatgc agatggaagc gaagcttaat gatgctgaac gtgaatttga agagttagga
    19681 caagctgcta aaaatgtaga tacaactaac ttggacgata tcggaagcaa aatagatatg
    19741 aataatttaa tggaagcttc tgacgtttta agcgacattg gcgataagct tacagaatta
    19801 gggaaacaag cagtggactc tgctaacagt gtaggtagtt cccagagtaa gatacaagct
    19861 aattttggtt tgtctaaaca agaggctgaa gaattaacga atgtagccag agacatttat
    19921 tataaaggtt ttggagaatc gttagatcag tccacagatg cattgatttt ggtaaagcgt
    19981 aatttaggag atttaaataa tcaagattta caaaatatca cggaacaagc tatggtccta
    20041 gaaaacacca tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc
    20101 aatttcggct tgagtgctca agatgcaatg gatttaatgg tttcgggtac tcaaaacggt
    20161 ttagataaaa cgcacgaatt aggcgacaat atggcagaat atagccaatt atggagtcaa
    20221 atgggatatt cagctgatga aacgttcgga atgcttcaaa atggtttaga tgcgggtgct
    20281 tataaccttg ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt aacagatggt
    20341 cgatttgagc aaaacatgga tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg
    20401 aaaaatggcg gaggaacaca aaaagacgtt attaattcca tgattcaaga ttttagcaat
    20461 atggatggtc aatacgacca attaaataaa gcttcgacaa tttggtctgc gcttggcgaa
    20521 gataatgcga tgaaagttgt ccaatcttta actgatgtta accatacatt tgatgatgtt
    20581 agtggatctg cacaaaaaat gaatgaagat tctactactc cgttacaaga attaaacgga
    20641 aaaatagctg aattaaagga ttcattagct cctataggca acacaatcat agatgcactc
    20701 gaaccagtaa ttgattttct aggaaagatg gctgatgcgt ttaataatct tccacaacca
    20761 gtacaggatt atgccgtagc gattggcgga ttgactgctg catttacttt attaatgcca
    20821 ataatagttg gcttcatggc tctaggtggt cctactacat taataatagg agcagttatt
    20881 actgctattg ctggagttat agcaattata aaaaactggg gcgcaattac tgactggttt
    20941 aagggaatat ggagtaaatt cactgattgg ttgggtggta cttgggaaag tataaaagaa
    21001 ggtgcctcat cagtttggga tggagttaaa gaaacctggt ctggatttgt agattgggtt
    21061 caagatattt ggcaaggagt ttctgattgg tttggagagc tatggagcgg attagttgaa
    21121 ggagcttcca acatctggca aggagtccaa gaaacttggc aaacattcgt tgattgggtt
    21181 tcaaatattt ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga
    21241 attgttcaaa taccatggac attaataacg tcattgattc aagctggtat taatattatc
    21301 gtgggtattt ttgatgtagc tggacagtta ttaggcgcag cttggcaagc tgtttggaca
    21361 cctatttctg atttccttaa aaatacttgg gatactatga cacaatggat aagcatcgct
    21421 tggaacggaa ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat
    21481 ggtatatgga catctattag tactacggct tcaaatattt ggaattcaat tagtgcaaca
    21541 gcttctagta tttggaacag tatcaagaat acaatcacta gcttggtaca agcagctgct
    21601 acagtaattc aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca
    21661 atcagctcta cagcatcaaa tatctggaat agtgtgacta gtagtataag caatgctata
    21721 aacgcagcta aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gatcagcgga
    21781 atttggaacg gtatcaaaaa cactgctttg aatctttgga atggaattac aagcactatt
    21841 agctctaaag taaacgatgg aaaaaatgca atttcaagcg gttggtccaa tctaacaggt
    21901 attgtttccg acatattcaa taatgttaaa agtacaattg ctaacatttg ggaaggtatc
    21961 aaaaagactg ttagcgctcc gattgattgg attagagata aaatcagtgg catctttgat
    22021 aatttgaata tttcgatacc acatattccg ttaccacatt ttaaattgag tggggaattc
    22081 aatccattga agggaaaaat cccaacgttg ggtgttgatt ggtatgcgaa aggtagtgtg
    22141 tttaattctc cgaatattat cggtgtcggt gaagcaggac ctgaagcagt tttacctttg
    22201 aaaagatctg ttttgcaaga aattggtgat cgtatcttga gtagcacatc agtttcatct
    22261 agggcacaaa cgattcaacc tgtgaataac tacgaattca atttcacaat tgatggtaac
    22321 gcagatgagg ttactatgaa gcaaacaact caacaaatca ttgatagcat tacaaaaatt
    22381 caaaatgata atgcttcggc atggcgttaa acaggagagt atttctcctg tttttttagt
    22441 attaaaaagg atgtgaaaaa atgactgatt gtatacattc tataatcgat ggatttcctg
    22501 attatttgca taaattggct ttagctgaaa gaccaaccat accttctcca aaaagacaga
    22561 gagttgaaac ttctgtttta ggtaggttag gtggcttagt acaagattac tcgtttgaag
    22621 atatgtcgtt tacattgcac tataactatt tagaggatgt ggaagaccat caagcgttca
    22681 agcaatcgtt ttatatcatg cgtcattggt taaactatgc aaagaaatta gaattctctg
    22741 acgatcccaa cgtctattac gttatccaga ctatcgatat tggggatgca gaaaacgata
    22801 ttgttgaatg gggagagttc gatgtaaata ttactgcgaa accattcgca agagttcaag
    22861 aagatgtacc tataaccgta gataaaccac agtcatttaa cttgctgaat aatagtttag
    22921 aagaaagttt tccaaagatt atcatcactc cttcagctac ttcatgccag ttcatcttaa
    22981 atgattatgt gtttagtttc gaaggcttag tagcaggaac tgatgtagtc attgatagtg
    23041 atttgatgct ttgctacgaa gagcaatcgg acggagatat tttagatcgg tccaacaaaa
    23101 tgaagaccat gcaatatcca acgttgcaag tggatattaa tcattttaat tgtactggtt
    23161 tgagcaaaat acaaatttat cgtaacgggt taaggtaggt gaaatagatg atcgataatt
    23221 taataactat ttacgataaa aatgacgcga ataatttagc tgaacattta tatgatacgc
    23281 aaggtttagg cgctttgtca gactggttaa cagctactgt tagcaataaa ctaaacggag
    23341 ccgagatatt tcagggtact tattcaataa acggaactaa tgcagatttg attgtagaag
    23401 gacgtattat tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta cggatttatt
    23461 atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa agctgaacct attttcaatg
    23521 atataagaaa atcggtgttg aataaatatg acagcggaac agaaaagatc actgctactc
    23581 aggcatggca aaacgcaaaa gttttagcga aaccagctat cccttcgcag ttttctttct
    23641 cgtcattagt agatacgctt gctaatgtga agatagaaaa ggcgaatttt ttagaattct
    23701 ttggtggaaa agagggatct attctagatc gatttcatgg tgagtttcta aaagataata
    23761 acacattacg tcatgaaaaa agtctaggta cggatcataa aatcaaagcg atttatacta
    23821 aaaacttaac tggtcttgac ttagagattg atgctcaaag tgttttagtt ggagtttatc
    23881 cattcattag cagctcttca gaaggagaag acgagatcac tctaccagaa gaagttattt
    23941 tcacggatta cgtggatgat tatcctgctg gatatgtttc ttttgttgat tttaaagaca
    24001 aagcgactga tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa acaaacatag
    24061 ataaacaaaa accacaagtg agtggttcga ttgaattagt accattgagg catcaaagag
    24121 gctatgaaaa atttgttgat ctagaaaaag tttcgatggg tgacggagta gatgtgtatc
    24181 atccacagtt aaaagtgaat atgtcagcga gaattgtgga atatacgttt aatgttctaa
    24241 ccaattcata cgataaatta gttgtaggaa acgtcaaaac aaacttctta gaaaacacag
    24301 agaataatgt cagcaatttg attaatgatg ccattgatca attgaaaaat ggtggcgaaa
    24361 tcagtgattt aatcaatgat attgtagatc atcaaactga tatgattact ggtcaaaatg
    24421 gtggttacgt tttattagat cctaaagaag cccctagtcg tattttgatt atggacacac
    24481 cagataagaa tactgcacgg aacgttttgc aaatcaacaa cgctggtatt ggtttctcta
    24541 aaactggcat taatggaacg tatgacaccg catggacgtt agatggcgga ttcaatgcct
    24601 cgtttattac agctggtgag atagtaggga ttactattag aggtactaca ttaattagtg
    24661 atggcactga ttatagaaca agtatagcta atggcaaaat gacttggtac tcaaaaaaag
    24721 ttaacaaaga tattatggag ctagaagcac gtgattatgt aagtgctgat gcaggtattg
    24781 tatcatacac catgaaaact ggtggtggtt tcatgattag aaatccacag gggaatttgg
    24841 tttttagtac gtgggataat ggtaataaca aaccgttttt atcttttggt gcgcccaatt
    24901 tcaggtatag caatgctagt tatataactg atggcgatgg tagttcttta agcattaatg
    24961 gtagtgcggg cagttcatgg gagtttaaag tagctggtag gactatgaaa tttactagtg
    25021 atggtatgct aacgttgcca ggttgttttt ttggttcatg ggaagatgga aaacttgcta
    25081 ggtttgaaca atcaacggta caagtatata aaaattttac tgttagaggt actaaaaact
    25141 caaccgtacc aacagaacat tatggacaac gactattaaa cgcttatgaa actccagaat
    25201 attatttcgc tgattatggg gaagccgtta caggtgatga tggtaaagtt cgtgttgata
    25261 ttgatcctat gtttgccgag acagtaaatc taagtcgata tatgacacat gtgacaccta
    25321 cagagctagt tttgtgtgct gttactcatg aagatattga ccatttcatc attgaaacta
    25381 gtaagccaaa cgtattggtt agatggaatt tagtggcaca tcgactaggg tatgaagatg
    25441 ttagattaaa agaggataca gcatatgata gcacagtgct tgaccaaaaa cgtttttaaa
    25501 acgaagacaa ggaggtatat aaatggctag cagtttatat aatttggctt tagatttcag
    25561 caaagaatta aactacacca aagctattat ggctcgtcaa ggtgacaaag gaattacggt
    25621 gactgttaaa ccgtttttaa atggcttgca gatggatacg agtggcggaa cctttacatt
    25681 aaaaggaaca acaccatcta accgttacgt agatagtgtc gctactagta taactagcga
    25741 agaagtcaca ttttctcttg atggaacatt tatgagtgaa gcaggatact ataaacactg
    25801 ctacgtagaa tatagaaaag ataatcaaat tttaacgacg caagatatca tttttttctc
    25861 actaggagtg tctgacattt cgcaaggcca agccgatgaa tatgtttcgc aattagaaga
    25921 attgattcga aagtataacg aaacttttga tgcttttctg gctgaaatta aaggtagaat
    25981 ggatagctta aataaacaga ttactgattt aactggtcaa gctaaaacac tacaagacaa
    26041 gttagatgct ctgaaagaag aaatttctaa attaggtaac ttacatgtga tgtactccaa
    26101 cagcatcgac ttcgggggct atgattatag tgggaatccg aatttaatgt ccaaactaaa
    26161 atcgagcgat tttaacgttg gttaccacgg gtcactaact aatgttaacg gaatgttaca
    26221 ttttatgtct gatggtacag gggacattga tatgtttacg cgtattaata cacctcagct
    26281 tgctagtggg aaaacctata ctctgagtgc gaaagttcga tttgatgaag gaactacagg
    26341 agctattgat aaattacgtt tggtgtatcg tacatcacca ggagaaaaga tattattgga
    26401 agcaaatagt acaaatatta caacagatga tgtagggaaa gaaataacaa tcaaaggtac
    26461 agctaacgtt aattatcaaa tcacaaattt agatcgattt tatattagtg ttagtttttc
    26521 taacagggat aaaataaatg gtggatttaa attgtacgac atcaaaatcg aagaaggctc
    26581 aacagccact ccgtatcagc caaacttact agatgaccct tactgggtag gtaaaacatc
    26641 gttgggtaag aatattgcaa ataaaggtac gacgttccca attaaatcta gcgcgtacga
    26701 aatatataaa ggtaacacgg aagaagagct tataataggt caaacgtata ctatcacgct
    26761 taaaggaaca aaacccgcaa gtcaaacctt tgtagcatat aattattgga acgttaattt
    26821 tggagatcta aaaccagttg agggattgac agacgtatgg tctctaacat tcacaccaac
    26881 gaaacttgaa ccgggtttgc ctaaagacct tcgcattttt cagtcgccta aagaaacggc
    26941 cggcgcatgc cgaattgact ggctcaagat cgaaaaaggc gacacacgaa ccccgaatat
    27001 tgagcaatat aaataccgag gaatcggcat gcgagactca aacaatccaa aagattatgt
    27061 atgggatcta gaacctagct atgttgagaa taacctagct acagatatca aggttgctga
    27121 aatcacaggt aaagcaaaca attataccga tgggaaagta tcggagatta attcgcagtt
    27181 gactgcttca attaatgaag tagacaccac agctaaggat gctcaaacaa aagcgaatgc
    27241 taatgcgact gctatagatg aattagacaa taagatcgat gaacgcatta atgatacagc
    27301 tactaccaca ttaacagtta caaacgggaa taccggatca gcaaagcttt atcgtgaagg
    27361 aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa cgcagtggtg gaaatgattc
    27421 aacgatacta acaattccag aaggctatcg gccaccaatt agttttgagc aactggttgg
    27481 ctcgatagac cgttctactt tgaacagtgc tcagttatct attggtgcag atggagccat
    27541 taaatggcga agaaactcaa gttatggatc ggattatacc tttgcaatta cttacacgat
    27601 ttagaaaagc gtgaatcgat atgaaggcag catatagacc aattgaacct tacggattcg
    27661 agcaaatcat tgtgaatgat gaagaacatt taccggaaga atgcacagaa gtcgaaccac
    27721 cgattccaaa ttggaaaccg aaattcaatt actgggaggg aaataaatga aaaacatttg
    27781 gaaatatgga cgtactggcg gagagtacgc aggaaaagta ttggacgaca tgcttgtatc
    27841 cgttccttac acagatcagc caccgcttga aggaattcgt gctgatggcg aaccgttaac
    27901 aatcgctgat caaatgtttg atcctaaatt gaatcaatgg attattttag cgaacgcact
    27961 agatcacaac gatttaaaca atctcaaagc aatgtatgag tcgttagaaa atgagaacgg
    28021 cgatttaaaa cagatcaatg ccaaactcat gctaagcgat gtagcaatta aacaggaaaa
    28081 tactgcattg aaagaaaaag cggatagttt agcacaaatc aattcaaaaa tgatgcttgc
    28141 ttcgttacaa aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa
    28201 gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    28261 tcactaacga acaagtaatg gttttcgttc cgttgtgcat tactgaagaa
//
LOCUS       DAZUMI010000022        28077 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000022 DAZUMI010000000
VERSION     DAZUMI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.022"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..262)
                     /locus_tag="ACG81C_000846"
     CDS             complement(<1..262)
                     /locus_tag="ACG81C_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5064028.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTA"
     gene            complement(264..461)
                     /locus_tag="ACG81C_000847"
     CDS             complement(264..461)
                     /locus_tag="ACG81C_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5064029.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(855..2582)
                     /locus_tag="ACG81C_000848"
     CDS             complement(855..2582)
                     /locus_tag="ACG81C_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5064030.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2575..3768)
                     /locus_tag="ACG81C_000849"
     CDS             complement(2575..3768)
                     /locus_tag="ACG81C_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5064031.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3955..4599)
                     /locus_tag="ACG81C_000850"
     CDS             complement(3955..4599)
                     /locus_tag="ACG81C_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5064032.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4693..4827)
                     /locus_tag="ACG81C_000851"
     CDS             complement(4693..4827)
                     /locus_tag="ACG81C_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5064033.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="ACG81C_000852"
     CDS             complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="ACG81C_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5064034.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6958..7431)
                     /locus_tag="ACG81C_000853"
     CDS             complement(6958..7431)
                     /locus_tag="ACG81C_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5064035.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7832..8056
                     /gene="nrdH"
                     /locus_tag="ACG81C_000854"
     CDS             7832..8056
                     /gene="nrdH"
                     /locus_tag="ACG81C_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5064036.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8215..10374
                     /gene="nrdE"
                     /locus_tag="ACG81C_000855"
     CDS             8215..10374
                     /gene="nrdE"
                     /locus_tag="ACG81C_000855"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5064037.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10424..11389
                     /gene="nrdF"
                     /locus_tag="ACG81C_000856"
     CDS             10424..11389
                     /gene="nrdF"
                     /locus_tag="ACG81C_000856"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5064038.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11478..12617)
                     /locus_tag="ACG81C_000857"
     CDS             complement(11478..12617)
                     /locus_tag="ACG81C_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5064039.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12926..13639)
                     /locus_tag="ACG81C_000858"
     CDS             complement(12926..13639)
                     /locus_tag="ACG81C_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5064040.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="ACG81C_000859"
     CDS             complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="ACG81C_000859"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5064041.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14828..16360
                     /locus_tag="ACG81C_000860"
     CDS             14828..16360
                     /locus_tag="ACG81C_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5064042.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16551..17753)
                     /locus_tag="ACG81C_000861"
     CDS             complement(16551..17753)
                     /locus_tag="ACG81C_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5064043.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17835..18524)
                     /locus_tag="ACG81C_000862"
     CDS             complement(17835..18524)
                     /locus_tag="ACG81C_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5064044.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18630..18998)
                     /gene="rplL"
                     /locus_tag="ACG81C_000863"
     CDS             complement(18630..18998)
                     /gene="rplL"
                     /locus_tag="ACG81C_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5064045.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19051..19554)
                     /gene="rplJ"
                     /locus_tag="ACG81C_000864"
     CDS             complement(19051..19554)
                     /gene="rplJ"
                     /locus_tag="ACG81C_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5064046.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19758..19885)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19964..20653)
                     /gene="rplA"
                     /locus_tag="ACG81C_000865"
     CDS             complement(19964..20653)
                     /gene="rplA"
                     /locus_tag="ACG81C_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5064047.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20757..21179)
                     /gene="rplK"
                     /locus_tag="ACG81C_000866"
     CDS             complement(20757..21179)
                     /gene="rplK"
                     /locus_tag="ACG81C_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5064048.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21522..22190
                     /gene="sdaAB"
                     /locus_tag="ACG81C_000867"
     CDS             21522..22190
                     /gene="sdaAB"
                     /locus_tag="ACG81C_000867"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5064049.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22203..23075
                     /gene="sdaAA"
                     /locus_tag="ACG81C_000868"
     CDS             22203..23075
                     /gene="sdaAA"
                     /locus_tag="ACG81C_000868"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5064050.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23166..24266)
                     /locus_tag="ACG81C_000869"
                     /pseudo
     CDS             complement(23166..24266)
                     /locus_tag="ACG81C_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24358..25245)
                     /locus_tag="ACG81C_000870"
     CDS             complement(24358..25245)
                     /locus_tag="ACG81C_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5064051.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25322..25867)
                     /gene="nusG"
                     /locus_tag="ACG81C_000871"
     CDS             complement(25322..25867)
                     /gene="nusG"
                     /locus_tag="ACG81C_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5064052.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25968..26138)
                     /gene="secE"
                     /locus_tag="ACG81C_000872"
     CDS             complement(25968..26138)
                     /gene="secE"
                     /locus_tag="ACG81C_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5064053.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26162..26314)
                     /gene="rpmG"
                     /locus_tag="ACG81C_000873"
     CDS             complement(26162..26314)
                     /gene="rpmG"
                     /locus_tag="ACG81C_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5064054.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26476..27120
                     /gene="cbpA"
                     /locus_tag="ACG81C_000874"
     CDS             26476..27120
                     /gene="cbpA"
                     /locus_tag="ACG81C_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5064055.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27176..27679)
                     /locus_tag="ACG81C_000875"
     CDS             complement(27176..27679)
                     /locus_tag="ACG81C_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5064056.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27692..27738)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         8498 a         5802 c         4965 g         8812 t
ORIGIN      
        1 cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc
       61 ttattttatt agtataagca gtaaaatata gataattcac caatctattt attaaaggac
      121 cgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
      181 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
      241 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
      301 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
      361 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
      421 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
      481 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
      541 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
      601 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
      661 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
      721 gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
      781 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
      841 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
      901 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      961 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
     1021 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
     1081 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
     1141 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
     1201 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
     1261 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
     1321 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
     1381 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
     1441 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
     1501 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
     1561 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1621 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     1681 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     1741 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     1801 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1861 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1921 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1981 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     2041 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     2101 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     2161 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     2221 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     2281 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     2341 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     2401 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     2461 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     2521 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     2581 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     2641 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     2701 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     2761 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     2821 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2881 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2941 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     3001 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     3061 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     3121 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     3181 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     3241 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     3301 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     3361 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     3421 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     3481 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     3541 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3601 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     3661 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     3721 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     3781 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3841 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3901 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3961 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     4021 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     4081 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     4141 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     4201 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     4261 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     4321 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     4381 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     4441 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     4501 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     4561 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4621 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     4681 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     4741 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     4801 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4861 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4921 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4981 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     5041 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     5101 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     5161 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     5221 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     5281 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     5341 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     5401 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     5461 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     5521 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     5581 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     5641 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     5701 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     5761 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     5821 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5881 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5941 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     6001 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     6061 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     6121 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     6181 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     6241 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     6301 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     6361 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     6421 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     6481 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     6541 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6601 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     6661 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     6721 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     6781 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6841 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6901 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6961 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     7021 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     7081 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     7141 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     7201 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     7261 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     7321 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     7381 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     7441 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     7501 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     7561 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7621 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     7681 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     7741 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     7801 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7861 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7921 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7981 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     8041 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     8101 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     8161 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     8221 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     8281 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     8341 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
     8401 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     8461 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     8521 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     8581 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     8641 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     8701 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     8761 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     8821 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8881 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8941 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     9001 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     9061 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     9121 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     9181 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     9241 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     9301 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     9361 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     9421 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     9481 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     9541 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9601 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     9661 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     9721 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     9781 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9841 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9901 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9961 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
    10021 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
    10081 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
    10141 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
    10201 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    10261 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    10321 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    10381 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    10441 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    10501 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    10561 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10621 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    10681 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    10741 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    10801 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10861 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    10921 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10981 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    11041 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    11101 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    11161 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    11221 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    11281 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    11341 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    11401 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    11461 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    11521 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    11581 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    11641 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    11701 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    11761 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    11821 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11881 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11941 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    12001 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    12061 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    12121 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    12181 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    12241 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    12301 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    12361 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    12421 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    12481 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    12541 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12601 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    12661 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    12721 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    12781 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12841 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    12901 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    12961 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    13021 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    13081 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    13141 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    13201 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    13261 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    13321 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    13381 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    13441 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    13501 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    13561 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    13621 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    13681 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    13741 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    13801 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    13861 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    13921 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    13981 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    14041 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    14101 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    14161 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    14221 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    14281 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    14341 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    14401 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    14461 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    14521 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    14581 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    14641 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    14701 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    14761 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    14821 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    14881 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    14941 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    15001 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    15061 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    15121 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    15181 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    15241 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    15301 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    15361 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    15421 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    15481 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    15541 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    15601 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    15661 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    15721 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    15781 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    15841 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    15901 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    15961 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    16021 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    16081 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    16141 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    16201 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    16261 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    16321 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    16381 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    16441 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    16501 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    16561 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    16621 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    16681 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    16741 ttgcttgctg cttcacctag cccttgtact cgtgtttgat tggcgacttg accaaattga
    16801 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    16861 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    16921 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    16981 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    17041 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    17101 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    17161 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    17221 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    17281 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    17341 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    17401 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    17461 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    17521 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    17581 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    17641 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    17701 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    17761 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    17821 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    17881 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    17941 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    18001 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    18061 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    18121 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    18181 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    18241 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    18301 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    18361 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    18421 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    18481 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    18541 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    18601 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    18661 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    18721 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    18781 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    18841 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    18901 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    18961 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    19021 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    19081 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    19141 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    19201 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    19261 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    19321 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    19381 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    19441 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    19501 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    19561 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    19621 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    19681 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    19741 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    19801 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    19861 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    19921 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    19981 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    20041 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    20101 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    20161 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    20221 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    20281 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    20341 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    20401 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    20461 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    20521 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    20581 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    20641 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    20701 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    20761 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    20821 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    20881 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    20941 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    21001 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    21061 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    21121 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    21181 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    21241 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    21301 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    21361 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    21421 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    21481 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    21541 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    21601 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    21661 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    21721 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    21781 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    21841 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    21901 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    21961 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    22021 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    22081 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    22141 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    22201 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    22261 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    22321 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    22381 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    22441 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    22501 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    22561 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    22621 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    22681 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    22741 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    22801 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    22861 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    22921 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    22981 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    23041 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    23101 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    23161 ttttattaat ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt
    23221 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    23281 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    23341 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    23401 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    23461 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    23521 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    23581 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    23641 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    23701 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    23761 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    23821 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    23881 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    23941 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    24001 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    24061 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    24121 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    24181 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    24241 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    24301 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    24361 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    24421 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    24481 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    24541 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    24601 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    24661 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    24721 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    24781 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    24841 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    24901 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    24961 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    25021 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    25081 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    25141 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    25201 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    25261 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    25321 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    25381 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    25441 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    25501 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    25561 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    25621 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    25681 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    25741 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    25801 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    25861 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    25921 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    25981 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    26041 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    26101 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    26161 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    26221 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    26281 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    26341 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    26401 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    26461 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    26521 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    26581 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    26641 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    26701 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    26761 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    26821 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    26881 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    26941 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    27001 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    27061 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    27121 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    27181 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    27241 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    27301 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    27361 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    27421 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    27481 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    27541 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    27601 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    27661 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    27721 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    27781 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    27841 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    27901 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    27961 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    28021 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggctt
//
LOCUS       DAZUMI010000023        27576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000023 DAZUMI010000000
VERSION     DAZUMI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.023"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG81C_000876"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG81C_000876"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5064057.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG81C_000877"
     CDS             complement(937..1518)
                     /locus_tag="ACG81C_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5064058.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG81C_000878"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG81C_000878"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5064059.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG81C_000879"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG81C_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5064060.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG81C_000880"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG81C_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5064061.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG81C_000881"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG81C_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5064062.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG81C_000882"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG81C_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5064063.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG81C_000883"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG81C_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5064064.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG81C_000884"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG81C_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5064065.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG81C_000885"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG81C_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5064066.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG81C_000886"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG81C_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5064067.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG81C_000887"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG81C_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5064068.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG81C_000888"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG81C_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5064069.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG81C_000889"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG81C_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5064070.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG81C_000890"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG81C_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5064071.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG81C_000891"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG81C_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5064072.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG81C_000892"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG81C_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5064073.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG81C_000893"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG81C_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5064074.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG81C_000894"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG81C_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5064075.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG81C_000895"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG81C_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5064076.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG81C_000896"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG81C_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5064077.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG81C_000897"
     CDS             13972..14157
                     /locus_tag="ACG81C_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5064078.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG81C_000898"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG81C_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5064079.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG81C_000899"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG81C_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5064080.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG81C_000900"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG81C_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5064081.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG81C_000901"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG81C_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5064082.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG81C_000902"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG81C_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5064083.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG81C_000903"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG81C_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5064084.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG81C_000904"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG81C_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5064085.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMAIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG81C_000905"
     CDS             18232..18879
                     /locus_tag="ACG81C_000905"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5064086.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG81C_000906"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG81C_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5064087.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG81C_000907"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG81C_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5064088.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG81C_000908"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG81C_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5064089.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG81C_000909"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG81C_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5064090.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG81C_000910"
     CDS             20317..21255
                     /locus_tag="ACG81C_000910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5064091.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG81C_000911"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG81C_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5064092.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG81C_000912"
     CDS             21935..22774
                     /locus_tag="ACG81C_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5064093.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG81C_000913"
     CDS             22750..23619
                     /locus_tag="ACG81C_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5064094.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG81C_000914"
     CDS             23609..24406
                     /locus_tag="ACG81C_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5064095.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACG81C_000915"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACG81C_000915"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5064096.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26019..26213
                     /locus_tag="ACG81C_000916"
                     /pseudo
     CDS             26019..26213
                     /locus_tag="ACG81C_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26401..27177)
                     /locus_tag="ACG81C_000917"
     CDS             complement(26401..27177)
                     /locus_tag="ACG81C_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5064097.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            27300..>27576
                     /locus_tag="ACG81C_000918"
     CDS             27300..>27576
                     /locus_tag="ACG81C_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFS5064098.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLI"
BASE COUNT         8965 a         5054 c         5823 g         7734 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggccatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact aagtacttta
    26341 ttttactctt taggattgat acagtagcat ctatcccttt attaataatc ttttattttt
    26401 ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa ttcctttttc
    26461 tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag cttccatatg
    26521 aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat cgtctttccc
    26581 cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta ctatgatttc
    26641 tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat ctcctgtaaa
    26701 gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac ctacataatt
    26761 caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag attcaaaaac
    26821 tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt cattcgcatc
    26881 attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg ctatatcgtc
    26941 ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga taatatcttc
    27001 gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg atggaaaagg
    27061 acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat caattaaaaa
    27121 ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta tattcataaa
    27181 cgttgtctcc tttttgttga tatgaaatat ttttagtata attcaattcc atacaataaa
    27241 taaaccgaac aaaaaaagtc ttttactatt tttacttaat ttatgaaagg ttgtttacca
    27301 tgttagacca aacagatata aatatcattg aggaactatc aaaaaatagt cgaatcacaa
    27361 tgaaagaact tggagaaaaa gtacatttga ctggacccgc tgtctcggcg agagtgacaa
    27421 aactagagga aagtggagtt attgaaaatt atacagtaaa attaaatatg gaaaagctag
    27481 ggttttcgat tcacgctttt ttaattataa ttacgcaaaa tttaaatcat aaaccctatt
    27541 tatcatttat taaaaatcat gaaaaattct tgatta
//
LOCUS       DAZUMI010000024        27557 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000024 DAZUMI010000000
VERSION     DAZUMI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.024"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(271..363)
                     /locus_tag="ACG81C_000919"
     CDS             complement(271..363)
                     /locus_tag="ACG81C_000919"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5064099.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
     gene            complement(634..1614)
                     /locus_tag="ACG81C_000920"
     CDS             complement(634..1614)
                     /locus_tag="ACG81C_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5064100.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1627..2505)
                     /locus_tag="ACG81C_000921"
     CDS             complement(1627..2505)
                     /locus_tag="ACG81C_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFS5064101.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2521..3321)
                     /locus_tag="ACG81C_000922"
     CDS             complement(2521..3321)
                     /locus_tag="ACG81C_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS5064102.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3596..3745)
                     /locus_tag="ACG81C_000923"
     CDS             complement(3596..3745)
                     /locus_tag="ACG81C_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064103.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            4713..5039
                     /locus_tag="ACG81C_000924"
     CDS             4713..5039
                     /locus_tag="ACG81C_000924"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5064104.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5047..5955
                     /locus_tag="ACG81C_000925"
     CDS             5047..5955
                     /locus_tag="ACG81C_000925"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5064105.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6581..6835
                     /locus_tag="ACG81C_000926"
     CDS             6581..6835
                     /locus_tag="ACG81C_000926"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFS5064106.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            7377..7535
                     /locus_tag="ACG81C_000927"
     CDS             7377..7535
                     /locus_tag="ACG81C_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5064107.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7607..8833
                     /locus_tag="ACG81C_000928"
     CDS             7607..8833
                     /locus_tag="ACG81C_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5064108.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(8922..9314)
                     /gene="rpsI"
                     /locus_tag="ACG81C_000929"
     CDS             complement(8922..9314)
                     /gene="rpsI"
                     /locus_tag="ACG81C_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5064109.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(9330..9758)
                     /gene="rplM"
                     /locus_tag="ACG81C_000930"
     CDS             complement(9330..9758)
                     /gene="rplM"
                     /locus_tag="ACG81C_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5064110.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10292..10759)
                     /locus_tag="ACG81C_000931"
     CDS             complement(10292..10759)
                     /locus_tag="ACG81C_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5064111.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10879..12417)
                     /locus_tag="ACG81C_000932"
     CDS             complement(10879..12417)
                     /locus_tag="ACG81C_000932"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5064112.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            12497..13183
                     /locus_tag="ACG81C_000933"
     CDS             12497..13183
                     /locus_tag="ACG81C_000933"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5064113.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(13384..13911)
                     /gene="msrA"
                     /locus_tag="ACG81C_000934"
     CDS             complement(13384..13911)
                     /gene="msrA"
                     /locus_tag="ACG81C_000934"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5064114.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13995..14672)
                     /gene="pgmB"
                     /locus_tag="ACG81C_000935"
     CDS             complement(13995..14672)
                     /gene="pgmB"
                     /locus_tag="ACG81C_000935"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5064115.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(14669..15797)
                     /locus_tag="ACG81C_000936"
                     /pseudo
     CDS             complement(14669..15797)
                     /locus_tag="ACG81C_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(15797..17980)
                     /locus_tag="ACG81C_000937"
     CDS             complement(15797..17980)
                     /locus_tag="ACG81C_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5064116.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17977..19002)
                     /locus_tag="ACG81C_000938"
     CDS             complement(17977..19002)
                     /locus_tag="ACG81C_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5064117.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19047..19874)
                     /locus_tag="ACG81C_000939"
     CDS             complement(19047..19874)
                     /locus_tag="ACG81C_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5064118.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19871..20938)
                     /locus_tag="ACG81C_000940"
     CDS             complement(19871..20938)
                     /locus_tag="ACG81C_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5064119.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20964..21800)
                     /locus_tag="ACG81C_000941"
     CDS             complement(20964..21800)
                     /locus_tag="ACG81C_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5064120.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21797..22666)
                     /locus_tag="ACG81C_000942"
     CDS             complement(21797..22666)
                     /locus_tag="ACG81C_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5064121.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(22736..24019)
                     /locus_tag="ACG81C_000943"
     CDS             complement(22736..24019)
                     /locus_tag="ACG81C_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5064122.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24033..25718)
                     /locus_tag="ACG81C_000944"
     CDS             complement(24033..25718)
                     /locus_tag="ACG81C_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5064123.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25993..27018
                     /locus_tag="ACG81C_000945"
     CDS             25993..27018
                     /locus_tag="ACG81C_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5064124.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         8005 a         5725 c         4878 g         8949 t
ORIGIN      
        1 gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt cttcgccaac
       61 acaatttgac gaagagcctt ttctttcagg aagacttcgg taatttttag gagttctacc
      121 tattctttta accgtttatt ctctttttct aagcgtgtaa tttcatttct gcgaatggat
      181 ttcatgactt cctctaccga gaaaagtacg ttatccatat ttttcaactt cttgaactca
      241 tcaatacacg ctattagagc gagcttctag ctatagacta acttctttaa cagaaaaccc
      301 ttcttccgtt accagtttta cagcggtaat tttaaatatt tcatcaaatg aacgtcttgg
      361 cataatccag atcttcgctt tctcttaatt tggtgtcaat tttagtataa cccgtccaac
      421 ttatttttta actaagtgcc cggtttttct tgacaactcc acccaaaagt tagatttttt
      481 ggtctaagtt ttagggtcgc ttcattacta gaattcaaca agagatgttt tttattttat
      541 ttaaattatg gggtcagttt ccatttgatt acaaacttct ttgttttctt tttttaaacg
      601 caataataaa ggttttaatt tcagcctttt taattagaaa atcaattttc ccaaattatt
      661 ttctttagct ttcttaacga tttctgtcgc aactaccaca tctaatacag ccgaaccaac
      721 tgttttgaag atcgtgattt gttctccact tgtccgaccg atcaattctt cattgatcag
      781 ttggcctagt tccccatgat agctgtctct ttgaatatac ccatcttgca gcggtgaaat
      841 gaagtcccca gcttctttta gaacaccgtc catggtatca aaaataacaa catctgcggc
      901 tttgattatt tctcgtggaa tttcacacat ttccggtgtg taagcaccga ctccattgat
      961 gtgcgcacct tttttgaccc actcagcaga gaatgtcgca cgttttgaag tggtcacact
     1021 tgtaatgatg tctgctcctt caacgcattc ttgactggtt tttgttgggc gcatcgttac
     1081 agaaaattgt tgcatcattt tttcagcaaa ttggctggca cgctcaaaat caatatcaaa
     1141 aatccgaact tcttctaatt tacgaacggt caacattgct tctaattgtg actgtgcttg
     1201 tccgccggtt ccaatcagcg cgccaatttt agcatcttct cttgcaagaa gttctgttgc
     1261 tgctccttga acagcgcctg ttcttaattg tgtcaaataa gtaccatcca aacaagcgga
     1321 taccataccc gtttctggat ccaatacgat catcgttgct ggtacacttg gtaagttttt
     1381 cttgatgttt tcaggataca cggaaacgat tttgacacct agcgcgcctt cgccaccttc
     1441 tacataagca ggcatatata agctttgtcc gttgcttttt ggcacatcga tattggtgcg
     1501 aagaggcaca cttgcctttc ctgctgaata caatgacagt gcttttttgt ccgcatcgat
     1561 ggcttctctc attgaaaaac ttttgataat atcttctttt ttaagaaata acatttgaat
     1621 cgctccttaa ttagctgctg taactttttt taattgactg cccaacatcg tatatgcact
     1681 gtatagtaag accaaaatca cgatccattt caggatatag gtatctaaag atttgactag
     1741 gaatacagcg accaataccc ccaatacacc gaaggttgaa gtaaaaagtg tgatttttct
     1801 gctgtattca ttaaatttaa tgaattgaac actgccgatc ggtactgaga aggtacaagc
     1861 ccccatcata ataggaaagg ctgcaactgg attcaagcct agcaagtaga cagttaccat
     1921 cgttaacgca taagaaccaa tgccgatatt gttcaatgca ccatagacaa agagtaaaaa
     1981 tcctgccaag attaattttc cgccatacaa ttcactggct gttccgtttg atggaatcca
     2041 gttcatttgt cctgcaaaaa tcaacaatgc agcaatgacc aatcctatgc cgacaaacag
     2101 tttgatcgtt ttctctggta acttcacgac aaatcttggt cctaaatatg caccgataat
     2161 ttggcagata atacaaacag ctagtgtctt gatccctacg ttgatggcag tgatgtaaga
     2221 caatgccatg acggctaccg gtactacaca ttgggtattc aacgttccgg gtagtttttt
     2281 catgctgacc catttcaact ttggataaag gacggttccg atagcaaagt cggaaacgcc
     2341 aaatgttgaa agaaagaaga tgataaatga cgtgaagggc aacagacgca tatcccctgg
     2401 ttcttctttg aaattttgtt tgtttttgaa aaggtctctt agaaagtaaa taataaaata
     2461 tagattgatt gcgacaatta gtgtcagcaa aatattccga atcataaaaa attcctccta
     2521 ctagtaaatc tttctgattc cctcaaagcc ctcaccatat tttgctttaa gttcttcagg
     2581 agattctgca atcactttta gtgtatatgc atcatgtttg acatattctt ttgctagatt
     2641 gaccatggcg tggttttttg tccaagtgac atcggttgat tgcagttctt cgttaatggt
     2701 accaaacacg acttcttgct catcggcgac gatgttgatc catcttgctc caaagtcagc
     2761 aagtttgtct gtttcaaaac catgaatata atacttttct aagggaagat catattcatg
     2821 ggtagaactg aataaaatca gtacaaactt atcaactctt ttttcagctt gctgcaatgc
     2881 ctttagtagt gaatccgtga aattctcatg ccaaacttga atgagcaaac tttcttttgc
     2941 gtttttcacc aaatgctcta ctttatccag aacatactcg ataccatcaa ctttccacag
     3001 catttcttct tcatcttttt ctttgatcat ccctagattc ttggtaagaa aagaaagatc
     3061 atttttaaca cttttttcta atttctctaa aaactcattt gccgagatgg catgataaag
     3121 tttaggttct gatttattaa cgacaatcaa attctttttt aatagttttt ccaaaatatt
     3181 atatactttc gagcgtggca cacctgagat tttacttact tcatatcctg taagattacc
     3241 tttttctaat agtgtggtat acactcttgt ctccatctct gaaaaatcat attttttcat
     3301 gatttgaata atcgtttcca ttgattaatc ctcactttat ttttagtagc tactacggta
     3361 acaactataa attctttttg attatctgtc aagagttagt tcaataacac acatataata
     3421 attagcttaa aaacaatctt tttattaaaa agaaacatta taatatgaat gtttgtgaaa
     3481 aaataataca acttagtcat tctttacaac gatttataga aaaacaacct ctttaaatat
     3541 aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat tctttctaaa
     3601 aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta tggtcttgtc
     3661 cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta ggttttggac
     3721 aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga tatcatcaca
     3781 ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag atttcactgt
     3841 gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta atctaagcga
     3901 tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg gaaaaacttt
     3961 tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc ctaccacacg
     4021 ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct ccaaaaacaa
     4081 aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc gtccgcctcg
     4141 gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat aagataaatc
     4201 atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg taaaaactta
     4261 tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt acccacctgc
     4321 acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc attataatga
     4381 ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa ggcaccaatg
     4441 aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac cctttccttg
     4501 caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta tagcggcaac
     4561 cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc gcaaacccgt
     4621 agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc agtccctacc
     4681 aaacctgtac tattttttta caggaggttg ctatgttcaa aatctttcgt aaaaattgtt
     4741 gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca gatgcgaatg
     4801 gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc aatgaattag
     4861 ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc ggtaaatttt
     4921 ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct catccgaagt
     4981 acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg gatttgtgat
     5041 ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat ccgccagctt
     5101 cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga aaagaatcgt
     5161 gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag tgatgtgttc
     5221 ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga atcatttgat
     5281 gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca agcggcagtt
     5341 gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa ccatattaac
     5401 gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc agaacccttc
     5461 tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc aataacagcg
     5521 attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc gaaacacttt
     5581 atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac aaaatcaaca
     5641 cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc caatgctctt
     5701 ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa agcgaatcgt
     5761 ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat ctggaatgtc
     5821 ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc tgcgaaagaa
     5881 gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg atataccatt
     5941 atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg ttgtcttcca
     6001 tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg tatcttcaaa
     6061 tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct tagcatttaa
     6121 gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc tcctagtata
     6181 taggcagact gccaattata actgattcaa tccacattat gataaaatcc accttttcgt
     6241 ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca ttcagtgctt
     6301 tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt acaaaatcag
     6361 aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg caacagattg
     6421 gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg ctgtttccca
     6481 gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag caattgacta
     6541 cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat caaatcaaaa
     6601 atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg actgtatcat
     6661 aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat tggacagttt
     6721 tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct cgactcttgt
     6781 ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa aatagaaaag
     6841 agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct ttgtggatcc
     6901 attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg tatttgataa
     6961 tttatcttaa caaccagata attttattca gcaccgtatt atctatattg aattctcgga
     7021 atcaagataa atttttttcc gtttttgttt cttttttaat cttcttcttg ttattaatag
     7081 tgattaggat gatattccta tattgatctt tgataattag atataatttt tgaataacgt
     7141 tccctgctat tcgagctaga aatacaagta cgccatgacc tagttagcga caactactta
     7201 gagatttctt ttgttctggg acaacaaaat gccatgacaa tagcagtaaa taatgatact
     7261 tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta cccaaagcgg
     7321 ttcaaaaatt ttaaactatt attttaggag atgattttat aaaatcgaaa gcctacttga
     7381 ataaaaaaca agcctgtgaa tttctaggaa ttgataataa tactttagat aagtggattc
     7441 gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac gtagaggata
     7501 tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc acatatacta
     7561 taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc ctacaaagct
     7621 ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag aaatcactgg
     7681 aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta ttaaggccga
     7741 aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa actcactaga
     7801 attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc ctagatatga
     7861 actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta gtaatactaa
     7921 ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga attatctgaa
     7981 ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat acgcaaatat
     8041 aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac tatgttatat
     8101 cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga aaatggcttt
     8161 agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc tgactgattg
     8221 gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg attacacact
     8281 tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc tgcaatggaa
     8341 atatattgat tttaataaaa atacggttcg tctcaaatat gctttagata aatatcaaag
     8401 aaagacacat acaaaaggtt ggaaagacac cgttattcag gcaccagaag ttgtgatgac
     8461 attattaaga gaatggaaat cagctcaggc tgaacaattg ttgaaactaa aaattataca
     8521 aacacctgac cagtacctct ttacctattc caaaccttct ggagaagtaa attgtcctgt
     8581 tcacgctgac tacttaaact atcgaatcaa tgcaataaaa aaaaggcatc ctgaattggc
     8641 tcaattatct ccacacaaac ttcgtcatac ctatgcaact atagctaggg aaggtggagc
     8701 aaatatgaat caaatctcta atgctttaac gcactcggat atttctacta caaaaatata
     8761 cgtgaacaca cctgacgtag ttgataaatc tgtttttgaa gcatttcaaa aaggattgaa
     8821 taagtgtgat taacatgtgt tctcaaatgt gttcttatta taaaaaaaga cactttccaa
     8881 ataattgaaa agtgtcctga aatattgata taaaaagaat attaacgttt tgagaattgt
     8941 gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg tgcgtcacga
     9001 gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca
     9061 cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta
     9121 acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca
     9181 cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca
     9241 gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat
     9301 tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct
     9361 ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt
     9421 ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga
     9481 gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg
     9541 tgacggtaat aaattttgtc agtcgctttt ttacctgcta atttcacttt gtctgcattg
     9601 atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca
     9661 cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact
     9721 acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt
     9781 tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg
     9841 gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct
     9901 tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa aagggtcgtt
     9961 tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca accccttatt
    10021 ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccataaaaca
    10081 taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca ggcttacaac
    10141 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
    10201 atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
    10261 cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc gctttgccca
    10321 aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg ataaagatat
    10381 cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg taaagatctg
    10441 ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta cgatcccagc
    10501 ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta gaataaggtg
    10561 aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc aaatcatcca
    10621 tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg taccaatgtg
    10681 tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt aatacttctt
    10741 tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt aaaataatag
    10801 tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt cagttcgctt
    10861 ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca gttgagcaaa
    10921 ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat agattttctg
    10981 atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta tcaattcctg
    11041 tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca cagctcctaa
    11101 tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat cagccatcat
    11161 ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg tgagctgatt
    11221 ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt ctagaatcgc
    11281 cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc caaacgcatc
    11341 tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca gaccattggc
    11401 acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt ctcctttttc
    11461 tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa agatgccagc
    11521 accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat aacaaaacgt
    11581 ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc cgctcgttcc
    11641 tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag aaagcggccc
    11701 gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg ctgctgtttt
    11761 tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt gctctattcg
    11821 aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat caaaatatcc
    11881 agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat aaaagatata
    11941 gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc tgttttgcag
    12001 ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct gccgataaaa
    12061 ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc ggttatctgc
    12121 ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca ggccatgcat
    12181 cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt taattacttc
    12241 ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg ccatcccttg
    12301 tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg cttcatctgt
    12361 gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa cgttcatttt
    12421 ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat cttggttttg
    12481 aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg gatcattttt
    12541 atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta gatctcattg
    12601 tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct cgatcatcgt
    12661 ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt tgttggcaga
    12721 agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa gaaatttttc
    12781 gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat tttacttcat
    12841 ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt ggttgcaggc
    12901 cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg tgtgccttcg
    12961 ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt ggagtggtgg
    13021 cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg gaatatccgt
    13081 tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt tcattccttt
    13141 tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg ttgtgacaaa
    13201 cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg acttggagct
    13261 aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc ctcagtatta
    13321 gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag ctttttcggc
    13381 ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag aaccttctcg
    13441 ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat cttctgcagg
    13501 ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc gcccgctgtc
    13561 ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat tatagtagat
    13621 cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag tcggatcggt
    13681 ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat catattcgat
    13741 ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag ttggatttgg
    13801 tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct cttcaaatgg
    13861 tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca tataaaatcc
    13921 cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc ttgtgacaga
    13981 aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa cgaacgtact
    14041 tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt tatgttttca
    14101 cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc ttctgaatct
    14161 tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt gagaaaaact
    14221 tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc aaaataactt
    14281 gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc caatccgatc
    14341 ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa aacggatgtt
    14401 tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc aaaggctgat
    14461 ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg ctctagtgac
    14521 tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg aatggacagc
    14581 tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac aagaacgcca
    14641 tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa tgaacgactt
    14701 gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct tgctgtaaag
    14761 cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg ccaacatctg
    14821 ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga acgccctctg
    14881 gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca tctagacgtc
    14941 cttcgcctac gattacgagg tctgcttgct gtacccgtat cgaactccag taaatccaat
    15001 acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc tgcacccatt
    15061 ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct catataaaaa
    15121 tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt ttgtggtccg
    15181 aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt aatgacagtg
    15241 attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc aattttacct
    15301 agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc ataacctaga
    15361 ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc gatgatgatg
    15421 tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg cgtggataac
    15481 ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc tgccatttca
    15541 aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc aaatgcatta
    15601 gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga aaccgttcct
    15661 tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac ttcttgaaaa
    15721 cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa agaatctggc
    15781 gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt tccagctggt
    15841 attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt attactattt
    15901 gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga aaagaaattt
    15961 tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt actgtaatac
    16021 ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca tgaattccgt
    16081 catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg aataaatgat
    16141 acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct ttactcaacg
    16201 atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg attggttgag
    16261 aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg ctgacttgga
    16321 gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca ggacgttctt
    16381 tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc ggaatcagct
    16441 gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat tctttcaaaa
    16501 aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc gccagatagt
    16561 tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg actctgtgta
    16621 tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct gtttcatgaa
    16681 tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg acatactctt
    16741 gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa gcaacttctt
    16801 gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt
    16861 gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct ttttgccgtg
    16921 cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca ggaaactgat
    16981 ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct ttataacctt
    17041 cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga gtcataatct
    17101 gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg ccttggatcg
    17161 ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct aaaatttctg
    17221 tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct gtttgctgaa
    17281 gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt ctgatctttt
    17341 ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct ttattgaatt
    17401 tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca ctgatcaaat
    17461 cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct
    17521 gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc ttgatgacca
    17581 ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta atgacttcca
    17641 ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc atttctaaat
    17701 ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt tcaccgtcga
    17761 tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat tctgctccta
    17821 aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga tagtcttcct
    17881 cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc gcatacttat
    17941 tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa cttctcctgt
    18001 ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt gccagccatc
    18061 tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca tacttagttc
    18121 atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat ctacatcttc
    18181 gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag ccacattcag
    18241 tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct tgccgttttc
    18301 taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat cccatttccc
    18361 gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc gaacgctttt
    18421 tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc cataatcgat
    18481 caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg gaacaccaga
    18541 ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa gatgattctt
    18601 gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt
    18661 tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg gtggtttctc
    18721 gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt gaaagcgatt
    18781 ggctgtacag acactcacga tatcgggaga aatctcattc agcatttgtg taactgatgt
    18841 atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc tgactggatc
    18901 aaccacagcg gccaattcta agacatctga tcttttttga taataagcag catgtacttt
    18961 ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct ctctcctttg
    19021 cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa atgacgcgct
    19081 gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac ttcattttct
    19141 cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag tagcgtttga
    19201 aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt atctgctaaa
    19261 tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc atcttcttca
    19321 atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt agagtcaatg
    19381 taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt caaaggctcc
    19441 aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag tgattcgctg
    19501 actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg
    19561 aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct tagtgcacgc
    19621 aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa gtcaccgatc
    19681 cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt gattgcctgt
    19741 ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc ttcaaatcca
    19801 cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc
    19861 gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata ctttcttctg
    19921 ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc acaactggat
    19981 caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga
    20041 ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct cgagcaaaga
    20101 cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt
    20161 tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa gaagcttgta
    20221 atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta tctgtcaatt
    20281 cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg gcaccgtatt
    20341 tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta gtagcacctg
    20401 cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag attgctacag
    20461 catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga gcaggatcgt
    20521 aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta taattatcaa
    20581 caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac tctccttttt
    20641 gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata tttcctaatt
    20701 gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag aataggcgcc
    20761 attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg acaatttctg
    20821 ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt tcgatctcac
    20881 ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat tttttcatct
    20941 tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa atcaccttta
    21001 ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac caatgaagcg
    21061 gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa accgattggt
    21121 aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc attccacgaa
    21181 accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg tataatgatc
    21241 cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc aatatcagtc
    21301 ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt ttgaacgacc
    21361 atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat gattttaaat
    21421 aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct agcattgatt
    21481 ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat cccaatcact
    21541 actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata catgatgaat
    21601 ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc tttcggaaaa
    21661 atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat cagcatgatc
    21721 aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa taagattttc
    21781 ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag acgaccgctg
    21841 caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca ttgaacgcaa
    21901 aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt agatagacat
    21961 ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct agtactggtt
    22021 tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg tccatttcag
    22081 ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat
    22141 acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca actggctggt
    22201 cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga tcattgacga
    22261 ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata gatggcgtaa
    22321 cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg cggttaagca
    22381 aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat gtgtaagcaa
    22441 ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga taattgctta
    22501 atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa
    22561 tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt ggtgctaaaa
    22621 gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa acctccccct
    22681 gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact tcttgttatt
    22741 tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg acagttactt
    22801 ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc gtgaaattct
    22861 tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc tcagatggca
    22921 gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg actacttgtt
    22981 tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg tttttcggtt
    23041 gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct agaccagaag
    23101 aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt gggatagcaa
    23161 aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa gttgaataga
    23221 ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca tcattagagc
    23281 ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc atcttttccg
    23341 tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac aaagcaaatt
    23401 gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt ccatattgtt
    23461 tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa ttttctggtt
    23521 ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga acccagccgc
    23581 taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt ttggctccgt
    23641 cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc tgatcaatca
    23701 gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact tctggtaatt
    23761 taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct accggcactt
    23821 gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca ttgatcacac
    23881 ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt
    23941 tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc agacaggtag
    24001 taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag ccacatatat
    24061 tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg tccagaaaac
    24121 aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga aaaattatgg
    24181 ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga atctgctgag
    24241 tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg aatagatagc
    24301 atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa ctctttctca
    24361 atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt tttttctacg
    24421 cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg cacgcctggt
    24481 aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc cgtcgtatct
    24541 gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact ggtcgttcca
    24601 tctgggtttt tcttgtagtt tactcgggca ccctctttct tcagctgttc cactaattca
    24661 aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc gtcatgggag
    24721 gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt taaccattct
    24781 ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac
    24841 tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt atctttgtgt
    24901 ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc gattgcacgg
    24961 aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc tagttctttc
    25021 cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa
    25081 agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc tacttgatct
    25141 tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt
    25201 aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg caaatcaata
    25261 aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt actttcggca
    25321 gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat ttcttctata
    25381 tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga gaaaccatca
    25441 tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc tgccaaatac
    25501 ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc
    25561 ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc ctggcctttc
    25621 ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc agacaaatct
    25681 gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt tcttttttgg
    25741 gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata gcattttttt
    25801 aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa taattaatag
    25861 cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca
    25921 tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc caaaaaggag
    25981 gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa tgtctcaaaa
    26041 tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc aaaagcaagt
    26101 gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag aggactgtca
    26161 ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac gacctatggc
    26221 aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca aaataacttc
    26281 gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag
    26341 atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga tgaaacactt
    26401 ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt cggaaacgac
    26461 tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga tttggtggaa
    26521 acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac agaagtttat
    26581 gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca cctagatatc
    26641 cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc agcaatgaat
    26701 gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc attaaaatca
    26761 ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga tctttcgatc
    26821 gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac tcggatagac
    26881 attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact aatccaccac
    26941 caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac agattcgatc
    27001 caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg ataagtcagt
    27061 tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat tttagcgaat
    27121 ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt gtgttaaact
    27181 attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag tttttatagt
    27241 tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga tacttatctc
    27301 taaaacataa atagaataac agacatgtct actggttttg ttttattcga ggaattattc
    27361 aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat ttattttgca
    27421 ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga tgatcgttcc
    27481 gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt tcttctagtt
    27541 agacaaatta ttttaga
//
LOCUS       DAZUMI010000025        27212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000025 DAZUMI010000000
VERSION     DAZUMI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.025"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..444)
                     /locus_tag="ACG81C_000946"
     CDS             complement(160..444)
                     /locus_tag="ACG81C_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064125.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="ACG81C_000947"
     CDS             complement(462..1352)
                     /locus_tag="ACG81C_000947"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5064126.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="ACG81C_000948"
     CDS             complement(1372..1590)
                     /locus_tag="ACG81C_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5064127.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3240)
                     /locus_tag="ACG81C_000949"
                     /pseudo
     CDS             complement(1560..3240)
                     /locus_tag="ACG81C_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            3507..4280
                     /locus_tag="ACG81C_000950"
     CDS             3507..4280
                     /locus_tag="ACG81C_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064128.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4361..4930)
                     /gene="thiT"
                     /locus_tag="ACG81C_000951"
     CDS             complement(4361..4930)
                     /gene="thiT"
                     /locus_tag="ACG81C_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5064129.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(4996..5098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5104..6075)
                     /locus_tag="ACG81C_000952"
     CDS             complement(5104..6075)
                     /locus_tag="ACG81C_000952"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5064130.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6169..7542)
                     /gene="glmU"
                     /locus_tag="ACG81C_000953"
     CDS             complement(6169..7542)
                     /gene="glmU"
                     /locus_tag="ACG81C_000953"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5064131.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8045..8863)
                     /gene="purR"
                     /locus_tag="ACG81C_000954"
     CDS             complement(8045..8863)
                     /gene="purR"
                     /locus_tag="ACG81C_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5064132.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9030..9854)
                     /locus_tag="ACG81C_000955"
     CDS             complement(9030..9854)
                     /locus_tag="ACG81C_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5064133.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9827..10507)
                     /locus_tag="ACG81C_000956"
     CDS             complement(9827..10507)
                     /locus_tag="ACG81C_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5064134.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10530..11468)
                     /locus_tag="ACG81C_000957"
     CDS             complement(10530..11468)
                     /locus_tag="ACG81C_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5064135.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11713..12564)
                     /gene="ispE"
                     /locus_tag="ACG81C_000958"
     CDS             complement(11713..12564)
                     /gene="ispE"
                     /locus_tag="ACG81C_000958"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5064136.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12718..15291)
                     /gene="mprF"
                     /locus_tag="ACG81C_000959"
     CDS             complement(12718..15291)
                     /gene="mprF"
                     /locus_tag="ACG81C_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5064137.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15432..16238
                     /gene="proC"
                     /locus_tag="ACG81C_000960"
     CDS             15432..16238
                     /gene="proC"
                     /locus_tag="ACG81C_000960"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5064138.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16317..17873)
                     /gene="rny"
                     /locus_tag="ACG81C_000961"
     CDS             complement(16317..17873)
                     /gene="rny"
                     /locus_tag="ACG81C_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5064139.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18284..19333)
                     /gene="recA"
                     /locus_tag="ACG81C_000962"
     CDS             complement(18284..19333)
                     /gene="recA"
                     /locus_tag="ACG81C_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5064140.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19442..20683)
                     /locus_tag="ACG81C_000963"
     CDS             complement(19442..20683)
                     /locus_tag="ACG81C_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5064141.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20923..21501)
                     /gene="pgsA"
                     /locus_tag="ACG81C_000964"
     CDS             complement(20923..21501)
                     /gene="pgsA"
                     /locus_tag="ACG81C_000964"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5064142.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21710..22633)
                     /locus_tag="ACG81C_000965"
     CDS             complement(21710..22633)
                     /locus_tag="ACG81C_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5064143.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23371..24657)
                     /locus_tag="ACG81C_000966"
     CDS             complement(23371..24657)
                     /locus_tag="ACG81C_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5064144.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24650..25918)
                     /locus_tag="ACG81C_000967"
     CDS             complement(24650..25918)
                     /locus_tag="ACG81C_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5064145.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26170..26631
                     /gene="mscL"
                     /locus_tag="ACG81C_000968"
     CDS             26170..26631
                     /gene="mscL"
                     /locus_tag="ACG81C_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5064146.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26690..27136)
                     /locus_tag="ACG81C_000969"
     CDS             complement(26690..27136)
                     /locus_tag="ACG81C_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064147.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7735 a         5868 c         4722 g         8887 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct tttttcatat ctccttgtaa
     2701 aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg ataatctcaa agttgatatc
     2761 atgttccttc agcgcctgcc acacaccaat ctgataagca ccgtgcgctc ctcctccacc
     2821 taaaacaagt gctgtatgat agaccagctt tttatttaat tgtgtggttc cttgatagcc
     2881 gtttttatga aaaatacgca accattcttc tgatagagga gattgaaata aaaaacaaca
     2941 gttttcttta ataaaaaaac gagcgcaagc ttccatatac cgaaaaaatt ttttccattc
     3001 aaaagtttca ccttcaatca acagattatt aaaaataaca tctcctctac ttttagcaat
     3061 agtaaaatag acggtatatt ttttcgatac tgctttaaaa gcacattgct tttttgcaga
     3121 acgccaaaaa gcgtctctac tttcttgatg actttgaaaa aatggaaggg gttgttttgc
     3181 ttgctggata ggcgaataca gcgacatttt ctgtgaaaag taaacttttt ttatctccat
     3241 cgcgctctcc tttttcttca agtataccag tacgtcataa aaaggtcatg agtctcgtat
     3301 tcgactttgt agattccata agaaaacaat tcataaaacc tcctttaaat aaaaaaatac
     3361 aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa tgaaaacgtg attttgtaat
     3421 ggaattttaa aataaaagaa acaagctttt ttaatgcaaa tgttgtaaaa taaaagagat
     3481 taagaggaaa taagaggagg aacatgatga ctgagtcagt ttatgtacat attgatacca
     3541 caagtaatgc tgttttaaca aaagggctga ctgctactga ctttgcaaac agtatcgtgc
     3601 attttcctca aaacttgctc ttgcttgatc catctgcttc tgctggagaa tatgaatccc
     3661 atacagggct gaaagtaatc cgtggtacag aaaatattca gcgtttcttt tcctcttctc
     3721 gtaacaggcg tggattcgat gaattgaagt ggatcgactt tactgattta acgatgctga
     3781 aagaattgac acctttagaa atttcagaat tattatattt tggtcatatg aaaacacatc
     3841 ttcattcgcc gtttttttat aaactacaga ataattttgt atacttcgat cttaatgatc
     3901 agctgaatcg gatttattat cgctatttag atgaatttta taggattttt gctagtaaat
     3961 taagttcgat cttgtcagat aagatcaatg taaaaaaatc ttttttcaaa aaagaaacac
     4021 ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg taatttgatg caggaaggga
     4081 tcattttttc ttttgatcaa gttggtttga tcgatggtaa atatcgtatc ccaatctatg
     4141 tgattgaaga ttatatttgg aaattaaaag gcacccggta tcgagaagag gaaatcatcg
     4201 ctactttaat ctactctgtc accgatcgac actggcaatt ggtagaagat gccgatcttt
     4261 cttatctgcc attgaagtag atctatttct tatcaaaaaa taaaaaggaa acaagaaact
     4321 aggacatgat tctgtctcaa gtttcttgct tcttttttat ttacagctcc tgtcgtttat
     4381 ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa taaaacgatg atcgttacaa
     4441 tagctgtact caacccgctg gcaccattca ttacgaaaga aaagagccaa ggattcatcc
     4501 cccatagtgc atacgctccc cagaaaacca ctcctgcaat aaaatgccaa aaatatctgg
     4561 aaagtgcgcc aatcaatgca ccaaacacga tgctttttct aatttgtttc gtattatcgt
     4621 ctacatagcc tttttggaca tttgaagcca tgagccctgc tgcgcctgca aaagtatagg
     4681 caatcgggta ctcaatcaac acttgaacga cacttaaata ataaacttgt cccaacggaa
     4741 aatgtaggat gccccaaatg aaacctgcca gcatccctgc ctttgtccct cttcttaacg
     4801 caaaaagagt caatggaatc tgccctaatg aaatagaaaa actgctgccg atctggatcg
     4861 gaatcaacga caacaccatc gctaacgctg caacaatcgt tccttctacc caaactctat
     4921 tttttcccat aaaaaaactt cctcctttga ataaaaaagt cacaaaggag gaagcagctt
     4981 tcgaaaatag tcaaacagct gtttattcac aattcctacg ctcgcataac cgatacaggt
     5041 acgaagggtt tagaaataga ttcaatctca gcccaattgg actccccttt gtgagtttat
     5101 ttattattta ttttttgttt caaacaatgg actaactggt ttgttttcat ggatacgttt
     5161 gattgcatct cccattagtc cgccaacact tacttcatcg attttgtcga ttttacgatc
     5221 ttctggaaga tagatcgagt ctgtaacaac tagacgctcg atagctgaat cttctattct
     5281 ttgtaaagca gggccggata atactggatg tgtacatgaa gcatacacac tagttgcacc
     5341 agcttctttc aaagcattgg cagccaatgt gatcgtacct gctgtatcga tcatatcatc
     5401 gatcagtaca caagttttgc ctttgacttg tccaatgata ttcattactt ctgcaacatt
     5461 tgcttttgga cggcgtttgt ctatgatcgc aatcggagat ttcaaaaatt ccgctaattt
     5521 acgtgcccgt gtgacaccac catggtctgg tgaaacaacg acaacatcat cgcctttgat
     5581 tcctttttct aagaaataat tggcaataag tggcgcaccc atcaaatgat cgactggaat
     5641 atcaaagaaa ccttggattt gaactgcatg aaggtccaaa gtcagcattc ttgttgcacc
     5701 agctttttga atcatgtttg ctactaattt cgctgtgatt ggttcccgtg cacgtgcttt
     5761 acggtcttga cgcgcatagc cataataagg catgactacg ttgattgttt ttgcacttgc
     5821 tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag ttatcattga ctgggctgct
     5881 cgttgattga ataacataga catgcgatcc acgaatactt tcttcaatgt ttacttgaat
     5941 ttctccatca ctaaattgag taacagatga ttttcctaac tcgacgccga ctgctgctgc
     6001 aattttctct gctaatgggc gattggaatt taacgcaaaa atttttaatc ttgggtcaaa
     6061 gtaatgtttt gacatgggga cctccacttt ctttttcaca actctagaat tctacctaat
     6121 aaatagtagt catatttttt aaataaatca agtgattttg caaaagtctt agtttagata
     6181 agggagtttt tttgcatatc cttctttatt gacttgtctt gctctcgcaa ttgccagcgc
     6241 atattctgga atattgtctg taattgttga cccggcagca atcgaactgt ttttagctac
     6301 ctcgacaggt ccgatgatat tcgtgctaga accaataaag ctgtggtcgc cgacagtcgt
     6361 atgatgtttg ttctttccat catagttgac gaagacaaca ccacatccga cattgatttc
     6421 ttctcccagc gtagcatcac caacataagt caagtgaccg actttagttc ctttgccgat
     6481 ttgagcattt ttcacttcca caaagtttcc gatatggacg ttttcgccaa tttcagcttt
     6541 tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc ttgacatggc tgctttcaat
     6601 gactgagttt tcaatgacga cgtgatcctc aatcctactg tctacgattt ttgaatgtga
     6661 cccgatgaca caatcagaac caatgactgt atttccttgg atttgtacac ctgcttcgat
     6721 caatgtgtct ggaccgattt ctacgcctgc atcgatgtat gtcgttgctg aatcgacaaa
     6781 gcttacgcca ttgaccatat gcatctggtt aattcgcttt ctcatgatct catttgcttt
     6841 agctaacgcg atccgatcat tgactcccat tgattcttca aaatcatccg tttgataagc
     6901 agcaactgta tggccttctt cttttaagat ttcgatgata tctgttagat agtattcacc
     6961 ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac aaagcttcgt tgtcaaaaca
     7021 gtaagttcct gtattgattt cttgtacaag agcttcttct ggtgttgcat ctttttgttc
     7081 cacgattttt tctacgatcc caatatggtc acggatgatt cgtccatatc ctgttgggtt
     7141 ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt ccttggtgat attcaaaaag
     7201 attgttcagc gtttcagtag tcaataatgg tgtatcgcca ctgataacta acgttgttcc
     7261 tttttttcct tccaagaaag aagcagcttg caaaacagca tgtcctgttc ccaattgttc
     7321 tgcttgtaac gcatatttac tgcgttctcc tagttgtgct ttaacttgtt ctgctccgtg
     7381 tccaacgatc gtaatcactt catctggttt cgtttcactg acccgattaa tgatatgttc
     7441 taccataggt ttaccagaaa cgggatgtaa aactttatat aattttgact tcatgcgggt
     7501 acctttccct gccgccaaaa taattgcata acgtgcgtcc aactgcgtca ctcctaaata
     7561 tagtttgctg atttcagttt atcccagtat acccattttt tcaacaaaac aagtgatttt
     7621 cttttatccc agcgttttct ggataaaaaa atagatagac cataacgggc ctatctattt
     7681 ggtaaccggt gctctcctgc caactcctcg gtcacaagtc acactactca taaaacatga
     7741 aaagctgttt tttgaaatcg tttcttgtga cttagagtta aacggtaggc ttacaacctc
     7801 ttcattaaac gtttttcaat cagctgacct tcggtattag ctcaataaac ggcaaaatat
     7861 gaggagcaat ttttccgttt attgagctaa tactcaggac aggacgctga tttcacaacc
     7921 tctagataaa cggtgttcaa aagccagttt ctcggtatta agccaaattt atgaaaaaat
     7981 gtggaaagcc attttcacaa aatttttctt actactcgaa actaagcagc tttttcacaa
     8041 cctcttattc tgaaaaataa tttcctggca caaccgtaat atttttcgtt tgagtatcta
     8101 catctttgac ataaagcagt gacgtataat catcgatggc gcgatgaccg ttgaattttg
     8161 attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa ttcttcaatc aagctcttca
     8221 ttccgttgac tgttccgccg cctttcatga aatcatccac tacaaggaca cgtgaaccac
     8281 gtttcaaact tctcttagac agttccatct tttcaattct ttcagaagat ccagacacat
     8341 agttgacact tactgttgat ccttctgtta tttttgagtc ccggcgtaca ataacgaatg
     8401 gcgcattcag ataataggac acagcttggg caataggtac gccttttgtg gctacagtca
     8461 ttacagcatc gattttttca ccaaggtatt tagaagcaat gattcttcca acttgacgca
     8521 gcagttcagg ttctcctaac aaatcagaga gataaacata tcctcctgga agcagacggt
     8581 cttgttctga tagacgttct gctagagatt caatatattt tttcgcctct tcatatgaaa
     8641 tttcagggat gaacaataca ccacctgcag cacccgggat tgtttccaag gttccatacc
     8701 ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc actgatagaa gatttagcag
     8761 attcataacg ttcggcaaag cttgttaagg aaatcaattc atgcgggtga ttcaataaat
     8821 actgtgtcat gtcaactaga cgttcactgc gacgtacttt cacctattcc aactcctttt
     8881 aagaaatttt atcgttttac attatataag tttttcttca aaaattcgag cattttatga
     8941 aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa aaaatttttc
     9001 gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa ggcaaccaat
     9061 attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt gattgttgct
     9121 cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc tagaaatcct
     9181 acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat accgattgct
     9241 gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat catcacagca
     9301 atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg tagcccatcc
     9361 acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa taaaaagaac
     9421 aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca tgtaatggtc
     9481 acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc gctcaaattc
     9541 atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga gatttctgaa
     9601 taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac aacgactagc
     9661 atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc tgctaaagag
     9721 acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa cacacctagc
     9781 atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat gaattcatat
     9841 gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg tccgcatact
     9901 gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg acatgcgcac
     9961 tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct gttgttggtt
    10021 catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg attcgctgct
    10081 tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc cctacagctt
    10141 ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac cagcggcctc
    10201 ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct gcattgaaag
    10261 atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga tttttctcag
    10321 caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc accaaggttg
    10381 acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct acatgataag
    10441 agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat atttcgatat
    10501 aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa agctttcaga
    10561 tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt agtcaagctt
    10621 tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt ctcagctacc
    10681 ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt cacaaattct
    10741 tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc cgaaatcgct
    10801 acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg agtgacaaat
    10861 gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc ttcaagcttt
    10921 ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata tttttcgcct
    10981 aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag ccaaacatgt
    11041 ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc gtctgcttct
    11101 tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg tttgctatcc
    11161 actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata cacgaaaaca
    11221 tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg cggctccgtt
    11281 ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt tgtaaaatca
    11341 tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc ttccttgttc
    11401 gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt caaaagatat
    11461 tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt attaacgtga
    11521 actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc tgatttaagc
    11581 atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct agccatttca
    11641 ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt
    11701 acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa
    11761 cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc
    11821 gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg
    11881 gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat
    11941 ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat
    12001 ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac
    12061 caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc
    12121 gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat
    12181 ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc
    12241 agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac
    12301 aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata
    12361 aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc
    12421 ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc
    12481 caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc
    12541 tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat
    12601 tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc
    12661 gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta
    12721 taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag
    12781 actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg
    12841 tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa
    12901 aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt
    12961 ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc
    13021 gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa
    13081 gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc
    13141 aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc
    13201 aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc
    13261 atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc
    13321 tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag
    13381 atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac
    13441 tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt
    13501 cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac
    13561 aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg
    13621 gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta
    13681 tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc
    13741 ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag
    13801 ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc
    13861 gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa
    13921 caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc
    13981 ttttctggat aaccacacag ctaacagaat caagacatat accaatatca actgccaaga
    14041 taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa
    14101 tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa
    14161 tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg
    14221 actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata
    14281 gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc
    14341 taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa
    14401 aggtagaaca taataaaaca accgataata taatagccaa actactgcta catcttgccg
    14461 aacaggcaat tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg
    14521 gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat
    14581 cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca
    14641 ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa
    14701 tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa
    14761 aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa
    14821 tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt
    14881 ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc
    14941 taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag
    15001 aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca
    15061 atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt
    15121 catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat
    15181 atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat
    15241 cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca
    15301 ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc
    15361 cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag
    15421 gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg
    15481 atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt
    15541 ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg
    15601 aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag
    15661 tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa
    15721 aatcattaac agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga
    15781 tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg
    15841 ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc
    15901 ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg
    15961 tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga
    16021 cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg
    16081 ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag
    16141 gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga
    16201 cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc
    16261 cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat
    16321 ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa
    16381 tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc
    16441 gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt
    16501 gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag
    16561 ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct
    16621 acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg
    16681 attactactg ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat
    16741 ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct
    16801 aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc
    16861 gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg
    16921 attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg
    16981 cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga
    17041 tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt
    17101 ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca
    17161 gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt
    17221 tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag
    17281 cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc
    17341 tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc
    17401 atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt
    17461 tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa
    17521 ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta
    17581 cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta
    17641 ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct
    17701 aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat gattccagca
    17761 gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct
    17821 aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc
    17881 acctccatac tatctttttc atttttatca tctatacagc ttctatgata aattattcta
    17941 aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta
    18001 atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca
    18061 ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga
    18121 gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca
    18181 ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag
    18241 agtacctact cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag
    18301 gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag
    18361 ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt
    18421 tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt
    18481 tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta
    18541 aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga
    18601 ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac
    18661 gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg
    18721 gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt
    18781 ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta
    18841 acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa
    18901 ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca gcgatctcta
    18961 gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt
    19021 tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat
    19081 ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc
    19141 catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg
    19201 caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa
    19261 tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac
    19321 gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat
    19381 ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt
    19441 atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc
    19501 tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg
    19561 aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc
    19621 cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct
    19681 ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa
    19741 aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac
    19801 cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta
    19861 cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc
    19921 cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag
    19981 cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg
    20041 gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag
    20101 tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt
    20161 cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact
    20221 cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta
    20281 aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca
    20341 gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc
    20401 cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt
    20461 catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta
    20521 gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt
    20581 cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc
    20641 ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta
    20701 tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg agctgtgaca
    20761 aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc
    20821 ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc
    20881 agagttagac ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa
    20941 cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc
    21001 ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa
    21061 cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc
    21121 catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca
    21181 gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc
    21241 agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag
    21301 gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat
    21361 aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca
    21421 atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt
    21481 taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca
    21541 gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt
    21601 attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc
    21661 ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt
    21721 attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta
    21781 cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat
    21841 ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat
    21901 aaacataggc attgtctact agcacaccta accatacacg attgacagca gtaaattcaa
    21961 gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc
    22021 ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag
    22081 actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt
    22141 taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat
    22201 agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg
    22261 tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg
    22321 tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat
    22381 cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca
    22441 cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt
    22501 tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt
    22561 gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga
    22621 ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt
    22681 catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa
    22741 gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag
    22801 ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat
    22861 tagctcaaaa accggcaaaa tataaggaga tatttttccg tatgtttctc taatactcag
    22921 gtcaaaacgc tgatttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
    22981 taataagtcc aaccaagtta aaaacattaa gaagctgttt ttacttggct gttcttatta
    23041 cttgatgcag ggcagctttt tcacaacctc ttggtacacg gtgttccaga agtagcttct
    23101 tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    23161 tttcttgaag ctgaccactt ctgtcacaac ctctttgatt acttcgtttg attttatttt
    23221 aatataagtt gattaaaaga gagcatgatt tgttttattt tctcactaat ttttccaaca
    23281 caactaagaa taccataaaa gctgattttg gaggaggttc ctactacaag tctaaaacga
    23341 aatttaagac ttttcctcat aaatactggt ttattttggt ctcatgtaaa actcgctgaa
    23401 ggcctcttca cttataaatg cttccccagc tgccagtacg tctttcattt ggatcgaatc
    23461 gatgatttca ggaagatcaa ataaagttcg atcgccaaat aaactttgcg taaattgatt
    23521 ggcaatatat tcgatcgaat ttaatgattg gaaatattgt ccaagcattt tcttcttcag
    23581 caaatcgaga tttgtttctg acacttccgg atcatcagcg aatccaagaa tgatttcttt
    23641 gactttttct gctgctttct ctggttcgtc cgtatctccg ctgaagtccg caaagtgaaa
    23701 ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg ataattcctt gattgtacat
    23761 cgctagatag tttcgagaag tattgcctaa aatcatttgg aacaacagat tgattgctgt
    23821 tttatagcgt aacagttctg ttccttcttg cggcaatgtg tctaatcctt taataccaag
    23881 tacaaattta tctcgtgtaa tggctgcttc caatgcactt tgtttgatga tttcttttgt
    23941 gttttcaggg aaataacgta caatttcttg ctttggaggg aaattttttg cttcttggtt
    24001 ttcccgaata agtttcatca atttctcagg ctccatctta ccaactacaa ataaaaccat
    24061 attgcttggc tgataaaacg tccgatagca cgtatacaag tcttgagctg tgattttgtc
    24121 gatactttca accgtgcccg caatatcgat atgcaatggg tgtgtaggat acaagttatt
    24181 tagtatgcca aaaaacatcc gccagttagg gtcgtcttca tacatttgga tttcctgacc
    24241 gatgattcct ttttccttgt ttacagtttc ttctgtgaag taaggtgctt gtacaaaatc
    24301 gatcagagtc gtcagattct tttctacctg atccgtcgta gaaaacagat agctggtttt
    24361 cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc ccaaataatt gaaagacatc
    24421 accatcttct ttttcaaaca acttatgttc taaaaaatgg gcaattccat ctggaacttt
    24481 caccttttct ttttcaccat agggaataaa ttcgttatcg atcgaaccat aattcgtact
    24541 gaacaaacca tatgtcttgt gatagtcatt tttaggcaac aaatacacgg tcaaaccgtt
    24601 tggcagtact tcatgataaa gtgtttcatt gatttgctca tattctgttt tattcatttg
    24661 catttcctcc tgctaaaaag aagattgcct gcaattcgat ttgctcagct acttgttgta
    24721 tctctttgat cgttacgctc tccatacgtt tcaaccattc ttcatcagat aattttgttt
    24781 ccggcagcca gctgtccaaa aaagaagctt cgattgccgc ttgcggagag tctagagaaa
    24841 gcagatattg gtttctcagc attgcttttg tctgagacag ttccaactct gttatttcac
    24901 catttcgcaa actttctaat tgctcgtgaa tcagatgaag tacttgattt cggttctttt
    24961 catcgatacc ggtttggaca ctcatgaatc ctcggaacgt atccacgcta ctagaggcat
    25021 aataagctag actttctttc tctctgacat tcatgaaaag tttagagtga ggaaaaccgc
    25081 caaataaacc gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa tacacatttg
    25141 tctgataagc taggttcagc tttgcttgaa cgattggctc acgtacttgt cgttccttca
    25201 cgatattcac ttgaggttgg gtataaaaca gatctggata aactgtttcc cgcgactcaa
    25261 atggtaattg cccaactgct tcagctgctt tgtccggatc aacatcgccg acaacaaaaa
    25321 tgtcgatttg atcatttgcc atcatttctt gatagtagga cgctagcgaa ctagcagtga
    25381 tcttgtccaa atcttcgact acgcccaacc ctgggatctt ttgttccggg ctgttttcaa
    25441 aataaagttc ttgcaaggca taggaagcaa aagtttgttt atcttccttc aaactttcca
    25501 aatatagacg cagattgttc ttttccaagt caaacgtcaa ctgatcaaac tgatgatttt
    25561 taatatttgg ataaaataaa acctctttta aaaaatctac cgcttgagaa aaaaggtttg
    25621 gatcattgac atattcacca ttcacaaaac tgatccctgc gtttagccaa tgtaaatttc
    25681 ctttttttct aactgataat ccaaagcttg cgccataaag ctctgcaagt tgcgcactta
    25741 actttgtctg atccggataa ttcaaactat ttgtttccaa catgctgctg agaagtgaac
    25801 gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc cggatcgttt
    25861 tgtattgttt tgttggtaag acatgaagat tcacgccttc tgccaattga tacaccatat
    25921 ttatatgctc cttttcacag ttgattgccc gcttactctt ttaatgggtc aacctgcctt
    25981 ttttacttat tcctctgctt gaaatttata agttcaggca attcgccgtt ttttctaaat
    26041 gatgttacac tttatagtat agcatagatg ttttttatca aaagaaacag acggctaaaa
    26101 gcgtatattg tttttttatt ttttattttt cgatatacta aagacaaata aaagaaggga
    26161 gggtcattca tgatcaaaga attcaaagag ttcatcatgc gtggagatgt cttggatctt
    26221 gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg taaaccaagt ggtgcaaggc
    26281 ttgatcacac cactaatagg ttggtttgtc gcaatgatca caggtacttc tgatttagaa
    26341 ggagcacttt caattcttga ctggtcgccg acaaaaggcg tcacatttgc gtttggtaac
    26401 gttattagtg ctatcattac atttctgatc actggttttg tcttattctt agttgtcaaa
    26461 gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt ggaagaaact
    26521 cctactgcag aagattatct tagtgacata cgcgatcttc ttgccaaaca agtggcagaa
    26581 gacaatacac atccagtaga gcaaacaaat ctttccgata cttctaaata ataaagaatc
    26641 taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct taacttgcta
    26701 cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca tctgagattg
    26761 ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg atcacgattg
    26821 gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc tttcttccta
    26881 aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata ctctccgctt
    26941 cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc
    27001 aagcttgtcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc acaacaaaaa
    27061 ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct tcaaatttct
    27121 ttacctgata ttccactaag caaacgcccc ctgctttttt attcttagca taccgattcg
    27181 cacacaaaaa agcaaacgaa ccttacgaat ct
//
LOCUS       DAZUMI010000026        26994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000026 DAZUMI010000000
VERSION     DAZUMI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.026"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1038)
                     /locus_tag="ACG81C_000970"
     CDS             complement(70..1038)
                     /locus_tag="ACG81C_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5064148.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1270..2103
                     /locus_tag="ACG81C_000971"
     CDS             1270..2103
                     /locus_tag="ACG81C_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5064149.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2137..2976
                     /locus_tag="ACG81C_000972"
     CDS             2137..2976
                     /locus_tag="ACG81C_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5064150.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3002..3562
                     /locus_tag="ACG81C_000973"
     CDS             3002..3562
                     /locus_tag="ACG81C_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5064151.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3761..5482
                     /locus_tag="ACG81C_000974"
     CDS             3761..5482
                     /locus_tag="ACG81C_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5064152.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5647..6789
                     /locus_tag="ACG81C_000975"
     CDS             5647..6789
                     /locus_tag="ACG81C_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5064153.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6805..8016
                     /gene="thiI"
                     /locus_tag="ACG81C_000976"
     CDS             6805..8016
                     /gene="thiI"
                     /locus_tag="ACG81C_000976"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5064154.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8589..9236
                     /locus_tag="ACG81C_000977"
     CDS             8589..9236
                     /locus_tag="ACG81C_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5064155.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9365..9581
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9629..12274
                     /locus_tag="ACG81C_000978"
     CDS             9629..12274
                     /locus_tag="ACG81C_000978"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5064156.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12584..13897
                     /locus_tag="ACG81C_000979"
     CDS             12584..13897
                     /locus_tag="ACG81C_000979"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5064157.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13887..14540
                     /locus_tag="ACG81C_000980"
     CDS             13887..14540
                     /locus_tag="ACG81C_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5064158.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14595..15287
                     /gene="radC"
                     /locus_tag="ACG81C_000981"
     CDS             14595..15287
                     /gene="radC"
                     /locus_tag="ACG81C_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS5064159.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            15437..15637
                     /locus_tag="ACG81C_000982"
     CDS             15437..15637
                     /locus_tag="ACG81C_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5064160.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(15891..17570)
                     /locus_tag="ACG81C_000983"
     CDS             complement(15891..17570)
                     /locus_tag="ACG81C_000983"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5064161.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(17572..17784)
                     /locus_tag="ACG81C_000984"
     CDS             complement(17572..17784)
                     /locus_tag="ACG81C_000984"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5064162.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            18177..19907
                     /gene="efrA"
                     /locus_tag="ACG81C_000985"
     CDS             18177..19907
                     /gene="efrA"
                     /locus_tag="ACG81C_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5064163.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            19904..21664
                     /gene="efrB"
                     /locus_tag="ACG81C_000986"
     CDS             19904..21664
                     /gene="efrB"
                     /locus_tag="ACG81C_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5064164.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            21759..21956
                     /locus_tag="ACG81C_000987"
     CDS             21759..21956
                     /locus_tag="ACG81C_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064165.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            22109..22594
                     /locus_tag="ACG81C_000988"
     CDS             22109..22594
                     /locus_tag="ACG81C_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5064166.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(22698..23291)
                     /gene="clpP"
                     /locus_tag="ACG81C_000989"
     CDS             complement(22698..23291)
                     /gene="clpP"
                     /locus_tag="ACG81C_000989"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5064167.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            23471..23998
                     /locus_tag="ACG81C_000990"
     CDS             23471..23998
                     /locus_tag="ACG81C_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064168.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            24089..24826
                     /locus_tag="ACG81C_000991"
     CDS             24089..24826
                     /locus_tag="ACG81C_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5064169.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            24830..25747
                     /gene="miaA"
                     /locus_tag="ACG81C_000992"
     CDS             24830..25747
                     /gene="miaA"
                     /locus_tag="ACG81C_000992"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5064170.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            25749..26978
                     /gene="hflX"
                     /locus_tag="ACG81C_000993"
     CDS             25749..26978
                     /gene="hflX"
                     /locus_tag="ACG81C_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5064171.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8896 a         4675 c         5781 g         7642 t
ORIGIN      
        1 acttagtttt cgaagaagct atttttgtca cagcctctct ttttaagttt agctattaga
       61 taatgatgtt tagttttgga taaacgaaat caattcttca cccgacaaac catagtttac
      121 agcaatcaat agttttaaac gtgctttttg actattaata ctgttacaga aaataatccc
      181 cattttttct agttcttttc ccccgccttc ataatcataa acaggctctg cgattccatt
      241 gaaacaacga gaaacaagta ctacagggat tttttggaca agaagctttt ccaaagcagc
      301 taaggcttgt ggagggagat tccccgcacc taaagcttcg attaccaaac cgtctacttt
      361 cgtatgggcg atggcttcca ataagtcccc ttgcatccca gcatatgctt taacgatcgg
      421 gatagtccca tctacagcgg aaatgtccag tcgctttgtt tctaaaagtt cctgcaagaa
      481 taaaattcga ttcttagtta ctaagccgat gggacccaat gtaggtgttc ggaaagtagc
      541 aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa tggatctcat cgttcatgac
      601 caccaaaacc cccttatcta gcgcttcttc acaacttgcg acacgaatcg cactttcaaa
      661 attataaagg ccgtcacttc caagttcatt acttgaacgc attgccccag tcaaaacaat
      721 tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca gtttcttcta atgtatctgt
      781 accgtgtgtg atcaccactc cggatatacc tgatgcataa gcggacaaga tacgttcttt
      841 taataccaac atgtgagaag gcatgatgtg tggtgaggga agattgaaga tatcttcaac
      901 taataattct acatgcttgg ggattgtgat ttctgcatct aataatgggt ttgccacatc
      961 aggactaaca ccgcccgtga gcatgttttc tttcatggcg atcgttccgc cggtgtgtag
     1021 tacaagtatt tttttcattt tacagacttc cttacgaata attttgcata gccgttaaat
     1081 cttattgtat actatataaa agagttttgc tatcagtgaa gaataacgaa tagcctaact
     1141 tatcataaaa aagctgacct tattaaaatg gaaaagactc aaccttgacc tcctaataca
     1201 atggagtatc tggttgagtt tcttttacaa gaaagcagcc aaggaaatca atcacgaagg
     1261 tggccggaaa tgatcagaat cggattaaca tcgtttaaag aacatgacaa attaactggt
     1321 aaaaagaata acactcttta tgaatatgca gggtttctac ccttagtgga attagatact
     1381 gcctattatg gaattccccg aaagtcctca gttgagaatt ggataagtga agtacctgaa
     1441 tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg gtcaaggaga ctggaagcct
     1501 tattatcaga ccgaagaaga gatgattgcg cattttttag atgttatgtc gccaatggta
     1561 gatgctggaa agatgttttg ctttttagtc caattcccct catcattcaa atgtacgaaa
     1621 gaaaatgtcc agtacttgcg caaacttaga aagtggtttg agggttatcc tgtttctata
     1681 gaattgcggg attattcttg gtacagcgat gaatttcttg aacgtacaag aaactttatg
     1741 tctagagccg ctttttcttt agccatcatc gatgagccac agttgctgaa tacaacgatt
     1801 ccttttgatc catttgtcac caatccgaac tttgctcttt ttcgattcca tggtaggaat
     1861 gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa aaagaacgtt atatcgttat
     1921 tcagaaaaag aactgcaaga actagcacat gaagtcaaag tagttgattc taagacaaaa
     1981 gaggtaggtg tgatttttaa taataattct ggtggtgatg cggctgaaaa tgcattagca
     2041 ctgcaaaaaa tattaaatat caagccagat ggactcaatc caacccaaat ggatctgttt
     2101 taagggctta ttttattcaa gaaaggggcg aagtctatga tcaaagctat tttttttgat
     2161 attgacggaa cattggtcac caatcgttca aaagcgttgg aatcaacaaa acaagcaatt
     2221 gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa cggggcgcag cccagtaaag
     2281 atcaaagaaa tcattgatga attggaatta gatatgtatg tcgtgtataa tggtcaactg
     2341 gtttttactg cagatcgcac gattatcgac cacccttttg aacaaaaagt attggaacat
     2401 attgttgaat ttgcagatga gaaccatcga caaatcgttt ttggtgcgag aaatcggtta
     2461 gatggatcaa cgacgatgct attaggacaa tcgattttta tcaagcgact agtcagcttt
     2521 ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc tccaggtttt tagccctcac
     2581 cgacaaaagg atcgttatga aacgttagag atattaaaag aaccaattta tcaatgtata
     2641 ttgctcagtc cggagtcaga acagaaaaag ttagaacatc gctttcccga gtgtacgttt
     2701 cagcgttcaa atacgtatac tgtagatatc atcccaaaag gtggttcaaa attactaggc
     2761 atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag aaattatggc attcggcgat
     2821 cattacaatg atatcgagat gctgaaaggt gtaggaattg gagtggccat ggggaatgct
     2881 cagatcgaag taaaacatgc tgcagacttt gtgactcaat caaatgaaga agatgggata
     2941 tacgaaggac tgaaacactt cggtctgatt tattagtcaa tgaaagaagg aaaaaatcag
     3001 tatggaagca gagacacctt ttcaaaaagt agaaacattc cacaaaactt ttgatccaag
     3061 aagaccagag atacccacgg cttttacaga aaatgaagct catacacgtg ctgcgttcaa
     3121 aatagaagaa cttgttgaat ttgttgcagc tacttcacct aataaagaac aactgcatga
     3181 agctgttatg ctgctgcacg aagcattaga taaagcagaa gaaaaaatac ttggtaaaac
     3241 acagtgggga agtgatccgc tggttgaaca agtagatgca ctgacagata tcctttactt
     3301 tacttatggt tctttttcat tactaggaat agtgcctgat cgaatattcg aaatcgtcca
     3361 tcaggcaaat atggggaaat tatttccaga tggaaaacct cattatgacc ctgtaacaaa
     3421 taaagtgttg aaacctgaaa actggcagat ggaatatgct ccagaaccta aaatcgcggc
     3481 agaaatccaa cgtcagattc aagaagcgat tgctaagaag aaatccgctg atataggcat
     3541 gtgcacaaat gaaaaagagt gatcagagtt tcactatttt tgttatcttt atacgatgga
     3601 aaaacaaaag attttcgttt aagttagtga atgcatgaaa atagtatgct ataatctaga
     3661 atagatgtct tttgcacgag agacgtatta tttggattta tgccaagcag aacgccatct
     3721 gttgcataaa agatgcatac tgaaaacgga ggaaccataa atgggaaata atgttatcat
     3781 cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc tttttggtta gttacttgat
     3841 gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa cgcaaggaag aattatttga
     3901 cttgccagtg attgaagaag tcgatgatgt taaaaaaatg catctggttg ggcaaagcca
     3961 aaataccttt cgtgaatgga gtcaaaaatg gaatgacata tctacttcct cgttcgctga
     4021 actagaaagt caaattttcg aagtggaaaa cctcaatgaa agttttcggt tcttaaaagg
     4081 aaaaaaagca gttgttgaag cagaagctac aatggaccaa atggagaaac aagtgacgga
     4141 aatccgtgaa ggtctaaagg aattgcggga aacggaagaa cgcaactcac tagctgttca
     4201 gcaggcactt gacgtctatg aagaactgaa agaaaaagtc agagaagacg agcatgaata
     4261 cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg atcgaaaaag aatttacgca
     4321 gtttgtgact ttaaacactt ctggagaccc tgttgaagcc cgcgaggttt tggataaagc
     4381 agaaaaacat acgtatgaag tggaagctct gatgaaaaaa attccaccgc tgtatgagga
     4441 gctccatacc actttccctg aacaacttga agaaatcagt gatacttacg agaaattgat
     4501 ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa gatatcgcaa aagtaagtcg
     4561 aagaattgaa aattcattag ccaatcttga aaaaacagaa gttgaaacgg tcgaatttga
     4621 aaaccgtgaa acagctgatt tgattgattc gttatatgat atcttagaac gggaaatgga
     4681 agctcagaga tatgtaaaaa ctaaccaatc aacgattgca gagtatatca aacatacaac
     4741 aaagaataac cgtcaattat tgattgagct ggatcacaca tctcaaagct atacattgaa
     4801 ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa gtggaagaaa tggaacgtca
     4861 aaatgaacag atgattccac agatccgtca acacgaaatt ccttattcag aagtgcgcac
     4921 gttctataaa acagtcttca aagtcttaga agatatcgaa aagcaacaag tagagatcga
     4981 cgactcatta catgaattga gaaaaggcga aaaagaagcg caagaaaaga tcgatacatt
     5041 cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa caacgtttgc ctggcttgcc
     5101 gaatgattac ctagaattct tttttgttgc tactgaccgt attgaagaac taagtgttgt
     5161 cttgaataaa attcgagtaa acatggaaga ggtcaatcgt ttagttgctc tatgtgaaga
     5221 agatctagaa ttattagata agaagacaca tgatttagta gatgcagcag cattaacgga
     5281 gcaaatgctc caatatgcaa atcgttatcg tcatacacac gaagaagtga gagaagcaat
     5341 tgataaaagc tactatttat tcaataaaga atatcactat caagaagcat tagacgaaat
     5401 cggtacagca ttagagcgag tagaacctgg tgctttcaaa cgcatcgaag atttttattt
     5461 taatcatcca gacttagtat aaagcaaaaa agttagtaaa ccagcaagag caagacggat
     5521 tattgtcttg ttcttgtctt tttttgtata tttgaaaatt gaaacaagtg aaaatttcac
     5581 ctgctcttga aagcaccttg gacaaccgtt ataatagaaa agaatttcaa aaaagagggg
     5641 tttcttatga tatattttga taacagtgca acaaccccaa tctttccgca aagcttagat
     5701 gcatatgtaa aaaccagtca gcggattatt gggaatccat caagcttgca tgatttaggc
     5761 aatcaagcta accggttgtt gcagcaagca agaaagcaga tcgctgattt gatccatgta
     5821 gaaccagaag aaatctattt tacttcaggc ggaacagaag gagataactg ggtattaaaa
     5881 ggaacgatga tcgaaaaaag agaatatggg aatcatatga tcatttcagg agtcgaacat
     5941 ccggccgtca gcgagacagc agaacaatta aaagaattag gaatcgaagt aagtatcgcc
     6001 ccagtggata aacgcggatt tgtacgtgtc gacgaattga aagaattaat aagaaaagag
     6061 acagtattag tctctgttat ggctgtgaat aatgaagtag gatcgattca gcccatccag
     6121 gaaatcagtg atcttttgga tgattttcct aaaattcatt tccatgtaga tgctgttcag
     6181 gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa gagttgattt tgcaacattt
     6241 tcagctcata aattccatgg cccacgggga actggtttca tttattggaa aaagggtcgt
     6301 cgtttagcac cattactgac aggtggaggg caagaaagaa atcagcgaag tgggacagaa
     6361 aatgttccag gtattgtggc tatggttaaa gctttacgtt tatcattaga gataaaggaa
     6421 caaaaacctg agcagacaca acgcttaaaa gtagctctaa tcaatgcgtt gaagacgtat
     6481 gagaaagtga cgattttttc agaaggaact gactatgcac cgcatatttt atgttttgct
     6541 ctaaaaggaa ttcgcggcga agttctcgtc catgccttag aagaaaaaca aatatttgtt
     6601 tctactacta gtgcctgctc ctcacgtaaa catatggcaa gcagtacatt gcacgcaatg
     6661 catgtaccaa atgatctggc tacatctgct gttcgaatca gcttagatga aaacaattcg
     6721 cttgcagaag tcgagcaatt tatgattatt ttcaagcagc tttataagaa attttctgca
     6781 atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa ttatggtccg ttatggggaa
     6841 ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc aactagcaca aaatgtcaga
     6901 caagctttga aagactttcc agaaatcaaa atccatgctg atcgggaccg gatgcatctt
     6961 ttattgaatg gcgcagacgg gcagctagtt attccaaaac tagcaaaaat atttgggatc
     7021 caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg aagttttgaa acaagctgtt
     7081 caagacatca tgaaagaaat ttatactggg aaagaaacat ttaaaattat cgctaaaaga
     7141 agcgatcacc aatttgaatt gacaagtaat gaattgaatc aaacattagg taatgctgtt
     7201 tttgatattt tcccgcatat ccaagttcaa atgcgtcaac cagatattcc attacgtgta
     7261 gagattcggc gtgatggcgc ttatctatcg tatgaaacga tcaagggagc tggaggattg
     7321 cccgtaggaa caagcggaaa aggaatgctt atgctttctg gggggattga ttctcctgta
     7381 gcagggtact ttgccatgaa gcggggagtg gaaattgaag ctgttcattt tgctagtccg
     7441 ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc taacagccaa actagcacct
     7501 tatgtgggaa cgatccattt tatcgaagtg ccatttaccg agattcaaga agaaatcaaa
     7561 aagtcagtgc cacaaggata ttggatgaca attactcgtc gaatgatgtt gcgtttgacc
     7621 gatgaaattc gccgtattcg tcatggttta gtcattttga atggtgaatc attaggtcaa
     7681 gtggcttcac aaactttgca aagtatggtg gctatcaatg aagtaacgaa tacgccaatc
     7741 attcggcctg tagcgacaat ggataaactt gaaatcattg aagtagcaga acagatcgat
     7801 acatttaatc tttctatcca gccttttgaa gattgctgta cgatttttgc tcctccacag
     7861 ccaaaaacac gccctcaact ggaaaaggtc catcaatatg aagagcgttt agcaatagat
     7921 gaaatgatcc aacgagcact agctggcttg aagatggaag agatacaagc agaatcgtct
     7981 aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg ttatgaaaat agtgttttga
     8041 ccagagtatt agagaaacaa aacgaaaaaa tagctccatg tattttaccg tttttgaact
     8101 aatgtcgaag attagctgat tgaataccat ttatcaagaa gttatggaaa agccggttct
     8161 tttttactaa ctgaaaaata aaattttatt tagagatcaa gctacagttg ataaatgata
     8221 taaaaaagag aatttcccat gaaatagcga ggttgtgaca gaagtgggca gcttcaagaa
     8281 ataagaagga atttacgaaa attgcttttc aaatttttgt gaaatttcgg cttatttttc
     8341 gaagaagtta cttctggaac accgtgtatc agaggttgcg aaaaagctgc cctgcatcaa
     8401 gtaataagaa cagccaaaca aaaatagttc ctcatatttt acgtttggtt gagcttatta
     8461 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
     8521 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
     8581 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
     8641 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
     8701 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
     8761 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
     8821 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
     8881 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
     8941 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
     9001 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
     9061 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
     9121 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
     9181 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
     9241 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
     9301 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
     9361 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
     9421 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
     9481 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
     9541 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
     9601 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
     9661 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
     9721 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
     9781 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
     9841 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
     9901 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
     9961 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
    10021 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
    10081 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
    10141 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
    10201 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
    10261 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
    10321 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
    10381 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
    10441 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
    10501 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
    10561 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
    10621 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
    10681 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
    10741 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
    10801 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
    10861 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
    10921 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
    10981 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
    11041 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
    11101 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
    11161 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
    11221 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
    11281 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
    11341 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
    11401 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
    11461 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
    11521 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
    11581 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
    11641 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
    11701 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
    11761 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
    11821 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
    11881 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
    11941 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
    12001 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
    12061 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
    12121 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
    12181 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
    12241 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
    12301 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
    12361 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
    12421 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
    12481 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
    12541 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
    12601 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
    12661 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
    12721 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
    12781 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
    12841 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
    12901 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
    12961 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
    13021 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
    13081 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
    13141 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
    13201 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
    13261 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
    13321 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
    13381 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
    13441 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
    13501 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
    13561 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
    13621 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
    13681 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
    13741 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
    13801 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
    13861 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
    13921 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
    13981 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
    14041 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
    14101 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
    14161 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
    14221 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
    14281 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
    14341 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
    14401 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
    14461 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
    14521 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
    14581 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
    14641 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
    14701 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
    14761 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
    14821 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
    14881 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
    14941 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
    15001 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
    15061 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
    15121 ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt accaaaagga
    15181 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    15241 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    15301 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    15361 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    15421 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    15481 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    15541 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    15601 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    15661 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    15721 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    15781 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    15841 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    15901 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    15961 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    16021 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    16081 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    16141 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    16201 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    16261 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    16321 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    16381 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    16441 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    16501 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    16561 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    16621 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    16681 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    16741 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    16801 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    16861 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    16921 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    16981 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    17041 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    17101 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    17161 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    17221 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    17281 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    17341 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    17401 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    17461 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    17521 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    17581 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    17641 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    17701 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    17761 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    17821 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    17881 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    17941 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    18001 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    18061 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    18121 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    18181 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    18241 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    18301 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    18361 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    18421 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    18481 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    18541 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    18601 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    18661 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    18721 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    18781 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    18841 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    18901 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    18961 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    19021 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    19081 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    19141 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    19201 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    19261 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    19321 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    19381 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    19441 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    19501 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    19561 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    19621 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    19681 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    19741 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    19801 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    19861 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    19921 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    19981 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    20041 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    20101 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    20161 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    20221 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    20281 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    20341 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    20401 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    20461 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    20521 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    20581 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    20641 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    20701 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    20761 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    20821 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    20881 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    20941 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    21001 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    21061 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    21121 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    21181 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    21241 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    21301 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    21361 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    21421 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    21481 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    21541 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    21601 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    21661 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    21721 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    21781 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    21841 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    21901 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    21961 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    22021 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    22081 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    22141 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    22201 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    22261 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    22321 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    22381 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    22441 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    22501 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    22561 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    22621 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    22681 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    22741 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    22801 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    22861 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    22921 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    22981 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    23041 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    23101 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    23161 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    23221 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    23281 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    23341 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    23401 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    23461 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    23521 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    23581 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    23641 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    23701 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    23761 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    23821 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    23881 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    23941 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    24001 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    24061 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    24121 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    24181 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    24241 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    24301 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    24361 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    24421 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    24481 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    24541 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    24601 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    24661 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    24721 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    24781 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    24841 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    24901 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    24961 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    25021 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    25081 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    25141 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    25201 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    25261 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    25321 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    25381 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    25441 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    25501 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    25561 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    25621 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    25681 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    25741 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    25801 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    25861 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    25921 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    25981 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    26041 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    26101 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    26161 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    26221 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    26281 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    26341 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    26401 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    26461 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    26521 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    26581 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    26641 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    26701 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    26761 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    26821 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    26881 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    26941 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DAZUMI010000027        26188 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000027 DAZUMI010000000
VERSION     DAZUMI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.027"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..1100
                     /locus_tag="ACG81C_000994"
     CDS             96..1100
                     /locus_tag="ACG81C_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064172.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1101..2045
                     /locus_tag="ACG81C_000995"
     CDS             1101..2045
                     /locus_tag="ACG81C_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064173.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2045..3007
                     /gene="opp4B"
                     /locus_tag="ACG81C_000996"
     CDS             2045..3007
                     /gene="opp4B"
                     /locus_tag="ACG81C_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5064174.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3034..3948
                     /locus_tag="ACG81C_000997"
     CDS             3034..3948
                     /locus_tag="ACG81C_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064175.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3974..5755
                     /locus_tag="ACG81C_000998"
     CDS             3974..5755
                     /locus_tag="ACG81C_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5064176.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6103..6789
                     /gene="rnc"
                     /locus_tag="ACG81C_000999"
     CDS             6103..6789
                     /gene="rnc"
                     /locus_tag="ACG81C_000999"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5064177.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6809..10390
                     /gene="smc"
                     /locus_tag="ACG81C_001000"
     CDS             6809..10390
                     /gene="smc"
                     /locus_tag="ACG81C_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5064178.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10399..11208
                     /locus_tag="ACG81C_001001"
     CDS             10399..11208
                     /locus_tag="ACG81C_001001"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5064179.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11219..12217
                     /gene="ftsY"
                     /locus_tag="ACG81C_001002"
     CDS             11219..12217
                     /gene="ftsY"
                     /locus_tag="ACG81C_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5064180.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12404..14671
                     /gene="gshAB"
                     /locus_tag="ACG81C_001003"
     CDS             12404..14671
                     /gene="gshAB"
                     /locus_tag="ACG81C_001003"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5064181.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14760..15212)
                     /locus_tag="ACG81C_001004"
     CDS             complement(14760..15212)
                     /locus_tag="ACG81C_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5064182.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15362..16312
                     /locus_tag="ACG81C_001005"
     CDS             15362..16312
                     /locus_tag="ACG81C_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5064183.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16305..17264
                     /locus_tag="ACG81C_001006"
     CDS             16305..17264
                     /locus_tag="ACG81C_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5064184.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17261..18016
                     /locus_tag="ACG81C_001007"
     CDS             17261..18016
                     /locus_tag="ACG81C_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064185.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18039..18992
                     /locus_tag="ACG81C_001008"
     CDS             18039..18992
                     /locus_tag="ACG81C_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5064186.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19636..19932)
                     /locus_tag="ACG81C_001009"
     CDS             complement(19636..19932)
                     /locus_tag="ACG81C_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS5064187.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20287..20643
                     /locus_tag="ACG81C_001010"
     CDS             20287..20643
                     /locus_tag="ACG81C_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5064188.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20653..21552
                     /locus_tag="ACG81C_001011"
     CDS             20653..21552
                     /locus_tag="ACG81C_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5064189.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22072..22800
                     /locus_tag="ACG81C_001012"
     CDS             22072..22800
                     /locus_tag="ACG81C_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5064190.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22945..24972
                     /locus_tag="ACG81C_001013"
     CDS             22945..24972
                     /locus_tag="ACG81C_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5064191.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25080..26177
                     /locus_tag="ACG81C_001014"
     CDS             25080..26177
                     /locus_tag="ACG81C_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5064192.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         8739 a         4567 c         5559 g         7323 t
ORIGIN      
        1 ataagaataa cctttgttat aaaacgcttt attctgtgta atgtcctttt ttattaagaa
       61 aaacttccat ttaggaagca gagaggggta ttcgattgtc cgataaccaa ttattagaag
      121 ttcagaacct acatacagga ttccgactca aagatgacta ttacgatgca gtagatgacg
      181 tttcatttac tttagacaaa aacgagattt tagcaatcgt tggggaatct gggtgcggga
      241 aaagtacctt ggctactacg attatgggat tacttgatcc taataacaca aaaattacag
      301 gtgagatttt atataacgat ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa
      361 tccgcggaaa tgatatcgga atgattttcc aagatccgtt gtctgcatta aatcctctca
      421 tgagaatcga agaccagatc aaagaaggac tttcctatca cacaaagatg aatgcagaac
      481 agcgtcaagc tcgtgcacta gaattgctag aacaagtagg gattcctaat cctgcgagag
      541 ttggacgcca atatccccat gaactatcag gcggtatgag acaacgtgtc atcatcgcaa
      601 tcgcaatcgc ctgcaaacct ccaatcgtca ttgcagatga accgacgaca gccctagatg
      661 taaccatcca agcacagatt cttgatttac tgaaagacct tcaggaagaa acaaaaacag
      721 ggatcatctt gatcactcat gacttaggag tcgtagcaga aatggctgat cgagtggcag
      781 tgatgtatgg cggacagttc gttgaagtag caggtgtaaa agaactgttt gatcatccta
      841 agcatcctta tacacgctct ttactgaact ctataccaca agaaggaaat catgatgcag
      901 aactccatgt gatcgaaggt gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc
      961 ggtttgcacc acgtattcca tggataccag caagcgatca tgaagaagag cctatccttc
     1021 atgaagtcgc accgaaccac ttggtacgtt gctcctgcta taagcatttc cactttagag
     1081 acgaaaaagg agaggtgtag atgacagaat taatcacaat caaagacctg aaagttcatt
     1141 accctattcg cagtggattc ttcaatcgag taacggatca tgtatatgct gtagatggtg
     1201 tggattttat tatcgaaaaa ggaaagacat atggtttagt gggagagtct ggctctggaa
     1261 aatctactac gggaaaagcc gttgtgggat tagaaaaggt aacagccgga caaatcatgt
     1321 atgaaggaaa agatgtaacg aagcgaagca accgcaaaaa aatgggttat aacaaggatg
     1381 tgcagatgat cttccaagat tcaatgtcca gtttgaatcc taaaaagcga gtgttggata
     1441 tcattgctga acccatccga aattttgaac ggttaagcga tcaagaagaa aagaaaaagg
     1501 taaaaggact gctagatatc gttgggatgc cggaagatgc gctatataaa tatcctcatg
     1561 aattttcagg cggacaacgt caacgtctag gagtagcccg tgctgttgct accaatccaa
     1621 aactgatcgt agccgatgaa cctgtatctg ctctggatct atctgttcaa gcgcaagtat
     1681 tgaatttcat gaaacgaatc cagcaagaat acggcctcag ctatttattc atttcacatg
     1741 atttaggtgt agtaaaacac atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg
     1801 tagaaatcgg tacaagagaa gacatttata atgacccgcg acatatctat acgaaacgtc
     1861 tgctttcagc tattccgcaa atcgatgtag aaaataggga aagccacaaa gaaaatcgac
     1921 gaagagtaga acaagagtat atccaaaacc aaaaagaata ctatgatgcg actggtcgag
     1981 tctacgattt gcgtacgatc acaactacac acaaagtggc gttgaaagat ggaggtgcaa
     2041 gctaatgtgg aaaacgattc tgcgacgtgt tttattgatg atcccgcaag ttattatttt
     2101 aagtgttctg atcttcttat tagcccaagc gatgccgggg gatccattta ccggtttgat
     2161 caatccaaat caggatccgg cagtgatcga acagatgcga caagcagctg gcttgaatga
     2221 cccatggtat gtccagtact tccgttggat cggaaatgct ttgcaaggcg attttggtca
     2281 aagtttctta tacaaacgct ctgttgctga cttgatcggc gaacggatca tcaacacttt
     2341 atatctatcg atcttgacag ttgtccttac ttatttgatt gctgtgcctc tggggatgct
     2401 tgctggccgg tatcaaaatt caatcttgga taagattgtc gtaatctaca acttctttag
     2461 ttttgctgtt cctttattta tcttcggctt gatcatgcta tttgtttttg gttatcgttt
     2521 aggctggttc ccaacaagtg gatcacaaac actaggtttg caaggtggat ttatcactca
     2581 atggatagac cgtttgaaat atattctgtt accatcaatt acccaagcat ttttggcgac
     2641 tgccgtcaca atccaatatc tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt
     2701 cagaacagcc cgttccaaag gggtaccgac gaataaagta ttcagtcgac atattttccg
     2761 aaatgcagcg ttgccgatgg cttctcaaat ggggtatgaa atcacttcgt tgattgctgg
     2821 ttctgtagtt atcgaaaaga tttttggtta tccgggagtc ggaaaactat ttattgattc
     2881 catcggccaa cgggactata ccgtgatcac atccttagta ttgattttag gaatcgcgac
     2941 gctagtgggg acattacttt cagatatcat catgagtatc gttgacccgc gtattcggat
     3001 tcaataaaac caatcagaaa ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt
     3061 caagaaagta ttcctcctat ggggatacga atgattgctc gagaatttaa aaaagataaa
     3121 attgctattt tttcattagg tttgctagtg gttttattat tagtgatctt tatcggtgcg
     3181 atgctgaccg atcaagacaa agtgatgtat gttagtatct tcgacaaata cgctgcacca
     3241 ggaacagttt cgacacaagg acaaaagttc attctgggag cagatgaagg cggacgtgat
     3301 gtattaggac aattgatcat aggtgctaga aactctgtta cgatcggatt tgcgatcacg
     3361 atccttactt cgatcatcgg ggttggatta gggatcatct ctggctacta cggagggatc
     3421 attgacaaca tcttgatgcg gatcgttgat tttatcatga ttctaccgat catgctgatc
     3481 attatcgtct tcgtatcgat tatttccaga tacaatgtct ggtcctttat attcatcatg
     3541 agtgcatttt attgggtagc taaagcccga ttattccgaa gcaagacatt atctgaagca
     3601 agaagagatt atgtcagtgc ctcaaaaaca atgggaacca atgattttaa aatcatgttc
     3661 cgcgaaatca tgccgaatct aagttcatta atcattacta atttgacgat gaactttgcg
     3721 gcaaacatcg gtatcgagac aactttgact ttcttaggtt tcggcttgcc ggcaaatgta
     3781 cctagtttag gtacattgat cggttatgca agtaacggag aagtcttagt gaatcggcaa
     3841 tggatctggt tacccgcatc aatccttatt ttagttttga tgttgagtat caattatgtt
     3901 ggacaagcat tcaagcgttc tgcagatgcg cgccaacggt taggctagta tagaagcgaa
     3961 gggggaaata ggaatgaaga aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt
     4021 tcttgcagca tgcgggggcg gaggtacaga tacctctgga aacaactctg gaggcaacgg
     4081 caataacaaa gaggaattaa cttttccaat tgcaacaaaa aataatgatg atgcaatcga
     4141 agacggcgaa ttagaagctg cggtcgcaac ggacagtcag tttaaaggat tgttccagtg
     4201 ggagttttac caagattcat atgatgcagc ttttatggct ccttctcatg aaccgctatt
     4261 tgcaaacgat gacgatttca gaatcacaga cgacggagct gcaacgcttg atctagatga
     4321 agatacgaaa aaagcgacaa tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga
     4381 tgtaacggcg gacgatgtga ttttccctta cgaaatcatt gggaataaag attacactgg
     4441 tatccgttat gacgacactt tccgtaacat cgtaggtatg gaagaatatc atgatggaag
     4501 tgccgacaca atctccggaa tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa
     4561 agaagtaaac ccaagcatgc ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa
     4621 acatacgtta gaaggtgtag caatcaaaga tatggaatca agcgatcaag tacgtaaaaa
     4681 tccagtgact tttggccctt attacatgag taatatcgtt gcaggagaat cagtagaata
     4741 cttgccaaac gagcactact ggaatggtac gcctaaattg aaaaagatca cgatgaaatc
     4801 tgtaccgatg gcaagtgcaa ctgaagcatt gaactctaaa ttatatgata tgattttcca
     4861 aatgccaaca gatacttacg atacctataa agaaattgct ggctatacaa acttaggccg
     4921 tgaacaaact tcttatactt atctaggatt caagttaggt aaatggaatg cagatacaaa
     4981 gagtgtggaa tacaatccag attcaaaaat ggctgataaa tcattacgtc aagcaatggg
     5041 ctatgcatta gataataatg cagtaggtga acgtttctat gcaggtttga gaagcaatgc
     5101 gacttcattg attcctccag tattccagtc ataccatgat gatgagatga aaggattcac
     5161 acaagatcta gacaaagcga aaaaactatt agatgatgct ggctacaaag atgtggatga
     5221 cgatggttta agagaagaca aagatggaaa taaactaaca atcaactttg cttcgatgtc
     5281 cggtggggaa acagcgcaac cattggcaga ttactatatc caacaatgga aagaaattgg
     5341 tttggatgtc caatacacaa caggtcgtct gatcgaattc aattctttct atgataaatt
     5401 agaaaacgat gatcctgaaa ttgatgttta ccaagctgct tggaatacag gaactgatcc
     5461 aaatccaagt ggactatggg gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga
     5521 agaaaataca aaattgatca acgatatcag ttcaagcaaa gcatttgatg aagagtacca
     5581 aaaagaagca tatcaaaaat ggcaacaata tgcatttgaa gaagcatttg cgattccaac
     5641 gttgttccgt aatgaagtat tgccagtcaa cgaccgtgtc aaagactggg attggtctta
     5701 tgatgtacca aacccatggg caaatgtttc tgttacagca gattcacgtt cataatcact
     5761 aaaaatgacc gaaaaagggc gatgtccttt ttcggttttt tgatattttt ctgtttattt
     5821 ctctaatatt caggtcaagg cgctgatttc atgacttctc caataaacgg tgttcaaagt
     5881 ccagtttctc ggtattaagt caaatttatg caaaaatatg gaaagccatt ttcacaaaat
     5941 tttttcttca tactcgaaac taaacgaatt tttcacaact tctcgtcaat cactaggatt
     6001 attaaagctt tacttacctt ttgaccgaac catttcaaat ttgacaaaaa accagtataa
     6061 taaggaagta tcgaaaaaat atccataaaa ggaatgtgga aaatggacaa ccagctgaca
     6121 aaagaactaa aagaaaacta taacatcgtt tttcacgatc taaatttgct tgaacaagct
     6181 tttacccatt catcctatgt gaatgagcat cgcaatctgc aattatcgga taatgagcga
     6241 cttgaatttt taggagatgc cgttttagaa ctgatggttt ctcagtattt attccgttta
     6301 tatcctcatt tgccagaagg aaaattgaca aaaacacgag ctgcgatcgt tcgcgaagac
     6361 agcttgtcga aatttgccaa agaatgtcac tttgatcaat atgttttgtt gggcaaggga
     6421 gaagagaatt caggaggacg tacgcgacca gcattgcttt gcgacctatt cgaagctttt
     6481 ttaggcgcgt tatatcttga ccagggattc gacacgaccc atgcattcat cgaaaaagtg
     6541 atttttccta aagtcaaagc aggtgctttt tcacatgaga tggatcataa aacaaaacta
     6601 caagaagtct tgcaaaaatc aggtgatgta tcaatcgaat accgattgat caatgaagaa
     6661 ggtccagctc atgaacgagt attttggatc gaagtttatg tagatgatca attgattggc
     6721 actggacaag ggaaatcgaa aaaattagca gaacaagctg cggcggaaaa tgcattggca
     6781 gcactgtaaa agtaagtgag ggaaagcttt gtatttgaaa cgaattgaaa tagccggttt
     6841 taaatcattt gcagacaaga cagtcattga ttttgaaaat agcgtcacag cagtggttgg
     6901 accaaatggc agcggaaaaa gcaatattac agaggctatc cgctgggtat taggagaaca
     6961 atctgctaaa agtctgcgag gcggaaaaat gccggatatc atctttgccg gttccgatac
     7021 ccgtaagcag ctgaatattg cagaagtcac agtgatttta gacaatagtg atcattatct
     7081 gccattggag tacaatgaga tcagtgtaac ccgccgttat cgacggactg gggaaagcga
     7141 gtttttcatc aataaacaaa gctgcagatt aaaagatatc caagaattgt tcttagattc
     7201 tggattagga aaagaatcgt tctcaatcat ttctcaagga aaagtagaag cgattttcag
     7261 tagtaaaccg gaagaccgtc gtgggatctt tgaagaagca gctggtgtct tgaaatacaa
     7321 gcagcggaaa aagaaagcag aacagaagct ttttgaaaca gaagacaacc tcagccgcgt
     7381 ccaagacatc atccacgaat tggaagaaca gttaactcct ttggccgccc agagtgaggc
     7441 agcaaaggag tttttacggc taaaagaaac actgactcaa accgatgtct ctttgatggt
     7501 tgctgaaatc aagacagcaa aaaaagattg ggacaacaaa caagcacagc ttgcgaaatt
     7561 caatctagaa ttaggcaaac tatctgagtc gatccaagaa caagaatcca ttttggcgaa
     7621 gcagcgtaaa gaaaatgcgc aagccgatcg tctgatcgaa aaaaatcagc aagtattatt
     7681 agatctttcg gaaaaactga agcagacaga aggacaaaaa gatgttttgc aggagcggac
     7741 aaagcataca caaaaaagct cgcaagaata tcaaacttca ttagcagaag cacaaaaaaa
     7801 ggttaaacat tttgaaaagc ttcaagaaag cttgatgaaa gcagcagcag aaaaagaaac
     7861 agagatccaa aaagctgaag caaatctcat aaaaacacag caagaattag aaaaatacca
     7921 aaaatcaacg aaagaattat tggcagaatt aagagatcaa tatgttgatt tgatgcaaga
     7981 acaagcagct gtaggaaacg aactgaagta tctagaacgt caatatatac aagaaacagc
     8041 aaaaagcaag cagacgttag ccaagcaatc ggaagtagaa gcatcagtag accgattgat
     8101 cttacaaaaa gaagaattga cacaaaagca ggcgcagcta aaatcatctt taactgaaac
     8161 aaaagagaag ttagaaatga tccaacaaaa cgggaaaaag ttccaagaag cattagcaaa
     8221 agaacagcct aaaatgtatc aattaatgaa tcaagtccag cagctacgtg ctcgtaagaa
     8281 aagccttcaa gaaatccaag aaaactattt tggtttttac caaggggttc gattggtctt
     8341 gcagcataaa cagcagcttt cgggtattgt cggagcagtg gctgaactga tcgatgttcc
     8401 tgcagatttc acgttagcta tcgaagcagc tttaggtggg gcagcccagc atgtcatcgt
     8461 tgaaaatgaa aaagatgctc ggcaagccat cacgtatttg aaacagcaaa gaggaggaag
     8521 agcaacattc cttccactga cgacaatcaa accaagacaa ttaccggctc atatattgac
     8581 acaagcagca gctgtagaag ggtttatagg gattgccagt gaacaagtca gttatcccga
     8641 tcaaatccaa actgtggttc aaaacctgct aggaacgatc cttttagcca aagacttaac
     8701 tagtgccaat gcaatcgcgc aaacgatccg ctatcaatat cgagtggttt ccttagaagg
     8761 tgatgtgatg aatgccggag gatccatgac aggtggagcg aataaacgag gaaaccaagg
     8821 cagtttgttt gtacaaaatc aagaattgaa acagttgact agtgaatttg aagaagcaga
     8881 taaacaactc caagcacaag aaaaaaaagt tcaggagctg caacaagaaa cagctcgttt
     8941 agccgaagaa caagaagttt tgcgtacacg aggcgaacaa ttacgttttg aagaacaaga
     9001 agcgacaaat cagctacaaa acatcataaa tgaacttgaa cgttttgaaa aagaaaagca
     9061 aatctcaact tttgagacac gtgaactgca gcagtttatc gaagattacg aaaagcaaac
     9121 gaacgaattg aaagacaaac aaacagatct tgaatcgcaa cgacaaaaaa tcgacgaaga
     9181 aatcaaatcg ttaagtcaag aaagcgacca gatggaagcc agaagagcac aagtacaaag
     9241 tcaaaaagca caagaacaag cagatcttgc tgtcttgaaa gagcagttta atcatctgca
     9301 gatccagctc cgcggtgccc gagtccaaaa agcagaggca accgaacgtc aagaagcgat
     9361 agaaaagcag ttggcgacat tgacagcaga cttttctgat catgaagtca cagaagaaag
     9421 tttggaaaaa caaattaacg aattatctgc tcagcgagaa acattaaaag ccgagttggc
     9481 aaaagccaaa gagcagcgag atagaaggca aaaggagatc gataagcttg aagcagtctt
     9541 ggcagagcgt aatcgtgagc aaaaagcacg actgtcggaa caatccaaat tagaagtcca
     9601 aaaagaccga gctgaaatga ttttagataa tcatttgacc tatctgcagt cagaatatca
     9661 gctgacattt gagaaagctt cccaagacta tcaagaaaca accgatatcg aagacagtag
     9721 aacaaaggtt tcatccttaa aagaacagat tgaaaaactg ggaccagtaa acttaaattc
     9781 aatcgaacaa tacgaacaag tcagtgaaag acatacattc ttagcaacac agcgagatga
     9841 tctgttagca gcgaaaaacc aattgtttga aacgatggat gagatggatg atgaagtgcg
     9901 gacaagattc aaagaagtat ttgaagctat ccgtcaagaa ttcaaaattg tctttccaaa
     9961 tatgtttggc ggcggtaaag ctgaattagt actgacggaa ccaactgatt tgctgaaaac
    10021 aggtattgag attgaagtac aacctcctgg caaaaaactg caaagtctaa gtttgctttc
    10081 tggaggagaa cgagcactta cagccatcgc gttgctcttt tccattatcc gtgtctgtcc
    10141 tgttccgttt tgtatcttag atgaggtaga agcagcgtta gatgaagcca acgtgaaacg
    10201 ttttggtcgt tacttgagcg atttccaaga tgacacacaa tttatcgtag tcacacaccg
    10261 caaaggaacg atggtcgcag cagatgtact gtacggagtg acgatgcagg aatccggtgt
    10321 atcaaaaatc gtttctgtac ggatggaaga tatcaatgaa gaggcgaaaa tcaacgaaac
    10381 gaaagtgtag gaacgaaaat gatcaaatta gtagcaatcg atttggacgg tacattatta
    10441 aatgaacaaa agctgatttc agatgagaat aaacaagcat tagcacaagc aaaacagcaa
    10501 ggggtcaaga tcgttctatg tacaggacgc cccttagctg cgatggcgca ttatctgcaa
    10561 gaattaggac tggtagatga aggtgactac agcatcactt tcaacggcgg tttggtacaa
    10621 aaaaatgata ctggtgaaat catggaaaag aaagtgatgg aagtatcaga tatccaacgc
    10681 ttatacaaac tggctcagca attggatctg ccactagatg ttttgtctga caatgtggtt
    10741 ttacagctgc ctagtgcccc aaggaagcaa tccttgtaca atgtgttgaa taagctgctg
    10801 caattccaac cagcggatat aagcgatatt acagatgaat ttgtgcttaa taaggcagta
    10861 atcgcttatg accaagaaga attagatcct aaaatcaaag aaataccagc agaattccat
    10921 gaaatgtatg agatcatcaa aacaagaagt atgcttttgg aatttatgcc aaaaggtgtt
    10981 acaaaagctt atggtatttc tttattggca aaagatcttg gtttgaaaca agaagaaatc
    11041 atggcgatcg gtgatgagga aaatgattta ccgatgatcc aatatgctgg actaggcgta
    11101 gcgatggaga atgctgtgcc tttcgtcaaa gaagcagcag atttcgtgac agccagcaat
    11161 gaagaaaatg gcgtagccaa agtgatccaa aaatttgtat tagagtaggg ggagtcaaat
    11221 gggatttttt gataaaatca agcgtgcttt tactggcgaa gagaaaacag aagaagttga
    11281 agtacaggag aagtacgaca agggtttaga aaagacgcgt aaaacatttg gtgaacggat
    11341 gaatgagttg tttgccaatt ttcgaacagt cgatgaagac ttttttgaag aattagaaga
    11401 aacacttatc ggtgcagatg taggttttga aacagcgata cgaatcaccg aagcattacg
    11461 tcaagaagtg aaactcagaa atgcgaaaaa atcggcagaa gtccaaaatg cgatcatcga
    11521 gaaacttgtg gatctctatg aagcggaagg aatcaatgaa gtaaatgaac taaatatcca
    11581 agaagacgga ctaacagtta ttttgtttgt cggtgttaat ggtgtaggga aaacaacaag
    11641 tattggtaaa cttgcccatc agttcaaaaa cgaaggtaaa aaagtattga tggcagcagc
    11701 agataccttc cgcgctggag caatcgacca gttagttgtc tggggcgaac gcgctggtgt
    11761 tgaagttgtt cgtggcaatg caggaggcga tccagcagct gtcgtattcg atgctttgga
    11821 aagagcaaag acagagcagg cagatgtttt actagttgat actgcaggcc gactgcaaaa
    11881 taaagtgaac ttgatgaacg aattagaaaa aataaaacga gtaatccagc gggaattacc
    11941 agaagcaccc caagaaacac ttcttgtatt agatgcaacc actggtcaaa acgcgatggt
    12001 tcaagcaaaa caattcaaag aaacaacaga tgtgaccggt cttgttttga caaaattaga
    12061 tggtacagca aagggcggta tcgtacttgc tatccgtaat gaattgcatt taccagtgaa
    12121 attggtcggt cttggtgaag gaatcgatga cttggagccg tttgatccaa atgattttgt
    12181 cgttggattg ttcaaaggat tactaaaaga agaataaccc atgtctaaaa agcaggagct
    12241 gtgacaaaag ttgcagctcc ttgtttgaat gattgttttt aacatagctg tgtttttgat
    12301 gccttcattt ttatacttat ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga
    12361 ctttttgatt ataattagat tcatatgaat tggaggtcac ttaatgatga attttaagca
    12421 attattattg catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg
    12481 ggaaggacag cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt
    12541 tggcgatcga tcctatcacc cctatatcca aacagatttt agcgaaacac aaacagagat
    12601 gatcacccct gttaccgatt ctattcccga attatttcag tatctcgctg ctgtttatga
    12661 tgtgactgct cgttctatac cgaaagaaga gatgatctgg ccattaagta tgccacctgc
    12721 cttaccggaa aaagacgaag agatcattat tgcaaaatta aaaaatttcg aagatgtctt
    12781 gtatcgacgt tatttagcaa aaaaatacgg gaagcgaaaa cagatggtaa gcggtattca
    12841 tttcaatttt gaatttggcg atgagttgct aagaacattg ttcagccatc aggaagaatt
    12901 tcaagatttt tccgaattca aaacagaact ttatttgaaa acagcgagaa actttatgcg
    12961 ctatcgctgg atgatcactt atttattcgg tgcttcacca atgagtgaga agaactactt
    13021 tttagatgaa tcacatccgc aagaacctgt tcgcagtatt cgcaacagtg cgctgggcta
    13081 tacgaatcat ccaaatgtga aagtttccta tgcttctatg aaacagtatt tagcagatat
    13141 cgagcgaatg atcgaagaag ggaaactttc ggaggagaaa gaattttata caccgcttcg
    13201 tttccgaggc ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt atattgaatt
    13261 gcggaatatc gatttgaatc cttatgcaag attgggtatc aatccagagc aagttcggtt
    13321 cttacaatta ttcctgatgt acatgttatg gacagaagaa aaagaagact gtgaccaatg
    13381 ggtagcagaa ggtacgactc gaaataacaa agtagcacta gaacaaccat cagatcaaac
    13441 agaatttcat caagaaggta gagaaattct tgaaggaatg aagcaaatgc tggttgaatt
    13501 ggattggttg gattctcttt atttagtaga ggaagcattg actcaaatgg atcatcctga
    13561 acaaacgtta gcagcgaaac tctatcaaga agcacagcta tcgagtcagc aagaagttgc
    13621 tgtggcattg ggacatcaat attataaaga aagccatgaa cgtccctatc agttggccgg
    13681 ttttcgcgaa atggaacttt ctactcagat atttatgttc gatgccatcc aaaaaggtgt
    13741 ccaagtcaaa gtattagatg agtcggatca atttttgcgt ctgcaattcc aagaccatgt
    13801 agaatatgtg aaaaatgcaa acatgacaag taaggacagc tatatcgtcc cacttatcat
    13861 ggaaaacaaa acagtaacga aaaaagtctt aaaagaagct gggttccggg taccaggagg
    13921 cgcagaattt tcatccatgg aagaagcggt aaaagcttat ccgagatttg ccgagcaggc
    13981 atttgtgatc aaacctaaat caaccaatta cgggttaggt atcacgattt tcaaggaagg
    14041 agctagtctg gaagattatc aagcaggttt agccattgct tttcgtgaag acagttcggt
    14101 tttagtagaa gaatttatgc cgggaacgga atatcgtttc tttgtgatcg acggagaagt
    14161 ccaagccatc atgttgcgag tccctgctaa tgttattggt gacagtatcc gaacagtgaa
    14221 agaacttgtt gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt
    14281 agaattgatc caattaggtg aattagaaca attgatgttg aaagaacaag ggttgacgat
    14341 cgaatcagtt ccccaagcca atcaaattgt ttatctaagg gagaactcaa atatcagtac
    14401 aggtggtgat tcgatcgata tgactgatga attttctgaa gcttacaaaa aaatcgccgt
    14461 ttctgcggta gaagcactag gagctaagat tagtggtatc gatttgatta taccagataa
    14521 agaaatcgat cctacaactg ataaaaaagc atatgggatc atcgaagcaa atttcaatcc
    14581 agccatgcat atgcatgttt atccttttgc aggaaaaggc agaagattga cgatgaatgt
    14641 attgaaattg ttatatcctg aagtatttta aaagaaaaag cttttgacag attgtcctat
    14701 ttgatgtcaa tctgatcaaa agcttttttt gcatgtaaaa atttatgtgt tttttcgttt
    14761 taaaataaac gatcccagaa gctttttttc ttttttggtt tttctttttc agctggcgaa
    14821 gagttttctt ctgggtattt tttttcgata tcaatcacag gttcatttac tgcttctttt
    14881 gctgttaaga gcaatccgaa ttcctcagga tttgcagtga cgtaatcgtt gactacagtg
    14941 aaacggctat tgtttttcgt tgctagctgg atatattggt tttggacaga aaggggtaga
    15001 gccccattca agagtatgga agcatctgga tgttttttga gttcttttaa aaagttttct
    15061 ttgttttttg gttggatcat ttcagcaaca gtcatactaa gatagcagcg ttccctgaat
    15121 gtccctaaat atttatgctg ttcttcaggg ttcactaaag gtgtaccata aagtccctta
    15181 tccaaatggc tttttacatc atcttgtgcc ataaaaaata ccctccttct tctctttgtc
    15241 tttcttacga ttatagcata ttcccaataa tacctaaaac atatgaaaaa gcggttgctt
    15301 ttgttcaacg attcacttaa aataagaatg aaaatcattc tcaattagaa aggtggattg
    15361 gatgcgctac ggacgactag tagcattatt agttatttta agtagtattt cattgttcat
    15421 cggagtatcg aatgtacaat tgtcagaact gctccagtta gatgggcaaa gcttacaaat
    15481 cttgctagtg actcgtttgc cacgaacgat ttgtttgatc cttgtgggag cgaccagtag
    15541 tatttgtggg ttgatcatgc aacatttgac ccaaaataaa tttgtttcac caacgactgc
    15601 gggcacgatg gatagtgcgc gtttaggtat tttagtggct atgattttcc tacctggtgc
    15661 atccttgatt gtccgatcct tgacagcatt ttgctttgct tttgcaggaa cattgctttt
    15721 tttatccctt acgcgattgt tcctccagaa aaatcaattg atgcttccgt tgattggagt
    15781 catgttaggg aatatcattg gttcagttgc tacttttttt gcttatcaat tccagttggt
    15841 acaaaatatg acttcatggc ttcaagggaa ttttgcgact gtgatacgag gaaattatga
    15901 gttattgtat gctgttgttc cattgttgct cttggtttgg ctatttgctt accgatttac
    15961 gatcattggt ctgggcgaat cgtttgcagt aaatctggga atagattacc agaagatgca
    16021 gtttttaggc attggcttag tatcattggc tagtgcagtc atgttgatca tggtaggaag
    16081 cattcctttt atcggagtga tcattccgaa tcttgtctct agaatatatg gagatcaagt
    16141 gcacaaaaca attggcgtaa ctgctttagc aggtagtttg ttcttagtat tttgcgatct
    16201 tgtcgctcgc gtgttgattt ttccttatga aataccagtc agtgtgatcg ttggaatcat
    16261 cggtggggct atcttccttt atcttctgat aaggggacga cgatatgctt aaaaaaaagc
    16321 catctatttt tattggtatg cttgttcttg ttttatttgt tacaggaatg tacttgacga
    16381 tccatacata tggaaactgg cattttgcat gtgcattgag gagtaagaaa atttttgcat
    16441 ttatccttgt ctcgctagct gtcagcagtg ccacaatcag ctttcagaca attacgcata
    16501 accagttttt gactccttca gtattgggac ttgagtcgtt atatgtcctt gtccaaacgg
    16561 tccttttctt ctttatcgga ggagtgacga tgcttcagca gacaggtaca gtttttttct
    16621 tattgaacgt tgggctgatg gtcgggctga gtctattgtt atttttcttt cttttgaaaa
    16681 aaatgagagc gaatcttttt ttattactga tgtcagggat gattttaggt acgctttttg
    16741 gtagtgtcag taccttttta caagtcatca tggatccgaa tgaatatgat ttgcttcaag
    16801 gcagattatt tgcaagcttt ggcaatatcc agagtcagta tcttttgcct gcttctttga
    16861 tcattatcgg ctgctggtac ctgttatttc gatatcgaca tgtgctagac gttttccatc
    16921 tcggaaatga acaagcagta aacctcggga tagacacaga tcgttttgaa tttttgattt
    16981 tatgttgtgt cagtcttttg acagggacat caacggctct tgtgggtcca gttacttttt
    17041 tagggtttat tgtcgcaaat atcagttatc gtgtcttttc tacttatcgt catggtcagc
    17101 tatttatcgg aagctttctg gtagctatgc tgtttctgac aggaggacag ctcgcagtag
    17161 agcagatttt cggctggaat acaacagtga gtgtcgtcgt tgaatttgtc ggaggaattt
    17221 attttgtcac aaaattgatt cgcgaaagga agagtatcct atgattgatt tacaaaaggt
    17281 caataaactg taccatggaa aatcagtggt taaagatgta cagttaacga ttcctgaaaa
    17341 gaagttgact gcttttatcg gtccaaatgg tgctggaaag tcaacggtgt tgtcgatgat
    17401 cagtcgtttg ataccaaaag atacaggtga aatctattta gaccataacg aagtaaaggc
    17461 ttggaaatcc aatgaattag ccaaaaaatt atcgatccta cgacaatcga atgaaataaa
    17521 tgtccaaatc accgtgaaag aattagtaga atttggacga tttccttata cgaaaggaaa
    17581 attgacgaca aaagacgagg aaatcgttca ggaatcactg gaacaccttg ggttagaaga
    17641 aatagcggat caatcgattg acactctttc aggcggtcaa ctccaacgtg cgtatattgc
    17701 aatggtgcta gcgcaagaca cggattatat tttactggat gaacctttga acaatctaga
    17761 tatgaatttt gcggttcaaa tcatgcagat tttaaaaaat ttagtacaag aaatcgggaa
    17821 aacaattgtt attgttcttc atgatatcaa ctttgctgca agttatgcag acgaaattgt
    17881 tgcaatgaaa gatggacagt tatttacgca aggaacgacc caagaaatga taaaaaaaga
    17941 tgtattggat gaattatacg aaatggacat acggatttgt gagattgaag gccgcagatt
    18001 ctgtctttat ttctaaaact aacattagga gagaagagat gaaaaaatta gtagtaggta
    18061 tcttgattct agcatcattt ggtttagcag catgtggaaa tagcaacaac accagtcagg
    18121 cagacacaaa aaaaagcagc acacaaactg aaactacatt gacgataaca gatagtaatg
    18181 gagaccaaat cgaggtgcca aataatccag aaaaagtcgt cgtattcgac aatggttctt
    18241 tagatacgat ggatgctctt ggtgttggag ataaggtagt tggagcagct acaagcagcc
    18301 ttcctgagta tctttcatcc tataaaaaag tagaatcagc aggtggaata aaagaaccag
    18361 acttagaaaa aattaatcag cttcagccag atctaatcat catttctggc cgacaaagag
    18421 attttcaaaa agatctaagt caaattgctc cgaccatttt cctttcttta gatgcgaaaa
    18481 atccatggga atctttccag caaaatgtca ctgctttagg ggagatcttt ggtaagcaag
    18541 aagaagctaa aactcaatta gaggaattat ctagtgcaat cgatcagacg aaaaagaaag
    18601 cagaagctac tgacaaaaaa gcactagtca cacttgtgaa tgaagggcaa ttatcagcct
    18661 acggatctgg ttctagattc ggtttcatcc atgatttatt tggctttgaa caagcagatg
    18721 atcaaatcga agcatccact cacggtcaaa gtgtttctta tgaatatgtg ctggaaaaaa
    18781 atccggatat cttatttgta gtagacagaa caaaagctat tggtggagac gacagcaaag
    18841 atgatatttc tgccaatgaa ctggtagcac aaacgaatgc tggtaaaaat cagcagatca
    18901 tctcccttga accagatgtc tggtatttaa gcgggggcgg tttagaatca atgaaactga
    18961 tgatcgaaga tgtgaatcaa gcatttaaat agcataaata acagaggatt aaactctcat
    19021 actatgtaac aataatgttt cttacattct taaatagcaa gagcctatcc tcaaagaagt
    19081 tatcaaaaac catccgtaaa atcgtattga tgattttacg gatggttttt atttatttga
    19141 gaaatctttt ttcaacgtga attcattatc tacatcatac gggttgattc tagaatgggg
    19201 agtggcaaaa aatagtgtga tccatttttt cagcatcaag atcgcctcct ttgatttgaa
    19261 tcaaaggtac aaagcgaaac ttagataaaa cttaaattta gatattttta ttttgtaccg
    19321 atctcttctt taaacgatgt tccatctgct gcttctcggc aataagtcca ctagactaga
    19381 aaatatgaga agctattttc tgtccagcgt tcttattact caaaacaaat cgcagttgtc
    19441 acaatctctt ctaaacgatg ttccatctgc tgcttctcgg caataagccc actagactag
    19501 aaaatatgag aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt
    19561 cacaatctct tctaaacaat gttcaatcag ctgaccttcg gtattagctc aaagcactga
    19621 tttcacaacc tctatttagg catgtgtctc agcaagcggt tgatagttga tgatctgcat
    19681 ttttcgataa aaatcggcgg gaagcttact ccaccgtttt agtaattgat agaaataact
    19741 ctcttctgct ttgattttat actcgcttaa tatcaatcca aaactattcg ttttttttga
    19801 agctaatttt ttataaatat ccaatgcttt ggttttatct tcattgacaa agagtgtttt
    19861 tacttcaata taagaaccct cgatattttg tgtttcttcg ataaaataga tgttttttat
    19921 atattccttc atagtgattc ctccaaagaa gtcttttatt tgataagttt actctaccga
    19981 agagttgtta ataatccgtg aacaaaaagt aattcttttt ccaatagttg ttaaaggttt
    20041 tttatttttt atagtatttt ttgttattta agaaaggaat aaggttaaac aaataacagg
    20101 ttgctttttg ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa agttgtctct
    20161 tttgtgataa gagaaatcat aataaacgaa tgacttactt tccaataaat tgagaacagt
    20221 cagaaccaga caaattagtt gcatctgatg aataaagtga ttattctatg acatgaggtg
    20281 atacagatga aattagaagt gacaaaagaa gcacaagaag tattgaagaa taaattagaa
    20341 gcagacgatc aacttttatt agatatcgaa aacggtgacg gtccatttgc agaaagccaa
    20401 gtcagttgcc aattggatac ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca
    20461 gatctaagtc tttattctgt agtggctgat acaccagttg gaccaatcaa aatcaaagac
    20521 agtgcaatac tttacatgga tgatccatcc acacttgctt tagaaccaac ttataatagt
    20581 ttacaattaa aaggaccaag cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac
    20641 tagagggata aaatgggaaa attggcgatc atcagcgacc tacatgtgga catcaataaa
    20701 ttcggcgaat cagagctgat gcagctttgg caagttttgc aaaagcacaa gatcactcgg
    20761 cttcatctag ccggagatac ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt
    20821 ttcgtcaata aaggattatc tacgacattt catttcggca atcacgagtt ggcagatgtg
    20881 cccaatgaac agatgatgga ccattttcca gacccacatt ttttaaatga acggtatatt
    20941 gctttaaatg agcagaccgt tcttttaggg atgaatggct ggtacgacta tcagttttcc
    21001 gaattgaaag attacgaggc aatcgtacga atgaagcgtt tgtattggta tgatcggttc
    21061 atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat
    21121 ttgctagacc agctggaagc aaaaaaactt gaggtgattt tatccactca tttcgttcct
    21181 aaacgggcat ttatcatgta tcaatctgct ccttatgagc gttggaacaa attaaatgca
    21241 tttttaggat cagaatcatt cggagaatta cttgaccgtt attcaaatat caaacaagta
    21301 gtttttggcc atacgcatcg tcgctttgaa gataaatcca tcggtcacac gatttacagc
    21361 tgcagacctt ttggttatta ttatgaatgg cagctgaccc gagattttgt cttgaaagaa
    21421 cagttgctag acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca
    21481 gcttttcagg cctatcaaac acaacatttg caagaggaat ttgaacaagc gatgacaatc
    21541 gttccgtatt gagacagcat tagtaatgtg taaaagaaaa taccttgtaa aacataagga
    21601 taagtaagag cagtagatcc atttatcgtg gatatgctgc tttttttgcg gaaaataatc
    21661 agcgaatgtt tatttgatcg gatgatttgt aactcgcaga gtactagatg caagcgtgta
    21721 ttcttttttt gaacgataat cacaaaaaaa aatataatat acatttttta tttaattata
    21781 attaatattg attgaagtct aggcagtcaa gtcacatttt gatagatagg agggggactt
    21841 acgaattatt ttaaaggcta tgacagctgt atttctagat tgtgtttgca tcaagttgca
    21901 ataaaaacta gaaatcaaat cgggctgata atcatttata actaatcgat acataggacc
    21961 aaatataggt ctatgttctt ttatttcata agtatcgtaa aacagaattt tgctatggat
    22021 taatccaatc gactgatatg tttagtaaat aaagaaagag gaatgtcaat gatgaaaaaa
    22081 gaaatcgtat taacagcagc aatgatgtta ttcagcatgg tagcaagtac gacttttgtt
    22141 tctgcaactg aagtataccc aaaggaatat aacacagaag gaacgattac atttgaagca
    22201 ggagatgagg gtgtaactcc tcctgttgat ccagagaatc ctgatcccaa taaacctgtc
    22261 gatccatcag atcctccaag ccctggaaca ggaggggcat tatcgattga ttatggatca
    22321 aaattcaaat ttgggactca aaaaatttct actgcggata aaacgtatta tgcagctgct
    22381 gatgtgatga acgatgggtc tagaaaaccg acatatgtcc aagtgacaga taggagaagt
    22441 accttatctg ggtggaaatt aagtgtctct caaccagaac agtttaaaac tgccagtggg
    22501 gatgaactag ttggagcaca gctgaaattt acgaaagggc aggctgtttc tttggttgat
    22561 ccaacatata cgccgcaaac ggtgaattct gaattaacgt tgacaccggg aggaaacaat
    22621 acgttagcaa ttaatgctaa atcaggaaca ggtgtgggga cttgggtcta tcgatttggt
    22681 gcaaatgaaa atgaaaatca agatgcggta cagctttcgg tacccggtaa atctgtcaag
    22741 ttagcgcaac aatattccac taaattagtt tggacattag aagatacacc taacaactag
    22801 tttactgaaa agaactgtta aaaataaata cgtagtcata ttgtcttgcg aggtaatcga
    22861 gcaaaaattg tcaaaggcta ggctaaattt gtttggatac gaatagttca tctaggaatt
    22921 taacgatcag gaggaaaaga tatattgaaa aagaggagga tcgtgctcat tttaagcagt
    22981 ctcttagtga ctaatatctt tccgatcata gggtactctt tggaattgaa tcagttggag
    23041 catggaaata gaagagacga agaaagcaaa ctcacttcga cattcgacag tttaatagaa
    23101 tccaaccaga cacaagaaag cagtgaggat ttaagtattt cgagtagtgg agagaccact
    23161 gacaacgtag aagctgaggg ttcggacaaa caaggactag aagatgcacc aatttctgcg
    23221 gttgagctgc ctgacgatcc caatattatt tctatcgata aaatttttca aacacctatc
    23281 ggtactagca cgtccatatt ggaaaatggg aagttactgc agttgaaccc agcagaaagg
    23341 tctcaaagag gagctatttg gtcaaataag aagattagtt tactctcgga ttttacattt
    23401 aaatcttatt tatacttagg aaatcaacga gatagagcgg gagatggcat gacctttaca
    23461 ttgactggcg atcctagaat ggctagtaat cccagtcaag ttattggatc gccaggtatg
    23521 ggaattggct catattctac tcgttctggc caaccttaca ttgcgaatgc gctctcgatt
    23581 gagtttgata cgtataaaaa tagtggttct tctgatcgaa tggatcgaga gataactgct
    23641 gataatagag ggcatggaca tgtcgctttt gtcacgccta aaccaaataa taataattat
    23701 tctggagagc atactggcgt gacggtagct cctacatttc tttctaatgg cacatggcgc
    23761 ttgttaataa ttaaatggga cgcggagcaa agaagtctta cttatgattt ggaaggtgtc
    23821 ggtcgctctt ctcggattat acctgatctc attaggcagt ttggttctac agaagtaaat
    23881 tgggggttta cttcttcaac aggtactttt tttcaagaaa atgctgtcgc tgtcacgcga
    23941 cttccatcga atgtcacatc aacagctgat gtaaaagtca acgatagtga ttatggggcc
    24001 atcgctgaag ctggaggaaa cgataagata actatcagaa atacattgaa ggtcgagggg
    24061 gattttcttg aatataagaa aatgcagact agtatcgacc tcccacaaga attaactatt
    24121 cccgagcata tcttgatcaa tggaaatcaa attgatgcca aagatatctt gataaccgga
    24181 aatcgagtca cgctaaatct gacagattat tttattgctg gagatacgtt aataatcgaa
    24241 ttggagacaa ctttgcagga agtaattcct gaaaaagtac tcactatgca ttttgaatac
    24301 cttgaaaacg ggaatttgat tgagcaatcg aatgaaataa acctaaagat cgcaaagctg
    24361 aaggagatag atatccatgt ttattataaa gataaagaca gtcagattga tattgctgaa
    24421 atgaaaacaa tcactgggaa aataggagag aagtataaag aaactcctat tgatattgaa
    24481 gggtatgttt ttgactcgga ttcagcgaat acagaaggca tcttctccga aaatacagag
    24541 gatatctatt tttattacag aaaaggcaaa ctttatctct caactgctcc tcaatatgtc
    24601 aattttggaa aacataaaat atccagtaga ccattaagtg aattcggaca gttttcaggt
    24661 gggttgaaaa ttatagatga acgggctttt ggaagttgga aattacaatt gaaacaagag
    24721 aaagttctta caaatggaga ttttgagctt ccagaagctc tttcctttgt tacctctgaa
    24781 aatacaacgg ttatagggac atccgctgtt acgattttcg aaagtgatca aaagggtgaa
    24841 agcgatttgt cctctctttt agattctaca gctcatcgtg gaatcagaat aaatattcct
    24901 gtagaaaacc aaagaatcgg aacctttgag gggaaactat cttggatact tgcagatgtc
    24961 ccaggaaatt aacaggaaga gaagaatata acaaaattat tgagtttcaa gaagggcaca
    25021 ggtaccatag gctcttgtgt cccacagaga caagattttg ttatagtctt ctcttacata
    25081 tgattaaagg agcgattttc ttgagaccaa aacaacaatt atttataaca ctcctagtta
    25141 ttttaggggc actttttaac aatatatcag tgtcagctag tgcatttaat ttttcagtga
    25201 cgccgataac ttcagagaat caaatcgaca agagaaaaac ttacttcgat cttcaacttg
    25261 atccggatca ggaagtagaa gtaaaagcgg aattgagaaa tgatacagaa aaagaagtga
    25321 aaatcgatat ttctgtgaat agcgcaacca ccaattcaaa tgtgatggtt gaatacggaa
    25381 aaaacgagat tgaaaaagac atgagcctga tctttgatct ggtagattat gtgagctatc
    25441 cacagatagt gacgctaaaa cctaaaagtg tccaaaccgt tgcttttcat gtcaggatgc
    25501 cgaatgagcg atttgatggt gtattggctg gtgggatcac attccaagaa atccagacgg
    25561 aaaaggacca aacagaaact aaggatcaaa gcttatcgat tgagaatgaa tacacctata
    25621 ttattgcttt gttgatgcaa cagacactga acgaagttgc tccgaacctt actcttcatg
    25681 aagttaaacc agatcaaatc aatgcaagaa atgtcatctt agcaaacgtt caaaatgatc
    25741 aaaaaaccta tatcaatcaa gtagttattg aaacaaaaat tacaaaaaaa ggacattctg
    25801 aagttttata tcaagaggaa aaagaaggat tgcagattgc gccgaataca aatttttctt
    25861 ttccaactgc tttaaatggt caaccgctaa ccccaggtga atatcacctt acaatgactg
    25921 ttttaggaaa tgaaaatgaa aatggaaaat tttcgagaaa aaaaggaaat actacgatca
    25981 actataccaa tcaatgggta tttgaaaaag attttacaat agacggaaaa gtagccaagg
    26041 aattgaacac gacggatgtt acgattaaaa aagacaataa ttggatttat atcttagtag
    26101 cttcactttt cttattgttt attttattat gcatactttt gtttgttttg agaaaaaaag
    26161 aagagaaaga aaagtaaggt taagataa
//
LOCUS       DAZUMI010000028        26165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000028 DAZUMI010000000
VERSION     DAZUMI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.028"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG81C_001015"
     CDS             complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG81C_001015"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5064193.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1290..2465
                     /locus_tag="ACG81C_001016"
     CDS             1290..2465
                     /locus_tag="ACG81C_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5064194.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2469..3110
                     /locus_tag="ACG81C_001017"
     CDS             2469..3110
                     /locus_tag="ACG81C_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064195.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3107..4024
                     /locus_tag="ACG81C_001018"
     CDS             3107..4024
                     /locus_tag="ACG81C_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5064196.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4021..4692
                     /locus_tag="ACG81C_001019"
     CDS             4021..4692
                     /locus_tag="ACG81C_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064197.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5233..5721)
                     /locus_tag="ACG81C_001020"
     CDS             complement(5233..5721)
                     /locus_tag="ACG81C_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5064198.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5922..6188)
                     /locus_tag="ACG81C_001021"
     CDS             complement(5922..6188)
                     /locus_tag="ACG81C_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5064199.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6188..6451)
                     /locus_tag="ACG81C_001022"
     CDS             complement(6188..6451)
                     /locus_tag="ACG81C_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5064200.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6972..7112
                     /locus_tag="ACG81C_001023"
                     /pseudo
     CDS             6972..7112
                     /locus_tag="ACG81C_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7254..9077)
                     /locus_tag="ACG81C_001024"
     CDS             complement(7254..9077)
                     /locus_tag="ACG81C_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5064201.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9675..12380)
                     /locus_tag="ACG81C_001025"
     CDS             complement(9675..12380)
                     /locus_tag="ACG81C_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5064202.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12686..13633)
                     /locus_tag="ACG81C_001026"
     CDS             complement(12686..13633)
                     /locus_tag="ACG81C_001026"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5064203.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13823..14092
                     /gene="rpsN"
                     /locus_tag="ACG81C_001027"
     CDS             13823..14092
                     /gene="rpsN"
                     /locus_tag="ACG81C_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5064204.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14268..14630)
                     /locus_tag="ACG81C_001028"
     CDS             complement(14268..14630)
                     /locus_tag="ACG81C_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5064205.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14641..15762)
                     /gene="alr"
                     /locus_tag="ACG81C_001029"
     CDS             complement(14641..15762)
                     /gene="alr"
                     /locus_tag="ACG81C_001029"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5064206.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15778..16128)
                     /gene="acpS"
                     /locus_tag="ACG81C_001030"
     CDS             complement(15778..16128)
                     /gene="acpS"
                     /locus_tag="ACG81C_001030"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5064207.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16258..17769)
                     /locus_tag="ACG81C_001031"
     CDS             complement(16258..17769)
                     /locus_tag="ACG81C_001031"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5064208.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18091..19443)
                     /gene="murF"
                     /locus_tag="ACG81C_001032"
     CDS             complement(18091..19443)
                     /gene="murF"
                     /locus_tag="ACG81C_001032"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5064209.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19596..20672)
                     /locus_tag="ACG81C_001033"
     CDS             complement(19596..20672)
                     /locus_tag="ACG81C_001033"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5064210.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20868..22613
                     /locus_tag="ACG81C_001034"
     CDS             20868..22613
                     /locus_tag="ACG81C_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5064211.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22893..23330)
                     /locus_tag="ACG81C_001035"
     CDS             complement(22893..23330)
                     /locus_tag="ACG81C_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5064212.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23314..24687)
                     /locus_tag="ACG81C_001036"
     CDS             complement(23314..24687)
                     /locus_tag="ACG81C_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064213.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25009..25497)
                     /locus_tag="ACG81C_001037"
     CDS             complement(25009..25497)
                     /locus_tag="ACG81C_001037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064214.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25519..26121)
                     /locus_tag="ACG81C_001038"
     CDS             complement(25519..26121)
                     /locus_tag="ACG81C_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5064215.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7504 a         5561 c         4837 g         8263 t
ORIGIN      
        1 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
       61 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
      121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
      181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
      241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
      301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
      361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
      421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
      481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
      541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
      601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
      661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
      721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
      781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
      841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
      901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
      961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     1021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     1081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     1141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     1201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     1261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     1321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     1381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     1441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     1501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     1561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     1621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     1681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     1741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     1801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     1861 ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg
     1921 aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt
     1981 tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag
     2041 tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag
     2101 caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg
     2161 tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg
     2221 tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata
     2281 ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca
     2341 aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg
     2401 gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg
     2461 tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg
     2521 aacatcttta tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg
     2581 gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct
     2641 tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta
     2701 aattacctgc gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt
     2761 acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga
     2821 ctgagttcca atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg
     2881 gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg
     2941 ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt
     3001 tggcaggtac gatccctgta acaattttag cactattggc tgattggctt ctaggtctct
     3061 tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact
     3121 aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct
     3181 tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca
     3241 gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat
     3301 catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc
     3361 aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga
     3421 aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca
     3481 aacgtggttc ccgtcctatg gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac
     3541 cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt
     3601 agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga
     3661 aacttatggc ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc
     3721 agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag
     3781 ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc
     3841 agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt
     3901 aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct
     3961 agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa
     4021 gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat
     4081 ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc
     4141 tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca
     4201 aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc
     4261 attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat
     4321 tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc
     4381 cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta
     4441 ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg
     4501 ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga
     4561 accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg
     4621 gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt
     4681 acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa
     4741 ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg
     4801 ggcaaagagt tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc
     4861 aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc
     4921 caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc
     4981 tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc
     5041 cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg
     5101 ccgtatcggt aacttcgatc atcttttata caatcaggaa cttgccatcc tcatccttgg
     5161 ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc
     5221 ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt
     5281 caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt
     5341 ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga
     5401 aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt
     5461 ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc
     5521 aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat
     5581 tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc
     5641 ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat
     5701 tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc
     5761 cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa
     5821 tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga
     5881 gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag
     5941 gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt
     6001 ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc
     6061 gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa
     6121 ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag
     6181 ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt
     6241 tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa
     6301 tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa
     6361 tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag
     6421 cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta
     6481 tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt
     6541 ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca
     6601 cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca
     6661 tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag
     6721 gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag
     6781 atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg
     6841 attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg
     6901 ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac
     6961 ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg
     7021 aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc
     7081 aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc
     7141 tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga caaaacagac
     7201 cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc
     7261 ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt
     7321 aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga
     7381 ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca
     7441 taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca
     7501 tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct
     7561 agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg
     7621 atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt
     7681 agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc
     7741 ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac
     7801 cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca
     7861 atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga
     7921 gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag
     7981 ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa
     8041 tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga
     8101 ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc
     8161 gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt
     8221 tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg
     8281 atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt
     8341 tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa
     8401 gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc
     8461 gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca
     8521 acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat
     8581 gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa
     8641 atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct
     8701 gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag
     8761 ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa
     8821 gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg
     8881 gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt
     8941 tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca
     9001 aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac
     9061 ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag
     9121 tcgaaaattt atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa
     9181 ctttaagaaa ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa
     9241 tacagcaaaa atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat
     9301 agaacttgac ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag
     9361 atgtaaggct gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa
     9421 cctcttgata aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa
     9481 aacataagaa gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta
     9541 caacctcttg gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc
     9601 aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt
     9661 cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc
     9721 attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa
     9781 ccgatcatga tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc
     9841 cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc
     9901 cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata
     9961 atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg
    10021 aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact
    10081 cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa
    10141 aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt
    10201 cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat
    10261 agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg
    10321 gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat
    10381 aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct
    10441 ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc
    10501 acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca
    10561 ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga
    10621 atgatcggta attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta
    10681 tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt
    10741 tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg
    10801 agaagcgaat caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc
    10861 gtatcgatga cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata
    10921 ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag caattcatca
    10981 atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga
    11041 ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt
    11101 ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt
    11161 tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca
    11221 gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt
    11281 gccacttgtc caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca
    11341 tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct
    11401 aaatttttca catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt
    11461 gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct
    11521 gctagttgct taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt
    11581 tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc
    11641 tcatctactt tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt
    11701 tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa
    11761 ttttcatctg ttgataaaat catatcttta actttattga tacttactag gttcgtatcg
    11821 atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat
    11881 tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc
    11941 gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta
    12001 aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg
    12061 gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta
    12121 tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct
    12181 gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc
    12241 cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc
    12301 agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc
    12361 gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac
    12421 catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat
    12481 cacggcaatt gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct
    12541 ttactttatc atgaaataaa agggaaagat aattttatgc caaagttttt atctattata
    12601 cgtatacgca aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt
    12661 ttttcgtttt ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag
    12721 gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat
    12781 tcattgaaat aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg
    12841 cttctgcatc tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt
    12901 gccgcaaatc tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga
    12961 tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct
    13021 gatcagcaaa ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta
    13081 atttttttac cttttcctca tttttactgg atgcactggt taagattcgg attccctttt
    13141 catggagaag ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag
    13201 tatctgtcgg ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg
    13261 tgaccattcc ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg
    13321 atggcaacac gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac
    13381 ctacatgaac agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac
    13441 ctggtacata agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa
    13501 catcataagg attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt
    13561 ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg
    13621 caaatcttcg catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt
    13681 tgacaaaaaa aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt
    13741 tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa
    13801 aaataattgg aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca
    13861 gcaagaaacg attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta
    13921 cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt
    13981 aatcgatgga cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg
    14041 tgaattagca cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc
    14101 tggctgtaat gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac
    14161 gttcgcttct tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca
    14221 gctggtcctt ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca
    14281 ggagtcagtg cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg
    14341 ggtcgatcca aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc
    14401 tccaacagga taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga
    14461 gtcggaagct taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta
    14521 aaatggttac ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat
    14581 cccacgactg gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc
    14641 ttattcttga tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat
    14701 cgtacctaat tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat
    14761 aagcgtcact tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat
    14821 acatacacgc ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat
    14881 ctttcgtggc cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg
    14941 agtgatatat gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat
    15001 caattcagaa accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc
    15061 agagggatta agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc
    15121 atgccataaa gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt
    15181 tttcagcact tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc
    15241 tgctgtagaa aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa
    15301 tgcagctgcc tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc
    15361 tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc
    15421 tgcttgcaac cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt
    15481 cagtgcgtcg taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct
    15541 caactcgact ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc
    15601 tgcctcagct gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa
    15661 ttcttttcct tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt
    15721 atgtatcact gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta
    15781 gtcttccaaa ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac
    15841 attgccttca tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc
    15901 gacatcttga aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt
    15961 gcaagcgtaa cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag
    16021 tttcatttca tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg
    16081 agtgatccta ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct
    16141 tttcaataaa tttagaacac tctcattcta ccaaattata accattttgc cacaaataag
    16201 tccacaaata acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta
    16261 atcgttattt gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc
    16321 tttttggctg ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc
    16381 gccgtcacga cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt
    16441 tttctgcatt ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc
    16501 ttttgctgtc gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa
    16561 tttatctgct gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg
    16621 ttcaactgca gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt
    16681 cgtcatccgt tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt
    16741 aacgaaagtc acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac
    16801 gtagctttct ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc
    16861 taatccacgt gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga
    16921 acgcaatacg ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta
    16981 accgcgtgct tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac
    17041 gattgttaat tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta
    17101 ttcttttgcg cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat
    17161 cttcacatga tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat
    17221 cgttgctgag aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat
    17281 atcttccaag aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac
    17341 ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac
    17401 aacgatatgt ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta
    17461 aacggcttgt acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg
    17521 gatagctaat tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc
    17581 gattttttct aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg
    17641 tccaataacg tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc
    17701 ttcttcaaaa ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt
    17761 aaatttcaaa tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc
    17821 cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta
    17881 tccattctta gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa
    17941 aagccaaacg ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt
    18001 ttctatggca aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata
    18061 gtttttcgtt ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac
    18121 ttcaaataag cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt
    18181 ttgtttgata gcatcaatca atttattctt ttctttttcc aaatagaaca caggtagttg
    18241 ggggtatttt tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat
    18301 catggcgaat ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc
    18361 tttccctaat tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt
    18421 tttaaaagta tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa
    18481 aatagctgca ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg
    18541 ggcgagccct tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc
    18601 aatcaaagca ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc
    18661 tactacaaaa tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt
    18721 tccaataccg aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg
    18781 ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt
    18841 ggctttagcg attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt
    18901 gattgctgca gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc
    18961 catgcccatt tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg
    19021 aagaccaata ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa
    19081 gacagacgcg gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac
    19141 ttctgcgcct actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc
    19201 tgctacagga ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga
    19261 agcagatgct ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc
    19321 aagaggaacg aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat
    19381 ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt
    19441 cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg
    19501 catgactctc acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg
    19561 ttacgcaatc actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat
    19621 tgacgctgat ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat
    19681 ttcaagccca tattttccca taagagtggg tacatactga actccgtaaa tcctggcata
    19741 gagtttaatt cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc
    19801 aatccgcttc cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa
    19861 acttcttctg gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct
    19921 tcataatcat agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct
    19981 tcatttccta atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct
    20041 cgagaatcat actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct
    20101 tttgtaatgc cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa
    20161 gaaccttcac attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact
    20221 ggtacataag gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta
    20281 tccatggcac atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct
    20341 aagaagcctt ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg
    20401 gctccttctt ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc
    20461 tgtccacttg ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt
    20521 aatggacctt tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa
    20581 ttataataaa tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg
    20641 ctgcgtccgc catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt
    20701 tcagtgattt actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa
    20761 aaaaacgagc cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt
    20821 gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa
    20881 atccacgcag ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa
    20941 actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca
    21001 ccttcatcaa gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca
    21061 ctcaaacatt caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa
    21121 agacgatcga aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat
    21181 cgccttttgc ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg
    21241 cggtagtcgt taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac
    21301 tatttgaacg agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa
    21361 aaggggtact ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa
    21421 gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca
    21481 agatttatga cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa
    21541 acttgaatca tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa
    21601 acaagcagct gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt
    21661 ttgatgatgg ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa
    21721 caaaagaaga acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg
    21781 gttcctttgc ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca
    21841 actacatcga ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac
    21901 aagccgtttt agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac
    21961 tttgtcagca agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc
    22021 ttgaacagta ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg
    22081 tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga
    22141 aagcatttgg gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg
    22201 aagtcgcttc gccttttgct caacattatt ttctcactca tctggatgta gaagatacat
    22261 tgaaacaaag cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac
    22321 agctccacca tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga
    22381 aaaagcaaga caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta
    22441 tgattttagc tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa
    22501 acgcagaggt cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta
    22561 tttttttggc aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg
    22621 ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca
    22681 aatttttgtg aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa
    22741 aaaatgtgaa aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa
    22801 cacatttatc ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt
    22861 cgttacctac tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga
    22921 cgaaaagaca tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct
    22981 tttcttaact ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca
    23041 aattcttttc cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa
    23101 ccataatctt gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc
    23161 gcactatcag ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc
    23221 gtataatggt ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt
    23281 atgacatctt cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc
    23341 cttcttccat aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca
    23401 ttttgccaag ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat
    23461 caataataga tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa
    23521 taaaaactgg tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg
    23581 atccattgga atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac
    23641 tacgtgccag aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac
    23701 caataaagaa gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt
    23761 caaaatccat ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat
    23821 aaaaatcgaa agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa
    23881 aaggaaattc cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt
    23941 ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc
    24001 ataaatagca agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa
    24061 caataagcca aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt
    24121 gatgtctttg aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac
    24181 gtaaaaaagt gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat
    24241 cgcttgatac aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt
    24301 gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc
    24361 tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa
    24421 tattccaagc aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact
    24481 atctagacca aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa
    24541 cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag
    24601 ataaggaata aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg
    24661 cccaaatcgt ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat
    24721 ctgctcttcg attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt
    24781 aaaaaaaaat acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact
    24841 caagcagaca agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga
    24901 taaagataga aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga
    24961 gtccttctcg acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc
    25021 ttttttcatt ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc
    25081 tgtaatctca aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac
    25141 tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac
    25201 tgaagaagtc aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat
    25261 ttttccattt atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa
    25321 aaagtaggac acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt
    25381 cagcatgtaa tccacttgtt tttgaccaat gatcggcaca aaagcttccg gccatacttc
    25441 ttgtatgact gccatcagct catccagttc attttctgtt acttttattt tttccataca
    25501 cccacgtcct tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat
    25561 catgctatac acacccaccg accatggttt caagaaaatc acaatctgca taaattccct
    25621 aaaagataat ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag
    25681 cagcaaggta acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc
    25741 aaatttctga tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg
    25801 gacaagcaaa aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc
    25861 aaatccattg ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg
    25921 aatgatcact tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg
    25981 ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag
    26041 aatcaacaca aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa
    26101 aactgatcct tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc
    26161 tcctt
//
LOCUS       DAZUMI010000029        25659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000029 DAZUMI010000000
VERSION     DAZUMI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.029"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG81C_001039"
     CDS             complement(<1..132)
                     /locus_tag="ACG81C_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5064216.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="ACG81C_001040"
     CDS             542..781
                     /locus_tag="ACG81C_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064217.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="ACG81C_001041"
     CDS             848..1057
                     /locus_tag="ACG81C_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064218.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="ACG81C_001042"
     CDS             1117..1788
                     /locus_tag="ACG81C_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5064219.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVLAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="ACG81C_001043"
     CDS             1841..3817
                     /locus_tag="ACG81C_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5064220.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3832..4584
                     /locus_tag="ACG81C_001044"
     CDS             3832..4584
                     /locus_tag="ACG81C_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5064221.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4600..5355
                     /locus_tag="ACG81C_001045"
     CDS             4600..5355
                     /locus_tag="ACG81C_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5064222.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5368..5628
                     /locus_tag="ACG81C_001046"
     CDS             5368..5628
                     /locus_tag="ACG81C_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064223.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5625..7715
                     /locus_tag="ACG81C_001047"
     CDS             5625..7715
                     /locus_tag="ACG81C_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS5064224.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7742..8206
                     /locus_tag="ACG81C_001048"
     CDS             7742..8206
                     /locus_tag="ACG81C_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064225.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8203..8445
                     /locus_tag="ACG81C_001049"
     CDS             8203..8445
                     /locus_tag="ACG81C_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS5064226.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8463..8765
                     /locus_tag="ACG81C_001050"
     CDS             8463..8765
                     /locus_tag="ACG81C_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064227.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8835..10898
                     /locus_tag="ACG81C_001051"
     CDS             8835..10898
                     /locus_tag="ACG81C_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064228.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10898..13510
                     /locus_tag="ACG81C_001052"
     CDS             10898..13510
                     /locus_tag="ACG81C_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5064229.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13507..16038
                     /locus_tag="ACG81C_001053"
     CDS             13507..16038
                     /locus_tag="ACG81C_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS5064230.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            16088..16420
                     /locus_tag="ACG81C_001054"
     CDS             16088..16420
                     /locus_tag="ACG81C_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064231.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16421..17014
                     /locus_tag="ACG81C_001055"
     CDS             16421..17014
                     /locus_tag="ACG81C_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064232.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17177..19201
                     /locus_tag="ACG81C_001056"
     CDS             17177..19201
                     /locus_tag="ACG81C_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFS5064233.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19201..20400
                     /locus_tag="ACG81C_001057"
     CDS             19201..20400
                     /locus_tag="ACG81C_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5064234.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20416..21060
                     /locus_tag="ACG81C_001058"
     CDS             20416..21060
                     /locus_tag="ACG81C_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064235.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            21071..21877
                     /locus_tag="ACG81C_001059"
     CDS             21071..21877
                     /locus_tag="ACG81C_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064236.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21899..22129
                     /locus_tag="ACG81C_001060"
     CDS             21899..22129
                     /locus_tag="ACG81C_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064237.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22643..22951
                     /locus_tag="ACG81C_001061"
     CDS             22643..22951
                     /locus_tag="ACG81C_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064238.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22953..23375
                     /locus_tag="ACG81C_001062"
     CDS             22953..23375
                     /locus_tag="ACG81C_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064239.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23395..24984
                     /gene="mobP2"
                     /locus_tag="ACG81C_001063"
     CDS             23395..24984
                     /gene="mobP2"
                     /locus_tag="ACG81C_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS5064240.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24996..25331
                     /locus_tag="ACG81C_001064"
     CDS             24996..25331
                     /locus_tag="ACG81C_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064241.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25472..>25659
                     /locus_tag="ACG81C_001065"
     CDS             25472..>25659
                     /locus_tag="ACG81C_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064242.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9580 a         3999 c         5253 g         6827 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt acttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcagaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctga gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtctat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgctta ttggaatgtt aggaatcatt gcaggctgct ttcagggata
     1921 tcgagcatat gcagaagata tcacgcaaaa aacgccacca gaaaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga ctgaagattc
     2161 tgatctaacc aacgcgaaag tggacgaaat cgttaaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caacaaacaa aattacttca gctgtagatg aaaatggtga
     2281 gattactttt gctgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgact gataatacag gttcaggttt tttaaaagac attcacttat atcctaaaaa
     2461 tattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggca cagctcaaag
     2521 caaacaaaca cctttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaatta ggagatcttg tatcggatga tcaaggaaag ctgactgcta ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcagaagtt gtagtgggat cagataaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac aattcatatc ccaacagaca ttgcaggcgg
     2941 agcagacggt atcacagtaa acggagtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggggat acagcaggac aaggactttc ttatgtcgct gctaaagcaa atatcaaagt
     3061 aacaaataaa gatggatctg tggtattaaa agaaaataca gattataaaa tccaaaattc
     3121 agaaaatggc tttgtgatcg attttatcgt gaataatggc caagtaagtg atactgtagc
     3181 aagtttacac ggacaagatc ttcaaatgac ttataacatg tatgtaaatg acagcgcagc
     3241 tgttgccaat ccattaacaa atagcgtgga tttcgtttat aacaacaatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggtgcaa aattcttaaa
     3361 agtcgatagc ggactatttg gaacaggaat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgctgctaaa aacgcagaag gaaaatatta tggtggatta gttgatacgg ataaagacgg
     3481 tgtcaaagaa gcagtatggg tagacgatgt agcaaatgca gcgatcctaa aatcagataa
     3541 agaaggtcac tttgaaatca ctggtttaac tgaaggtgaa tattcactgg aagaaacaaa
     3601 agcgcctgaa aactatcaaa aattaacgaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaacaatcaa aaagcttccg taccaatgac
     3721 cggtagcaac ggttttcaaa cgtatgtatt aatctcttgc ttgttgctag gagcaggggc
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcaaaacc aatgattatc
     3841 aggaagccta agaaaaagcg aatcatgggt aaatggatca ttgctttctg gttactttca
     3901 gctgtaggtg tcttactttt gatgcctgct gaagcgagcg tggcgaaata ccaacaaaat
     3961 cagcagattg ccgcaatcga tcgaacagga acggcagcag aaacggattc ttctttggat
     4021 gttgctaaga ttgagttagg cgatcctgtt ggtattctta caattctaag tatttcttta
     4081 aaactaccaa tttatgacgg aacgtccgac aaaattttag aaaatggtgt aggtattacg
     4141 gaaggaactg gtgatattac aggaggaaac ggtaaaaatc ctttaatcgc aggtcatagt
     4201 ggactttata aagataatct ttttgatgat ttaccttcag taaaaaaggg agaaaagttt
     4261 tatatcaaag ttgatggtga gcagcacgct tatcaaatcg atcggattga ggaagtgcaa
     4321 aaagacgaac tacaaagaaa ctttgtcact tacttagaat caaatcccaa tgaagatcga
     4381 gtaacgctaa tgacatgtac gcctaagggg atcaacacac accgttttct agtgtatggg
     4441 aagcgagtaa cctttacaaa atcagaatta aaagatgaag aaaataagaa acaaaaatta
     4501 tcttggaaat ggcttttagg aagtactgtt tttctttcag tcatgatcat tggttcgcta
     4561 tttgtttata aaaagaaaaa ataaaggagg gtaaccaaca tgagaaatag aaaatggcta
     4621 tttgtttgtg cagccgctct tttttctttc actgcctaca cacagttgac gtttgcagac
     4681 agacaagaaa gcacggatag cacagaacaa acaatccaaa cgtcagatag cagcacgatt
     4741 caaagcagcg atgaaaccgt cgattcgtca acagttgatt cttcagaaga acctaaaatt
     4801 gctgaagacg atcaaatagt caaacagacc attcacgttc aaaagattat ttcttcagaa
     4861 caattagaca accaggggca tgaaaaacaa gaaggagtaa atggcgcgaa gttttctgtt
     4921 tatgatgtat cggacatttt acaaaagatg gatgttaagg atctcactac tgaccaaatt
     4981 gaatcccaat taaaaaatcg agtgaaaaaa ctatccagtg atcaattgaa gctagtttct
     5041 aatgaagaaa caaagacaat tgatcaacaa acaggtgtct ttgaattctc ggtagaagta
     5101 caggcaaatc agaaacaagc ttattatatt gtcaatgaat caagtcctga aaacatttct
     5161 aatagcgagg atattttgct attaacacct gtttctgata aaaacggtga attcttaaag
     5221 gatgtgtgga tctatcctaa atcagaagct tcgcaaccta aagaagaagt aaaaaaaatc
     5281 gtttctactg gcgtaaagaa aaacttcttt gagaattgtt gggattttgt tactcacttg
     5341 ttttctagag attaaggaag aaataaaatg aaactgatca aattattatt attcaatttt
     5401 tcttgtttaa ccgcagtctt tttgtactgc ggtttattat tttctaaact atctattttg
     5461 ccaagctacc aactatacct aatgaggatt ttaaacatac cagctgcttc catgccgcta
     5521 gatattatgg aaaagctcat taggaatcca ttatttacta cgatcaattt gatcgttatt
     5581 gtcggaattt cactagtcat ttataaaaaa gaggggatga agggatgaat tttaaacact
     5641 cgtggacaag acggttaaaa tatgtttttc ccataatgat gatgttaggt acattgtttg
     5701 ggttaaaaac agccaatgtc tttgctgaaa aagttattgt cgatccgtca aatccaatcc
     5761 agaacgtaaa gaataatttt attattccaa aagatgttcc taatgcaaaa acaaatatta
     5821 ctgtaaacgg agcgtcaaaa gtttacaaat atcatgttga tgccaacaaa ggtggtttta
     5881 cagacgacta cgtaggatta gtggagggaa acaaaaatat acgctatcct tctttccaaa
     5941 aagaatacgg tgaaacaaac cttgttttag aaggcgtaac aaccaacacc aaagtcaata
     6001 ttgactatgg aaaaatcggt acgtataacg ggaaaaaagt caatatcaag ctagttctat
     6061 ctaatattca cttatactcg gatacgttgc cgtggaatct actagataac aactatacaa
     6121 aaactcattt tcgagacgat ggctataaaa atacgaatgg tgcaatgagt aaatctaaaa
     6181 aaagaacagt actttggatc tcagacaatc ttttctcagg aattgtttac cattcaacac
     6241 aaatgaatgt gcagctagta gccacatatg aggacggtag ccccgttcaa ttttcaggag
     6301 atacatttat cagttttaac tctttgaacc ctgcaggagg aaaaagcacg gatcttaaag
     6361 gagaatatgc ccactatgac aaaatgaata cgacagattg gtatgtcaga aaagatacgg
     6421 ttctttcaga atttaaatct ttctacaaca atctgaacgt ggtaggtggc cacccaggag
     6481 gaagctctaa gctgacacaa gcggataatg atttcaataa tttgcatgat aaattaggag
     6541 atccgaagtt tggtcaaggt actgtgtcct ttaaaatatc tgaagcaaat ccgacgtttg
     6601 tgattggttc ttctaatgtt caaacctggt ttactttatc ttctgcgaca atctttagtg
     6661 ttgttcctga tcaacctgaa aaaacagggg ttgataaaaa tggaaataac gtgaacgata
     6721 aaatgttgca agtaggagac acgattcaat atcgaatcaa acagaaggtg aatcgattag
     6781 gagtggattt actagcggtt tacgatcgat ttgagttaat cgataattta ccaaaagaag
     6841 taaattacgt cgatgcacac gttgaaagtg gtacaaataa aaaattcgat gtatctggtg
     6901 aagtgaccta tgacaaaaca aaacaccagg taaaatatgc agcgaaagct gatacattaa
     6961 aaaatcgaat gaagtataac ggcgaaactt acgaattagt gatcaatgta aaagtcaatg
     7021 aattggccaa tcaaaatagt gtagccaaaa atcagggaac gtcgatcatt aataaagtag
     7081 aaaaggatac aaatatcatt accgtttatt tccctaaaat tccagtgaaa gaagtacaac
     7141 aaaatggaaa agatgtaaac ggacgaaatg acggtaagaa gggtacgccg acagctcctt
     7201 taaacgcagg atctgaagta caatatctag tcacacaaaa atggcacaca aaaggcgtag
     7261 acgctgcgtc agatcactac aaacaattta gtattcaaga tccaattgaa gctcgtttga
     7321 catataaaga aggttctgcg caagtaatag ataagagtaa aggaaaagat attacttctg
     7381 aaggcacact aacctatgat agcaattcaa gaacgctgaa gtgggaagct tccgctgact
     7441 tcttaagtaa caatctttta gatggacgag aaattcagct gatatttaca gctaaaactc
     7501 cattacaatc agaaaaaaat attgataacc aagccgtagt agcagtagat aatgtttcta
     7561 ataaaacaaa tgttgtgaca attggggttg atcctaactt gccacaggtc attgttccta
     7621 aaacaggttc tacacattta gtaacgattt cagcggtcag cttactatta cttgtgttag
     7681 caacttttag ttatgcggtt ctgagataca tttaaaataa aaaaacgaaa gaatgaataa
     7741 aatgaaaaag ttaataatag tagttacttc cttgattgcc atgattgtta ttttagtcgg
     7801 atgtagtaat gaaaaaaata aacaaactaa tcaagataat ggcgtattga aatcaggaac
     7861 tatgtggaaa gaagaagtag gtggtcttgt ttataatttg aaaattatag acgagactac
     7921 ctgggaatac tctgagagtg tatggcatcc agatcctgta caaataacag tcaagaggca
     7981 aaaagactat aaaggtttag aaagatacaa aatagtagat tcagctggtg ttagggaatt
     8041 tataaacaaa tctgattctt tatttattgt tgttccctat gagaagaatg gcgtaaaaaa
     8101 aataattttt cttggatcta gcaaggatga aaagcgaacg aaagagaaat tgattcacga
     8161 tggatcacaa tccaataaat ataagttaca aaagactagc gagtgattgc tagtcttttt
     8221 atttgtcctg aaggaggaat cacagtggca aaagctaaaa cgccatttga aaaagccgcg
     8281 tctaaaatct tagaacgccg cggtgtatct gaaacacagt ggaaacaaga agtccttgaa
     8341 aaagcacaat ttgacctatt acggggaaaa gacaaggact tagaaggata tatctatcaa
     8401 cgtgaacgtg aaaagcttgt tttcgatgaa attattaaac actaaaagga gcgaacaatt
     8461 ttatgtttga atcaattaca atgaattttt taacacaacc gaaagtagta gctgcggttg
     8521 atatttttag agcattacaa actgccgcaa ctagtttaaa aaacaatctg atgatttttg
     8581 ctgctgctgc ggccgttgct tccattgtta ttgggggtct tttcttccta tttggacgaa
     8641 gaggagctga aaatgggaag atgcacgtag gctatgtatt gatgggggta ggagttattt
     8701 tagcagcagc ttctattgtg gtttcgttat ttggattgtt tggacaaaca cctcaaggat
     8761 tataagaaag taaaaaagta ggagtaagaa gtagtcgata ctttttactc cttttttatg
     8821 aaaggagaat actcatgtac tttacgattg caggcaatac caaaacagat aaagagttaa
     8881 taaaaaaagc aacgaaggaa gtaggaaagc gtacttcttt tttaccgaaa acaattaaat
     8941 attctgtcat tgaaacggaa gaacagctac atgtgttagg gaaagtactg gaacacgaaa
     9001 gtcgatcttc tgttaagttg actctcacgt tacatgacac agaaggaaaa atagagaaag
     9061 ctggtacaga atacgttttt ggcccactgg attatcaaat ttcagaaggt ttctatccta
     9121 cattgatgga tttaatccgc cttgatgtgt ttaaagaaac ggatgaaact atgagtgtgg
     9181 aagagtctta tcgaaacgaa aaacttttac aagttttaga agaagagtta gagagcgacc
     9241 aaagaaaagt gaatcaaggg agtgaagtcg aagtaaatga agaagtacca tgggaacgac
     9301 caatatttcc aaagaaggag tcaaaagaaa aagagacagt ctatgttgac gaacaagctg
     9361 atattccgac tcagaaatcg atatttgagg aagagaacga tgtgatgggt caattggagg
     9421 tagatccctt tgaagaaaca atggaggatg aagagacggc cgaagtagaa gttgatccag
     9481 ttgaagcgaa tgaagagtca acatggactg aaggggattc tcaggaatat attgaaccac
     9541 ttatcagtga agaagtagaa aaaaacgaaa tagagataca aaaggcaact gtatttgatt
     9601 atattttgga tcaatatccg ccagagcatg aatggatgaa agaaaaaaac aaattatttt
     9661 taaatgagct ttattcgaaa tatcaattac cccaaaccta tcaagcattt ttagagagta
     9721 aagaagaatt aatctcacag ggtaaaggca cacttttaga acagttagag tctgtccaat
     9781 caacaaattg gcatgagctt gcacaagaag atttattagc cacctttgaa gaacgtcaac
     9841 aagaaacaga tcaagaaatt aatgagtatg cgacggaaca gtcgtctaat tgggagaaag
     9901 caaaagagga actaaataaa gaagaacagc gagtgattga agaagaagta caaaaaatta
     9961 aagagcgata cgaaaagaaa cgcgacaatg cgtattcctt gtgtgtagca aagatcaata
    10021 attttactca gacgaatcaa gaagcgttat cgaaagaaaa agaaaacttg ttaaaagaac
    10081 gtattaaaga acgaaaagaa gaattttacc gaggtttgcg tacagataag ctaaagatta
    10141 gcaaagaatt aaatgaacaa ctgaataagc tatatcaaac aacgactgaa gttcttatgg
    10201 aaaaatacga aaagatccag caagaattac aagtgcaaat tccaaaatgg aaaaaagagt
    10261 atgaaattca acaacaagaa ttgaaagaac aagaagaaaa acaacggcaa aaagaaaaag
    10321 agcgcgaaca attagaacta aaaaaacgtg aacaggatct tgcggaacaa caaatggcaa
    10381 tagaaaaaca acggttaaac caagaaaagg aaaaagaagc agctgctcgt aaggaacgtc
    10441 aagaacaaat ggatacattg cgacaagctc agttaaatta tctagcacaa cctcaaccaa
    10501 cgccttcagt agctgctgtt caaaccccaa tgaacaacca aacaaatcta actccttcac
    10561 cacagcgttc ctttaacgga tggatggtgg gatgtattgc aagtttaagc ttgcttctag
    10621 gtggaggaac agtatttgct tttaatcata tcaatcaatc ttcacaggct gattctgtgg
    10681 ccacaacgac gtcaatcaag gaagaagccc aagaacaagc aagaaaagag tatgaacaac
    10741 aattagaaga attattgaat cgtaccaatc agtcaacaac agagtcaagc atggaaaata
    10801 gcaaagagag tgattctagt aaggctaaag aaggatcaag cgattcaact gtacaatcaa
    10861 acacttccga aagttcagaa tcaaatgagg tgaaataatg cgtgttagag aaacaacgaa
    10921 gcgaaaagac aaacgattca atttagaaaa gaataggtat ctttctcatt tctatcgcta
    10981 tactccctca ggagaagagc taccagaaaa acatgacaaa cgcatttcga aaaagatttt
    11041 ttatagtgga cttgtatacc tccctttaga gtatttagta acaagagtac cagaagtttt
    11101 caaccgattg gaaattgtta agggaagctt acaaggaata aatctgtttc aattacagga
    11161 aatgccacag gtctttcatt ttgattcagc tggtttttgg aatccattta ctcctacaac
    11221 tagtttgggt acacgtttag caatttcgac agtttgtgtg gttccagctg tacttatggg
    11281 attgaaaatg gacatgtggt ttcgtcctct agcagatgga caaaaagggg ataatcgatt
    11341 aatgacctta aaagaaatcc aacagattta tccgcaaata cctgaaacaa aaactacttt
    11401 tccaggttat ggaggtattc caatcacaca ttacaaaaaa tattggtaca ttcaaacaga
    11461 tactgttaat acgtgtattg ttggtacctc tcgatcaggg aaaggtcaga ttgaagtgtt
    11521 agccaccatt gacaatttat ctcgtgcaga aaaacaaagt tcaatggtcg ttaatgatcc
    11581 aaaaacagaa ttatatgttg gtagtaaaga tgaactagaa gctagagggt atgatgtata
    11641 tgcctttaat atcaccgatc cactgcaatc aatgagtgat aatccattgg cactcatcgt
    11701 gaaatactgg aaacgaggag acatagatac tgcaacacag ttaaccaata ctttcacaaa
    11761 tacgatttat tatgatgcca acgcttcaga taacaaatac tttaacgaaa acgcgcagaa
    11821 agctgtcaat gcgcttattt ttgcgttgtt agaacaagct gatcaaacag gagattactc
    11881 caaattgaca ccgaataatc tcgttgagct tctttctgag attggcggtt ttaattatta
    11941 tccagatcca gacaatgaat tcaaacagtt gaatgcctta gatgaattta tgaatcagtt
    12001 gccacctgga aacattgcaa aaaaacaata tggggcaacg aaaattggat ctgataaagc
    12061 gaagggaaat attttatcaa cggcaatcac gggattgaat ccatttactc taacgaaaat
    12121 tgcaaagatg acttctcaat caacgattga ttataaatcg gttgggtttc caaaatactt
    12181 agatttgaaa ttacatgaaa gtcttttaaa tcaacgagtc gtagtagaat tttatcataa
    12241 gggaaagaaa tttcatgaag aacgtgtcaa agtaggctat aaaggatttt gtgaaatcaa
    12301 ttttgattgt catttagttg caggtgatca agtttgttta aaatacgatc atcaaggaaa
    12361 agaatatgtg gcttggtatg aattttctca aagagtattg aaagacgaag agggtaatat
    12421 cgtttatcgg aaaaaacgag gagaaaccca tttgccagaa ttagaaaaag aagccgttct
    12481 aacattagat gaggagaaaa gtaacttatt agtagagtct attcggatgc actataccga
    12541 taaaccaatg gctattttta tgcttacacc tgattatgat ccatctaacc atatcattgt
    12601 tagtattttt cttagtcagt tatataaaga attatctacg caatgtgtta agactaaagg
    12661 agataaatgt caccgcagga ttcattttat tttagatgaa tttgggaata tgccaccaat
    12721 ggataatatg gaaggaatca tgacggtcac agctggccga aacatgttgt gggatctgtt
    12781 tattcaatct tatcagcaac tttatgcgaa gtatggagaa caaaacgcag cgattatcaa
    12841 agaaaattgt cagacacata tttatattat gtcgacagat gataatacca tcgaagaatt
    12901 ttctaaaaaa gtggggaata agacggttga acaagagaac agtaccatca attcattagg
    12961 tatgaatact catatcaatc gcaatgttga ttcagatcgt atcttaactc ctgaaagaat
    13021 ttctactttt ttagaaggtg aaacgattgt actcgctcct ttaaaacgac aagatttgaa
    13081 aggtcataaa atacggccgt atcctatttt taatcatggg gaaacgaatc ttccttatgc
    13141 ttatcaatat attgaagatt tcaatccatc gtgggatatc aatgatatag acatacattg
    13201 tacacatgcg gacttggatc ttaaggcttt acaaattgat tatgattatt tcctaccaga
    13261 tgaatatgct agaaatctct atcatggact gcatgatcaa ttagaagaaa aaaacgaaga
    13321 aaatatgttt tataaaatcg ttcaatctct tgagaatgag acggctaaaa aattgttaat
    13381 tcaattttac gaagaacaag cagcagatcg acttcaagaa tatgttgaaa agttgatcaa
    13441 aaataaagaa atttctgaaa tgattgggaa acggttgtta ttggaatctg aaaaggtggt
    13501 gaaagaatga tcattggagg aattttttct ggcatagccg actggtggtc aggcttattc
    13561 ggagaagggg atgcaaaccc caaaaccgta acgaaagtct tacaagatta tggtaattat
    13621 ctgcagtatc aagatgtatt tagttggatt tggaacactt ttctgtgggg attgattaaa
    13681 ggattatata atctcaatac tttactagaa aaaaccattt atcaaagctt tgatttaaaa
    13741 gacttattga acgccgctgg ggtaaatgca ctatttcaaa gtttgattag taaggtattg
    13801 gctctttttt gtgtcgtcac attaatttat ttaggcttga aattttttat gagtaaacac
    13861 ccaccaaaag tcaaaaatat cttggttaat ttatttatgg ccatgatctt gatttttggt
    13921 gggagttctt taatcgatga agggttaaat atcagtaaaa gcttttacgg agacgtcact
    13981 gcagcaaata aagcagataa aacgggttca cctgcttttc aaattattca aaataatgtg
    14041 atcgatgtca gtacattact agcaactgac ccgaataaag tagaatccct gccaacagac
    14101 aaaagaaatg ctttaacagc gaaaaatttt aaaacagcga atatcaatgg gattattact
    14161 cctaaacaag ctaaagaaat ggcggataaa gataagagtg gtttatcaga tttacaaaag
    14221 gatcggttaa gagcgttaca ataccgattg gaactagatg ataatgggaa agaaattcct
    14281 gttcaaatta gtgacgaagg cttagcaaaa tatgtctata cttcagggta tcgtcgatac
    14341 agtagtaaac caggtattat cttagctggt gaatcttctt tggcggtggc ctatttattt
    14401 atcctattta cgatcgttac ttgcattatt gagctggtat ttaaaaaatt ctatttagtg
    14461 attgccgcgt caacggactt tgagacaggc caacggatga aaagtgcgat agaagatatt
    14521 tctcaatcct tcttgttatt ggcgtttacg attttagaat tacgaatcta tacattaatg
    14581 ctttcaggta tcggtgattt gcatgcggca ggaaagatca atggattctt gtatgttgtt
    14641 gctttaattg tgctaactat tgcgctattc aaaggctctc aatcggttac gaagattttc
    14701 ggtgttgata cgagtctgaa aaacggcagt aattctcttc ttagcatgtt tgccttagga
    14761 aatattgcga aaaatgtggg tagcggagct aaaaaccttg caagtgctgg taagagtggt
    14821 ttaagccatt taaatgatat tcgtaaaaat ggatttggcc atcgaaaaga tgttacccct
    14881 gataacgggg aagaacaatc agacgataaa gctacaactg tacctaatag taagaaaaca
    14941 gtttccgaaa tgttcgggaa agcgaaaaat ggtgtttcta aagccgcaga aggtcttggt
    15001 tacgtcaatg aacgtgggtt aaaaggaaca gctgaagatc ttacagaaaa agccaaagac
    15061 accttagaag agaaattaga cggcacgaga gctaaagaat tggctgaagc tgtgaagaat
    15121 ccatccgaag cactagaaaa acaaaaagaa aaagcttcag aagtcaaaga aggactaaaa
    15181 gatgcgtatg gtgaaggaca agttaatgca gcagtaaaag ctaatacaac aaccgaaaaa
    15241 gaaaatcaag aaggtatagt gattccttca gtcaaagatc accaagaagc gttgcactct
    15301 aatgaaaatt cgattgaaga gaaaaattcc atagaagaaa gaaattcaga agaaggaaaa
    15361 aatggacagt taccaaaaac tgatccagaa caatatcgct ataaacctaa cagtgacctt
    15421 gaaaatgaga agcgaatttc agatttatca aaagtaaatc aagaagacaa agcagaaaat
    15481 gagggacagt taccaaaaac tgatccagaa caatatcggt atagatctgt taactccgaa
    15541 aatgaaaaac gcatttcaga tttaccaaaa cgaaatcaag aagtacaaac ggatgaggat
    15601 tccatcgttc tgccaaatga taatggaatc gaagttggaa ctgaaaaatc aacgaaaatt
    15661 ggtttaccta aggcacaaaa tttaccaatg aaagatagtc gtttgaatca attcaaggag
    15721 aatgatcaaa gctttgttca ccaatcaact gcaggtactg ttcaaatgaa accaagtcgt
    15781 ttgcctgaaa atggaaatct gacaactgtt tcttacaatt tatctgaaag tggtgagaca
    15841 gggaatgcgt ccgttcaaaa tgttgtgaca agagaagctg caccaaaacc tattcaaaac
    15901 gttcatacag gttacaagcc agcaaatgtg gcatttactg caccaacaaa aataaatgtg
    15961 acaagagaaa ctaaaaacag gaacaatgca ttgaaagaac aactattgag ggatatcaaa
    16021 caggatatca aaccataaaa ggcctacaag ttaggccttt tatttatatg aaaaggagaa
    16081 atcaattatg gataaatata taaacatgaa aaaccctcca agatttagtg gagaatggaa
    16141 gtttttcgct tttttctttt tagatctatt aacgcttggt ggctgctttt tttgggcgaa
    16201 aatggtcaat aatcttgtcc acttaccact cttttttgca ctcttgaatt atggcttatg
    16261 gattgcgata gcgttagtga ttgtttggcg accctataat aacccaggtc aacgacaaat
    16321 ttttgcgatt atcgagtcta ttttttttgc ggacaagaac cattatcatg cgttagatcc
    16381 taaccgaaat tatcatggta cgtataagga ggatgaataa atgcgagtca aaggacaaaa
    16441 ttcagttgaa aggaaatcca ttttgcagtt tggaaaacca gaagagcgct ctatcttgga
    16501 cttatttccg tataaatcct gtacagaaga gggagtctta attactagag aagatcattt
    16561 tcaacgttat tatcgtgtag tttctacaga cgtggaagga ttaaatgaac aagagaaact
    16621 agaacgaatg aatcagttga ccaccgtcat gcgtacgtat gtgccaacaa ttaagttagt
    16681 tagtttgacc acagagacgg atctaacgga acaaatcgtg caaaagcggc aattattaga
    16741 taaaaatcgt atggaacagt ccttaggaag aaatctacaa cgattaaaaa aatatgaaaa
    16801 gaaattagtc gaagaaatcc aggaattgaa acgagcagaa caagaacgtc ctgacttaag
    16861 cttctttttc gtcattgaag gaaagacaat caaggattta caaaccagaa accgtcagtt
    16921 aatgcgatct agtggcattt tagggttaaa accactctac aaaaaagaat tgatcaagat
    16981 tctgtatcga atgaacaata tgaatgatga gtaggaatgg agaagagaga atgaaatatc
    17041 cgttgatatg gttagaaaaa gatgttccga aggaagagac cactagaaaa tttcctcaca
    17101 ctttaagcct gggtaagttg ggaaaagggc aatcttttaa ccaagtaaag aatcaaacaa
    17161 gaagaggtga ctgtacatgt tagaagcaat taaaacagct ttttcaccac aaaatgatca
    17221 aaaagtactt gatccattaa agcaactagg aaatcgggaa atagaaattt taaaatccaa
    17281 agggtatgat ttagattttt taaaacaagt gatgccacaa ggtgggttaa attttgatga
    17341 agaatatgtg acgtatggta atggttattc acgatgtgtg acagtttatc actacacaaa
    17401 aaatcctacg cttttttggt tggccaatct catgaacaat cctcatacag tggccacact
    17461 cgatgttagc acagatgaaa agcaaaaagt gattgatcaa atcaatcgtg ccttagatga
    17521 attaaatgat cgatcagata atgaacgaaa agcgactgac cgaaataaaa gcgcagaaga
    17581 atacctggaa ctagcggaat atgctcgtaa attgacttct aatggggaaa tttctaagca
    17641 agtcaaagtg agagtacatg tttacggaaa cacgattcaa gaagtagatg aacgagcgca
    17701 ggaactgatc gaaaatctaa aagggatgga ttatcgtgcg gtgacttatc tgcataaaac
    17761 caaacgagat tggcaatcga tgtttaccat gttcgatgaa caagctgaat ggttcggaaa
    17821 tatcaaaggg caatcactac catcgttaaa cattggcgga ggtttcccgt tccaccacca
    17881 aacgctaaaa gatccaggag ggacacaata tggggaaacc atgacaggcg gcccatttgt
    17941 ctggaatgcg acgtataaaa atagtgtacg gttgtctact aataccttgc ttctaggaat
    18001 gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg gaagcccatt tagctgcagg
    18061 agactttatt tggggctttg aaaagggaaa agactttatt cctttcttaa aggaatataa
    18121 cggtattatg gtgcgtttag acggatctga cggaatgatc aatcctttag agatatttgc
    18181 cacacggacg tatgatgaag catccagttt atatgacgat ggatcagtca aagatttgaa
    18241 gatcaatgaa gcagcgtcct atcaaacaca cttagacaaa gtggtctatc aggtacagtt
    18301 agtgagtcct cagctaaaag gaacgatgaa agcagaattt aagacctact taaatcgttt
    18361 ttatgaagag tatggaacag taccacgcgg ctttacttcc tcaaattcgc gttcaaatac
    18421 ggagacgcaa gtcacaggta aagatccaga agcttatccg acgttcaaag aatttttaga
    18481 ctttttagga caactggaat taccaggtgc ttcccaagag aaaaagaatc gaaaagaaga
    18541 aatggaatcc atcgtagaat ccttatgtga gacgtatggc atgatttttg atgggcatag
    18601 tacgattcga caccttgacc aacaacagtt ggtctttttt gacattgact ctattacagg
    18661 cttagatcga ggggtacaac aatgtcaaat gtacatggct atgacgttga tttggaatca
    18721 ggcattgatt caaggaagaa gacaacgacg tttaattaaa gaaaaacaaa tggaagaaca
    18781 agacttcaaa cgcttcttgg cgatgattga tgagtgccat aatgcgatta atccagattt
    18841 tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat
    18901 gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt cttctgataa
    18961 cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta tgcgtatgga
    19021 tgaatctatt ttacgtcata tagaaggatt agtgggtaaa tcaatgacga actcagaatt
    19081 agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct caaaagaaac
    19141 cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata
    19201 atggaaaacc aaaatgaaag tttaatcaaa cagtatgtta agcgtcgtgc aaagcggcgc
    19261 ttatttttgt gggtgtttgg tacttctgca ggtttgatta ctatattgat cactgtgttt
    19321 gtgacccttt ttctaatact tgcggcaggt tctattgata attctgattc aagtagtggt
    19381 ggcgaagctt ttacaggaga atattcggaa ggcttaccca tttataaaga aatcaaagga
    19441 agaggccctt tttctgatga gattgcgcaa tatgctgtag gtgcagcagt gaagtataaa
    19501 ttattaccaa gtgtgatcct ttcgcaatac ggttatgaat cagcgtttgg tacgtcggcg
    19561 tccgcaagaa atgatctcaa ttattttggg attacgtggt ttgatgggtg tctgtttcct
    19621 aaaggaactg cgcgtggaat tggtggcatt gaaggtggct ggtacatgaa gtttcccaac
    19681 tcaaaagctg ccttctccta ttacggcttt atggtagcca cacaatcaaa ttttaatgct
    19741 tgtgtcggaa ataaaagccc tggcgcaagc ttattgatct taggacgagg agggtatgcc
    19801 gccgcaggaa ttactgaaga cagtgcctat tataaaaatt gtatgtctat tattaataca
    19861 aataaattga tggagtacga tgagtttgcg attaaacact ggagagaagg cggcggaact
    19921 ggtggtacga ttacaggaag ctggggcaat ccattcccag gaagtagctt agataaaaat
    19981 tcgttcagtg gaggacaact ttttgggaaa aatccaggag gagaattccg acctaacggg
    20041 ttccatgatg gactagactt tggttcagtc gatcatccag gaagtgagat tcatgcggtt
    20101 catggcggaa aagtcgtgta cgttggcaat cctggtattt caggtttggg tgcgtgtgtg
    20161 atcgtgatta attacgatgg cttgaatatg gtgtatcaag aatttgcaaa tagtacagga
    20221 aattctcgag tgaaagtagg cgatcaagtg aaagttggtc aagtaattgg gatacgagat
    20281 acagcacact tgcatttagg atttactcga atggattggc gtcaagcaca aggacatgca
    20341 ttcattgatg atggtacatg gatcgatccg ttaccatttt tgaatagcag caaaaaatag
    20401 atcaggagaa aaaatatgga aaataaaacc ttaaaaatcg tcttaacagc tgcaattagc
    20461 gttagtttaa tcggcaatat tgcgttgttt tggcagctta aagaagaaca agagacaaat
    20521 acctataaaa ttgaagaagt ggctaaaaac gcgtctgata agctcaaaaa aaattcttct
    20581 gaggcacagg cgaagatcga caagttaaat caaaccatca atgagaaaaa ccaagaaatc
    20641 gatcagctga agcaacaaga tggcacaaaa gcaaaagagg aagtaactga agcacaaaga
    20701 aagacggcag aagagttcgg tcaattggcc attgataaat cattaagcag aaatgaatta
    20761 acggaaaaac taaaagatgt cgcaacccaa gaagtgatcg acaaattaag tcctgcagac
    20821 gatgatcacc aacatgacga ctacggaagt tactctttta cattaggtga cgtgcaaact
    20881 tacgctcaaa caagcagtgt aaaagaggaa caaaattttg tcgtatttgt tgattataca
    20941 atcggcaatc ctcaatttaa agatgtcaaa ccacaaaaaa taaaaggtgg attaactgtc
    21001 actgaaaaac aagtagatgg tcaatggaaa gtcacagact ttagttattt cactcgttag
    21061 gaggaagaaa atgagtaaga aaaaaggagc aagacaacgt tttaaaaaac gaaaaagtta
    21121 tcttagatgg ctggggatct ttatgattct cggctttttg attcttgcta gtccgattta
    21181 cacacataaa aaggtagaca taccagatga agtaagcacg aacgcatttt taaacacgaa
    21241 ggaactgacg atggtttcaa aagaaaaagg agcgaaccag caattagttt ttgaatttgc
    21301 gatcgacgat gaagatcaga gcttattaaa agaactagct aatcttaatt atcgtgtaga
    21361 agtaaagaca gctaaagggg attatcaagc aataaaagcc aaagtgatca aggtgtctga
    21421 tgattatttt gtagtgaagt tagcacatgt accagaagaa tatatcgcca tgcgtttaac
    21481 cattattcct gaaaaaattg atccaaaagt ggatatgcaa gaaccacaag atctgattta
    21541 ttacgttcac gaagataagg ttaaagatca agtgaaagat gaagattatg aacaacatgc
    21601 gatcaattac aaagtgaaag gatacaaaaa agaacaaaaa gcagcacaaa aacaaatgga
    21661 atccttccaa gcaaccattg atttgaatga agaattagtg aagaagctaa aagatcaatt
    21721 gccttatcaa gtcgcagatg atcaggaaaa tactgaaaac aagattaata gctaccagca
    21781 agaaattgaa acatcgaaaa cgcaaatgaa agatcaagaa gagatcattc aaaaacttca
    21841 agaaaaaatt gatcgtatga caaaagaaaa gatttagaag gacaggtgga gtgaaaaaat
    21901 gaagcgaaca aacttggctg acaaagatac tttcgctgag cagaaagtat tgattaaagc
    21961 ctatctcaaa gttgtaaaag ctaaagaaat atgtcgagaa gtgaatttat ctaatacaac
    22021 aatatctgag ttgaagaatg gtcgaaagaa tttagatact ttaaaattca gatcatttga
    22081 agctctatat gtagctgcta aaaaatattt tacagctgat aacaaataaa aaacaggagt
    22141 attttatgtg aaaaaagtat ataaaaaatg cttatgaatc agctatatca ccttaaccat
    22201 ttgttaatga gatgggagaa aacattaacc acgattctaa cgcaaacatc aagcacataa
    22261 aacatttaac actcctcctt tctggctgaa gatttcaacc aaacaggaag acagcgtatc
    22321 aattatagca tacacttttt cttaaattca tttttcatat gacaaaatat ttccttctaa
    22381 tcgacaaagt tagaaggatt ttttattgtc taaatacaca aataacctta atttataaag
    22441 agcataagga aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta
    22501 cgtttctcaa aaataattgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagc
    22561 aaaggatagt aaaaggatag aagcaggata gcgaaaagga taggctcctt ttcgctatcc
    22621 tttttaaatt ggaggaatta atatggaagt aagaataaga aatgtagatg agaaaatcgt
    22681 aagagaaatt gatcgaagat gtgacgaact aagtaaccga acgggtaata aatggagtcg
    22741 taacgattat ttaaagctac tcgttgaaaa tgattttgat cgtcctttga tggaatataa
    22801 acaagataaa tttgatcaat tattaaatca gttaatagat aaattttctt ttgttcaaag
    22861 tcataataca aaagtattag aagaatatat ttgtcagaat aatcaaatta tcgaactttt
    22921 aattgaacaa agtagaggag gagaagttta acatggccaa agttatagac aagcatattt
    22981 atttttatga aacacatata gatgttctag atgaaattat tgcaactcat gcagaggtta
    23041 aaaactatag tgaagccgtt cggtttctat gtatgaatta tgattcagaa gtggagaaaa
    23101 aagtaaattc caatcaagca aagttgaatg caatgggaaa agaaatttct attttaaccg
    23161 aaatggtttc agagtttgcg gatctccatt tgaaagatac agggatttta attggtacaa
    23221 attcaactgt ttaccaagaa gcaaaagagc gtgtggaagg caagataaaa gcaaatcaaa
    23281 cgaagaaata ttctcctaag ataaagaaaa caataagcga atcagtgact acaaaaagtg
    23341 aagtatttga tccaacaagg ttcttaaaac gataaaaatt agaaggagaa tgtgatgagt
    23401 aaatcacctg cagtcattct cacttgtcaa tttaccttgc ccaataaaaa atctttttca
    23461 acatacattg attatatgac aagagaaaaa gcgctggaag aaaaggaacg acgtacgccc
    23521 gaggaagaac aggaactgcg tctaatcaag aatgctttag aggattttta tattcctgaa
    23581 ggagaaactt tttcggagag taagacgggt aaagaattca gcgaaacgtc gaatgaagcg
    23641 caatcgttga tgaaacatgg aatcaatttt gacaagttag aagaacaaga ttttaccaaa
    23701 tatctttctt atatgacacg tcactatgcg ttagaacaaa agaaagtact gacgcatagt
    23761 gaacaaaaag aataccaacg cttgaaccaa gcaataaaaa aatatcaaac agagccacac
    23821 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
    23881 agtgatctga aggagatcaa gcaaaccttc caaagaggac aacaaaaagg ctcaatcatt
    23941 tatcaagatg ttgtctctca tgatcattcg tatcttgaaa aaataggatt gtacgatccc
    24001 aaaacagata ctttagatga agaaggttta aaagcagctg gaagaaaaat gatggagaca
    24061 ttgttcgaga aagagcaact gaatgaaaca ggctactgga tggcaacgat tcaccgaaat
    24121 accaaacata tccatattca ttttgcagtt gttgaaaaaa aaaatacgcg taaaccatac
    24181 actgagattg aaaatggtgt gagttatatc gtaccgaaag ggaagagaag gcaagcaaca
    24241 ttagacgaaa tgaagactaa ttatgtacat gagttagaac gtttttctta tgaaaaaaat
    24301 cgaatgggta tggagaaacg aaatttattg acacggaaaa gtgatttgcg taacacctta
    24361 accaaagtag tgaaagagcc agtacgttat gatcgacacg caatgagctt attaactgaa
    24421 gtatataaaa acttacctaa aaagcgttcg gaatggcatt acggggatga aaatcgaacc
    24481 aaactttcac catacacaag agagaaactc gatgagttaa cccaatatgt gttaacaaaa
    24541 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agcaaaaagt
    24601 ctttatgggg aaagcaagag agaatcaaag gatgcagaaa aaaatgctct ctttgattta
    24661 aaaaagcgca caggaaatgc cattctaaca gaactgaaga aacaggatag tcaaataaaa
    24721 cctttaatag aaaggatgga tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
    24781 cttaaacagt tgccaacgaa tgaatacaaa tatagttacg aagaattcat gaagcgcagg
    24841 aaacaattcc aacaaagagt tgtcacgcaa cggagtttgc gtcaattaaa tcgaacgatt
    24901 aatgcccatg aagaaaaata ccacgcaagt aaagcatacg aagagcaaca acgaagaatt
    24961 caaatggaac aagaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga
    25021 aagttataga aggatcatta agagaagaac gaatcgaaca acttcccgat tgtttagaaa
    25081 aagagcattt gtttcagata agagaaatgt tgataaacga acagtttgat ccaaatcaat
    25141 ttttagtcgt tgagtaccca gctactggtg tgtactgttg taatcatgtg aagggtgaaa
    25201 aatattttat cattcaagaa tatgagggca agttagcgcc ttattatact acttgggaaa
    25261 tgaatgaaga ggggatcaat aacttcccgt gtaaaagcat tgaagagtct atttcactca
    25321 cagaatgtta gttgttaagt gaataaaaga agtaaaagag tcctgaacgt gattgtccag
    25381 gactcttttt tttcactgaa ttgttttccg ccaattcatt gagtggtgcg atttccgtct
    25441 acattatatc aaaagaaagg aggaagaaga aatggcttat tcaacgattg gagttgttgt
    25501 tgaaaaaagc agagacaatc ttgtttttgt gacagaaatt caaacaggca gagcttttgt
    25561 agtcacagac aaagcggcaa aagcatatca aagtggcgat attttaacgt taaatctgac
    25621 tacgaagacg ttcgtcgatg cggcagaaga ttatccatt
//
LOCUS       DAZUMI010000030        25575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000030 DAZUMI010000000
VERSION     DAZUMI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.030"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..902)
                     /gene="deoD"
                     /locus_tag="ACG81C_001066"
     CDS             complement(195..902)
                     /gene="deoD"
                     /locus_tag="ACG81C_001066"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5064243.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(919..1737)
                     /locus_tag="ACG81C_001067"
     CDS             complement(919..1737)
                     /locus_tag="ACG81C_001067"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5064244.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1761..2927)
                     /gene="deoB"
                     /locus_tag="ACG81C_001068"
     CDS             complement(1761..2927)
                     /gene="deoB"
                     /locus_tag="ACG81C_001068"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5064245.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3026..3982)
                     /locus_tag="ACG81C_001069"
     CDS             complement(3026..3982)
                     /locus_tag="ACG81C_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064246.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3982..5115)
                     /locus_tag="ACG81C_001070"
     CDS             complement(3982..5115)
                     /locus_tag="ACG81C_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064247.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5108..6670)
                     /locus_tag="ACG81C_001071"
     CDS             complement(5108..6670)
                     /locus_tag="ACG81C_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064248.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6882..7964)
                     /locus_tag="ACG81C_001072"
     CDS             complement(6882..7964)
                     /locus_tag="ACG81C_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5064249.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8030..8428)
                     /locus_tag="ACG81C_001073"
     CDS             complement(8030..8428)
                     /locus_tag="ACG81C_001073"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5064250.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8449..9111)
                     /gene="deoC"
                     /locus_tag="ACG81C_001074"
     CDS             complement(8449..9111)
                     /gene="deoC"
                     /locus_tag="ACG81C_001074"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5064251.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9157..10458)
                     /locus_tag="ACG81C_001075"
     CDS             complement(9157..10458)
                     /locus_tag="ACG81C_001075"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5064252.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10599..11615)
                     /locus_tag="ACG81C_001076"
     CDS             complement(10599..11615)
                     /locus_tag="ACG81C_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5064253.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11923..12528)
                     /locus_tag="ACG81C_001077"
     CDS             complement(11923..12528)
                     /locus_tag="ACG81C_001077"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5064254.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12724..13647
                     /gene="coaA"
                     /locus_tag="ACG81C_001078"
     CDS             12724..13647
                     /gene="coaA"
                     /locus_tag="ACG81C_001078"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5064255.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13706..15271
                     /gene="guaA"
                     /locus_tag="ACG81C_001079"
     CDS             13706..15271
                     /gene="guaA"
                     /locus_tag="ACG81C_001079"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5064256.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15762..17393)
                     /locus_tag="ACG81C_001080"
     CDS             complement(15762..17393)
                     /locus_tag="ACG81C_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5064257.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17407..19080)
                     /locus_tag="ACG81C_001081"
     CDS             complement(17407..19080)
                     /locus_tag="ACG81C_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5064258.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19093..19410)
                     /locus_tag="ACG81C_001082"
     CDS             complement(19093..19410)
                     /locus_tag="ACG81C_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5064259.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19435..20835)
                     /gene="lacG"
                     /locus_tag="ACG81C_001083"
     CDS             complement(19435..20835)
                     /gene="lacG"
                     /locus_tag="ACG81C_001083"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5064260.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21072..21857
                     /locus_tag="ACG81C_001084"
     CDS             21072..21857
                     /locus_tag="ACG81C_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5064261.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21860..22552
                     /locus_tag="ACG81C_001085"
     CDS             21860..22552
                     /locus_tag="ACG81C_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5064262.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22826..23197)
                     /locus_tag="ACG81C_001086"
     CDS             complement(22826..23197)
                     /locus_tag="ACG81C_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5064263.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23597..23827)
                     /locus_tag="ACG81C_001087"
     CDS             complement(23597..23827)
                     /locus_tag="ACG81C_001087"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS5064264.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23921..24259)
                     /locus_tag="ACG81C_001088"
     CDS             complement(23921..24259)
                     /locus_tag="ACG81C_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064265.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25187..25303)
                     /locus_tag="ACG81C_001089"
     CDS             complement(25187..25303)
                     /locus_tag="ACG81C_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5064266.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7455 a         5611 c         4511 g         7998 t
ORIGIN      
        1 tcaaacatga aaagctgttt tttgaaattg ttccttgtga ctcagagtta aacggcagat
       61 tcacaacctc ttggtaaacg gtgttcaaaa gcttacattt gcaataataa gtccagccaa
      121 gtcaaaaaca tgaggagcta tttttgcttg gatgttctta ttacttaatg cagggcagct
      181 ttttcatttc cttcttattc tgctatcgct gtttccaatc ctacttcgat catttcattg
      241 aacgtagatt gacgttctgc agctgttgtt tcttcaccgg tgatcaaatg gtcgctaacg
      301 gtcatcaaac ttaatgtctg cacatggtat ttcgcgccta agtaataaag agccgctgct
      361 tccatttcca ctcctaaaac gccaagttca gctaattgga acgtctctgt caaatcatct
      421 ttgtagaaag aatcttccga aagaacattt cctacatgaa ccgtatagcc cttttcttta
      481 gctatctcat atgctttcag taatagattg aaatcactga ttggaggaaa atgaaaggct
      541 tggaaatttt tttcgatcat cgaagaactg gtcgctgcac cttgtgctag tactaaatca
      601 cgaacatgga cgtcttttga caatgcaccg catgtaccta ctcgaatcaa tttctttaca
      661 ccatacgact ggatcaattc atgggcatag atggtggctg atggcattcc cattcctgtt
      721 ccttgaacag aaatacgttc tcctttatat ttgccggtaa agcctaacat accgcgtacc
      781 tgattgtaac aaactggatc ttccaaaaaa gtctccgcaa tatatttagc tcgcaaagga
      841 tctcccggaa gtaaaatctt atcggcgatc tcgccttctt tcgcttcaat atgcacactc
      901 attttattgt tcctcctatt acaataatgt caatgtttct ttaaccaatg ctttaaaaga
      961 ttgtttgact ctctgagttg tttctacaac ttcttcatgg ctcagattcg cttgcatacc
     1021 tgctgctaag ttggtaatac aagagattcc taacactttt aatccgctgt gagctgctac
     1081 gatgacttct ggaacagtcg acatccctac tgcatcggct cctattgtcc gagacattcg
     1141 gatctccgca ggcgtttcat aagttggtcc agaatagccc atatatacgc cttctttaag
     1201 atccaaacct agtttttctg ctgctgcttt tgctgttgct tgatagtcag gcgtataagc
     1261 ttgactcata tctggaaatc tagggcccat tgcatcttca ttcggaccga tcaaaggatt
     1321 ttcaccagta aaattgatat gatcggtaat cagcatcaaa tctcctggta caaatgattc
     1381 gttgacacca ccacatgcat tagtcacaat gactgaatgg gcccctagtc cggccatcac
     1441 acgtactgga taggtcactg tttgcatgga atgaccttca tagtagtgga atctcccttg
     1501 catggctaac actttcttgc ctgccagaga cccataaact agctttcctg catgcccaac
     1561 tactgtcgaa acaggaaaga acgggatctc atcataagaa atcattacag catcttcgat
     1621 ttcatctgct aattcaccta atcctgaacc tagaatcaaa ccaaaatcaa tttcttttac
     1681 tcctttatca aggataaact gggtcgtttc ttgtaacatc tcgcttaact tggtcatttg
     1741 ctttctccta tttaaaaaga ttattttaat aatttcaaga agctttcgcc attttcagtc
     1801 gaagaaacac taaagttctc agcaatcgtt gctgaaatat ctgaataata tccttgaggc
     1861 aatgcccctt gttctttcat ttttttactg taaaccaata acggtacata ttctcgcgta
     1921 tgatcggtac cagtaaacgt cggatcgttt ccgtgatcgg ctgtaatcat tagcaagtcg
     1981 tcttcttcca tcgcttcaag caattctgga attcgtaagt caaattcttc aatcgcatgc
     2041 gcatagccga ctacatcccg acgatgaccg tataatgcat caaaatctac taaattggta
     2101 aagctgattc ctgaaaaatc gtttttcata actttcagca gctggtcaac gccatccata
     2161 ttgcttttcg tgcggacaga ttcagtgatt ccttgtccgt tgaaaatatc gttgatcttt
     2221 ccaacagcaa tcacgtcctt tcctgcttct ttcaaagaat caaggactgt gtgtccgaat
     2281 ggattcaacg cataatcatg gcgattgctt gttcgtgtga agtctcctgg ttcacccacg
     2341 tacggacgtg caatgattcg gccaatcata tacggatcat cttttgttat atcacgaacg
     2401 aattggcaaa tccgatataa ttcttctaaa ggaatgattt cttcatgtgc cgcgatctga
     2461 aggacaggat cggcagaagt atatacaatt aaatcgcctg ttttcatttg gtgttcaccg
     2521 taatcgtcaa taacagctgt tccgctataa ggtttgttac atacgacttt acggccagaa
     2581 aattcttcga tttgtttgat cagatcatca gggaatccat taggaaacac acgaaatgga
     2641 gttttgatat tcaatcccat gatttcccag tggcctgtca tcgtatcttt ccctacagaa
     2701 acttcttcta atttcgtcgc atagccttga tgatctttca ctgattccac accttcaaga
     2761 ggacggatcg ttcctaaacc aagctgctct aaatgtggaa cgttcaatcc tgcttctttc
     2821 gcaatgtgtc ctaatgtatc agaaccaaca tcaccgaact tatcggcatc tggtgcttct
     2881 ccaattccta ctgaatccat gacgatcaga tgtacgcgtt taaacatgat tattccttct
     2941 ttctttttat ttttattgcc tttaaaacaa aaagggcgag acgaaaaaca cttcccgtaa
     3001 cccacccttt ttattcgcta aaagattatt ttgatttgat atagttcttc ccgtttgctt
     3061 ttggaccaga agctttccct aagaaaacaa ccagcacaat gattgtcagc aaatatggag
     3121 caatttgcag caatactggc ggaatcgaag aaataactgg taattgtgcg ccaatgatac
     3181 tcaagctttg agcaaaacca aagaacaatg aggcacccat tgcccctaaa ggattccact
     3241 taccaaagat catagcagcc atggagatga acccttgtcc aacgatcgta ccagctgaaa
     3301 agttaccgga aatcgactga gcaaaaacag caccgcccat accgcctaac aagccagaaa
     3361 tcagtacgcc tgaataacgc atagcatata cattgatacc taatgtgtcg gcagcttgag
     3421 gattttcacc aactgaacgg aggcgcaagc cgaatttcgt cttgaagatg atcacccaag
     3481 ctaggacagc aatcaaaata gcacaataag caggtaagaa tgtattttta aagaaaattg
     3541 gtccaatgac cggaatatct gctaatcctg ggaaagaaaa ataccccaga ttctgcgtga
     3601 tgttatcagt ttgaccttta ccataccatg ctttgatcaa gaaaacacct aaagcaggtg
     3661 ccatcagatt aattactgtc ccgctgataa tatggtcagc acgcaaactg actgttgcta
     3721 ctgcgtgaat cagagaaaat aggataccta cgacgccacc cactaaaata gcgatccatg
     3781 gtgtcataga accaagtgat ggactcatcg tcaagttgaa aacgatggaa ctaaaagcac
     3841 ccatgaccat gattccctct agccctacgt ttacgatccc gcttcgttca gaaaaagttc
     3901 cgcctagtga cgttagaatt agcggtgttg aataaaccag tgtagaagta aggatcaagg
     3961 caattaattc gatagtcgac attatagttc gcccccttca tggttttgat tcgttggttt
     4021 cgattctatt gtcgcaacga tctcttcttg ttttttccct tggaagactt tagccatgac
     4081 gaaacgaatc aggaagttga tcccgacaaa gaagataatc aaagcaatcg atacattgac
     4141 gatctcaaac ggaacacctg ctcctgtctg catgccaagt ccaccgatct tcaaaatact
     4201 gaacaagatc gctgataaaa gaataccgat tgctgatcca gaaccaagca aggaaacagc
     4261 cattccgtca aatccaatag atagcgaagt cgtttgaaca aagaagtttt gatacgttcc
     4321 taatccttca accacaccgc caagccctgc caaagcgcca gaaatgacca tcgacaaaac
     4381 gatcgtacgt ttactactca ttcctgcata ctcagaagca aaaggattca atccaacaga
     4441 acggatttca aatcctaaag tcgttttctt catcagcagc caaacgaaaa taagcgcgat
     4501 gattgctaaa aataatcctg agtttacgcg agagccgcca aaagcttgtg ttaaccatgg
     4561 taaccgcagt gaggcatttt gactgatcat attggaagag tccaaacttg agcgatagct
     4621 ttcaggcatc acttcatgta aagcaaatgt gcttacgtat aataagatat agttcatcat
     4681 gattgttacg atcacttcac ttgttccaaa aaatgctcga agtaaacctg gaacagccgc
     4741 tgccatcgca ccagctaagg cacctaagat cacgcataac ggaagcaaaa caacttttgg
     4801 cagatctgga aacgcaagtg ccacccagac actgacgatc cacccgcaaa gtgcttgtcc
     4861 agaaagccca atattgaaaa acccagctga gttagctaca gaaaaaccta atgctgtaaa
     4921 aatcaacggc gttgcttgcc ttaacgtttc acctatccct ctttgcgaac caagggctgc
     4981 atctagcata gaagaataac cttggatggg ctgaaaacca aaagcagcca tcaatatcgc
     5041 gcctaagatc aatcctaaaa caactgaaag tacaggcact aataaattac ggaatttttc
     5101 gctgcgatca ttcatgtgcg ccagccccct tttctaactc ttttcgtgct tcttctaagg
     5161 tatagcccgc catcaataga cctaattcat tttcagatgt ttctttcgga tcaacgatac
     5221 cgacaatttc tccttcatgg ataacagcaa tacgatccga aacatttaaa atttcttcta
     5281 attcgaaact gatcagtaac actgctttgt atttgtccct ttgttcgatc agacgtttat
     5341 gaataaattc gatcgcacct acatccagtc cacgagttgg atttgcaaca atcaacagat
     5401 caggatctcg gtcgacttca cgagcgataa ttgctttttg ctgatttccg cctgataaag
     5461 cttttgcggg aacaagttca ttcgttgttc gaacatcata ttcttcgatc aattctctgg
     5521 catgttcatt cattactgaa taattcagca tgccgttttt actgtaaggt ttttgatgat
     5581 aggtttgtag tgcgatattt tcagataatg tcatatctag gactaacccg tatttatgac
     5641 ggtcttctgg cacgtgtcct acaccatgtt cagtgatttt tcggggtttt ttattagtga
     5701 tgtcttcccc ttttagcttg atatgtccgc tttctgcctt tcgcaaacca gtcaaagctt
     5761 ggatcaactc cgactgcccg tttccatcga ttccagcgat accaagtact tcgccagcac
     5821 gaacctctaa gttcaagttt ttcacggctt ctaatccacg attttctttc accactagat
     5881 tttcaataga caaaacgact tcttgaggct tcgcttcttt tttcatcgtt ttgaatgaaa
     5941 ccgttcttcc aaccatcata tcggctaatt gttgtgaggt tacgtcttta acgttgactg
     6001 taccgattcc ttttccacgg cggataactg tacagcggtc agctactgct ttgatttcat
     6061 ctaacttatg cgtaatcaaa atgatcgact tgccttcttt gactaattcc ttcatgatca
     6121 cgattaattc atcaatttct tgaggggtca atacagctgt cggctcatca aaaatcagta
     6181 catctgctcc tcggtaaagt gtttttaaaa tttctacccg ttgttccatt ccaactgaaa
     6241 tatcacgaac gtaagcatct gggttgactg acaatccata ctgttcagaa actttttgga
     6301 tctcttttcg tgctttttta tggtcaagca tccctgcgcg actaggctcg cttcccaaca
     6361 cgatgttttc tgttacagta aaggcatcaa caagcataaa gtgctgatgg accatcccaa
     6421 tccctaattg gttggctttg gtcggacttg tgatctttac ttctttccca cgcatcaaaa
     6481 ttttccctga tgtcggctct aataatccgg aaagcacgtt catcaatgta gatttgccag
     6541 caccgttttc tccaagcaac gcatgaattt ctcctgcctt tacttgtaaa ttgatgttgt
     6601 catttgcctt gaatgttcca aattctttgg taatattctg catttctatg acaatatttt
     6661 cttgaaccac ctttttttca catcctaacg atttctatct agggcaacga ttgactaaga
     6721 ttcacgctta gtgagcgttc ttcttttgaa acaaacatta cttcagggag ttgttccctt
     6781 atccaaactg tcacaaggca gcttggataa atgaacaatt cttgttctaa tcaaaccata
     6841 gccttgtcaa acatgtgtct aacaaggcta tagaaatggt tttagttttc ttcaggagtt
     6901 tctggaactt ttacgtcacc tgaaatgatt ttttcttttg cttcatcaac tgcttttttc
     6961 gcttcgtcag aaagttgtcc ttctgttaga ccaacaccgt cttctttcaa tccataaaca
     7021 gtgtgttcgc cgccagggaa tttaccgtcc gctgattttt gtgccaaatc ttctactact
     7081 gttccaactg ctttcagtgt agatgtcaat gtgaagtttt tcttttcgcc gtttaatgtg
     7141 tattcgcctt cgtctgattg gtcgcggtca acaccgataa cccaaacttt tttgtcgcct
     7201 gattcgttca atgattttgc ttcttggaat acaccgttac ctgtaccgcc tgaagcatgg
     7261 aagatgatat ctgcgttttg tgcatacata ccttgagcaa ttgaacgtcc tttatctgga
     7321 gcagagaagt caccagcata ttgattcaat actttgattt cttttcctaa ttctttcgca
     7381 cctgcatcaa caccggcttt gaatccagca tcaaaacgat cgattacttc accttttaca
     7441 ccgccgataa agccgactac gtttgtttca gtcgtataag ctgctgcaac acctgcaaga
     7501 taagaagctt cgttgtcttt gaaagttgca gatacaacgt tatctaatcc atcgatgaca
     7561 tcatcgataa taacaaagtt cgtacctgta ttgtttgttg cttgttcttg gatcgctggt
     7621 tttagtttat aaccaatacc aaaaatcgtt ttgaatccag cattcaatgc ttggtcaata
     7681 tttgggatgt aatctgattc atttgaagat tggaaatatt ggaagccatc atttcctcgt
     7741 gaaagtcctt gatctttccc ccatttttct agtccttccc aagctgactg gttgaatgaa
     7801 cggtcgtcta caccacctgt gtctgtgatc aatgctgcag ttgttgtgtc tgatgagctt
     7861 ttgccaccgg atgaatcagt agagccgttg ttgccgcatg cacctaaaaa taaccctgct
     7921 gctagtgcga cagcacctaa accaaataat tttgcttttt tcatttctgt aaagcctcct
     7981 aaaataattg ttttattttt caacagtcag ctgactgaat gaacatgagt tatagatctt
     8041 tttcagtaaa tgcataaggc agcagtgcac gaacggtcgt tgccttcgtc acaccattgt
     8101 ctcctactag cgtaaccggc atatcagggg aacagaattc tgccattact tgtcgacatg
     8161 caccacaagg agagatagga tcgggggtgt gccccgctac gactagatgc gtaaatgttt
     8221 tttctccttc agatactgct ttgaaaaaag ctgtgcgctc ggcacaattc gttaatccaa
     8281 atgatgcatt ctcaatgttg atgccttgat aaatcttgcc tgattccgtt acgagacaag
     8341 cgcctaccgg aaaatgggaa taaggaacat aggctttatc taacgcttca atagctgtgt
     8401 tgatccattc ttgtttgagt tctgccatga gcttctcctt tgaaaaaatt aatagccttc
     8461 accagttgaa ccagatacga ttgctactcc tgcgcttgtt ccgattcttg tagcaccagc
     8521 ttcgatcatt gccattgctt cttcttcgtt atggataccg ccggaagctt tgacacccat
     8581 ttctggtcct acagtttcac gcatcaaacg gacatcctct acctttgctc cgcctgttga
     8641 gaaaccagtt gatgttttga caaaatcagc gcctgcagct tttgctattt cgcaagctcg
     8701 gatgatttct tctctatcta ataacgatgt ttcaataata actttgacaa gcgcacgatc
     8761 ttttgctgct tcaacaactg cttcgatatc tttttggact tgttgatcat cctttgattt
     8821 caaagcaccg atattgatga ccatatcgac ttcatctgca ccgttattga tggcatctgt
     8881 tgtttcaaat gcttttgtct cgggtgtgtt tgctcctaga ggaaacccga ttactgtaca
     8941 tacggctact ggctcacctt gtaattgttc tttcgctagc gcaacccatg ctggattgat
     9001 gcagacagaa taaaaatggt atttttttgc ttcttcgata atttgcatca cagcttcttt
     9061 agatgcgtct gctttcaaaa tagtatggtc gatcattcgg tttagttcca tttcttttcc
     9121 ccttttcttt aaaacggtaa agtccgcttt tttcctttat tctgtgatga tgtcatgaat
     9181 caacgttggc ttcattgccg attcaccaat ttcaatattt ttatataata attcaatgac
     9241 agaagtgatt tcttcgtcgt tactatagat cgtcaatagt gactcacctt ttgtcacagt
     9301 gtcaccaatt tttttatgca gtttcaatcc aacagcgtga tcaatatctt cttctttggt
     9361 tttacgccca gcacctagca tcattgcagc aattcctaat tcatttgcca cgatttttga
     9421 cacaactccg ctttgtttag caggaagctc cattgtgtag cgtgcagtca aaattcgttc
     9481 tggatgttcc acgatcgctg ggtctccccc ttgattttgg atcatttctt tgaattttgc
     9541 taacgcttta cctgattcca gagcttcttg aagcaacgtt cttgcttcat cggttgtctt
     9601 agctttgcca gcaagaacaa ccatttggct tcctaaggcg tagcacattt ctactagatc
     9661 ttctggacca tttccttgta atgtttcgat tgcctcgaca acttctagac tatttccgat
     9721 agcttcgcca agaggttctg acatatctga gataaccgcc actgtttctc ggttagccag
     9781 tttcccaatg ctcgtcatgg tctttgccag acgtcttgca tcttcgatgt tcttcatgaa
     9841 agcaccgtct ccagtggtca catccaaaac gattgcatct gctcctgccg cgattttttt
     9901 actcataatc gaactggcaa tcagcggaag agaatcaacg gtcgctgtga cgtcacgcaa
     9961 ggcatacaat tttttgtccg ccggtgccaa gtttcctgat tgaccaatta ccgctacttt
    10021 actttcatta acaatgcgta caaattcttc atccgaaagc tcgatctgaa atccgggaat
    10081 tgcttctagc ttgtctagtg taccacctgt gtaacctaga ccgcgtccag acattttagc
    10141 cactgtcact ccgacactag ctactagggg ggctaaaatc aatgtggtcg tgtcaccaac
    10201 gccacctgtc gaatgcttat ctacctttat tccttcaaga gaactcaaat cgatgacatc
    10261 accagaatta gcaatcgcca atgttaagtt cgtgatttct tcatttgtca tgtcttgata
    10321 aaaaattgcc atcaataatg cactgacttg ataatctgga atctttccat tcgtatagtt
    10381 cgtgacgatg tactcgattt cctctttcga cagctcgttt ccatccctct ttttttcaat
    10441 cagatccacc attcgcattg cctgatcctc cttgtcttca ttcggtatta tacattattt
    10501 ttaggaaagg taaaccactt tagtaaactg gtttactaaa aataaaaaaa gcactaacta
    10561 gcctttgtaa atgcttacat aagcaatata ccgccgagtt agccctttgt caatactttt
    10621 acgctctcac gaataattaa ttttgtttcg aacactttgt tcggaacttt tctttgagga
    10681 aattcgatcg tatcgatcag gaaacgtgca gcataaaatc caatatcaaa acttggctgc
    10741 tgcactgtcg tcaattgcgg aatcatgtat tctgacagct ttaatccatc gaatcctatg
    10801 actgagatat cttctgggat ttttttgccc aattcatcga tcgcctgata gcatccgatc
    10861 gccatggcat cattcccgca aaaaatagcc gtgatatctt tatgtttgag taattttttt
    10921 gatgcagtat atccaccttg gacagtcaac tctccatgtt caacaaattt cccattaaaa
    10981 ggcaggccat gatctttcag cgcatgacga tatccgttga aacgttcttc taattgatag
    11041 tatccagtgg tttccttcag catgccgatc ttcgtatggc cattttcaat aaacagcgaa
    11101 acagcttgat aggcgccctc atattccttt acgataagcc gtccgttatc ccgacgattc
    11161 aatccgcgat cgattaaaat gattggcatt ttttgataaa aaccgctgtc taatgcatat
    11221 ttctcaggta aaatatttgg tgtggccagc aagattccgt ccactgagcg gtgggaaagc
    11281 tcttgtaaat acttgatctc attttcttgg ctatgacgag aattgcataa catgatcaca
    11341 tagcccaaag gattcaaata gctctctaca ccttcgatca gtttggagaa gaagaaatca
    11401 gtgacatcag ggacgatcat tccgatggtc tttgaatgtc gattgatcac atttgaagcg
    11461 aaataatcgg gcttataatg atgttcattc acgatcgaca acactttttt tcttgtctta
    11521 tcactaaatc tgcttccttt gttattcaaa atctgtgaga cagttgttac tgatacgcct
    11581 gccatttctg cgatatctct gatggtcagc ttcatcctat tggctccctt ctatctaaat
    11641 tccctatcag tgtaccaaaa cccccaagaa aagaatagag aaaaaaggaa agtatgtttt
    11701 ttgataaaaa cctcctttat ctaccgactt ggcagtcttc tatacaagtt ctgtatctta
    11761 tttattttat cgtaaaaaac aacgactctg ttcctactat ttctttttga agaaaaaata
    11821 gttctttgtt tttaaaaact tctcaacaaa aaaagaactg atacgtaccc gtacatgcac
    11881 gcccagttcc actctttcgt cgctccacta tttttcagct agttattctt tcacactttt
    11941 gatgatataa tatcctttat cttttgtgac gatttctgca ttaccaaatg tttcttccat
    12001 tttcttttgc gcactcggcg ctccttgttt cttctggatc acaattgtca gtgtaccgcc
    12061 cttcttcaac cgaggatatg cgcctgtcaa aatttcatgt acaacttttt ttccagcacg
    12121 gatcggcgga ttgcttacga tagctgcata ggtttcttca tgaactgctt catagatatt
    12181 cgattgatgg atatcaacat gttcgatttg gttacgcttt gcgtttcctt gcgctagact
    12241 taccgctcgt tcattaatgt cgaccatttc aacaaaccgc tgtgtcgcaa atgccaaagc
    12301 taacccaatc ggtccatacc cgcatcccac atctaagatc tttccttcat caggtaactc
    12361 ttcccaatcg aaagcatcaa tcaggacacg tgaaccataa tccactgttt cacgtgaaaa
    12421 tacaccagaa tctgttacaa attgaaaggt tctgcctttt aactcaaaag cccattgttc
    12481 aaaatcgtgc gccgtatctg ggcgttcaga atagtaatga tttgtcatgt cttccatcct
    12541 tctcatttct ttcatgacaa cagtctatcc ttttatgtat ccgtgcgcaa gtcaagaata
    12601 agactccgtt tctttctatt ataaagatta tacaaaaaga tcggcaatct ctcttaagtg
    12661 gtggggtttg cgtttatttt gtgttaaaat agttccattg atgggagaac gcaggaggct
    12721 aatatgaatg atccaatgaa ctactaccgt gtggcaagag aagaatggcg agaattttat
    12781 cgtaatgggc aagcaccttt gacacaagat gaactagata atatcaaaag tttgaatgac
    12841 cgcatttcca tgcaggatgt tcgagatatc tatgtacctc ttgctcatct gatttatctt
    12901 tatatgaaag aatttgaatc tttgttcttg agtaaaggtt tatttcttca taaatacgta
    12961 gaagtaccgc cttttattat cgggatcgcc ggaagtgtag ccgttggaaa aagtacaaca
    13021 gccagactgc tgcagacgat tttgtctcgt accttcaaac gaagaaacgt tcagctgatc
    13081 acgactgacg gatttcttta tccaaatcgt atattaaaag agcgtggaat catggaccgt
    13141 aaaggatttc ctgaaagtta tgatatggag atgctgatcg atttcttgaa ccgtgtaaaa
    13201 tctggtcagg aagagataaa agtacctgtt tactcacatg atgtgtatga tatcattcca
    13261 gacaagtacg aattgatcca gcagcctgat atcttgatcg ttgaggggat caatacgctt
    13321 caattacctg cgaatcagca aatctacgtc agcgactttt ttgatttttc tgtctatgtc
    13381 gatgccgatc ccgtgctgat cgaaaaatgg tatttggatc gttttggtgc gttactggat
    13441 acggctttcc aaaatccaga gaattattat tatcgttttg ctatcggaga tcgtgaagaa
    13501 gcctttaaaa tggcaaaaga tgtatggaaa aatgtgaatt tgaaaaattt acaggaatat
    13561 atcttgccta cacgaggaag agccgatata attctccata aaactgaaaa tcatatcatc
    13621 gattcgattt tcatgcgaaa atattagcat tttaaaatag tttgactatt ttgagataaa
    13681 atttaaaggg ataggggtaa taaatttgac aaacgttgcc gatatgacga acgttgaaaa
    13741 gattatcgta ttagactacg gtagtcagta taaccaattg atcacaagac gtattcgtga
    13801 tcttggagtc ttttccgaat tattgagcca ccgcattact gcagaagaag taaaagcgat
    13861 gaatccaaag ggaatcattc tttctggcgg accaaacagt gtatatgatg agaatgcatt
    13921 tgggatcgat cctgagatct tcaatttagg tattcctgta ttagggattt gctatggtat
    13981 gcaattgatc acgtacaaac ttggcggttc cgtagagcct gctaaaaacc gcgaatatgg
    14041 gaaagccttg ttagaagtaa caagcgatca agctgtcttg tttaaagaaa caccaaaaca
    14101 acaaacagta tggatgagcc atggtgactt ggtaactaaa gttcctgaag gctttgaaac
    14161 agtcggaaca agcaaagatt gtccgattgc ttctatccaa gacacaacac gtaattttta
    14221 tggtatccaa ttccatgcag aagtacgtca ttccgaatac ggaatcgaat tgctgcgcca
    14281 ctttgccttt gatgtttgtc actgtgaagg taactggaca atggataact ttatcgatat
    14341 gcaagtagaa aaaatccgtg aacaagttgg tgacaaaaaa gtcttgcttg gtctttcagg
    14401 tggcgttgat tctagtgtcg ttggggttct tttacaaaaa gcgatcggtg accaattgac
    14461 atctatcttt gttgaccacg gtctgctacg caaaggcgaa gcggaacaag taatggaaag
    14521 tctaggcgga aaattcggct tgaacatcat caaagtcaat gcaagagatc gcttcttaga
    14581 caagttgaaa ggtgtatctg acccagaaca aaaacgtaaa atcattggga atgaattcgt
    14641 atatgttttt gatgacgaag caacaaaact tgctggtgaa gaaggcgtct ctttcctagc
    14701 acaaggaacg ttgtatactg acgttatcga atctggtaca gaaacagcac agacgatcaa
    14761 atctcaccat aatgtaggtg gactgccaga agatatgcaa tttgaattga tcgaaccatt
    14821 aaacacatta ttcaaagacg aagtccgtga attagggact cagttaggca tgcctgattc
    14881 aatcgtttgg cgtcagccat tccctggtcc tggtcttggt attcgtgttc ttggcgaaat
    14941 cactgaagac aagctagaaa tcgtacgcga atctgatgct atcttgcgtg aagaaatcgc
    15001 agctgctgga cttgaccgtg atatctggca atatttcact gttcttccag ggatccgctc
    15061 tgtcggtgtt atgggtgacg gacgtactta cgactacaca gtcggtatcc gtgctgtaac
    15121 ttcaatcgac ggaatgaccg ctgactttgc ccgtatccca tgggaagtct tgcaaaaaat
    15181 ctctgtacgt atcgtaaacg aagtagccca cgtcaaccgt atcgtgtatg acatcacttc
    15241 taaaccacca gcaacagtgg agtgggaata aagagccaac ataatatgga gggggaatcc
    15301 ctataaatca acattcttca atagctaaaa tgcttgattt tcattaaaaa taagatggtt
    15361 ccctaccaaa agagactata atataagtta atttgtttca ttcttatcat aaaaagaagt
    15421 tctcgaaaaa cgaactgttt tccgagaact tttttctatc catctaagtg ctcaaatcat
    15481 attcacgacg gaattctaag gttacccttt cttaaacaaa ctttatgtta tctgcacaac
    15541 tttttgcaat tttttcattg cttcttacga aatcgttgaa ctctctttag cataatacaa
    15601 agtgtagatg aagtctggtg ctttactcgt tttggtaaaa atgactggaa atcgtttctg
    15661 gttatttttg gcaaaggcga ggtgcaggtt cgattcctgc cttctacgct aacgatgtgg
    15721 ctgaagggaa cctccgatct tgaatcgaaa cgatccgcag tctattgttt caccaatgaa
    15781 acaaaatcaa cgttcgtcca agcatttcct gaagccttgc tataaacccc aatttcacaa
    15841 acacctgttg taattggaat gtctttgatt gtaatttttg tccattcact tgttttaaca
    15901 accgcagtat ttagttccgc tccaccataa tttttgatat ataactgatt agtatcaaac
    15961 tctcccccag acattacata ggcactcaat gtgtaagttc ctttgcttat gccactaaca
    16021 ttttgataag ctgatgcctc gtatgcttga tcctgccaaa aagttagttt gtagtcccca
    16081 aaaaaagtgc ccgcttccgt tttgatcgta cccttagcgg ttccttgttg aaaccaggtt
    16141 tgccaattgg aaggactggt cgttggtcca tcgctttcga aacttgcatt tttaactaga
    16201 tttatttgcg cccctgcttg tttaaacgca cgcaagcttg gcaaggcgtt tccagcaaaa
    16261 tcaaacatcg cttggttgtc ccattcgttt cccatagtgg attgaacact gagataattc
    16321 atacctgctt gtgttgccca actcacattt ccgttgtacc aaagaggttc ccaatagaag
    16381 aagcccgccc cgcgattatt cggtactgcc tgaatccgat cggcaagatc cagcaaatac
    16441 tctgcttgtc ctgtcggtgt tggttcatat ccccccgcag ctgcttcggc ttcaccaaaa
    16501 gcatttggca tagtatcggc attcgctgtt gtatgcccat aagctgtttc aactacgatc
    16561 acttctttac cataccgttg gctgatcgca ttcatattgg tttgaagttc attcattgaa
    16621 ccgtgccaat acggataata cgacactcca attaaatcaa atgacacacc tcgactcgtg
    16681 atttcatcaa accaccaagt aaatgctcct gtatcgccac catcagctaa gtggagcatt
    16741 actggagttg ttttaggttg agtgtctcga acagcttgga taccagactt caaaaaagca
    16801 gccaatcgat caaattctcc cccgccttct ccccaagact tgccataagg ccagagcatt
    16861 ccgctattca gttcattgcc gatctgaacc atatcaggat aaacgtccag ctgcttcatt
    16921 tccgacagaa cgttctttgt atagtcgtac aatgccgtat tcatttcttc aaaggtcaga
    16981 ccttgccatg ctttaggaag attctgtttc ccaggatcaa cccaaaaatc actgtagtga
    17041 aaatccagca gcaccttaag tcccagattt tttgctcgtt ttgcaaggtc aatcgtcgtt
    17101 tccagatcat ttgtccctgc tccataactt tctccattac tattataagg atcctgccac
    17161 aaacgcagtc gaacatagtt gacaccatta ttttttaaaa tctctaaggc atcttttgaa
    17221 atatcgtctt cataatattt tgcaccagat ttttccatat ctgacaggat cgaaatatct
    17281 gctccgcgaa taaattcgct ggcagatgct tcaacagttt ttgaaacagc ccccaaagcc
    17341 cctaacacag ctgcaaataa aacagcttta cgcaaccaaa ttcccatttt cattatggct
    17401 cctcctttat tgaaattggt tcagaataaa ctgaacagct ttttcaggat ctctcgttaa
    17461 tccgatatat tctgctcctt ttgtcgcaac cagcttgatt cctaagcgat cggtgtcttc
    17521 tttgatatca tcatagtaag aattgacttg cggtgctaag accaccatat caaaatcctg
    17581 cagcatttcg taatgagaac cataagcacc cgccattgca ctgatcggtg ctttcgattc
    17641 agatgcacct ttttttaaag cattagctaa catcgcactt gtccctgcgc cagcacaaag
    17701 taccaacaca ttgatttctt tatttgcatc gctgataact tgactcgctg ccacaggtac
    17761 ttcctcacgt acatcggcag aagcaccttc ctcggttgcg atcacctctg ctttttgtgc
    17821 ttctttctct actaagacag catcgtatgc tttgcaaaat ggtagataga tcaggaagtc
    17881 aacgacgatt agaaccgctg ctagaacaaa agctaagata cccatgcccg ttcctagaac
    17941 caaaccgatc ggtgctggag ttgcccaagg caaaacgtac ataaagctgt tcatgcccaa
    18001 tacatcgaca aaaaacttga acaggcaaac gtttacaaca ggtgttaaca aaaatggaat
    18061 aaaaaaataa gggttcaaaa tcatgggtgc cgcaaatagc aatggttcgt tgacagcgaa
    18121 acttactgga ataaaggaag ctttcccaac tgccttcaac tgcttagact tagcaaagag
    18181 taggaagata aaaggaacaa ccaatgttgc cccagttcct cccatagttc ctacaaaatt
    18241 ccctaaaccc accgtcagta ctttagaagc ttgttcaccg gaattgaaca attgcagatt
    18301 ggtttccaca ttggcataga taattgccgc aattgccggc tccacgatcg acggtccatg
    18361 aacaccgaca aaccaaaaca gcgccatcgc tccccagata atagcgattc ctaagtaccc
    18421 gtcagctgct gtaaatagcg gctgcagcac cgtaatgatt gcttctgcaa atgagatttg
    18481 gaagatttgt cgaatgccta aatcaattaa aacggccgca aatactgaaa aagagaaagg
    18541 aaaaatatct cggaaggttt gcgaaatcgt tccaggaact tctttaggca ttttaatggt
    18601 aatatctttc agcacacaaa acttatataa attaactgtt aaaaatgcag cgacaaagga
    18661 agccagtagt ccctttgtcc ccatatattc cgttgaaaat ccaccttcaa tcggagagac
    18721 gcttaacaac aggaaactta ccacagaggc cagcatcaca gaggtggtgt tgattacttt
    18781 gtttttcggt aagcgacgat tcatcgaatc agccaagcag cgagcagtgg ttgctgaaac
    18841 gagtaaccct acaacaccca tcgaataatt atagactttc catagccatg tggagacaga
    18901 ttcaggccat tggtaaccaa aaatctcagg aaccgcagca atcaaaatga aaatgcttga
    18961 aaataaaata gctggcatcg ctgttaaaaa tccgtcacga acggctccta aataactatt
    19021 acgtgagatt ttttcaaaaa atggtttccc tttttcgatt tgtttaatga ttccatccat
    19081 agcgttcttc tcctatcctt gattctgtct gtacagtaca ataaagtctt gaatcagatc
    19141 tctcagcaat aaagtggtca ttaaatgatc ctgcccatgg ataaaaatga atcccatctc
    19201 catctctttc ccagatgctt cttccgctaa aatttttgtt tgcgaattat gtgcaagggt
    19261 taaattttcg tcggcttctt tcaacgccga atcaacatgt tcaaagtttc cttgtcttac
    19321 ttctttcaat aatttcaata atgtactgcg agcgtcgcct gaatatgcaa cgatctcaaa
    19381 accaagcatt tgtaattcct ctttattcat cttttcttcc tccattatca cttattaaat
    19441 aatcgttttg gtttcactga ccaacttgta ccagtaagct gattcttttg ggtaacgctt
    19501 ctgggtttca aaatcaacat aaaacaagcc ataccttttg ttatacccat ttgtccatga
    19561 gaaaagatcc ataagcgacc acaaaaaata ccccttcaca tttaccccgg ctgttatcgc
    19621 cttgcttaag gattccaaat agactctcaa atagtcgatt ctcggctgat ccataataat
    19681 gccatcttca aactgatctt tataccccat cccgttctca gtgatataga ttttgttgta
    19741 atgtgggtaa tcgcttttaa ttcgcagcaa caagtcatat agaccttctg gataaatcag
    19801 ccagtcccaa tcagttcgag gaattccttc tttataaata cgttctccga ttcccttcac
    19861 tttataaaca gaggtccctt tttcacccgt accgttatga tggattgcat tttctccatc
    19921 gtaagctttg acaaaatggc attggtagtg attgatccct aagtagtcat tacgcgtgga
    19981 tgcttttttc aattcaacga aatcttcttc aggaaaatga tagctggcct gatttgcttc
    20041 acatatctca tcaagagcag tcaaggtttc agttgaataa tagcctaaat aggtagcatc
    20101 taataagaaa cggatcgaca atgcatcgtc taaaaaggca gcatgcttat cttccggcgc
    20161 gtctgttgcc gcatatttcg tctctaatga gtgaactaca ccaatttcac ccggaagttc
    20221 gttttctttg aaatagttca ctacacgggc atgagcaacc atcatattat gaagacaggc
    20281 gacgatcttt gtaaaatcat atttgattcc tggtgggaaa acacctaaca aatattgatt
    20341 ggttgcaact ggatagattt cattaaaggt actccacacc ttgacttctt tgaattcatg
    20401 aaaacaaaaa atggcatagg aaacaaatgc ttctattgtt tctcgattca aaaaatctcc
    20461 atggtcaaac aaacgtttag gggtatcaaa atgatgcaac gtcacaaacg gcgttacgtt
    20521 tcttttctta cattcttcga acacacgatg ataaaaagcg attccttctg gatttggttt
    20581 cccagctcca tcaggaaaaa tgcggctcca agcaatggat aatcttagac catttacacc
    20641 aaaacgttca cacaattcaa tgtcctttgc atattgatga taaaaatcac ttgccggatc
    20701 gggactgaat cgcccttgtt cctctaaaaa gtcatcccaa gcaacagcgc ctttgccacc
    20761 ttccttagtg gctccttcaa cctgataggc tgctgtggct ccgccaaaaa taaaatcttc
    20821 tggtaattta agcatgtaca tctctcctca aatctataat cagttttcgc tctttctaac
    20881 tgattgcatt gtaaacgcat ccaaaacatt tgtcaacaaa aacaaacaca aataatcata
    20941 aaaaccagaa aacagcaata aaatctattg taacaaacat aaaaaccacc ctaaatgttt
    21001 acttttgttc gaattagatt gactgttgtc cgtttatatg atagattatc cttaaattag
    21061 aggtgaagac aatgttgaaa aaagaaagat tgcttactat cgtagaaatg gtcaataaaa
    21121 aaggaatcct taccgttaat gaaatcatca atcaattaga tgtttctgat atgaccgttc
    21181 gcagagattt agatgaatta gaaaaatcgg gcaagctgct gcgtgtccat ggaggggctc
    21241 aaagtctttc ctacacttta gatcaagaac tttctcacac tgaaaaagcc actctgcaga
    21301 ttcaagaaaa aaaagaaatt gctgccacag cagcctcctt gattcacgag ggagaaacga
    21361 ttttcttagg accaggtaca acaatcgaac tattggcgac gcatttactg aacaagaaaa
    21421 ttcggatcat tacaaataat tatccagttt ttgatattct tgctcaagca gatggaccag
    21481 aaattctact gattggcggt gatttccgga aaaataccgg cgcatttgtc ggaccgatca
    21541 ccaacgacaa tttaagaaga ctaaagttta caaaagcatt tatcagttca aatggcgttc
    21601 acaacgaagc gatctctact tacagtaccg aagaaggaga agcacagcag atcgctctaa
    21661 ataattcccg taagaaatat ctactcgttg ataataaaaa attcaatcga gatgattttt
    21721 atgtgtttta taatttgcat gatttcgatc tgctcattac ggatcgccag atctcgcggg
    21781 aggttcaaga gcactatgca caatatgtcg atctgcaaat tgctgattca accgaaaata
    21841 ccactatagg agcgtaacta tgaaagggat tatatttgat ttcaacggaa caatgttttt
    21901 ggactctcat ctgcatgaag ctgcttggct gcatatgata catgatcata caaaagatgg
    21961 tttatcagac gaggatattt taaaaaatat tcatggtcgg acgaatacgg agattttgaa
    22021 acatttcatt tcagagcatt tgaccgcctc tgagatcgcc gtattgtcag aagaaaaaga
    22081 aagctattat cgtatgcttt gcttgcaaaa tgaaaacgag cttcagttga caaaagggct
    22141 tgaagcgact cttgaccagc tgactgacca acagattcca ttaacgattg ccacggctac
    22201 tgtcaaagag aatgttgctt tttattttga tattttcgat ttagcccgct ggtttgactg
    22261 ggacaagatt gtctatgacg acggctcttt ccctgggaaa ccgcaaccgg atatttttct
    22321 taaagcggca gacaaactga gcttagctcc ggctgattgt cttgttatcg aagatgctta
    22381 ctcaggatta cttgctgcca aacgtgcggg gatcggaaca attattgcaa ttgatccatt
    22441 cggtaaaaac aaaacagtat ttgaacaggc acagctaaat aaagatggaa ccatcaaaga
    22501 ttttactggt ttttggacag agcacctgtc tgccacatcc gttaattctt aaacgattca
    22561 actaactacc aaagaaaagc aactgaaatg cacacctcct tttataaaaa actgccagtg
    22621 aaacgacatg aaagatacac tgctatatcc ttagtgaaat atagcagtgt atctttgact
    22681 ttttcattct tttaaccata tgacgcgttc atctctatct ataattaaca ttaaaaatag
    22741 tgttttcaca ttatttctca aaccaatctt aaaaatcatc aggagtactt ttttgatata
    22801 cttttattaa tgctgggcgt aatggttaaa agatttccac tagttcctct ccaccaagct
    22861 gagttgaaaa atgcttatct gtaaacaaca tagacaaaga ggaaataaac gtttccgtat
    22921 ctttctctgt cccaaggata ttaattaatt ttcctactag gtaagtatca ttaaacttaa
    22981 gatctgcctt cacatcaaaa gttttgataa aagcttttat catgcgtaca attttgtctt
    23041 tatcgccata attgatttca ccactcatat caatatagtc actaatccct gaagaaataa
    23101 tggcaactgc ttcttctttc gccttatcag ataattcatt taatgactgg gattgattga
    23161 tgacttcttg agttttatct gttacgtaat ctctcattat cctatcggca tactccgcaa
    23221 agtaatcttg aaaaaattct tctacagggt aaccaacttc gttttcccaa ccaggagaaa
    23281 tttcattatc agtacttatt aaatctatta aaaaccaacc cgttctctga gtgttgattc
    23341 tataatattt ttaatttcct atataaatca aaaatattat agaatcaata agctaattaa
    23401 gaaacaaact ttagaaactc tagaagaact tctgaattta tatagaagtg tgtagctact
    23461 tactaaatat acaaaagtta aaataaaata ctctggatac tctttatact gcattaaagt
    23521 tagtaatatt aacagcaaag atatcgaaaa aacaataaca taatatgtgt caaaattttt
    23581 cataataaga actcccttac tgttgcatac taacataatc caaaaactct tctacttcag
    23641 ctaaagacat tccactttct ttagtccaaa aaacagtttt ttctatcgat ccttctctgt
    23701 aaactgtaaa tgtcggagtc gagttaattg agtagcgact cttgaattct ttccactcat
    23761 tttcatcttc tctaactttt gctacattca cgtaataaat attttctttt cccttttcta
    23821 ccagcatatc gaatagcttc ctgcttttga tgatcgattt cttgtgctac atctagtaaa
    23881 ttttcatcaa tttctatatc ataaagtatt tcactagtgt ctacatccac aatatatact
    23941 ttttatcagt agcaattttt gagtaatccg ttaaaatgat ctcagtctcg tcactttcat
    24001 cagtataaat atacgttaac aatgaactca ccttttcaat cggtgttagc tctaagccta
    24061 ctgtattttt tactattgag tcattattta ccaccataac ttcgtactgt atataccatc
    24121 ctatttggtt tccatcaata tcagtaattg gctcactagc taatggagtc tccggaacaa
    24181 tattttcctg atatttctta aactcttctg tagcacgaaa aaggttatca tgttcctcac
    24241 ttgaaactgg ttgagtcata gaaaatgtta cttggctaaa cagagggaaa ataaacacta
    24301 caatcaacaa agtataaaaa aaagcccttc tttttcattt tttatcttcc aatcacaaat
    24361 tgaaaaactt attcctgcaa ctttgagtct agtatcacaa tcgactgact acaactattc
    24421 atttacattc tactttaagt tcctccttaa ccattaattt tattaaagat acttgtaata
    24481 gtgattctca caaaatttca tgaaaaatac tttattactg tatctaaaat tccaatacac
    24541 aacttatcga ctattatctg aaaaccaaaa cttattattc cttgatgtta ttctagcaat
    24601 agtaactgct tttttatttt tttgcatatt ttttcggcgt tactttttaa agttaacttt
    24661 ttcgacaaat agaaagagat acgaatcact gatttttttg aagaaaagaa atggagtgag
    24721 ctgaacgtta gagatattgg gagctttcgt aactgcttat taaacgataa tggttatttg
    24781 caaccgtatt gctcgcttct acatgaaata tttcatgaaa tatttcgcaa aacctttgat
    24841 tatgtagtta cgtttccatt cttgactaaa aatgtttcaa aaacgaacaa aggtgttaaa
    24901 aggtaaagtg atcaatacta ctggtcaaaa taagaggttg aaagggaatt gaaaaataac
    24961 tacagataat acttttataa acgtacgtgc tttgtggttc tgtagttaac tatataactg
    25021 gaattaggga atggtatcaa tttgaaaaga aagattctac caatttatca tatgttaaaa
    25081 atggaaaatc aacagcagtt ctttcggaaa ccttacacaa aaaccgatta ttgaaaatag
    25141 ccgaaaaacg tttttcggct atttttttaa ttaagtttcc ttcttcttac cttttcccac
    25201 tattccgttt cacatctttg atgatcttca gcgcacgttt tcgtgttttg atatttgggc
    25261 ttttcaagcg gcggtatgca cttgagatat ctgttttttc catatggacg acccactttc
    25321 ttctttgatt gtagtcatgc taaaaaacac aaatcgaaac aattacgatt tgtgttttaa
    25381 atggtatcaa aagttaaaat gaaagtcaac ttgttaaaac agtcgatcag actagctgat
    25441 tgactcctat ctctggaaat aaattttcat tattacgttt tagctaccta aaaaaggccc
    25501 tcttgtccgt taactttaat atctattgtt atagctagtt tacctattga acgtactgtg
    25561 tgttcgatag aacac
//
LOCUS       DAZUMI010000031        24390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000031 DAZUMI010000000
VERSION     DAZUMI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.031"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..858)
                     /locus_tag="ACG81C_001090"
     CDS             complement(10..858)
                     /locus_tag="ACG81C_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5064267.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1082..3022)
                     /gene="thrS"
                     /locus_tag="ACG81C_001091"
     CDS             complement(1082..3022)
                     /gene="thrS"
                     /locus_tag="ACG81C_001091"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5064268.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3359..3538)
                     /locus_tag="ACG81C_001092"
     CDS             complement(3359..3538)
                     /locus_tag="ACG81C_001092"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5064269.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3585..5408)
                     /locus_tag="ACG81C_001093"
     CDS             complement(3585..5408)
                     /locus_tag="ACG81C_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5064270.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5686..7965
                     /gene="helD"
                     /locus_tag="ACG81C_001094"
     CDS             5686..7965
                     /gene="helD"
                     /locus_tag="ACG81C_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5064271.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8058..8705)
                     /locus_tag="ACG81C_001095"
     CDS             complement(8058..8705)
                     /locus_tag="ACG81C_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064272.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8884..9603)
                     /locus_tag="ACG81C_001096"
     CDS             complement(8884..9603)
                     /locus_tag="ACG81C_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5064273.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9734..10885)
                     /locus_tag="ACG81C_001097"
     CDS             complement(9734..10885)
                     /locus_tag="ACG81C_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5064274.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11102..11842)
                     /locus_tag="ACG81C_001098"
     CDS             complement(11102..11842)
                     /locus_tag="ACG81C_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5064275.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11846..12169)
                     /gene="rsfS"
                     /locus_tag="ACG81C_001099"
     CDS             complement(11846..12169)
                     /gene="rsfS"
                     /locus_tag="ACG81C_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5064276.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12226..12819)
                     /gene="yqeK"
                     /locus_tag="ACG81C_001100"
     CDS             complement(12226..12819)
                     /gene="yqeK"
                     /locus_tag="ACG81C_001100"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5064277.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12803..13453)
                     /locus_tag="ACG81C_001101"
     CDS             complement(12803..13453)
                     /locus_tag="ACG81C_001101"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5064278.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13463..13774)
                     /gene="yhbY"
                     /locus_tag="ACG81C_001102"
     CDS             complement(13463..13774)
                     /gene="yhbY"
                     /locus_tag="ACG81C_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5064279.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13790..14899)
                     /gene="yqeH"
                     /locus_tag="ACG81C_001103"
     CDS             complement(13790..14899)
                     /gene="yqeH"
                     /locus_tag="ACG81C_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5064280.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14896..15423)
                     /locus_tag="ACG81C_001104"
     CDS             complement(14896..15423)
                     /locus_tag="ACG81C_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5064281.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15603..16388)
                     /locus_tag="ACG81C_001105"
     CDS             complement(15603..16388)
                     /locus_tag="ACG81C_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5064282.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16381..17250)
                     /gene="accD"
                     /locus_tag="ACG81C_001106"
     CDS             complement(16381..17250)
                     /gene="accD"
                     /locus_tag="ACG81C_001106"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5064283.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17251..18630)
                     /locus_tag="ACG81C_001107"
     CDS             complement(17251..18630)
                     /locus_tag="ACG81C_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5064284.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18638..19057)
                     /gene="fabZ"
                     /locus_tag="ACG81C_001108"
     CDS             complement(18638..19057)
                     /gene="fabZ"
                     /locus_tag="ACG81C_001108"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5064285.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19082..19558)
                     /gene="accB"
                     /locus_tag="ACG81C_001109"
     CDS             complement(19082..19558)
                     /gene="accB"
                     /locus_tag="ACG81C_001109"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5064286.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19561..20796)
                     /gene="fabF"
                     /locus_tag="ACG81C_001110"
     CDS             complement(19561..20796)
                     /gene="fabF"
                     /locus_tag="ACG81C_001110"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5064287.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20815..21552)
                     /gene="fabG"
                     /locus_tag="ACG81C_001111"
     CDS             complement(20815..21552)
                     /gene="fabG"
                     /locus_tag="ACG81C_001111"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5064288.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21564..22481)
                     /gene="fabD"
                     /locus_tag="ACG81C_001112"
     CDS             complement(21564..22481)
                     /gene="fabD"
                     /locus_tag="ACG81C_001112"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5064289.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22588..22812)
                     /locus_tag="ACG81C_001113"
     CDS             complement(22588..22812)
                     /locus_tag="ACG81C_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5064290.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22865..23827)
                     /locus_tag="ACG81C_001114"
     CDS             complement(22865..23827)
                     /locus_tag="ACG81C_001114"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5064291.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23837..24277)
                     /locus_tag="ACG81C_001115"
     CDS             complement(23837..24277)
                     /locus_tag="ACG81C_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5064292.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6667 a         5432 c         4249 g         8042 t
ORIGIN      
        1 gtaaggtttt tatttcgaat ccataaaata ttcttggatt gcttctcgtg tttttttcac
       61 tttttcgcta agcttttcgt ttttagccat atagaaataa aaaatgatat cgatcgtagc
      121 aaattgggcc aaaagtgaat tagttgccgc acttcgattc gtgatttctt ttggtctaga
      181 tgtttgtacg agtacatcgg ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc
      241 aatgatttcg atattcatgc ttttcgccag ctcggctaat gcgactacat ctgcacttct
      301 acctgagttt gaaacaagcc aaagcacttt ctttttctca ttgctgacaa gttgcgggag
      361 caagacgtgg atatcttttt caaaaatgat tggcttaccg acacgagacc atttttgcag
      421 aatatcttct actactaaag aagaggcgcc aaccccatat aaaaaaacag tctctgccga
      481 ttccaaacac tctacgactt tttggatcgt ttcttcttct agaatatcta ctgtttcttt
      541 tatcgctact tgtgcattgt taccaagttt gtttttgata gagtaaaagg actcatttgc
      601 ttctatatca ctaaaacctt catcgattcc agtctgtagt tctgttgaaa tcgtgatttt
      661 gaagtccgtc agactttgaa aaccgatttt tttggaaaaa cgtacaactg tcggcgcact
      721 caacccggaa gcttctgcta attcattagc tgtcattgta gggaccttct caatattacc
      781 gataatgtag ttggccaact ttttttctgc tgaagaataa tcttccatca tactttcaat
      841 acgactaatt acgctcataa gttcctccac ccttttaatg atagtctatg taagtatacc
      901 atatcagacc cttattcacg gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa
      961 cagaatatct tatcccatca atcccacaga ttctattgag catctgtgtc tttgaggaat
     1021 acctgttatc tattgttcgg attgctttct atctactact tttttacagg aaatttacct
     1081 attagttttc tctgctgtaa tttttgactt ctgcttccat agcatcaata aagatattca
     1141 aatcttctac agctgtttct ttacttccat aacgacgtac gttcactgtt gcatcttcca
     1201 attccttgtc tccaacaact aattggtaag ggatcttttg tgtttgagag gcacgtatct
     1261 tatagcccat tttttcatta cgatcatcaa cttctacacg caaccccttc atttgcaaac
     1321 gttctttgat ttcataagca taatcgccat gtgcatcaac agaaacaggg atgattgttg
     1381 cttgtatagg tgccaaccat gttggaaaag cgcctttgta tacttctgtt aaataagcga
     1441 caaaacgttc cattgtagaa acaattccgc ggtggataac tactggacga tgcgtatttt
     1501 ccccgtcttc tccaacatat gtcaaatcaa agcgctctgg caataagaag tctaattgta
     1561 tagttgataa tgtttcttct gtaccaagag ctgttttgac ttgaacatct agttttggtc
     1621 cgtaaaaggc tgcctcgcct tctgcttcaa agtaatctag ttctaattca tccatagcgg
     1681 ctttcagcat tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat
     1741 tgtttggatc gcgatagctc aagcggaaac gataatctgt aatattgaag tcagcatata
     1801 ctgccaccat caattcaagc gtacgtttaa attcatcttt gatttgatca ggacgaacaa
     1861 atgtatgacc atcattcaac gtcatttctc gtacacgttg taaacctgac aaagcacctg
     1921 atttttcata gcgatgcatc attcctaatt cagcgatacg aattggtaat tcacgataag
     1981 aatggatcgt gtttttgtag accatcatgt gatgagggca gttcattggg cgaagaacaa
     2041 gcatttcgcc atctcccata tccattggcg ggaacatgtc ttcttgataa tgatcccagt
     2101 gtccagatgt tttgtacaac tcaacatctc ccattattgg tgtatataca tgttggtaac
     2161 ctagactaac ttctttgtct acgatataac gttcgatcgt gcgtcggatt gttgcccctt
     2221 ttggaagcca gaatggcaaa ccagaaccta cttctgggga aaccatgaac aagtcaagtt
     2281 cttttcctag tttacgatgg tctctttctt ttgcttcttc acggagacgg atatattctt
     2341 tcaaagcttt cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg
     2401 agtttccacg ccaataagca cctgccactg ataataattt aaatacttgt atacggccag
     2461 tggatggaac atgtggtcca cgacacaaat cgatgaattc gccttgttga taagcagtaa
     2521 tcacttcttc ttctggcaat tcactaatca gttctacttt gtacggatca cttgcaaaaa
     2581 tttctaatgc ttcctgtttt gaaagaacac ggcgttcgat cggcagattt tctttgacga
     2641 ttttcatcat ttctgcttcg attgccggca aatcttctgc agttacttgg ttttgtccat
     2701 tatctgtatc gtagtaaaac cctgaatcga tagctggtcc aacgccaaag tggatatttg
     2761 gatagaggcg acgcatggct tgggccatca aatgagctgc tgaatgacgg actaagccaa
     2821 gtgcatcttc atggtctggt gtgatgatct caatacttgc atcttcctcg attggacgag
     2881 tcaaatcaac gagttttccg ttcactttac cagctaaagc tttttttgct aagctttttg
     2941 aaatgctttc agcaattgcc aatgttgttg ttccagactc aaattctttg actgcaccat
     3001 ctggaaaagt aatttttatc ataatagttt cctccttgaa atgttttgat tgatcagatc
     3061 atcaagctga aaaacaaaaa aatcctagca tctcttgaaa agagatacta ggacgaaaag
     3121 ttcgtggttc cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt aacggtccga
     3181 ccgcccttgc ttcaacaagg gtttcgaaag tggtcatcct ttggttttct cctggaaaag
     3241 tttcagccta gcttttcctc tcttatcgaa gactctcaaa ttcagttggc tttctcatca
     3301 atcaattatg tgtttattta accactaata aatagaaaaa tcaagtggga aactaaactt
     3361 attttttcca ttccccattt acaccgtatg ttcctttttt catctcattg atgatgacat
     3421 ggatgtgttc ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata tctttcatca
     3481 tattttctaa ttgttccgga ctgcgacctt ctactaattc gacatgtacg aatggcataa
     3541 tgctctctcc tctttctgat cgttcttttt tcaactcgat tctatcatac tatgtttgtc
     3601 ttgggtactg aatcaggaaa aatggctgga tctttttttg ctctcaaggc actcaaaata
     3661 gagaccgtat ccacgacttc ttgcaaaaca gctccgaata atgcggggat tagtcccgta
     3721 cttgcaatta acatcaaaaa aatacaaata aagatgccaa tcagtacaga ctgtttcgct
     3781 attctcatcg tatctcttga aatcactact gcttttgcta cacgcaagag atcatctttc
     3841 aagatgaccg catctgcact ttcactagca gctgtagaac catgagcccc cattgcaata
     3901 ccgatatctg cagcagcaag tgctggtgcg tcattcacgc cgtctcctac catcgcaaca
     3961 ggtctttgat ctttagggat actttctaaa atggtcagtt tatcttgagg caagctacgg
     4021 gcatgtacag aatctaattg gactttttct gcaatcgttt gagcaatggc agatccatcc
     4081 ccggtgatca tcatcaaatt tttcacatca agttttctta gctccttgat tgtctttgct
     4141 gcttctgggc gaatctgatc cgaaaatagg atcgttcctg caaaagatcg atcaattgtt
     4201 acatagattg ctgtattgga cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca
     4261 atctttactt catgattatt gactactgct tggattcctt gaccggtcac ttctttcagt
     4321 tgactaacag gatacagttg gctgtttttc acgttgttca ctaacgaacg agctaagata
     4381 tggctagatt cctgttctgc actagctgca tacagaagta gctcttcttt ttttattcca
     4441 ttgactggct gaatcgcctc tacttccaat atccctttgg taagcgttcc tgttttatca
     4501 aaagcaatcg tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc
     4561 ccgtttttac ttgaacggct cattcctgca actaaagcaa tgggcgcagc tagaatcagt
     4621 ggacaaggtg atgccactac cagtacctca gcaaatcgta aagggtcttt tgagaaccac
     4681 caagaaatac caccgatcaa ataagcaatc aacgtgaaag ggactgcata acgatcagct
     4741 aaacggacaa atcgggcagg cttttctttt gattcttcga ctagtttaat aatcatttga
     4801 tactggctgt ctgcggcacg tttttcagcg atcatttgga tcgatccgtc tccatttaaa
     4861 gaccctgaca taatctgatc tccaggtttc ttggtttccg ggcgggactc acctgtcaaa
     4921 gaagattcat cgaccgaaga ttctcctttg gtcactcgac catctgcggg tacaagttcg
     4981 ccaggcttga ctagtaactg gtcatcaata tttatttcat cgattactat atcctgcaat
     5041 tttccttctt tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga ctgcagttct
     5101 ttacccgctc gtttattcgc gtaatcctct agactatctc cgcccgtaag catgatcaat
     5161 atcatcaaac tagcccagta ttctttgacg gctaatgttg caaggattgc ggtaatcgct
     5221 aatacatcta cgccatatct tcctgaacgt aacgtcttga tcatttccaa aaacatcaag
     5281 aaggcgagaa tccctccagt aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa
     5341 acaaattcac ttaataaagc tatcccacca ataagcaaag taagtaaaaa ttttttgaaa
     5401 tgtgtcattg ttttttctcc tcttttatgt gacttagaga ggttagtaat aaatttccag
     5461 ctgcctcttt catcttaagc atagtccttg actagcactt tttaaaacaa taggccttaa
     5521 ttgaaaacaa ctatcactat cagcgtttcc aaaattcatc agttctacaa acaaaaatat
     5581 tcagcaagga ttactttttc gattcatcag ttaaaaagta ccttttttcg aaaaactatg
     5641 ctatactttt aattcgttat ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa
     5701 ctagaacaaa cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca aaatatgctg
     5761 gatacaaaac aacaagaatt gactcaggaa atgaatcatt cgacaaaaga cacagctaat
     5821 cacaagattc gagctggttc caatgaagct ttttatgaat ctgctgttga atatcgccaa
     5881 catgagcagg aattgctttt gaaataccat actcttgaag cacagcaaaa acgcctgcaa
     5941 acgttgtctg tcatgaaaga tagtccttat tttgcccgta tcgactttaa agaagaccgt
     6001 gaaacagaaa cattatatct aggtatcgct tctttaagag atctggcaga agaaacaatc
     6061 gttattgact ggcgcgcacc aattgccaat ctttattatg aaggtgagat tggtcccgca
     6121 ttttatgaaa ctgaaacaga tagaatagat gtcgatctgc agttgaaacg acaatttaaa
     6181 atccaagaag gacgtatttt atctatggta gatacgtcgg aagtaatcaa tgatgacttt
     6241 ttgctagaga tattggatga atcatcaagc aaccatatga aaaacatcgt ctctacgata
     6301 caaaaagcac aaaatgcaat cattcgggac acaacaagta aagtaatgct gattgaagga
     6361 atcgccggaa gtggaaagac atccgctttg cttcaacgta ttgcttttct gttgtatcat
     6421 aatcgcaagt ggctcgaggc tgaaaacgtt cttctatttt cacccaacca tttgttttct
     6481 gattacatct cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac acagacattc
     6541 aaaaattatt tggtgcagct gctgccagag ttttctttac tagatgaaca gcaacaagaa
     6601 agtggttttt tgctagggga aaaagatccg atacaagtga tgaaaagcgg attgacctta
     6661 gtcgatcaga ttgatcgcta tatccaaggc atcacaaact acggtccctt gttccgtgat
     6721 atgaaaataa atggccgtac gatcctatca aaggaatcga ttcgccaatg gtataaagaa
     6781 acaaatgaac aattaccttt acatcataga ctttctctat tacaaacgaa gctcctgaaa
     6841 aaattaggcg gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa
     6901 gaacagctcc aagaactata cgcaacagat ccaaatcttg aatatacaga acaaaaggag
     6961 cgttcattac gtaaaaaact ggccaaccaa atcgtacgta aaagattccg tcgaatccat
     7021 cgagggatca tgaattatca atttgtcaat ataacgaaac aatatctcca ctttctacaa
     7081 actgtgccac tttctcttct aacacgacat aaaatcagcg agaccaggtg gcaggaacat
     7141 ttgacaactg ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga tgctgtacta
     7201 ttctttttac tgatgcgccg aacatatcca gtgacggtta cacacaaagc tcgctatatc
     7261 tttattgatg aaatgcagga tttcgcacca tcacaagtgg cattgctgca agccttgttc
     7321 ccaaaagcaa atttgacttt ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg
     7381 atcgttggat cattggaaga ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg
     7441 acaagctatc gctccacaaa agaaatcact gattttgcta atcattttat tgtgaaagaa
     7501 catcaagtag aaacaacagc aagaaaagga gaccttcctt tgctcatcca aggaacaaca
     7561 gatgatatca aattgaattg gttagcagat accattcgaa cgactcaggg aaaagcccgg
     7621 tattggcgta cagcaatcat agctaaaaca caagaagaat gcgaagctct ttatgctcaa
     7681 ctgccagacg agttgaaaca acaagtccag ctgattttaa atgagagtga ctttatgaag
     7741 cgttccatta ttttacttcc agccttttta gcaaaaggtc tggagtttga ccgagttttc
     7801 ttgtgggatg tagatgcaga acatttttct tctcagcaag acaaattgat cctttacacc
     7861 atgtgtacac gtgcgatgca tgaattgacc ctttttacct ccaataatga aagtcctttg
     7921 ttaaacaata tggatatttc atcttatgag aaaaaagtac tgtaagtaaa aagacaactg
     7981 ctatcattaa tgttagcagt tgtcttttta tttacaataa taattgcaga aaaagtaatt
     8041 caatcattta aggtcattta ttctatatat aaatccaaat ctgtttcagg atattttaat
     8101 ttgacaagcg aaataatcat tttcagttct tccatgtttt ctggatattc tgggtcaaat
     8161 accttgctat gacggccaat cccgccattg tcgatcaatc ggtaaattcg attccattca
     8221 atgaattggt caattgattg gaaattgctg attggatagc cgccacaatc caaaatctct
     8281 gatacacgaa atgctaggta tgcactatcg atcactttat tttttttcgt gtctatcttc
     8341 tcatttcttt cgataagaat tttttcagga cgatgtttca agctccactc taatagtaga
     8401 aagaaagcct ccatccgata ctcttcttca aataaccgaa aggctgaatc agaacgacga
     8461 aaaagcagcg gaatcagata attttgtcta taaatcagca ttggtgcgcg gtccaccata
     8521 cactctaaat aacggttcag ataccctgta ccgacatagt ttcctaatcg atagccttca
     8581 acgccgaatc ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt cacagctaac
     8641 tcttctactt tgcggtaacc gcttaagata cgttcaaaag gttctaaagt gatttttgct
     8701 tccatattct tccccgtcca tcttccacat acttcatatc atttcacatg atacttatgc
     8761 tctcatcata ccacaggttg aattagaaaa gaaaataaat cacacaaaaa agcaagacaa
     8821 tctgttagtg agattgtctt gcttttcgaa aaagactgaa tacttttcgt taaaactggt
     8881 tgcttagtct tctaatgcca cattatggaa cacatgctgt acatcttcta gatcttccag
     8941 tgcatcaatc attttttcaa atgtttcttg atcttctgga gatagtgtta cctcgttttg
     9001 agggatcatt tggatctcag cgattgaaaa atcagtgatt cctttttctt ttaatgcttc
     9061 ttgtatacta tggaaatccg ctacttcacc gtaaacgatg atttgtcctt cttcttccat
     9121 tacatcgcga acatcgatgt ctttttccat taggtattca aggatctcgt cagcgtcttc
     9181 tcctgcaaaa gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc
     9241 catgttcccg ccatttttcc caaatgctgc ccgtacgtct gctgctgttc gattcacatt
     9301 gttcgtcaat gtatcaacaa tcaccattga gccgcttggt ccaaatcctt catagcgcaa
     9361 ttcagagtat gtttcatctc ctgcaccttt tgctttttcg attgctcggt caataatatg
     9421 ttttggtaca ttataagttt tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg
     9481 cggatcagga tcacctgatt ttgctgccgc gtagatttca atcccaaatt ttgcataaat
     9541 ccggctatta ttagcatctt tagcagtttt ctttgctacg atatttgccc atttacgtcc
     9601 catattttca cttcgctttc tgatttgata gtttcataac gcaaaaacgt cctgttccat
     9661 tatacttgtg aatcacgatt ctgtgaagct ttttttcttt cttccttcat tcttctcatt
     9721 tttagtcaat tatctatact cggattggga tttgcccgaa attctgttcc ggaatattgc
     9781 ctcccaattg atagatttga tcacttctaa ctaacagttc cgcttcttgt gcctcttttt
     9841 ttccgattcg ggaataaata gaaatctctt ttttttcttt taagaatctt cttccagcat
     9901 cggtaaatcc aagtagtcga agataatttt tattttgttg gacaggaatc tcttgctttt
     9961 tgatattcag caagacataa caagataatc gttggatccg tgtccatgta tatctttttg
    10021 tttttacctt tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct gttttcatgc
    10081 gcgattctat tccttcggtc atctgataga tatctgtcag ttccagcaaa gtagaagata
    10141 acaaccgata acgcaaatac ggccaataag attcccaagt ctgcagcgaa caagttgaaa
    10201 gatcttcttc tgtctgaggc ggcacaagtc cagctattct ttttccttct tctaacgcat
    10261 ggcgaatggc tgttgcactg gctatcctct gatctgtgat gattttttca tgatagcctg
    10321 cacttttacg tcggatcgga tgtaattcca tagggtggac atacttggca ttttcctttg
    10381 cataacttaa tcccaagata tgatttggcg aagaaaaatc aagcgtaata tcgggataca
    10441 gactgcgaaa aacctcagtc attttttgcg gataactata cgtttgatca gacagttctt
    10501 gaaatgcctc ttctatccta tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat
    10561 caaacgaagt ctcttcttct gttccaaaac acaaactgtc acagtccagt gtttgcaaga
    10621 tttttactgc acctttagca aaatagtcag ctgactgaac cgcccattcg acgggaagct
    10681 ccactactaa atccacacca ttttgtaaag cttcattcgc gcgatgccat ttatcgatga
    10741 tagccggttc gcctctttgc aaaaaattcc cgctcattac agcaatgacg acctctgctc
    10801 ctgttatttt tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa ggattgtact
    10861 ctacgataat cccgcagctt ttcatgcgca tcctccttca tagttatcga taaaaaagaa
    10921 atagagacaa gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata taaactattc
    10981 tcgcaaacac aatacttctg cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt
    11041 cggatatata atttatgttg aagatttatt cttttcttgt taactcgagc aggcaaaacc
    11101 gctagtcttt gtatgctaca aagaaccagc gctttgattt ctctgatgct gcttcgtctg
    11161 taaaatcaga aaatgcttcg actttcgaaa agccgctgtt ttccagcatt cttaaataac
    11221 tttccaatgg ataggtacgc tcttgatgta gttcatcttc acgaataaat cgaccagacc
    11281 cttcttgttc ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg
    11341 gataactatc ccacaaaaaa gcaaaattat ccccttgata atgatagcta tattctggaa
    11401 aaacctcatc tacttgatag gtggagtgta cgtcaaataa aaatcggcca ttttcttcta
    11461 acgcttgata gacttcatca aacacttgct gtacctcttg tctatttttc atatagcaca
    11521 gcgaatcaga aaaacaggta attgcctgat attgtccgat ttctgataaa tcgagcatat
    11581 tgccttccac aaattggatc gatgcgtctt cttcatatgc tcgctggctg gcaatcatca
    11641 acatctcttc tgaaaggtct aaccctgtca ctttatatcc atcttttgca aaggcaaccg
    11701 ctaatgctcc agtaccgcat gccagttcca aaatattttt tgtgtcttct tgtaaatgac
    11761 gtttcgaaaa agctaaccat tgctgataaa gtgtttcgtc catcacttca tcgtaaatga
    11821 aagcaaatgt ttcataagcc atatgttaat ccaaccattc actaagatcg actagcggag
    11881 catctgacca tagtttttcc agattataga aatttcgttc tggcgcatgg aaaacatgta
    11941 cgatcacatc tccaagatcg atcaataccc attttccgcc ttcttttcct tcaatacgtt
    12001 ttacttcata tttgttttct tcttctttat caatgatttc ttctgtaatt gcgttgattt
    12061 gacgttcgct atttgctgaa cagatcatga aataatctgc caacaaggaa acatttcgaa
    12121 cgtctaaagc gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta
    12181 attgttcttt actatctatg atgatttcct cctaatatga gcagcttatt tttttgctac
    12241 ccagtaatta tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg
    12301 ggccaatgta tgttttgttt caaatgctac agcttcgtct agatcagccc atgcaatggc
    12361 tcgtgcatct tgtaccccag gaaaatctcg acctggttca atatagtctg ctacataaat
    12421 gattttgtca aggagactca tcttagcagc cccagtcgta tgcacccgga ttgcatgaag
    12481 aatctctgca tcggtgattc ctaattcccg ctctacaaac gaagcgccaa caagaccatg
    12541 ccagattgca ttattataat tcaataactc cggatcaaaa ccgtctctta cgattaccat
    12601 tttgaactcc tcatctggtc gttcttttgc ataatcatgt gtcaatgcag caatactagc
    12661 tttttcagga gaagcaccgt attttttggc aagagccacg gctgtttctt ctactccaag
    12721 cacatgcttg aagcgttgtt cgctcattct catctgtatt ttttgcatca ggttttctct
    12781 agaataagca gtatattcat tactatagtc catattcata caacccttca ttttgaatat
    12841 aatcaattac tttatctgga acaaggtagc ggatagaaca gccttctttg atcttttgac
    12901 gtattttcgt ggaactgatg tcgatctcag gtacatctac ccaaattaca ggatatggag
    12961 tatccgttgt gtatcctggt cgacgtatac ccacaaagtt gaccatagaa gtcagctcat
    13021 cgattttata ccattttggc aaatattcta ccatatcccc accgataata aagtaataat
    13081 ctgtatccgg attgttttgc gtcaattcct tcatagtatc ataggtgtaa cttttcccac
    13141 cacgagctaa ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc gctaattcca
    13201 acatgttgag tcgatgtttt gcatcaatcg tctgtttctc atcaacatga ggtggctgat
    13261 aagaaggcat caaaaatacg cggtcaagac ctaaattccg gccagcttgt tcagccatca
    13321 ctaaatgtgc aaggtgaacg ggattgaatg ttccgcccaa gatccctact tgctttttct
    13381 tctctaagaa ttgaggcatt tcttcaggaa ataattgtga acgaacaaat gtcttagctt
    13441 gcgtattcat catggtcact ccttatattg cttttacttc ttttgatagc ctttgatact
    13501 tttcttttgt ggaaggtttg tataatacaa taacacgtcc aatgatttgg acgatttcgc
    13561 aatctatttc tttttctaat acttgagctg cttcttctgc aatctcatct gtattttgaa
    13621 gtaacgatac tttgatcagt tctctttttt ctaaagcttc ttcaatttga acgatcattg
    13681 ctgaattgat cccacctttt ccgatttgaa aaatcggctg aagatgatgc gctttgctac
    13741 gtaaaaaacg cttttgtttt cctcttaaac tcataatttt ccttcttcct taaatcaatg
    13801 ctttgcgttt caatacatct acaccctttg gcgcccagcc cgcaattact actggcgctg
    13861 ttacagtgat ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc acagaaaact
    13921 caaaacgaac aagctccgga aatgcctcga cttcatcagg acgaggaggc tgtaataatc
    13981 cacctacatg tttttgataa aattcgtctg ctttttccag tttcgttcga tgaatattta
    14041 catcatttga cacataagca ataaatgaag cacgattgcc gctgataaaa tcaaatcttg
    14101 caagtccgcc caagaaaagt gtttgccctt cattcagctg ataaacttta ggtttgattt
    14161 cttttaacgg tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc
    14221 gatggataat tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga atctcgattt
    14281 tatccaaggt cgttcctgga aactgcgaag ttgtgattac atcttgtact cccgctgtat
    14341 ttttgatgat ttgattgata agcgtcgatt ttccgacatt tgttactccg acgatataga
    14401 catcccgtcc atcgcggtat tcttcgattt tttctaatag ttccgtcatc tcatgagatt
    14461 tcttagcact agttaataat acatctactg gacgaattcc tgcttcgtaa gcacgttcct
    14521 tcatccattg tgttaattta gtttttttca atgatttagg taatatatcc actttgttcc
    14581 caactaaaag tactggattg tcaccgacaa aacggtgtag tcctgggatt agtgaaccat
    14641 tgaagtcaaa aatatccacc acattgacga tcaatgcatc tttttgccct aattcattca
    14701 gtaagcgcaa aaagtcgtca tccgtcaatg agacatcttg ggtctcatta taatggcgta
    14761 atctgaaaca acgctgacaa tagacttccc cggaagctaa acctttttcc aaagccgttt
    14821 gcggcgtgta tcctaattca ccgggtttgt ttgtttgtat aacggcacca cagccgatac
    14881 attgtaattc ttctgtcatt cgattccacc tcgccagccc atatcagggt gttttgctaa
    14941 tagataggcc ataattcttt tttcccaaaa acgattaaat tgtgtcttcc aactatctgt
    15001 tgcgataatg ggttttacaa ggatgctacg tatccctgca cgattggctc cacgtatatc
    15061 agtcataatc tgatcgccta ccattactat ctcatcatct gctaaatcca attgtttctt
    15121 tgctatgttg atccctcttg ctaaaggttt cattgctcgt gcaacataca aaagatcaaa
    15181 tttttcaatc gctcgcgcga cacgttttga attattgttt gaaacaacaa caacagggat
    15241 tcctgcattt ttcatttcta aaatccaagt aagcaactct tctgttccat ctggattatt
    15301 ccatgcaatc aacgtattgt ctaaatcggt caatactgct ttgatgccta gattctttaa
    15361 ttgggcaggt gtaatctgat agatggcttc taccatccat gttggtttgt aatttgaaaa
    15421 catgctgtct cctcatctgc aaaaaatgaa ggagcacata aagtactcct caaccccgta
    15481 ttatactgca tatcagcaaa aaaaactacc tctatactcg ttcgtctttc agaaaaagca
    15541 tttctttaca aaatgaccaa aaataaagat tgatcacaga aaaatttaga aacaaaaaac
    15601 aactaatatt ggcggaacct cttgtatcgt ttttccagta atttatgctg atctaatcga
    15661 acaagttccg tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt
    15721 ggcaactgct cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt
    15781 agttctggcg cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc
    15841 cacaaaatcg acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc
    15901 cagacttcat ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg
    15961 atggcgatga tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct
    16021 ccttcgccgc gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaaggta
    16081 atcaccggtc tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct
    16141 tcaggatggg gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga
    16201 ataccgatca ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg
    16261 tcatctgcaa ataaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa
    16321 aaatccaagc ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc
    16381 ttatccacga tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg
    16441 tttcaattcc atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc
    16501 cttttggaaa tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc
    16561 aaaaccgatc aagctttggg gttctgctag aataatatcg ccttccattg caaaactggc
    16621 agttactcct ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct
    16681 atgccgtttg acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg
    16741 catccgtgcg ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg
    16801 ttcgaataat ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt
    16861 aggatccatc actccaataa tgaagggaat cccctgaatg gttgctttac cagtgaggac
    16921 tgcttcatga agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa
    16981 tgctagcgga tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat
    17041 aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac
    17101 tttttcttca ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca
    17161 catgttatca ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg
    17221 aatatatttc tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt
    17281 ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc
    17341 aggatgacta atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa
    17401 ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat
    17461 tatttttgca atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact
    17521 gtctactcgc agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga
    17581 aggagcaaaa tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg
    17641 gaatactact tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt
    17701 ttttactaaa tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt
    17761 attcatttcc atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc
    17821 attttcataa ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt
    17881 tgtttgtgag atcactacag aaggagattc ttccagtact ttttgattgt tgcgttgtaa
    17941 agaacaatct cgctcgccta aatgaatgac atgaccaaat cgatcaccta agatctgaac
    18001 ttctatatgg cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc
    18061 cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac
    18121 tttccgaatg cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat
    18181 tctgtctgct atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat
    18241 gactgggaca tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc
    18301 gatcgttgca cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa
    18361 tcggctattt tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat
    18421 tgcagcgctc agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat
    18481 gcagattgct tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat
    18541 cgctacggtt tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc
    18601 gcggttagct atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt
    18661 ccgcttcggc tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag
    18721 ctttcacttt caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac
    18781 ggaattttgc tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag
    18841 aaagtaatgc aactgctcct gcttgagcca ttgcttcaac aatcaatacc cctggcataa
    18901 caggttcttc tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag
    18961 caacgatccg ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac
    19021 gatgtggaat gatttctttt atttcttgta tgttcattgt tttccctctt tcctgctttg
    19081 cttatttttg ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta
    19141 caaggatttc tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg
    19201 cttcaataat gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg
    19261 gtttgtcggg cgctggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc
    19321 catcaaatac tgccgattct ttttctttca ttatttctgg agcagttgtt gcttgttcag
    19381 gtatgacatt gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc
    19441 ctcgacttgt tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt
    19501 ccgttagcga ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa
    19561 ttattcctcc cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt
    19621 actcattgca taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat
    19681 ctcaggatct tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag
    19741 caaagttgcc aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt
    19801 cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc
    19861 ttctgcctgg tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc
    19921 cggctgtaca ccagcttctt ccatcgcaag cttcattgct ttagatgcac cagaaccatc
    19981 tggagtagga gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc
    20041 atagattttg gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc
    20101 tccttcaccc atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg
    20161 atcagttgat tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat
    20221 cgttgcttct gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa
    20281 tgcttcccca attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc
    20341 ttttgcacct acacgcaaag caatatttcc agctgccatg ttggcaatcg ccattggtac
    20401 gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat
    20461 tgttggaaga ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt
    20521 ttctttatct aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga
    20581 aaacatatcc attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt
    20641 gacttcaccc gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat
    20701 gccattcgtc ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga
    20761 agtgactcca tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc
    20821 gtgcatgacc aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc
    20881 taagaaaact gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg
    20941 cttttccgct tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa
    21001 tcctggcgca atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt
    21061 tgttaatcct actacgccag ctttactagc tgcataattt gcttgaccaa tatttccaat
    21121 caacccagat acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt
    21181 caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg atatccagac attttttgaa
    21241 atcttctgca tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa
    21301 cacatggatc gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata
    21361 atcagagatg tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat
    21421 cttttctttt ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa
    21481 agctaaggcc atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc
    21541 ttttacatcc ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta
    21601 ttctcaacac gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact
    21661 tttccggggc cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc
    21721 tcatagaaac gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc
    21781 ttcattattt ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc
    21841 tgacttaggt ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc
    21901 ccgctgacat ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct
    21961 tcgtctactg ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca
    22021 ggagatacga tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc
    22081 gcattcatca ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg
    22141 cctctttttg ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat
    22201 gccgaatatt ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc
    22261 aataagcgat aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga
    22321 tccaaacgtt cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt
    22381 gcttcatcaa atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga
    22441 tattgtgcac cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc
    22501 agaatatatg tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg
    22561 tcccagcccc caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa
    22621 atcttgaacc gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat
    22681 ttcattgatg atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt
    22741 agtcaatttg atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt
    22801 ttcgaatacc ataataatat attcctccga tattgtttgc tttttatttt tataatttaa
    22861 cagtctacaa ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata
    22921 ttactttctg ctgttttcct aaggtgatct ttccactaga aactgcttca tccaataaga
    22981 tagggatgct tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt
    23041 gcggcatctt tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa
    23101 gcagataatc aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta
    23161 cggcaaaatc aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt
    23221 ctgaatagaa cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac
    23281 cgtcagctgc taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc
    23341 cacctgctcc atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag
    23401 atagtgtttc accaccaatc accagtccct tagttgagcc gcagcggatc attttttcag
    23461 ccatgcttaa cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat
    23521 gagttgctcc caatttccct tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg
    23581 acattgttgc tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca
    23641 atttttccgc cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt
    23701 cacgaatacc cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat
    23761 cattattagt aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt
    23821 gttccattaa atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat
    23881 ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac
    23941 gatgaaaatg ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta
    24001 caacgcggcg gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt
    24061 taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg
    24121 tttttttctt gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt
    24181 tgaattgaga ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa
    24241 caagataatc atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt
    24301 tgattatcaa atcttttgat gttcaaacta tatcttaact tttttcaaaa tgcaacctta
    24361 aaagatcatt tttttactat atgagctttt
//
LOCUS       DAZUMI010000032        23847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000032 DAZUMI010000000
VERSION     DAZUMI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.032"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..403)
                     /locus_tag="ACG81C_001116"
     CDS             complement(41..403)
                     /locus_tag="ACG81C_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS5064293.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(592..774)
                     /locus_tag="ACG81C_001117"
     CDS             complement(592..774)
                     /locus_tag="ACG81C_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064294.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            749..2188
                     /locus_tag="ACG81C_001118"
     CDS             749..2188
                     /locus_tag="ACG81C_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5064295.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(2195..4747)
                     /locus_tag="ACG81C_001119"
     CDS             complement(2195..4747)
                     /locus_tag="ACG81C_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS5064296.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(4861..6192)
                     /locus_tag="ACG81C_001120"
     CDS             complement(4861..6192)
                     /locus_tag="ACG81C_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5064297.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(6158..7636)
                     /locus_tag="ACG81C_001121"
     CDS             complement(6158..7636)
                     /locus_tag="ACG81C_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5064298.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7941..9776)
                     /gene="tdc"
                     /locus_tag="ACG81C_001122"
     CDS             complement(7941..9776)
                     /gene="tdc"
                     /locus_tag="ACG81C_001122"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5064299.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            10246..11673
                     /locus_tag="ACG81C_001123"
     CDS             10246..11673
                     /locus_tag="ACG81C_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5064300.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(11765..12130)
                     /locus_tag="ACG81C_001124"
     CDS             complement(11765..12130)
                     /locus_tag="ACG81C_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5064301.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(12127..12492)
                     /locus_tag="ACG81C_001125"
     CDS             complement(12127..12492)
                     /locus_tag="ACG81C_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5064302.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(12684..12932)
                     /locus_tag="ACG81C_001126"
     CDS             complement(12684..12932)
                     /locus_tag="ACG81C_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064303.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            12931..13059
                     /locus_tag="ACG81C_001127"
     CDS             12931..13059
                     /locus_tag="ACG81C_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5064304.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            13116..14810
                     /locus_tag="ACG81C_001128"
     CDS             13116..14810
                     /locus_tag="ACG81C_001128"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5064305.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            14910..15206
                     /locus_tag="ACG81C_001129"
     CDS             14910..15206
                     /locus_tag="ACG81C_001129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064306.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            15584..16717
                     /locus_tag="ACG81C_001130"
     CDS             15584..16717
                     /locus_tag="ACG81C_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5064307.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            16748..17068
                     /locus_tag="ACG81C_001131"
     CDS             16748..17068
                     /locus_tag="ACG81C_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5064308.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(17097..17561)
                     /locus_tag="ACG81C_001132"
     CDS             complement(17097..17561)
                     /locus_tag="ACG81C_001132"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064309.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(17692..18945)
                     /locus_tag="ACG81C_001133"
     CDS             complement(17692..18945)
                     /locus_tag="ACG81C_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5064310.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(18974..19291)
                     /locus_tag="ACG81C_001134"
     CDS             complement(18974..19291)
                     /locus_tag="ACG81C_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5064311.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(19317..19637)
                     /locus_tag="ACG81C_001135"
     CDS             complement(19317..19637)
                     /locus_tag="ACG81C_001135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5064312.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(20101..21516)
                     /locus_tag="ACG81C_001136"
     CDS             complement(20101..21516)
                     /locus_tag="ACG81C_001136"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5064313.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(21533..22849)
                     /locus_tag="ACG81C_001137"
     CDS             complement(21533..22849)
                     /locus_tag="ACG81C_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5064314.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(22999..23697)
                     /locus_tag="ACG81C_001138"
     CDS             complement(22999..23697)
                     /locus_tag="ACG81C_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5064315.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         7457 a         4927 c         4012 g         7451 t
ORIGIN      
        1 aacatttctg tcacaacctc ttctctattg tcctagacat tcattgcgtt gatttaaaac
       61 ttccatcaat caaatctgct agttcatcta gcttttctat tttacgtaga tcaatagaga
      121 tccaatgttc tttattcatg tgataggctg gaaaataccc ctcttttcct cgcaaaattc
      181 cgccaagttc aggatcgacc ttcaaatcaa gaatattgat gatcctatgg tcacttgcat
      241 catcatagat tttttcttct ggaacagaca tcaggacccc aaaccatttt ttgctttttt
      301 gatgtctgaa tacgcaataa tcaggaaatt ttttaaaaat atattctgga gacaaattgt
      361 atgtttcatt gatgtgatcg attagttctt ttttcgttag catctgcttc catctctttc
      421 ttattgattc gctttattca aagataccac gtaagaatag aaaaatgaat acaagtagtt
      481 gctaatcaaa tacttcgata tttttctttt ttatcaactc ttaaatgttc aatccgcctg
      541 tgttggtctt gctaccatct actattaaaa tgtttattat tgtttgccca cctatttttt
      601 gattttagtg gtatcatttt tcaagttcat gatgaaattc ttatcatcag taaatgctcc
      661 agaaaaataa acaatgattg aaaaatcagt tgaaacttga ccaccgttta ttttctccgt
      721 attcttagaa tagaagggag tctgattgat gtattcacta atgaaaaaaa tcattaccga
      781 aaaagatatt cgacgtcatg tttctttagt agaacaattg ctgaatcata caaagataac
      841 cgtaaatgaa ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca
      901 atctattcgc tctcagctgc ccgaaggctg ggacatcatc agcgatcaag caggaatcag
      961 tttgcaaaac cagcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc
     1021 agtcagtgtc gaactgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt
     1081 tctagccgac catgggcttt cttatgggac attgaaaaga catgtgacta aaatcaatca
     1141 gcgtttagca agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg
     1201 aaaagagcgt gcgattcgta ctttttatca tcgattgctg attcctttca ctcataacaa
     1261 ctattttttc gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct
     1321 cagtcaaact gaactagcgg ttgaaacaga agagattttc ggaacatgtt ggttctttat
     1381 caatacgatc cgtataaaag cgaattgccg attagattct tctatccata ccaacagcac
     1441 actctcctcc ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct
     1501 taaagacact gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa
     1561 taactatgga caagaaaccg ctcgctgcct gcatcattct ccatttttag aggtcctcga
     1621 aacgtttgta gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct
     1681 gactgataac ttggctctcc tcatattaaa atatcatgaa tcgccgatat tgattgaagc
     1741 attggaccgt caataccatc gttttttaga aacatatgat cagcaatatg cacacttgta
     1801 tcaacagaaa gagaatttac tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa
     1861 ttactttctc caactcttgt ctctactgat ccaacaaacg attttttcta tcaaaccgaa
     1921 tttgttcaat gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca
     1981 ggaattaaaa gattacttag gaaaacgtgt ctgcttctta cctatcgaac taaccgatct
     2041 ttccactatt tcttttgaaa aaacagatat cttgatttcc aatttccctc tagattctct
     2101 agatatccct attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac
     2161 tgaactcact ttcaaaactt ttttatagta acggctagcg actgtattct ctagctgttt
     2221 tttctttttt gcgaaacagt ttgttcatga tccctttttc tatttctaca actaagaata
     2281 gaagaatcac atttgctcca atcaataact gttgtgtcat cgttaaactt actgtaccga
     2341 tcagctgctg gccgactggt agaaaaataa ccatcccttg caataaagca aggataccta
     2401 atgaaagaaa aagcgctttg ttttgcaaaa gaccttggtt caatgacggc ttggacaact
     2461 ctctgcaatt gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt
     2521 gctgtaagct ttgcccttca aactgcatag ccaataaata ggcaggaacc atgatcagta
     2581 gggagacata gatgatccga aacaaatgat aaccagatag gattccttgc tttggatctc
     2641 ttggtgggcg cttcatgatt tctggatctg gcgactcgaa ccctagtgca taagataaag
     2701 tgattgtcgt taccatattg acccataaaa tctgtactgg ggacaaaggt agcgggcgat
     2761 tcagcataag tgcccaaatc aagatcaatc cttgagctaa tgcagttggc aagaagaaat
     2821 tgatggtttt ttgcaaatta tccaaaatcc gccggccctc ttttacggct tttgcaatcg
     2881 tatggaaatt atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt
     2941 tgattcccat agcaattcct acatcggatc ttttcaaagc aggtgcatca tttacgccgt
     3001 ctccggtcat tccgacgatt tctccatttt tctgcaaagc tttaacaatt cgcagtttat
     3061 gttctggtgt cgttcgagca aagacatcga ctattggcac ttgctgaatc aactcttcat
     3121 cagacataag atccaaatcg atcccttcta aaactttggc agtatgtttt aagccaattt
     3181 gttccgcaat cgcttttgct gtgtctttgt gatcccctgt aatcattttc actgagatcc
     3241 ctgcttgttg gcattccttt acagctttga ctgcactttc ttttggcgga tcgatcaatc
     3301 cggcgatccc tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat
     3361 ctactttttt ataagcaaat cctaaaacac gctgaccttt tctcgcaaat tctgcagctg
     3421 tctgactcca tgcttcttgt tctgcagaag aaagagttga tttagcaaat aatacttctg
     3481 gtgcaccttt gacgtaaatc accgctcctt tttcctccgc atgcatcgtt gccatatatt
     3541 tgtattcaga actgaacgga atctttgctt ctattttttt caaaggtaga ttggctgttt
     3601 ctgtgtgtcg aatcagcgca acttctgtcg gattgccact taaattttct gttttctgtt
     3661 cttcttttgt ttttaattcc tgacagtttt tcataatgga caacacgcag gcttcttctt
     3721 ttgcagctac atccacaact gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac
     3781 agatcactgt catagaacct aatgtttcta ccgaaggaag tcctttgatg atcgcctttt
     3841 cttttgccat ttcatgtact ccaacggata gaatcatggt caaaacagct ggcaatcctt
     3901 cgggtaccat tgacaccacc aaagcaatcg tagccgaaag aagaatatgc caatccatac
     3961 catatcgaaa tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg ccttgaaaaa
     4021 tttgtttgtt taatcggttg atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt
     4081 gcaatgcttg attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga
     4141 ctgcagaacc agattgtaca agcgttcctg aaaaacccat atttttctgg tcgccgatcg
     4201 attcttcttc tgtcaaaacg gcatgacttt tttcagatgc ttctgattct cccgtcaaaa
     4261 tcgattcttc cactaataaa tcatgtgctt cgattaatcg gatatctgct ggaacgacat
     4321 ctccggcacg taagacaatg acatctcctt ttaccagttc tttcgcaggg attttttctt
     4381 gcatcccgtt tgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg
     4441 attcttccgc ttttctttct tgccagtaac taacgagtcc attgacgatc actacgaata
     4501 aaataatact tccttcaata tattcactag aagcaaattt caaaaaagag gcaatgatca
     4561 acacgatcat cagcagatca gtaaaatgct ttgctatttt tttccatcgt tttgtctgct
     4621 gccttgcttt tatttcatta tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg
     4681 acaagccttc tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc
     4741 agttcatcct atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc
     4801 aggcgtatcg attatttcag gggggtctga aataattcgt tttgcaaaat gtatctgggc
     4861 ctaactgata tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat
     4921 aaccaataaa aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga
     4981 agtaaaaaac accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc
     5041 tttaataaat tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt
     5101 tgtcatgcct gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca
     5161 aagaccggca aaaaataaca ttcctgtaat taaaataccg tgtaaaggta cgcctttctt
     5221 agaaagcgca caaaaccaat ggggcaaact tttttctttt gctgtttgat ataaaatcct
     5281 gctagtactg tagactgcac tatttgctga ggacaaagaa gcaaccaaaa tcattagatt
     5341 gacaattgct gctccgcctt ggcaatggat cacttgcagc atagtcacta aaggactttc
     5401 ttttaaatct aaacaatgcc atggaacaac aaataaaatc gacaaaatcg ttcctatata
     5461 gaaaaagaga atacgccaag gcagacagtt gattgctttt ttcaaattct tttgcggatc
     5521 acttgtctcg ccaacggtta agccgatcat ttcaattcca gcaaaactga ataaaaccat
     5581 aggaaatgag actaccattc cagcaaagcc aaacggtagt aattcatcga tctgtccaaa
     5641 attttctttg attccgatag actgggtatg aaaatctgag acactcaatc ctatacccaa
     5701 taagataaaa aggaaaatcc ccaagatctt gatgattgag aaaaaagatt ccaattcacc
     5761 aaaccacttg actgcaacaa ggttgactgc cattaagatc attagtgcgc tacctccgct
     5821 tatcaaagct ggtacatgta caaaccagaa atggatatac aatcctattg ctgttaattc
     5881 cgcaagtcca gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa atgcccactt
     5941 tttgcctaaa taatagtcaa cgaaatcact gatagagtta aatgtactat ttgctaaaaa
     6001 taaatcaccc atggcccgca tcaaaccata aatagccaaa ccgcatatca aataaacgag
     6061 aataacagct ggccctgctg atgcaattgc ctttgctgaa cctaaaaata atcctgttcc
     6121 aattgcgcca cctaatgcga ttaactggat atttcgatta gttagagtcc gtttcattgc
     6181 cggtttttcc atcaagttca cctttttctt tttcaagcag catggcgact tcctgttccc
     6241 aagaaggttt ctttattttt tcaatacaga gtggtactac aaacaaaatg acactgatgg
     6301 ctgtgactgc aattagattg atccatgttc cagctttcat cgtcagtccg acagaaaatc
     6361 cgattgccgc aaataaaaca ataacgacag tccaaactcc aagattgctc tttttctctt
     6421 tttttccaat tctaaatggt cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg
     6481 cgatccccat gatgacatag acgatacagt agataacagt agttgaattt acgagcatca
     6541 aaaatgcttg attgacgcca ggaatcagca aataaaaaag tgcaaagatt gagataatga
     6601 cagactgagt caaaataacg gatttggata cgtcaaattg attcgtcttc caaaaattga
     6661 attttggtgg atacgcacct tttctagcac tttctgtcac tgtctttgct ggtcctgagg
     6721 cccaagcaga taattggact gctacaccaa tgaagacaag taaactaaac agattcacaa
     6781 taagatgtgg caaaccaaga atttggcaat aaatcgaaat agattgcgcg atattattca
     6841 attccatttg tccttttggt acgacatttg ctacaactaa agcattcaaa atattgacga
     6901 aaaatataaa aattaacgcg gcaaatactc cgcgaatata cgttttaact ggattttcca
     6961 atcgagtgat atacacagag gacatttcga taccagtaaa aataaacatg atcggcgcaa
     7021 aataaacaaa tgaggatgca gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc
     7081 ctaatgttcc ggttggtacg atcccaactt tgatccacgc agcaaaccct aataagagca
     7141 tcatcgtcaa aggcacgtac aatcctaacc aaacaccgac ttttccgccg atttttgcca
     7201 tatcaaattt caaattcaaa aaagtgatga tccaataaac gactaaaatg acaactaatg
     7261 taaatttgct gttcaaacca agcggcacat tatcgataat attaccaaac aacggagcga
     7321 gtgctgaagc gaccatcacc atcccaggaa acatttggac ccataacagc catgagacaa
     7381 cgaaccccca tttttttcct agagcattcg atacccaaag ttctggacca cctttttggg
     7441 gaaacattcc agcaaattca ccagagatca atgagattgg aaaagcgtac aataatactg
     7501 caaccagcat gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga atatttcgta
     7561 cacttgctac aagggcgcag gtcattccga taaaggtcat ggtcgtgatt tgctttgatg
     7621 aaccagtatc tttcatcttt attccttctt tcaatagcga catatgtttt tgttttcttt
     7681 tatttttgtt caagaacaga aatgacaagg tttcatcgat tcagtacaaa acccagtatc
     7741 accgaaacat ccattgaacg atcttagtga actgggtttg atacatgaaa cgaacgatcc
     7801 aacttataga acgttttata aaaaagaata tcttggcgag gaatatatga aaaacagggg
     7861 gaaatacaat tgttttgatg aagcaaataa ttgtctgttc tccatatgtc tcttgttatt
     7921 ctttcacaca aaaatcagtt ctattggatg atttcgttta gtttcttttc cattgctttg
     7981 gcgatttttt ttgtataaaa attgaaaatt cgatcatcat tcaaataagg agtcatgata
     8041 gccgcacgta gcagagtgat tttctgttct tttttccatt cttctttcgt tagacccatt
     8101 ctttcaacaa aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt agtatgagaa
     8161 gtaataaacc gttcaccata aacatcgcca tccatataag aagaccgatc gaacattttt
     8221 tcatttaatt cattgatagc gttcaaatct gtacagcctt gttctttgaa tacccaattg
     8281 accatattaa aatcaggatg attcaatgga tagacttcga ttgtttttcc tttaacagta
     8341 aaagtcagat gatcgagaaa ttctctgaat ctttgagctg cctcgattga agccccaatc
     8401 aattgaccat atccggtcac attcaatggc aatacacgat gtgctgtcca gactgcggcg
     8461 gcggttgctc cagcttttga tccttccaaa atataagctc ctaacatatc tggagccttt
     8521 actgattttt caaacacata tggcgcgaaa taagaaatga tatttctcat atttttatgt
     8581 ttgattacga ttcctccagc agcataaggg acatagccca tcttatgagg atctatcgtg
     8641 acagaatccg cttctgaaat agctctgaac ccttcatata cctctttgtc aatcgttacg
     8701 cagtggtgga aaacatgatg ttcttcaaag aattcagcta aggaagcata cggcacaaat
     8761 tctcctgctt cgtttagaaa cagtgaacgc gcatagccgc catatgcggc atcaacatgt
     8821 aaatagaaat agatcccttc atcttttaac ctttctctta attggacgat tttatctaca
     8881 ctgtctactt gaccttcttc tgtcgttcct acgacagcaa ccacacctag aattgggatt
     8941 ttttgtccag ctaattcacg aattgttttt tccaaagcat taatatccat tcgataatcc
     9001 tcttgaactg ggatagcaac catctgatct aagccgactc cgcaaatatc taacgccttc
     9061 atccaagaat agtgttttgt ttgagggacc agccatttcc ctaatctttg gatatttttt
     9121 ccgctacggg aagaagctgc tttgacctca tccatttctt cgtccgtaaa gctttcggtc
     9181 atttccaaaa tttcttcaac agataaattc aaaagttccc actcagacat ttttttcact
     9241 ttttcaggca gcacttcctt gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat
     9301 aatccttcaa gattagcaat agaaccatct gctgcaatgt gtccccatcc gtctgtcata
     9361 ttgaataatg aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt tgccatagaa
     9421 gattccaatg caacattatt tgggttccac agcatcgcat agttgtaagc taataaagca
     9481 ggcatcaatg tttcagcatt catttgtccc cagtaacggc ctgctgaatg ccatgggatc
     9541 gatcctgcgc gtaatcgctg actgacttca tccaagacct ctatcatatg gtcgcgggtg
     9601 gctagataat ctggagtgag cttatctcct tcgctaattg caggcatatc tgaaggaata
     9661 taattttctc tccatccaag atgttcatct accatcttgt tcaacaacat tttataaact
     9721 ggtccatttt cagctttgtc gcctataaag acggccttga tatccatatc tttcattttt
     9781 cttccctcca tattttgttg gtcaatgtct tgcagataca tgtgtttatc tgtaacacct
     9841 cttcactata gcgccaaaaa tgagaatttt ttttttataa catcaaatcc acgtagattt
     9901 ttacaaacaa aaataaataa agtcggattt ttctgataat tcaacgcaaa aaagcgaaca
     9961 atttttcaat aaatcagtct tctaaaatag ataacctaaa aaaagcgaac aaacaaataa
    10021 aatgactgtc cttcattcac taaacatatt tttatttttt tatattgtta aaaattcgca
    10081 atttattttt taatagaatt atctgttttc caaattttat aacataaaaa acctttttta
    10141 acggcattta tatgcttttt cagaaattaa ctgatgattt caaaaagaaa acatgtcata
    10201 atatttccca tattgtgcaa aaaaatacaa ggcggaggct ctactatgat tgaagaatat
    10261 attgagaaaa atattttaag acagctgttt ttatgtgggc aattctatgt aaataaagaa
    10321 gtcaatctag aaaagctctc caatctcctg catgtttgca aaaccacttt actcaatgat
    10381 atcaataaca taaaaaaaga atttgaagaa cagatcgctt atacacatcg ggaaaaggac
    10441 cgttacactt tatatttttc agaacatata cctcgatgca agatcatgca gcaattgtct
    10501 caaaattcgt tgtttttaaa aacttgtctg ctttatttgg aagaagatga acctgattat
    10561 cttcaattaa ctgaatgtga attcatctct gtcagcaaag cttattcatt aaaaaaacaa
    10621 gtattagctt attttaatga ctgcgggatc gaaatcgacc gctactcccc tcgattcact
    10681 gaaatggaaa gacgtctgct gcttcttaat gtgtcgtatc gtttaggcgg ttttaattct
    10741 tgggagctac ctgaatcgtt ttttgaacgt gccgatcgtt tcattgaatc cgtaacggaa
    10801 aatagtggca gattttatga taaagaaaat aaagaaatct tatcgattgg ttttgctatc
    10861 agtttcctac gccagcaagt gtgtgctgtg acaatagatt caaaatttat cgaagaaatc
    10921 aaaaaacgtc ctatttacaa ttatgtcgaa tcagcatggg aaaacactga tttccaaacc
    10981 tattataaaa aagaagaatt cgcctttatt ttgacactat tcaatctttg taattatggt
    11041 ttccattctt atcagttgat tgcggaagac tttcaacagc tacaccaagt gtttattgac
    11101 aatactcccg aaataaaaga actagttgct acctttgaat ctcacttcaa tcaagaatta
    11161 ttcggaaacc aaccgttcga acgtgcgctg attcatctga tgcgttcggc ttgggataac
    11221 tatcaattat tcatgccaga aaaattttat cttttaaatg aggaacagac aaatttatat
    11281 aaagaagtcc agacaatttt ttccagctgg tccagccagt taccttatga tctacggttg
    11341 aatcctaatt gcatgcgggc ttttgtgatc gaattgagcg gaattctccg tctgacaaaa
    11401 gaacatctga ctatctatat cgtcacaaat tcggatgttc attatctgat ttatcgggaa
    11461 gccttggaag ctgtaactac tttcgatttt caagttgcac caacaattta ttcaagtata
    11521 tctgatataa agaagtatgc gcagcagtcc tcaaaccggg tattatgtga acgtacatta
    11581 tatacaccag atgcggtcca atatgaaaat attattccta tttcgatcaa tacgatcgat
    11641 cgagctatca tttcagctgt gcaaaacaaa taaggggctg tgacaaaaat catgtcacag
    11701 cctcttattt gttgaagatg cccatttctg taacaatctc ttgatacacg tcaggctcac
    11761 aacctcactt attttggaca tctacgggtc cgatctcttt atttaaaaaa taagaaatga
    11821 tggttcggat aactaccaac gccgctagtt ggaaaatatc ctgcaaggta ggtttgataa
    11881 tcgattcgat gatcccagct gaaacaagaa tttccaaact tagaagtata tagcctccca
    11941 acatcttttt aatagcattg ttctcgctga ctcggtcgct ttgcactttt gtatggacaa
    12001 ataaatattt caaaaaatcc ttgattgata gacaaacacc ccaaaccaat aaacagatag
    12061 caaagatatc aagtcccagt acaagttttt cgaaatatgg aagaagaaac tgcatcaact
    12121 ctgtcgtcat cctacctctc ctcttctttg tttttctttg tcttctattt cctgattcaa
    12181 gaaataagaa atgactgttc gaatgataat aataagtgca agcttcaaaa tatctgtcca
    12241 agtcggcttg ataactgact cgatgatatc agcaacgact agtacttcca agcttaacaa
    12301 aatataactt gctagcagtt ttcttgtatc tgcattacga gtattacgtt ctgcatagtt
    12361 tttcttgaca tgagaccaat caaaaagttt gaatagaaca agaacgatcc cgacaattaa
    12421 gataccgata gaacaaatat ttaaaatagc aatgatccat tctagaaatt caattaaatt
    12481 tgagcgaaac atatacgcta cctactttct tttaatttgt tcctattctt ttattgtaaa
    12541 agaaagttac cttgttcgtc tatttaaaag ttgttctaat gataacggtt cgcttaaatt
    12601 tttctttttc actctcccat ttttctccct ccatccataa atcgtggaag tatccaaaac
    12661 aacgtagcaa gttaaaataa ggcttagtcc gtcatcagct tttcaaagaa gcactgtctc
    12721 ggtgcttttt ttgtacccaa atttctaaaa atagaaccat acacctcctc aaatcttttc
    12781 ttcttaccta ccctattttt atttacagga aaatcagaca cttcttcttt tttgtcaaaa
    12841 tttccttttt cttctcgtcc tatcatttgg cattctaata aaaacgaact atccttggat
    12901 atagaaagag aaaacaagga ggcgtttttc atgagtaaaa aattaatcaa agtcggcatc
    12961 ggtttaggtt tgcttgccct tggcgctgca tatctaggaa aaaagacagg actattcgaa
    13021 gatgacagtc atttatatga tgaattcgaa tcaatctagc atcctattta gttgaatcac
    13081 tacggacata cttggaagac attggaggaa tcacaatgag aaaaagtaaa gcaataatga
    13141 ttggtgccgg tattgccaat atggcagcgg ctgtttattt gattcaagaa ggcaaatgga
    13201 aaggggatca gatcaccttt tattcattgg atgatcacgg ttcaaatgat ggtgcaccga
    13261 ccgacactgt agtggatgaa tattggaata aaaaccatcc attagaaaac caaaaaggct
    13321 atattgctcg aggcggtcgc atgcttaatt accgtaccta tgtagatttg atggatctgt
    13381 taagtcgaat accttctgtt actgagccag ggatgactgc tgaagaagat acgagagatt
    13441 ttgatgcaaa acaccgaacc tttgacaaag cacgtttgat ggaaggtggc aaaggaatca
    13501 ttgatggcgg aaaactggga ttcaataata aagaccgtat tcttctgact aaactgattg
    13561 ctatgcctga ttcagaagaa gaaaaactag acaatatcac tatcgaagaa tatttcaaag
    13621 atgatccaca cttttttgaa acaaacttct ggtttatgtg ggaaacaaca tttgctttca
    13681 gaacacgtag ttctgctcaa gaattgcgtc gctatatgca tcaaatgatt tatgaattca
    13741 ctcaaatcga acatctagtg ggcgtcaatc gaacacgtta caatcaatac gaaagtatta
    13801 tgcttccttt gatcgagtac ttgaaagaac aaggatgcaa aatcattttg aaccgccgtg
    13861 tcactgactg ggaattcaaa gaaacaccga tgcaagatga aatcactgtg actggtttga
    13921 aaattaccaa tacactgact caagaagaag aatacgttgt ggtcgatgaa gatacagcag
    13981 ttattttcac taatgggtcc attacagatt cagctacttt aggagattac gaaacaccag
    14041 ctgtagaaaa tatggattat ggtgcagctt ctagtctctg gaaaaaagcc agtgaacgtt
    14101 tctacaactt gggaaatcct gacaaattct ttgctgatcg agatgcaagc gaatgggtca
    14161 gctttaccct gacgacgaaa aaccatttgt tgttgaatga aatcacccgt atcacgactc
    14221 aagaaccagg aaatgccttg aattcattta tttcaaccac accgatcacg ccgttaggcc
    14281 aaaaagatgt caatatgtct atcgtcgtgc atcatcaacc gcattttacg tctcagaaac
    14341 caaatgaaac agttatttgg ggttatttcc tttatccaag acgacgtggc gaatttgtgg
    14401 ataaacagta tattaaaatg aatggaaaag aaatgctaga agaactgatt ggacaacttt
    14461 caaaagtaga tcctggacca gtgaatattc gtgaaaaaga aacagaaatt ttcgacagcg
    14521 taataaataa tatccctgtc tatatgcctt atgcttccgc gttgttcaat aatcgtgcca
    14581 aaagcgatcg accaaaagtc attcctaaac attcgaccaa tctggccttt acaggagaat
    14641 ttgtcgagca gccttatcag atgatcttta ctgaacaaag tgctgtacgt tctggagaaa
    14701 tcgctgcttt ccattttgcg ggtattccta tgtcccgtct tgtcaaaaca ccgcgttacg
    14761 acaaagatat tcctactcta gctcgtgccg caaaaaaaat gtttgaataa tcaagaggtt
    14821 gtgaaaaagc tgccctgcat caagtaataa gaacaaccag gctaaaacag cttcttatgt
    14881 ttttgatttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag
    14941 gttgtgaaaa agctgccctg catcaagtaa taagaacacc caagctaaaa ctgcttctta
    15001 tgtttttgac ttggttggac ttattaccgc agatgcaagc ttttgaacac catttaccaa
    15061 gaggttatga aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc
    15121 ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac
    15181 caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt
    15241 tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac
    15301 ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct
    15361 tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa
    15421 atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa
    15481 aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac
    15541 taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg
    15601 gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac
    15661 agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca
    15721 tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt
    15781 tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt
    15841 taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga
    15901 atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca
    15961 ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt catttgttgt
    16021 accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc
    16081 ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc
    16141 tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat
    16201 cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac
    16261 ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg
    16321 cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc
    16381 gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc
    16441 tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta
    16501 cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat
    16561 ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat
    16621 cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa
    16681 agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat
    16741 tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga
    16801 tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc
    16861 actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac
    16921 taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat
    16981 tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa
    17041 gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac
    17101 cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc
    17161 aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt
    17221 tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc
    17281 tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg
    17341 ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag
    17401 accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca
    17461 tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat
    17521 tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa
    17581 aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt
    17641 gatgtaaaaa aaaaacggaa aacaatcact tgttctccgt tttttgatta atcaagcatc
    17701 taaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggta gatagatcaa
    17761 aatagaaaga accaaattga cagcggctag tacaccacct gcaatacttt ttgttgccaa
    17821 tacaccgcca ataattggtg gagttaccca tggaggcata acggtagctg ctggcactaa
    17881 acctgtagaa gtcgcaaagt aagcaacact aactaagacc attggtgtca aaataaatgg
    17941 aatgaacatg atcgggttca acacgatagg taacccaaac atcgttggtt cattaatatt
    18001 gaagacacct ggcgcaatcg acaagtttgt tacaaccata tatggtttgt ttttacgtcc
    18061 tactaaataa atagctaaca gtaaacctaa agttgcacct gttcctccta agttgacaaa
    18121 gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta tgcccagctg cgattgcatt
    18181 aacgtttgct tcaatggctg gcgcattgat tgtttgcatc aaaggatcga ccatattcgc
    18241 tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat gcaagcagta aggcagatgg
    18301 ataagagttt gcaagtccca taaatggttc ttggaccact gtatagaaag aacctacgat
    18361 atcatcaaca ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag agatagtgat
    18421 cattgctggt agtaatgaag caaatgcttt tgcaactgcc ggtggcacac catcaggcat
    18481 tttgatgatc aatttttcat ttccgcttaa acgtacaaag atttctgttg aaataatccc
    18541 aacaataata gcaataaaca aaccgtttga gctcatacct aatgctccgc ctaaagcgaa
    18601 gaaagagcct aaagaaatca cacctgcgga aacaccgtct tttcggtagc cgttagctaa
    18661 atgataagca agcaagaaag caatcaagac agaaagaatg gcaaatgtac cgttccaagc
    18721 agcagccccg aatcctttcc atgattcacc accaaaaatt gagttcatca attcttggaa
    18781 cccgggaatc ggtaagttat tgatcattac tgctaatgct cctaagatca ttaacggcat
    18841 actcaccata aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa tttttgatgc
    18901 tacaggtata aaatgtttct ccataaatgc aacaaattta tccataaatc ttccctcttt
    18961 tctttactgt agattacgcg ttcaatcgac gatacagatc aatgatctct tttgctaaat
    19021 ctgtaaaagc tatagatgtc atcagatgat cttgactgtg gaccgtcaaa agcgtgactt
    19081 caacgtgatt tccttgtgct tcttgtgtca gcatctctgt ttgagagtga tgtgcttgga
    19141 ctaaagattc ctctgcatct ttgatttttt catctgctaa agtgaaatca ccttttttcg
    19201 cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg atcagaccca
    19261 tgatggcttc tagatttttt tgatcttcca tttattgtat cctccgataa tttttattat
    19321 ttgtttatta gtttttctgc ttggtcgagt actttttcac cattcatcat gccatagtca
    19381 gccatattga ttacatctaa aggaatgcct ttaggtgcta gcttttcagc aaattgattt
    19441 tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta catttttaga ttctagttga
    19501 ttatccgcat ctgaagcaga tacagcaaag atgtctgctt ccattccttt tgcttctgct
    19561 gccttttgca tttttgtgac taggagactt gtactcattc cagctgaaca tacaagcatg
    19621 attgttttct tttccatttt aaacacgttc ctttctatac ttacacttgt atataagcaa
    19681 gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat catcacattt aaggtctata
    19741 ccaagttgca tacacactaa aaaagaaagc gcacactaaa cacacaaatt aatttttatt
    19801 accttttttc ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc acaacttttt
    19861 tcaaataaac ggtattcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac
    19921 ataagatgtt gtttttactt tgctattctt attacttgat gcaggacagc tttttcacaa
    19981 cctctcaata aacggtgttc aatcagctga ccctgggtat tagctcaaaa cgctggattc
    20041 acaacctctt ttatttcaaa taaccagtaa atacaatgaa catctacaaa aaacttctat
    20101 ttatttatag atttcgtcag gcaaatccaa ttctctctta tcaatcaaag tctgatacca
    20161 cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc aattcgattc gaactaatcc
    20221 atatcgattc ttaaatgcat tcattggtga cacacagtca gtgaatgccc ataacatata
    20281 gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag taatgcaaat gctgtccgat
    20341 aaattcgata cggtaatcat cttgcacgat tcctttctca tccatatact gttcttcata
    20401 ttctcttccc ataccgtttt ctgttatcag ccaagggata ttatgataat tttcttttag
    20461 atacatggct atatcataca tgattttcgg atagatttcc catcccctgg aattattcat
    20521 tttcttccca ggtaaatcaa agacttcata atatcgttca ggatggaaag gtgtctcttc
    20581 attccattga taacgagggg cgcgaacgcg tttaggataa tattgattga ttccaaggaa
    20641 atctactgta tgatgctgaa tcgtttctaa atcctctgtt gcaggatcga aataaatcga
    20701 ttgtttttga cacaactcca ggaactcttc tgtgtagtat ccttttacca tcggatcaaa
    20761 aaatactcga ttataaaaca aatcatacat ataagcagct ttttggtctt ctggagaatt
    20821 ggatcgagca tagaccattt caggatttaa aatacagcca atccttccac cataatttcc
    20881 gctgcggtaa atctctactg ctttagccgt tgctaaaact ttatgataat tccacatcat
    20941 ccactttttt gtgttttgtt catgcggcca gcgaacagca tccaaatagc aacgcgtttg
    21001 gggaacgatt ggttcgttga atgtaaacca gttttttacc ctatcaccgt aacgatcaaa
    21061 ggcaatttga gcatacttag cataaaaatc aactactttt cttgaagacc agccatcata
    21121 tttttccaaa agaaaagctg ggacttcata atgttccaaa caaatcatcg gttctacgtc
    21181 tgcttcgatc aaagagtcga tcatttcact tatatgctga gcatattctt catctactat
    21241 acagttctca taatcagtaa aaaaacgagc ccaattgata gaagtccgat aatgtgtcag
    21301 atggatggat ttcatcagat ccacatcctg ctggtattgt tccataaaat tagttgctac
    21361 ggctgggcca tacccattat gccaaacaaa atgttcattt ttataccagc tatctataaa
    21421 cgaatcctgt ccttcttttt ttcctttcca accttccgtt tgccaagcag aagatgcagc
    21481 accaagaata aaattttttg gaatagtaat tttcatgatt tcctcctgtt ttttagatcc
    21541 catcattttc cacacgattt gctactttta caaatggcag atagatcaaa atagaaacaa
    21601 taatacaaac aagttgcgta ataactgcac cgatatttcc tgctgttgcc agataagcat
    21661 tgacgatcgg cggcattggc caagggacca ttacaactgc tttcgccgca aatcctaaag
    21721 cggttgcaaa ataaccaatc gttccagtga taagaggcgt taagataaac ggaatagcta
    21781 aaattggatt cagcatgatc ggcataccaa aaataactgg ttcattgatg ttgaagatcc
    21841 caggagcaag ggataacttg gtgatttgtt tcatatcttc ccgttttcca actaacaata
    21901 ctgcaatcag taaaccgatc gtgactccag acccgcccat actcatatac atatcccaaa
    21961 atggcatagt aataatgttc ggtacttctt ttccagcttc aaaagcttcc gtatttacag
    22021 caattgccgc taaaagcaac ggttcccgta tcggcttcac catttgattt ccatggatac
    22081 caatcaccca aaagacttgc gcaataaaca tcaatagcaa aatacccggt aacccttgta
    22141 cgacaccttc taatgggcgt tgtacgacat tgtaaatgat atcataaagg tacattcctg
    22201 taagttcttt gatgataaat cccgcagccg cgatacccga tacagtcaga atcgtcggga
    22261 ataaagcaga aaaactagca gaaacattcg ttggcacggt atcaggcatt ctcagctgta
    22321 gtttcttctg tttcctcaac cagcaataaa gttcaataga aaggatggag accaacattc
    22381 ccaaaaataa ccctttcgta tctgtatatt gtttagccaa tacgttttct actataacag
    22441 tctgattttc attcactaat tcataaacag ttggattaac agataaataa gacatcactg
    22501 cgatgatacc agggaataca ccatccactt tattcagctt ggctaattcg atccctatca
    22561 gaaaaacagc atagatggtc aagaaactta gtgtgacata gctgatggca gatgcgatag
    22621 gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc taagccattt tccgagtcaa
    22681 agaccattgc tgaaaacaat gtgccaaaag cacccgtaat aattaccgga atcaatgcag
    22741 caaatgcgtt ttttatcacc atgatatacc ttaaattatt gattctcgtt gccacgttcc
    22801 ctaaaacatt ggctaattta tccataaaac tcattccaga tcccctcatt cctgcttctt
    22861 ctccttgacg tcatctcttt ttgtctatta gtcagcgctt acatttcgat gaacagaaac
    22921 tatttactcc tcttttttac caaatataaa acaaaaattc atctgatgta tttatcataa
    22981 aatacatcag atgaactgtc aattagtttc tttttctcct attattttct gtaattctct
    23041 ccgatttatt gccatatgga cagtcattgc atcaaacgca gcaagtgcat tccgcctttc
    23101 gatggcctga acgatctctg tatgaatatc gatcgtctcg attttcagat gctggatatc
    23161 ataattttta ttaaacaaag caattgattc attaatgatt gggataatat gcagcaaggc
    23221 tacgtttccg ctagcttctg caatcatcgt atgaaaatcg atgtctaatt gaacatgttc
    23281 ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt aattttttca tttggacgat
    23341 ttgattcttc gttgcatatt ccgctgccaa acctgccatc ttaggctcca ataaatatct
    23401 cagttcaaat aagtctcgta tcatccgatc ctgatcttca atcaaagaaa aaccgagtgg
    23461 gtcattggca atccctcgct tatcactgac aaatgtcccc gctccttgcc gaacttcaag
    23521 gatattcctg gaagctagag cccgcacagc ttctcgcacc gtgcttcgcc ccacctctaa
    23581 ataatcggac aattcatatt cattcggcag tttatctcct acttgcaact gcatttcctt
    23641 taaatatcta aggatcttgt cagtagttac ttctactagg gttttatttt cttccaaacc
    23701 acatacctcc ttttcatctg atgtattttt tcgtaattat tataacacgt ttgaatcatc
    23761 tgatgatttt ttgtatacaa aattgattaa tccgttcata gacatacaaa cgaagaagat
    23821 atttaccata aaaaagacta ggataaa
//
LOCUS       DAZUMI010000033        23810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000033 DAZUMI010000000
VERSION     DAZUMI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.033"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..182)
                     /locus_tag="ACG81C_001139"
     CDS             complement(63..182)
                     /locus_tag="ACG81C_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5064316.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            361..2022
                     /locus_tag="ACG81C_001140"
     CDS             361..2022
                     /locus_tag="ACG81C_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5064317.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2092..2439)
                     /locus_tag="ACG81C_001141"
     CDS             complement(2092..2439)
                     /locus_tag="ACG81C_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5064318.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2523..3464
                     /gene="opp3b"
                     /locus_tag="ACG81C_001142"
     CDS             2523..3464
                     /gene="opp3b"
                     /locus_tag="ACG81C_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5064319.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3465..4508
                     /locus_tag="ACG81C_001143"
     CDS             3465..4508
                     /locus_tag="ACG81C_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064320.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4514..5584
                     /locus_tag="ACG81C_001144"
     CDS             4514..5584
                     /locus_tag="ACG81C_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064321.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5574..6527
                     /locus_tag="ACG81C_001145"
     CDS             5574..6527
                     /locus_tag="ACG81C_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064322.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6539..7549
                     /locus_tag="ACG81C_001146"
     CDS             6539..7549
                     /locus_tag="ACG81C_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5064323.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7834..8655)
                     /locus_tag="ACG81C_001147"
     CDS             complement(7834..8655)
                     /locus_tag="ACG81C_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5064324.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8752..9030)
                     /locus_tag="ACG81C_001148"
     CDS             complement(8752..9030)
                     /locus_tag="ACG81C_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064325.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9298..11307
                     /gene="metG"
                     /locus_tag="ACG81C_001149"
     CDS             9298..11307
                     /gene="metG"
                     /locus_tag="ACG81C_001149"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5064326.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11786..12373)
                     /locus_tag="ACG81C_001150"
     CDS             complement(11786..12373)
                     /locus_tag="ACG81C_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064327.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12548..13870
                     /locus_tag="ACG81C_001151"
     CDS             12548..13870
                     /locus_tag="ACG81C_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5064328.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14025..14801
                     /locus_tag="ACG81C_001152"
     CDS             14025..14801
                     /locus_tag="ACG81C_001152"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5064329.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14794..15363
                     /gene="rnmV"
                     /locus_tag="ACG81C_001153"
     CDS             14794..15363
                     /gene="rnmV"
                     /locus_tag="ACG81C_001153"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5064330.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15356..16240
                     /gene="rsmA"
                     /locus_tag="ACG81C_001154"
     CDS             15356..16240
                     /gene="rsmA"
                     /locus_tag="ACG81C_001154"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5064331.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16691..17320
                     /locus_tag="ACG81C_001155"
     CDS             16691..17320
                     /locus_tag="ACG81C_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064332.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17444..18553)
                     /locus_tag="ACG81C_001156"
     CDS             complement(17444..18553)
                     /locus_tag="ACG81C_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064333.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18762..19184
                     /gene="mgsA"
                     /locus_tag="ACG81C_001157"
     CDS             18762..19184
                     /gene="mgsA"
                     /locus_tag="ACG81C_001157"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5064334.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19371..19469
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19507..20043
                     /locus_tag="ACG81C_001158"
     CDS             19507..20043
                     /locus_tag="ACG81C_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5064335.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20083..21846)
                     /locus_tag="ACG81C_001159"
     CDS             complement(20083..21846)
                     /locus_tag="ACG81C_001159"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064336.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21849..23564)
                     /locus_tag="ACG81C_001160"
     CDS             complement(21849..23564)
                     /locus_tag="ACG81C_001160"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064337.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7680 a         4346 c         4819 g         6965 t
ORIGIN      
        1 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
       61 attcagataa gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt
      121 atctttgtat ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc
      181 atggaaaacc ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa
      241 tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac
      301 tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa
      361 atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt
      421 tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga
      481 ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca
      541 gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa
      601 gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta
      661 acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca
      721 gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct
      781 tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca
      841 gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca
      901 ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg
      961 gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc
     1021 acattagaag attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat
     1081 cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa
     1141 tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt
     1201 gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca
     1261 agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga
     1321 aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga
     1381 tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa
     1441 gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat
     1501 tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct
     1561 gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg
     1621 gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact
     1681 tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc
     1741 tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa
     1801 tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg
     1861 gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac
     1921 caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca
     1981 ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca
     2041 tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa
     2101 caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca
     2161 aaagctgtac ataccgtagc tggcagacga gagcgtggaa aattctggtt ttttctttcg
     2221 gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa
     2281 gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga
     2341 taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag
     2401 tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg
     2461 gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat
     2521 tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt
     2581 ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga
     2641 atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata
     2701 aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa
     2761 tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat
     2821 ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa
     2881 cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat
     2941 taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca
     3001 aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa
     3061 ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg
     3121 aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa
     3181 tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt
     3241 tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta
     3301 tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa
     3361 cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc
     3421 tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa
     3481 agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata
     3541 ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc
     3601 gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt
     3661 tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt
     3721 atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa
     3781 tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt
     3841 tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga
     3901 gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt
     3961 acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg
     4021 aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac
     4081 caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta
     4141 tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat
     4201 taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca
     4261 ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct
     4321 ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt
     4381 acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta
     4441 tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga
     4501 aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac
     4561 gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac
     4621 attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg
     4681 cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat
     4741 cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt
     4801 ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc
     4861 ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct
     4921 taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc
     4981 aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct
     5041 gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct
     5101 gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg
     5161 tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt
     5221 tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg
     5281 ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc
     5341 ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa
     5401 aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac
     5461 ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg
     5521 ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa
     5581 ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag
     5641 tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg
     5701 gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta
     5761 ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt
     5821 tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc
     5881 ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat
     5941 catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa
     6001 gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg
     6061 cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag
     6121 atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat
     6181 tgacctatct atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg
     6241 gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg
     6301 gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg
     6361 agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac
     6421 ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg
     6481 caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat
     6541 gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg
     6601 tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg
     6661 gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc
     6721 attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc
     6781 ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat
     6841 gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt
     6901 aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc
     6961 gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg
     7021 tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc
     7081 gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac
     7141 agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca
     7201 tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga
     7261 tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt
     7321 ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga
     7381 tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta
     7441 taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa
     7501 gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga
     7561 aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct
     7621 aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag
     7681 aaaaagttcg tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat
     7741 acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt
     7801 gaatgattag gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg
     7861 atagattttt gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg
     7921 gagcatagct ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt
     7981 agcaaatact tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg
     8041 cggctcgtgt tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg
     8101 gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc
     8161 cacttctccg ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac
     8221 ttccttggca tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac
     8281 tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat
     8341 attttctttt gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc
     8401 agttgactca tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg
     8461 cccttttctt tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag
     8521 tacgatatcg acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca
     8581 gttaagaaat tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga
     8641 agcaaaacgg ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta
     8701 gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg
     8761 aaaaataaat ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt
     8821 ataccgctct tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa
     8881 acgcaaaatc caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc
     8941 atacatacga atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact
     9001 aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata
     9061 gtctttatta taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa
     9121 caaaacaaaa acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta
     9181 gatagaagtc gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt
     9241 tatggtatac tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg
     9301 gcagaaaagg aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat
     9361 atcggcaact cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg
     9421 ggctttgatg tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa
     9481 gcagaagaac taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag
     9541 aaattatgga aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac
     9601 cataaagcag ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta
     9661 ggtgaatatg aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt
     9721 gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac
     9781 gaagtagaat tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt
     9841 ctattggaat actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa
     9901 atgatcaaca actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt
     9961 acttggggag taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca
    10021 ttatccaatt atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa
    10081 tactggcctg cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat
    10141 tggccaatca tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc
    10201 tggctattga tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa
    10261 atgcttgtgg aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg
    10321 tttgggtcag atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg
    10381 gcaaatgatc tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat
    10441 ggactcgttc ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg
    10501 gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca
    10561 ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca
    10621 tgggtattag caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta
    10681 gctgaatctt tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag
    10741 aagatcttca atcaactagg attggactct gaaacaatga atcttgaagg attacacttt
    10801 ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta
    10861 gatatggaag aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac
    10921 gaagatacag tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa
    10981 atcaaatttg atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa
    11041 aaagtagaag gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac
    11101 cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa
    11161 aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga
    11221 atgatccttt ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc
    11281 atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca
    11341 tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag
    11401 ccaatcatat aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg
    11461 agcttcgctg atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa
    11521 aagcataatg ataaagggtc gggcagattt taattgattc atctggtact cttttttatt
    11581 gtccttacat tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac
    11641 agtagcgttg atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct
    11701 tatatgtgtg attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg
    11761 ttttctttat tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct
    11821 tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga
    11881 aaatcgtacg tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga
    11941 tcaaccctaa aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac
    12001 taagtgccat cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc
    12061 agttctttgt aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga
    12121 taggcggaaa aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac
    12181 ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg
    12241 ttgcaacagt cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt
    12301 gttctttacg ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac
    12361 gcttaaaatc cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat
    12421 aaaaaagaaa tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat
    12481 ggtaaattaa aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag
    12541 gatgggaatg aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt
    12601 tatgggaacc gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac
    12661 tcagtctaca agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc
    12721 tggattgaca gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata
    12781 tattgaagct gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat
    12841 ttactttcct gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt
    12901 attacaatta tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac
    12961 agggttgaat ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt
    13021 taaattaata ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac
    13081 tgtccagtta tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag
    13141 tgcgttgtta gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg
    13201 ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt
    13261 aacagtcgtt tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct
    13321 tgccggtaat ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg
    13381 aaagctggtc actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact
    13441 aactgggatc cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca
    13501 gctgacaaag ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc
    13561 agtagcttgt attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt
    13621 tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc
    13681 gccggaattg ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat
    13741 tctcggtgct attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat
    13801 cggtataggt gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc
    13861 agaagagtaa aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt
    13921 cgtgcttttt ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa
    13981 gtagtgctta tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc
    14041 atacccattt aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac
    14101 aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt
    14161 ctttgcaatt aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag
    14221 aggctggcag ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa
    14281 aggttgtagc attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag
    14341 tccaagataa agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca
    14401 gtattcatac tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc
    14461 gagatatcgg tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc
    14521 ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg
    14581 tccaagaggc tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt
    14641 atctggcacc agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag
    14701 tagaaaagat tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga
    14761 caaatgcgca ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt
    14821 atagtcgttg aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg
    14881 atagaaacaa tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa
    14941 gagactcgtg gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa
    15001 atcattatgg aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa
    15061 ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca
    15121 ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa
    15181 gaccttttag actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta
    15241 ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg
    15301 tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag
    15361 tgaatatcgt gaaattgcga caccctctag aacaaaagag attttattga aacatggttt
    15421 ttcatttaaa aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat
    15481 cgtagaaact gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg
    15541 tgcattgacg gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga
    15601 tcgtttgatc cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca
    15661 tcaagatatt ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct
    15721 tccgttaaaa gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt
    15781 tcttgaatca gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga
    15841 tcgtatctct gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata
    15901 ttatatggaa gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa
    15961 tgtagattca gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga
    16021 cgaaaaagca tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg
    16081 gaataacttg cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag
    16141 cttggaaaca gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt
    16201 cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa
    16261 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    16321 tgtttttcgt ttgtttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
    16381 agaggttgtg ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt
    16441 ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc
    16501 cgaacaagga gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa
    16561 aattcttata cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt
    16621 gacagttatt agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga
    16681 gagagaaaaa atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg
    16741 gatgggggtc attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac
    16801 tttggcgtct catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc
    16861 tgttgcgcta ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg
    16921 agcattgtca catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag
    16981 tatcgtttta aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt
    17041 tgtgatcggt gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat
    17101 tggtaatatg cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat
    17161 ggggattggt gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg
    17221 aacactcagc aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa
    17281 agagtcgatt caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa
    17341 aactctcatc tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag
    17401 agctgcacca acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga
    17461 cttgttctgt ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta
    17521 tttcgcctgg tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag
    17581 tttttgtgta aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca
    17641 ctacggcttc aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa
    17701 taccgaagat aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt
    17761 caggaatacg aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat
    17821 taaagaagtt catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat
    17881 cataaacttc tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc
    17941 ggtcagccat agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa
    18001 gacgctggtg aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat
    18061 ttgataatgg ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg
    18121 ccacacgttg gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca
    18181 gacctaagat ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac
    18241 gtaatttcaa gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt
    18301 tttggaaaac catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc
    18361 cgatatttag ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg
    18421 actttccaca accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat
    18481 taaaatcagt tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag
    18541 ccatttctac cataatacgt tccacacttt cttttttagt tttattaact atttaaagta
    18601 taaatgaaag cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg
    18661 tcaatgtgct agttttaagc tttaataaat atccataaga aataaaaact gatacactgg
    18721 agtaaataag tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg
    18781 ctcatgaccg aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg
    18841 aaaaacatga actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct
    18901 tgccgatcca tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga
    18961 tttcggagga tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc
    19021 atgaaccaga tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta
    19081 cgaatatcgg aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc
    19141 gaagaatcgt ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga
    19201 aaaataacgt ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg
    19261 cgatataata cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag
    19321 taaatgtgaa aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag
    19381 aaaataaagc gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg
    19441 ttcgcggttt tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg
    19501 ctaaaaatga agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt
    19561 gcattgatgg tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt
    19621 gtcacattta ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata
    19681 ggcgcagttt tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt
    19741 ggatttacat tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa
    19801 gagatcactt ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta
    19861 tttttgacac cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt
    19921 tgggcgcccc gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat
    19981 tacgtaggac gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa
    20041 taaaaaacaa gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg
    20101 tgcttgatag agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat
    20161 ttcgacaata gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag
    20221 gcgatgggca atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat
    20281 ttttttctct gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg
    20341 attactgatc atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa
    20401 cccttgaccg ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat
    20461 ataagaggct tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg
    20521 aagattttct cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact
    20581 tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc
    20641 ttttgttgtg tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt
    20701 ttttcctact atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa
    20761 acgctgcttg gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt
    20821 tttgggttcc aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa
    20881 atcaaatgct cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag
    20941 ttcgataaat ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac
    21001 agtgattgac gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata
    21061 agcaaggtga tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg
    21121 ttgtcctttt tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc
    21181 ttgctgaaaa gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat
    21241 atccccaata atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca
    21301 actcacaagg aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag
    21361 acgcatcata aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa
    21421 ggccgcagaa attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt
    21481 tccgtgggag ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc
    21541 ttttcttaat tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca
    21601 ttgactgact acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg
    21661 attgaagcgg acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt
    21721 catcacaact gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt
    21781 tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac
    21841 tttcataact attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca
    21901 agtaattctt catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga
    21961 tgtgcttctt gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa
    22021 gtctgtagcg ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca
    22081 tctaaaatca gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt
    22141 tgtccgccag agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc
    22201 tgacggacaa attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg
    22261 gcgtctttct taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt
    22321 tggggaacaa gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt
    22381 ccgtccatta taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa
    22441 gtcgttttcc cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga
    22501 aatgtaatat tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca
    22561 aaagcgataa ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc
    22621 gaattttctg tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag
    22681 atcacgacga ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc
    22741 aaagcaagaa cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc
    22801 agcaaacaga tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag
    22861 atattggaaa cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct
    22921 gtttcttttt ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta
    22981 acgtgttcat tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt
    23041 ttgatgataa aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct
    23101 acttcccagt cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg
    23161 atcaataaac gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt
    23221 cgggtgatca aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt
    23281 tttttgatta gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag
    23341 tattggcaaa tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc
    23401 atttcaatga tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc
    23461 attaaaggca ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg
    23521 atttgcttac gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta
    23581 tttatttgtc cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa
    23641 tgaaaagaga acaagccttt tcataaatga aagttttatt atgaagaagc tttagaaatt
    23701 gaatagacta ttttttttaa aaaaaagaaa atgagaaaac atgccagtct cttcattcat
    23761 aggaacgtct attttactta atttttctat aaatgaattc tgcataaaaa
//
LOCUS       DAZUMI010000034        23653 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000034 DAZUMI010000000
VERSION     DAZUMI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.034"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACG81C_001161"
     CDS             complement(26..601)
                     /locus_tag="ACG81C_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5064338.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACG81C_001162"
     CDS             complement(606..1580)
                     /locus_tag="ACG81C_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS5064339.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACG81C_001163"
     CDS             1863..2831
                     /locus_tag="ACG81C_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5064340.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACG81C_001164"
     CDS             2928..3221
                     /locus_tag="ACG81C_001164"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064341.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACG81C_001165"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACG81C_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5064342.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACG81C_001166"
     CDS             3678..4433
                     /locus_tag="ACG81C_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5064343.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACG81C_001167"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACG81C_001167"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5064344.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACG81C_001168"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACG81C_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5064345.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACG81C_001169"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACG81C_001169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5064346.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACG81C_001170"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACG81C_001170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5064347.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACG81C_001171"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACG81C_001171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5064348.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACG81C_001172"
     CDS             9170..9712
                     /locus_tag="ACG81C_001172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5064349.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACG81C_001173"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACG81C_001173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5064350.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACG81C_001174"
     CDS             11305..12207
                     /locus_tag="ACG81C_001174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5064351.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACG81C_001175"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACG81C_001175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5064352.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACG81C_001176"
     CDS             13650..14072
                     /locus_tag="ACG81C_001176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5064353.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACG81C_001177"
     CDS             14541..14771
                     /locus_tag="ACG81C_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5064354.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACG81C_001178"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACG81C_001178"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5064355.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACG81C_001179"
     CDS             16182..16358
                     /locus_tag="ACG81C_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5064356.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACG81C_001180"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACG81C_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5064357.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACG81C_001181"
     CDS             16853..17320
                     /locus_tag="ACG81C_001181"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064358.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACG81C_001182"
     CDS             17405..18289
                     /locus_tag="ACG81C_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5064359.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACG81C_001183"
     CDS             18403..18639
                     /locus_tag="ACG81C_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5064360.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACG81C_001184"
     tRNA            18727..18807
                     /locus_tag="ACG81C_001184"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACG81C_001185"
     tRNA            18814..18885
                     /locus_tag="ACG81C_001185"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACG81C_001186"
     CDS             19011..19181
                     /locus_tag="ACG81C_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064361.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACG81C_001187"
     CDS             19401..20108
                     /locus_tag="ACG81C_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5064362.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACG81C_001188"
     CDS             complement(20167..20811)
                     /locus_tag="ACG81C_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5064363.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACG81C_001189"
     CDS             20938..21225
                     /locus_tag="ACG81C_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064364.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACG81C_001190"
     CDS             21333..21653
                     /locus_tag="ACG81C_001190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5064365.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACG81C_001191"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACG81C_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23463
                     /locus_tag="ACG81C_001192"
     CDS             23044..23463
                     /locus_tag="ACG81C_001192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064366.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DAZUMI010000035        23379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000035 DAZUMI010000000
VERSION     DAZUMI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.035"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..348)
                     /locus_tag="ACG81C_001193"
     CDS             complement(<1..348)
                     /locus_tag="ACG81C_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064367.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMY"
     gene            complement(355..903)
                     /locus_tag="ACG81C_001194"
     CDS             complement(355..903)
                     /locus_tag="ACG81C_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5064368.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(948..1280)
                     /locus_tag="ACG81C_001195"
     CDS             complement(948..1280)
                     /locus_tag="ACG81C_001195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064369.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1294..1551)
                     /locus_tag="ACG81C_001196"
     CDS             complement(1294..1551)
                     /locus_tag="ACG81C_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064370.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1746..2288)
                     /locus_tag="ACG81C_001197"
     CDS             complement(1746..2288)
                     /locus_tag="ACG81C_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS5064371.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2309..3400)
                     /locus_tag="ACG81C_001198"
     CDS             complement(2309..3400)
                     /locus_tag="ACG81C_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5064372.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3408..5618)
                     /locus_tag="ACG81C_001199"
     CDS             complement(3408..5618)
                     /locus_tag="ACG81C_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5064373.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5634..8162)
                     /locus_tag="ACG81C_001200"
     CDS             complement(5634..8162)
                     /locus_tag="ACG81C_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5064374.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8214..8624)
                     /locus_tag="ACG81C_001201"
     CDS             complement(8214..8624)
                     /locus_tag="ACG81C_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS5064375.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8637..8855)
                     /locus_tag="ACG81C_001202"
     CDS             complement(8637..8855)
                     /locus_tag="ACG81C_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFS5064376.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8852..9844)
                     /locus_tag="ACG81C_001203"
     CDS             complement(8852..9844)
                     /locus_tag="ACG81C_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5064377.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9923..10834)
                     /locus_tag="ACG81C_001204"
     CDS             complement(9923..10834)
                     /locus_tag="ACG81C_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5064378.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10853..11197)
                     /locus_tag="ACG81C_001205"
     CDS             complement(10853..11197)
                     /locus_tag="ACG81C_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064379.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11217..11495)
                     /locus_tag="ACG81C_001206"
     CDS             complement(11217..11495)
                     /locus_tag="ACG81C_001206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064380.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11854..12354)
                     /locus_tag="ACG81C_001207"
     CDS             complement(11854..12354)
                     /locus_tag="ACG81C_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064381.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12355..12900)
                     /locus_tag="ACG81C_001208"
     CDS             complement(12355..12900)
                     /locus_tag="ACG81C_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064382.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12897..14153)
                     /locus_tag="ACG81C_001209"
     CDS             complement(12897..14153)
                     /locus_tag="ACG81C_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5064383.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14514..14825)
                     /locus_tag="ACG81C_001210"
     CDS             complement(14514..14825)
                     /locus_tag="ACG81C_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064384.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14848..16323)
                     /locus_tag="ACG81C_001211"
     CDS             complement(14848..16323)
                     /locus_tag="ACG81C_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS5064385.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16772..17224)
                     /locus_tag="ACG81C_001212"
     CDS             complement(16772..17224)
                     /locus_tag="ACG81C_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064386.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17226..17591)
                     /locus_tag="ACG81C_001213"
     CDS             complement(17226..17591)
                     /locus_tag="ACG81C_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064387.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17690..21973)
                     /locus_tag="ACG81C_001214"
     CDS             complement(17690..21973)
                     /locus_tag="ACG81C_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS5064388.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22120..22359)
                     /locus_tag="ACG81C_001215"
     CDS             complement(22120..22359)
                     /locus_tag="ACG81C_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064389.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22449..22691)
                     /locus_tag="ACG81C_001216"
                     /pseudo
     CDS             complement(22449..22691)
                     /locus_tag="ACG81C_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22804..23040)
                     /locus_tag="ACG81C_001217"
     CDS             complement(22804..23040)
                     /locus_tag="ACG81C_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064390.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6892 a         5044 c         3424 g         8019 t
ORIGIN      
        1 atacattctt ttccagttac atttaatttt taaacaaaat tctccccaaa aaggaacttt
       61 ttctagtgaa aacttggtaa tctctttgta attaattctt gttaaagaat aattaaacac
      121 ttcattttta cttgttattt tttcattgac tataactagt tcatctggta aaaaaattaa
      181 ccaacaaaca tcgacaaaca gtaaatgata taatccattt ccacctccaa gcatattcaa
      241 tactggtgaa catgccttag aaacacgaat atagttatgg tttaaataga tattttcttt
      301 aaacattgct tgtttaaaag ccatatgctc ttgttctaaa cttttcataa acttttactc
      361 taatgcacag ctagcagcag ctgctgcaat tcctgctaca atatttacct tagctaactc
      421 tttagctgca gcttttccta attgtttttc tgcaattttt aataataaat ccgcagcttt
      481 ttcaaaatta aaagctttta atgcttcaat aaaatctccc tgcgtaccta tagcccaagc
      541 cactcctcct ccaggaagag aattaatggc tacacatcca gcatatcgtt tccaatcaac
      601 tttctccatt ctaggaacaa taatatgttc ataaaagact cttgctgctc ttccttgttc
      661 gttatcttga ctgattcgtt cacgaatagc accaaaatcc tttatatggt atacaccatg
      721 ttcgtctaaa aaaccaactg tatcaaaata aaaagttaat tcatctacta atttattctt
      781 tatttctaaa tcaactgtat tcactttttc tgtggcatac acacttgttc cagtactaac
      841 aaaagtactt aaacctaata agaaaacacc taaaacacca aatattttaa ttattctttt
      901 cataatttct cctccataaa aaataagtta ctttttaaat atcttgttta tgaattcatt
      961 ccccaaaggc cttttaagat tgatacagca gtccgttcac ttcttttaga cttgtcatac
     1021 aagtctttta aatgatccca tatcagacta gtttgtctat ctagcttcaa tccattaaac
     1081 gctaatgaac ggttcatttt ttctattact ttagataaag ctacagaaat ttttatacct
     1141 tcacaacgcg catcttggat ttcaataata gtatcatcaa ttagtttttg aatttcttgc
     1201 ctatcaacat attcaatctt agaaagagaa tcttttaaag ctactaatga ttcaacgatt
     1261 tcttttttct cttcagacat tttcttcctc cttctagtga cccgtaaagc catgtagagc
     1321 agagtccatt gaaccagtaa ttacactatc tacaacgcca ttccaattta ctctacattt
     1381 tccatttttc agatttgtcg gtccatacgg atagcctttt tgacattcaa tgttcggatt
     1441 ataccagata ggattatacc attcagccgc agaagcagat tcagtcgata caaacaatcc
     1501 cacaccaaca aatccaatac acatcacact atagccaatt agttttttca tctcgtatcc
     1561 atctcctaaa aaatagaata gttcttatag ttattcataa gacatatttt ttccgttttt
     1621 gttttatcct ttctgtccta taacaacttt tttcaagtaa agaatgacat agtttttctt
     1681 aaaaaaacag tctgtttttt tctatttctt cggtgtataa taaatatgag tgttcatttt
     1741 ttattttaat acgcactccg tacataataa atcttgatcg catcaatctt ttgagcttct
     1801 tgatctactt tcaattgaag taatacaaat tgagtaacct tattcattgt ttcttctcca
     1861 aataacaaat attgatccgg attctctata tcttgtgtca cctggtaaag tttcccagtt
     1921 gttttcattt gtacatgcgg gtctacatca atactattgt ctttaataca acgatctgtc
     1981 atatagtccc tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta
     2041 gcaaaatctt ttaagaaatt ttttgtcatt tcatctattt gcatttgttg cgctgtctcg
     2101 tcttctaatg aaccaatttg actgttactt tcagtacttt gaagagttcc acttgtttct
     2161 tttactgctt cgattgtttc ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa
     2221 aaccaatgac aaccagtaag acctatcaaa cataaccaac taaatactaa aattatccct
     2281 tttttcacgt ttctagtcac tgcctttctt atttcacctt acgaaccaga ctacgaattt
     2341 ttgtttgatt gtacgtgcgt tgataccgag ccataatccg tccttgctcg gcattctgtt
     2401 ccacactaga gaaatgttgt ccgccatatg aaacatctgt tataattccg gtgtgaccat
     2461 agattccagg agacaatgca ccacctgctt gccaattcac cacatctcct gcttgtaaat
     2521 cacttggttt tgggttcatg attacttgcc aaccataggt tgtccagtta taatcttccc
     2581 ctattaacat cgcaaacatg tgtccagaac ccattaactg tgggccacct aattgagata
     2641 cataccatgc cgtaccacca taacattccc cattatttac ggatcgtcca atgatccgat
     2701 ccaaaacctt cgcattgcct ttactttttc ctgcaataag cgaagttttt tttgttggta
     2761 ggctattcca atgcttgatc gcaaaagtat catattccgt taagttatag gacgttatga
     2821 tactcattgc tcctcgataa tacggactcc cttcggtaat acctgctgcc gcatagcctc
     2881 cacgacctag aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt
     2941 tgctttggct cgccaccata aagccataat agtcataaca agcttttaag ttcggaaact
     3001 tcatatagaa accaccttca gaaccaccaa ttccacgtgc agaaccttgt gggtagggac
     3061 aaccaggaaa ccaggtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc
     3121 cataagctga ttcatagcct aattgagaaa gaatcacact cggtaataac tgatacttta
     3181 cagctgcacc aactgctgct tgcgcatgtt catctgtgat tggaccacgt cctttaatct
     3241 ctgaaaaaat cggtaatcct tcggtatatt ccccattaaa agcgacagct agacctgtag
     3301 cttctcctcc atgctcagaa agttctcctg aaaaaaagaa tggcaataga aaaaagggaa
     3361 gaataaagaa aagaagacaa cctagacacc ctttcttcat ttcttcctca tcctttcttc
     3421 gataatggtt tttggactgt tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg
     3481 tccaaaatcg gcagtgtttt aggatccttt tctatttttt ccgattgctc tgttggctta
     3541 tctaaagaag aaaatgcagg ttgtaacgat tttggtgcgg aaatcgacgg tttactttca
     3601 gacgaacgtt gccccggttg ttgcacagaa cgatttcctg atagagtggc taccgacgaa
     3661 ttaggaacag agtattggtt caataactgc tgcattgctg gataactccc atttttcgcc
     3721 tgatttttca cttggcgaat ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg
     3781 ggtacttttc cagaaggcaa cggaatcgat tgaccaacaa aagcgatacc tttatttaac
     3841 aatggttttc tttgattatt cccttgttgc ttactcaatt gtgccaatga ttgtacctgt
     3901 tgttttggtc ttactgaatt catggacgaa tttggaatca aaccatttga aagaccagaa
     3961 ttccactctg ttttcgattt ggtagaagac atttcaggct ctaccccatg ataacgaaca
     4021 tgacgaccac tataaggcaa ggcttgattt gaacgcccac ctagttcaaa gggattaacc
     4081 acttgcctcc cttttaccga ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca
     4141 tgtatcgctg cagaagcgag tggattcgtt gctcgataca cattccctgc taatcctgca
     4201 cctttgattg ccaaattttg tccagcagtt gatagttgac caggcagttg tttcatttga
     4261 gacaaagaat gacttgctat gtttttcgtg ttgtttatag tttgatctgc agctcctaac
     4321 cctagtttct ttaataaatc tttccgtttt acaatgagta aaattgatag gagtgctttg
     4381 acaaacatat ttcctaaaaa accaaaaaca gtattcattg gaattaaatc gtctactatg
     4441 ttatagatca aaaatgccac caaaataatc acactgtaaa aagatttttg aaacatcaag
     4501 cccaaaaagg actttaaggt attcatgagc atataatcaa atactggaac aaaagaagcg
     4561 agtaatgaaa aaggaatcac aagtaataaa cacaacacca acatttgaaa gataaaacga
     4621 atcatgccaa tcactacaag tggtaatcca atacttagat taagcaatgg caccatagaa
     4681 ccaacgactg ctttataaac agctttagtt ggttgaatat agacacgata agccttcttt
     4741 ttccctctat cttctcctgc agcagtggtc acttttgtct caatatcaga taattcttca
     4801 cttgacgctt ccttggctag aaattcttgt ggttcaatct ctgcttgata caattcttct
     4861 aaatttgttg tcccataatt taacaatata tacggatcaa caacagccgc tttataataa
     4921 agttggcgca ctttttgaat ggcttccttt tgtgtcgttg gcactaaaac accattgaca
     4981 tcttttgtcg ccaattcatc tagactattt gtagcagaaa aaataaagcc ttctacctcc
     5041 acggacaaat catttaaccc tttggtaaaa gcttctcctt tactaggtac gttactacca
     5101 tagccaaacc aaaccattga aaaaatgaca acgaagagaa atcgaataaa caccattttt
     5161 gcgtaatgtt tccctctaaa tatacgaaga gtaaatagat ataacacata caagacgaca
     5221 atccccgcac caataggatc gtatattttt gaataaatag tatccgaaaa agtgaaaaac
     5281 tggtgaatta actcgttcat tgcagctcct tcatagagct tctcaatgac aaaatcaagg
     5341 ccttgccaaa tcaatttact taaactaaag aacatattcg tcatcatttt tagaaaatct
     5401 gtgccatttg ccccaaataa cattttgcct aatgaaaatt cactatcaga taaataagaa
     5461 gaaaaggtat caatatgaaa tttcccaatc tcatagatcg attgatccac ctgcttttct
     5521 gccgcataca ccaatccacc aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa
     5581 acgcaccatt tcttttcctt atgctttata taagccattg tttcacctca tttctattgc
     5641 atgattaacg tatcctgttc ttctacttga gagtgacttg ttttttccgt tgtttgtaat
     5701 gcagcggtca tttcttcaaa caaactgtgt acattgagtt ttcctacacg tccataggga
     5761 tctaacatcc aacactgtcc tttaatcatg tggctcattt cagctaagtt cgcttcattt
     5821 acttccaaac gtaaatgatt caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag
     5881 acgacaccaa aattcccttt ttctttatct gaacctaagt caccaataaa ttgtgtatct
     5941 aacactaatt gattcatctg ggaacgcccc acacgtgcca ttgctgcaat aatactacgt
     6001 cctgcgttcg aattattaaa tacccatgct tctgtaaaat aaatcgctgt tttcttacta
     6061 ctatctcgtt ttccaaacat ttcacaaaaa cgacctaaac taatcatcaa acacaaagac
     6121 tttcgatccg catctgtata tagttctggt cggacattgt catttggtaa tttcaaatct
     6181 tggatctcta aaatcgttgt tttggcatta aagtctaatc ccgggttttc tccataagaa
     6241 aatcctaaac gtaaaatcga atcttctacc gtcaagcgta aaaactcacc atattctcga
     6301 acctcttttt gttccatttg ttctaaatag tcgaccacat gcatcaagcc aacttgttgt
     6361 ccggcttctt tttgaactaa aacttcttgg attcccttta ataccgcccc ttttaaaagg
     6421 tgattggtac ctaacggact taactcatca atcatatcct gggctacttg tttcgcatct
     6481 actcgggata aacaaacaat tggatctaat acaccataat tacggggatt tgtagcatct
     6541 aacgtgacat aactcatttt ttgcaaatgt tctacaaaca aagggtaatg tttctgataa
     6601 tacgggtctt gaatgagttt cgtaaaccat ttgcgctttt ctcctttggg atccacaaat
     6661 aaactttgta cattcataaa cgaactatac aaaaagagca agccaactaa aaaggtcttc
     6721 cctttaccgg tttttccagt tactgctaca tgaggcgcat caaacaacgc acctttgatt
     6781 ccttccgcaa tggctagaag attcaaaaag acaaaacgat ttgacgcata aattaattct
     6841 tctaatggtg ttttttctcc tcgagaacca ccatccccta atgtatcttg ccgaccaata
     6901 taccagccgg attccatacc taattctgat actgctgaaa acaaccattc gccaactgct
     6961 tctacagttg ttcggtgctt ccaatgtaac tgccgtccca atggttgccc taataaacat
     7021 ttatagaaca attctcgttg atcatttttt gcacggtgga tatggataat cgtatgaagt
     7081 tgttgaataa ccgttgcact attttgacga acttcttcaa ccgtttgacc gtatacaata
     7141 aaacatgcga tccaatcgac gacaggttgc ccattatcta ctcgttgctt taaatgtttt
     7201 actaatcctc gaacagttaa taagcgttca ctaggttctt catcattttg aaccaattcc
     7261 ttatgctctg ttttgaaccg ttccgatagc caattcagtt ttaaaccaat acctccctgt
     7321 cctttcaagc gtggatagtg aattttcatt cgcaattctg ctgggaaaga aagatcctga
     7381 gcaaattgaa atagatcaca atttctcata ttttcaggtg tttccccaat aggcaacaat
     7441 gctaaataac tactccctaa ctcatcttct aattgaagaa ggcctttatg ttccttcggt
     7501 atacgtacat acgttccttg attgtctttc agtacttccc aattattttg ttcttctact
     7561 ttgtgagcca ttcctcgaat atattgcaga cgattgagat acaacagctc tttttttact
     7621 aagggacgaa tagaaaagct ataaagtaat tgtgtcactt cttttacata aggatcactt
     7681 tcagaaaaca aagtttccaa atcatctact tctaatccaa ataagcgtgc taaacttcct
     7741 gctaaatcta accagattag atttattaca tcatgaaaac tctccaccgt tgctttttct
     7801 ttgactttga ttcctaaaat aaatgaagga cgatagactt gaccttctcg ttccaacaat
     7861 gtcactgttt cttttgcata atacgcacca attgcttgac tatcgggaga aaatcccttt
     7921 cctaatgtgt caaacttcgt ccgtaaattc attgttcgtg gatacatcac caactccaca
     7981 tcttcataat ctgctaaaag caacagtaaa cgagccatca tttcttttat ttcctgtgtc
     8041 ttttttggtg tgttttttga aattgttcgt ggaaaaacat gatagtaggc ccagatacaa
     8101 tcctctccat ttaacaaaca atttttcttg aatgctttta ctggttcttt tacaagcacc
     8161 aatgaatttc acctcccaat aaaaaaggaa caaccagttg attgtccctt tttctaatcc
     8221 tctaaataaa catacttatt cacaaaaagt aattcttcca atgcattgtt tgataccttg
     8281 tcccagttat aatacacaca atcaccattc atcatacgaa tcatataaca aaaccaatcc
     8341 cataaataaa aatgcacttt ttttccatca ggttttagtt ttgccagtgc taacacacat
     8401 aaatagggga gtcctatata aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa
     8461 ccaataaatg aacgtaaaaa taaatattca aagattacga ccaacaaaaa agttgctgca
     8521 ttgactaatg caactggaaa tggaagacgc caattgcttg taatctgttg tacgcgataa
     8581 ggttccgcat aaacaatcga ataatcataa ggttcccaat ccacttcact atcctcctaa
     8641 tccataaata atttttcaat gattttacca aacgcatcaa atacacgtgt tggatcattt
     8701 aagaagaaat aaactgcggc acctgctgcg atagatatta atgcatttcc tacttttgat
     8761 ttcataaaat ttcgcaatcc cagataaaca accaccatta ttaaaaaata gccaccttgc
     8821 gttaatataa aatctacaat tcccttcagt gtcatttccg ttcttctcct ttcctatcca
     8881 accaattatg tgttaatttt tcaacataat actgatttgt ttttttcgta atgacaatcg
     8941 tcatttgttc tttttgtaaa agtgacgtat caccatctcg aaagacgacc gttgctttta
     9001 caataaaagg ttttttcgtt tctttataac gcaatacttt catctctact tgatcaactt
     9061 tagctgtttg attgagtcct tcaggttctt tcatcatgta tgacatatct tttgcattgt
     9121 ttttcgcata cttatctaag aaatcttcca caaatccttc aacatctttc gcttccgacg
     9181 catcaaccgt ttcgtataat gtgtcatcat aagtaatcat tcgttgtttt tctgtattct
     9241 ttggtagtgc cgtaaaataa ggataagcaa taatacgaca gcccttgttt gaaatagaaa
     9301 agggaagatt gagtacttgt gtgattgtca cacttgtttc tttcttactc tctggattca
     9361 tcgagcgaat atcataagtt actttataac tagcagttgg aatggtttca atttctttta
     9421 atccaataaa cgaagcagca gttaattttc gaacacctga aacacttcct ggttcttgaa
     9481 taaagttttg tgcataaaac tcttttttta atgtttcttg acgttgttgt tcttgttcat
     9541 gattatcgaa tgtattcata tacaaagcaa caaaacgatt cataaaggat tcaatttcag
     9601 gtgtgtacgt taattctgac cgactttgta cttctttctt ttgtaattgt tggatttcat
     9661 ctttcaaaaa cgacacttga accttcgttt tcatggattg aaaaaatgcc aagccccctg
     9721 aaaaacaaag aaataaaacc aaacaccaaa tccatttcac tgcttttttt gaatggattt
     9781 tcttttgctt cggtactttc tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt
     9841 tcataagcta ctccttctag tatgttttta ctagtacgta cgattgacaa cgataagctt
     9901 gtttttctga aaaatagtcc attcaattac cctcattttc tattcctttt ctctttcttt
     9961 tatatccaac aaatccagca atacttgtca acactcctcc tagaacagaa agtgcaacat
    10021 gtatttcttc accagtcttt ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa
    10081 tattgccact tggaagtttc tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac
    10141 tatcttggat taatggaata ccatctttta caccaacaat tgtttgatca cctgctactt
    10201 ttaatggggt ggttggtgtt ggaatatggg tcaattcctc tacattacta gtgacaatcg
    10261 gtacactcat tccttgagaa gtattcgtcg taatttcacc gcttggggtt accgatactg
    10321 tgacttcttt tgctggaaca ataggtttat tttcttttgg aattgcttct gatttttcaa
    10381 caggttgttt cggttcttct ttttgttctt cactggtaga cgacatcgtt ggctgttccg
    10441 tacttgacga atcagtattc ttttcagaag aactggttgt tcctgattgt tccggagttg
    10501 tgactatact tgaatccgtt gttgttgaac tatgcacacc ttcttcttcg atcgaatcag
    10561 tcggtaaagt tgattcagga aactctggtt ctggtgtcac tggctctttt tcaggtttct
    10621 ctgactgcaa ttgttcaggt tctgctggtt tttccgttgt tcctccattt agctctgtct
    10681 caacatctgg taatccttcc cctggcaatg tttctacaat ttctgactta ggtgtttcta
    10741 cagtttcatc tgcataaacc aatgaactcc ctaattgaaa cgaaaataat gaaattccta
    10801 gtgttgttac gactgctaat gttgtttttt tcatgattat tcctccaatt ttttatgcaa
    10861 ttcgtgtttt gttcatagac gccgcactaa tattatctga acgataaaag aactcccgtc
    10921 cgttttcttc taacccaaca tatggattat ttttgaataa ttgaatcaat agttcactgg
    10981 tgttgtattt tggatagttt gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt
    11041 caacaagtaa ttcataacga taccgcttca atccttttcg ttgttttaaa ggatctctat
    11101 accaacttgt ccatcgttcc attcgtggat agagatagac acgatacaca tagaacaatg
    11161 ccataccaca taccaccaaa aataataatc gccacataaa aattccccct tttcttctac
    11221 ctttcttgaa tcatcgaaca ttcataccct ctatagagat ttgaaaaatc catcatcaat
    11281 tgttcatata aacgttgttg caaatagcga ttgtttcgaa tacataattc ttcatagcca
    11341 tcaatcactt gatcttcttt ttcatagcat cttgtagctc tatgacgctc agcttgcaag
    11401 acataaccaa actctgcacc atatcgttct actgttaata actgttgact acgcaataat
    11461 gcaatataac aatcacaaaa atgttcaacc gacatttgat ttttctcacg tttgtgaaat
    11521 aggtattctt tagaaagcac attattcgtt aaacaatctg ataaatagcg ataatctggc
    11581 ggtcggttat tttcatctgt gaatttttcc cataataaac tgatttttat tcgttcagcg
    11641 gctagatagt cttgttcgcg tggataaaat tcatcaatcg cacaaagttt tttatcacct
    11701 gaataaacaa atccatataa tgcgaaacga actcttcctt ttcaattggc tctcctaaaa
    11761 attccagtga cataccatct tctccttcct aaaaaaaata aaaaagcaga aaccgatttg
    11821 gcttctactt ttctttcctt ttatagctct tttttacgga agaatccgca aatgacattt
    11881 tttcaaaaaa tcaatcggca caagttcacg aaaacttgga gcaattttgg atagaatcac
    11941 ttccccatct tgtaaaatac aatcataaaa atcatcagtt acttgatact tacgcttctt
    12001 acaataattg ataaaatagt agcgatcatt ttctatttcc attcgttgtt ctactaagcg
    12061 catacgaaca tcatcataga aataagccac aaatcgccct tcaagttcat gtttttcaat
    12121 gggatactct tctggtaaca atctattttt tcgtcgttta tacattgctg gataccttag
    12181 aagaagttca tctaaacgtc tgtcagtata tggtaactga cgaatcaacg attctaatcg
    12241 ttctttatac agatagaata attggcgcaa gcaatagtgt accgcctctg gattttctaa
    12301 tactgttgga tcacgtgacc aatacgcacc aacataaatc ataatttttt tcatttagac
    12361 aatcacacca gcaatactaa tgaccgatcc acaataggta gaacaagtgg catagcgacc
    12421 aatttcctct aattctatct caatctcttc taaagaaaga tatgtctgtt cttctgttgg
    12481 ctggcgactc atttcccaag tgacagtcac caatgaaatt tcttccataa gaggtttttc
    12541 gctgctaagt gctgccacac agttttcata tatcttcatt ttatcgcagc cttttttaac
    12601 cgctaaaatc gcaggtattg gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc
    12661 tttccttcgg ctaaattcca actcttcgag ttcttgttcg tatcccttac gaacttctaa
    12721 taagcgtgca atttcttgcc agttctcttt ttcctgataa cgtccagctt ttaccgcttg
    12781 ttcaatataa ggaactcgaa aacgaaaata agacaaatca accatctttt catttaatgc
    12841 ttcgattgca gcctttaaat ccaacaggcg cagccattct gttgtcgtat atgcattcat
    12901 gcaattttcc ctcctgacgt tcccatccat tctagatagt tttgttgctc gtcatttagt
    12961 tcggcattat caatcccttc ttgtacaaag tctcttccta aaatacgatc tgcttcttgt
    13021 aacaacttta atgtcggagc tacttgtttc aaaaaccagc gttctgaacg atctaatgag
    13081 aatttttctg gctttactgt aaaatccaat tcttccatcg aacgaaataa ttttgcccat
    13141 cgtttatcaa caatccgtgc atcactttcc agtctatccc aaacaataag ttttgcatta
    13201 attaaaccaa cgccaatctc tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct
    13261 actgcttgtg ttgctttctc attggctaac cgtaactcat atcgattata aataccatat
    13321 ttcgttaatg cttctaattc tgaaattttt tctcggttcg caatttcaaa acgtttctca
    13381 taaaaattaa aaaagagagg actctttcgc gatccaaaat acaaagataa tccttcactt
    13441 cgaaaatagc catcatctaa aaaacaaccg ccaatattct ggaatctctt aaagtccatt
    13501 tctaataaac ctgctttttg tttttctagt aattcaaaga gatcgaaatg ttcttccgtt
    13561 tccaatcccc aaagttcatc taacgcaata tcgaatcggc tacattgcac acgtgtataa
    13621 ccagcttcca actttccttg atttttccaa atggaatata gccatgttcg ccaatctgtt
    13681 tgttgttcac gcatgacttc ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt
    13741 acattttctg ttacagttcg ttttgaatgc cagaacaccc agatattccc acgaacagaa
    13801 agacagtcaa aaagatacaa gcccgttgga cgtgtttcaa attctgtcag atccattcgt
    13861 aatacacgtt gaatcactgt tttgtagtct gtcccaaaaa aggaaagacg caaataatca
    13921 atcattgctc gaagatctat tccgcttttc atatacaatc gaaagcgttc cttgattcgt
    13981 tgttcaaaat cctctgggat ttttcttttc ccttgttcca tctttctaat gtaatctttt
    14041 gagcagccca tcaattgcgc aaatcttgct tgtgatagcg gctttttatc tccacttata
    14101 ttatgaattg cacttaactt ctttctgtag ctttttagct caatcggtgt cattttctcg
    14161 ctccttttat aaaataacaa aatcgatttt ttttaaccct ttagaaacac cgatatatca
    14221 actagtcccc ctcgtttttt gggaaaagta gtacaactgc tgaccttaga taatcacttg
    14281 atacaaaagg gtttataaaa cttttttcta tgtgttatgt tatttctgcc ccccatatta
    14341 tatatcgggg ggctttacac aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg
    14401 cacacgctgc tagcgagtgc gcattatagg cccatgcttc gcagccttaa tgcgcaccgc
    14461 caggcagagt gcggctacca gactacgtct agagccctcc cgatagataa taactacaaa
    14521 attaaatgat ctaattttgt tatttcacta tatttttcag gagaaaaaac gcgataaatt
    14581 gacaaagtta aatcctcttc taaatctact cgataatgat acaatcgagt caatcgttct
    14641 atatcttttt tcgttagcaa tacttgatta ataacttttc ctgtaaaaac acgttctttg
    14701 cgcaaaaagc ggtaatcagt tacttgttca atacgattca accaatcata aaacgttctg
    14761 gaagaaagag aaggattaat cattcgtatt gctgtagcta ctctataata acaactctct
    14821 gccatttaac ttcacctcct tagttgatta caaaatatgc gcatcagaaa aacttgttcc
    14881 ttttggatct tttgctaaat acatttggcg ctctagttgt tcttgtaaca actgattatc
    14941 atctcccatc gtttgatctt gactatcctc ttttactgta aaatcataga caatcatttc
    15001 agtaaatttc tcaaattctt tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa
    15061 ctctacaggt acatcaaatc ctggaaaagc atatccacga ccaggttgat ctttgttgta
    15121 aaatggctta tttttaaaat cttctccaaa agtcattaaa taaccaatgg gagacaatcg
    15181 accaacagaa atacggaatg ttaaattatc ccgaacacca cttggaaaat attctgcatc
    15241 tggtcgttgc attgctaaaa taatgtacat ccctgcttga cgacccttta atactaatgg
    15301 ctgaatcaat ttatcgactc tatttctcgc ttccatatcc aacgaaccat aaaaagctgc
    15361 ccattcatca aaaaagataa aatgcggtgg taacccataa taagcataat ttttccctgg
    15421 ttcatattta ggatctttca tggttttcat ataggcatac cgttgattca tttcttttaa
    15481 tgcatttcgt aaacaaataa tcatttcttc atttattttg taaaacacat gtcctttgaa
    15541 tacatcgacg tcacctaatg ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt
    15601 cccaacactt aaacaagctt gaattaaaga atacaataaa taggttttcc caccaccgat
    15661 tgcaccagca atcaacatat ttggtgattg atcatatacc caatccaaag ttttcattaa
    15721 atgaatacgt ccattttctg ctatacattg atcaatagga atccgaccag aatctgaaag
    15781 gaatacataa ttgattcggg ctttttctcg ttcttcacga attaaatcac gcaaaaatac
    15841 ttcagataac aaatgaccca tttttaaaaa tcgttcgtga aaacgcccac catctaaagg
    15901 aaaagaaacc actaattctt gtttcttttg ataaaaataa acttttggta atctcatttt
    15961 ttcccttgtt tgtttctcac taacttgttt ctttgtataa acaaaccgat tgtcaattaa
    16021 cattcgaaca agtaactgac gccaataaac agaggcaaaa tatccttctt ttagtcgcga
    16081 ccaatgcatc aaccctaaaa ttaacccact aaccagcaac aaatacaaca caatacttat
    16141 tccaggaata agatacattt gccaattcga aaacgtactt aaacgtggat aaaatacttt
    16201 ccaactaaac caacctaaca gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa
    16261 ccataaataa cgattgaaat agcgaattcg ccgaccacga tacttgaaaa aatgagcttt
    16321 caattgtcac attcctcctt tctgttttta cttttcctgt gctaaaaact tttgacaaaa
    16381 agcaactaat acaggaatat cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg
    16441 atttttataa gggtgtgcac acatattaca tattcgtact atacgaatcc aaatcacttc
    16501 ctgatgatct tgtttccact ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat
    16561 acactgccca ataaccgcac aaatatatgc atttgtttgt acaaaaatcg ttggattgtc
    16621 tccaaaatac tcaatcaatt gttggatatt ttgataacaa gcacaatctc tcaaagtatt
    16681 tgattggttt tatctgaatt caaaatctcc ccttccttct atcttagata aaaagaatag
    16741 gaagactcct tcctattctt tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa
    16801 cacttggttg tttttgatct aaaccttttt cttgtgattg tttcatatta ggattatttg
    16861 ttggagcttc tactttctct aatcctttcg cataaatcac taaatctgaa tattcacgat
    16921 tgccatcttg tgctggaata tataccacat gatattctgg atcaattaat ttcacaggat
    16981 cacgtcgttt aaaaccagtc aaatctacat ctgctggaaa tttaactcgt cgtgcaatct
    17041 ttggttcatc tggatttcca aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat
    17101 aatcgcctgt ttcattatca aatacacgat cagactcttc taatgcgcca aagcgtacgg
    17161 ctcctaaaca agtttcatct aatgcttctg taatgtcata attatttgga aattttaatc
    17221 ccatcttaca aaacctcccc aatttttaca ccagttaaaa agacaccaac atcttgatag
    17281 ccagttgttc gacgctctgt ttgtcccata tcatttgttt cacgactatt cactcgttct
    17341 ttacgaaaaa caaaataagc ttcttcaaat gaaatacgca cctctttttc tggtaatcgt
    17401 aatcttttat cctcttcagc tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa
    17461 cactctgctt gtaattgaac atatcgccca gacacaccat taatttcctt tggtggatta
    17521 ggttcccgtg aacccaataa aaatacttga cctaaaaact ctttcttcat acgcaatgct
    17581 ttttctgcca taaagttttt cctcctctta aaaaccaaaa taaattctaa atatttctac
    17641 attacaatca aacaagcggt taaaaatcct aaccgcttgt tcctcatgac taatctttgg
    17701 ctttacgtac tttataaagt gttataccta aagcaatgac taatactact aatccaagaa
    17761 ccataagtat actacttgtt tgttcacctg tttttggcaa cattggtaca acagtagctg
    17821 gtttaacccg ttgttctggt ttcactggtt gcggtggttt tggcgcttcc acaatttgaa
    17881 ccgtttgatc tttgtttgtt agatctacat tgtgtttcgc cacttcttca aacgtttctt
    17941 tctcatcttc cgttttcgct tggagccatt ccgcaaaact caagcctttt cctgcaagat
    18001 tgcgaccgtc cactaagaat tcaaataaga attccatcat ttctttatcg gccgcaaaga
    18061 ctttttccgc atgagtgcct tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa
    18121 ttaagcgata gatttgacca atcgcaagac cgtctacttt gacccattca agcacagttt
    18181 gatttggcaa ggccaagatc tcttttccgc catttttatt cgtcgctttc gtcgtaatgg
    18241 ttggattact gattttgatg gtttggtcat tgtcggtaat atctgcgtga gaagccaata
    18301 atacgccttc tctaaacaga tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg
    18361 cgtctagaat aaagtctaga ttgactgtac catccgttgt ttctgccaca aacgttaagg
    18421 tacttgtgac ttcttttcca tctactagca ctggtttttc cgttactttg tccattagaa
    18481 caccgttgac tgtgtatgtt tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa
    18541 tggtgacttt cgttaatgga ttaacctctt tcaagccagt gggtttatgt gtagccgttg
    18601 tacgtacact tgggttagtt actcgtaccg tttgcccttt gtcgttgatg tctttatggt
    18661 ttgctagttc ttcatcattt ctaaaaagac tttcaaacac gacgatttca cgaccattta
    18721 tacctgtcaa atcaaaggta aatggtacga tcaccgtttc attttctgct ttggcaataa
    18781 agccaacact cgtttctacc gtatttccat gcacaagcaa gggttcaccg gttgctttat
    18841 ccattaagat ccctttgacc aagtatgctt ttcctggaag aatctcggta atttcaacgg
    18901 tatcgtttaa ggtaaacaat cctgttgggt ctgcgactgt caatccgtct tcaaagacag
    18961 ccgtggtatg aacggttgga ttattcactt tcacactttg ggcttcatcg ttgatatccg
    19021 catgtgctgc aacttcaatg ccatcacgga aaagtttttc aaaaacaacg atcgtttgac
    19081 ctttcagaac actagcatta accacaaatt caaggtcttt tgtgccattg actttgtctg
    19141 caataaaggc caaggtactt gtgatttctt tgccatcttg taaaattggc gcattcgttg
    19201 ccttgttcat caatactcct gaaatggtgt attcttttcc gacaaacaag ttggtataag
    19261 aaacactgtc tttaatgacg atcgtttcta atggatcaac cactttttca ccatcggctt
    19321 tatttgacgc ttgtgtttct agttttggtg gaatcaagta attacgtaca gaaaccacta
    19381 acgtttcccc atcatctggc gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg
    19441 tatttggttg gaaactttct aatggggcat tttccaagaa gtaatagtca tcggcaacta
    19501 gacctttcac taaaatttcc ccgttttgat cagtggtata ttcaccaatt gcttgatttt
    19561 taccgttata caaggtaaag ttcacacctg cgagcggcgt tttcttcttg ttccattctt
    19621 catacttgaa taattttaca cttttccgtg cttgacggtt cgcaaaatgg atcactttga
    19681 tcggttctgt atcattggta atcgagaaca ctaatgggtt ttgattcaaa acgtaattta
    19741 cgggggcttt gatttcttcc aaacgatagc gatcttttcc ataagtaagt gtaccgttag
    19801 ttaggaaaaa gcctttttca ttggttttaa agatatccgt tgtttctgta tcatttggaa
    19861 tctccattgt gacataacga ttggctaaag tatcaaaaat cttgaattct gtattagcaa
    19921 gtggaatcac ttttcctgtt tcttgatcca ctttcaccac tttaagacgt tgttcaatta
    19981 ctttatcttc taaaacataa tggaaagttt gcccattttc agaaattgtg acttcaagtg
    20041 gttcaaatag tttataccct tcttttcctt tggtttcaga aagaatgtac gtatcaaaga
    20101 caacggtatt tttaccaaaa gtagcataac catcattgtc tgtcacaacg gttttcacca
    20161 ctttgccagt tgtttttgac gtcaaagtga tttcaatgcc agctaatttt ggtttgttat
    20221 ctggattcgt tgaatcgact aaatctttat tagcaacttt cactaaatca atggttcctt
    20281 tgatcacttg atcttttgcg gtcgcattga cagtttttcc aggttcaaca acaatttcaa
    20341 ttgcttcatc agaaagcaca taaccttttg gcgctttgat ttcttttaaa acatatttgc
    20401 caggatcaag atctttaatt tctgcctgta ctttgccgtt aatttttttc aaggtaattt
    20461 ctttaatctt tttcccatct gcttgataca aaccatagac cgcattttct aaagaagctg
    20521 caccttgtgc tttcacacca gtttccgcat cttctttaaa cacacgaatg tttccttgtt
    20581 ttagaatttg taaattaata acagacgcat taggttctgt tggatgtaac acaccaattg
    20641 tttgagctcc tggacgacga taggctaatg gcgcaccttg aactttaatt ttattcaatt
    20701 gaatttttgc tttatccggt gcctcttttg tgactgtaat ccatacttca ttgccgtgaa
    20761 tagatacatt gacacctttt ggtttttgaa tgtctttcac atatttcaaa aaatcatttt
    20821 tattctttac tttcaacgtt tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg
    20881 ctttttcacc aaagttatta atcaaacgtt gaatttctgc ttttcgacga tcataatcaa
    20941 cacggatatt tttgattggg atttttgtgc ccaaagcttc ccaaatcata aattgtgccg
    21001 caatgtattg ttcatcggat ttgtctttat ttgtgacata tcctaaataa gagatcaagc
    21061 ttgttttttt ccgtacattt gaatttttca aatagttttt caaagcagta gctgtataaa
    21121 cttgaccttc tgcggctgag acaccaggct caatacaaaa cgccacttgt ccattaatct
    21181 caaaacgaac catataattt aaagtgaagt ttagattgtt cggactagaa gtaacaccaa
    21241 tattttcgcc ccaatcaatt ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa
    21301 cacgaaccga acgattggtt ggtactgtta ctaacagtgt aggtgtacct gtttttggaa
    21361 tgttctgctc ttttttcttt tgattttcaa tactagccgt agattctttg tttggtttcg
    21421 ttggttgatt ttgactagct gcggttgaag agattgtttt ttcaacgttt tcctcatttt
    21481 ttccttcttt gtcagcagta gccgctggtt gttccactgt ttcttctatt cgtttctttt
    21541 cttccgcttg tttctcagaa gactctggct gcacagaagg cgttgcttcg gcagcttttt
    21601 ctccttctag agtggacgtt tctgaagcag gggttgtaat tgaggcagtt tcttcaactg
    21661 gttgtgaagt accaggacta ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag
    21721 tcgttttgga attgaccgtt ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac
    21781 cagctgggac cccggcttct gctggttgga cttctgtcac ttcagaagcg acttgggcaa
    21841 tggcttccat cgtaggttgt atagccgtcg tttcttctgc atgtgcttga gcagttccca
    21901 ccaagaaaca gaattgagaa aaagccacca atgcgagtgc tttcaaccaa ttcattttcg
    21961 tttttgcata cacaagaatt cctccttttc ctgagaattt tatataataa aacaaaacgc
    22021 cgttaccaaa agtgataacg acgtgaactg tctcttctat ttttatctgg cgctcttatc
    22081 tgaacacatt tcatcaatga ttttaccctc ccactataat tacagaacaa aaaaagaacc
    22141 tgcttcgtgg aaatcaactg tccatttccc aattggaata ccagcttttt tggcaacttc
    22201 accatcacca aatctcatgc cataccaatt actaccttca tccttaattt ctttccaacc
    22261 atatgacata gcttcatcta atgaatcaaa aatcatgaca ctattgccaa ttgttacagg
    22321 atctggctta agatcttctg gaagagaaat atcaatcata ccataatgat ccgattcctc
    22381 agtattattg tcattactag ctactggttt ggttgttcta ctggcttatt ttgatacaac
    22441 aagtactctt attcttcttt tacttctccg ttttcaattt cccattcaaa ccactccgca
    22501 aagtcttgct taaaaccatc tactcgatac ctattttgta aataggcatc ttctgacgtc
    22561 gtaggatcta aataggtagg tttattctct ggacgagcat agacacctac ccatgtttcg
    22621 taatctcctt caaaataact atctaccaaa tagccttttt ttctaagaat ctcccgaact
    22681 tgatttttaa ttattgatac ttgttcaaaa ccatcactca ttctatcacc tcaaataact
    22741 ctagattctc ataaatattc ccgacaattt caatcctatc aatgatctct aacaaatcgc
    22801 tacttatgct tccccattcg tataagaatt tcccttccgc aaaataaatt ttccataaac
    22861 ttcttcatga tcgtcccaac caatatcact ctcgaaaaat tccacgttgt tcttatcttt
    22921 tagctcagta aatgccataa gttcaacatg ttttccaacc tcgaaacaat cacaccaccc
    22981 atcaaccgtt acacgtgtgc cttcaatacc taagaaactt tgtcctcgtg tattaaacac
    23041 taaaactttc cgcatttttt gctcattaat taatcacgcg cgaaatctag gaatcacaac
    23101 ttattcgctt cttctttata aataaaaaaa ctccaataat cggaaattac tatcttttcc
    23161 tcattccttt tttaaatctt catacttctt cttatctttg tcctaaccta ataacacaaa
    23221 ttttgaagaa aaactcagta aaacctgatt ctttttttat cttcacaaca accaactttt
    23281 tcttcagatc cttttactag aacttgtata caaaaattgt tgaatgctga tataatttgc
    23341 ggctacttga aaaaacgata gatgtaaaaa gataaatag
//
LOCUS       DAZUMI010000036        23358 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000036 DAZUMI010000000
VERSION     DAZUMI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.036"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..995
                     /locus_tag="ACG81C_001218"
     CDS             138..995
                     /locus_tag="ACG81C_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5064391.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="ACG81C_001219"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="ACG81C_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5064392.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="ACG81C_001220"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="ACG81C_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5064393.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="ACG81C_001221"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="ACG81C_001221"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5064394.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /gene="pstB"
                     /locus_tag="ACG81C_001222"
     CDS             3751..4509
                     /gene="pstB"
                     /locus_tag="ACG81C_001222"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5064395.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="ACG81C_001223"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="ACG81C_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5064396.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="ACG81C_001224"
     CDS             5437..5736
                     /locus_tag="ACG81C_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064397.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5777..6469
                     /locus_tag="ACG81C_001225"
     CDS             5777..6469
                     /locus_tag="ACG81C_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5064398.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACG81C_001226"
     CDS             6466..7233
                     /locus_tag="ACG81C_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5064399.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACG81C_001227"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACG81C_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5064400.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACG81C_001228"
     CDS             9198..9518
                     /locus_tag="ACG81C_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5064401.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACG81C_001229"
     CDS             9518..9868
                     /locus_tag="ACG81C_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5064402.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACG81C_001230"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACG81C_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5064403.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACG81C_001231"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACG81C_001231"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5064404.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACG81C_001232"
     CDS             11862..12887
                     /locus_tag="ACG81C_001232"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5064405.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACG81C_001233"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACG81C_001233"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5064406.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACG81C_001234"
     CDS             13965..14405
                     /locus_tag="ACG81C_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5064407.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACG81C_001235"
     CDS             14408..15010
                     /locus_tag="ACG81C_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5064408.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACG81C_001236"
     CDS             complement(15078..16184)
                     /locus_tag="ACG81C_001236"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5064409.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG81C_001237"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG81C_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5064410.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="ACG81C_001238"
     CDS             complement(17731..20076)
                     /locus_tag="ACG81C_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5064411.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="ACG81C_001239"
     CDS             20355..21257
                     /locus_tag="ACG81C_001239"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5064412.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="ACG81C_001240"
     CDS             21470..23245
                     /locus_tag="ACG81C_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5064413.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7443 a         4142 c         4926 g         6847 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggacta
//
LOCUS       DAZUMI010000037        23009 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000037 DAZUMI010000000
VERSION     DAZUMI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.037"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACG81C_001241"
     CDS             135..2606
                     /locus_tag="ACG81C_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5064414.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACG81C_001242"
     CDS             complement(2709..4445)
                     /locus_tag="ACG81C_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5064415.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACG81C_001243"
     CDS             complement(4442..5146)
                     /locus_tag="ACG81C_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5064416.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACG81C_001244"
     CDS             5277..5780
                     /locus_tag="ACG81C_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS5064417.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACG81C_001245"
     CDS             5740..6078
                     /locus_tag="ACG81C_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5064418.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACG81C_001246"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACG81C_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5064419.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACG81C_001247"
     CDS             7629..8207
                     /locus_tag="ACG81C_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5064420.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACG81C_001248"
     CDS             8211..8732
                     /locus_tag="ACG81C_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5064421.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACG81C_001249"
     CDS             complement(8811..9365)
                     /locus_tag="ACG81C_001249"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5064422.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG81C_001250"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG81C_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5064423.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACG81C_001251"
     CDS             9905..10156
                     /locus_tag="ACG81C_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5064424.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACG81C_001252"
     CDS             10281..11072
                     /locus_tag="ACG81C_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064425.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACG81C_001253"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACG81C_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5064426.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACG81C_001254"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACG81C_001254"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5064427.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACG81C_001255"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACG81C_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5064428.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13370..14032
                     /locus_tag="ACG81C_001256"
     CDS             13370..14032
                     /locus_tag="ACG81C_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064429.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14111..17338
                     /gene="fss3"
                     /locus_tag="ACG81C_001257"
     CDS             14111..17338
                     /gene="fss3"
                     /locus_tag="ACG81C_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5064430.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17452..17766
                     /locus_tag="ACG81C_001258"
     CDS             17452..17766
                     /locus_tag="ACG81C_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5064431.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17779..18153
                     /locus_tag="ACG81C_001259"
     CDS             17779..18153
                     /locus_tag="ACG81C_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5064432.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18163..18507
                     /locus_tag="ACG81C_001260"
     CDS             18163..18507
                     /locus_tag="ACG81C_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5064433.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18578..19930
                     /locus_tag="ACG81C_001261"
     CDS             18578..19930
                     /locus_tag="ACG81C_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5064434.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            19990..21132
                     /locus_tag="ACG81C_001262"
     CDS             19990..21132
                     /locus_tag="ACG81C_001262"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS5064435.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21157..21411
                     /locus_tag="ACG81C_001263"
     CDS             21157..21411
                     /locus_tag="ACG81C_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064436.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21667..22851
                     /gene="mobT"
                     /locus_tag="ACG81C_001264"
     CDS             21667..22851
                     /gene="mobT"
                     /locus_tag="ACG81C_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5064437.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22848..22985
                     /locus_tag="ACG81C_001265"
     CDS             22848..22985
                     /locus_tag="ACG81C_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5064438.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7644 a         4091 c         4955 g         6319 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatcag aagataaaca cggaaaactt ccttggaaat
    13021 ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt cttttttctc tatcacagtt
    13081 ttaagccatt tttggctgtt ttggaccatt tttcaatcat attcccttaa aaattgtcct
    13141 agattgtccg acattgacta tcagtgtcgt caaaataggg caaaatttag tagtcagctt
    13201 gacgacaaat ataatacgaa aaataccaaa acatttaccg aatttagcaa cttttgaacc
    13261 caagtttctc aaagggtcag ctattagcaa aaaagacaaa acatgtactt ttctactaaa
    13321 aagttgatgt gataggcttc tatctaaata actattagga gtttttatcg tgaagcaaaa
    13381 tgatggacgt atcatttgga aaaatcaaag tgagttaaaa ctgattttaa caattaatga
    13441 attcatcgaa aaacacggaa ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa
    13501 tccaaattct gcaccaagta tgtggttcat taacaaaaaa tatggatcat gggagaattt
    13561 attgattagt ataggacgag aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca
    13621 agaattattg gaaatagtag aagcctttat taagtgcgag aagataacct ctcaacgaat
    13681 gtacgaacaa aaatcagtag ggaagaatat tccttcactt agtaccatta aaaagatgtt
    13741 aggagatata cgtccacttt ttaaagaaaa aaatgatgga tctcgattta ctgatttcga
    13801 actgctgtta gaactaaaaa atgaaataat ccgattaaaa ctacaggatg atttatcaat
    13861 gacaaagttt cgaaaactag ttcaatcccc aaaactacca tcagttgata caattatgaa
    13921 aagaacgaac aaaaattggg aggaattgat ggcagagatt ggatttgatt atcgaagaat
    13981 caaaatttat aagcagagaa acaacctatc caagacaaag aaaactaaat agtatcactc
    14041 taaatcagac gttcaaaatt aaaatttgga cgtctttttt attacccaaa aacgaaagga
    14101 gatttatttt atgaaaaagc caagatttaa gaattggcgt ttgctcgcaa cactgagtct
    14161 attatgccaa acaattggtg gatcgcttgg cccaacgatt gcctttgctg atgaaattac
    14221 tcatccacaa acggtaactg tggaattgga tttggctcac cagtatgctg tcgaaggtac
    14281 atttagcgat ggtcgtccca tgtcagaagt taccgttccg cactatgcag tttacaatgg
    14341 tgtgaagcaa gatattttct gtattgagcc aggggtgccg atttacaatg agttcactcc
    14401 tggttatgag aaaaatccat tgcctgatat gtcggagaaa gcgaaattag tttccgtttt
    14461 atggaaggaa gctggtacgg atgtggatac acatattgtg gcccagaaga tgatttggca
    14521 agaagttaat ggctacacgc ttcactcaat taaacgttct gatggtagtg cagtaaatat
    14581 cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat tatcaaaaga aaccaagctt
    14641 ccataactcc acagtgaaaa ccgtgttagg tcaatcgact actgtgacag atacgaacgg
    14701 cttgaattta tcggagtttg atgaagttgt cgagaatatc gcaaatattg attatcgtgt
    14761 gaacggcaat caactagtga ttactccaaa tgccaattca aaagagagtg gtgaattaac
    14821 cctaaagaag tcagctaata caggaacacc ggtagcttat aaaatggctg gtcaacaaac
    14881 tttgatggcg ggggcaattg ataagcccaa tacctacaca atcaaaattg atgtggaaac
    14941 tgagggttct ttgaagatta aaaaagtcga taaagaaaca ggaaatgttg taccgggaac
    15001 cgttttccat ttagactttg gaaaaacatt acctgcaaaa gacgtgacca ccgataaaga
    15061 aggcattgcg acattggatg agattcctca tggcgcgaaa gtaaccatta ctgaaaaatc
    15121 agtgccagct ccttatacga ttgacactac accaatcacc gcaaccatta aatctggtga
    15181 gactatttct gtgacttcaa aaaatactcg cgaaaaaggt caaatcattt tgaataaaat
    15241 gggggtggaa acaggaactg atctttggaa cgacaaatac tcattagccg gaaatacgtt
    15301 tgccattcgc aaagacagtc caaccggtga aattgtccaa gaaatgacca cggatgaaaa
    15361 aggtcatgcg gaaacaccaa aagagattgc caatgcgttg gaattgggaa cctattatgt
    15421 gaccgaaacc aaagctagta atggctttgt gaataccttt aaaccagtaa aagtcgaatt
    15481 gaactatgtc aatcaaaccg tggctcttgt taccagtaac gtaaaagggc aaaaccaaga
    15541 aatcactggt gaaaccactt tgacaaaaga agacaaagat accggtaatg aaactcaagg
    15601 taaagctgag tttaaaggag ctgaatatac tctctttact gcaaaagatg gtcaagcggt
    15661 caaatggagt gaggctttta aaccagagtt agtgaaggga acgaaagctt ctgatgaaac
    15721 ggtgactttg actttagatg aaaagaatca agttgctgtt aaacacttag cgattaatga
    15781 gtattactgg caagaaacca aagcacctga aggatatacc ttggatgaaa caaagtaccc
    15841 tgtttccatc aaaaaggtgg atgataacga aaaaaatgcc gtgattaccc gagatgttac
    15901 ggcaaaagaa caagtcattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
    15961 tgggactgcc gaaactggct tcaatgatct gacctttaaa gtatcgccat tggaaggaac
    16021 cagtgagatt acaggtgccg aagatgaagc aattacagct tataacgagc aactaggttt
    16081 tgatggctat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
    16141 ggctccagaa ggatttcaaa aaattacgcc attagaaatc cgttctactt ttaaagaaaa
    16201 taaagcggat tacactaaga gtgaatacat ttttacgatt accgaaaaag gacaaaaaca
    16261 accaattaaa acggtgaccg ttccttacga gaaactgacg aataaagcat tttctgttag
    16321 cttgaatcgt ttgatgctct atgatttacc tgaagaagaa gatagcttaa cttctcttgg
    16381 gacttggaaa gacggaggca aagaattgac agaccttgat tctaccgagc tagttgataa
    16441 attgagctat aacttgcatg aaatcaaaga agactggtat gtggtagcgc aagccattga
    16501 tgtggaagcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtaattgc
    16561 cgaaacaacc gcaacgttgg cgaataaaga gaaaacagga acttggaaaa ttttgcataa
    16621 attaaccgca gaacaagttt tggataaaac catcgtctta ttcaactatg tgtatgaaaa
    16681 caagaaaccc tatgaagcag gtgatgatcc agtagcgaaa gatgctagct tgaacaatca
    16741 agcccaaact gtcaattgta cggtggaacg ccatgtttcc atccaaacaa aagcccacct
    16801 agaagatggt tcgcaaacct ttactcatgg ggatgtggtg gatatgttcg atgatgtgtc
    16861 gattacccat gatgtactgg atggctcaaa agaagctttt gaaacaattc tgtatgcatt
    16921 acttccggat aatacgacca aagaaatttg gaaatctggc aaaattgatt atgaagtgaa
    16981 tgacaaagaa ttcactaaaa ccgtacttgc ggaaaaagtg gacacaagta aatatccaga
    17041 aggtacaagt tttaccttca aagaaattaa ctatggcaaa gacggaaaca tcaatgggaa
    17101 acacaatgag gatctaaaag aaaaaactca aactttaaca cctaaaaaag ttccgacaac
    17161 accaagtgca ccagaacaac cggaaatacc aactgttcca agtgactctc aagaatctag
    17221 tcctacagta aagacattcc cacaaacagg ggagaaaaat tctaatgttc tgctgtttat
    17281 tgggtttacc ttgatctttg cgactgctgg gtattatttc tggaatcgcc gaaactaagg
    17341 tgatgtgatg aaacaactaa ataggaaaaa ccaacgtgcg cgaccgcctc ctaaaaaaag
    17401 tggtcgcgct atttttgtat ccaaaactaa aaatgaaaag aggaaaagaa gatggaattg
    17461 aaatttgttg tgccagatat ggccgaaacc tttggaaaga taaggtatgc cggcgaaggc
    17521 gaagttttaa ctgaaggata tgggcggaat actacagtaa ttggtcgtag ttatcatttg
    17581 tattccagta agcaacgagc cgatgatatt gaagtggtgg tagctgcgga agccggcgaa
    17641 aaggattttg accaagatca accactaaaa gccgtgaatc cccatttggt tgccaaaggc
    17701 tatgagattg agaatcgtgg gtttaccgac tacgtgttgt atgtcgatga tttagtgaaa
    17761 gcataggagg aaaagaagat gagattaaca gaaggtattg ttgtagattc aggactaacg
    17821 tttgggaaat tacgattttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga
    17881 acggttagta acgaggtaaa agaacggact tataatttga aatcctctgc gcaaggtcga
    17941 atgatccaag ttagtatccc agcaaatgta cccttacgcg agtttgccta tgacgcagag
    18001 gtagagttgg tcaatccaat catggatacg gtcgccaatt atgttttccg agaaggaact
    18061 actgtcaatt ggttcattaa ggccgatgat ttagtcttga aacgacaacc agatcaggga
    18121 aattctacaa accaaaatga aggaaagaaa taggtgaagc gtatggaaat taatgttgaa
    18181 catattttag attgtctcga tcaatatgga aaaggtgagc taacggagga acaactcact
    18241 aaagcattaa cctatgatga aaaaatgttt ctgattatgc atcaagggtt actggaagta
    18301 ggtaacgatg tcgaagaaga ttttgatgta ctgaattggt taggggaaga agagtccttc
    18361 tttatggtta tcgaagtaaa tgaaagtcta tgtcgagaag ccgaaaccgt attggaagaa
    18421 attggtgtgg aaatgccgga tgccattgaa gtttttttaa agcaactagt ggaaacgaaa
    18481 caactcccta tagcagtaaa tgactagcat aactgcttga aattggatca atgaaatcct
    18541 atgaagcttt tttcgattgg aggtggaaac cataaaaatg tacaaagggc atcgtatacg
    18601 ggcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat
    18661 cggttggatg agcgtctttt attttcaaga gcttagacaa tttgatattt ctaagctatc
    18721 actttctact atagaaatcg ttcgttctat aaaagattta atctatttgc taggaagtct
    18781 cgttctttca ggaagcacga tgttgcttta tatacacttt tttcaggatc attggcgcag
    18841 tttgtggcat cgacaaaagc tagcccggat gattctagaa aatcattggt acgaagtaaa
    18901 acaaactcag agtgaaggat ttttcaaaga cctcaatagt agtcgaacca aagagacaat
    18961 cagttacttc cccaaaatct attaccgtat gaaggatggt ttactttcta ttcgcgttca
    19021 aatttcactg ggaaaatatc aagatcagct tttaaagctg gaaaagaagt tagaaagtgg
    19081 tctatattgt gaactagtag aaaaggaact caaagactcc tatgtggagt acactttact
    19141 gtatgatata attgccaatc gaattgggat agacgaagta gtggcagaaa gtggggcttt
    19201 gcgattgatg aaaaatcaag tatgggctta tgattcttta cctcatatgt taatcgctgg
    19261 cggcacaggt ggtgggaaga cttacttttt actcactatc atcgaggctt tattaaaatc
    19321 tgatgccgag ctttttgttc ttgatcccaa aaatgcggac ttagccgatt taggtacggt
    19381 aatgcctcat gtctattccc aaaaggagga aatttctgct tgtgtggaag atttctatga
    19441 gcgcatgatg actcgtagca aggcaatgaa agaaatgccc aactacaaaa caggagagaa
    19501 ttatgcgttt cttggacttc caccaaactt tttaatcttt gatgaatacg tggtttatat
    19561 ggaaatgtta acgacgaagg aaagtgcggt gattttgaat aagctcaaac aaatcgtgat
    19621 gttaggtcgt caatctggtt tctttctgat tctagcttgt caaagaccgg atgcgaaata
    19681 tttaggagac gggattcgcg atcaatttaa tttccgggtg gctttgggtc gtatgagtga
    19741 actgggatat tccatgatgt ttggcgaggt tgataaaaaa ttctttatga agcgtatcaa
    19801 aggtcgaggc tatgtagata cgggagggag tgtgattagt gagttttata cgccacttgt
    19861 accaaaagga tatgatttct taagggaaat tagcgacatt ggtggattaa acgttaatac
    19921 ggaaggataa aataatagta tgtagcctag gagtgtcaag aatcctgggc tttttttacg
    19981 aggtgattta tgacattttt agatttattc gctgggattg gtggcttttg cttaggtatg
    20041 gaacaagcgg gtcaccagtg tatcggcttt tgtgaaattg atgattttgc tcgccagagt
    20101 tataaggcaa tccatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    20161 gacgagttta ttcaatctct cggaccagtg gatatccttt gcggtggatt tccgtgccaa
    20221 gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt
    20281 gaaatcgctc ggttcgccgc tattctccaa cctaagtttt tattccttga gaacgtccgg
    20341 ggattactca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    20401 ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaaaccgt
    20461 gagcggatct tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    20521 attggatcgt ctccaacatc tgatcaaaac atcagaaact tattaaatat caacccttcc
    20581 aatcgaggaa tggggggaca agtctatggg tcagatgacg tagcgcctac cttgaccggt
    20641 gatgaaggaa tcaaaatcgc attgccggta gatgatggaa tttctgtagc gggtatgtta
    20701 ctgggaaatt ttgaacaagg caatcgggtt tatgagataa ctggaacagc gcctactttg
    20761 tcaacgaaac aaggcggaac gaagatcatg cttcggaaaa acgaaacaga atatcaagag
    20821 gtgaagccag ggaacagtgt gaacctggct tttcctaatt ccacaagtcg tcgaggccgt
    20881 ctgggaaaac aaagtgtgca taccttatta acaggagacc aacaagcagt cgtcacggat
    20941 cagtatcaaa ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccggac
    21001 tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa gcaggctgga
    21061 aattctgtaa cagttccggt catttttgat attgctagaa gattaaagga ggtaaaaaca
    21121 gatgatttat gatgagttga ttggggaatc tactgggtga ttggcaagat tcagtcagat
    21181 ccggaattgg aagaagaact gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa
    21241 gtccctgatg atccgtatgt aattgatgag gaaacggatg ctcgaatgga gctgaatcaa
    21301 gtcatcagtg aattggaacg aattgcggac tttgctaaag aacctgatat ccgccaatac
    21361 ttgtttgaga taaaagcaga gttggaaatt gagggtatta ccgcggagta acggccaaat
    21421 aaaccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg
    21481 tgcctgttta tggacggcgt tagccggcca tgatgtctca ccccccgtat ctaacagggg
    21541 ggtacaaaaa cacaaaaaat aagcaagcaa cgataaggaa tggctgtcct atcaaggata
    21601 ataagcaatt tgtgtagatg ttaactgatt tgaaaaagtt aacaccttaa atttggaggt
    21661 gagaacttgg cacaaaggaa tttagattat cggctattaa aagaccggcg taatgaatat
    21721 ggcgtttcac aaaataaatt agctacgact tgtggactta gtcgcccgta cttaaatcag
    21781 atagaaaatg gtggcgtaac cgcatcgaca aaaaccatga ggaaaatttt tgaccaactg
    21841 gaaagtttca atcccgagtt acctttaacg ttactgtttg actatgtaag gattcgcttt
    21901 cccacaacgg atgcgaggaa aatcattcaa gagattctcc atttgaaatt tgattatatg
    21961 ctccacgaag attacgcctt ttactcctat caagaacaat atgttatggg agatattgtg
    22021 gtgatgttgt cacatgaaga agataaaggc gtccttttag aattaaaggg tcgtggttgt
    22081 cggcagtttg aaactttttt actcgcccaa aagcgtagct ggtatgactt tttcgaagat
    22141 tgtctaaaag ccggtggcgt gatgaaacgc ttagacctgg caatcaatga tcgagttggc
    22201 ctgttagata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
    22261 cgtaccttca aaagctatcg ttccggggaa ctgttgaaac ctgatgataa agatggtatg
    22321 ggcaatactt tgtatattgg cagtcttaag agtgaggtct atttctgttt gtacgaaaaa
    22381 gactatgagc aatatatcaa gttagggatt cctttagacc agaccgaaac gaaaaatcgt
    22441 tttgaaatcc gactgaaaaa tgatcgagct tatcatgcaa ttcaagattt gttaaaaggt
    22501 cgcagtattg aaagtaccac attttccatt attaaccggt acttgcgatt tgcggataag
    22561 gtggaaggca aaaggcgaac taactggccc ttgaatgaac aatgggggcg cttcattgga
    22621 cggaatcaga aggaaattca attaacttct gaaccgaagc cttataccat tgaacgaacg
    22681 ttgaactggc ttggacgaca agtcgcccct acgtggaaga tggccaagga gttggatcgg
    22741 ttgaatcaga caacgtacat tcaggatatg gtaaataata ctcgactatc agaccggcac
    22801 aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa
    22861 tcgtgatgga aattgtgaaa gatattttat tggtgtttgg tggcagtatg cttggtgtta
    22921 ctgtgatgtg cttgatgcat gcaagtgcag cggcggaccg agcaatggaa aagaaaggaa
    22981 agtgatgaaa tgaatttcgg tcaaaattt
//
LOCUS       DAZUMI010000038        22801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000038 DAZUMI010000000
VERSION     DAZUMI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.038"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG81C_001266"
     CDS             3..371
                     /locus_tag="ACG81C_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5064439.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG81C_001267"
     CDS             551..1492
                     /locus_tag="ACG81C_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5064440.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG81C_001268"
     CDS             1561..2292
                     /locus_tag="ACG81C_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5064441.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG81C_001269"
     CDS             2436..4616
                     /locus_tag="ACG81C_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5064442.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG81C_001270"
     CDS             4636..5118
                     /locus_tag="ACG81C_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5064443.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG81C_001271"
     CDS             5134..5895
                     /locus_tag="ACG81C_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5064444.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG81C_001272"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG81C_001272"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5064445.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG81C_001273"
     CDS             7667..8152
                     /locus_tag="ACG81C_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5064446.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG81C_001274"
     CDS             8145..8828
                     /locus_tag="ACG81C_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5064447.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG81C_001275"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG81C_001275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5064448.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG81C_001276"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG81C_001276"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5064449.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG81C_001277"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG81C_001277"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5064450.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG81C_001278"
     CDS             13125..13457
                     /locus_tag="ACG81C_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5064451.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG81C_001279"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG81C_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5064452.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG81C_001280"
     CDS             14283..15656
                     /locus_tag="ACG81C_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5064453.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG81C_001281"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG81C_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5064454.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG81C_001282"
     CDS             16711..17151
                     /locus_tag="ACG81C_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS5064455.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG81C_001283"
     CDS             17295..17996
                     /locus_tag="ACG81C_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5064456.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG81C_001284"
     CDS             17993..19114
                     /locus_tag="ACG81C_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5064457.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG81C_001285"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG81C_001285"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5064458.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG81C_001286"
     CDS             20493..21401
                     /locus_tag="ACG81C_001286"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5064459.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG81C_001287"
     CDS             21436..21708
                     /locus_tag="ACG81C_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5064460.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG81C_001288"
     CDS             21719..22765
                     /locus_tag="ACG81C_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5064461.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7685 a         3979 c         5006 g         6131 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 a
//
LOCUS       DAZUMI010000039        22215 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000039 DAZUMI010000000
VERSION     DAZUMI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.039"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..648)
                     /locus_tag="ACG81C_001289"
     CDS             complement(130..648)
                     /locus_tag="ACG81C_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5064462.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(650..1267)
                     /locus_tag="ACG81C_001290"
     CDS             complement(650..1267)
                     /locus_tag="ACG81C_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5064463.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1264..2118)
                     /locus_tag="ACG81C_001291"
     CDS             complement(1264..2118)
                     /locus_tag="ACG81C_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5064464.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2115..3026)
                     /locus_tag="ACG81C_001292"
     CDS             complement(2115..3026)
                     /locus_tag="ACG81C_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5064465.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3067..4401)
                     /locus_tag="ACG81C_001293"
     CDS             complement(3067..4401)
                     /locus_tag="ACG81C_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5064466.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4609..5439
                     /locus_tag="ACG81C_001294"
     CDS             4609..5439
                     /locus_tag="ACG81C_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS5064467.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5683..5932)
                     /locus_tag="ACG81C_001295"
                     /pseudo
     CDS             complement(5683..5932)
                     /locus_tag="ACG81C_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6123..6317)
                     /locus_tag="ACG81C_001296"
     CDS             complement(6123..6317)
                     /locus_tag="ACG81C_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064468.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6822..7007)
                     /locus_tag="ACG81C_001297"
     CDS             complement(6822..7007)
                     /locus_tag="ACG81C_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064469.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7857..8198)
                     /locus_tag="ACG81C_001298"
     CDS             complement(7857..8198)
                     /locus_tag="ACG81C_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5064470.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8198..8428)
                     /locus_tag="ACG81C_001299"
     CDS             complement(8198..8428)
                     /locus_tag="ACG81C_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5064471.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8715..9053
                     /locus_tag="ACG81C_001300"
     CDS             8715..9053
                     /locus_tag="ACG81C_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064472.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9258..9820)
                     /locus_tag="ACG81C_001301"
                     /pseudo
     CDS             complement(9258..9820)
                     /locus_tag="ACG81C_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10552..11133)
                     /locus_tag="ACG81C_001302"
     CDS             complement(10552..11133)
                     /locus_tag="ACG81C_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5064473.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11316..11942)
                     /locus_tag="ACG81C_001303"
     CDS             complement(11316..11942)
                     /locus_tag="ACG81C_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5064474.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11964..13295)
                     /locus_tag="ACG81C_001304"
     CDS             complement(11964..13295)
                     /locus_tag="ACG81C_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5064475.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13450..13851)
                     /locus_tag="ACG81C_001305"
     CDS             complement(13450..13851)
                     /locus_tag="ACG81C_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5064476.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13937..14143)
                     /locus_tag="ACG81C_001306"
     CDS             complement(13937..14143)
                     /locus_tag="ACG81C_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064477.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14238..15740)
                     /locus_tag="ACG81C_001307"
     CDS             complement(14238..15740)
                     /locus_tag="ACG81C_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5064478.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15758..16213)
                     /gene="argR"
                     /locus_tag="ACG81C_001308"
     CDS             complement(15758..16213)
                     /gene="argR"
                     /locus_tag="ACG81C_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5064479.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16311..16928)
                     /locus_tag="ACG81C_001309"
     CDS             complement(16311..16928)
                     /locus_tag="ACG81C_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5064480.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17149..18522
                     /locus_tag="ACG81C_001310"
     CDS             17149..18522
                     /locus_tag="ACG81C_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5064481.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18597..19709
                     /locus_tag="ACG81C_001311"
     CDS             18597..19709
                     /locus_tag="ACG81C_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5064482.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19781..21418)
                     /locus_tag="ACG81C_001312"
     CDS             complement(19781..21418)
                     /locus_tag="ACG81C_001312"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5064483.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21586..22194)
                     /locus_tag="ACG81C_001313"
     CDS             complement(21586..22194)
                     /locus_tag="ACG81C_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5064484.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6480 a         4531 c         3871 g         7333 t
ORIGIN      
        1 ttataacgtt ctcatttaac aaaacttaag ttattaagac aaatgaataa acaactacgc
       61 tttcctcgaa acaaaaaaat aaagcacaaa tgcgcattta cgcactcatg ctttattcgt
      121 acaattaatt cagataacgg cagcgcatag gatgccaata acacttccta aaatagctcc
      181 tcctaaaatg tcagtaggat aatgtaaaag taaaaaaatc ctcgaataag cgatcaaaca
      241 agcaaataaa aaggctaata ttcctgcaac tggacttaat tcgaacaacg cagctactga
      301 taaaaaggct agcgctgtat gtcctgaagg aaaagaagaa gtcttcggtt tacttgtgta
      361 aaggacaagc cgacgatttt tttcataagg tcttttccgt cttgtcgttt ttttgataaa
      421 aagcaaacca acaaaaaaat agatcaaact gccgataaga atggctagac catattctct
      481 agtatcaggt atcaagacta aaacagcagc aaggagcaac caaataaaac caccattaga
      541 gatattactg ataaatttca aaaaattgat ttttcgacta tccgttttcc gttgcttggc
      601 gaacttgttg ataatactat catcgatttt agcaatcaaa cgcaacatgt tattcctcct
      661 ttttaagaac cttttcttgc gtacgatgga taaaaagcag gacttgaaaa taaaacaaga
      721 aactgaaacc taataaagca aaatagattg gtcctgcaaa ccagaatgcg acaagaagga
      781 tcaacgtgcc tgccaaatag aaaagcgcgc ttttgaaatc attaaaaaac aagaaaaatg
      841 cagtaatcca agaagcttct ttttgttcca gttgaagcaa gattttgtaa ctaaagagaa
      901 gcgcgcttaa tagaagcaaa aacaacgtag tatacatcaa tgtatagcca attcccccac
      961 cccaatagcg gaaaatctga taatcgatca atagaagaag aaaataaaca cttgctaaac
     1021 tatcttgctt tccataaaga gagaagttct cacgaaactt tttccagaat agtgaaccga
     1081 gctttttctc ttcatttttt ttgtaatgat aaatcatata cagaactgtt cgacttgcag
     1141 ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa tggataagac
     1201 gtggaatcca ttcaatgatt tctaagaaat aatcattggc ttttttacca atcgatcgat
     1261 tcatcatcct tttgaacctc ctgtcaaatc catcccttca atgaaataac gttgtgcaaa
     1321 aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct cctgccatca gataattcca
     1381 ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc tgtaacgtgt atttttcttg
     1441 atcttggata tataacagag gacctaagaa atcattccaa gctgcgttga aggaattgat
     1501 cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc aaatatacaa
     1561 atgattcgct ccgtcgattt cagcagcttc gatcaattca ttattgatcg ataagtaaaa
     1621 ttgacgcatc agaaatatat tgaacgcatt gccgaagaac gatggaacga tcaggggcaa
     1681 ataagtacct acccagccga tcttggagaa taatacatat tgagggatca tcgttacaaa
     1741 ccccgggatc atcatcgtag aaagaaccaa agcaaacatc agctttttcc caggaaaatc
     1801 aagcttcgca aatccataag caataaaaga atttgaaagg acattaccga tcactactag
     1861 cacagtgatg aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg caatcgtatc
     1921 cagatagttt tcccatacga ttttttctgg gaaaaaggtc ggaggataac gaacgatttc
     1981 ttccattggt ttcaatgaag taaacaccat ccaaagcaat ggaatgatca aaatgattcc
     2041 tattactgtc atgaggacat aacttagtat tttttttgtt tgccgattca cactggtctt
     2101 tttgaaataa tccatcattt gccgtcacct ccagaataat agacccactt aggtgcaagc
     2161 ttcaaattga caaacgtcag aaccaacacg atcagaaaca ggatcattga catagccatc
     2221 gcataaccca tatcaaaagc tacgaacgcc ttgttccaca tatgcagatt ataaaaaagc
     2281 aaagaatttg caggtccgcc tgttccattt ttcgtcatga cataagcttc ttgaaaaatt
     2341 tggaaagaac cgatcgtaga ggtgatcaca tcgtaaaaaa taataggagt gattaatggc
     2401 aaagtaatac ttttgaactt tccccaaacg cctgctccat cgatttctgc tgattcatat
     2461 aaagatctag gaacattttg taatgtagct aagtaaagta gcattgctcc tccagaactc
     2521 catattttca tcaaaatcaa cgcaggtttc gtccattgtg gatcaaacag ccaagctggt
     2581 ccagggatat ggaaccagcc cagaaatgtg ttgatcaatc ctgcagatgg agaaagcagc
     2641 tgcatccata agatgtagac tgctactcct gataagactg ccggtaaata gaaagctgta
     2701 cggaatacac tgattccttt gattttttga ttgagtactg tggctaaaaa cacactgaaa
     2761 atcgtcgtca gcggtacatt gaagatgacg aaataaacgg tgttgtataa cgatttccaa
     2821 aataaatcat cgtgaacgaa cattcttttg aaatttgcta gaccaatgaa attcatcttg
     2881 gatgtcaaat tataatcggt gaaactacca ataactgaaa aaagcatagg ccctagcata
     2941 aataccaaga acccgataat aaaaggcgag ataaataaat aggcccatct tgcttctttt
     3001 ttctttcgga tagataaacg cttcaaattt tcttgcctcc tctgtaccta gacagtcaaa
     3061 aatggattat tgattttgct tgactaagtt ttctactgct ttttgtgcat ctgccaatcc
     3121 ttcttttggt gattttgttc ctaaaagaat ctggtcaatc tgttcattca ctaatgtcgt
     3181 gtaatccgga gcatctaaag gaactggtgt gatcaccgta tttttaaagt tttcgtccgc
     3241 catttgatag atcatttttc ctttatccgt taggttgtca ttattcacta aattctcatt
     3301 ggctgtcttg ctcgccatga tatcaaaact tttttcgccg aatttttctt gtacttctgg
     3361 agaagaaaga tatttgatga attcataaga ggcctcagga tcttttgctc cgtatggtac
     3421 ttccaagacg aatccgccac cccacgacca atggcctgaa ccttcttctt tttctgggag
     3481 ctgcatcacg ccaaaatcaa aatcatccgg tgcattttcc atcaaactag agtaataatt
     3541 gatattttgg gcacgcatgg ctactaatcc ggaaataaat ggatcagata caccagaccc
     3601 aaattcggct tctaatttat tgatcgtatt tttcccataa taatcttgcc agtcttggat
     3661 ccactccaag gcttccactt tatttttagt atcaatcttg actttttcat ctttgtcaaa
     3721 ccaactagtc ccgttatctg cgtttagcgc ccatacatca gcccctaaat tccataatgg
     3781 ataaaaaccg atgcgtgtaa attcttttcc ttctttttta tctagtttac gtgctgtttc
     3841 ttctaattca tcccaagttg tcggtagatc ttgttcactg attccagctt ctttaaataa
     3901 ctttttatta taaaaaatga cttgtgtatc tgtattgaat gggatcgcat aaggatcacc
     3961 ttcatattct actgtatccc aaagctgcgg ataaaaatcc ttagaagtgt ctttttctac
     4021 atatttggag agattagttg cttgctgtgc ttctgctctt tgggcaacgg aatttatgtc
     4081 ctgtacaata acatcgggtg catttccagc tgttattgca gataaagatt tagtccagat
     4141 gtcaccccat ggctgatact tataatttac atggatctcg ttttgtgatt gattgaaatc
     4201 ctcaatgatt tcttcgatag cttcttttcg tgcaccggat ccccaaaagg accagaaatc
     4261 aatatttttt cttccatcac ttgttttttc agaagaattt gctgcacttg aactggcatc
     4321 gcttgcatta cttgatccgc atcctcctaa taaaaacaat gctgaacttg ccagcaaccc
     4381 tgtaaacaat aattttttca tattttacct ccaaatttta atgtaaccga tgccggtttt
     4441 aatataacga tatcattttg ttctgaaaat aacaatttat aaggtatgat tctaaaaaaa
     4501 ttattaatga taccaatact ttatataatt cgcgagcgct ttctttgaat tttttaatct
     4561 ttcgtagcag aatgagaatg tattaaaaat agattaggag gaattttgat gtttagacac
     4621 cagaaagaat tgcagtttga agcaaaacca gatcgtccga atccattaat cgcgaaatat
     4681 ctgcaggagc tgatcggcgg ccaatatggc gagatgagtg tagctatgca gtacctgttc
     4741 caaggctgga gctgccgagg agaagaaaaa tataaagata tgctgatgga cattgcaaca
     4801 gaagaaatcg gccacgtgga aatgttggcg acaatggttg ctcgtttatt agagggtgcg
     4861 cctgttgaag atcaagaagc agcgatgcaa aaagatccaa caatcggtgc cattatctct
     4921 ggaatgaatc cacagcacgc gatcgtagct ggcttaggac ctcgtcctgc tgacagcgtg
     4981 ggcaatccat ggagcggtgc ctatatcatt gcgagtggca acttgttagc agactttaga
     5041 gctaatttaa atgccgaatc acaaggaaga cttcaagtag cacgtattta cgaaatgatc
     5101 gatgatcgtg gcgtaaaaga tatgctgtct gttctccttg caagagatag ctatcatcaa
     5161 aatctatggg aatgtgcagt gaaagaatta gaagaaaaag aaggttcaat cttagtccca
     5221 agtactttcc cgcgtgacaa agaacgaaaa gacattgctt atgacttcta taacttctca
     5281 gagggagacc aaagtagtga aggttcttgg gctagtggca aagcactgga tggcgaaggc
     5341 gaatatcaat ggttgaaaga acctaagatc gaaggcgctg caccaaaatt agaaccagta
     5401 acaccaaaaa tgtatgggac acctccattc aagaaataaa tatctacccg tagaaacacc
     5461 cgttttttgt tccacacgct ttgtgagcaa aactgactaa agtcattaat cataaccacc
     5521 cttcaaaagg gtggttttct ctagagctat aagctcaaat caccggccag cgcctaaaga
     5581 cgctggcttt catattttat gttcaagcca tattgccttt gccagttttg cccacctctt
     5641 aaaaggttta tgtactactc cttagccctt aaaaggatcg tcttactctc gtacactcag
     5701 cttatctaac gcactatcat gtttttcttg ttcttgaatg tactttttga tggtcgcttc
     5761 attttgtcct acttttgtca catagtatcc ttccacccaa aagtgtcgat acccaaattt
     5821 atattttaga tttgcacaat ctcttgatga tctcctgaat attggctcga tattgattat
     5881 aaattatttt tcttctatac tttggtgtga atacaatgtg gtatttacac atctactttg
     5941 tacgggctaa actatttccc tttattgctc cacccgcata gcaagtggtt ttgtatttca
     6001 atcgctcggc gcgctcaatt aggtctaaag acaataaatc taaaaacccg ctgcttcact
     6061 gacagaaaca acgggttttc agaaaaaaat gaaatgaaaa agatctctta cggcaatcta
     6121 ttttattttt ttgatttttt ataaatggaa aatgcttcaa gaagtccaag aaataaagcc
     6181 gtataaaaac aagaacctat gaaatcttta tttattagat attcaacaga aatcccgata
     6241 acactagcca acaatatatt tatcagaagt gtaaaataag gatgcttttt atagaataga
     6301 actagtgttg ttttcataga ctctcctttc ttcatatttc caattaatca caaattcacg
     6361 gcattactag aaaaattaaa ggggcgcaga agtggaaaaa tactcttcga ctttccctac
     6421 aaaccaggcc atcttgtgtt taataactat accaaatcta aggtacctct aaatctaaat
     6481 tccatttgtg gcggtgacct ccaattttta actcttctat ctcccatcca gtatacataa
     6541 ccttgatact tcgaccactt atatatcgaa cacacaaata cctatccatt gcaggagtac
     6601 tacctaactt agttgctact tgcatctaaa taacagctga aaagatagga cttactctta
     6661 gtaaagtatc tgaactatta atttttaaac tatcattttt atcctcaact acatgcacat
     6721 ctgttgcaac ttcactttct gtctcatcca gataaatgat taaggaatcg ggaattgcaa
     6781 tatcttcagc tgacgctgga atagttgagc taaattagtc atcatatctt tattataaaa
     6841 taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc attaataaat
     6901 gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa catataccct
     6961 ataaaagcta ataagatggt taggaatcct ttagaatttt tactcattca tagacacttc
     7021 cttaaataac ttttattata gaaggtattg taaataataa tattgacaaa aagtgactca
     7081 aaataaaaaa aaccttacat tatactggaa tgaactgtat tgatagtgta cctaaaagct
     7141 ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg ggatgtaatc
     7201 aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc gctttgagat
     7261 tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa gtattggtgt
     7321 cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc taatttctgt
     7381 atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg catgatatgc
     7441 ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct tgatcccaaa
     7501 ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct aaagagttta
     7561 gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa agtattgtcg
     7621 cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac acaaaaaaac
     7681 gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt ctttagttta
     7741 agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt acttggcctt
     7801 tttttaattt cgttatgttt agcatataaa aatattatag agtttactta caatgcttaa
     7861 aacatatact gtataatctg atcaatttta gccatgtctt gcaatggtaa gttttctatt
     7921 tttttgagtt ttcgttcctt aaaatcaaga gattttaatt gtgtaataat tacttgtcct
     7981 tttatatcta gctcttcagg taatgagtaa cgtgtcggta aattctttat agtggaagta
     8041 attggacata tgaccgaaaa cattgtttta cggttaaatt cataacgaga gacgactagt
     8101 cctggtcgtc ttttttgaat ttcttttcct acggagggat caaaatcaat ccatacaata
     8161 tcaccttttt taggaatata aattctttct tctgacacta taaaatttct ctcccttcag
     8221 gagttaaatc ttcccattcg tctttctcat aatactcggc ctcttcccca ccactaaatg
     8281 gatcttcaat ttttggaact aacgtaattg ttccatcatc agaatacaca acgatatatt
     8341 cttggttttc tgaaggtttc tttccgttat cagaaggtaa agttaccact acagaacttc
     8401 cttgtaatct agattttgta acaagcatta tcatcactcc cttaataaaa gtatactata
     8461 taacactaag taacgcaaaa ttcccctttg taaactatct agaaaattgt tttgtttaaa
     8521 gtatatctta ttggatataa atagaaataa attgtagcca gttcattttt tctttaacta
     8581 aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa tactatagct
     8641 aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa
     8701 caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca
     8761 aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca
     8821 aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg
     8881 ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa
     8941 ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg
     9001 ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa
     9061 catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata
     9121 cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt
     9181 ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct
     9241 ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg
     9301 atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca
     9361 agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt
     9421 gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt
     9481 tccttatttg aaaacataaa tttgtctttt tcttcattcc actcattggg aaaacacaac
     9541 gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct gcacgggcat
     9601 taatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt tccggagtga
     9661 ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa acttctccag
     9721 ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa taattctgaa
     9781 ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct cccaatcttt
     9841 ttttctatca tacactgcaa caagaagttc gcaccaacaa aacgctttca atttttgtag
     9901 aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa tggtattctc ctgccaactc
     9961 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gctttttgaa attgttcctt
    10021 gtgactcgga gtgaaacggc aggcttacga cctctgggta aacggtgttc aaaagccagt
    10081 ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ctattttcac aaaatttttc
    10141 ttaatactcg aaactaagcg actttttcac aacctcttcc ttaaacggtg ttcaaaagct
    10201 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gcagctattt ttatttggct
    10261 gttcttatta cttgatgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat
    10321 cagctaacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
    10381 gtttctctaa tactcaggtc aaagcgctga tttcacaacc tctaatacac ggtgttccag
    10441 aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
    10501 aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt attaaagaac
    10561 cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa gtagtgatag
    10621 atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca acgctttggc
    10681 ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga tattttgttt
    10741 cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt cttcatccac
    10801 tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga tctgtgtctc
    10861 atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct gttttccagc
    10921 atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat ggatcaccgc
    10981 tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc ctgtatcaga
    11041 agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa taagatcttt
    11101 ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc atttctcata
    11161 gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa aaagactgtg
    11221 ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag atgttcattg
    11281 attcaagaac tgcgaggaca aaagcaattc ctatcttatg atagttgctc taatacttcc
    11341 tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc atccattgct
    11401 tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc actctctacc
    11461 aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag aattactgtg
    11521 tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc aaagatttta
    11581 tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc gataaaatcg
    11641 gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat ctgctgctca
    11701 agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg gtaaaaataa
    11761 tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat ttcaatcata
    11821 ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc accgcctgct
    11881 ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt attattgaac
    11941 atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc actcattgtc
    12001 ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt catagctagc
    12061 gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc tagaatcaag
    12121 tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa tacttgtgct
    12181 ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg tgctttggct
    12241 tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact gtaaggcaca
    12301 gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat aatgattcga
    12361 taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc aatcgcccaa
    12421 atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg atctgttagt
    12481 ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc agttgcagcc
    12541 aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt gacggcagct
    12601 gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc gattcccgca
    12661 tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg aagaaattca
    12721 gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga tgcaaattcc
    12781 aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc ctgatccatc
    12841 gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg taaaatgatt
    12901 ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt ttcaccatta
    12961 ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat cactgtccca
    13021 gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt caatttttct
    13081 ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat caatgattta
    13141 gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga cttttcaacg
    13201 actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc gccctttcca
    13261 aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct tttttaccta
    13321 ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca tctgctttat
    13381 ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg ttcgatttcc
    13441 tttattcttc taaacaatcg ttacttctac atcgatattt cctctagttg ctttggagta
    13501 agggcagaat tcatgcgcat cttctgcaat tttttgggct tcttcttcgc tgatgccatc
    13561 gatatgcact tctaatacta caccgatctg atagccgcca tcatctaaat tataaagaga
    13621 gacacgtgct tttactgtgg actctgctgt cattcctgct tctttgatca tatgttgtac
    13681 cgccccattg aaacaagaag cataacctgc tgcaaataat tgttctggat ttgttccttc
    13741 tgcatggatt cctggaggag tgattttcat atgcatcgag ccatttggtg cctcgacttc
    13801 tccattgcgc ccgccatggt tgatcattgc tgtttcgtat gcttttttca ttgtactgct
    13861 cctttcgatt gaccatttgc tacttggcta taattatagt gtatgacaag taaggcgctt
    13921 tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc ttctactatt tttccaaaaa
    13981 acaaaaaaat actactcgtg aacaacagcg tcatatttgt tccaaaataa atagcaatct
    14041 tttctacata atcttttata tgattgattt taagatacat atataccaga aagttgaccc
    14101 cataaaccaa tactgttaaa aaaataacca tattcgttac cattttttcc tccttttgct
    14161 ggtctcccta ccagcactcg atttaaccat accacttttt ttgccgtcca agcaactagc
    14221 ctttcaaaaa gactggttta ttctgctgtt aatgattcaa gatcagaaaa atgtccgaat
    14281 aattttgcta attttactaa cgccaacttg ttctttccga attcatcatt ttctatctgt
    14341 aatactagta acggctcatg taagcttaaa cggatcaaaa accagccgtt tccgtatggc
    14401 tcttctagat catatcgaat accttcttca ttttccgggt cgaagttcat ccctttttct
    14461 tcttccaaca gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg tggttcatcg
    14521 ctgaacaatt ttaatcggat ttcttgtact tctacgggct gtttcaactc tttgatttgt
    14581 tcatctaatg tgataccttt tctttttaat tctggcaaaa gcatcaggat ctttgcgatg
    14641 acatatgcac catcatccag aaaatagttt tccttgaatg cagcgtgtcc gcttgtttcg
    14701 atggctagct gtgtatcttt cccaagacgg ttaagttcta atgctttatt gattacattt
    14761 cgatagccgg aaatataacg aacttggatg cctccttttt cttcaatgaa tctttgtaga
    14821 tgagaagaag taggggaatt cgtcacgatt cttgttcctg gatgctcttt taatacgatt
    14881 gtacttaata ctgcgatcaa attatttcga ttgatcactg ttccagaagc actgacaact
    14941 gcagaacgat ccacatctgt atcgaaaatg atcccaagat ctgcttcatt tgctaaaaca
    15001 gcctgcttga tactttccat cgcttcttta ttatcaggat ttgggatatg attaggaaat
    15061 cggccatctg gttccaaaaa ttgggaacct gttgaatccg cacctaagct attcaatact
    15121 ttttcagcaa agaatccacc agcaccattt ccggcatcaa caatgatttt ccaaccattt
    15181 agtggtttgc tttctagtgt tccaatcccc tttttaattt ttgtgactaa atcttgcgca
    15241 taaggtgtca aaaggtctgc tttttctacg cttccagttt gttcgctttt tattttttgc
    15301 tgctgtgata ggataaaagc aatatcttct ttttctgctc ctccttctga ggtaaagaat
    15361 tttattccat tataataata gggaagatgg ctagcagtca gcataaccgc agcatcgcac
    15421 gagaactgtt caaattgagt ggacataaac atagcaggcg tagtagctaa ttcgaaatcg
    15481 atgacatgta tcccttgttc tttcagactt tggatcaacg cctctttcag tgctggtccg
    15541 cttatcctac tgtcatgacc aatcccaatc gtccattttt tatttggatc ggttgatttc
    15601 ttctttttct ttatccagtt tgctagtccc cagccgatct tttttaccat gtctgtcgtc
    15661 aagtttactt catatccttc aactggtaat gcgattcctc gaatatccga tccattttgc
    15721 agcgctttca tatctttcat ataaaagtcc tcctcattta ttccctcatt ttttccagct
    15781 tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat aataatgaat gtatctacac
    15841 cggccatcgt tccgacaact tcagggtaag ccacttcatc tagccaattc gttaccacat
    15901 cggcaccatt tcgttcagtc aatacgataa ccatgaactg aacagactgg atacgcaaaa
    15961 cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa agaaacagat ggctggttaa
    16021 ataatgtata gcgtactatt ttgttttcat tttgactttt tactaacttc atttctcgaa
    16081 tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc cgcgcaagca
    16141 gctcttcctg tgtcccaata ggttgttctg taatgatctg gcggatcagt gcttgtcgct
    16201 gtttcttttt catattagac acctctcaat gttgattata gcaaaagata agtaatattt
    16261 cttcatattt tggtattttt tacttttatt ttttgttttc attcaggttg ctaacctttt
    16321 tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct tggatcgctg
    16381 atgtaaatct ctttgtgttc tttagaagct ctttcatagc cttcttttgc gatgaatgct
    16441 tctaatttct caaacgttgc tggttcttcg gcatagggcc cgacgtgtaa gatttgggca
    16501 caaagacctt cttccatctt tttgaatact acttgttctt tcaagtcttc cggaagtttg
    16561 ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg ctgtcggatc
    16621 atcaattgat aagaaaaatg ttcttttttc atcactggtt catgcaaata cttttcttgc
    16681 agtccccatt gtccttccag tggatagaca gtatacatct gataatcagg aatcagccaa
    16741 ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc tactcttcgt
    16801 ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga ttttttagga
    16861 acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg ccattcaatt
    16921 ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat acctcttttt
    16981 gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat aagtgaagta
    17041 aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt ctatttttat
    17101 aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat gacaaaacaa
    17161 aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt tgcgggcgta
    17221 ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt tgatttaacg
    17281 actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat cattgttccg
    17341 ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc ttcaaatctg
    17401 cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt cctattgttt
    17461 ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt ccatattatc
    17521 ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac tttgacaacg
    17581 gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca ttttacatgg
    17641 aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt ctttgattat cggctggttt
    17701 gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag cgttttagga
    17761 atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac agtttccagc
    17821 tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg aagtaaaaaa
    17881 gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg cttgtttttg
    17941 attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt gccgaatttc
    18001 cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct tcccggcgca
    18061 gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata tggtccgaag
    18121 aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt attcctactc
    18181 ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat gatcggtatc
    18241 ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga agaactacaa
    18301 ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt ggctacttca
    18361 ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta tgaagtgtct
    18421 actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct cgtaagcttt
    18481 gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata tacggcaggt
    18541 gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc ttttttatgg
    18601 aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt tacttagata
    18661 catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact aaagctagcg
    18721 ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat cgtctggcta
    18781 aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat caaacgattt
    18841 acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa ttggctgttc
    18901 ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg ctttcacctg
    18961 aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt atcagtggtg
    19021 gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta gcacaaaaac
    19081 aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct tggctcatca
    19141 agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc ccggcactta
    19201 agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa gggcagctct
    19261 tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg cttgacggag
    19321 aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc catgaaaacg
    19381 atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact aagagtacag
    19441 aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc cgtgtaggta
    19501 cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat gatccaagct
    19561 tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat atcggctatc
    19621 tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa aaggacatct
    19681 ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa ggatcccgag
    19741 cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat ttctgaatca
    19801 aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc tgagcttcaa
    19861 tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac gtatcctcaa
    19921 aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga ccaaacgact
    19981 tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt tcaggtttga
    20041 tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc tgtttcaaaa
    20101 acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa ggattagcaa
    20161 atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc gcgtctgatc
    20221 gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg atcttgataa
    20281 ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt tcgctaagat
    20341 gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt ccgtttcgta
    20401 tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc acattctgac
    20461 aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt ttatcaaagt
    20521 aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa gcatcgtcat
    20581 gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt gattttccag
    20641 aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca tattgtttca
    20701 tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca agcgtcaaag
    20761 tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta ttatggagag
    20821 catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga tcacaaaaga
    20881 tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag atcttcgaat
    20941 tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct tctggaatca
    21001 gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt gtatggtaaa
    21061 actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat ttgctttgtt
    21121 ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca atgcttccat
    21181 cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca tgtgagattg
    21241 ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct gtcttcctat
    21301 caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat cgaatactgg
    21361 ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt gtactcatgg
    21421 ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt cgagttttta
    21481 tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa aaccgagaac
    21541 ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt accagccgtt
    21601 tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta catattgttc
    21661 tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag catctaattg
    21721 ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt ctttttgtgc
    21781 gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg cagcaggagc
    21841 tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg tttcagttgt
    21901 agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat ttgaagttgg
    21961 gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat ttttttcagc
    22021 aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag ataatgaatc
    22081 acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta caaacattgc
    22141 agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat tcatattaaa
    22201 ctcctttttt tctcc
//
LOCUS       DAZUMI010000040        22134 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000040 DAZUMI010000000
VERSION     DAZUMI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.040"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..523
                     /locus_tag="ACG81C_001314"
     CDS             <1..523
                     /locus_tag="ACG81C_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5064485.1"
                     /translation="GGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVA
                     NCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGS
                     LIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVL
                     LLPLFFQPFAGLF"
     gene            523..2109
                     /locus_tag="ACG81C_001315"
     CDS             523..2109
                     /locus_tag="ACG81C_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5064486.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2128..2349
                     /locus_tag="ACG81C_001316"
     CDS             2128..2349
                     /locus_tag="ACG81C_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5064487.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2346..3107
                     /locus_tag="ACG81C_001317"
     CDS             2346..3107
                     /locus_tag="ACG81C_001317"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5064488.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3487..3996
                     /locus_tag="ACG81C_001318"
     CDS             3487..3996
                     /locus_tag="ACG81C_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5064489.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4071..4610)
                     /locus_tag="ACG81C_001319"
     CDS             complement(4071..4610)
                     /locus_tag="ACG81C_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5064490.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="ACG81C_001320"
     CDS             complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="ACG81C_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5064491.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5623..6474
                     /locus_tag="ACG81C_001321"
     CDS             5623..6474
                     /locus_tag="ACG81C_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5064492.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            6502..7137
                     /locus_tag="ACG81C_001322"
     CDS             6502..7137
                     /locus_tag="ACG81C_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5064493.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7157..7930
                     /locus_tag="ACG81C_001323"
     CDS             7157..7930
                     /locus_tag="ACG81C_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5064494.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8338..9135
                     /locus_tag="ACG81C_001324"
     CDS             8338..9135
                     /locus_tag="ACG81C_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5064495.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9113..9526
                     /locus_tag="ACG81C_001325"
     CDS             9113..9526
                     /locus_tag="ACG81C_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5064496.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9510..12155
                     /gene="mutS"
                     /locus_tag="ACG81C_001326"
     CDS             9510..12155
                     /gene="mutS"
                     /locus_tag="ACG81C_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5064497.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12173..14281
                     /gene="mutL"
                     /locus_tag="ACG81C_001327"
     CDS             12173..14281
                     /gene="mutL"
                     /locus_tag="ACG81C_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5064498.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14303..14863
                     /locus_tag="ACG81C_001328"
     CDS             14303..14863
                     /locus_tag="ACG81C_001328"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5064499.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14948..16936)
                     /gene="treP"
                     /locus_tag="ACG81C_001329"
     CDS             complement(14948..16936)
                     /gene="treP"
                     /locus_tag="ACG81C_001329"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5064500.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17159..17872
                     /gene="treR"
                     /locus_tag="ACG81C_001330"
     CDS             17159..17872
                     /gene="treR"
                     /locus_tag="ACG81C_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5064501.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17949..18395
                     /gene="msrB"
                     /locus_tag="ACG81C_001331"
     CDS             17949..18395
                     /gene="msrB"
                     /locus_tag="ACG81C_001331"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5064502.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18566..19168
                     /gene="ruvA"
                     /locus_tag="ACG81C_001332"
     CDS             18566..19168
                     /gene="ruvA"
                     /locus_tag="ACG81C_001332"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5064503.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19181..20182
                     /gene="ruvB"
                     /locus_tag="ACG81C_001333"
     CDS             19181..20182
                     /gene="ruvB"
                     /locus_tag="ACG81C_001333"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5064504.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20211..20789
                     /locus_tag="ACG81C_001334"
     CDS             20211..20789
                     /locus_tag="ACG81C_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5064505.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20841..21323
                     /locus_tag="ACG81C_001335"
     CDS             20841..21323
                     /locus_tag="ACG81C_001335"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5064506.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21440..21814
                     /locus_tag="ACG81C_001336"
     CDS             21440..21814
                     /locus_tag="ACG81C_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064507.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(21927..>22134)
                     /locus_tag="ACG81C_001337"
     CDS             complement(21927..>22134)
                     /locus_tag="ACG81C_001337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064508.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7237 a         3991 c         4790 g         6116 t
ORIGIN      
        1 cgggggcaat tttttgacta tgctgattac tcctgcgaca gtggcacttg ccatccctct
       61 ttataaaaac ttccgtttac tgaaagagaa tttttatcct gtaatggctg cgattttagc
      121 aggaatagtc gcaaattgct tggttagtgt agggttcggt tacttcttct ctcttcataa
      181 agagatggtg atctcattac tacctaaatc tgtaacgacg gctatttctg tcgatttatc
      241 tcatacgatg ggcggggtga atgccgtgac acttgctatt gttgtctcta caggtatttt
      301 tggttcgctg atcgccactc atattttccg cctattcaag atcgaaagtc cagttgctcg
      361 cggtgtggcg ttaggaagta cttctcatgc tattggtaca gcaaaagcaa tcgagattgg
      421 tgaaatagaa ggaatcattt ccggacttgc gatatgcgtc aacggaatat tgaccgtttt
      481 attgctaccg ttattctttc agccttttgc tggcttgttt tgatgcgaaa accactattt
      541 tatgctacaa taaccaaaac gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct
      601 tccgagtatc ttaaacaatt tgataaagat ctatattata agattctcct cctagaaagt
      661 tttgaggatc aagcttggca taccgctgca caattagcgc aagttgttca gctggatgcc
      721 cgcagcgtgt cgaaatacct gaacgagctg tctaaaaact atcagcagtt tagcggtaag
      781 acacaccctt tgtttacaaa aaatcaccgt tctggttata acttttatga tacgctcgat
      841 tcaattgaac atgagcgttt tttgatctat ctcgtgcaaa gtacgctgaa attccagttg
      901 ctgcacgaca ttttttttga agaattccat acaatgtacc aatttgcgca aaagcattac
      961 attagtgaat ctactgctca tcgaaaaatc aatgaatgga aacagcaact gcaaacttac
     1021 ggaattcggc tgcaacgagg tacatatatc gctcaaggcg aagaagaaat cattcgactt
     1081 tacttgcata tgacattctg gcagttgttt cgagggaaaa tctggccatt tgaaacgatt
     1141 tctcaaatgg acgtaaagaa tatggcagaa catatcatgg ctttttttaa tgttcgtctg
     1201 aacgagatca aaaaaagacg attggaatat atgctaggtg cctttttttt aagaaaatca
     1261 cagaagcatt atgttgtatt gaatgagaaa aaaagacggc tgatttctga caatctgttg
     1321 ttccagcgat tttgccaggt catggagccg gtatttccta attattttca agtagaagac
     1381 gagttaggcg cattatttct tgtactgatg actcgtgaag aatattatag tgatcctaag
     1441 atacggaaga aaatttttga ttttcatcaa caagcaaaaa caccgccctt tacagccctt
     1501 tcagaagcaa aagccgctct ttctctttat caggaagagc aggggctgcc agcagaaaat
     1561 ctaaccttcg aggcagaaaa ctatttattc agcagtcatt ttttcgccta tctttttcct
     1621 aatgcgaaag aaacgattga tggcaatagt agtgatttta ttaatcattt agttattgaa
     1681 aacaaggaat tgaaacaatg gctcgtacat ttttttgaaa gtagacacaa acatcccaat
     1741 catctggcat ttaaaaatca tgcgtttttg atggggcggt atctgactgt gtttaaaaca
     1801 ttaggagcct ttactccaca gcttccaaag atcacgatcc ttttaatgac agatttccca
     1861 ttatttgaag agcaactgtt agaggaggga ttacggacat ttttccgtaa tgagtatcag
     1921 ctgatttttc tgccaacaga ttatcggggg agagaagttg atcttctgat ttcgacaagt
     1981 aaagtccacc gcaaaccatg ggcggatctt gactatttta ttgtgactga agaactaaaa
     2041 ttaattgatt acatccaact atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta
     2101 aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc agaattgaaa
     2161 aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt tgttctcgta
     2221 tccgatgatg aatttgatgg ctgtccggtg acgccgtact tattgatcaa aacaagtgac
     2281 gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga ttgggcgatc
     2341 gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc acaggagctg
     2401 cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct tttgtatttg
     2461 gattagatca aaagttcggt aaaatggaag aaatttcttt gcgatttcct gaaacttttg
     2521 cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta tcgaaatgcc
     2581 aaaatcattt tggtgaagtt acgattttac tgaatacagc ggggatattg gatgcctaca
     2641 aacccttgat ggaaactgac gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa
     2701 gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccaaaaa agcggaacga
     2761 tcatcaatat ggcttcgatt gctggcttga ttgctggtgg aggtgggatc gcttatacaa
     2821 gcgcgaaaca tgcgattgtc ggatttacca aacaactcgc tctggattac gcaggacagg
     2881 gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat gcggctgatt
     2941 ttgaagggaa aggtgagatg gcagagtggg tggcaaatga aacaccagtc aagcgatggg
     3001 cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa gcaagctaca
     3061 tacaaggaac gatcatccca atcgacggcg gatggttgtt aaaataacct tgtgtttttt
     3121 tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa aaaaagtcga
     3181 tgactgttcc cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt
     3241 taatattcga aactaagcgg cttttcacga cctcttgata taaacgatat tcaaaagctt
     3301 gcaactgcgg taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacca
     3361 ttcttattac ttgttgcagg gcagcttttt cactaccttc tgcgacactt cagttggaat
     3421 tttcttccca tcctttggtc gcggtcatta ccaagataaa attatcttgc aaaggagaaa
     3481 tagaacatgg aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact
     3541 cggatggcgc tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc
     3601 tttggagtga ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa
     3661 agatacatcg tcgcggtaac agcgggagta gctttggcta atattgcttc tcctcttgga
     3721 ctgatagatg ttgcagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa
     3781 atcactagtc gcatcaaaaa catgaaaatc aaaatggccg tgacagcatg cgtatttgct
     3841 ttttccatgt ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg
     3901 aattggtggg tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt
     3961 tattggatcg gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaataggg
     4021 cagcatggaa gagactgaat gttgagtctt cttccatgct gcttttttga ttaatttgta
     4081 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     4141 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     4201 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     4261 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     4321 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     4381 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     4441 ccaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     4501 gtttgccctg tcaaaggaac caaaccaaaa ggaatggtca tctgagaaag gacagaaata
     4561 atcgcggcaa attccgcagc aagtatcaat tctttcaatg ttcggttcat aacgccctct
     4621 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     4681 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     4741 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     4801 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     4861 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     4921 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     4981 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     5041 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     5101 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     5161 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     5221 gtattctgat tgtttgccac ggctattagg tccatgttga cctggtgcgt aagggcgacg
     5281 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     5341 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     5401 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     5461 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     5521 ctgcattatt ttacacaaat tacagtatat gatagatcat ccacgaatgt caagataatt
     5581 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gtatgtgcaa
     5641 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     5701 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     5761 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     5821 gcgatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     5881 atgttcagct ggggtctggt catgattcta tacatgaacg ccgaattagg aacatcgaat
     5941 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     6001 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttctaatt
     6061 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     6121 gtaaaactag caaaaccttc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     6181 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca
     6241 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     6301 cctgccttta cgcttactta ctttgcttct cacggacaga tggatggaat gacagtcagc
     6361 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     6421 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     6481 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     6541 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     6601 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     6661 ttagcttttg ttcattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     6721 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     6781 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     6841 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     6901 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     6961 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     7021 aaagcaattg gttgggcatt acgcgattat agtaagaccg atgccgattg ggtaaaaaga
     7081 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     7141 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     7201 agattcaaac aaacaagctt tagatagcac aagagaagca ttggaaaaat tacgcaaagc
     7261 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     7321 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     7381 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     7441 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     7501 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     7561 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     7621 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     7681 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     7741 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     7801 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     7861 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     7921 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     7981 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagctttcg
     8041 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     8101 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     8161 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     8221 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     8281 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
     8341 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
     8401 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
     8461 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
     8521 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
     8581 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
     8641 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
     8701 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
     8761 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
     8821 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
     8881 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
     8941 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gactgcatta
     9001 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
     9061 ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa cgatgatcga
     9121 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
     9181 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
     9241 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
     9301 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa aagaagcaat
     9361 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
     9421 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
     9481 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
     9541 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
     9601 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
     9661 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
     9721 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
     9781 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
     9841 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
     9901 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
     9961 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    10021 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    10081 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    10141 ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa ctattgactt
    10201 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    10261 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    10321 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    10381 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    10441 aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt atcgatttgc
    10501 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    10561 atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg ccaatgatca
    10621 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    10681 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    10741 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    10801 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    10861 gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaattacaa
    10921 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    10981 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    11041 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    11101 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    11161 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    11221 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    11281 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    11341 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    11401 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    11461 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    11521 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    11581 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    11641 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    11701 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    11761 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    11821 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    11881 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    11941 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    12001 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    12061 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    12121 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    12181 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    12241 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    12301 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    12361 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    12421 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    12481 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    12541 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    12601 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    12661 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    12721 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    12781 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    12841 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    12901 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    12961 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    13021 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    13081 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    13141 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    13201 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    13261 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgacagacag gtaccttttt
    13321 cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    13381 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    13441 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    13501 aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac aagcagataa
    13561 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    13621 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    13681 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    13741 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    13801 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    13861 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    13921 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    13981 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    14041 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    14101 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    14161 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    14221 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    14281 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    14341 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    14401 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    14461 aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    14521 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    14581 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    14641 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    14701 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    14761 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    14821 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    14881 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    14941 actctgacta agattttact tcagctttac cgactgcttc gcccgggcga acaagtcctg
    15001 attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca ataatgtcag
    15061 ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg gtctctggtg
    15121 tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt tcaacagtat
    15181 ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca atggcatgtt
    15241 ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg ccgtttgacg
    15301 gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca tttacttcag
    15361 ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt gcaactgccg
    15421 taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt
    15481 gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc aaaattccct
    15541 gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc attgcgatga
    15601 tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg cctactccga
    15661 tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca gagccgacca
    15721 ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca ggttcagtta
    15781 cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt ttattcccac
    15841 gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat aaagcaatca
    15901 tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg gcattggtca
    15961 tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg gcactaaaca
    16021 accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag cttccgactg
    16081 tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac aatgaaaaca
    16141 atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag atttctagat
    16201 acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa ccaatcatct
    16261 ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg gaagctacac
    16321 tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt cctaatattt
    16381 gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga aaatggaaaa
    16441 ttgcttcacc gggtaaccac aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa
    16501 cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg aatccaagga
    16561 tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa
    16621 tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt tctttggaaa
    16681 ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca tcgtttccta
    16741 tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt atatcttcga
    16801 tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga gtcgcacaat
    16861 gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc tcggcatctg
    16921 cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc aagttgtttc
    16981 ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa agtacgagag
    17041 aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg aacattgaaa
    17101 tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat
    17161 gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga aagaatatcc
    17221 tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg tttcacgaga
    17281 aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa aaaaacaagg
    17341 aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg gattgactag
    17401 ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc atacgttaga
    17461 agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg gtgcgcctgt
    17521 ttggcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag acaccgatta
    17581 tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat cgatctatga
    17641 atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga tcacagtaga
    17701 agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt atgcagtagt
    17761 agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg agtcggttca
    17821 ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca
    17881 aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc acaaaggggg
    17941 catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa aagttatcac
    18001 ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc gggaaatatg
    18061 atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg ttattcagct
    18121 ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca ataacgaaaa
    18181 gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa gttcgaagca
    18241 aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat caaggtggtc
    18301 tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag atggatgaat
    18361 taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag agcggtacaa
    18421 tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta tttgaaagaa
    18481 acgctcccta gactcctttt tttcacaagg tgaaatgtgc tacaataaag ggaacgtatg
    18541 tgtgcaaaag aaagaggact agaatatgta tgaatatctg acaggtgtgg ttacctttat
    18601 caacccctat tacatagtag tagaaaccaa tggtatcggc taccagatcg cactaggaaa
    18661 tccttaccgc tacagtagca aactaaatca aaaaataaaa atttatgtcc atcaggtaat
    18721 acgtgaagat gcgcatcttc tgtatggatt tgattcgcta gaagaaaagc aactgttttt
    18781 gcgattagta agtgtttcag gaatcggtcc gaaaagtgcc cttgcaatca tggcaagcga
    18841 agaccactcc ggtttgattc aagcagtaga atccagtgat gtcacttatt tgacgaaatt
    18901 tccaggagta gggaaaaaga cagcacaaca aatgatcttg gatctaaaag gaaaattcgg
    18961 tgagctgagt atcgatacac cttttagtct atttgatgaa gcaaacacaa aagacgctac
    19021 agctctatct gaagcaatgg aagctctctc ggctcttggt tacagtgatc gagaaatcaa
    19081 acgtgtagaa aaacaattaa aagaagtaga taatcagaca acggatgagt atctacgcca
    19141 agcactcaag ctaatgatga aaaaataagg aggagaccaa atgacagatg aatacttgtt
    19201 atcaccagaa tctggcgaaa atgaactgag tttggaaaaa tctcttcgac cacagctgct
    19261 ttctcaatat atcggacaga gaaaagtcaa acaagaactt cagatttaca tcgaagctgc
    19321 cagaaatcgg gaagaacctt tagatcatgt tctgttgtac gggcctcctg gattaggaaa
    19381 aacgacgatg gccatggtca ttgctaatga gatgaccgta aatattcgta cgacgagtgg
    19441 ccctgctatc gaacgtccgg gtgacttagt agcaatcttg aatgaactgg aagcagggga
    19501 tgtcctgttt atcgatgaga tccatcgatt acctagagtg gtagaagaga tgctttattc
    19561 agctatggaa gatttctatg tagatatcat ggtaggacaa gggccaaccg cacatccggt
    19621 tcatttccct cttcctccct ttactttgat tggcgcgacg acacgggcag gtatgctttc
    19681 tgcaccttta cgagatcgat ttggcatcat ttctcatatg gaatattatg acgaacagga
    19741 tctgcaggag atcgttttac gttcttcgga tatttttcaa acagagatca ttgaagaagg
    19801 ggcattggaa attgctcgcc gatcacgagg aacgcctaga atcgctaatc gcttgctcaa
    19861 acgggttcgt gattttgcgc aagtccaagg agacgggaaa atcgatcgtc ctatagcgga
    19921 tcaggcactt actttgctac aggtcgatca ggctggattg gattatgttg accagaaatt
    19981 gttgcgaaca atgatcgagt tatatggcgg cggaccagta gggttaagta cgctttcagt
    20041 aaacatagga gaagagcgtg agacggtaga agatatgtat gaaccgtatc tgatccaaaa
    20101 aggattttta aagcgtacac ctagagggcg aattgctact gcttatgcct atgaacattt
    20161 tggttacgac tatcatatat gatttttgct tttagattgg aggaaaatga atgagtgata
    20221 caacacgtac gaaacaattg atcaaaaacg tattgattga tttgtgccaa acgcaatctt
    20281 ttcgaaaaat cagcgtccaa aaaatcgcaa acgaagcaaa aatcaatcga caaacatttt
    20341 attatcattt tacagataaa tacgatttgc ttcgttgggt ctattgtgag gatgcattgc
    20401 attatttaga tccacaatgt ctttcacttg ataattggga agagcaagca ttgaaaatgc
    20461 tgaaagcaat tgctgagaag aaagaatttt atgcaaatac tgtgctttct gacccagaaa
    20521 ttttacaaaa agagttttct caactgatcc aaaaatcatt tatcaagatt tttgaacaaa
    20581 tggatgaaga aagacaactt tctgcaagag acaaactatt ttatgctcgt tttttttctt
    20641 acggttgcag tggtgtatta gtcaattgga tcacgaatga atatgaagaa acaccgatgg
    20701 aaatcgctat gcagcttttc cgtttagcca aggatacgga attatattct tatcgtcttt
    20761 atgcaaaaga agagttggaa gaacagtaat agaaaaattt tttgttggaa aagcaataat
    20821 cgctaaaagg agatggggaa atgacaaaaa ttttatttgt ttgtttggga aatatttgcc
    20881 gttcaccgat ggcagaaggc ttgctgagaa aacgaatagc attagaagga agggaagcag
    20941 aattttttgt ggattctgct gctacaagca cgtatgaagt tggaaagaca ccgcaccctg
    21001 gaacgaaaaa aatattaaac caagaaaaag tagatatgac gaatatggtt gctcggcaaa
    21061 tcacacccca cgattttgaa acttttgatt ggatcattgg catggatcaa gaaaatgtag
    21121 aagaactgaa acgaagagca ccacgctcag cgcaggggaa aatccatttg tttttaagta
    21181 gcattcctgg aaaagaaaaa gaaaatgttc ccgacccata ctataccaat aactttgatg
    21241 agacatatca cttgatcaca gaaggcctga atcattggtg gaatatttgg attgatgaaa
    21301 ataaaataac aaacaatttg taaagttaac gctttctttt taaggcgatg ttagagcatt
    21361 tccttgtttt tttgtaaaat gttaggtaaa ataaaattaa ttaagtaaca ctttcttagc
    21421 taaaggagga gtgcattcga tgaacgaaga gacagtatcg ccaatgaaga agcttacgaa
    21481 gttagttact catacttcgt atcaactttt tgattctttt gtaaatgaca ctgaagaaca
    21541 tgctaagaat gtacgattgc aaggaaagtt tattcttgag ctgcaaaaag ctgctactga
    21601 acatagtcta gttatcttac aagtaagagc aaatcgtttg agtagcaagt atgaaacgat
    21661 ctcaggctgg gtggcgacaa aaacggtcga aaaagaccga gtgatgatcc gcctacaaaa
    21721 cgaagagcag cagatccgga tggttcctgt agaacaaatt gaaaaaataa caatcctttc
    21781 accagcaggg gaacaggaac ggttatcaaa ataatttata ccagatagaa aagcacgtat
    21841 gagcttgtac catcaatcat tactaatcaa aaataaccga atgctttaag ctgatatcag
    21901 taaaacggaa cgttttagga tgagtatcat agcatgacag cattcggttg attagttgaa
    21961 tcgattggat ggtacaagtt tttttgataa aaaaaggtcc tgctagccgg ggagctagca
    22021 ggataaggag ttaaaaatga aaaagtgttg ttagggttgt ttgttggtat gcttatataa
    22081 tagtcattag ttgtgaatat tttgtgacga aagttttcga aagttgttaa gttt
//
LOCUS       DAZUMI010000041        21993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000041 DAZUMI010000000
VERSION     DAZUMI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.041"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1167
                     /locus_tag="ACG81C_001338"
     CDS             202..1167
                     /locus_tag="ACG81C_001338"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5064509.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1266..2438)
                     /locus_tag="ACG81C_001339"
     CDS             complement(1266..2438)
                     /locus_tag="ACG81C_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064510.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2425..3570)
                     /locus_tag="ACG81C_001340"
     CDS             complement(2425..3570)
                     /locus_tag="ACG81C_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064511.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3584..4508)
                     /locus_tag="ACG81C_001341"
                     /pseudo
     CDS             complement(3584..4508)
                     /locus_tag="ACG81C_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4625..5704
                     /locus_tag="ACG81C_001342"
     CDS             4625..5704
                     /locus_tag="ACG81C_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5064512.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5701..6329
                     /locus_tag="ACG81C_001343"
                     /pseudo
     CDS             5701..6329
                     /locus_tag="ACG81C_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6439..6666)
                     /locus_tag="ACG81C_001344"
     CDS             complement(6439..6666)
                     /locus_tag="ACG81C_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5064513.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6769..9357)
                     /locus_tag="ACG81C_001345"
     CDS             complement(6769..9357)
                     /locus_tag="ACG81C_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5064514.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9377..10036)
                     /locus_tag="ACG81C_001346"
     CDS             complement(9377..10036)
                     /locus_tag="ACG81C_001346"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5064515.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10085..10588)
                     /gene="trmL"
                     /locus_tag="ACG81C_001347"
     CDS             complement(10085..10588)
                     /gene="trmL"
                     /locus_tag="ACG81C_001347"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5064516.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10809..11675)
                     /locus_tag="ACG81C_001348"
     CDS             complement(10809..11675)
                     /locus_tag="ACG81C_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5064517.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11784..12425
                     /locus_tag="ACG81C_001349"
     CDS             11784..12425
                     /locus_tag="ACG81C_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5064518.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12487..13848)
                     /gene="mgtE"
                     /locus_tag="ACG81C_001350"
     CDS             complement(12487..13848)
                     /gene="mgtE"
                     /locus_tag="ACG81C_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5064519.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13866..14765)
                     /locus_tag="ACG81C_001351"
     CDS             complement(13866..14765)
                     /locus_tag="ACG81C_001351"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5064520.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14767..15564)
                     /locus_tag="ACG81C_001352"
     CDS             complement(14767..15564)
                     /locus_tag="ACG81C_001352"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5064521.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15542..16246)
                     /locus_tag="ACG81C_001353"
     CDS             complement(15542..16246)
                     /locus_tag="ACG81C_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5064522.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16377..16952
                     /locus_tag="ACG81C_001354"
     CDS             16377..16952
                     /locus_tag="ACG81C_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5064523.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17070..17747
                     /locus_tag="ACG81C_001355"
     CDS             17070..17747
                     /locus_tag="ACG81C_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5064524.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17826..19634)
                     /gene="pepF"
                     /locus_tag="ACG81C_001356"
     CDS             complement(17826..19634)
                     /gene="pepF"
                     /locus_tag="ACG81C_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5064525.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19810..20808)
                     /locus_tag="ACG81C_001357"
     CDS             complement(19810..20808)
                     /locus_tag="ACG81C_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5064526.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(21034..21687)
                     /locus_tag="ACG81C_001358"
     CDS             complement(21034..21687)
                     /locus_tag="ACG81C_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5064527.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6492 a         4699 c         3814 g         6988 t
ORIGIN      
        1 cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct gctataataa
       61 ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca ttcaaaaaat
      121 agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat atcttgtttt
      181 attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa tccatcggca
      241 ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa aaataagcat
      301 attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat tgctgaaact
      361 ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat cgatactttc
      421 ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga tactttctat
      481 cagatggaag tagaattccc agttgcttat attccagcag gttctggaga tgattttgct
      541 cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt agcagctcaa
      601 tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata
      661 gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac aaatcattct
      721 tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt attctctatt
      781 ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg aggaaaacaa
      841 ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt tggcggagga
      901 atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa
      961 cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa aaaacaattg
     1021 aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc aacaacaccg
     1081 caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat cacaataacc
     1141 acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct acatctggaa
     1201 caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct ttttttacta
     1261 tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt acccaaaata
     1321 aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa
     1381 gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt attttgaaca
     1441 aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa gtattctgct
     1501 ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt gatcccaccg
     1561 ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat gatcggcaat
     1621 aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga ggtgtggatc
     1681 actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta actaatcaaa
     1741 ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg gtttcgttta
     1801 ctgatctctt tgcgattaaa tgctagattg atgacgctga aaccgctaaa aatggataga
     1861 aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt catccgctga
     1921 ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg tgtctgttcc
     1981 atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg
     2041 ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg tatcttgatc
     2101 gtcggcatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg taagataatg
     2161 tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc atccaatgta
     2221 acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg cgactcttct
     2281 tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc aatattttgc
     2341 gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt cgatttattt
     2401 gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc tttccatcca
     2461 agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat tggtataaaa
     2521 actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca agttgacgag
     2581 atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt gttccgaccc
     2641 catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc tatccttttg
     2701 caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa tcgcacacaa
     2761 cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt ctacttgata
     2821 gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg taaagcttgc
     2881 tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca agcgaatacc
     2941 atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca tcgtcccgta
     3001 gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca tattacgacc
     3061 tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg gcgcgagatc
     3121 aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta aaaattgatc
     3181 catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga gcgaaacaga
     3241 tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt cattcgcttc
     3301 tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga cttgttctaa
     3361 gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa ttccagtatt
     3421 aatcgtttcg agattgccac cttgatcaat ctcgccaaag gccgccataa acatcaatcc
     3481 taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca gcagtatttt
     3541 tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct ctcaaatcct
     3601 tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct atttttccgt
     3661 aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta tttagttcag
     3721 tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct attgttcgct
     3781 gacaacacgt tttctgggag taatgggatg tcgatcgtca aacgatccgc cgtccgtacc
     3841 atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc catgatcacg
     3901 atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac aatggttgct
     3961 ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg ggggtctaca
     4021 gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc acatgcgatg
     4081 ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac atatttttct
     4141 aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca
     4201 tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa gaccgcaacg
     4261 tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt cattgttgtc
     4321 ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca gttgatggca
     4381 gtcgatttcc cactgccgtt tggtcctaac agacctaaga cttctccttc ttgtacttgc
     4441 agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt atcaatttcg
     4501 ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa taaagtgcaa
     4561 gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa tgaaggagtc
     4621 tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta tctcattgct
     4681 tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga tcgccgtttt
     4741 attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc aagttattct
     4801 atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga cgatgcgagt
     4861 gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt tgttgatttc
     4921 gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc
     4981 cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt acattgtctc
     5041 caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag cattggccct
     5101 gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga
     5161 catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga
     5221 aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg ccaccgttca
     5281 cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat cgatctcttt
     5341 tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga ttgaaggaaa
     5401 cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca tcaaagaagc
     5461 cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt
     5521 gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa aaaattctgc
     5581 tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca
     5641 tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg agggacacca
     5701 atgataacag tagtcattat tgagatgatc catttgtgac gaaatcgtta caaaccattt
     5761 tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag
     5821 tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa
     5881 cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt
     5941 tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt
     6001 atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac
     6061 aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga
     6121 cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag
     6181 tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg
     6241 ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg
     6301 ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat
     6361 gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt
     6421 tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg
     6481 cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg
     6541 atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa
     6601 ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt
     6661 agccataaag taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa
     6721 agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt
     6781 tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg
     6841 gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc
     6901 tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg
     6961 gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg
     7021 tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata
     7081 agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt
     7141 caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc
     7201 ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg
     7261 aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg
     7321 tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata
     7381 agtgacctca tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt
     7441 tgccaagatg atacccgtga tttctcccat atctccattg aatacattca attcgggagt
     7501 attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc
     7561 cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat
     7621 ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt
     7681 atttttcgct ttttccacaa ttttagagat caagggttca atttgataag catcacttcg
     7741 gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat
     7801 ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc
     7861 cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga
     7921 gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa
     7981 taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc
     8041 ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg
     8101 atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt
     8161 tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag
     8221 tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc
     8281 tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg
     8341 cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc
     8401 tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat
     8461 cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt
     8521 tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg
     8581 gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt
     8641 atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct
     8701 cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat
     8761 tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt
     8821 cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc
     8881 catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt
     8941 tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat
     9001 agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa
     9061 tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata
     9121 tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg
     9181 ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg
     9241 gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt
     9301 ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg
     9361 aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg
     9421 tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa
     9481 tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg
     9541 tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat
     9601 ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa
     9661 tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca
     9721 gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc
     9781 ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga
     9841 taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa
     9901 catattttac ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag
     9961 aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg
    10021 taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa
    10081 ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt
    10141 gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga
    10201 catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt
    10261 ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat
    10321 tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta
    10381 aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat
    10441 ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat
    10501 gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta
    10561 atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt
    10621 ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac
    10681 tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat
    10741 aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc
    10801 tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg
    10861 tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc
    10921 tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat
    10981 tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca
    11041 cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc
    11101 tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt
    11161 tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca
    11221 gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta
    11281 gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat
    11341 tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct
    11401 tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt
    11461 ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa
    11521 taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta
    11581 tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc
    11641 aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc
    11701 ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag
    11761 tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta
    11821 ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg
    11881 gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag
    11941 atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg
    12001 aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag
    12061 tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg
    12121 aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc
    12181 ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg
    12241 gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct
    12301 atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac
    12361 atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt
    12421 tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt
    12481 gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact
    12541 cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag
    12601 acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag
    12661 catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag
    12721 aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac
    12781 aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc
    12841 tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa
    12901 aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc
    12961 taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc
    13021 attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc
    13081 aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt
    13141 aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc
    13201 gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc
    13261 attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac
    13321 atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga
    13381 gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt
    13441 ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact
    13501 taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt
    13561 atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt
    13621 catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag
    13681 ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc
    13741 atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc
    13801 ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc
    13861 tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg
    13921 aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct
    13981 tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag
    14041 ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca
    14101 actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct
    14161 tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata
    14221 tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca
    14281 aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa
    14341 agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg
    14401 taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat
    14461 tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca
    14521 tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga
    14581 atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca
    14641 ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct
    14701 ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac
    14761 tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg
    14821 ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc
    14881 ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat
    14941 ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt
    15001 attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc
    15061 gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc
    15121 tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc
    15181 ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata
    15241 actgatccga acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct
    15301 gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt
    15361 atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa
    15421 taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat
    15481 ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt
    15541 attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa
    15601 gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt
    15661 cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg
    15721 cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg
    15781 aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa
    15841 ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga taagaacggt
    15901 agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc
    15961 ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta
    16021 acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg
    16081 attttgttac aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat
    16141 gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata
    16201 cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta
    16261 cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt
    16321 tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga
    16381 cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca
    16441 tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata
    16501 ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc
    16561 aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct
    16621 ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag
    16681 tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc
    16741 ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt gatgaaagct
    16801 ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa
    16861 aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca
    16921 agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga
    16981 gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt
    17041 gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt
    17101 taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg
    17161 ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa
    17221 ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact
    17281 tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga
    17341 aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat
    17401 attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta
    17461 aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc
    17521 gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg
    17581 attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt
    17641 gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt
    17701 ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa aatacgtcga
    17761 ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat caaaaataga
    17821 aagccttata aacgatcgat caattcttct aattcattta gacgttgttc aaacatcgac
    17881 attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt cattacttcc
    17941 acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag tgcctctggt
    18001 tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt cgagtattgg
    18061 aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt aatttctggg
    18121 tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact atcacttagg
    18181 tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg ctcgaattca
    18241 gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg gttgagtaca
    18301 taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag gatattttca
    18361 ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac ataaggctgg
    18421 ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag cgtaaatagc
    18481 tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata gcttcccgaa
    18541 gaataagcat cgcttcgttt ccctttattt tcgacaacat cgatccaacg ttcagagaat
    18601 gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc taaagctttt
    18661 tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg tgtataaaga
    18721 tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat gtatcgatgt
    18781 aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact ttcaggaata
    18841 tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt cgctttaaaa
    18901 ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa ttgttcgtaa
    18961 acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct ttctagcaac
    19021 tgaccataca cgccatgaga taattggact atttcaccat tttctccttc aatcgttgga
    19081 aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc aaagatttca
    19141 cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg attatctact
    19201 atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa atattgccaa
    19261 atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc ttcactaact
    19321 ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt tccagtgtct
    19381 tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata aaccctcaat
    19441 acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc tttgtgtttt
    19501 tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc ttggtcgctt
    19561 gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg ttttggcaac
    19621 tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat caaaatagat
    19681 catagtctaa catccacatg tcacagcatc taacttttct actcattcat gtgtctgctt
    19741 ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc tagcctattt
    19801 ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa actgctgata
    19861 gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt ttatttggca
    19921 aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt acaacattcg
    19981 ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat agatccacga
    20041 tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg atactgtatt
    20101 cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac tcactttttc
    20161 tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt taaggtccct
    20221 actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag tctctgtaat
    20281 atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat gcactcctct
    20341 ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt gtccaagaaa
    20401 agaattacca agtatccacc atacttggta actgttattc aaataatttt gggttctttc
    20461 aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat tcgccaacaa
    20521 aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg gttctactga
    20581 ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg attctctttc
    20641 actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt ttttctttcc
    20701 ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc ctttttctgc
    20761 atgtttagca aaacagcact tattatattc attcattgca agcagcattt ttctccctct
    20821 gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa aaagctattc
    20881 tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc gtttggttgg
    20941 gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga ctgcgacaca
    21001 atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt tcgttcgctc
    21061 gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg gtgtacgatc
    21121 gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg cttggttgag
    21181 attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat acaaatcagt
    21241 ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa aatcttcaaa
    21301 agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt cttcctccgg
    21361 atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat caacagtaga
    21421 atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca gttctaatcc
    21481 gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact cctcttcgat
    21541 atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat tgccaagtaa
    21601 atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta cacggatcgt
    21661 attttcatta atatgttcca tttccatttt gctacacctc actttgccac aatgtttaat
    21721 atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg ctgttaagcg
    21781 aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa ctataacatt
    21841 tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc tatttttcgt
    21901 gcaatcatca tcttctacta cctttttata catctaatca agaatacttt ttgtaaaaac
    21961 aaaaaaactt ggaaaaacta tctttgtaat agt
//
LOCUS       DAZUMI010000042        21810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000042 DAZUMI010000000
VERSION     DAZUMI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.042"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1452
                     /locus_tag="ACG81C_001359"
     CDS             112..1452
                     /locus_tag="ACG81C_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5064528.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1670..2113
                     /locus_tag="ACG81C_001360"
     CDS             1670..2113
                     /locus_tag="ACG81C_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5064529.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2617..4035)
                     /gene="nhaC"
                     /locus_tag="ACG81C_001361"
     CDS             complement(2617..4035)
                     /gene="nhaC"
                     /locus_tag="ACG81C_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5064530.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4438..5661)
                     /locus_tag="ACG81C_001362"
     CDS             complement(4438..5661)
                     /locus_tag="ACG81C_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5064531.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5692..6531)
                     /locus_tag="ACG81C_001363"
     CDS             complement(5692..6531)
                     /locus_tag="ACG81C_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5064532.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6548..7228)
                     /locus_tag="ACG81C_001364"
     CDS             complement(6548..7228)
                     /locus_tag="ACG81C_001364"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5064533.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7230..8303)
                     /locus_tag="ACG81C_001365"
     CDS             complement(7230..8303)
                     /locus_tag="ACG81C_001365"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5064534.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8359..8597)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8640..8873)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8950..9621)
                     /locus_tag="ACG81C_001366"
     CDS             complement(8950..9621)
                     /locus_tag="ACG81C_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5064535.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9708..12356)
                     /gene="mgtA"
                     /locus_tag="ACG81C_001367"
     CDS             complement(9708..12356)
                     /gene="mgtA"
                     /locus_tag="ACG81C_001367"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5064536.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12698..12872)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13045..13482
                     /gene="ndk"
                     /locus_tag="ACG81C_001368"
     CDS             13045..13482
                     /gene="ndk"
                     /locus_tag="ACG81C_001368"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5064537.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13504..14370)
                     /locus_tag="ACG81C_001369"
     CDS             complement(13504..14370)
                     /locus_tag="ACG81C_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5064538.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14530..16623)
                     /locus_tag="ACG81C_001370"
     CDS             complement(14530..16623)
                     /locus_tag="ACG81C_001370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5064539.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17372..17995)
                     /locus_tag="ACG81C_001371"
     CDS             complement(17372..17995)
                     /locus_tag="ACG81C_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064540.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18154..19551)
                     /locus_tag="ACG81C_001372"
     CDS             complement(18154..19551)
                     /locus_tag="ACG81C_001372"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5064541.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19583..20554)
                     /locus_tag="ACG81C_001373"
     CDS             complement(19583..20554)
                     /locus_tag="ACG81C_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5064542.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20928..21353
                     /locus_tag="ACG81C_001374"
     CDS             20928..21353
                     /locus_tag="ACG81C_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064543.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6289 a         4501 c         3780 g         7240 t
ORIGIN      
        1 ttctgaatag ttgtaattgt ctgaaaattt tttatcatta gtcattgtta aatattgcta
       61 tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt tatggaagat
      121 aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt aggtggagca
      181 atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg cccttctgta
      241 ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa
      301 atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg cccttatcta
      361 ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat tatcaatatc
      421 atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca cattccagcc
      481 tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta ttcggtacgg
      541 ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat tatcttactg
      601 atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt tttccctacc
      661 cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg aatcatcagt
      721 tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat cacggtaagc
      781 gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat gggaagaatc
      841 atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg gaatacttta
      901 gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat cccatttgca
      961 gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa ttcgggtgtg
     1021 tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa
     1081 ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg taccagtgtt
     1141 ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg ctatctggca
     1201 ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc atcctttgta
     1261 ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc attagctgtc
     1321 ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac tgtattgact
     1381 ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg
     1441 acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga tggagctatt
     1501 gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata aacagtctca
     1561 tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat tgttctccat
     1621 gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa tgaaaaagta
     1681 ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta tgatgtactt
     1741 gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca tggcgttgat
     1801 gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt atgaaaacaa
     1861 aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt ccttgtttat
     1921 ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga ttcctcatca
     1981 ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag taaaaaaatt
     2041 agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa aaatgatcaa
     2101 agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag aagttatcag
     2161 cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt gtgaaaaagc
     2221 tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat ttttcgtttg
     2281 gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtaaaat
     2341 cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca tattttgcca
     2401 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga ggttgtgaaa
     2461 aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca catttttgta
     2521 taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt aaaagggatt
     2581 gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt gcgctgtctt
     2641 gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg tgaaggcact
     2701 gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt
     2761 aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca ctaagtagtt
     2821 gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa atcctgcttt
     2881 ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt gttcaccaat
     2941 aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg gtgtgttcgt
     3001 catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga tcaacaatcc
     3061 acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt cgattcctcc
     3121 gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc ctgattcgat
     3181 gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca ttcctactgc
     3241 tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg cacacagtaa
     3301 cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt ctagaacatc
     3361 agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta aaatcatgaa
     3421 taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct tgatgtgttt
     3481 gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata atggagacat
     3541 cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg gatttaagcc
     3601 tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact
     3661 ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa accactggac
     3721 actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt gtatccatac
     3781 agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg gtttgatgcc
     3841 attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg catacaacat
     3901 gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc cgacaacgcc
     3961 aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa aacttggtgt
     4021 tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta
     4081 agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa aataaaaaga
     4141 cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt
     4201 ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg tcaattaggc
     4261 ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc tactgcgtga
     4321 aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata
     4381 ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagatta ttttttacta
     4441 gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt gcttgattct
     4501 ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta gttctgctgc
     4561 aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt cttctgtgtg
     4621 tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta tgacttggct
     4681 gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta tttcttcatc
     4741 tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag gagtaacgat
     4801 gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc
     4861 agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt gccgatttcc
     4921 tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag ctactcctcc
     4981 gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat
     5041 tccattaaac acttggatca atgcttccaa agcatttact cctttttcag gtgctgctgc
     5101 tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta aagattctgt
     5161 cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat cctcaaaaaa
     5221 accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt cccctggggt
     5281 gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta tgctggcggc
     5341 taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac ctggtaaagc
     5401 atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat aacgtgcatc
     5461 aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt tttctaattg
     5521 tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat gttcgtggat
     5581 atctagagca agtgcttgat attctggtaa acgatattgg attagatcct tcaattggtt
     5641 tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt cttattttct
     5701 cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga ttactttgac
     5761 tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt tttcatcttc
     5821 ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg gttctaagaa
     5881 gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat tgatcactga
     5941 aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag aaatgtctag
     6001 attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg ccttatccac
     6061 tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg taacatcatt
     6121 agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag tgttagaata
     6181 aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc cgtgttcttt
     6241 attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg atccatctgc
     6301 taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat taatgttctc
     6361 ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa taatccctag
     6421 ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac accctgccaa
     6481 ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca ttttttctcc
     6541 tccactttca atgtgtaatt tttttaatca gttgattact gataaattga cttaaaaaaa
     6601 ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat cgattatacc
     6661 ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc atggtagtca
     6721 gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc aaaccttcta
     6781 acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct gccgcttcga
     6841 tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa
     6901 ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca actaagaaac
     6961 gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga atgatattga
     7021 tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta gggcgtgtca
     7081 ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct gtccaaaaag
     7141 acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa taaacattgg
     7201 gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac tttgatacga
     7261 ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat caaataatcc
     7321 aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact ccctaacggc
     7381 gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg atatttgctg
     7441 tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa ttccaccagc
     7501 catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg
     7561 tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg attgctgaag
     7621 atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc taccttgttg
     7681 cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa acctaactgg
     7741 cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg
     7801 gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc gattgctact
     7861 cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc ttccaaaccg
     7921 acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt tccagaatac
     7981 tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt gaaatgctga
     8041 aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag ctgactcaga
     8101 ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt catcacacga
     8161 actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc gcctttttca
     8221 atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt gaaacataca
     8281 gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc gtcctcaaga
     8341 aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc atgttcaagc
     8401 tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata tcggtcgcag
     8461 ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt tccaatagtc
     8521 gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta tcgtacttac
     8581 catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt
     8641 aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct caagcccaac
     8701 atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa tttgcctact
     8761 tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc ttctcaccag
     8821 ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat cgatattttg
     8881 ttgttgggat taatctacat gaggaaaact gaaaagtcaa gtacgaaatt caaattattt
     8941 tttctatatt tatttttctg tttttttatt taacatttca ataacagttc gaagatcttt
     9001 tccttccgca agaagtttca atttttcctt ttcttcgacg gtataatttc ggacaaaact
     9061 ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa
     9121 agaatcttcc actgcaaagg ctgaactagc ttctaatcct gttcgttcca aagctgtcag
     9181 ataaggaatc ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa
     9241 gtaatcagct aatttaaagt gatccaagat atctgttgct cttttcgtaa cagtggtcgt
     9301 agcaatgcct agtttcatat gacgttcttt taagtctgcc aatatttctt ttgcatatgg
     9361 ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg
     9421 gacttcctgt actttttcag ggctacctgc tgctttcact aaaatatcaa aactttgttt
     9481 ccagtttttt ccgccccaag cgtcaacgat ttcttttggg atatggatat caaattgctg
     9541 aaatccccaa agccagccat cacgatacgt tttttctgtg tcaactaaca aaccatctaa
     9601 atcaaatatt gcacctttca tctatacatt aatcctttcc aaaacaatac acatagttta
     9661 gcacgactgg tccaaaaaga acagaagcaa agaaccagcc ttctcgttta taagagttct
     9721 tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata agcgatgact
     9781 gtagcaaata accataacca gtaattgcca ggaagtggca tcaagcctaa ttcagcacca
     9841 aaacttgtaa aaggaaggac agaaccgaca ataattccta atgtagtgat aacaaacatg
     9901 gcaaatgttg cgttgctttg gataaatggt actttcggtg tacgcagtgc atggagcact
     9961 aaggtttgtg tccatagtga ttcgacaaac catcctgcat ggaataatgc gatgaaaatc
    10021 actcgctgtg aactatccaa tgtatggaaa cttccgccca ctacagcagg acaaataaca
    10081 aaatacatca acaagtaagt agtaatatca aatatcgaac ttgttggacc aaagtaaacc
    10141 atgaattccc caatactaga tgcattccat ttttttggct cgtccaaata ttctttatcc
    10201 atccgatccc atggcaagga aatgcaggaa acatcataga tcagattcaa aaacaaaagt
    10261 tgtaaaggca gcattggtaa aaatggcagg aacgtacttg caatcagcac ggaaaacata
    10321 ttgccaaaat tagaacttgc tgtagccttg atatatttca tgatattccc aaaagtttct
    10381 cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct
    10441 gcagattctt tcgcaatatc cacagctgta tctacagaaa taccgatatc agccactttc
    10501 attgcaggag cgtcattgat accatctcct aaaaatccga cagtatgacc atttttacgt
    10561 aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt ttacgaatac ttcatggtct
    10621 tcagctactt gtgctaattc atgatcactc atttccgaga ttttctcacc ggtaaccaat
    10681 tcattgatct ctagacctac ttgacggcac actgaacgtg taactaattc gttgtcccca
    10741 gtcagaactt ttacagctac accatgttct ttcagagctt tcaacgcttg tttcgttgtt
    10801 tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga ctcatctttg
    10861 actgaaaatt ctccaacgac gcttggattg gtcttttgtg cgacaccaat cactcgtaag
    10921 ccatcttcat taagtgactg tacttttgca agtacggctt cttttcgttc ttttgttaat
    10981 ggtgtaacag taccgccaag atcaataaat ttagaaatgt tcagcatttc ttcaatcgca
    11041 ccttttgtga tcatttgtgt tttaccagct ggatcagcaa ccacaacact catacgacga
    11101 cgttcaaaat caaaaggaat ttcatccact ttttgataat tgatcttatc tgtatctaaa
    11161 gtttctgttg cttcatcaat gatagccaca tctaacaaat ttttcagccc tgtttgatag
    11221 tagctattta gatacgcatg gcgcaaaaca cgatcatctt catttccttc acaatccata
    11281 tgatattcaa gaatgatttt atcttgtgtc aatgtccccg ttttatccgt acataacaca
    11341 tcgattgcac caaagttctg gatggcattc aaatttttga tcactgttcc ttttttggcc
    11401 atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt
    11461 agaccaactg ctacagatag tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc
    11521 ccattgatga cgataactgc tggagccatc aatgacataa atttgatcag tagcatggat
    11581 gttttatgga tgcctaattc aaagcttgat tgaattggtt tttcagatag cgtttctgct
    11641 acatgaccaa atagtgtttc atttccaaca gaaacaatga ttccttccgc acttccactg
    11701 atgacattac ttcccataaa gacaagattt tcgtaacttg tttcgctttg ctgatcgtct
    11761 tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc tgcttgggaa
    11821 atgaataaat cctttgcgga caataaacgc atatccgctg gtatcatatc ccctgctgaa
    11881 agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtgta ctttccatta
    11941 cgttttacag ctgccgttac tttcacaagt gatttcagtt tttcagccgc ttgattggaa
    12001 cgtacggact gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa gataatggct
    12061 acaccgaaaa gatctttatc tccaggagct gctaatacat attccgtgat gaatgaaata
    12121 attcctaatg cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa tatttctaca
    12181 atgaatggtg tttttttgcc atatgaaatc gcattttcac cgtaattttc tcttgaacgt
    12241 tcgatttgtt cttctgtcaa tccatttgct gatgcatgga atagttcaaa taattcttgt
    12301 ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt ttccatctca
    12361 atctctcctt tgtgtccaaa agacagctgt tctcgctgct tttggtcttt agctattcat
    12421 cggatcgata gagaaaaaac tgaaaggggc tgagaacaga tagataatag ccctggcctt
    12481 tcctctagat ccttgttttt cgaatgactg tcatccattt gttctcatct cctttactaa
    12541 gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tatttttcca attagaccac
    12601 ctccttttta tttttcaccg atggggattt gcggcaacaa aaaaacctct cgccaaatac
    12661 tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct cagatctcat cgttgagttt
    12721 tgacactgta caacgtaaaa agcagatacc tgcttttagc taccttatga aaagccttat
    12781 ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag tagcctatgt
    12841 ttgtatagga gcctcaccta acaaatctta ttcggttttc cttcgttaat ataacaacta
    12901 aaaaagagga tgtcaaatca attttccatg tttttcatac tttttttaaa atacacacgt
    12961 tatattcctg tctcacgcgg ttttatttgt ctcattttcc acctcccgct ataatagaaa
    13021 tatagtagga aacgaggtgc tgttatggaa agaacacttg ttattatcaa gccagacggc
    13081 gtgagacgcc atttagtcgg aagcatcatc caacgttttg aagcaaaggg actggccatt
    13141 gctgaaatga agtttgaaac tatgactcct gaattagcaa aagaacatta tcagcacttg
    13201 actgagcgca gtttttttga tgaactgatc gattacatga cttccggtcc tgtcgtttat
    13261 cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggttgg agcaacaaaa
    13321 gctgccgatg ctgttccagg tacgatccga ggagactacg cgctgccggg gactgaaaac
    13381 atcattcacg cttctgattc ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg
    13441 ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct
    13501 ggtctagttg ctgggttcag caatggccag cagttttttc tctaaggacc tccgttgatt
    13561 ctttcctaac atatattcga gcactgtgaa gaattcttgg tagtctttcg tcttttcttc
    13621 ccctaaagca gcagaaatca atctttgata ataatctgcc atcaatgttt catagatcac
    13681 tgtacttctt tctttatgtg ctaagctccg tttcatatct tcattcacac tacgtgcctt
    13741 tttcaaccga ccattttcta tataaaacga agtcaggttc attaaaaaag taaagagata
    13801 ctgctcatga ttgttcaact gtgctaatcg tcgcattctt tttttgattt tcaaataatt
    13861 gttctcaatg aaccgatcac tgaatatcca aggaaaatca gctagaatag atagttcaaa
    13921 ttgaccatag tctggtatct gcatcaagta ttcttcaatg attcggcatt cataacgtaa
    13981 aaattcaaat tctgtctcag atacttctgt tttttcgacc atgtgaagat tcaaaaccaa
    14041 ataatacaga tcttctgttt tcttaaaatg cctacggtta ttgctgatct ctttataagg
    14101 atcaaagctg tcttcttgta cccgatctag cattcgtctt tttttcttta actttattgg
    14161 ttcatccagc tggtttcgca tccattcata ttctgcaagt gtcatgttca ttcgctctaa
    14221 aaatgatctc aatgttacaa atgacatttt tttcactgaa tttgtctcaa tcccgactag
    14281 agaagtctta aaacagattc cttccgctaa ttgggactgt gtcaacttct tgttgactcg
    14341 aagtttcttg atcaattcac catcatacat aatagttctc ctttttaatt taaatttttc
    14401 cacattcaat aaatagatac gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt
    14461 tttttcataa aaaataccaa ccttcaagca ttagaatgtt gtctaactac tgaaggttgg
    14521 tttgattgtt tattgattat ttttttgctg ttcaacgtct gttcttgtta ctggatataa
    14581 gttggttgtt gttttattac cattttttga aaagacactt gtcgaatgtt cgcctttttg
    14641 cttttcgatt gcttcattac gttccgcttc atttttatag tcatattttt tcggatcaat
    14701 cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca gcttatctga
    14761 aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcat cgattcctgc
    14821 tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc ctaggacggt
    14881 gtatttgggt gtcacaaagt tcccatttcg aaaagcaact acttgatcat gctgagaaga
    14941 taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata gatgtagtaa
    15001 tgtcggcaaa acatcgattt ctccaccgta ttcatgattg actgtccctt tagtataacc
    15061 aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt catcccatgt
    15121 atctggatct ttaccaagag cgcttgctaa atctttatta tctgtattcg agatcccaaa
    15181 atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt tcaaatacgt
    15241 aaaaaattgt tccaatgatt gatccagata atgtgccgtt cgaacataat tatctactgt
    15301 gctattgcca gtattcaaag atggaaagtc gaaattcttg tcatccgtat aataaggcgt
    15361 gtggttggtt acggaaagaa atttcgtgta aaacggctgt tgtagatgct caagatattg
    15421 ggcagattct ctgaataaat ctttatctaa tatcccataa cctaacgttt cgtcggattc
    15481 atcaaaataa gatcgatcaa agaaattgtc ataacctaag tttttatata catgatctct
    15541 attccaaaaa ctagctacat tcccatggaa aacagcgctt gtatagcctt gcttttgtcc
    15601 aaggatcgct ggtgcggctt gaaagacatt gtctgaccca agttctgtga ataatgagcc
    15661 ttgaggtaaa ccaaatgtgc cagtttccaa catattttca gcatcacttg tctttccctg
    15721 tcctacttca tgaaagaaat tgtcaaagct gatcgttgct tgattctgga aaatactatt
    15781 taaaaacggt gtgacttctt gtccgtctac cttcatatta atcaaaaact gctggaaact
    15841 ttctaaatgg agaacgatca catttttccc tttagcgatc ccaaatgttt cgggatttgg
    15901 ctctgcataa tgtttctttg tataatctaa aacttcatca atcccattac tgcttgcatg
    15961 cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca taaatagtat acgcatcgat
    16021 tcctagatat ttgacaatat atgaacggtc aaatgttcgc tgcagcaatt gtggacggtt
    16081 ggcttcgctc agcgctagat ttcctgaaaa aataagccct gaaagacagg taacagcgat
    16141 agccatcggt ttgccgacag ggttgctttt gataggtatt ttcttgacct tcaaccaaac
    16201 cagtagccca atgaacacag cgatatccag ccagtagatg acatcatggg gcttcatcaa
    16261 agaaaaggag cttcctgaca atccttgcga cactttcgaa aagcctaata cagatttgac
    16321 tgtaatgaaa tctgtgaatt cccgataaaa aataatattc aagtacaaca aaagcgtatt
    16381 taaaatatca atgatcaaaa gaatagctaa cgctggcttt ggctttttaa tatacaaaga
    16441 aaggccaaaa aacaataagg tagtcgcaat tgggttgatc cacaagataa agtactggat
    16501 cgtaccttcc acacctaatg aaaaatctgc atagtaggca atgatcgtct tcagccagaa
    16561 aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg cacaattttt taagttgtgt
    16621 cacgtatatc acttctctct attcacattt tttcttattt tgctcctgaa aaatgaaaat
    16681 ttcctaaaaa agttgttatc ttaagatttt tttattcttt ctaaagatta ttccgtccac
    16741 ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac taatgaagag ataaaagtct
    16801 tgtggtgacc gcttcactcc acggtattca aaagccagtt tctcggtatt cgtacaaaaa
    16861 aagaggctgt gacaaaattc ttgtcgcagc tccttatttc ttgatgcagg acagcttttt
    16921 cacaacttct tccttaaacg gtgttctcct gccaactcct cggccacaag tcacaatatt
    16981 tataaaacat gagaagctgt tttttgaaat tgttccttgt ggctcagagt taaacggcag
    17041 gctcaagacc tcttgataca cggtgttcaa tcagctgacc ctcggtatta gattatgtca
    17101 ggacgctaat tttacaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata
    17161 agcccaacca aacgaaaaat ataaggagct attttttgaa attgctcctt gtggctcaga
    17221 gttaaacggc aggctcaaga cctcttgata cacggtgttc aatcagctga ccttcggtat
    17281 tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc taatactcag
    17341 gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc ataggattga tattgtcctt
    17401 gtgatgactc attcgtaata attgaggctt gattttcttc acttcccgtt atcgtttgtc
    17461 cactgccaga atctgaagga cttgttcctg tcgactcgtt tttttgagaa gagacagttg
    17521 ttttcttgac ttgttcattt gtctttttcc attccgctaa acgatcggcg accacttttt
    17581 tgtctacttt ttcagaataa gcggtgatca cttttccatt tttcaaacgg gctactttgt
    17641 cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc tttaaattga tattgtctga
    17701 gagaaatcgc actaagtacg tcatcgatat taataaattg attttcttct tttccttttg
    17761 gcaaataaat gtccaattcg cctttttcgt aattggcagt tattttatac tccgcccacc
    17821 aataagttga gaattctttt ttgaaattat ctactgattc tttgcttgct acgatttttt
    17881 ctgtttcttt ctgagcaaga tctgctgtca tttttcctga aaatgctgcg gataaaataa
    17941 gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg tctttttttc ctcatgcttt
    18001 cctccactcc tatttaattt tgactattct attatataca aaaaatgaaa tagaaagaca
    18061 aaaaagttga aaaagatcgt aaataattga acttcttttt cacaaaatgg taagaatgga
    18121 gaatttcacg catttgctct caactattct ttcttaagcc tcaaatccgt ttttatctga
    18181 tacgtctttg aaccaatgac ccgatttttt gattgttctt tttccatctt gtgctaagtc
    18241 tactgaaata aagccatatc gattcttata tgcattggtc cacgaccagt tatccataca
    18301 tgtccacata tgatagccta aacaattggc accttcttga atggcttgat ggacatattt
    18361 cagatgagat tggacaaatt cgatccgata atcatcgtgg atcatgccat cttctttgat
    18421 aaatcgtgct tcgttttcta cacccatacc gttttcagaa ataaagcagt cgatatttcc
    18481 ataatgatct cgtacattga tcaaaatatc atagattcct tgttcataga tctcccatcc
    18541 gcgataagga ttcattttac gaccaggcat ttcataattg tcgaaataat cctcaggcat
    18601 tggaccgttt ttagtatcga ttggtgtttc ttttgctttt acccggcgtg gctggtaata
    18661 attgatacct aaaagatcta ctgtattttc tttgataacc gtcaaatcct ctgcatggat
    18721 cacaggcatg agatcaagtt cttttactat ctcaatcaat tcacttggaa actcgccttt
    18781 tacagctgga tcaaggaatg aacgattgaa gaacgcatct gctatttgcg ctgctttaac
    18841 atcttcaggg tttgattcgt ctcttggata gcttggtgtc agattcaaga tgataccaat
    18901 aacaccacct tgctccattt catggtaaga tttgatggct aacgctgaag ccaacgcttc
    18961 atgatagcca acttggatcg cttttttcat atctgactct tctggataat gccatcgata
    19021 taaataacct gcttcgactg gaacgattgg ttcattatga gtaaaccatt tttttacttt
    19081 cgaaccaaac aattcaaaac aacttttggc atatttttca taggcttcaa ccgtttctcg
    19141 gttcaaccat ccgccttttt cttgcagata catcggcata tcgaaatgat acagattcac
    19201 aaagggttcg ataccgtttt caatcaactc atcaaaataa cgatgataga aacgaacggc
    19261 ttcttcgttg attgttccag attcatcagg tatcaaacgg ctccattgga tagatgttcg
    19321 aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc tcttgatact tcgtataaac
    19381 ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt tgcggttctt ttttgtacca
    19441 ataatcaaaa atgttctctc cttttccatc gcctgaaatc cttccttctg tttgtgggcc
    19501 acttgctgca gaaccccacc aaaaatttgc tggaaattga tattttttca tcaaagcacc
    19561 tctttttctt ttttagttcg ttttagttga ttccatgtat gatattttcc atcacaatca
    19621 ctttttcatt ttcttcctct tcttttttga ttcgttttaa cagctgttct gctgctcgtt
    19681 ccccgatttt atctgttggt ggcgtgatcg tgattttcgg aaggatactc agatcgtttt
    19741 tatctaaata atcatagtga ctgaacgcaa tatccttccc gattctgaca ccttcttgat
    19801 tgaaacattc gatggcacca atcgacatat tgtaattgct gacaaaaata gcagttgccc
    19861 ctttttctaa taatgctttt gccccgtaat agccgctttt tctagaatag tctcctgtaa
    19921 agacctctaa ttggatcgat ggatcatttt ggaccgcttc tttggctcct gctaaacgtt
    19981 ctcttgctac gtagtctgtt tgagtggcag caataaatcc gattttttta tgtccttgtt
    20041 caccatgatg agcgatcact tttgctacac tttctcgatc attcactaat attgtatcaa
    20101 tacctgctgg tccttctgga caattcaatg cgataactgg aatactcatt tttgtgagca
    20161 tttcaaatcc agaccactct tcttcggata agaagacaat cagcccatcg atcacatgct
    20221 tcatcaggta ttcaattttt tgttcaattt tttctggctg atcggcaaac ccgcttaata
    20281 ataagctata cccattttct tcagctactc tttcgatccc tgagacaact tccgacccaa
    20341 aacggctgga aatcccgttt atcaaaagtc caatagagaa gcttcgatta ttcttcaacc
    20401 ctttagcaat gatgttttct ttatagtcca acgctgaaat cgcttctgct atttttttca
    20461 tgttcttttc cttcagttgc tgaccattca agtaacggga aactgttccc actgaaacac
    20521 cagccatctt agccacatca cggatcgatg ccatacattc tcactcctgc cagttacatt
    20581 tgatttctct ttcttgttgt tcgattatga tgaccgcatt ctttgcatag tgttgcttgt
    20641 catcaatatc catataacta aagtcttcag tagagcaact tcctctcact attaattata
    20701 cttaaacaag tatttgaacc ggttcatttt taatatagca tttttttaaa agattgcaac
    20761 acttttttaa aacgctttct gaaagtggcg gattttttgc ctttttaaaa ttttattcta
    20821 aaatttactc tatttgtggg tgttttgtgc gtttttctct tccatccgtt tgaagtttta
    20881 cattccaatg gtataatttc tttatcctat caaatggggg aagtattttg aagaaattaa
    20941 tcgtactatt tattttgtct gcgggacttt tgacattgac tggctgtgat ttgtttggcg
    21001 gctctgacca atcctcggac gaaacaaccg attcttctgt cttaaaagcg gaaaaagcag
    21061 ataccaccaa cagtgtatat acgctaaaag tacgtaaaga tatggattta tccgcgggac
    21121 agttgacatt tacagataaa aaaatggaat ggacacgcac gtatcagaca caaggaactg
    21181 attcttctga atcatctgaa gaaaaaacag tgctgacaga tatcaagatc actaccaaag
    21241 atgacgaata tattatttca ggaaaagaaa ataaaaaaga aacaacagtc atatttacaa
    21301 aaatcggtaa ttaccgtttg aaggatgagg atggcaacat ctacagtcta tagtgccgtc
    21361 tgttggatct tcttttgttt ctccccttct tttctgctat agtaaagaag caagatgatt
    21421 gtaaaggaga cgaaatggat gatcgatagt cctgattatt gtacatgtaa taacaacaaa
    21481 aatatccata cagaatttaa tgaccaagga agcttgagca tatgcgacaa ctgtcacaaa
    21541 ctagtcaaag attcatttaa aacatttgaa tcttatcctg ttcaaaatta tgtaaaaagt
    21601 agaagaagat aaacaaatcc aagcaaaact cactatggct gatttttatg aacttcaaac
    21661 tcagcttctt cctttgatca agcttcatca gtaaaacaaa cagtggatca tttcagttta
    21721 caaatcaaag agtaaagatt tatttatttc aaatatttga atataaaata accaaacgat
    21781 agaaaggctt attctacatc acgtagtaat
//
LOCUS       DAZUMI010000043        21520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000043 DAZUMI010000000
VERSION     DAZUMI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.043"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..901
                     /locus_tag="ACG81C_001375"
     CDS             200..901
                     /locus_tag="ACG81C_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5064544.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1510..2526)
                     /gene="tsaD"
                     /locus_tag="ACG81C_001376"
     CDS             complement(1510..2526)
                     /gene="tsaD"
                     /locus_tag="ACG81C_001376"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5064545.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2523..2987)
                     /gene="rimI"
                     /locus_tag="ACG81C_001377"
     CDS             complement(2523..2987)
                     /gene="rimI"
                     /locus_tag="ACG81C_001377"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5064546.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2994..3536)
                     /gene="rimI"
                     /locus_tag="ACG81C_001378"
     CDS             complement(2994..3536)
                     /gene="rimI"
                     /locus_tag="ACG81C_001378"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5064547.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3520..4242)
                     /gene="tsaB"
                     /locus_tag="ACG81C_001379"
     CDS             complement(3520..4242)
                     /gene="tsaB"
                     /locus_tag="ACG81C_001379"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5064548.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4433..5413)
                     /locus_tag="ACG81C_001380"
     CDS             complement(4433..5413)
                     /locus_tag="ACG81C_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5064549.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5437..6921)
                     /gene="galT"
                     /locus_tag="ACG81C_001381"
     CDS             complement(5437..6921)
                     /gene="galT"
                     /locus_tag="ACG81C_001381"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5064550.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6933..7922)
                     /gene="galE"
                     /locus_tag="ACG81C_001382"
     CDS             complement(6933..7922)
                     /gene="galE"
                     /locus_tag="ACG81C_001382"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5064551.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8170..8337
                     /locus_tag="ACG81C_001383"
     CDS             8170..8337
                     /locus_tag="ACG81C_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5064552.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8399..10210)
                     /locus_tag="ACG81C_001384"
     CDS             complement(8399..10210)
                     /locus_tag="ACG81C_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5064553.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10207..10572)
                     /locus_tag="ACG81C_001385"
     CDS             complement(10207..10572)
                     /locus_tag="ACG81C_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5064554.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10735..11130)
                     /locus_tag="ACG81C_001386"
     CDS             complement(10735..11130)
                     /locus_tag="ACG81C_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5064555.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11148..12110)
                     /locus_tag="ACG81C_001387"
     CDS             complement(11148..12110)
                     /locus_tag="ACG81C_001387"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5064556.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12110..12322)
                     /locus_tag="ACG81C_001388"
     CDS             complement(12110..12322)
                     /locus_tag="ACG81C_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5064557.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12343..13041)
                     /locus_tag="ACG81C_001389"
     CDS             complement(12343..13041)
                     /locus_tag="ACG81C_001389"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5064558.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13061..13603)
                     /locus_tag="ACG81C_001390"
     CDS             complement(13061..13603)
                     /locus_tag="ACG81C_001390"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5064559.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13735..14739)
                     /locus_tag="ACG81C_001391"
     CDS             complement(13735..14739)
                     /locus_tag="ACG81C_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5064560.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14736..15725)
                     /locus_tag="ACG81C_001392"
     CDS             complement(14736..15725)
                     /locus_tag="ACG81C_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5064561.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15722..16528)
                     /locus_tag="ACG81C_001393"
     CDS             complement(15722..16528)
                     /locus_tag="ACG81C_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064562.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16694..17650
                     /locus_tag="ACG81C_001394"
     CDS             16694..17650
                     /locus_tag="ACG81C_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5064563.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17727..18245)
                     /locus_tag="ACG81C_001395"
     CDS             complement(17727..18245)
                     /locus_tag="ACG81C_001395"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5064564.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18333..18482)
                     /gene="rpmG"
                     /locus_tag="ACG81C_001396"
     CDS             complement(18333..18482)
                     /gene="rpmG"
                     /locus_tag="ACG81C_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5064565.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18710..19156
                     /locus_tag="ACG81C_001397"
     CDS             18710..19156
                     /locus_tag="ACG81C_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5064566.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19351..21246)
                     /gene="asnB"
                     /locus_tag="ACG81C_001398"
     CDS             complement(19351..21246)
                     /gene="asnB"
                     /locus_tag="ACG81C_001398"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5064567.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6478 a         4561 c         3704 g         6777 t
ORIGIN      
        1 atgtattatt gttgtttttt atacacaaat cattcgcttc aaaacaaatt attcaatacc
       61 attgtatatt ttttacataa taacaaatta tatttgtaaa gtattaaaac ttgtttctaa
      121 tgttggtatg ttaaaacttt acaaattctt taaaaaaagt gacaataaat atagtgaaca
      181 ataaggagag gactttttaa tgcgtgttaa taaaatcgat tttcactttt caaaactcag
      241 atatcaacct gattttgaac atttctcgga tgaagagttc caacagatca aggaacatac
      301 tgttcttcgt tcttataaaa aaggacagat ccttttcgac gatggtgatc ccagacaacg
      361 gttgtatatc ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa gtgcaactta
      421 ttactactat gactatgtga atacaaataa cctttttcct atgtcaggaa tgtttggaga
      481 agagacctac acttatacag ctcaagcgat gactgatatt gaaacattct accttcctgt
      541 ttcctactat gaaaaattag cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa
      601 aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa atcggattga cctctagcgc
      661 ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa gaattgggcg taccaacaaa
      721 agatggtcat atcaccattc cttatccgat caccttaaaa gaattagcag tgaatagcgg
      781 cacgacacgt gaaactgctg gacaagtaat caaacaacta cgtgattccg ggctgttaga
      841 ttatgacaaa aagcgtttta ctttctgtaa gacagctact actccagcta tgagagaata
      901 acgcgaacaa aaataagcag tgaccctgat aaatatcagg gtcactgctt attttcctcc
      961 ctaaaacttc tgaaagatcc ggcagtttta gatcactgga caagactcaa agattttcta
     1021 tgtgtgtttc agttatgtaa tattcactgt ttgcaaaaaa tactgttaga ttaactagat
     1081 gaagcgtctt gtcctcatcc gatctagatt attcgatatt cctcgacaat aaacgattac
     1141 tcagtgaggg caatcagagg aacggataat gataaataag ctgtaaaaaa tagcaaaata
     1201 acaataatga ttaaaagtat gtaaaaaata gggctttgac aaaaacgtgt cacagcccta
     1261 tttcgattaa acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac
     1321 atgaggagct gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga
     1381 actcttgatt aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa
     1441 aaacatgaga agccgttttt cctgggctgt tcttattact tgatgcaggg cagctttttc
     1501 acaacctctt tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa
     1561 atttcttttt tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca
     1621 aagtcgaaga gggggaatca ctagttctac ctcagggagt ttagctgcca aagcatcctg
     1681 caatccttca cgcaaccctt gattggccgc tacacctcca gctataatca gttgcttgac
     1741 tggataattt tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg
     1801 aaagctagct gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac
     1861 caaattgata aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat
     1921 catcgctcgc ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt
     1981 tccgcttggg taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc
     2041 gtcatctctt gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc
     2101 tgtatgtcct ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg
     2161 ggctgcatag atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca
     2221 agcaaaagcc ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata
     2281 agtgacagcg actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc
     2341 caagcataag gtgatttgtt ctacgtgatg gcggctagct acttcaggta ctacgccacc
     2401 aaaacgttta tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt
     2461 tctaacgacc gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc
     2521 attcatttca tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga
     2581 ttgcgataat atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt
     2641 cttgctgctt cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt
     2701 tccgtaatca gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca
     2761 acagcaatat ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag
     2821 atctggttat cttcagctaa aagcagataa tcagagtgag gctgttggat gtctgccaaa
     2881 aactgcattt tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg
     2941 cattctgcca agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca
     3001 ttttctaaaa gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt
     3061 ttgatcgttg ttgcttgaaa ccccaatttc cgataaagtc tttgtgcatc ttgattactc
     3121 atacgcactt ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg
     3181 tcaataagca gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg
     3241 atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca
     3301 aagataccta gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt
     3361 gtccatggca attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct
     3421 tctgtagtca attctcttag tacgtaacga cgatcgattt gttcgactgt tttttctgta
     3481 taaatcattt tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact
     3541 ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct gcttctactc gttttaaata
     3601 gcgaggaaga aaagcttgtt tctctgcaac tggttcaact aaagcaccta attgagccac
     3661 agttacacca cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat
     3721 ttcctttatt tcagagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt
     3781 cagcttttgt agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacagt
     3841 ttctaatcta ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc
     3901 aaacatgggc acaataatat tatccacacc gatgcaatta gccgcgatgg ttttcagact
     3961 agatactcct actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc
     4021 tatacgaagc cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt
     4081 tggacttatt tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg
     4141 gttgcgtttg atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat
     4201 cgccaaagtt tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc
     4261 tatgacattc tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa
     4321 ggcaaaacta gtacaataat acatgattat ctgctggcgg gcaaattttg ccccttcctg
     4381 aaacacaaaa aagctgcggt atcactccat tttcccacag cttttccatc ttttatttcg
     4441 ttgattctcg aataatcaac tctgtttcca caataatctt tcttgaaacc ggtgctttgg
     4501 aattacttaa ttgaagaatc gtctccactg ctagctctcc catccattcg gtataaactt
     4561 ttacagtaga aagcgagggc gttacatatt tcgctacact gacatcattg aagcccatca
     4621 cagaaatatc ttctggtacc actaaacctt tttcttgaat cgctttcagt gcgccaatcg
     4681 ctaatgcatc attagctgca aaaagcgcat caggaacatg cggattcgtt tttaaatatt
     4741 gcttcactgc ttgatatcct gactcgacag aaaaatctgc ttcgataatt ggaccttcgt
     4801 cgtagccttt ttctgccaaa acttgttgaa atgccataat tctgggatct tttagccgct
     4861 gatggttttt cttcgtatat tctgttccag caagcatagc gatatgttgc gctttatggg
     4921 agacaaagtg ttcaatgact ttttgaacac ttgacttaaa gtcgacaact agagagtcga
     4981 ttccttgaga agtgccgtca gaatctacca aaagcaaggc attttgggat cctttcattt
     5041 ctaataactg agcttcgtcg aattttccta gtgcgatcgt tccatctgcc ggaagatcac
     5101 taatggaatc ccacgtttct ttcaataaag taacgtttgc ttcttctgcc tttttttcga
     5161 ttcctaggcg aatagataaa taataaagat cttcaagctc ttccgtatcg tcgtaccact
     5221 ggatcaggcg aagcgtttgc ttagtatttg ctgcattttt acgatgtttc gtataattta
     5281 agcgttctgc tgcttcaaaa attttttgct ttgtggctaa gccaacggaa agatcaggat
     5341 catagttgag aactcttgaa acagtagcag gagaaactcc tgccaactgc gcaatgtctt
     5401 taattgttgc catttgtctc tcctcctttt ttacgtttat aaaatcgcag taaatcgttc
     5461 aaacgctgcc tgtccttttt catctcgctt gaatactcca gcgtcttcca gtacacgtgc
     5521 aaaaacctga cctacagatt tttgtacgac atcggtgacc gtatctttcg tcaaatcagg
     5581 atgttctttt tttaattgtt ctgcccacgg acgatgatat tcagccatct ggttttcttc
     5641 tcctaataag aaatgctcta cttctactag ttctggtttc aaacgaggtg gtaaaacagc
     5701 aagccccatt acttcgatca acccaatgtt ctcctttttg atatgttgga catctttatg
     5761 tggatgaaaa atcccatcag gatgttcttc tgaaacattg ttgtctcgaa ggacaaggtc
     5821 caactcaaag taattgtccc ttcttcgtgc aattggcgta atcgtatggt gtggtgtacc
     5881 atctgctgaa aaagctctga tagatactgt ttcatccgaa tatgctttcc atttttccaa
     5941 tactgagtta gctgcttcaa taagcaagtt tttctctgtt gattgcaaac ggatcacaga
     6001 catcggccat ttcacgactc ctgcgatgac ttgcggataa tcttctagtt tgataagttt
     6061 ttctatcggt gcttttgcca taggaaattc atgacgtcct gcctgataat gatcatgaga
     6121 aagaatcgat cctccaacaa ttggtaagtc agcattcgaa ccaacaaagt aatgaggcaa
     6181 gacttcagta atccgaagca gtctagcaaa tgtttccttt gtgattttca tcgggcggtg
     6241 ttgttctgat aaaatgatcg aatgttcatt gtaataagca taaggggaat attggaatcc
     6301 ccagctttca ccgtctagat tcattcgaat aatccggtga ttggtgcggg ctggatagtt
     6361 caagcgacct ctatatcctt cgttttccat gcaaagcatg caggtaggat agtctacttt
     6421 taccgcctcg cgttctgctg cgatttgttt tggatctttt tctggcttag ataaattgat
     6481 tgtgatttcc agttcaccat actccgactc tgccggaaat ttgatgtttt ttgcgatggc
     6541 tcttgttttg atgtagtcat ttttcttact taaattaaag aaatactcag ttgcttcctc
     6601 tgggcttttc gaatagtgct gtgcaaagaa tgcattgacg actgaaggtg gcggtgtgag
     6661 aaaatccatt aattgcgctt caaaaatctc ttgttctgct ggcaattctg aaagaacacc
     6721 attttctctt gccttagcga caagctgatc caataagtct acagattctt ccttaactgg
     6781 tcttacttca atgctttcta gagcatcttc tcctaccatc cctaaaagac gattctgtaa
     6841 atataagcgg tccatttcca tccatccgcc agcttcgatc gcaagtgtgg caaaatcaca
     6901 aatcgtttga ctgattgaca tatctcattc ccctattctt cataaccatt tggatggctg
     6961 acatgccaat cccatgctgt tttgataatc gctttgatat ctgtgtattc aggtttccag
     7021 cccagaatct ctctcgcttt ttcacttgaa gcgactaatc ggctaggatc tccagctctt
     7081 cttggtgcta tttctgctgg aatttctttt cctgtcactt ctctagctgc ttctagcatt
     7141 tccttgactg agtatccttt gctgctgcct aaattgaaga agttgctttc atttccttct
     7201 ttcaagtatt ccaaagctaa aatatgtgca gcaatcaaat cttctacttg aacataatct
     7261 ctgatacatg ttccatctgg tgtatcataa tcgtctccat aaacagccaa ggcttttctt
     7321 tgaccaagag ctacttgtaa aatgattggg acaagatgcg tttctggtgt atgatcttct
     7381 ccgatagatg catctgcttt tgctccggct acattgaagt agcgcaaagc gacatagcgc
     7441 atgccatatg cttggtcaca ccacttcatc attttttcca tcatcaattt actttcacca
     7501 tacggatttt taggattagc tggtgtatct tctgtgattg gagactcttt cggctctcca
     7561 taagttgctg cagtagaaga gaatacgatt ttattcacgt tgaactcgtg catgacttca
     7621 agtaagattt gcataccgta tacgttgttg ttgaaataca tcaatggttt ttcgacagat
     7681 tctcctacta aagagcttgc agcaaaatgg attactcctt caatcggttc cttttcaaat
     7741 acggaacgta gaaattcttt atcccggatg tctccttcat aaaaatgagc atctggatgt
     7801 actgcttgtt tgtgacctgt gagcaaatta tctaccacag ctacttgata ccccttttga
     7861 actagctggt ctacagcgtg cgaaccgata taaccggctc cgcctagtac taaaattgtc
     7921 ataaaaaaca ctcctttgat tagtaaaatt gtgtttgttg attctatata atttaaaaaa
     7981 cgaagtttac ttttatggtg aaaacaaaca aagtaaaatt tcgtaaattc atatggtaaa
     8041 tttataataa aacatttagt aaacattttc aagggttttt tgcaaaggtt tacaaacaaa
     8101 cgtaaaacta gccttttttt ctcttttgtt ccataataga tataacaaat aataaatgag
     8161 gtggattcaa tgaaaaaatt tgtatctgga atattagtag gaagtctggc aacagccgct
     8221 gccgttgctg gtttggttgc ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa
     8281 gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgta aacgcgtttc tcgttaataa
     8341 ttatttaaaa atacgcatga agactttcta aaaaacagat aagtcatcat gcgtattttt
     8401 atttgtcagt caatataagt tgtgctattt tatctgcctg tctcagattc catggatctg
     8461 tataaggtct agctactgct gcagttagcc aatccacacc ttcaacaggt attccatagc
     8521 aataaaagtc agactggatt tcccctgaag catcaatcac gcgattcgta cctcgctcaa
     8581 catctatagc ttctgaatga taacttcctg tattatctga aaatgaaaaa gggcgaacaa
     8641 gtttgttttc cttcaaattt tttatgacag gattacttga acgagaaaag cttgtttgtg
     8701 gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga gaaataacat
     8761 agcggttgtg cttttgctga atatcaaatg gcggttcgat caaatgaaaa atacccgcat
     8821 ctatcagtgc tgctaattct ttcgtccgaa tgattggcgg accgatcgtt aaaaagctat
     8881 ttagatgagt aaaccatccc catagtattt ctttgtattc cttagcagaa aaaacgtgcc
     8941 attcaatagc ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa
     9001 atgctcctgt gcaattgcct ttttccgctt cttttatctg cccttcaata aatattcggc
     9061 tagcttctga aaagggctca ttctgatagg caagaggagt ttctaaatac gaccaatccc
     9121 atctataagg gattagtgct ggatgctttc cgagacactc ctctgaagag agggagagaa
     9181 aatcatgctc aaaatttttc tttggaatcg gcaagtgata ttcttcgatg attttcttgt
     9241 aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc
     9301 ccgactgatg ccattgctcc aggttttctt ttgttagtaa ccgtggccat gccattgctc
     9361 cgccttgctt ttgatttctt cctcgagggt aataagggag cccccttctt gatccactgt
     9421 atacaatcgg ttccttgcct gatggatgat agaccagttt tcccttttcg ttctcgaagc
     9481 gccctcctct tgatactgta aacaatccga cataatcaaa aaaacttaag cctagacctc
     9541 ttaaaaagac attttcttta ggcggtatct tcgaaagatc tacatctgca ggattagaag
     9601 gaagctgata cagtaaaaca tttttctttg cgtagtctac ccacactctt tcttcttctg
     9661 tctcatcatt ttcccagtga ccgcttgcta aaacaagctg atcgatagaa tagctttgct
     9721 gatctgtctg taaaagataa ccatcttttc ttttgcttac tttttttact aaagaataaa
     9781 caaactgaat agaagctgga aattcttctt gcaactttga gaaaaaccag cgttgataga
     9841 gtccgtataa acatcgagta gattgctgat ctttttccag acgattgatt tcttctataa
     9901 ttagttgctt atttttggtt accgtctgtt cgatataatc tgtagcaggt cctttactcc
     9961 attgaaacaa gttgggtccc tgtgttacca atcctccact ggacaatgtt tcatctgtaa
    10021 aaagcgtcac ttgttgagaa acagaattca ttaataactc cggtggctga ttcaaccgcc
    10081 aaactcgacc tccaggacca ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct
    10141 gattattatg gagaatcctt tctgctacac ttaatcctct aggtccggca ccaatgatcc
    10201 ctattttcat ttgcatgcct ccatttcgat aaattttttc aataaaacag catggttctg
    10261 atgttcattt tttgcaccat aaacaaaagt aatgactgga tgttctttca taagttgact
    10321 gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc aattcttgct ggtatttctt
    10381 tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg
    10441 tgcaatttct ttcaaccaca gatctaactg cgctttttct tttgaaattc ctcgtggcca
    10501 gaatctatcc acgagtattc tatagccatc ctctttttca gcatcaaggt aaacacgttt
    10561 taatttgatc atagttgttt ccttctttct accttttcgc ttagcttata tatgtagata
    10621 atagcacaaa ataaaaaaaa tgaacggcac aactatccat tgcgccgttc atgtgatctt
    10681 ggtcaaaatc taatgattga ttcggttttt aaagctgtca tacaatcatc tgttttatac
    10741 ttttttactt ttgatttttc cagaccatcc gtcatatcca cccttaagta aataaatctc
    10801 ttgataacct gctttacgta atttatttgc tgtacggatg ctcaaacttt tcttttgatc
    10861 gtaaatatag acaggctggt cttttcgaat agatcctaat gaatctttca aaacagtgta
    10921 agggatgttt cttgcaccta aaatatgccc agcatcaaac acatcttttt cgcgaacatc
    10981 gattacttgt gcttttctca ttccttctcg aaattcttct tctgtcaaag tcgttgctga
    11041 acgtttggcc attactcgta gatagacttc ccaaaggatg attgcaagca caatcaatag
    11101 caaaatgata ttaattaccc ataaaaccat gtaatgcctc cgttttttta agcaaatttc
    11161 aaggctaatg aggcagcacc aatgacacca gcttcattac caagctctgc taatttgatt
    11221 tttgttgatt ctcttacttg cgggaaagta aattctttaa aatatttctc tacacggcta
    11281 cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca cgatacttga cggatttaat
    11341 gtattcccca agtttccgca agctaaacct aagtagaagc atacgcgatc aacaaccatc
    11401 aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat ctttacttgt tacttcttct
    11461 ccgttgtcta gcattgcttt cagttttgaa tcccctgcgt attcttctgc caagaaacga
    11521 cctaggcgta ccacacctgt agcgctagat acagtttcta aacatccgcg tttaccgcaa
    11581 gtacattcga acccttcagg atccacagtg atatgtccga tttctcctcc agctccgctc
    11641 acgccgtgaa tcaaatttcc gccagcaacg atacctccgc caacacctgt tcctagagta
    11701 acgaatacaa cgtctggatc gttttctcca gcacctttcc atcgttcacc taaagcagca
    11761 acattcgcgt cattatcgat agcgaatttg attcctgttc ctgcctcgat cagttttttc
    11821 acaaattgaa cttctgtcca gtttaagtta tacgcaccaa taaccgttcc agcttcgctg
    11881 tccacgcttc ctggggtacc catgccgatt ccgataaagt cttcggcttt catattatat
    11941 aaattcaaac gatgattgat tgattcaatg atttcaggaa cgatatgctt accatcatcc
    12001 aaaatattgg tatcgatgct ccatttttgc tgaatctctc cttctggtgt taaaattgca
    12061 aattttgctg ttgttccacc taaatcaatc ccaataatct tcttgtccat tacaaatttc
    12121 ctttctttcg gctttcttca attcggtgct ctctttttaa tatgcgccac gctgtcatgt
    12181 atgtatcatg atcaatcaaa cggctatcat acagattacg caattcgata gtcattaatt
    12241 caatgtcata gatgcgtgca cccacataga taaagatgcc aaattttttg aacagctgct
    12301 gtacatcata taagctctcc atctttaagc acttccttca tattatacaa gtaaaccata
    12361 tttttttaaa cccagtaaaa tacatattac aaaaagaaat acaaaaacta gtgtagaaag
    12421 aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa cattccctaa
    12481 cactaatccg ccaattagcc caccgacatg tccccaaaca tctacagagg tatccagaag
    12541 accaaaaaca aatgtcaaca caataaataa aagaaaatgt ctgactagct gctggatcgc
    12601 tgggttatgt ttaaaatgaa accctaagat aaacaacgca ccgaacaaac cgaatatcga
    12661 agtacttgca ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc
    12721 cattatcccg ctgaacatat aaatcaagaa aaaacgccaa tggccataga ttgcctcgat
    12781 ttgctgcccc ataaaataca gtatcagtga gttcattacg aaatgcatca gtccaaaatg
    12841 gatgaagatt ggtgtcacta aacgccacca ttcattgaag tgaacaagat aaggaccata
    12901 cattccggca taataaggga tctcaagtcg cggaaataat tccatcatga ggaagaccgc
    12961 tgtctgtatc cctaaaaaag cataggtcca taacggtcgt cttaaccatg cacgcaattt
    13021 tatttgctgt tgataattca tgaaaacctc ccatttcatt ttagtcaata aacaattgtt
    13081 ggatcttctg atcaaagctc tccggttgcc attgttcatt caattgttca cgaaaaacaa
    13141 ggctacatgt ttttttatga aaattattta aataccggtc gtaataacca ccgccaaaac
    13201 cgattcgata accggctcta ttgtagataa tacctggtac aatgaggaga tcgatcatct
    13261 ccggcaaata gagcttctct gattgtggct caagaacacc aaaactcgaa cgtacaagaa
    13321 cggactgttc atccatttca taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa
    13381 ctattttctt ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt
    13441 cattcgataa agtcgtccct atcacttttg ctgacttcca gtactcgctg gcgaatagct
    13501 gtttatagat tcgctgttct ttttcctttt tcttgccact tttggccaga tttttcagct
    13561 tttcgattcc tttccggcgt aatcttactt tcaattggtc caaaaactca cccttccttc
    13621 tctcataaaa tggtatagga aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat
    13681 aaatagaata aaaagaagac ctagaaatga ttggccttcc ttttttaatg aatttcaact
    13741 gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga aaatatggcg caccaaaaag
    13801 ggcaatcaaa attcctgtag gtacttcccc acttggtaga atcactctag caaccatatc
    13861 tgcactaagc agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc agttattttc
    13921 tttacgtacg actagcttcg cagcatgagg agctagtagt cctacaaaag ataaactacc
    13981 tgtcaccgct acactaccgc ttgccaaagc aacagctaac aacaaaagga ttttttgact
    14041 tttagccacg ttaaggccta atccttgcgc gcgttcttga ccaaatgata gcaatacaat
    14101 cctctgtgca taaagtcctc ctagcggaat taagataacc gcccatggaa gcaatgccca
    14161 aacataaggc cagctcgcac cccagattga tcccgctaac catgcattca caaatccata
    14221 attttcctta gataattgga tcgtgcatag caatgttaat gcagaaaggc cggcattaac
    14281 ggcaataccg gacaataaga ttcgattcat acccaaaaag ccatttcgat cataggccgc
    14341 aagatagaca cagcaagcag ctaaaacacc acctagaaag gctacaacta gcaatagaaa
    14401 cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc ctgctccggc
    14461 attaatgccc aatatactag aatctgccaa ttcatttctt gttactcctt gcaagatata
    14521 accagaaacg ccaagacaag ctcctgctaa taaactgatc agtaatcgag gaactcgaaa
    14581 ttctctaaca atcagcgcca aagaagatga ttctttccct atgaacgtgg caaaaagctg
    14641 atccaggtaa ataaaaggcg taccaatcat taaactacct atagacagga tgacaagtac
    14701 aaaaagcaag aaatagaacg tccaatatat ttttttcacc gttgttccct ccttacttgt
    14761 atcagtaaaa acggaacgcc aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg
    14821 ggattgatca tcctcgctgt aaaatccgcc aacataacca atccaccgcc aactaaaaaa
    14881 gagacaggta gaacataacg atagtccgtt ccaaccattc cgcgaacaac tggcggaacc
    14941 aacagcccaa taaatgaaac aggtccgact aacgaaacgg caatggctgc taacactgca
    15001 actagacaga aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt
    15061 gtttcactca aatgaatcaa agatattgct tttcctaatg aaaaagaaac caaaacagca
    15121 agtatatata aaggaaacaa tactgctaat tgtttccatg tgatattggc agctccgcct
    15181 acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata
    15241 gcagtaaaaa aagaactaat agctacgcca gctaaaatca gctgtaaagg attcatgccc
    15301 attttcgaaa actgcaaaaa accaaatacg agcaaagtta cagcaaaaga acctgccaat
    15361 gacattagaa acacagaaaa atcagaagct ttaggccaaa aagcaaaact aagggccatt
    15421 gccaacgaag cacccgcgtt gattcctagt aagcctgaat ctgctaaagg atttcttgtc
    15481 actccttgca tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc
    15541 acacgaggca aacgaatatt tcgtatgatt tggtgacttt gttttgatct atcaaaatca
    15601 aacaacgcct gccaaaaatc tgctttttcc acagtcgctg cgccatttga aagagcaaca
    15661 aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga gtctattttt cttcatagtc
    15721 ctcataattg atcctcctgc tcttttggca acaaatcata ggttagaaaa actggtttat
    15781 ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa tatttttttc atacactctt
    15841 ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt accgtttttc attgctaata
    15901 aataatctga aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag atcgttgttt
    15961 gtttcatttg attgatttca gtcaataact gtaggatctc taattggtgt gccggatcta
    16021 aaaatgttgt cggttcatcc aagatcaaga tatctgtttc ctgagcaagc gccatagcga
    16081 tccagacacg ctgggtttgt ccaccagaca gcgttgcgat atcccgcata cgaaatccag
    16141 ctagacctgt agcatccaac gcccactcaa ttgcttcatg atcttgcgtg tccatgccac
    16201 tgaacagttt ttgataaggg tatcgtccgt aagatactac ttcttctact gtcatacctg
    16261 tagggtgttc actagtttgt gctaataatg caattttttt tgcaagttct tttgaactgt
    16321 atgtagagat atcagagtta ttgacaatga tctttcctga tttcacaggt agtatacggg
    16381 taaaactttt caataaagta gattttccac ttccatttgg cccaatcaaa ctagtgattt
    16441 tcccttcagg aatcgtaaca gacatgtttt caatgatttt tttgttttcg tagccggctg
    16501 caatgtcagc tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa atcattctca
    16561 attatgacgc tgtttcaaca tttcgtcaag cgctactttc atatctatag ccaaatgaag
    16621 cggttgacag cgttcgaaat atcaggaata ataaccaatg agaaagattc tcaattatgt
    16681 taggaggaaa gaaatgaaat taaaaacaaa agtattcaca ttatctagca tattgctgct
    16741 ggctggagta ctgagcgctt gctccccctc ttcagataaa gaaacagcta caactgccaa
    16801 ttcgacgact tcatccaata gtactcatac agtcacagat acacttggac ataaagtaga
    16861 gattccaaac gcgcccaaac ggatcatcgg cagttactta gaagactatc tgattgcact
    16921 aggggaaaaa cctatcgccc agtggactgt tggcagtggt tccattcaag attatctgca
    16981 agatgattta aaggatgtcc caacgatttc ttatgacctt ccttatgaga aagtgttaag
    17041 ctttgagcct gatttgctgt tgatttcttc ttccgctact gtcgaaggcg ggaaatacga
    17101 acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg tcacatggga
    17161 agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag aaaaaatcat
    17221 ttccgattat caaaaatcca tcaagaaaac tcgagaagaa ctagcagata aaatcgagaa
    17281 caagacagct gcagtgttgt gggtgaccaa caactcagcc tttatggtca gcaaaaatcg
    17341 gtcaagtgga agaatcgtat acgacgatct ggaatttggt gttcctgatc tagtggaaga
    17401 ggtctccaaa aaagccactt ctgactggtc tgctgtttcc tctgaaaagc tagctgaatt
    17461 agatgctgac tacatcatcc ttgtgaacag tgacaaagat gctgccatgt tcaatgaacc
    17521 aagctggaag aatctaaaag cagtgacaga caatcatgta ctgcaattcg gacctgaatc
    17581 tagttggcta tataacggac ctattgccag tcaggaaatg gtcaatgata tcaaagaaaa
    17641 actaaattga ttttaactaa aaaaagcatc ttcacgatta gtcgtgagat gctttttttc
    17701 ttattaacaa actagacgca tagattttat tcttcttttg aagcaaccct attttttact
    17761 ctttcccatt tttgaaaacg atgggcatat agattttctt gatccgttat cccctcatac
    17821 tcctctataa gggcgaagtc tgaaaaatca atattcccaa tatacgtatc gccttcaaac
    17881 gtgctatcga tcaatgttcg ccagatccga ccagtgtaag gaagaagttc ttgaaaaatc
    17941 cgactaccac ccgaaacata gatatcttct gagatcgttt gactatacga caaaagttca
    18001 tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa aatttggttg cgttgtcaac
    18061 acgatgatat ggcgataagg aagtgaaaga tcccccattc cttcataggt ctttcttccc
    18121 atcactaaaa ttcgatccat tgtatgttcg cgaaagaaac gcatatcatt tggcaatcgc
    18181 catggtaaca aaccattttt gccgatcaga ccatttttat cttgtgccca catggaaata
    18241 aacatattat ccgctcctta tgattttaga tgttttgaga taaaaaaagt ctcaattctt
    18301 tgaccagcaa ggaattgaga actttaaaaa cattattttg tttcacggtg caaagtaact
    18361 ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat caggattgtt acgtttgttt
    18421 ttgcttgtta gatagttacg ttctttacaa gaagtacact ctaaagtgat gtttacgcgc
    18481 atgtttttcc ctcccatttt tacaagaatc ctctaagatg actctcagac ttgattatct
    18541 taccatgatt ttttctagat tgctagtgtt tttcacgatt ttgttaagta aaactacttg
    18601 acgataaata caacggtttt acttctcaaa caaaatcttt ttctttttgt ataaataccc
    18661 ttacacttgt ttatatagat tatgtttttt aataaagggg gatactaata tgccatggaa
    18721 catgaaggac tttcctgcat caatgaaaaa cttagataaa ttgacaagaa aaaaagcaat
    18781 cgacatcgca aatgcattat tagatgaagg gtaccctgat aaccgtgcga tcccaattgc
    18841 cattgatcaa gcaaaaaaat ggtatgaaga tgctagtgaa tcagaaaaac gaactttcga
    18901 aaaagaaaaa aatccaagca agacggatga acacgatacg aatcctcgtg ctggaaaact
    18961 gcttgattca gatgtgatcg tagaatatga ggaagaacaa tggatcgtga aatctaaagg
    19021 ggcaaaaaag gcaagcaacc attttgatac aaaaaaagaa gcaatcgaaa aaggaaagca
    19081 agtagcacaa aacaaagaat caactttgtt catttataaa aaagatggga caaaagaaaa
    19141 agaaatcgat tattgaacat atgtaaaggg gttgtgacga aaatcgtgtc acaaccccct
    19201 tatgttcgaa acaatggtgt tcctctgcca actccgaacc ataagtcata atatctataa
    19261 aacgggaaga gctgtttttt gaaattgttt cttatcactc agtgttaaac ggtaggagaa
    19321 aacctctttt ttattgatat ttgattttca tcaaacagtc acatagtcga ttgcttctgc
    19381 ttgcggaatc gtttcatcta caaagtagac tttataccat gtcaagaaac tgaagattgt
    19441 ccagattttt ctttgctctt ctttacgtcc ttcgtgatgg tcatctagca acttcatgat
    19501 tttttcttga tcaaaaaatt cttttgcaaa atctgcttcg aataatgctt tgacttgttc
    19561 gtagccgtgt tcttctttca accaagcttt gattggcaca gggaatccta atttcacacg
    19621 attcgaccat tcttccggaa gatgtttgtt cgcagcttgt ctgaaaatat cttttgtgtt
    19681 gttttgattc aataaaaact tacttggtat ttgttgagcc aatttcatga cttcgatatc
    19741 caataaaggt actcggactt ccaaagaact tgccatagaa agtttatctg cctttaacaa
    19801 gatatctttt ggcatccatt gatgaagatc gacatattgc atcttattga cttcatcttt
    19861 gattccttct gttttctcgt aatgaacagt catgatttca tccacagatg gtgctgtttg
    19921 atattttggt gttaacactt ctgatgcttc tgattcttcg aacactcgag catgaccgat
    19981 aaaatattct tttgcaggag caagtgattc atataaatgg atccgtccat ggaagttttt
    20041 catttttccg attttacgtc ctaaattata gcgtgttcct tttggtaatt ttttcaatcc
    20101 ttgagcaatc acacggatga attttgaatt cgtatggaat ccatacgttt ggtatcccgc
    20161 aaacagttca tccgcccctt ctccagattg gactacccgc acactttgtg aagcaagatt
    20221 tgctaaaaag tacaaaggta cgcaagaagg atttgaatca ggttcatcta agtgatactg
    20281 gatcaaaggg aatgctttga acgacatgtc tccgtcgatc actgctgctg tattatctaa
    20341 gtccaatagt tcggttagtt ttctggcttc aatcgcttca ttataggttt tatcatcaaa
    20401 cccgattgag aaagaatgat ctggtttcaa tacagaggtt acataactgg aatcaacccc
    20461 gctggataag aaagaaccaa cttctacatc actgaccgta tgtgcttcaa tagaagcttg
    20521 aacggtttcg tcgatttttt cgatccattc ttgtcgggaa tgcgtttctt ttgtttcaaa
    20581 atctgcatcc caatattgct gaatgtctat ttttccatct ttatacgtat aataatggcc
    20641 ttcaggcaaa cgatagacat ctttgaagaa tgtttcgccg tttaatggag aatattggaa
    20701 agtcatataa ggtttcaacg cttctttgtt caattctttg ttaaaatcag gatgaggcag
    20761 aaaacttttg atttctgaac caaacataaa tgttccgttc atttctgcat aataatatgg
    20821 tttgatccca aagtggtctc ttgcaccgaa taactcattc ttttcattat cccaaatagc
    20881 aaaagcaaac attccgcgga tcttttgcaa taattctgtt ccccattctt cgtaaccatg
    20941 caaaaggact tctgtatctg catgtgtttg aaaaacgtgg ccagctgcaa tcaattcttc
    21001 tctcaaaggc tggtaattat agatttcgcc attaaaaata atgatctttg tccgatcttc
    21061 attgtaaata ggctgagtac cgccctctaa gtcaataata cttaatcttc tgaatcctaa
    21121 agcaacatgt ttatcgatat attctcctga actattaggt ccacggtgaa cgattcgatc
    21181 catcattgta ttaataattg tttttttgtt gtccttgtcg ttaacaaaac ctacaatacc
    21241 gcacatttaa ctttcacatc ctaatataaa attactgttt atttcattca tatgcatctt
    21301 atcatagaat aaatagcgga cgaatatttt ctctcttaca atttcgtcac gtgaaaacaa
    21361 aagatgaaag tttctgtttt tacagaaaat aaagtcccaa tatctaattt accatgtttt
    21421 cttttttgag gctaccttat tctcttcgtg ttaaaaaaat attagtggat gctatttcca
    21481 aaaaaaccag caaaaaaaga cacgacctaa agatcgtgtc
//
LOCUS       DAZUMI010000044        21302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000044 DAZUMI010000000
VERSION     DAZUMI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.044"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG81C_001399"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG81C_001399"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5064568.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG81C_001400"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG81C_001400"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5064569.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG81C_001401"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG81C_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5064570.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG81C_001402"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG81C_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5064571.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG81C_001403"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG81C_001403"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5064572.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG81C_001404"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG81C_001404"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5064573.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG81C_001405"
     CDS             complement(8053..8523)
                     /locus_tag="ACG81C_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5064574.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG81C_001406"
     CDS             complement(8569..8994)
                     /locus_tag="ACG81C_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5064575.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG81C_001407"
     CDS             complement(9061..9330)
                     /locus_tag="ACG81C_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5064576.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG81C_001408"
                     /pseudo
     CDS             complement(9341..10936)
                     /locus_tag="ACG81C_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG81C_001409"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG81C_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5064577.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG81C_001410"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG81C_001410"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5064578.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG81C_001411"
     CDS             15402..16376
                     /locus_tag="ACG81C_001411"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5064579.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG81C_001412"
     CDS             16546..17946
                     /locus_tag="ACG81C_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5064580.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG81C_001413"
     CDS             complement(18024..18746)
                     /locus_tag="ACG81C_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5064581.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG81C_001414"
     CDS             complement(18825..19331)
                     /locus_tag="ACG81C_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5064582.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG81C_001415"
     CDS             19454..21253
                     /locus_tag="ACG81C_001415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5064583.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLFQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5926 a         4668 c         3839 g         6869 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctaat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttttca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ct
//
LOCUS       DAZUMI010000045        20923 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000045 DAZUMI010000000
VERSION     DAZUMI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.045"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG81C_001416"
     CDS             591..1280
                     /locus_tag="ACG81C_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5064584.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG81C_001417"
     CDS             1267..2475
                     /locus_tag="ACG81C_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5064585.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG81C_001418"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG81C_001418"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5064586.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG81C_001419"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG81C_001419"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5064587.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG81C_001420"
     CDS             complement(6578..7273)
                     /locus_tag="ACG81C_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5064588.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG81C_001421"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG81C_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5064589.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG81C_001422"
     CDS             complement(8472..8663)
                     /locus_tag="ACG81C_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5064590.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG81C_001423"
     CDS             complement(8684..9574)
                     /locus_tag="ACG81C_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5064591.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG81C_001424"
     CDS             complement(9561..10328)
                     /locus_tag="ACG81C_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5064592.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG81C_001425"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG81C_001425"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5064593.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG81C_001426"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG81C_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5064594.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG81C_001427"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG81C_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5064595.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG81C_001428"
     CDS             14899..15282
                     /locus_tag="ACG81C_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064596.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG81C_001429"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG81C_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5064597.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG81C_001430"
     CDS             complement(16226..17044)
                     /locus_tag="ACG81C_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5064598.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG81C_001431"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG81C_001431"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5064599.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG81C_001432"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG81C_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5064600.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG81C_001433"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG81C_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5064601.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG81C_001434"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG81C_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5064602.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5982 a         4257 c         3836 g         6848 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgcggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gagtcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa tggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggtgatttc ctgtgtattc cgtctcctaa ctattggtat atctatattt
    17941 tttatccaca gatgcaagag gaaatctatt gatttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgcctcgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atatcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttatg cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatta cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattggctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatcctga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcattg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aag
//
LOCUS       DAZUMI010000046        20842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000046 DAZUMI010000000
VERSION     DAZUMI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.046"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            474..1853
                     /gene="brnQ"
                     /locus_tag="ACG81C_001435"
     CDS             474..1853
                     /gene="brnQ"
                     /locus_tag="ACG81C_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5064603.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(2009..2455)
                     /locus_tag="ACG81C_001436"
     CDS             complement(2009..2455)
                     /locus_tag="ACG81C_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5064604.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2482..2658)
                     /gene="rpsU"
                     /locus_tag="ACG81C_001437"
     CDS             complement(2482..2658)
                     /gene="rpsU"
                     /locus_tag="ACG81C_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5064605.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(2820..3248)
                     /locus_tag="ACG81C_001438"
     CDS             complement(2820..3248)
                     /locus_tag="ACG81C_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5064606.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            3340..4200
                     /locus_tag="ACG81C_001439"
     CDS             3340..4200
                     /locus_tag="ACG81C_001439"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5064607.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(4284..4634)
                     /locus_tag="ACG81C_001440"
     CDS             complement(4284..4634)
                     /locus_tag="ACG81C_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5064608.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(4650..6377)
                     /locus_tag="ACG81C_001441"
     CDS             complement(4650..6377)
                     /locus_tag="ACG81C_001441"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5064609.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(6590..7516)
                     /gene="trxB"
                     /locus_tag="ACG81C_001442"
     CDS             complement(6590..7516)
                     /gene="trxB"
                     /locus_tag="ACG81C_001442"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5064610.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(7661..9094)
                     /locus_tag="ACG81C_001443"
     CDS             complement(7661..9094)
                     /locus_tag="ACG81C_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5064611.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(9094..10497)
                     /locus_tag="ACG81C_001444"
     CDS             complement(9094..10497)
                     /locus_tag="ACG81C_001444"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5064612.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            10727..11629
                     /locus_tag="ACG81C_001445"
     CDS             10727..11629
                     /locus_tag="ACG81C_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5064613.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(11670..13556)
                     /locus_tag="ACG81C_001446"
     CDS             complement(11670..13556)
                     /locus_tag="ACG81C_001446"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5064614.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(13653..14504)
                     /gene="licT"
                     /locus_tag="ACG81C_001447"
     CDS             complement(13653..14504)
                     /gene="licT"
                     /locus_tag="ACG81C_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5064615.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(14962..16746)
                     /locus_tag="ACG81C_001448"
     CDS             complement(14962..16746)
                     /locus_tag="ACG81C_001448"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064616.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(16746..18497)
                     /locus_tag="ACG81C_001449"
     CDS             complement(16746..18497)
                     /locus_tag="ACG81C_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5064617.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(18639..18863)
                     /locus_tag="ACG81C_001450"
     CDS             complement(18639..18863)
                     /locus_tag="ACG81C_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5064618.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            19114..20700
                     /locus_tag="ACG81C_001451"
     CDS             19114..20700
                     /locus_tag="ACG81C_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5064619.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         5896 a         4473 c         3701 g         6772 t
ORIGIN      
        1 tctaggtatt tttcttgttt caaaaaaaga aaacaaaata gttaaaatat tcttgcattt
       61 caaaaaaaac aggtgtatta ttacgtcaac aaataaacat acagcaaagc gtcgaggaaa
      121 acagtaggct attcaacgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa
      181 tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct
      241 ccattatcag gctagcggat aactttatca aaaagcgtcc gtgaatgaag aagctttttt
      301 aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc
      361 ttttttttat attttaaggt ggtggttttc atggaatctg atattagtgg cattactaat
      421 tgctgcaacg tttatatcgt caatcaaaat gataattatt ggaggaaaaa tttatggaag
      481 aaacaaaaac ttattcatgg aaacaactat tgatggtcag ttcacttatc ttcgggatgt
      541 ttttcgggtc aggtaatcta atctttcctg tccacttagg tcaaatggct ggcgcaaact
      601 gggtcagtgc tggcgtagga tttgccatct caggcgctct tttcccatta ctagctatct
      661 tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat
      721 ggtatgctgc cttgttctta atccttgttc atttgacgat cggaccattt ttcggtacac
      781 cacggacagc tgctactgct tttgaaatgg ctgctaaacc atttttgcct gaaaaatata
      841 attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg
      901 tccatcccac acggttggtc aaatacgtcg gaaagtatct aaactccttg tttcttgcat
      961 tattagcaat catttttgtc gtttctttat tccacccaat gggaaatcta aatcaatctg
     1021 ctgcagatac ttaccaaaca aacgctttaa caaatggtgt cctagaaggt tataacacgg
     1081 ttgatgcagt agccttgctt gccttaagtg tcacttttgt ccatgcagtc cgtgaccttg
     1141 gtttcaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg agcgttgcgc
     1201 tagaagtcat gatctatttt ggtcttgttc ttctcggtgc gatgagtttg aataagatgt
     1261 ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact
     1321 tcggcgctag cttcttagga gtgatggtca cattaggtgt cttcactacc gctatgggac
     1381 tcgttgtttc ttttgcacaa gatttccata aactgttccc taaagtcagt tatatgacat
     1441 ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa tgctggtttg gacaatatca
     1501 tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg ttgattttgc
     1561 tttccttaac agcaaaattt ttccaaaaga caccatttgt ctatcaagta acgatgttgt
     1621 ttgctgctgt tcccgccgta ttggatatgc tggcaaattc gcctgcttta gtgagtcaac
     1681 aaagagttgt agcaagcatg ctcgaatttt atcatcatca cgttcctttt gctgcattgg
     1741 gacttggctg gatggtccca acgttacttg ggtatgctgg cagcttactc ttctattacg
     1801 cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa taaccagatc
     1861 aaacacaatt taacataaga aatattaaac aggaaaaaaa cagctagatg aaagaatcct
     1921 attctttcgt ctagctattt tttagatctc ttttttacga gcacaaaaaa actgcggaca
     1981 atgattgtcc gcagtttaaa tgataaaatt aagattgtaa tagttctttc acaatggcat
     2041 ttacttggtt tccatctgct tttcctttga ctttaggcat gatcgcaccc atgactttgc
     2101 caaattcttt cattgatgaa gcaccagttt gcgtgattgc ttcttgaaca agttgtcgaa
     2161 tttcttcgtc ggaaagttgt tccggcatgt atttttcgac tatcacaatt tcacttttta
     2221 ctttttcagc taaatcgtca cgtcctgctt cttcaaactc atgcaaagaa tcacgtcgct
     2281 gtttcatttc gcgagacaac accgttaatt cttcttcttc tgtcaagtcg tgaccttctt
     2341 taatctgctc gttttgtata gatgacttga tcattcggat gacttgtaaa gattccttat
     2401 ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct acttagtagt gacacactac
     2461 caactccaat tcatcacaga actagaattt tttgcgtttt ctagctgctt cagatttttt
     2521 cttgcgtctt acacttggtt tttcgtagaa ttcacgttta cgagattctt gtaaagtacc
     2581 cgcttttgac acggaacgtt tgaagcgacg aagagcatcg tcaagtgatt cgtttttgcg
     2641 aacgactgtt tttgacatgt taattccctc cctccgagct taaaaagtaa ccgtaattgt
     2701 tattgaaata tatctcaaaa aacaagactg tcctgtaaat attatatcta atgctgtttt
     2761 gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa tgttatttta tttaaatcct
     2821 tattgacaat tttcacatct tccgaaaatc tcgaatctat gcccttcgat cgtacatcca
     2881 ccgagctgtt cttcgaaata attcattgga cacatatgga tttctttcgt tgttccgcac
     2941 accgtacaga taaagtgatg gtgatggccg atttcccctt gacagcaatg aaaacgaaac
     3001 ttcatctctc catttaaatc ggtttcttcc agtaaatcga tttctgcaaa atcatggaga
     3061 tttcgataaa tcgtatcgta acttactcct ttgaattcct gattcatata gtcaaaaact
     3121 tctttagcgg atacgtagcg attgcgcttc actaaataag ttaacaatgc ttctcttttt
     3181 ttagtatatt taaaaccgtt atttttcaat gtttctagtg attgttcgat caacgtctgt
     3241 tttttcatga cttattcctc caaatcaaaa tgattacgaa gtacacattc tagagtatgg
     3301 tataataaac ataaaaaaaa tgcaagtgag gttttatcca tgtcttccga ttctacagaa
     3361 atcgttactt tttttgtcat ctccgactct gctggagaaa ctgcgacaaa actagcgcaa
     3421 gccactatgg cccaatatcc tagcgttgag ttcaatttat tccgcagaac atttgtgaca
     3481 gatgagaaaa cattgatgca ggcactgcaa gatgcactag aagaaaaagc tcttgtcctg
     3541 cacacattga tcaatcaaga attgatcgag atgacgcgtt ctttttgtga agaacatcaa
     3601 ttatttagtt ttgatgtgat gactccgccg atatcagaaa tcgagcgctt aactggtatc
     3661 aagcccattc ggcaacctgg cgcccttcat ttattgaatg agaactattt caaacggatc
     3721 aaagcaatgg aatttgctgt aaaatacgat gacgggaaag atcctcgtgg gtttctagaa
     3781 gcagatgtcg ttttgctagg cgtatcccga acttccaaaa cgcctttgag cttgttttta
     3841 gctaacaaaa atttaaaagt tgccaatcta cctttgatcc cacaagcaca tattcccaaa
     3901 cagctttggg agatcgatcc taaaaaaatc gtgggactga cgaataaccc agatatttta
     3961 aacaatatcc gtaaagaacg gatgcgctct tacggattaa atcctgatac tgcttattcc
     4021 gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg atctttatga aaaattgggc
     4081 tgcgtagtaa tcaatgtcgc ttctttatct attgaagaaa cagcttcgct tattttgaat
     4141 gctttagacc tagaagacca tagttattat ggaactgaaa ccaccgaaga aacagactaa
     4201 caatttttct ttaaaaaaac tggactgtgt cagcgaagaa gattcgttga cacagtccag
     4261 tttgtctcta tttgcaccta tcgttattga tgttccacat gtttgagtgt ctggttcaaa
     4321 atatccagta cgtgagaatc atcaagacta tacaaaatcg cttttccctc acgatgggac
     4381 ttgacgacat ggttttctct taataaacgc agttgatgcg atacagcaga ttgttccatt
     4441 tccactgctt ggatgattgc tgtcacattt tgttccccgt ctttcagata cagcagaatt
     4501 ttgagacgag tggggtcact caacattttg aatagttggc tgactttctc tacttttgtt
     4561 ttttgcgtgt ctggcaatcc attcacctcc aacttgaaaa aagactgact tctaaagttt
     4621 accacttttt ccatcagtct caagtttttt tattcttctg tcatcttttg gataatcgtt
     4681 tcataagcgg ctactttttc agctgcgtct tctgaagagt ttccttttac accgatataa
     4741 aatttgattt tcggctcagt tcctgaagga cggatagcga tccagccatt ttcttccagc
     4801 gtatacttca atacgtcaga tggcggtgtc gttagttttt ctacgtttcc ttcacggtct
     4861 gttctcgtca actctttgaa gtcttctgtt tggatgactt ttgctccggc aaattctgtt
     4921 ggtgcttctt cacggaattt ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg
     4981 ctcattgtca cagaaatcgt tttttctgcg tagtagccat aggtttcaaa aatcgtttgc
     5041 aagccatcat acaacgtttt gccttgtttt ttatagtaag ctgctacttc tgctaataag
     5101 agcaatgctt ggatcgcgtc tttgtcgcga acgaaaggtt taactaaata accgtaactt
     5161 tcttcgaatc cgaacataaa agtatgggaa tgatcttctt caaattgttg aattttttca
     5221 gcaatgaatt tgaatcctgt caatacattg aacatggtag ccccatagtg ttccgcaatt
     5281 gctgtaggta attcgctaga aacgatcgat ttcaataccg ctgcgttttc aggtaaagtg
     5341 cctcgcgatt ggtgtgcttc caaaatatat tgagcaaaca gtgctccgat ttggtttccg
     5401 gtcaatactt cataggagcc atttggcaaa cgaacggctg cacctaaacg gtctgcatca
     5461 ggatctgttg caatcagcaa atcagcgcct tctttctcgc caagtcgtat cgcatactca
     5521 aaagcagagt gttcttctgg atttggtgat ttgactgtag tgaaatctgg gtctgcgaca
     5581 gcttgttctg gaactagtac gaattgttcg aatccagctt gtttcaacgc acgttcgccc
     5641 aacatttttc ctgttccgtg aagtggtgtg tacacaagtt tcaaatcttt ccccattgtt
     5701 tccaccaatt catgatcaat cgttactgct ttgatttctt ctagataagc ggcatccact
     5761 tcttctccga tgatcgtgat caatccgcta tgttcagctt cttcgtcaga taatacttca
     5821 acttttaacg gattgctgac ttcacggaca tatttcgtca atgcatctgc gtctgcagga
     5881 ggcatttgtc ccccatcttc tccgtagact ttatatccgt tgtaagctgc tggattgtgg
     5941 gatgcggtta ccatgattcc cgcaaacgtc ttcaaatgac ggactgcaaa agaaagctct
     6001 ggtgttggac gtagactttc gaatacataa gatgggatat tgtgatgtgc taatgttttg
     6061 gctgcttcca tggcaaattc tggtgattga tggcgtgagt cataagcgat agcgacacct
     6121 cggcgttttg tttcttcgcc ttgtgtatcc atgaaacgtg caagtccttc tgttgcttgt
     6181 cttaccgtat agatgttcat tcgattgatc ccaggcccta agattccccg catgcctgca
     6241 gtaccgaatt ccaacggtgc ataaaaagca tcttcaagtt tttcaggatc ttcacccaga
     6301 tcatttaatt gtttttttaa gttttcttct aaattttctt catgtaccca ttgttcatac
     6361 acttgttccc aagacatgaa aataacacct ctttctgaaa tatggttctt aataagtata
     6421 gcattcactt attagatatg aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat
     6481 tcttttatca agcacggttg caagtattcg cataaatcgg attcagtaga tttcacgcga
     6541 aaaaaaagag taaaagaaca attaaaccgt tcttccactc tttttaaact tatgcagttg
     6601 tttcttctaa agattccaag tatttataca cttgttgtcc agcgatccca ccatcaccaa
     6661 cagcagtagt gatttgtcgc aaatcttttt cacgaacatc tccgatggca aaaacgcctg
     6721 gtatattcgt cttcatttcg ttgtctgttg gaatccagcc agcttcgttc gtcaaccctg
     6781 cttctctaaa tggtgcagtt aatggatcca gtccaacata gatgaatact ccgtctgctg
     6841 ccaaatcaaa ctcattgccg gttttgacat tacgtgcttt cacacctgtc actaccattt
     6901 cattcccaac gatttcgtca acgactgtat cccacataaa ggaaattttt tcattcgcaa
     6961 atgcacggtc ttggatgatc ttttgtgcac gtaattcatc tctacggtgg acgatgacta
     7021 cttcagaagc aaattgtgtc aaatagatgg cttcttctac tgctgagtct cctcctccga
     7081 tcactactag acgtttgtta cggaaaaacg caccatcaca aactgcacag taagaaacgc
     7141 cacgtccagc aaattcttcc tcgccaggta cgcctaattt acggtggata cagcctgttg
     7201 cgataatgac tgtttttcct tcgtaagttt tatcttcaca aataacttct ttgtggctgc
     7261 cgtgatcctt gatatcttgc acgattccat aagcattttc tgtcccaaat cttgaaacac
     7321 cttcatacat cttataagct agatcaggac ccattattga atcgaaacca ggatagtttt
     7381 cgatttctgc cgtattgttc atttggccgc caggggcgcc ttgttcgatc atcaatacgg
     7441 ataagtttga acgtgaagca tataaagcag cagtcatccc agcaggacct gcgccaataa
     7501 cgattacatc atacatatgt agattctcct ttacttaatc aagtaagcat actttacagc
     7561 caatgcgaaa aaaagtctat tgttctgctt gcgtttctcg aatagcttta ttatactcga
     7621 aaaatccgct gaaaaacaat agacttttat aaatcattga tcaatcattc gaaagatttt
     7681 ctccatttga atcaattact tttttatacc agttgaatga tttcttcttc gaacgggcta
     7741 atgtgccatt tccttcgttg tcacgatcga cataaatgaa tccgtacctt ttcttcatct
     7801 cacctgtacc agctgatact aaatcaatac atccccaagt cgtatagcct aatagatcga
     7861 ctccatctaa ttctactgca tctttcattg cctggatatg tgctgccaga tagtcgattc
     7921 gatagtcatc ttccacataa ccattctcat cgggcgtatc gactgcacct aagccgtttt
     7981 ctacaataaa tagtggtttt tgataacgat cataaatatc attcatcgtt attctcaagc
     8041 ctaatggatc aatttgccaa ccccattcac ttgcttccag atatggattt ttgacagaag
     8101 caaagatatt tcctgcagtc tgttccaaga gttcgttgtc agtagtcgaa actcgagaag
     8161 aataataaga aaaggagata aaatcaacag tatgttcttt cagcaatgcg gcatccccag
     8221 gttccatttt gatctgaatg ccatttcgtt ccatttcttt caatgcataa gcaggatatt
     8281 ctcctcgtga ctggacatca atgaagaaat aattttctcg atctgatttt ctagaagccc
     8341 aaacatcttc aggattgcaa gtgtatggat aatatgctcc tgctgccagc atacagccta
     8401 ctttgttctc cggatctact tcatgagcaa tcttcgttgc gatggcactt gcaaccaact
     8461 catgatgtgc cgcttgatat ttgatttgtt cttcattttc tccttcttca aaatacagcc
     8521 ctgcgcccat gaatggtgca tgaaggatca tattgatctc attgaatgtc aaccaatatt
     8581 tgacaagtcc tttatagcga ttgaagatga ctcggcacag attttcataa aaaccaacga
     8641 gttctctgga acgccacgca ccatattctt tgatcaaatg catcggacaa tcaaaatgtg
     8701 taatcgtcac aagcggctcg atcccgtact tttgacattc tttaaagaca tcctcataaa
     8761 atttcaaacc agattcattt ggctcttttt catcgcccat tggaaaaata cgactccaag
     8821 caatggacat ccgataagtc ttgaacccca tttctgcaaa taaagcaata tcttccttat
     8881 aatgatgata catatcgatc gcaatttttg ctggataaaa atgattttca tcaaattcaa
     8941 acattttctt ttttccagta atgacaggaa aacggtcttc tccgattggc acaacatcta
     9001 cgttcgctaa cccacgtccg ccttcatcat aaccgccctc gcattgattg gctgctgtag
     9061 ctccgcccca taaaaaatct tttctgaatc ccatcatact tcctccatta catgtgtatt
     9121 gcttgataat tctcttgcca gttcatcttg gacttcgatt ccttctgctt ttaaccggct
     9181 gattgtttcc ttgaattgcg gtaaatactg ctgatgtgca ataaacaact cattcatgat
     9241 ccgttttgct gtctttcctg atcgtataag cggattgatc gtaaacgctt gtaaagctgt
     9301 tccataatca ccagtcacag ctgcttcgat cgtcagcaat tccatattct tcattacttg
     9361 caaccagcct ctttcagcag caggaaggga accaaacgct acatttcgtg cgccttgtgc
     9421 gccaacatag gcagtgactt ctactacaca atctgccggc agatcaggaa cagctccatc
     9481 attctttgta gataccacga tctgtgtttc tttatttcca tagatcgaag caatcgtttc
     9541 acaggcagca tctgaataat aagcaccacc tcgtttttgc aattgttcag gtttatgatc
     9601 caattctgga tctttgtata attcaaacag ctctgcttct gtttctttca cttgctgcgc
     9661 tcttgtacca atcgtttgat attcttctaa agcatgttcc agcatttctt cttcacggta
     9721 gtaatagcgg tggtagccac aagggatcat gttcatttgt tttaattgtt ctttgaaaaa
     9781 cgggatatca aagatatttt tcggcaatcc attgtcttca tcgtataaac gatcaatcaa
     9841 ttctgccgtg cggtcatttc ctttattatc tgctactttg tgccaatgga agtgatttag
     9901 accggcaaac ttgtagatca gctcttcttt tttcttgcca agcattgttg gttctgtcat
     9961 catcgccatt actggcacat tgcataatcc tatcacttta tcccatttgc catatctgat
    10021 aactgcttct gtcaccatcc cgcttgggtt cgcgaaattg atcagccaag catttggaca
    10081 taaacgcttc atgtcttcaa cgattgccaa aaccactgga acggtacgaa aagctttgaa
    10141 tatcccgcca gcaccatttg tttcttgccc aatcatccca taataactag ggatccgctc
    10201 gtctttgatt cgtgcatcta attgaccgac acgaaactga gtcgtaacaa aatccgcatc
    10261 tttcaatgct tcttcccgat ccagagttgc atagattttg acttcatacg gagaagcatc
    10321 ccacatacgc tgagacatct gactgacgat ctcaagcttt tcttttcctt cttctacatc
    10381 tacgagccag atttctcgta tcggtaattc ctcatatctt ttgataaatc cttccatcaa
    10441 ctcgggggtg taactgctgc ctccaccaat cgtagcgatt ttgattcctt ttgccatgtt
    10501 ctcactcctt tactttttac acttttatta tcttttttgt aaacaaaaca aacaatagaa
    10561 attcgtttct ttccagtaac atttacaaaa gttagtgttt tctactggat ttttgtaaat
    10621 atcttgtcaa aaaatgtctt tattaagcta tataacataa atccgtttac aaatcatttt
    10681 caaaaaagtt ataatttacg taaaaaaaca agaagaaggt atctacatgt tattacgtga
    10741 aaaaatcaaa gaaatctcct tttctccttc agaaaatgaa gtgattcagt ttctcttaag
    10801 cgattctcca gcacttggcg aaatgaccat ccaagagatt gctgagcgat gttatgtcca
    10861 cccgtccaca ctcatccgtg tagcaaaaaa aatgggtttt gatggctggg tcagttttcg
    10921 taatgctttt ttagctgaaa cagcctatct tgaagggaat tttcaagacg tagatgcgaa
    10981 tcttcctttt tccaccacag acggtattat gacgatcgca aataaaatgg cttctttaga
    11041 acagatgacg attcaagata cactttctct cattcatcat gaccaactgc aaaaagccaa
    11101 acaactaatg cttcaagcaa aaaaaatcgt gattttcgcc agtaactcga atactttgat
    11161 ttctcaagat ttcatgctga aaatgaagcg catcaaaatg gatgtcacga ttgtcaccac
    11221 ttttggtgaa tcgttctatg aagcgtacaa ttgcacgagt gatatgtgcg ggattctgat
    11281 ttcttatacg ggtgaaaaca atgtgatcct tcgtaccgca gaaatattga aacaaaatca
    11341 tgttccaatt ttagctttga ccagtatcgg tgattcaacg ctatcttcaa tgagtacagc
    11401 cgctttgcga cttacgacac gcgaacgact gtattcgaaa attggtaatt ttacgatcaa
    11461 tacatcgatt tgttatcttc tagacgtgtt atatagttgt gtttttgcag aaaattatca
    11521 acagaattta gtccatctaa tttctgtagg acaaaaattt gataaacgcc ctatcagctc
    11581 agccatcatg aaagagcctt tcaattttga aaataaagaa gtggactaaa aagaataatt
    11641 aagcctagtc cattctttca cctaaccgtt caggtcagtg cggtgataag tgcatcgtct
    11701 ggcccgaccg tctccccttg attttccaaa atatccaaat aatccgtgta attggtgacg
    11761 atcacaggtg tttctaaact gtatccttcc tcttcgattt tctttttgtc gaagcggagc
    11821 aacagatcgc cttgtttcac tctttctcct tgctgcacca agctttcaaa atatttccct
    11881 tccaactgaa cagtatccaa tccgacatga atcagcaatt ccagacctgc ttcagaaacc
    11941 attccgattg catgtttcgt cgggaacagg gtcatgactg ttccgtcaaa aggggcacgt
    12001 acttctcctt tttcagggta gatcaagatt cctcgaccta gtgttccttg tgcaaaagct
    12061 gcatcctttg ctgcattcag tggagcgatt tgtccttgta tcggtgtttt tacaatttct
    12121 ttacctgatc ttttctgtct atcatctgtt tcacctgctt cctccttcca aaagaaaaaa
    12181 gtcagcgcaa aggataccac catcgcaatg gcgattccaa tcaatacatt aatcacatcc
    12241 gttaaatcac catctggtct aatatagttg ggaattgtaa agatgcctaa tccacctgga
    12301 ttccaagaag ttaaattcat taatccacta tacgcgcccc ctaacgcacc accgacacag
    12361 gtaaaaataa atattttttt acgtggcaat gtaaacccat aaatagctgg ctctgtaatc
    12421 cccacaatac cagaaacaat ggctggtatt gcgattgctt tgtcttttgg gtttctaagc
    12481 ttaaagtaga gtgcagtaag tgctgcagtt tgtccaaaag acactactgc aacaggtgtc
    12541 atgatatttt gccaaccatt cacagaaaat tgcatcaatc ccattggtac gattgcccaa
    12601 tggagaccaa acatcactag aatttgccag aagaagccta cgattgctcc ataaataact
    12661 ggcgaaagtg ctaaaagtgt ttcaaatcca gccattaaaa tatcagttgc gaatgtgaat
    12721 gctggtccaa cgagtaaaaa acctaagggc attgtaataa gaacggtgaa gaatggaacg
    12781 aagaaattcg cgatcatctc aggtactaac tttttaattg gtttctgaat ttggctagct
    12841 aacaacacaa taaagataat gggaatgaca gaagaagcgt agttacgcgc aatccaggga
    12901 atgccgaata ctgtttgata aataggtgct tcaaaaatag tgccagaaaa tatggtagtc
    12961 agtggtgctt ctgctgcacc actcaaagct tccagcgaca agctaggatt cattaatgct
    13021 gccccaagca acatccctac gaattcattt actttaaatt tacgggcagc tgttaaaccg
    13081 ataataacag gcataaattg gaatacagaa tcaccgatat tatcaaacat aacaaatgtg
    13141 ccagaaccgc ggtcaaatgc gcctaagaag acaagtaatg ctgtcagtcc acgaatcatc
    13201 ccaccagcag ctagaatagc aaggaatggc tggaaacagc ccgatattcc atcaataagc
    13261 atatcaaacg gtctcatttt ttctgttgaa acagcctcat caaaagtaag gtttcctgct
    13321 tttataacct cttcaaacac gactggcaca tgattcccga tgacaacttg gtactgtcct
    13381 cccgctcgca ttaccgtcac aacaccgtcc atatttttca agatctcatc atttgctttt
    13441 tcttcgtttt ttaatttgaa acgaagcctt gtgatacaat gtgtcaaacc attgatattt
    13501 tccttcccgc cgacattttc tacaattttg ctcgctaatt cttgatattt tcccatttct
    13561 attccctcca atttattagt ggaaggttta gccaactgaa tgtcactacc cttatttcat
    13621 gcgttcttgt taatttatcc tccttttttt atttatttac aacgattctc atgcactaaa
    13681 cgcgcaatat ggatcgttaa atacattttt tcgtccgtgg acatctggta gttgaatatc
    13741 tcggctaaaa agtctgctat tttctcaacg caaccaaacg tatccttata ttttttttga
    13801 ataacctggt acaattcctc agaaacttct cccacttctg atttttctcc atctaggacg
    13861 cgtgtcagaa aaaaacgtag gtgtgtgacg aatcgaaaaa aatggactga atcttcatca
    13921 aatgtaatct tatagtaata gcggacgata tttagaatat cctgtattgt tgtagtaaac
    13981 aggtaggtat tgtcttgttg gctttgaatg ccttgtgcat tgactaaatg caatgcgata
    14041 aatccggctt catcttctga taacttgatg tggtatttat ccgagatttt cttcactgtt
    14101 tttttcccga tggcatactc tttaggaaat agttgtttga tatcatacag caatacattt
    14161 ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc cgtcaatgca
    14221 atatacaccg aatcattcaa ctggacttgt agagcctctt ttgtatattc aatgatttca
    14281 caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa ctgttgtgaa
    14341 agtccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat cgggtcaccc
    14401 actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt tctttcatcc
    14461 ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgtttta ctcctttcca
    14521 tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc catggaacca
    14581 tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg tatcgttttc
    14641 tttatattat cgaaagcaag cacttttgtc aacgctttct tagcggctat tgggcacttc
    14701 tgactagctg tcgtgatttt ttttaaatct ggtaattacg acactaacgc tcacagatga
    14761 agaatctggg aagctctgtt ttattttcac tctagcgctt aggtcaaaat gctactatgc
    14821 tactgtccca acttctaata tacggtgttc cagtaccagc ttatcggtga taagccaaat
    14881 ttatgcaaaa atctggaaag ccttttttac aatttttttc ttatcaccta aagctgaacg
    14941 gagccctgtc acaaccactt tttacacacc actgctttgc agtttgtaca tatctgcata
    15001 caaaccattt tgcgctagaa gtgtttcatg attcccacgt tctactatat gacctttgtc
    15061 taaaactaaa atcaattctg catcccgaat cgtggacagc cgatgggcaa tcgcaatcgt
    15121 tgtccgtcct tgtcgcattt ttgtcaatcc ttcttggatc aatccttctg tttcagtatc
    15181 gatatttgct gtcgcttcat ccaatactag tattttagga tctgtcacga ttgttcgcgc
    15241 aaaagtgatc agctgtcgtt gtccgctaga atagctggca ccgtgttcga tcacttttga
    15301 gtgatatcct tttggtaatt catcgatgaa cttgtttgct tgaacaaact cagctgcatg
    15361 gcgaatctgt tcgtctgtga tagttggatt catcatccgg atatttcctg cgatatctcc
    15421 ataaaacaag aaggcatctt gcaagaccaa tcccattttc ttacgtaact cttccattgg
    15481 atattctcga atgtcatgat catcaatcaa gatctggcct tcataaaatt cgtaaaaccg
    15541 catcaatacg ttgatgatcg agctttttcc gcttccggtg tgccccacta atgcaacggt
    15601 ctctcccgga ttagcgacaa aagagatatt gttcaataca ttgtgctctc catcataaga
    15661 aaagcttaca ttgcgaaact cgatcttacc tgaagtaatc gcttgatctg ctccaggatt
    15721 ttgttgcggt gcatattctt ctgtatccat gattttcaaa atacgactac cggcaaccat
    15781 tccatctgta aaggtgctca aaaagtccat catttgagac attggattaa aaaatccctg
    15841 gacataggtc gtaaatgcat agatcagtcc aacttctacg ggagaatgca gcgcatcgat
    15901 cccaaacaaa gtcaacgcca acgcaatcgc caatgcatat agaaaattga tgatcggact
    15961 taacagaatc gaatttgttt tgatcatcgc atagcgcgtg tctaaatact cgttgttcac
    16021 ttgttcaaat tctttagcta aacgagcttc ttggcgaaac tgctggataa tacgcatgcc
    16081 ggaaatcgat tcattcaatt tcgtgttcag ctgacttaat ttttcccgca tattccggta
    16141 aatacgagaa ctgaatttct ggtaatacca gatgacgatt cctaaaatcg gcaagaacac
    16201 caaagtccac aaggcaattt ctccattgat ttgaaacatg gcaatgaagg aagaaacgac
    16261 tgcaaagatc ccagtcaata ccatcaaaaa gacataccaa aattcaaata gcgtttctgt
    16321 gtcatttgtc actcgagaaa cagtcgatcc tgccggtgtc tgatcaaaat aacgcatacc
    16381 tagtgtatgc agcttttcaa ataatcttac ccgaacatac tgataggtct tcaatgaagc
    16441 catggaatac aaataccatt ggaaaaacca aatgatactt ttgactatga ctccaaacaa
    16501 gtacatacca gcaaaaaaca aaataacttg tgtcgtagcc gactgtttag ccagatgatc
    16561 atccataaaa gtttggatga tccttggcaa taggacattg atcacagaca gggcaaacgc
    16621 aaataagata gcaacgatga acgttcttct aaaaggtttg gcgaacttca tcagtcgttt
    16681 gatgatcttc ttctgttctt taagtggaat cgattttgtc cattccgatt gataattttc
    16741 atccatcagg cctcgcctcc ttctatcttc gcttctaatt tctgtttctc ccacattcgt
    16801 tcataccagc cgcctaatgc aagcagctca tgatgggttc ctcgttcaat gattttccct
    16861 tgatcgacaa caatgatttc ttttgcatgc atcacgctgc ttaaacggtg agctgcaatg
    16921 atggttgttt tctcttttct tatttctttt aaattatgca agatagcttc ttctgttttg
    16981 gcatctactg cagaaagtgc atcatctaag atcagcaatt caggattttt gatcaatgct
    17041 cgagcaatcg aaatcctttg tttctgtccg ccagataaag agactccacg ttctccaact
    17101 aaggtatcat aaccttttgg taattccttg atttcattgt tgataaatgc taaagaagcc
    17161 gcatgctcta cttcttcttc tgaaagtttt ggattagaaa aacggatatt atctcgaatg
    17221 gtcatcgaaa acaagaagtg atcttgaggc acatagccga ttgatcctaa taaagcattc
    17281 aatgaatatt ctttgatcga acatccgcca aattgaattt ttccttgata atcatcgtat
    17341 tcacgcatca gtaatttgat gattgtcgtt tttcccgcac cagttttccc aacaatacct
    17401 aatgtttctc cttcttggat cgcaaatttg atttgttcta atgctggtga ttcttctttc
    17461 ggatagttaa aagaggaaat agctacattc agctcgcctt ttgccggagt ctgcactgca
    17521 cccagttgtt ccacgatatg cgtcttttca tgcaaaagtt cattgacgcg atcataagaa
    17581 gcatttcctc tttccaatac gttgaacaat cgaccaatcg caaacatcgg ccaaacaagc
    17641 ataccgatat agctaataaa tgagaccatc tgcccgattg agatcgtgcc ttccaagaca
    17701 tactttccac ctaaaataat tgttgcaaca taagacagac cgataatcaa ggtaatgaat
    17761 ggatcaaata aggagtctaa aaaattgact cgtttgtttt taataattgc atcttcaatc
    17821 ttttcttcaa aatccgcgag atcttctttt tcttgtccaa atgttttaat gactttgatc
    17881 ccagtaatac tttcttgtgt cttatcattg atactagaaa aagcttcttg cgaatcacga
    17941 aaagcatcgt ggagttttga ccccagcact cgtgaagtaa cggctagtaa aggcagagga
    18001 atcagcgcaa tcagtgttaa gcgccaatca ataaacaaaa tcatcgcaat gatcgttgtt
    18061 ccgcccgtga tcactgaatc tgcaaaggtc aagataccag ctcctgctac attttggatt
    18121 gcattcaaat cattcgttgc atgagccatc aaatctccag tccgatattt ttggtaaaag
    18181 agattgtcca tttctgtaaa atgtttgaat agctgctgcc gaagttcttt ctccaaccga
    18241 gctgcacttc cccaaatatt cgtccgccag atataacgaa aaagatattg taagagagct
    18301 gcaaggatca ataataagat ccaccaggaa atgatcttta ttgataaatt tttatcggcg
    18361 atctcatcaa tgatgatccc aatcactttt ggaggagcta actgtaccaa ggccactgcg
    18421 aatagggaga aaatcccaat taaataatgt ttcttttcct gtcgaaaaaa ccaacctaat
    18481 tttctaaaaa tcgacatttt actcctcctt tgtctttatc aagagcatag gattgtctgt
    18541 gcttgtcttt tttgctgaat agtctgaata gtctaacaaa ttacgaaata aaaaacggac
    18601 tgaaaaaatc agcccgcaaa aagtctttgg gtcagaacct attttttccc ttggttcttc
    18661 atttgctgca tcatctggcg aattttcttc tcagatggtt tttgccccat tgacatcatc
    18721 attgatcgca acatgtcctc attaacggga ggattttttt ctaaatagtc tttcatatat
    18781 ttacgcgcta ggaaaaagcc acctactgcg ccgattagca aagcaataat agcaataagt
    18841 acgacaatac ctgttgatac catataaatc tctcctttct atttccgaat tgctctcaat
    18901 gatttatttt actaaagttt attgaaaaaa gaaaggcgta ttctttagtt tcatgcaaaa
    18961 aatagtggtt tttttagaga agaacaaaat ttctttcatt aaccagcaag acacgccacg
    19021 aaaaaaaatt aggcatacca aatctttttc tttaattttt tctttctgag gtgtagaatg
    19081 taatcgtaga taaataggaa aggtggtttt ttcttgaata atcaatccaa caaacaattt
    19141 cataagcatc gacttattga acatacaaat ggtttatctt tagctgaagt aaatggaaca
    19201 gtggatgtac ccaagggaaa aagttttttt aaaacacttc ttgcttactc tggaccaggt
    19261 gcattagtag cagttggtta tatggatcca ggtaactggt ccacttcgat cactggtgga
    19321 caaaatttcc aatatttatt gatgtcagtt attttgatgt ctagtctgat tgctatgctt
    19381 ttacaatata tggcagcaaa attaggaatc gtgacccaaa tggatttggc ccaagcaatc
    19441 cgagcacgga caagtaagac attgggtgtc attttatgga tcttgactga attagccatt
    19501 atggcaacag acatcgcaga agtcattggt gcagcaatcg ccctctacct gctttttgat
    19561 attccattgg taattgccgt atttatcact gtgttagatg ttttcgtcct tttattgctg
    19621 acaaaagtcg ggtttcgtaa aatcgaagct ttggttgtct gcttgatttt cgtcatttta
    19681 ttcgttttcg tctatcaagt tggcttgtct aaccctgatt ggcacagttt gtttgccgga
    19741 tttatcccta acagtcacac attctcagac tcaccggtca tcggcggcga aactccgctg
    19801 actggagctt tagggattat tggtgcaaca gtcatgccgc ataacttata tcttcattca
    19861 gcagtttctc aaactcggaa gatcgatcac caagacgaag aagatgtagc acaagcggta
    19921 cgcttttcta cttgggattc taatatccag ttgtcaatgg cttttctagt caatgcactg
    19981 ctattgatca tgggtgtggc tgtattcaaa accggttctg tccaagatcc ttcctttttt
    20041 ggtctgtatg aagctttatc tgatccatca gctatgagca atggtatctt gattgctgta
    20101 gcaaaatctg gtatcttgtc tactttattt gctgtagcac ttcttgcttc tggccagaac
    20161 tctactatta ctggtacgtt gaccggtcaa gtcatcatgg aaggcttcat ccatatgcgt
    20221 atcccaattt ggctaagaag actagtcaca cgattgcttt ctgtgattcc tgtattgatt
    20281 tgtgttttgt ttacaagtac aaaaggaacg atcgaagaac ataccgcttt gaataacttg
    20341 atgaatgaat cccaagtttt tctggctttt gcattgccat tctcgatgat tcctttactg
    20401 atgatgacca atagccaagc ggagatgggg agccgattca aaaattcatt tgttgtaaaa
    20461 ctacttggct ggttctctgt gattagtctg acttacttga atttacgagg attaccaggt
    20521 caaatcgaaa gtttcttcgg cgagcaagcc acctcagctc aactagcatt ggctgatcaa
    20581 atcgcttatg taatcattgt tcttgtcctt ctcctgttgg catggacgat tggtgattta
    20641 tataaaggaa acaaaaaata tgaacagcgt ttgcaggaaa cgatagaaga aatcgagtag
    20701 aaactgattc ttttacacaa aggaggttgt gtcagaagtg ggcagcttca agaaataaga
    20761 aggaatttaa gaaaattgct tttcaaattt ttgtgaaatt tcggcttatt tccgaagaag
    20821 ttacttctgg aacaccgtgt at
//
LOCUS       DAZUMI010000047        20395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000047 DAZUMI010000000
VERSION     DAZUMI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.047"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACG81C_001452"
     CDS             complement(383..1849)
                     /locus_tag="ACG81C_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5064620.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /locus_tag="ACG81C_001453"
     CDS             complement(2140..4224)
                     /locus_tag="ACG81C_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5064621.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4366..4488)
                     /locus_tag="ACG81C_001454"
     CDS             complement(4366..4488)
                     /locus_tag="ACG81C_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064622.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4749..5108)
                     /locus_tag="ACG81C_001455"
     CDS             complement(4749..5108)
                     /locus_tag="ACG81C_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5064623.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5138..5479)
                     /locus_tag="ACG81C_001456"
     CDS             complement(5138..5479)
                     /locus_tag="ACG81C_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064624.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5476..6150)
                     /locus_tag="ACG81C_001457"
     CDS             complement(5476..6150)
                     /locus_tag="ACG81C_001457"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5064625.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG81C_001458"
     CDS             complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG81C_001458"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5064626.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8664..9854)
                     /locus_tag="ACG81C_001459"
     CDS             complement(8664..9854)
                     /locus_tag="ACG81C_001459"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5064627.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9875..10402)
                     /locus_tag="ACG81C_001460"
     CDS             complement(9875..10402)
                     /locus_tag="ACG81C_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5064628.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10449..13238)
                     /locus_tag="ACG81C_001461"
     CDS             complement(10449..13238)
                     /locus_tag="ACG81C_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5064629.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13385..13585)
                     /locus_tag="ACG81C_001462"
     CDS             complement(13385..13585)
                     /locus_tag="ACG81C_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5064630.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14038..14358)
                     /locus_tag="ACG81C_001463"
     CDS             complement(14038..14358)
                     /locus_tag="ACG81C_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064631.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14861..15979)
                     /locus_tag="ACG81C_001464"
     CDS             complement(14861..15979)
                     /locus_tag="ACG81C_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064632.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16212..16334
                     /locus_tag="ACG81C_001465"
     CDS             16212..16334
                     /locus_tag="ACG81C_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064633.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16450..17757
                     /locus_tag="ACG81C_001466"
     CDS             16450..17757
                     /locus_tag="ACG81C_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5064634.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17878..19077
                     /locus_tag="ACG81C_001467"
     CDS             17878..19077
                     /locus_tag="ACG81C_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5064635.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19104..20372)
                     /locus_tag="ACG81C_001468"
     CDS             complement(19104..20372)
                     /locus_tag="ACG81C_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5064636.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5902 a         4344 c         3559 g         6590 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     5161 ttattcttct ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt
     5221 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca
     5281 cccgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg
     5341 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     5401 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg
     5461 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     5521 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     5581 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     5641 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     5701 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     5761 aaacggctcc atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt
     5821 tcgataaacg gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct
     5881 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     5941 taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     6001 gttcatgcat tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag
     6061 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     6121 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     6181 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     6241 taaaaaatga caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta
     6301 gttttttata ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc
     6361 cagagaaatc gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac
     6421 atatatttat aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta
     6481 gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta
     6541 aacttacata tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac
     6601 tattagtttg ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa
     6661 tcagatttcc atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata
     6721 gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc
     6781 ctttatcccg tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa
     6841 cgttgctctc cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact
     6901 gttttttgaa attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata
     6961 taaacggtgt tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa
     7021 gctatttttc cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt
     7081 ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7141 gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct
     7201 ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa
     7261 catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc
     7321 acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg
     7381 tcgataccag caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga
     7441 acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga
     7501 ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt
     7561 tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga
     7621 tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt
     7681 tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg
     7741 tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat
     7801 ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat
     7861 tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa
     7921 ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga
     7981 cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa
     8041 gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca
     8101 aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc
     8161 ggatcgattt tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca
     8221 tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc
     8281 atcaagaaat ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc
     8341 acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt
     8401 tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct
     8461 tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt
     8521 aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt
     8581 tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt
     8641 atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt
     8701 ctgcgtaccg cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct
     8761 ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct
     8821 acccattgtg tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa
     8881 agatagaaag ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga
     8941 tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca
     9001 cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg
     9061 gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc
     9121 cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt
     9181 gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa
     9241 atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt
     9301 tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt
     9361 gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct
     9421 tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc
     9481 cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta
     9541 tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca
     9601 tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt
     9661 gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct
     9721 ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt
     9781 aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt
     9841 tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt
     9901 catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta
     9961 ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt
    10021 caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat
    10081 ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat
    10141 catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg
    10201 tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg
    10261 ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata
    10321 cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg
    10381 gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga
    10441 cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt
    10501 tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg
    10561 ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc
    10621 gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc
    10681 tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg
    10741 cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc
    10801 aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg
    10861 aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct
    10921 ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg
    10981 aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa
    11041 taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag
    11101 atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca
    11161 atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata
    11221 taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa
    11281 tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa
    11341 tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca
    11401 aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata
    11461 atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc
    11521 aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc
    11581 atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct
    11641 tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac
    11701 cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt
    11761 ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt
    11821 actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct
    11881 ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa
    11941 aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac
    12001 tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc
    12061 aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag
    12121 accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta
    12181 aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga
    12241 tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc
    12301 gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga
    12361 taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct
    12421 tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt
    12481 gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa
    12541 tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct
    12601 aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa
    12661 cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt
    12721 ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca
    12781 ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag
    12841 cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt
    12901 atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa
    12961 taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca
    13021 tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt
    13081 tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct
    13141 gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc
    13201 gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc
    13261 tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa
    13321 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
    13381 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
    13441 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
    13501 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
    13561 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
    13621 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
    13681 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
    13741 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
    13801 ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa
    13861 actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg
    13921 aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca
    13981 gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta
    14041 tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc
    14101 tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca
    14161 aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag
    14221 atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg
    14281 tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca
    14341 taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata
    14401 tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg
    14461 tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct
    14521 tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc
    14581 ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct
    14641 tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt
    14701 tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc
    14761 tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt
    14821 ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg
    14881 tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa
    14941 tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat
    15001 cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt
    15061 ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat
    15121 tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga
    15181 tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg
    15241 gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg
    15301 gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt
    15361 tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat
    15421 ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc
    15481 ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt
    15541 tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat
    15601 agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt
    15661 gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg
    15721 ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt
    15781 cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt
    15841 tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat
    15901 tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt
    15961 ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc
    16021 taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg
    16081 tttctacggt tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt
    16141 tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag
    16201 aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg
    16261 ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta
    16321 aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt
    16381 gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg
    16441 agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt
    16501 tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat
    16561 gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga
    16621 gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat
    16681 caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa
    16741 aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc
    16801 gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg
    16861 gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa
    16921 gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt
    16981 ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat
    17041 gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc
    17101 actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac
    17161 tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg
    17221 actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc
    17281 actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca
    17341 gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat
    17401 gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc
    17461 gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta
    17521 acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg
    17581 gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat
    17641 cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca
    17701 aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg
    17761 aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa
    17821 ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg
    17881 aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt
    17941 tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac atggacgttt
    18001 gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg
    18061 attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg
    18121 ttgaatggtc ttttattcaa tattttcctc attagttttg gtatctactt gtttgtcttg
    18181 ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac gatcccgtta
    18241 ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg gaacggtgtc
    18301 acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat actagccatt
    18361 tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc acaatggata
    18421 aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt attctacaac
    18481 tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca agacttcatc
    18541 gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct tgccaagcga
    18601 atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa tccgcctatc
    18661 ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa
    18721 caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac caattcgaca
    18781 accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc
    18841 tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct ctatgcacga
    18901 caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta cttgcctaac
    18961 gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt
    19021 tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact tttctaaaaa
    19081 aacaaggtgt gacaaaacat atgtcacacc ttgttttttt ctttcattct cttaactatt
    19141 ggcaaaaaga gtatcccttt tctgaccgct acttgctgag cagaataaat accgctgttg
    19201 cctgaacaca taaagctgat caagcagatc ataaagaagt atactgccgc ttgtgaaccg
    19261 aacaattcga tccccatcac aaagcaggca atcggtgtat ttgtcgcacc agaaaatact
    19321 ccgataaagc ccaatcctgc aagaaaagga acagaaacat ggagcaaagg cgctagtgct
    19381 gcaccaagcg ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg
    19441 cctaaagaca aaactgtgaa gaataacttc ccaataaaat catagacatg tgcctgtcca
    19501 ttaaatgatt cctgcagtaa tggaagactc aatccgagat aacgttgtgt ttgcaaaatc
    19561 aatgctgcgg ctacaactac agccccacct atcgtatttc gccaaacagg atgaggaatc
    19621 cattgtgcat atcgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa
    19681 ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc
    19741 acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcataaa atttgcaaac
    19801 agtgcagcaa agaagctagg gaagatcgct tctgttcgga ctttcccgat tgccaacact
    19861 tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg
    19921 attccactga taatcaagat ttcccgttct gatttatcca atcggaacaa acgtgctgta
    19981 ttatctgcta acgctccccc catttggact gctgttcctt cacggccaac agatcctcca
    20041 aataaatggg tagttatggt tccaaacagt gtaagtggaa taagtcgcaa cgggattttt
    20101 tcttctccac catttccttg atcgatcact aagttgtttc cacgactaga aagaccgccg
    20161 tgatacgcat agagataagc aaaaagcgca ccactaactg gtagtagata aaggagccaa
    20221 ggatgtgata gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa agcagataag
    20281 ctccccatga atataccgag aatcgctgtg atcaccagcc acttcagcat gtacactaaa
    20341 atgcgtactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat aaaaa
//
LOCUS       DAZUMI010000048        20181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000048 DAZUMI010000000
VERSION     DAZUMI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.048"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACG81C_001469"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACG81C_001469"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5064637.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACG81C_001470"
     CDS             1011..2258
                     /locus_tag="ACG81C_001470"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5064638.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACG81C_001471"
     CDS             2334..2678
                     /locus_tag="ACG81C_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064639.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACG81C_001472"
     CDS             complement(2795..3121)
                     /locus_tag="ACG81C_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064640.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="ACG81C_001473"
     CDS             complement(3494..4696)
                     /locus_tag="ACG81C_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5064641.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="ACG81C_001474"
     CDS             4813..5328
                     /locus_tag="ACG81C_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5064642.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="ACG81C_001475"
     CDS             complement(5527..5973)
                     /locus_tag="ACG81C_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5064643.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACG81C_001476"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACG81C_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5064644.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="ACG81C_001477"
     CDS             complement(7852..8481)
                     /locus_tag="ACG81C_001477"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5064645.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="ACG81C_001478"
     CDS             complement(8465..8929)
                     /locus_tag="ACG81C_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5064646.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="ACG81C_001479"
     CDS             complement(9416..10684)
                     /locus_tag="ACG81C_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5064647.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="ACG81C_001480"
     CDS             complement(10915..11652)
                     /locus_tag="ACG81C_001480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5064648.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="ACG81C_001481"
     CDS             complement(11719..12288)
                     /locus_tag="ACG81C_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5064649.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="ACG81C_001482"
     CDS             12416..13288
                     /locus_tag="ACG81C_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5064650.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="ACG81C_001483"
     CDS             13630..14502
                     /locus_tag="ACG81C_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5064651.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /gene="nagE"
                     /locus_tag="ACG81C_001484"
     CDS             14499..16457
                     /gene="nagE"
                     /locus_tag="ACG81C_001484"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5064652.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="ACG81C_001485"
     CDS             complement(16708..17559)
                     /locus_tag="ACG81C_001485"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5064653.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="ACG81C_001486"
     CDS             17682..18149
                     /locus_tag="ACG81C_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5064654.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="ACG81C_001487"
     CDS             complement(18412..18894)
                     /locus_tag="ACG81C_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5064655.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="ACG81C_001488"
     CDS             complement(18994..19857)
                     /locus_tag="ACG81C_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5064656.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3662 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgactccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DAZUMI010000049        19937 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000049 DAZUMI010000000
VERSION     DAZUMI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.049"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(156..1043)
                     /locus_tag="ACG81C_001489"
     CDS             complement(156..1043)
                     /locus_tag="ACG81C_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS5064657.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1228..2067
                     /locus_tag="ACG81C_001490"
     CDS             1228..2067
                     /locus_tag="ACG81C_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5064658.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2263..3237
                     /gene="sgrA"
                     /locus_tag="ACG81C_001491"
     CDS             2263..3237
                     /gene="sgrA"
                     /locus_tag="ACG81C_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5064659.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3535..3891
                     /locus_tag="ACG81C_001492"
     CDS             3535..3891
                     /locus_tag="ACG81C_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064660.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3936..5051
                     /locus_tag="ACG81C_001493"
     CDS             3936..5051
                     /locus_tag="ACG81C_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064661.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5077..5517)
                     /locus_tag="ACG81C_001494"
     CDS             complement(5077..5517)
                     /locus_tag="ACG81C_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5064662.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5677..6183
                     /locus_tag="ACG81C_001495"
     CDS             5677..6183
                     /locus_tag="ACG81C_001495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5064663.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6351..8264
                     /locus_tag="ACG81C_001496"
     CDS             6351..8264
                     /locus_tag="ACG81C_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5064664.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8269..9270
                     /locus_tag="ACG81C_001497"
     CDS             8269..9270
                     /locus_tag="ACG81C_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5064665.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9472..9678)
                     /locus_tag="ACG81C_001498"
     CDS             complement(9472..9678)
                     /locus_tag="ACG81C_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5064666.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10324..11172)
                     /locus_tag="ACG81C_001499"
     CDS             complement(10324..11172)
                     /locus_tag="ACG81C_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5064667.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11336..12238
                     /gene="gnd"
                     /locus_tag="ACG81C_001500"
     CDS             11336..12238
                     /gene="gnd"
                     /locus_tag="ACG81C_001500"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5064668.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12240..13241
                     /locus_tag="ACG81C_001501"
     CDS             12240..13241
                     /locus_tag="ACG81C_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5064669.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13238..13522
                     /locus_tag="ACG81C_001502"
     CDS             13238..13522
                     /locus_tag="ACG81C_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5064670.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13555..14352
                     /locus_tag="ACG81C_001503"
     CDS             13555..14352
                     /locus_tag="ACG81C_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5064671.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14366..15199
                     /locus_tag="ACG81C_001504"
     CDS             14366..15199
                     /locus_tag="ACG81C_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5064672.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15217..16074
                     /locus_tag="ACG81C_001505"
     CDS             15217..16074
                     /locus_tag="ACG81C_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5064673.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16096..16605
                     /locus_tag="ACG81C_001506"
     CDS             16096..16605
                     /locus_tag="ACG81C_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5064674.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16642..17043
                     /locus_tag="ACG81C_001507"
     CDS             16642..17043
                     /locus_tag="ACG81C_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064675.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17155..17508
                     /locus_tag="ACG81C_001508"
     CDS             17155..17508
                     /locus_tag="ACG81C_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064676.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17542..17985
                     /locus_tag="ACG81C_001509"
     CDS             17542..17985
                     /locus_tag="ACG81C_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5064677.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            18002..19087
                     /gene="uxuA"
                     /locus_tag="ACG81C_001510"
     CDS             18002..19087
                     /gene="uxuA"
                     /locus_tag="ACG81C_001510"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5064678.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19080..19793
                     /locus_tag="ACG81C_001511"
     CDS             19080..19793
                     /locus_tag="ACG81C_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5064679.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         6811 a         3075 c         4172 g         5879 t
ORIGIN      
        1 cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa ggccccgatg
       61 aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa atcacttctc
      121 aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt ttttacataa
      181 ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt atccatgaat
      241 tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt gtatttgtct
      301 ttattagaac ctttatcctg tttttttacc atttttacta gataatagct ttgctgataa
      361 gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag tttaaatgct
      421 gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat ttttccatca
      481 gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa attttctcct
      541 tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag aatgatctgt
      601 gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc tattaatttt
      661 acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt cttatatgtc
      721 tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag ttttttgatt
      781 tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta tacatcatca
      841 aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt aacttttggg
      901 ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat cgtttcattg
      961 gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat aattagtagt
     1021 tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca gaagaattag
     1081 ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt aaaaatctat
     1141 tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa aagggttgtt
     1201 aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat tggatctgtt
     1261 ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact tgcggatatg
     1321 tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca tttagctgtt
     1381 tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga agattatggt
     1441 gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt tggaattgga
     1501 gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt agattcagga
     1561 aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt gggggcatca
     1621 gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg tccgttaata
     1681 gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc taacatgaca
     1741 tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact tatctacagt
     1801 acaggaagtt tatttacggc tattggtgct catattactt ggaattggac acaaggagct
     1861 attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac tctgatccaa
     1921 tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat atcaactaca
     1981 ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac aaagcggatg
     2041 aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat atcggtcttg
     2101 cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg aatacacgac
     2161 atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat tttatataat
     2221 tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa aacagcgacc
     2281 gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat cagtacttct
     2341 gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg gattgtttat
     2401 gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt gaatgctagt
     2461 gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga aaaagttatt
     2521 ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac aataatgatt
     2581 tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt gattgttttt
     2641 tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac agttgttgga
     2701 ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac gagcggtgaa
     2761 agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag cgaaagcagt
     2821 acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag cagtacggag
     2881 agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac aggaagcacg
     2941 agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc tatcatacca
     3001 actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa acagcctgat
     3061 tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa tagaaaaagc
     3121 gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat gggtgtgtta
     3181 ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt gaaataaaga
     3241 ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa tatttcattc
     3301 gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt gtttactaac
     3361 atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa gaataattga
     3421 caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta tttgtacgtt
     3481 attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg tactttgttt
     3541 gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta ttgttatgaa
     3601 agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta caccctggct
     3661 aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc agaatataaa
     3721 ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca gtttttactg
     3781 agtaaagttt attctatact tttagaggga acgattggtt atattttact ggattcactt
     3841 tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata agtttgcgta
     3901 cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat ttatcttgca
     3961 gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac ttttttcact
     4021 tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt ataaaaaata
     4081 aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt atagatagaa
     4141 agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc cactcgatta
     4201 cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa gaagagttta
     4261 cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat cacatcactc
     4321 caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat ttggaagaag
     4381 atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa tcgtctattg
     4441 tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa aattatctat
     4501 atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa aagaaggtgg
     4561 atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca gtgatgtatc
     4621 caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt aatacttatt
     4681 atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat atttttataa
     4741 aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca caggaattat
     4801 gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt gcatccgctg
     4861 atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca tatgatcaaa
     4921 cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg gcgcgtctga
     4981 aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa aaattggatt
     5041 tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa aaagattatt
     5101 ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt gctttctttt
     5161 agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa gttttgcaga
     5221 ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct caatatatag
     5281 ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa tagaatgagt
     5341 agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa attcattttt
     5401 ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga cgataagcca
     5461 acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa tcatcataca
     5521 ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg aaaagtgcca
     5581 tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta gataagaaat
     5641 actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc cgacgcaata
     5701 gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca cgaattagaa
     5761 acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg gaaaccagtt
     5821 attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg ggagctattg
     5881 tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc gaattcattt
     5941 tataagacta atttgagtgt tttattaact gaattggact gtcattcgat cgaattatgt
     6001 ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg attaggatac
     6061 cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat gaaagcaaag
     6121 gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt tttggagcaa
     6181 taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat tctgctttat
     6241 tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt tattttttgt
     6301 ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga gtgaatagct
     6361 tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta agtacaagcg
     6421 tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct cgggaggtag
     6481 aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca caaacagaaa
     6541 caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa acgaccgcta
     6601 ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac gacgaatcct
     6661 cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc ggtaaaggtg
     6721 aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca gaaatactta
     6781 atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact gagcttgctg
     6841 ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact tatctattgt
     6901 acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt acagatgatg
     6961 ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt catcaaactg
     7021 tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc gatcaaggtt
     7081 cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac gggatcgtta
     7141 cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt gattccagag
     7201 cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca gatgacgtcc
     7261 tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta ctttatggtg
     7321 gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct gtcaatgtca
     7381 aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt tttacgtaca
     7441 gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac atcattgggg
     7501 atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt gatcaggaga
     7561 tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca ttgcctaaat
     7621 tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa aaattatatg
     7681 agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat ggaaaggcac
     7741 aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa gtagtgttcc
     7801 atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc aaagaccagg
     7861 acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa aagacattcg
     7921 aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat gagacgatac
     7981 caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag caagatccga
     8041 cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca agtgatgata
     8101 cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa aaaagaatgt
     8161 tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga tttttatctg
     8221 ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat gaaatggttc
     8281 aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac tgtatttttg
     8341 attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt tgacacaagt
     8401 gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga acaaatcgat
     8461 ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga tatctacaca
     8521 ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg cgcatatgta
     8581 ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga cacgcagcaa
     8641 atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac accagttatc
     8701 caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc agattggtcg
     8761 aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata tttgttagca
     8821 cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga gaacaagcag
     8881 attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac tcaagtaagt
     8941 gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga taataaggac
     9001 tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga tcgttggacg
     9061 caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat ggaagaagta
     9121 acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc ggagaaaaat
     9181 gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca agaaacatat
     9241 caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag gttgtaatct
     9301 gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca tggtttatga
     9361 atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta ttcgaaattt
     9421 gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa gtcagatttg
     9481 ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa gaagggacaa
     9541 aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt tattgatata
     9601 aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa aaagcaaaga
     9661 aatatggata gctttcataa gcagcacctc cttttttata attgtatgat aaatagatta
     9721 agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc tagcgaactt
     9781 gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag gtacaaagtt
     9841 taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa aagatttagg
     9901 atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt attttacata
     9961 acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag gccaaaagcc
    10021 agtgttatca aggaaatact taattcacca aaattttttt taatttttta gaaaataaga
    10081 aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact aactttgtta
    10141 tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga tgcgatgaca
    10201 tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca
    10261 ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt ttctgttatg
    10321 gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac gacgtgattc
    10381 tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta gttgagaaat
    10441 acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa taaaaacgac
    10501 atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg tttttgctcc
    10561 agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg tgtgagaaat
    10621 caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa tatggtaatc
    10681 cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt cagttgctac
    10741 aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga taatagtagc
    10801 cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag ttgaaatact
    10861 agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa tattttcgtg
    10921 gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa aatctttaaa
    10981 tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc
    11041 acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta aaatataatc
    11101 ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt gtagtcttat
    11161 ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt ccgttctcat
    11221 taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc
    11281 tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg ctatcatgga
    11341 aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact taaaagagca
    11401 ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg caagagaaaa
    11461 tgacattcct gcttatcaag agatctctga atttctagct acttttgagc agaaaaaaat
    11521 catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa aactttttcc
    11581 aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata aagattcaat
    11641 ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg ggacttctgg
    11701 aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt
    11761 tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg
    11821 tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat
    11881 gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga
    11941 acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat
    12001 ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc
    12061 aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat
    12121 tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt
    12181 agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa
    12241 tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc
    12301 acttggaaag attaaagtca gttggagacg ttcatttctt taatgttgat caagagattc
    12361 caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc
    12421 cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg
    12481 gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta
    12541 tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt
    12601 ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc
    12661 gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa
    12721 ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt
    12781 atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc
    12841 aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact
    12901 atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata
    12961 gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga
    13021 tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt
    13081 tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag
    13141 aagcaatgtg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg
    13201 aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg
    13261 tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt
    13321 ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc
    13381 atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta
    13441 tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa
    13501 aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt
    13561 tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt
    13621 gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca
    13681 gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc
    13741 cctgttccat atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag
    13801 ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact
    13861 gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca
    13921 caaagaaaac tgtcatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt
    13981 ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc tatgcatgac
    14041 gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat
    14101 gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct
    14161 ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat
    14221 aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt
    14281 cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc
    14341 agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat
    14401 tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt
    14461 acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca
    14521 agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg
    14581 atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg
    14641 ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta
    14701 cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt
    14761 agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc
    14821 atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc
    14881 tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg
    14941 agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct
    15001 ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat
    15061 ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg
    15121 gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga
    15181 ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa
    15241 acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat
    15301 tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga
    15361 atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa
    15421 cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta
    15481 atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg
    15541 caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga
    15601 gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca
    15661 caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt
    15721 ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta
    15781 aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg
    15841 atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc
    15901 ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta
    15961 gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca
    16021 ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa
    16081 gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat
    16141 gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac
    16201 gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca
    16261 ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa
    16321 tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta
    16381 aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat
    16441 acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa
    16501 gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc atctgcacct
    16561 aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa
    16621 aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt
    16681 tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca
    16741 ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta
    16801 gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt
    16861 acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac
    16921 caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt
    16981 tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta
    17041 taaacaggta tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct
    17101 cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta
    17161 gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt
    17221 tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt
    17281 tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta tttttcaaat
    17341 aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt
    17401 agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa
    17461 acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca
    17521 aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc
    17581 gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg
    17641 tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact
    17701 atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac
    17761 aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc
    17821 atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca
    17881 ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg
    17941 catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa
    18001 gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt
    18061 tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga
    18121 tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga
    18181 actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg
    18241 agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg
    18301 attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga
    18361 aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc
    18421 aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga
    18481 agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga
    18541 agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc
    18601 agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag
    18661 agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa
    18721 tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat
    18781 catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg
    18841 agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca
    18901 tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag
    18961 aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact
    19021 cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta
    19081 tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa actggacgtt
    19141 tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag
    19201 tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt
    19261 acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa
    19321 tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg
    19381 caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa
    19441 tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg
    19501 taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc
    19561 ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag
    19621 cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga
    19681 agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt
    19741 atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag
    19801 gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag
    19861 aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac
    19921 aaaacgcatc tactgtt
//
LOCUS       DAZUMI010000050        19507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000050 DAZUMI010000000
VERSION     DAZUMI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.050"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG81C_001512"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG81C_001512"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5064680.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG81C_001513"
     CDS             complement(1173..1868)
                     /locus_tag="ACG81C_001513"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5064681.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG81C_001514"
     CDS             complement(1881..2597)
                     /locus_tag="ACG81C_001514"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5064682.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG81C_001515"
     CDS             2927..3532
                     /locus_tag="ACG81C_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5064683.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG81C_001516"
     CDS             3857..4180
                     /locus_tag="ACG81C_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5064684.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG81C_001517"
     CDS             4362..5732
                     /locus_tag="ACG81C_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5064685.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG81C_001518"
     CDS             complement(5862..7163)
                     /locus_tag="ACG81C_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5064686.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG81C_001519"
     CDS             complement(7393..9384)
                     /locus_tag="ACG81C_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5064687.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG81C_001520"
     CDS             complement(9528..9794)
                     /locus_tag="ACG81C_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5064688.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG81C_001521"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG81C_001521"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5064689.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG81C_001522"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG81C_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5064690.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG81C_001523"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG81C_001523"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5064691.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG81C_001524"
     CDS             complement(13954..14475)
                     /locus_tag="ACG81C_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5064692.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG81C_001525"
     CDS             complement(14478..15320)
                     /locus_tag="ACG81C_001525"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5064693.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG81C_001526"
     CDS             complement(15313..16038)
                     /locus_tag="ACG81C_001526"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5064694.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG81C_001527"
     CDS             16214..16510
                     /locus_tag="ACG81C_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064695.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACG81C_001528"
     CDS             17078..18670
                     /locus_tag="ACG81C_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5064696.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACG81C_001529"
     CDS             18683..19423
                     /locus_tag="ACG81C_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5064697.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAZUMI010000051        19140 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000051 DAZUMI010000000
VERSION     DAZUMI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.051"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..294)
                     /locus_tag="ACG81C_001530"
     CDS             complement(70..294)
                     /locus_tag="ACG81C_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064698.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(314..736)
                     /locus_tag="ACG81C_001531"
     CDS             complement(314..736)
                     /locus_tag="ACG81C_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5064699.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(878..1588)
                     /locus_tag="ACG81C_001532"
     CDS             complement(878..1588)
                     /locus_tag="ACG81C_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5064700.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1606..2103)
                     /locus_tag="ACG81C_001533"
     CDS             complement(1606..2103)
                     /locus_tag="ACG81C_001533"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064701.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2140..2592)
                     /locus_tag="ACG81C_001534"
     CDS             complement(2140..2592)
                     /locus_tag="ACG81C_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5064702.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2727..3332)
                     /locus_tag="ACG81C_001535"
     CDS             complement(2727..3332)
                     /locus_tag="ACG81C_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5064703.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3782..4402)
                     /locus_tag="ACG81C_001536"
     CDS             complement(3782..4402)
                     /locus_tag="ACG81C_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5064704.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4404..5273)
                     /locus_tag="ACG81C_001537"
     CDS             complement(4404..5273)
                     /locus_tag="ACG81C_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5064705.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5296..5703)
                     /locus_tag="ACG81C_001538"
     CDS             complement(5296..5703)
                     /locus_tag="ACG81C_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5064706.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5722..7011)
                     /locus_tag="ACG81C_001539"
     CDS             complement(5722..7011)
                     /locus_tag="ACG81C_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5064707.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(7038..7340)
                     /locus_tag="ACG81C_001540"
     CDS             complement(7038..7340)
                     /locus_tag="ACG81C_001540"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5064708.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(7365..9458)
                     /locus_tag="ACG81C_001541"
     CDS             complement(7365..9458)
                     /locus_tag="ACG81C_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5064709.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9947..10711)
                     /locus_tag="ACG81C_001542"
     CDS             complement(9947..10711)
                     /locus_tag="ACG81C_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5064710.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(10822..11691)
                     /gene="mdcB"
                     /locus_tag="ACG81C_001543"
     CDS             complement(10822..11691)
                     /gene="mdcB"
                     /locus_tag="ACG81C_001543"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS5064711.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(11716..12792)
                     /locus_tag="ACG81C_001544"
     CDS             complement(11716..12792)
                     /locus_tag="ACG81C_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5064712.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(12816..13409)
                     /locus_tag="ACG81C_001545"
     CDS             complement(12816..13409)
                     /locus_tag="ACG81C_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS5064713.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(13413..15089)
                     /gene="mdcD"
                     /locus_tag="ACG81C_001546"
     CDS             complement(13413..15089)
                     /gene="mdcD"
                     /locus_tag="ACG81C_001546"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS5064714.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(15079..15378)
                     /locus_tag="ACG81C_001547"
     CDS             complement(15079..15378)
                     /locus_tag="ACG81C_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS5064715.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(15480..17126)
                     /gene="mdcA"
                     /locus_tag="ACG81C_001548"
     CDS             complement(15480..17126)
                     /gene="mdcA"
                     /locus_tag="ACG81C_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS5064716.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(17133..18044)
                     /locus_tag="ACG81C_001549"
     CDS             complement(17133..18044)
                     /locus_tag="ACG81C_001549"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5064717.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            18164..19021
                     /locus_tag="ACG81C_001550"
     CDS             18164..19021
                     /locus_tag="ACG81C_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5064718.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         5317 a         4075 c         3309 g         6439 t
ORIGIN      
        1 aacattcact ttggattaca agaatttctt tattgtgtta ataaagtttc ttcattctag
       61 tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt ctttgatgaa
      121 ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat cgaccatttt
      181 tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa gcttctcact
      241 atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat ccactgtttt
      301 tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa ttgccttcag
      361 ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct ggttcttcca
      421 tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc ggtggtccta
      481 atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat tctaattctt
      541 ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt tcagaaattt
      601 ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg acataaagca
      661 tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt ctttcctgtc
      721 tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat actattcaag
      781 aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt acaaaaaaaa
      841 aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat
      901 ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca
      961 acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca
     1021 gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc cagttgagac
     1081 agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat
     1141 cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat
     1201 ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac
     1261 tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt
     1321 tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct
     1381 gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg
     1441 cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt
     1501 ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga
     1561 aattggtatc cactattact tttttcatct gttcacacct cttgattaga ataattgctt
     1621 gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt
     1681 gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag
     1741 ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt
     1801 accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg
     1861 atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc
     1921 ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt
     1981 ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc
     2041 aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt
     2101 catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc
     2161 ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta aaagatattc
     2221 caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt
     2281 ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat
     2341 ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat
     2401 tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat
     2461 tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc
     2521 tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag
     2581 gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac ctctttttgt
     2641 tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat
     2701 attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa
     2761 ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta
     2821 aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc
     2881 gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc
     2941 gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact
     3001 tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac
     3061 cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca
     3121 cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct
     3181 tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca
     3241 aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt
     3301 agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta
     3361 tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc
     3421 tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg
     3481 ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa
     3541 atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac
     3601 aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat
     3661 caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc
     3721 tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt
     3781 gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt
     3841 gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc
     3901 ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat
     3961 cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt
     4021 cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg
     4081 tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac
     4141 taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt
     4201 tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa
     4261 acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg
     4321 gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca
     4381 gaagaatgaa atactttttc atctcattta tttcctaagt taattggact ttccaagttc
     4441 ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca
     4501 ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc
     4561 cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca
     4621 ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac atcaactact
     4681 gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga
     4741 ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg
     4801 tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg
     4861 ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact
     4921 gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa
     4981 gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc
     5041 aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc
     5101 atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct
     5161 tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact
     5221 gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat
     5281 tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat
     5341 ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc
     5401 tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt
     5461 attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa
     5521 tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat
     5581 ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc
     5641 tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa
     5701 catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata
     5761 taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga atcccatcga
     5821 attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc
     5881 gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc
     5941 ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt
     6001 aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg
     6061 gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat
     6121 agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa
     6181 tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg
     6241 atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag
     6301 cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg
     6361 caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac
     6421 accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc
     6481 agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc
     6541 gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac
     6601 tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt
     6661 tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc
     6721 tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat
     6781 cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat
     6841 gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat
     6901 gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt
     6961 gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtttttacc
     7021 ctcttttctt tttgtattta gataactccc ttatcttgca gaactggggt gattttttct
     7081 tcaatttcat tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact
     7141 gcgaactttt cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg
     7201 gaaactgcgg aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa
     7261 acatcctcag cagacatctg tgctgcaaaa ctacttccta aacctgcacc gcatacgaat
     7321 aaaatgttca atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact
     7381 gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc
     7441 tcttcgttgt tgatcaattc aattagttct ttcatgatgt tcagatgcga gtaggagtct
     7501 actgctgcca aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt
     7561 ggttgctcaa tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca
     7621 tgggctagtg ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga
     7681 atcatcgctt cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct
     7741 agttcaatcg attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc
     7801 aaaatatctt ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg
     7861 aataattttt ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg
     7921 acatctatta tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt
     7981 agttcttcat ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc
     8041 ggatcgacaa ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga
     8101 ttgacatcca atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg
     8161 atctgaggaa acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg
     8221 ttacagacaa cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc
     8281 gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact
     8341 tcttcgatcg taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg
     8401 atgttttctt tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca
     8461 gccatgtgct tatagagatt ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt
     8521 gtctcgttct ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg
     8581 attgaattgg agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt
     8641 tctacttgcg ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa
     8701 aagctactgt cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc
     8761 ctgattcgct ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct
     8821 tcttgtttca acaacttatt ggaatagatc tcctgtattt ttttgatttc caccaaagga
     8881 aaataatgga gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag
     8941 tcggtcattc ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct
     9001 gaacctttat aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc
     9061 agtgttgctc gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct
     9121 tcttcttttt tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca
     9181 aaaatacgtt cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat
     9241 cgagcttgct tttccttttc cagcttcaac aaaaaacctt tgttgcgaat tgtacggatc
     9301 tccggaagct ttttactgct caaccagata ttcgcttcaa gaacttcctg acggatcgtc
     9361 cgctgactga cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc
     9421 atgattctgt gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt
     9481 caagtatatg gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa
     9541 gaaagtaagc aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa
     9601 gacatacgcc tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa
     9661 gctgtcctgc atcaagtaat aagaacaact aaactaaaat agtgcttcct atttttcgtt
     9721 tggttgttct tattatcgca gatgcaagct tttaaacacc atttagtgga gaggttgtga
     9781 cagagatgtt cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta
     9841 caaaatcgtg ccttattgcc gataggctat ctctggcaca ccgtttattc agaaaaagga
     9901 gctgcgacaa ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa
     9961 tcggctgaat aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata
    10021 tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat
    10081 cgactgaata atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct
    10141 gtcgataatt tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg
    10201 ctttgttttt ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc
    10261 tggctttttc aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga
    10321 tttccagaaa ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg
    10381 ctttcagcct tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta
    10441 ctgacaaaac agctaatgca agaaatcccg tttgctcata actagaagga cctgtacctg
    10501 gaccataata cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat
    10561 atgaacgacc accaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat
    10621 gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca
    10681 aaatgattgc taaataattg atttttttca ttgtcttcct cgttatcttt tattacaaaa
    10741 aactattttt atgatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt
    10801 tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc
    10861 agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc
    10921 tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg
    10981 taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc
    11041 actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat
    11101 ttgtttctgt acatatgaat cattatctag ctccttagct gcttgaattg caagcctgat
    11161 atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac
    11221 cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt
    11281 gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc
    11341 aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt
    11401 tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa
    11461 ctcctgatca atagcatgat gccatgctat ttgagccatt tcttcaaaaa aaggagttag
    11521 cgtcttagca gacttaagaa ataaagcata gtccatatcg gaatgtgcac ccgcatccag
    11581 tgcatccact aatccaggtt ttggacttaa tgttacttct gcgatcaatg cttcctctac
    11641 caacgcagcg agttgctgag tcactgcttt ttttcgttcg tcatttatca tgacactcac
    11701 tccttcttcc tactattact tgctgaaacc ttttttgata gctacccaaa tgatatgaag
    11761 taaccagctt aaagcaaatg cacaacttgc cgtcattaga taagctacaa gaatcatgac
    11821 tcctgaaggt aactgcagcc aactatcttt aaacaataat aacagtactt ggaaaacaaa
    11881 tacattggct aaatacgtac gataagcata aacagacaac catttgaaca atggcaattt
    11941 tggcgaatcg tttacaatca ttttggaagc gcctatgaag acagcaaaga tgatgactaa
    12001 actgtataaa ctttggatcg tattcaagta aggtgcattc gcaaagctca agtctcctgg
    12061 atgtgcgagc aatgaacgga cagaaaagaa cgcgataact aaggcaacag gtaagaaaag
    12121 ataacgtact ctgtccaaat attgatagac tttttcgtga taaacaaatg cggctacccc
    12181 ataaatcatg aaactagcaa caaaccgatc taataagtac cagctgtcat gataaggccc
    12241 agtcaatact tgtgtttgat agaacagata ccatgcgaca tataaaatcg ttgaaccaat
    12301 caatactggc catacatatt ttttattagc aaatactttg taaccgagcc aaacaaaaaa
    12361 cggcatgaac aattggatct gcagcatcat aacggtatac cataaatgag gcgctccatc
    12421 acctgtgaca tatttcagta aaaagcttgc agtatccgta tacggagttt gttgttggac
    12481 tgctggaaat agccaaagat aagccgtcgt ccatagaaga tacggaacga ctagttcaaa
    12541 aaacttttct ttcaaaaatt cccaataaga caaatgctga cttgttttta ccatattata
    12601 aacaattgca aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag
    12661 aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca aagctcaata tcgtttgcat
    12721 cataacagcc gttacagctg atactttctg gaagtcactt acgttgattt tgattttttt
    12781 gttttcaaac atctctttct ctctcttttt ctttattacc aaagttcttt ggttaattta
    12841 ggtccttcga ctgttttcaa tagaattttt ttctctggct ctctcaaata ctcttttagt
    12901 gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc
    12961 gattgaatta cctgaattgg caattcaatc ggtgaatttg cgtacagcag caaatcaaaa
    13021 tcactggttt cttttactgc cggaatccca gtcgataatt caaatcctaa gctgccgccg
    13081 attccccatt tacatcctga taatatgttt ctagcagcat gaaattgctg ataaacagga
    13141 taatccatga ttttctcttg tctatacgat tcttgttcga tgatgtccca cggatgaatg
    13201 acttgtttga cggcatgttt tgagatttct gttgaccatc tctgattgcg ggcaggtcca
    13261 cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac
    13321 atcttttcag ttagaacgac ccattcaggc aaggtgaacc cttctatttc atgtttatca
    13381 aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat cccattcttc gtccattttg
    13441 gcacggactt tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga
    13501 tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt gattgcttcg
    13561 atgactttgc tgacattttg ttctgaagca gcttggtcgg tgatatcttc taggaaatga
    13621 tagatcgctc ctagttttgt ataattattg atatcgtaag cgatagctgg aactttttca
    13681 gcagctgctt cgatttcagc aaccgaacgt tttgtcacac gtgcagcact ttctttagac
    13741 atcgcttgga cggtaatttc tttattgttt aaagcaatca agcggcttga ttgtaatcca
    13801 tgtgctaaaa atgcacctga aatagcattt cctgcaatca atccaataac tggatgtcca
    13861 gcttgacgga gttttgcata agcagcagca ctattagcca aagccacatg gataccgatc
    13921 aattcttcgt tatagccgta tgcttggctt ggtacatcga tcacaacgat gattggccgt
    13981 ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa
    14041 ccttcaacta aaccaacttc accgtttcgt acacggtaca tcgggttttt aggatcaggt
    14101 acgattgatg tcacgatggc ttcttctatg aatttacttg atttaccata atacacagta
    14161 ctaatagaag aagttggatt tttcatgcca gttagtgccg caaaccaatc gtaaccaaca
    14221 cttgctgcag ctggttcatt tccttctgct acttctggta agtcaattga ttttgcttga
    14281 tggttagcaa ttgcttcatt gtaatcgtca atggccattg gttttgaaag atctaaagaa
    14341 tcaagtaatg ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc
    14401 attgctgcga tgacagcagc tttgattgct tctacactat cagtcactaa ttcatcaatg
    14461 atttttgatt ctacccgttg acgagatcct aatgtattcc aaatcaagtc tttgtcacta
    14521 gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt caaaccgact
    14581 ctcgcttttt ttgttgcgat taaataactc attaatgctg aagtaatcga cataccgcca
    14641 aatgatccta cacgtcctgg aatcaaaccg atgactggta catattgttt tagagcaatg
    14701 attacatttc cgatttctga aatagataac agtccgtagt ttgcttcttg caaacgaaca
    14761 ccacctgtat ctagtacaat gactggaaag atctcatttc cttttttatt ttcttctaac
    14821 acatgttcaa gtgcagcaac gatttttgca ccggaaactt caccgatacc gccaccttgg
    14881 aattttcctt ccatagcgat tactactgct tgtttgccat tgatcgtgcc acggctgaca
    14941 acgatcccgt catcactttc agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt
    15001 tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg
    15061 gcgtttaatt ctacaaaact atttttcatc tgaaagttcc tccattgctt gcgtcaatcg
    15121 taggtacaca actcctggtg tagcaccgaa gtcattgatc acgatatctg ctgaaattgg
    15181 ataacgatca aaaaatctcg ttaatacatt gttccatact tcgccgaagc catcgcttcc
    15241 tgtacaaatt tttacagtag attctgtttt ttctgttgga aacatcaaga tttccaaatc
    15301 tcctgaaccg accactccta catggatttt cttattgatc ggctgtttcg ttggatattt
    15361 ataagctaat tgttccatgg atctctcccc tttttgtaaa acagagcata cacgttctgc
    15421 ttattcgtct ttcttcttcc atctgggtaa atggcgacaa aaggtttttc tcgccaattt
    15481 tatgaccaag ttctgaattt cattggtggt tcatacaggc catctgacca ttcgatcaaa
    15541 tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt cactacgttt gatgcctaga
    15601 tcttctggta atttcacgat gccggctgca cgcaagctat ctagtgattc tttcgtgcta
    15661 gtcattccaa gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg ttcttctttt
    15721 gtttttgctt tgtacaaata agcaattcct tcttctgtca cgatatgtgt agtatcttcg
    15781 ctataaatca tcactggtgc attggctaag cctgcttgtt tttgaacagc tagtgcatct
    15841 aattcttcga caaagactgg ttttttattt gaaccatatg tttcaaccat ttgaacaact
    15901 aatttttggc ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta
    15961 gaatgacgac gaccgccagg attatgtccc atgttaggcg caccgccaaa accagataaa
    16021 cgtccttttg ttacggtaga agagtttcct tcgtaatcta attgcaaggt tgaaccgata
    16081 aacatatcga tggcatattg tcctgcagct tgtgataatg ttcggttact gcgcatcgtg
    16141 ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa tatatttttc cataccaact
    16201 tcgccaccaa aactatggat gctttcaacc caacctgatt cgatggctgg aatcatcgtt
    16261 ggatgtgggt tcaataccca gttcttcgcg attttccctt ttaaccctag tgattcacca
    16321 taagttggca gtaacaattc gatagctgct gtattgaatc caataccgtg attcaaagat
    16381 tgtacgccgt gttctgcgta gattcctttg attgccatca ttgccattag aatttgcaat
    16441 tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta attgataagg tttatctgct
    16501 ttcacgacaa cgtctaccca gtcaccaggg atatctacac gtggtaaaga atcgacaact
    16561 tcgttaactt gaacgatcac gataccatct ttgaaagctg cagcttcgat gattgttggt
    16621 gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt atctgcagct
    16681 actaaaacaa catttggaat caaatcgatg aataagcgtc cataaagttc caaataagta
    16741 tggatatcac ctaatttcat tgtgccgtct tcaatcattt gggcgacacg caagctttgt
    16801 gcaccagcat aagagaaatc gatcttgctt gcgattcctt tttcaaaaat attcaaatgt
    16861 tctggtcgtg agatactaga catgatcaaa tgcagatcat tcactttttc tgcatctact
    16921 ttttcaagac tttctgaaag aaaacttgct tgtttttgat tgtcgccttc taatacaact
    16981 ttgtcgccgg gagcaatcag tttttccatt acttctacga ttttttcagt ttctacatac
    17041 ttgccattga gcaagttgct gattttttcc aatcgctgcg cttttttttc gcgtttagaa
    17101 gcccagttgc ggtttggttt ttccataatt agtcatctcc atttttgata tagaaacgtg
    17161 gcgtcatcaa taccatgttg gtctagattg atcgtacgga tccctttatc tacaccaaat
    17221 tttgtatgaa tcagctttgt caaagtattg ccggggggaa attcaatcgt cagatccatt
    17281 cctaattctt ttgccaagtc catcatttgg ttccactgca cagggaaagc taggttttcc
    17341 aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt attttttata
    17401 tacaaacatc tagcatcaga tagatggaca gactgcatat acggcttgaa tttcttgacc
    17461 gtatcattca tcaagagaca gtgagatggc acagggactt tcagcagctt cgctgtttgc
    17521 gcttggtgcc gtttggcttc tgccaacgta acggataaac cttcgatact cccagataac
    17581 gtatgttgca tcggacaatt ttcatttgac agataaactg gatggtcagg atcaaatgtt
    17641 tttgctacaa tttcctcagc ttcgcttctc gtcacaccta ctactacgcc cattgcataa
    17701 ccgcttggat aagcttcttt cattaagact gcgcggtgat agactagttc cgctgcattc
    17761 tcaaatctca aagatcgaca agcaacagct gcacaaaaag caccaatcga atgtcctgct
    17821 gcaatatcag gctgaatacc gtttttttcc atttcatcta aataaaatac tgcttttgtc
    17881 agtaatgcta gctggataaa aaccgtatct tgatagtttt cttctttatc aactaaccga
    17941 taccccgtca cattttctaa acgttccatg tacatttcag ggatatccgc taacatccct
    18001 aacttctgac tgccttgtcc aggaaataag aaacctgtac tcatgagcct cgcctcctca
    18061 gcacttactt tttccactgc tatcttagtc aatttctgct ataataaaaa atgccaaaaa
    18121 aagaaaaaac cataacaaaa aggtatggat gggatggata attatgaaat tgcttcatct
    18181 gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca aagaattatt
    18241 tatttctcaa cctgccttga gccgtgcgat caaacatttg gaaaacgaat taggtgtccc
    18301 tttgttttac catagcggca gaaacattga gacgaccgtt tatgcagaag aattttatcc
    18361 atatgcagtc aagacattag acacattaga agaaggaatc aaattattgt cttcggtcaa
    18421 tgagaagatc gttacttccg tcatattgta tttagaggta gcctctgttt ctattccgaa
    18481 cttggtccga atttttatgg aaaagcatcc tgatatccaa ttaacgatca tgcaacatca
    18541 tttaccagat gatattaccg atcctgtact gtacatcact tcggagatga agcctggcat
    18601 gaccaatatt cctatcatga aagaacctgt ttatgtcgcc attcctaaga aacatcctct
    18661 tgctaaaaaa gaacatctgc aattagaaga tatccaacgc acccccatct tgatgctgtc
    18721 aaagagcaac gccttccgtc atacactgga tgcagcactt gaagcgaaaa gtatcgaatt
    18781 gacgatcagt tcgacgacag atgatcctgc gacattacgt tctattgtca agcaaggatt
    18841 agctgttagc tttttcccaa aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt
    18901 gcgggaaatc aaagatttgc ccttgacacg aacaatttat ttatcttcta gcttcacaga
    18961 agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt taggaaaata
    19021 aaaaaaaaag agctgggaca aaattcttgt cgcagctctt ttttcgaaca aacggtgttc
    19081 cagaagtaat ttcttagaaa ataagccgaa atttcccaaa aatttgagaa gcaattttcg
//
LOCUS       DAZUMI010000052        18564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000052 DAZUMI010000000
VERSION     DAZUMI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.052"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG81C_001551"
     CDS             6..305
                     /locus_tag="ACG81C_001551"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5064719.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG81C_001552"
     CDS             378..1739
                     /locus_tag="ACG81C_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5064720.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG81C_001553"
     CDS             1761..2021
                     /locus_tag="ACG81C_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064721.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG81C_001554"
     CDS             2035..2787
                     /locus_tag="ACG81C_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5064722.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG81C_001555"
     CDS             complement(2881..3486)
                     /locus_tag="ACG81C_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5064723.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG81C_001556"
     CDS             complement(3539..4513)
                     /locus_tag="ACG81C_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5064724.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG81C_001557"
     CDS             complement(4891..5610)
                     /locus_tag="ACG81C_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5064725.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG81C_001558"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG81C_001558"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5064726.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG81C_001559"
     CDS             7161..8558
                     /locus_tag="ACG81C_001559"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5064727.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG81C_001560"
     CDS             8639..9079
                     /locus_tag="ACG81C_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5064728.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG81C_001561"
     CDS             9227..9553
                     /locus_tag="ACG81C_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5064729.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG81C_001562"
     CDS             9554..10303
                     /locus_tag="ACG81C_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5064730.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG81C_001563"
     CDS             complement(10383..10838)
                     /locus_tag="ACG81C_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5064731.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG81C_001564"
     CDS             complement(11265..11540)
                     /locus_tag="ACG81C_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064732.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG81C_001565"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG81C_001565"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5064733.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG81C_001566"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG81C_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5064734.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG81C_001567"
     CDS             14770..17436
                     /locus_tag="ACG81C_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5064735.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG81C_001568"
     CDS             17800..18552
                     /locus_tag="ACG81C_001568"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5064736.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5964 a         3212 c         3832 g         5556 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct tttt
//
LOCUS       DAZUMI010000053        18285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000053 DAZUMI010000000
VERSION     DAZUMI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.053"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      27..193
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            341..898
                     /locus_tag="ACG81C_001569"
                     /pseudo
     CDS             341..898
                     /locus_tag="ACG81C_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            956..2995
                     /locus_tag="ACG81C_001570"
     CDS             956..2995
                     /locus_tag="ACG81C_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5064737.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3104..4255)
                     /locus_tag="ACG81C_001571"
     CDS             complement(3104..4255)
                     /locus_tag="ACG81C_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5064738.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4409..4939)
                     /locus_tag="ACG81C_001572"
     CDS             complement(4409..4939)
                     /locus_tag="ACG81C_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5064739.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5038..5388)
                     /locus_tag="ACG81C_001573"
     CDS             complement(5038..5388)
                     /locus_tag="ACG81C_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5064740.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5545..7074)
                     /gene="ahpF"
                     /locus_tag="ACG81C_001574"
     CDS             complement(5545..7074)
                     /gene="ahpF"
                     /locus_tag="ACG81C_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5064741.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7084..7647)
                     /gene="ahpC"
                     /locus_tag="ACG81C_001575"
     CDS             complement(7084..7647)
                     /gene="ahpC"
                     /locus_tag="ACG81C_001575"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5064742.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7791..8453)
                     /locus_tag="ACG81C_001576"
     CDS             complement(7791..8453)
                     /locus_tag="ACG81C_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5064743.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8637..10283
                     /locus_tag="ACG81C_001577"
     CDS             8637..10283
                     /locus_tag="ACG81C_001577"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5064744.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10274..11356
                     /gene="uxuA"
                     /locus_tag="ACG81C_001578"
     CDS             10274..11356
                     /gene="uxuA"
                     /locus_tag="ACG81C_001578"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5064745.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11464..13017)
                     /locus_tag="ACG81C_001579"
     CDS             complement(11464..13017)
                     /locus_tag="ACG81C_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5064746.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13165..14400
                     /locus_tag="ACG81C_001580"
     CDS             13165..14400
                     /locus_tag="ACG81C_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5064747.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            14373..14453
                     /locus_tag="ACG81C_001581"
     CDS             14373..14453
                     /locus_tag="ACG81C_001581"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064748.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14579..15658)
                     /locus_tag="ACG81C_001582"
     CDS             complement(14579..15658)
                     /locus_tag="ACG81C_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5064749.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15761..16201)
                     /locus_tag="ACG81C_001583"
     CDS             complement(15761..16201)
                     /locus_tag="ACG81C_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5064750.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16258..16668)
                     /locus_tag="ACG81C_001584"
     CDS             complement(16258..16668)
                     /locus_tag="ACG81C_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5064751.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16848..17282)
                     /locus_tag="ACG81C_001585"
     CDS             complement(16848..17282)
                     /locus_tag="ACG81C_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5064752.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17382..18230)
                     /locus_tag="ACG81C_001586"
     CDS             complement(17382..18230)
                     /locus_tag="ACG81C_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5064753.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6285 a         3395 c         3308 g         5297 t
ORIGIN      
        1 atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc ctatacaaac
       61 ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt
      121 aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta aaactcggac
      181 gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt ttctttatat
      241 ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag tggattgctg
      301 tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ggtcgctcgg attcacttct
      361 ggttgcacaa tacaaccaga aaacacaaca agcaaaactg atctctatca tgagagattc
      421 atatgtcgat attccaggtt acggaatgaa taagataaat gcagcttact cttatggggg
      481 agttgattta ttaaatcaaa cattaaagga aaatttcaaa tttgaagctc cctattatgc
      541 aagtatcaca tttcaagatt ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa
      601 gatcgatgca gaaaaatctc tagatttaga tggcgtatat ataaacaaag gagagcaagt
      661 gatggatggt aatactttac tccagtatgc tcgatttcgg gaagacaaag aaggagactt
      721 cgggaggatc agaaggcagc agcaagtgat caaagctatc tctcagcagc tgaaagacgt
      781 aacttcaata ttcaagctac ccaaagcggt tgggaaatta cttggtagta tccaaacgaa
      841 ccttccagaa agtgtgcttc tggattgtgg tatggatttc ctcaaagaca tatgttagaa
      901 taaacaggta taaatagtga aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa
      961 aagaagtgac aagcacggca aaaatcgaac aggcgcttat attgccggcg cagtgatctt
     1021 aatagcagct gcgggtggcg gttattttta ctaccagcac tatcaagaaa cccaagcagt
     1081 agaagctgga gaaaagacag ttgagcaatt tgtccaagct ttgaacaagg gagattataa
     1141 caaagctgca gaaatgacat caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga
     1201 gatcttagat aaataccaga atatatacgg tgctgctgat gtcaaaggac ttcagatatc
     1261 gaatctaaaa gtagataaaa aagacgattc tacttacagt ttttcatata aagcaaagat
     1321 gaatacctca ttaggtgaat tgaaagatct ttcttataaa ggaacattag acagaaatga
     1381 tggtcaaacc acgatcaact ggcaacccaa cttggttttt ccagaaatgg aaggaaatga
     1441 caaagtaagc ctgaccacac aagaagcagc aagagggaat attatagatc gaaatggtga
     1501 accattagca acaacaggca aactaaaaca attaggagtc gttccaagta aacttggaga
     1561 tggaggcgaa aaaacagcca atatcaaagc gattgcttcc tccttcgact taacagaaga
     1621 tgctatcaat caggcgattt cacaaagctg ggtacaaccc gattactttg tcccattgaa
     1681 aatcattgat ggagcaacgc cagaacttcc agctggagct accattcaag aagtagacgg
     1741 cagatattat cctttgggtg aagcagctgc tcagctgatt ggttacgtgg gagatatcac
     1801 agcagaagat attgataaga atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg
     1861 tttggagatg gcttttgata aggatcttcg tgggacaaca ggtgggaaat taagcattac
     1921 agatgcagac ggtgtagaga aaaaagttct gatcgaacat gaagttcaaa atggcaaaga
     1981 tatcaaattg acaatcgatg cgaaggcaca aaaaatagct tttgacagtc taggaggaaa
     2041 agctgcatca actgttgcga caacgccaaa aaccggtgat cttcttgcgc ttgctagctc
     2101 tccaagctat gatccaaaca aaatgacaaa cgggatctca caagaagatt acaaagctta
     2161 tgaagaaaat cctgaacatc cattcatcag ccgatttgcg acaggttatg ctcctggatc
     2221 tacgtttaaa atgattacag cagcaatcgg tctcgacaac ggcactatcg atccaaatga
     2281 agtgttgacg atcaacgggc ttaaatggca aaaagacagt tcttggggat cttttaaaac
     2341 aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa actgctttga tctattccga
     2401 taatatatat gcggcacaag aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt
     2461 agataaattc atttttggtg aagaccttga tttgccaatt agtatgaatc cagcacaaat
     2521 ttctaatgaa gatagcttta actcagatat cttgctagct gatactggat atggacaggg
     2581 cgaacttcta attactccta tccagcaagc agcaatgtat tctgttttta ccaacaatgg
     2641 cacacttgtc tatcctaaat tgattgcaga taaagagaca aaagataaga agaatgtcat
     2701 cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa
     2761 tggtacagca cattctcttt ctgctttagg gattccgttg gcagcgaaaa ctggtacagc
     2821 ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac agtttcttgt ttgctttcaa
     2881 tcttgataac caaggttata tgatggtcag catgttggaa aataaagaag atgatgattc
     2941 agcaaccaaa cgagcatcgg aactattgca atacctcaac caaaattatc aataaaaaat
     3001 tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc acagtccttt tttcaaataa
     3061 acggttttca cgatgtctct ttcacaactt ctttttgatt tactcacact tcataaactt
     3121 tttttgtaaa atatttgaga acaagtggag aaataagtgt acttaataaa atcacgataa
     3181 ctaaaggtga atagtactgt ggttcgatca attgactttg ctgtccaatt tgtaaaatga
     3241 tcaaagccat ttctccacgc gaaatcatac cagcaccgac catcaatgaa ctatgttgag
     3301 aaaaaccggc aatctgagca ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa
     3361 gcgtcaatat agaaataaag agtagttgtt cactaaagtc agaaaaatca acttctaatc
     3421 ctacactgac aaaaaatact ggtataaata ctgcatatcc taaagcctct acattgtggt
     3481 atacttcttc tttaaccttt gtctgactga cagctatccc agcaaaaaaa gcaccaatga
     3541 cagagctaag cccaacaaga tccgctacgt aagacattcc taaacaaata actagcgata
     3601 cgatgataat agaagaattt gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa
     3661 tccatttgac taataagaaa ataaaaacaa aataaattag ctgttcaatc aaaacaagcg
     3721 gtaaagaagc attgctagaa gcttcccccg ttagaaaaga taggctgaaa cttagaacta
     3781 aaacgactaa aatgtcatct accacagaag cacccaaaat tgttgatcct tcttttgtat
     3841 tcactacgtt tagttctttt aatacttcta ctgaaatact gacagaagtt gccgcaagaa
     3901 taatcccaaa gaaaatcgct tcatttcccg aaacttggaa tgctttgcct gtcaaccagc
     3961 caaaaacaac cgggaagatg atcccaagca aagcaacgaa cataccaggt cggaaatatt
     4021 ttttcaaaag agataaatca ctttccagac cagctaaaaa cattaataag atcacaccaa
     4081 tctccgaaaa gtcatggact agaatatcag gtttgaccca attcaatcca gcttggccta
     4141 gcaaaacacc aacaagtaat tgtccaatca ctgctgggat acctaatctc cgcgagatat
     4201 gtgagccaat cgttgttgaa atcaatatca ggcataaaat accaataaat tccattctca
     4261 tcattccttt gtataaaata tagcccgaac ttagcataac atacattatt gaaaaaatag
     4321 gaaaatatac tcacaaaaaa accaacaatc caaaagattt ttttggattg ttggttttta
     4381 tacggtctat tttaataact aaaaatgatt aacgtatagt catttttctt attctgatga
     4441 aattgataat ctcctggatg cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa
     4501 gcatcaaact tttgaatgga ttgaacaata agacgaatcc gccaatcaac aacagaatac
     4561 tactataaac actccaactt ttagcttttg tattcatgct gaagaacaac tggaacaagc
     4621 tgttgatgat aacagctaat ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa
     4681 aagcatagac gatcagagct aagatcagga aaaaaagacc actgaacaga ccgatactcc
     4741 aagaacctgt tttcctttta aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa
     4801 caagataggc ggtgatcaga taaccgataa aatgaaaaac agcagaaggg tttagcacaa
     4861 tcgcaatccc agaaataata taaacagcag ctcttaataa ggcgtatttt tgaatatttt
     4921 gtttaatcgt ctcaaacata taagaaaacc tcctttgact tattttaacg tgaactagtc
     4981 attttaccaa attaaatgtt acaaaatgag cgaaggagaa gaatatctct ggaaaaatca
     5041 acttctgagg atcttttcca tttttttgcc ttttgctagc tcatcgatca ttttatctaa
     5101 ataacgaatt tctttcatca agttatcctc gatgtcttct actcgtacac cacaaacaac
     5161 acctgtaatc aaggaacgtg aaggattggg gacaggggct tccgcaaaaa attgttcgaa
     5221 agtcacttgt ttttgtagtt gatattctag ttctgtttgg gaataacccg tcagccaaca
     5281 aatgatttca tctacttcct cttttatacg atcttttctc tccgcttttg ctacatacat
     5341 aggataaacg cttgcaaaac ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct
     5401 ttcttttttt caaattattt actacttata gtatataaga aaaagagaag aaaaagcgat
     5461 tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc accatccttt tttccgctct
     5521 ctgacttttt acaatcttca tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc
     5581 tgcagtagct cctgaaccca ttgaaacaat gatttgtttg tatggactat ctgtacaatc
     5641 accagctgcg aaaacgccgg ggatattcgt tgcgccattt ttctcggtca ctatttcatt
     5701 tcttggattc atttcgatcg ttccatctag ccaatccgta ttagggagta agccgactag
     5761 aatgaatact cctgaaacag ctagtgtatg catctccatc gtacggcgat cgatatagga
     5821 gagagattct acctgattcg taccgctgat ttctttcgtc gctgcatgtt tgataacagt
     5881 gacattcgca agagagacta gtttatcctg taagatttga tctgctttta attctggtaa
     5941 gaattctaga acatatacat gttttgctaa cccagccaaa tcgattgctg cttcaacacc
     6001 agaattacca ccaccgataa cagcaatctc ttttccttta aataaagggc cgtcacagtg
     6061 tgggcaataa gcaattcctt tatttttaaa ctctttttcg cctggtacat tgatagaacg
     6121 ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta agtgatatac cgttagctaa
     6181 gccaatatta ataaaatgtt gctctttatt taaggaaacg gcctgttgat tcatcataat
     6241 gtcgataggg taactacgca catgactttc tacttgctgc atcaattttg gaccttctgt
     6301 gtaagaggta ccaatcatat tttcaatccc tagtgtttca acgacttgtc cgccgaaaga
     6361 atcagcgaca agtcctgtac ggattccttt acgtgctgca tagatagctg cactactagc
     6421 tgctggtcca ccgccaatta ctaatacatc ataaggttct ttttcaaaca actcttcttc
     6481 aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa
     6541 ttcttctcca tttaaaaaca cagttggcac tgccatgatt tttttatcat cgatttcttt
     6601 ttggaacatc ccaccttcaa tcatcgtatg agtaatattt ggatttaata cagctaatat
     6661 attcaatgcc tgtacaacgt cggggcaatt atgacaagtc aagctaacat aactgtcaaa
     6721 atgcaatgtt tgtgttattt ttttgatttt aagactcaaa gattcactga ttttcggtgc
     6781 ccgtccacta atttgtaaca tagccagtaa aaaagaggaa aattcatgac cgagggggat
     6841 accagcaaaa acgattccac tttctttgcc aactggattg atagaaaaac taggtgtgcg
     6901 catcgaatct cttttttccc aagagatttt aggagacatt tctgctattt ccttcgtaaa
     6961 ttcagctagt ttttgactat tttcactatt atccaaacta acagaaaaaa caataggaga
     7021 ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt tctttgtcta acatatccac
     7081 cacctaaatc tttccaacaa gatcaaaact tggtttcaat gtttcgccac tttctttcca
     7141 ttttgccgga caaacttcac ctgggtgggt acgtacatat tgagcagcgc gaatcttatc
     7201 aatcagtgca ctagcgtcac gaccgattcc atctgcattg atttccatag cttgaacgat
     7261 tccgtctgga tcaataataa atgttcctcg ctgagctaat ccttgctctt catccaaaac
     7321 atcaaaaata cgagaaatct gatgagacgg atcaccgatc atcacgtatt cgattttacc
     7381 gatagcatct gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga
     7441 aaagacttca acacctaaag atttcaatgt actgtattgt tcttgtaagt cttctaattc
     7501 agtaggacaa acaaaagtaa aatcagctgg atagaagcaa agaatactcc agttccccat
     7561 aagatctttt tcactgactt ctaaaaattc tccttgatga taagcatgtg cattaaattc
     7621 ttcgattttt ttcccaatca atgacatagt aaaacctcca ataataatta tttatttata
     7681 attattgtaa tttagttgga gttgcaaagt caatggatat tttgttattt aataaagatt
     7741 actcaaaaac gaaggttaat tataaaaata gaagaggtcg caagaaaatg ctactgctgg
     7801 aaatactcgg gataacgttg agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc
     7861 aatcccgttt gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt gagtaaatca
     7921 taatgttgtt ccatgatcgt ttcccatgga agcccctgaa tggtcactcg taaccagcgg
     7981 aaacgttcta aatgggtatt atggtcatct aaccagttcc aaacttcttt tcttcctgca
     8041 atttcgaaac aagcttcatg aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt
     8101 attttgactg ctttttcttg ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg
     8161 tccaatttgg aacaacattc aatcatgatt tgctcttcca actgttctcg gacaaagcga
     8221 gcattttctg cagaaacaag attaattttg gaaacatacg taccactttg aggtatcgta
     8281 tacacgagtt cttgctgacg tagttgaatc aatgcttcgc gaatgggagt ccggccgata
     8341 gcaagatgtt cctccagtgc cttttcagat attttccttc caggttctaa gtcagcatag
     8401 ataatttgtt tacgaatcgc ttgataagct tgtgattgca agctgctttt catctttatt
     8461 cctccaaaat catttctatg aacgtagtat aacagaagag gtctgaaaaa caatctggta
     8521 tgtcacatta actagtaaat ttgtataaat agtaaaattt tggttgacat agcgattttt
     8581 attgtataaa attcacttga atacaaaaac taatatgtta gtttaggagg ggaaaaatga
     8641 ttgatttaaa caactggagg aactttatcc ctgaattatc tcaaaaagaa gtagaaatac
     8701 ctcattttga ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc tggatccatt
     8761 tcggtggcgg aaatctttat cgaggatttc atgcagaaat cgctcaacgt ttagcggatc
     8821 taggcgaatt gtccactggt gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa
     8881 gggcttacca agattttgat gatttgattc ttgaagtggt catgccaagc agtggagagt
     8941 tcgtcaaacg tatattagct gcgacagctg acagtctgta ttgtcatcca gattgttctg
     9001 aacactttga taagatgatt cgacactttg aaaatcctag tttacaatta gttacttttg
     9061 ctattactga aaaaggttat cgattgaaag atacaaataa taaatatata ggaccgattg
     9121 cagtcgatat aaaaaatggt ccaaagaagc catcacatac aatgagtatt cttgctgcgc
     9181 tactttataa acgtttttta tctggagctt ggccgattgc acttgtctct acagataatt
     9241 tttcacaaaa cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa
     9301 aggaaggtta tgtacaaaat acatttatcc aatacttatc gaatgaagaa attgttgcgt
     9361 ttccatggac gatgattgat cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt
     9421 tggcaaaaga gggcttttca aatatgtcga ttttccaaac cgcaaaagga aataatattg
     9481 ctccatttgt caatacagag gacatccatt atttaatcat ggaagatcat tttcccaatg
     9541 gacgtccgaa attcgaaaaa gctggggtat tcttgactga tcgtgcaacg gtagataaag
     9601 cagatacgat gaaggtgacg acttgcctta atcctttgca tacgtcttta gctattatgg
     9661 gttgtttact ggggtatagg agcatagctg aggagatgaa agatccagat ttatcgctgc
     9721 tagtttctga aatcgggtac aatgaaggcc tcccagttgt ggagtctcca ggtatcattg
     9781 atccaaagat ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca aatattcctg
     9841 atatgcctca gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt tatggtgaga
     9901 cgattaaaaa atatatggaa atagaagtta aagacccgca gacacttgta ttcataccat
     9961 tgactctcgc tgcttggctt cgatacctta ttggcattga tgatcaagga aaaccattca
    10021 tcccgagtcc agacccacta ttgacagact ttcagcataa gctttccgat attcaactgg
    10081 gtacgcaaga tacatcacat attcatgaga aaatggctcc tattttgtca aataaagaaa
    10141 tttttggtgt agatctttac caagcagagc ttggcagaaa aatagaagaa atgttcatca
    10201 agatggtagc aggaccgaat gccgtacgaa aaacattaca ggattactta caagaacatg
    10261 gaggaaattt atcatggaaa tgacttttag atggtacgga tcacaggaag aaaagattac
    10321 cttagatgaa atcagacaga ttcctgcggt caaagggatt gtaggcacat tgatggatat
    10381 accagtagga gaggtatggc ccagggagag gatcaaagaa ctaaaagaag aaattgagtc
    10441 tcgtgggtta acactaaaag taattgaaag cgtcaatgtg catgatgcca ttaaaattgg
    10501 attaccttcc cgtgagcagt atatcgaaaa ctataagcag acaatccgaa acttggcaga
    10561 atatggaata gaagtcatct gctataattt tatgcctgta tttgactggg tgaaatctga
    10621 ccttgactac cgcttagaag acggctcatc cacgttagct tttatatctg atgatattcc
    10681 agatgatcca agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat ttgaattgcc
    10741 gggatgggaa cctgagcgct tagctcatat caaatccttg cttgaagcct atgcatcagt
    10801 agacgaagaa aaacttcgag aaaattttgc ctattttctc caatcaatca tcccaacatg
    10861 tgaagaggta ggggtcaaaa tggctgtaca tccagatgat ccgccatact cgatttttgg
    10921 attacctcga atcgtaaaga atcgagaaga tttagattgg ttgtgccgca tagtagacag
    10981 cccttataac ggaatcactc tttgtacagg gagtattgca gaagatccag aaaatgatgt
    11041 atatgggatt ttagcagaat tttgtcaaag agatcgaatc ccatttgcac atgttcgaaa
    11101 tattaaattt ttagctaatc gagatttcta tgaggcaccg cataaatccg aatacggttc
    11161 attagacatg taccgcatac taaaaacaat gtatgacaac ggatttaacg aatacatccg
    11221 tcctgatcat ggaagaatga tctggaatga gacaggaaga ccaggatacg gcttatatga
    11281 tcgagcatta ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa
    11341 aagtaaagat aattagtgaa aaaaactgta attttattat gtaaataaga aggatgttca
    11401 aattcttgat acataaatcc ggaggttgtt aataagcatt cggatttatg tatcttattt
    11461 ggtctaggaa gtaacttgat tttctttttt cgattgatcc atgagtgctt cttcttttaa
    11521 ttcaaagata tattctgaga tcctcataat cacttttttc cagtcttctg gtgtgatgat
    11581 tccttttaaa atatagattt ttttgatatg agaaggcaat tgtttcaaag ggcagtcgct
    11641 gataacgatg ttgcagttct ccataaaatc attttgtatt ttgatcgatt catttgaaaa
    11701 aatttgttct atcttaacga tcagatcttt actgatataa aaatcacgcg tatagttcac
    11761 accaatcaca attcgcggct gttttttgta gcgatcatac aaatgtacta ggtcttcaac
    11821 catccattgc acattttcct tttctaaaat aactggtaac aagtgttgat tttgttctct
    11881 gaaatccata taaaattgtt caatggcggt ttggacttct ttcaacacgc tatgttcttg
    11941 ataaagcatg aaagtaggaa ggggcgtatt tggttgattg aagttaagaa atccattata
    12001 aagtttattt cgtgcgaatg ccaatgagta aagaatcttt tctttttcag gtataaacaa
    12061 atcaaataca gtacaaaagg actccaccat ttctcttgaa taaagaacat gaggaacgtt
    12121 tgcttttttt aaaagtttgt attgctccaa gcttgtttct tccgattcga ttttcgtgat
    12181 agaaagtcga gctaaaatat tcactaagat gcgttgttcg cgacaaattg gcagattagg
    12241 tcgcaaaata tctacagagg gatcaataaa aaagtcaata tattcatact ctgtgaattc
    12301 tttttgtacg tctttcacag aagttgcagg aaaattctct ttacatattt ttaatagaaa
    12361 atataattta tctatcatac cgctatttag tcggtcaagt acttcttttt taaaaaaagt
    12421 attcatcata aaagccacta cttgcggatc ttcatcataa aattctgtag cagtacttga
    12481 aaagatgttc caataaatat ctaatgctgc atattgaatt tgtacctctt ttccagaaaa
    12541 agactttctg ccattattag aaaaggagat agacaaaccg tacaatgcta aaaatgcatt
    12601 aatctttttc attttcgtat acaaataaga ggcactatag tttgtttgct gacttaaact
    12661 gataatcgaa tttgtctgac agctgaacaa agaagtcaag atctgataac tgactgattc
    12721 gttgcagtat cttccaaata gacgataata gcagtaagcc ggatcaattc cttcaaaatt
    12781 caactttaaa aaaggttttt gcttatcaat atgcagttct gttagattga gacgctcaaa
    12841 gtcttgttct aattctttga tgtatagtaa aaaatttgat tcagacatat ggaaatgatc
    12901 tgctaaatat tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa aaatatctaa
    12961 ttttgtattg ctttttttct tcaagagaaa cgttttaatc gcttcttgag tttgcatgaa
    13021 agaggctcct ttttacttaa attgttaaaa cttgaaaata ttactgtttt tttcataaat
    13081 ttagacaaaa aaacagtaaa catataggta gaatctctac tatcttattt cttgcataca
    13141 aaaaaatcaa ggagtgagag agttatgaga tattcaacca tcttaatatc aacatttgca
    13201 gccttaagct tatcgcttac tataaatcag acagtccaca cagaggagat agaaacagcc
    13261 aaatggacag aaaccaagtg ggccaacgtc aatcgaatta cctttcagga tgaattttca
    13321 tccttcaatt caggagtcgc aaaattaaga aaaggacaaa cagcgaccat caaagcaaaa
    13381 attgactatt caggagactt agcacctata ctacaatcag ctgttacatt tgagaatgtt
    13441 gatcccgctt tgtcgattgg ctatgacgag aacactttga ctatccaaca caagcaagcg
    13501 atatttacct tcaccgttac attaaatcag gacttgttaa aaccagcgct ttttactgtc
    13561 aaggtatcag atggactacc gaacgatcat catcatttaa cgccgtatag tcagcggcag
    13621 acaattgagc aagattcagc tatcgactca ggaggagatc ttgttgaaga accaacagat
    13681 aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga cggagaacag
    13741 aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga gccagacaat
    13801 gaatcaaaac ctgaaataac ccctggagaa aaaccagatg tagacccaga agaaaaacca
    13861 gatgtgactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
    13921 gacacagata atgaaactga aaatccagaa aaaccagagg tagccccaga agaaaaacca
    13981 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
    14041 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
    14101 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
    14161 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
    14221 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
    14281 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
    14341 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
    14401 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
    14461 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
    14521 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
    14581 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
    14641 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
    14701 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
    14761 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
    14821 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
    14881 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
    14941 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
    15001 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
    15061 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
    15121 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
    15181 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
    15241 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
    15301 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
    15361 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
    15421 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
    15481 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
    15541 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
    15601 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    15661 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    15721 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    15781 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    15841 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    15901 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    15961 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    16021 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    16081 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    16141 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    16201 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    16261 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    16321 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    16381 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    16441 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    16501 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    16561 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    16621 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa aattaccacc
    16681 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    16741 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    16801 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    16861 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    16921 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    16981 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    17041 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    17101 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    17161 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    17221 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    17281 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    17341 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    17401 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    17461 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    17521 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    17581 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    17641 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    17701 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    17761 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    17821 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    17881 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    17941 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    18001 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    18061 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    18121 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    18181 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    18241 ccttaattca ttaataatac tcttatctta ttataactta ctttt
//
LOCUS       DAZUMI010000054        18149 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000054 DAZUMI010000000
VERSION     DAZUMI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.054"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..199)
                     /locus_tag="ACG81C_001587"
     tRNA            complement(116..199)
                     /locus_tag="ACG81C_001587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Leu,seq:cag)
     gene            727..1227
                     /locus_tag="ACG81C_001588"
     CDS             727..1227
                     /locus_tag="ACG81C_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064754.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1417..1761)
                     /locus_tag="ACG81C_001589"
     CDS             complement(1417..1761)
                     /locus_tag="ACG81C_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS5064755.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1915..2835)
                     /locus_tag="ACG81C_001590"
     CDS             complement(1915..2835)
                     /locus_tag="ACG81C_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5064756.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3022..3513
                     /locus_tag="ACG81C_001591"
     CDS             3022..3513
                     /locus_tag="ACG81C_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS5064757.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3537..5912)
                     /locus_tag="ACG81C_001592"
     CDS             complement(3537..5912)
                     /locus_tag="ACG81C_001592"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS5064758.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6238..6864
                     /locus_tag="ACG81C_001593"
     CDS             6238..6864
                     /locus_tag="ACG81C_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5064759.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6887..7591
                     /locus_tag="ACG81C_001594"
     CDS             6887..7591
                     /locus_tag="ACG81C_001594"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5064760.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7783..8616
                     /locus_tag="ACG81C_001595"
     CDS             7783..8616
                     /locus_tag="ACG81C_001595"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS5064761.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            8813..10741
                     /locus_tag="ACG81C_001596"
     CDS             8813..10741
                     /locus_tag="ACG81C_001596"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5064762.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            10879..11334
                     /locus_tag="ACG81C_001597"
     CDS             10879..11334
                     /locus_tag="ACG81C_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5064763.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            11414..11542
                     /locus_tag="ACG81C_001598"
     CDS             11414..11542
                     /locus_tag="ACG81C_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064764.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            11804..12073
                     /locus_tag="ACG81C_001599"
     CDS             11804..12073
                     /locus_tag="ACG81C_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064765.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12473..12727
                     /gene="rpsT"
                     /locus_tag="ACG81C_001600"
     CDS             12473..12727
                     /gene="rpsT"
                     /locus_tag="ACG81C_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5064766.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(12816..13862)
                     /gene="holA"
                     /locus_tag="ACG81C_001601"
     CDS             complement(12816..13862)
                     /gene="holA"
                     /locus_tag="ACG81C_001601"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5064767.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(13900..16173)
                     /locus_tag="ACG81C_001602"
     CDS             complement(13900..16173)
                     /locus_tag="ACG81C_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5064768.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16166..16705)
                     /locus_tag="ACG81C_001603"
     CDS             complement(16166..16705)
                     /locus_tag="ACG81C_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5064769.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(16841..17059)
                     /locus_tag="ACG81C_001604"
     CDS             complement(16841..17059)
                     /locus_tag="ACG81C_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5064770.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17056..17301)
                     /locus_tag="ACG81C_001605"
     CDS             complement(17056..17301)
                     /locus_tag="ACG81C_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5064771.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(17417..18145)
                     /locus_tag="ACG81C_001606"
     CDS             complement(17417..18145)
                     /locus_tag="ACG81C_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5064772.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5567 a         3496 c         3189 g         5897 t
ORIGIN      
        1 gatttatttg aatctggttc cgttgtttaa aatttttcct atattctttt agataatatg
       61 ttattttctt tctcatcgct ccaggtgtac cttctcttta taaaaaaaca aattatgcgg
      121 ctaagaagac tcgaacttcc acgaggtcac cctcactagc gcctgaagct agcgcgtctg
      181 ccaattccgc catagccgca tcacatcttt tagtttagga ttacatgtgt tttttgtcaa
      241 gaacatattt ttgttctttt tcatttctgt acgccctccc ttatgccatc tacacaaata
      301 attcatccga gcaactccat catctattga gggttggtga aaaaatctgt tttatccata
      361 tgatcgattt caatgttaaa cgaacgactc tgggattagt aactatttta tttttttctt
      421 catttctttt ttcctctttt tctcgattca gctatcttct ttcatttgga tattgattat
      481 ttcttctgtt gattactatc cttctggaat tctagacgct gaagtaattc acgttttctt
      541 tcttctagag atcataagta acgaatgaac gaacttcaaa taattttttt attgtaattc
      601 atatttgtat tgttcgatga ttcttaatac gatatgacac aataaataaa aataggcatg
      661 gctatttaca acaatagtgc tatagtagaa aacaaatgaa taaggttact taaagaggtg
      721 aatacgatga aactgatcaa actatgggaa gcagatttaa agaaagctta tcagctacaa
      781 actagctttg gaccaaatga gagtggattt atcaacaatg cttatggcta tacatttgct
      841 gaatttttag attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag
      901 agttacgttc ccgcaaccgt ctttatttta gaaagtgatg aacatgagta tgtaggaatt
      961 tttaatttgc gccataaact gaacgaaaca ttaagaaacg gcgctggcca tattggttac
     1021 ggcatctccc ctaattatcg taaaaaagga tatgccacac aaggattagc attagtacta
     1081 caagaggcaa aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa aaaaaatctt
     1141 ggcagtctta aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga tgagaaagaa
     1201 tactatacga aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa gggatttgaa
     1261 tgtcctttat caatagaaca catttattta attaacttat aatatagcag acaaaatttt
     1321 ataaacaaag aaacctttta tctcttttct tctagaaagg ataaaaggct tttttgtttt
     1381 tcctatttgc ttctcatagc tagtcagcca agatttctat cgtatcccca acgcaatccg
     1441 tgcataacgg ctcatcttat ctgtcgtcca tgctggatac cagactagtt tgacttctgc
     1501 tttcgttact tctggcactt cttttaatgc gtcatggata gattctgtta aaatatctgc
     1561 taatggacag cccatagttg ttagagtcat cttaatgact gtttctccat tttcaggatt
     1621 gaattctata tcataaatca agcctaaatt gacgatgtcg attcctaatt caggatcaat
     1681 caccatttcc aaggcagcaa gaatacgttc tttgattagt tctatttctt gtccagaccg
     1741 ttcttggttt gcttcgctca tcgtacgaca ctccttaaca cgatttttat ttatcatact
     1801 ctttttttag ctgcttgtaa atgagaattt agaagtaaga ttctcgttta aatttataaa
     1861 aatgttctta ttagatgtaa tagattaaat ttattctaaa ataaacctga ttcctcattt
     1921 tttttgtgaa cgatttttta tttctggcaa tacgccagtt gttcgataca ttttttgttc
     1981 gttttcattt gcaaaccggc caaatactga atgagttggt aaaatattga taaatgcatc
     2041 gtcgtcgatt tcatgaacga tctgatcaag atcatacatt tcgtagcggg tgatgaccat
     2101 catgatcatg ttcccttgtt tttttgaata gccaccgaca gacggcagta atgtcattcc
     2161 acgtaccatc cgttttgcta gttcttctgt tacctcttct ggcttgacgg taacgatcat
     2221 tgctgttact ttttgatgac tcgtgtggat ggcatcaatt acttgagtca ttgcataaat
     2281 ggaaataatc gtatacaagg cactttccca gttaaagaag aagcctgcga tgactacaat
     2341 gataccattt agaatcagca tgtagtttcc gactgttttt ccagttgttt gtgaaagtac
     2401 aagagagatg atatccatcc caccagttgt aaagcccatt tttaaagata atcctacgcc
     2461 aagacctaat aaaacaccgc ctactagggc attcatcaaa atattatcag tgatctgtcc
     2521 gactggaagc agcatcgtca tcaccgatac acatacaaca ttcacaaaac taaggatcgt
     2581 tgctcgtttt cctactttga gaaaccctaa tacaaacaca gggatattca aaagaaaaat
     2641 aaatgttcct gtgtctatct tgatatgaaa ccattcatat aaaagcgagg aaatgatttg
     2701 agcgatccca ttcatccctg cagaaaagac attggctgga atcaaaaaat aatttaaagc
     2761 gaccgcagcg gtcaatccgg ttatcaaaat aaccactatt ttctttaatc tttcattttt
     2821 atgatacgca gtcataattt tactcacaag cacctctccc ctactttggt attccaaatg
     2881 taagaataac agtttatcta ttaaaataaa atacttcgaa ctcaaatgag aagaatttta
     2941 gcaaacaggt aagaatctgc cctaatccta tgataagata gatgtatggt aaatatttat
     3001 atgtatatca ggagggttcc tatggcaaaa gatgctagct tcgacattgt atcagaagtc
     3061 aatacagaag aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc caatcgcttt
     3121 gacttcaagg attctacagt agaaatcaaa ttagagaaca gcaatctagt tgtattgagc
     3181 gatgatgatt tcaaaatcga acagatcaaa gatgtattat ttggtaagct gatcaaaaga
     3241 aatgtgccaa tcaaaaatat ccatttttcc gaatcagaac acgctctagg tggcaaagct
     3301 cgacaaactg ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa aatcactaca
     3361 gctatcaaaa atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca gattagagtg
     3421 acaggtaaga accgtgatga tctacaagct gtgattgctt tattacgtaa acttgactta
     3481 ccaatcgagt tgcaattcac taactatcgt taatcaaaaa acgaggctga cgtctgtcat
     3541 tcctcgtttt tttgatttac tgcttctttt gataaccaaa aacgctgtat tccttcttcc
     3601 aattccccaa cacttcgtac ttctcttgct gtttgccaat gttgtgggaa taaacgtcga
     3661 taagaaaaac tagaaaatcg ttgatctgcc aatacaacga ttccttgatc cgccatgcca
     3721 cgaatcactc gaccaccagc ttgcaagact ttgttcattc caggaagctg ataagcgtaa
     3781 gcaaatccaa ggtgttcctt ctcatcataa tatgatttga tcagttcctg ctcatgattc
     3841 atctgcggca aacctacacc tacgatcata ctgccgatca atcggtcccc tctcaaatcg
     3901 attccttctg aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc agcgggattt
     3961 gtttgaaatt ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt ttcttggatt
     4021 agcacattgc tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc taaatattga
     4081 taagaaggaa agaaaacgaa ataattcccg acctttccat cagcaaaagc atgaatcgtc
     4141 tctactaatt ttgccagact gtgttccctt tttcgataag tcgtctctag gtaatttgcg
     4201 atgaaaactt gctggtttcc ttctgggaaa ggactcggca gtttgtaagc cagacttttt
     4261 tgtcccccca gtgtttcctg ataataagag agtggtgaaa aactagcaga aaaaagaata
     4321 ctgctttttc ctttatccaa cgactgctca agaaacaagc ttggatcaat acaaaactct
     4381 tttactatca gatcgcgata cgttttttcg actgttgttt cgtagtgatc atcataaaat
     4441 tcactgacct tcaaaaaatg aagcaaatca aaataataag gaagcaattg ttcttgtgat
     4501 gggtgctcag gaaattctgc cagccattct ttaatttttt cgcttaattg ataacctgcc
     4561 tttactaaag attcagccgg tgctttttga tgatgataat cccaatgatc ttcttttgct
     4621 atttctcgga tcgaatcaaa ttcttttaat agtttgttga aacggcgatg aagttttttg
     4681 aattcctttg gtatagcttc cttacttcgt ttcgtttttt cataagatag ttctgccgaa
     4741 tacatctctc gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa cagatagtcc
     4801 tcgttttttt catcttcaaa aaaacgtctt aaataaacag ttggatcaaa taaataattg
     4861 taatccccaa tgattacgtc acaccagata ctgacatcta atgatagttc gaaaggacaa
     4921 agtgtatgct ttcttgcata agtttcgatg acttcacgag tgatctgatt ctcatgattc
     4981 aaaagatccc ataaaccttc attaatccga ttgtaataac cattggcata tgggcactga
     5041 tctggattac aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt caaagtgaca
     5101 ctttttactt cagctcctgt atcttttaat gctgtcatag catcttcagc tacttgtcta
     5161 gttattgttt tagccgttag ataaaagatc ttttctccct cttcttcccc tacagctttc
     5221 aaagcgggga ataacgtaga gattgttttt cctgttccgg taggtgcttc tacaaacaat
     5281 ttctgtttcg tacggatcgt tttataagca gctaccgcta gttcacgctg acctttccta
     5341 tactcatcat gggggaattt caaagccatc aaagaggcat tgcgtacagt gcgccagttt
     5401 tcttgaaaaa caagccattc tttataatct tcagtcagct ttttaaaaaa agtttctaac
     5461 tgctctcttg tctgatgttc aacctttctc gtgatcactt cttctgccgt ctggaagtaa
     5521 gtcaattgca gatttatttc ttgtaaatca tgttcttgac tatagatata ggcatacacc
     5581 cttgcttgat gaaagaaaag ttccacttgt tctggttcta aatcttcaaa tcgtggttcg
     5641 gaggttttga tttcatctat ataataaata ccgtctcttt tgaaaatacc gtctgcacgg
     5701 ccttcaacga tcaggtaata ttcatctaat tgaactgttg tttttaaaaa gacttctttt
     5761 tcatagtttt ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc ttctaatgca
     5821 gtatggttac tttttttacg attgtcgata cttccacgtc gcaaaataaa ttctactaag
     5881 tgccgcacag atatttttga ctgtttgatc atttccacca cctctttctc tctaagatta
     5941 cctgaaaaaa cctagtttag caaaaagagc tctctttcca aacataaaaa aacaccctgc
     6001 tgattaggca gggatatagg atatagaatt tggatttttg gatgaatcta atgtaacaag
     6061 gacatattaa aatgctatga aaacttactt tccttctctt tagaatatcc aaatactttt
     6121 catatagtct gataattcag ataagtaaag agaaataagt acctgttttt gtttcctagc
     6181 aatgaatgcg ttatactaat ggatgaaaga agttttggct atcaaggagg aatgcacatg
     6241 catatcgaaa gattaaaaac aggaatgatt gaagaaaact gttatttggt ctacaacgaa
     6301 gaagcacttt tgatcattga ccctggagaa gatgcggaac gaatcaaaca agaaatcaaa
     6361 aaaacaaacc aacagccggt agcgattttg cttacccata ctcactacga tcatatcggt
     6421 gcagtggaac ctttacgcgc gtattatcaa atcccagttt atgtcagccc actagagcaa
     6481 aaatggctag gtgatccgat tttgaatctt tctggactcg gtagacatga tgatattgct
     6541 gatgttattg tctcgcctgc agaatatgaa tttgaaatga aaacttatac tttaggaaac
     6601 atgtcttttc gcgtcgtccc taccccaggg cattctatag gaagcgtcag ctttattttt
     6661 gatgattttg tcgtttctgg cgacgccctg tttagaggaa gtatcggtag aaccgatctg
     6721 tatacaggga atttagaaca attacttcat agtattcgga cccagctgtt tgtattacct
     6781 gatgaatttg cagtctatcc aggacatggc gatgcaacga cgattgaaca ggaaaaaagg
     6841 acaaatcctt ttttcaatcg ttaaaacgac ttaggaggag aataaaatga cagaaactac
     6901 actttatatc gtacgacacg gaaaaacgat gttcaataca attgaacgag tacaaggctg
     6961 gtgtgataca cccctcacaa agcaaggaca agaaggcatt cattatttag gtcgcgggtt
     7021 aaaaaatgtc gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa tcgaaactgc
     7081 ccgtattatt ctaggagaac attctaaggg aaaagaaatt ccttatttta ttgaaaaaag
     7141 gattagagaa tggtgtttcg ggtctcttga aggtggatat gacatggaaa tgtggggtgt
     7201 gatccctcgt gtattgaatt ttaaaacata tgatgaaatg tttaccacag aagtttcttt
     7261 tgaacaaatt gctaatgctg tatacaactt agataccgct gggtgggcgg aaacttatga
     7321 aacattgcga gaccgtgtat ggacaggatt tgaagatatc gcccatcact gcgaaaaaaa
     7381 tggtggtgga aatgttttag tcgtatctca tggtttgacg atcgcctttt tgcttaatct
     7441 gatcgatcct aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa caaaggtcac
     7501 ttatgcaaac ggtcgatttt ctatccacgg catcaatgat acgtcttata tcgataacgg
     7561 gaaaacacat agtcgcgact cgtttctata atggggatat tttaattttt accagagaat
     7621 cataaaaggc aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt gtacttatct
     7681 gctttatttc tcaagttttt cttaatatta aaaaacagat tgggtttctc aaaaaataac
     7741 ggtatactat aagatgtatc ttaattggga aggaaggaat ttatgttttt tgcaaatatt
     7801 atcaaagcag tgattctcgg tattattgaa ggaataacgg aatggctgcc catcagtagt
     7861 actggtcatt tgatcctagc agatgaattc atcaaactgg atgccagttc acaatttatg
     7921 tcgatgttca atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt tctttatttt
     7981 cacaagttaa acccattttc tccaagtaaa tcaacggtag aaaaaaaaga tacgtggaca
     8041 ctttggttta aagtgatcgt tgcttgcata ccagccatca tcattggttt gcctttggat
     8101 gattggttag aagcaaattt ccacaagttc ttaccagttg ccattgtttt gatcgtttac
     8161 ggtattgcct ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg ttggacttct
     8221 ctgaatgact ttacgtacca agcagctctt cttgttgggt tattccaagt cttggcactt
     8281 gttcccggaa catcccgttc tggcgcaacg atcctaggat ctatcttgat tggtgcttcg
     8341 cgttttgtcg ctacagagtt ttcatttttc ttagggattc ctgtcatgtt tggcgcaagc
     8401 ggcttgaaga tcgtcaaata tttagctgaa gggaattcat tcaaaatgga agaaactgtt
     8461 gttcttcttg taggaacatt cgtatctttt gtggtttccg tcttagctat caaattctta
     8521 ctaagctatt tgaagaaaaa cgactttacc gcttttggct ggtatcgtat tatccttggt
     8581 atcattttga ttggttattg gtttattgca atgtagaaaa aaccagaaca cttctcttat
     8641 ttgagaaatg ttctggtttt ttattttctt taataataat cattataaat aataaaaaca
     8701 acatcaagct taatcaccaa atgataacca ttatcgttct catttagctg ttattattat
     8761 caattacatt tttattatgc tatccttgtt ttgacaagga ggtctaataa cgatgactaa
     8821 tcagaaaaaa gcttttgcag caacaatcgg ctgcctttta tttatgatct tcggttttat
     8881 cttcgaccga ttctctctta ccatcacccc acttttttat atcagtgcca ttatcatcgg
     8941 tggatataaa caaacatcgg aaggcttgaa agaattgtgg aatgatcgga cactgaatgt
     9001 ggatttattg atggctttag ctgctattgg tgcctgtttg atcggtaatt actttgaagg
     9061 agcaatgttg acattcatct tttgtttaag cggtgcatta gaggaataca caactaataa
     9121 aagtcaaaaa gaaattactg ctttgatgaa tcttcaacca caaaaagccc aattattaaa
     9181 agaaaatgga caaatgcaag aagtagatgt ttctcaacta aaggtggata acttggtatt
     9241 tgtcgctaaa ggagcagctg ttccgataga tggcttttta gaaagcacac aggcaacgat
     9301 cgatgagtct gcattaagcg gtgaatctgt gcctgtagaa aaatatttag gcgatgagat
     9361 gtatgcagga acgctgaatc aaggaaatcc catcacgatt cgagtaacaa aaacgagttc
     9421 cgaaactgtc tttgctaaga ttatccaatt agtagaagaa gcgcagaata cgccaactca
     9481 aacagcttca tttatcgaac gaatcgaaaa taattatgta aaattgattt tgcttgctgt
     9541 tcctctaatg atccttctcc cccacttttt cctaggttgg agttgggatg aaagctttta
     9601 tcgaggaatg gtattattag ttgtggcttc tccttgtgcg cttgtcgcat ctgctacacc
     9661 agcttctcta gctgctcttt caaattctgc aaaaaatggg attttaatca aaggcggtat
     9721 ccatttagaa aagctttctg aattaaaagc gattgccttt gataaaacag gaacgctcac
     9781 taaaggaaaa ccagttgtaa ctgacagctt tttctttgat gaaaaggatt tggcacaaca
     9841 acttcttgtt gctatggaac aaaaaacgac gcatccactt gctcatgcga ttttgcaata
     9901 tttcgattgc acaatacctg aagaaatcca atacttacca atagaagaaa ttactggttt
     9961 tggattacaa actacctatt taggtgcgca atggaaagtt ggcaagcacg catatgatcc
    10021 tgagacgatg ataatcagca aagagattgc ggaaatgatc gaacgtttgg aaaatcaagg
    10081 caaaaccgtg atttatcttt ctaaagatca gcagttaatt gctgttttag gtctgctaga
    10141 tattccaaaa gcaaataccc aacaagtaat ttcctacttt aaatcccaaa acatccatac
    10201 aagtatgata accggcgacc attctggcac agcaaaggcc attgctgaac aagtagggat
    10261 tgaacattac tatgcaagct gtactccgga agaaaaaaca caactagtca aaaaagagaa
    10321 tgaactttac caagtaaatg cgatggttgg tgatggagtc aatgatgcgc ctgctttagc
    10381 agccgcttct ttaggtgtcg ctatggggca agggacagat gccgctatgg atatcgcaga
    10441 catcgtctta atgaaaagcg acttaaataa atttgtctac agtcatcggc tatctttaaa
    10501 aatgcggcgg atcatcaaac aaaatattat tttttcgatt cttgttattc tcttgttgat
    10561 tgcttctaac ttcctgcaat ttttaaatct acctcttgga gttgtaggac acgaaggaag
    10621 cacaatattg gttattctga atggcttgcg tttactaaga cctttacctg atctttctga
    10681 ttcttcaaaa aaatgtaagt cttgtccact atacaaagcg cggctgtctt ctgtcaaata
    10741 agcaaagtgt ttttttatga ttttgtttat tttatgaaaa aaacagctta tgaataggtc
    10801 ttgaaggatg aattcctact tttgtaactt tctaaaaaca tgttatgatt gtttcgaaga
    10861 aagtgaggaa tatatttcat ggaaagttgg ctttttttag gaattatttt acttgtagca
    10921 attttagcta aaaatcaaag tctgatgata gcggcagctt ttgttttgat tatgaagctc
    10981 ataccagcct cccgttcctt tttcccttgg cttcaaggca aaggaatcaa tattggggtg
    11041 acgatcatca cagcggctat tctaatccct atcgcaacag gagaaatcgg tctacgtgat
    11101 ctattagaag cctttaaatc ccctattggc tgggtagctg ttttttgcgg tgggctagtg
    11161 gctgtcttat cttccaaggg tgtaggattg attgcttctg atccacaaat cactgttgca
    11221 cttgttttcg gcacgatttt aggtgttgtc ttcttaagag gaatcgccgc cggaccggtc
    11281 attgcttctg gtatcactta ctgtatctta caagtatttg ctttaggaaa atagaacaaa
    11341 ttttgtctat tttaggagat tcttaacacc cccaatttac tgatccgtga tatactgaca
    11401 acgaggtgat acaatgttcg aaaaattaaa gtatttcata ggaacaatcg gctggtttct
    11461 tctagcggta cttttgattc tgggcttagc ttatctatta tttcctgatt tgatacaaaa
    11521 tgtgtttgat actgcttttt gattgaccta ttaaaacgat ataggggatg ctcctgcgat
    11581 gcaggggctt tttttaatca atgttttgta ataatttagg ccgtgccaat gagacttcct
    11641 atagaattcg atgctctttt ttctttttgg ctcttctgct cttttttgtt atactagatt
    11701 acagtggaca aaagaaagaa tgatcgcatc cacttcaacg aatatcaaaa tggaagaatt
    11761 agtgcctgtt ttcaggttac tttgaaaaga aaggaagacg cttgtggaaa cttttgaagg
    11821 tcttttaatt gatacgtacg ttttacaaga acagccaatg atgctgactg ggatcaactt
    11881 gcagcttgag gaccgtgtga tcaatggcct gcttaaagat cgctatttgg caaaatatat
    11941 cttacgtttg cccctaaata aatatgtgct ggaagtaagt ggcaaatgga accgtcggaa
    12001 ccagttggtc ataaaaaaaa tgttgataaa aaatatcgat gaatacatta aaataatcgg
    12061 tacaccagaa tagcctttac tgatcaaagg atactggtta taaaataact gcagtttctt
    12121 aatagaaaaa caaagaacaa cagggaatcg tatccctttt caaaaaaaac tttgcaaacg
    12181 gttctctttg ttatataata acttagtacc aaacattccc attcaaactt tgtttaaata
    12241 gataaataaa aatctagttt ggtttgacgc tattgcctcc cacaatagcg tctttttgat
    12301 ttttttcagc ttgtactatc cgtttttact gaaaaaaaat cctaataaaa gactgttaag
    12361 gaaaaaaact tgtcatgttc atattgcaaa gcacgctgcc tttaggtata ataatgaatg
    12421 ttgaattatg cggtaacacg cgtaagaact taagttatcg gaggtgaaac taatgccaaa
    12481 cattgaatct gcaatcaaac gtgttcgtac aagcgaaaac gctaatgtga aaaattcatc
    12541 tcaaacaagc gctatgcgta ctgcaatcaa aaaatttgaa gatgctgtag cttcaggtgc
    12601 tgacaacgta gatgcgttat ataaagaagc tgttaaagct attgatatgg ctgaatcaaa
    12661 aggtctaatc cacaaaaaca aagctaaccg cgacaaatct cgtttaagca aaaaaatcgc
    12721 taaataaaag aatcggcttc ggccgatttt tttatttgtc aaaaaaagga caaaaacaaa
    12781 cgaccttccc gcatgctttg ccctcgtctt tcttcttatc gatttcttaa caactgccct
    12841 gtgcgcaaaa cgaatagttg aaaacttaat tctttttcga tttgtcccgt tttgatttga
    12901 taatccatct caactaattg atcaaacaac tgtaccagca aatgttcatc gaattttttt
    12961 acttcctgca tggcaagttt tacacgatat ggatggattt tcaatgtttc agcaatattt
    13021 gcctgctgat aaccaatctt tacaagaaat tttgtctgta aaaataaacg taattgagaa
    13081 agcaaaatcg cattaatttt gattgtttct tccccttgca taactaaatc ttcgtataaa
    13141 cgcaaagctt gttccgtctt cccttttaaa atatattggg tcatgtcaaa aatattatgt
    13201 tctaacgttt taggaactaa ttcttctact tctcttaccg tgatttgctg ttgattttgt
    13261 ccataaagga ccaatttctg taactctctc atcattttgg ataaatctaa gtcggtcaac
    13321 ctcaagaata aatcaattgc acttcgttcc attttgattg aggaattctc taaggtattc
    13381 atcagatatt gacgtacttc tttttcattc aactgttgga cattgatgat aattgctgtt
    13441 tttttcaatt gtttcgttac ttttttccgt tcatccaact tttcatatgg cgcaaagaat
    13501 actaatacag tcgattctaa tggttctttc aaataagccg tcaataaatc agtattttgt
    13561 tcgattccat tgtttacttt ttcacctgtt aaaaaataag gattctctac gaatactaac
    13621 cgttgatctc cgaaaaacgg caacgtctct gcttcagcta ctacctcatc taaacttgca
    13681 ttttccatgt caaaacttaa aaaatttaag tcatcttcgc cctctaattg gatttttttg
    13741 acaatttctg tccgaacttg atcttggaga aacttttctg ttcctaaaat caagtaacaa
    13801 gatgcaagtt tttctttacg tattttttgt aactctgctt gtatactcaa attactcacc
    13861 actttcaaaa attatcctac catgagcaga tgagaattac tagtctatca ttgtttccac
    13921 tagcccatga ggatgaaaaa ctggatgcca gcgataggtg atcattcctt gttgatccgt
    13981 tcgccatgtt tttatctgat tttcttctag tgtctccaat acttcgctgt gcgggtgacc
    14041 ataacgatta tttacgccgc aggatataat cccatgtttt gcttcaatat gggaaagaaa
    14101 atctgtgtta cttgacgtac ggctaccgtg atgacctaat ttgactacat cggctttcag
    14161 attaggataa atcaaaagca attgtttttc cattgcttga ttcaaatctc ctaaaaataa
    14221 aaagcgccta ttgttatatt ccatccatag accaatcgaa tcttcatttc ttccctctcc
    14281 cttttcaaaa ggagtcaata cataaagatc ataataaccg ctgatcctgt ccccatgttt
    14341 gatttcaatg atctcagtac catttttttg agcttcagat aaatgccttt gtaatggctc
    14401 atttcgtcca gcgccccaac cgatataaat ttttttgata gtaaaatgct gagataacgt
    14461 gggtaattct cccacatgat ccgtatcgtc gtgtgtcaag atgagtttat cgattacact
    14521 tatcccctga ccttttagat atggaacgat atttctttca gcaggaggac gtatcatacc
    14581 ttgcgcccac ttttctcttt caaagtgtaa ctgacctcca gtatcgatca aaatcgtttc
    14641 ttttcgaaaa ggactttgca aaaagatact gtctccttga ccgacatcga taaaagcgat
    14701 cgttgaagaa aagcgtaaaa atggcaaagt caaaaaaata aaagaaaaaa gaccaacata
    14761 aaacaagtgt ttcacaggtt gttctttcaa cctatccata caaatcagta atccgaaaat
    14821 atacatccca gatagcacaa ggggcaaacg tcctataatg agagacgacc ctgaagaaaa
    14881 atcgattatt cgctcaaata agattaatcc ctcttctatc agcaaaagta aagtttccgg
    14941 cagccagcga ttgaagagtg tgagaaataa cacaccagga agaaaaagaa acttgaatac
    15001 cggtgcaatc agtaatgtaa aaagactacc taaaataggc cactcataaa aatgccatac
    15061 aatcaacggc atcgtgatca tagtaaacag gacgtgagag gccgtttttt tcaaccaatc
    15121 tccatcaaaa gaaatcagga taaacaaaaa actcatgaaa aaactaagct gtccacccgc
    15181 atgaaaaaga aatcgaggat caaatagtaa aagaagccaa aagacaactg caaataagtc
    15241 catggtggaa taatgcttat tcagcaattt gactagcaat cgcaataaaa acagcaaact
    15301 agcacgcaaa acagaaatag aacttcctgc aatagaaaca taacagatag ttagtagact
    15361 caacggaata atactgacgt gtagtggcca tccgatacgt cgaaacaaat aatacagcca
    15421 ccctaaataa aattggatgt gtagacccga tagactgaac agatgcagca aaccactctc
    15481 cctaaaaatt tcttctggtt ctctgaatcc tgcatcttta tagccccata ataaagaatt
    15541 aatatataca cttgtttttt gcggaaattc agattggaca aaggagataa aagatcctct
    15601 ttttctctga agccagtttc gccagtgatt ttgcatggtg atctgttcaa tcttttgaat
    15661 ttggaaccgt ccagaaattc tatggttcct gaagtaagct tcttggtcaa aattataaag
    15721 attccgcttt tttgctggag agatgaactc gccttcagca gtcaattcta ttccctttcc
    15781 tcggaagtta agccatgttt tcaattctgc ttctgtgttt acttgataag aaagctggag
    15841 cttttgttta ttctctgata accttccttc cattgtcaaa aaattaccat ttgcacgaat
    15901 cgtatctgta aatacagtaa tttgcgcaga tgttgttttt tcctctaaaa atgaatactc
    15961 ttgtagccta taagaaatgg ataccgttat gctacacaaa atacacaagt acgcgataaa
    16021 acgtccttct tttagcgaaa accataaaaa aattccggca agaagcaaac gataacccca
    16081 attgtcagtg taatacaaca aacagataat catcgacgta attgccggaa aaatcaaccg
    16141 atttcgaaca tgcctttcta aaacgctaga catgttttcc tgttaattca ttttgaagtt
    16201 ttgaaaaatg ctgtgagtct aaagcaactt ggtgtacaga tacacctaat tgttcgatca
    16261 acgcttgggc ataaggatca tttcgatagt catggagata attgatccct ttcacaccag
    16321 cttgcaacaa agcctttgta caggccaaac atggaaaatg tgtgacatag atttctgaac
    16381 cttccgtcgg gataccaagt tttgcacact gtaggagtgc attcatctct gcatggatcg
    16441 ttcgcaagca gtgtccatcc acgacgtaac acccttcatc tatgcagtgg acatctccac
    16501 tgactgctcc attatagccg ccagcaatga tccttttttc tcgaacgagt gtcgctccta
    16561 cttctaaacg tgtacacgta ctacgtaaag ataacagtac tgcctgagcc ataaagtact
    16621 ggtcccaagg aatgcgttca ttatgtgact tatcagtaac tttttcttta ttcgtatcgc
    16681 atgcttttga agatttttct aacatgtttt atattcctcc gttttttcaa gttgattaaa
    16741 aagtactcat tgcttaaaca aaatattctt taaatataac gaatcatcct gtctgacaaa
    16801 agatcctacc cttcactgcc gaaatgaagt ccgtcatcca ttagcccatt ataaaaattc
    16861 ttcgaatcca tttgttcaag aaattccgta taaaaatcga tacttcacaa tcatggcgtt
    16921 ttgctacttc tgtgagaaca gagatatatt ctttgatcaa cttgttattc cgatattttt
    16981 gttttgtctc atcgactgct ggtggcgtga taaagaaagc atgatcagaa ctatagtctt
    17041 ttttcagctg aactatcatc cactgcagat tttttggtac gtttctttag aaagttgttt
    17101 atgctctgct agatcattcg tcccaatgag aataaatact ttatctgctg tatagctaag
    17161 tacatctttg gcaaagcgtt ctcgtagagc ttgactgtta ttgccgctga cagccgtatt
    17221 cataatatga atcccagata ttttattttg gagatagaca ttcagcattg gtttttttaa
    17281 tgtttctttg cgaacaatca agctatttcc tatccaaaaa tactacctcc tcgtcctgcc
    17341 aagctcttag aattggatta ttcttagtat ccattagcgt ccatttattg ctcgtgccag
    17401 tcgattttga atcattctat ttaattgtaa cgaagttttt tagtttttca actgtctttt
    17461 caccgatacc agaaatctcc tgcaggtctt ccactttctg gaaggatccg ttttcttctc
    17521 taaatcgaat gatttcttgc gctttgactg atccgatccc aggaatctgc tgaagttctg
    17581 atggagtggc tgtattgata tttattttgg gaggctcgga atctgctgta ggctgttttc
    17641 cttccgagga tgccaactga tcaagatgtt ctggcagctc ttcacctaca gcagggatat
    17701 ataggacttg ctgatcttct atcttcgcag aaaagttgat tcgatcgcta tccgcttctt
    17761 ctaataatcc acctgcacgc tttagtacat catagacccg ttcttctgaa gaaaaggaat
    17821 agataccagg ctccttcaca cttcctttga tatcggtgta catgaccttt ggacgagcat
    17881 cctcttctac atcgtttcct tcagctgatg gatcttctcc actggaacta aacgaagaaa
    17941 gtatttcttt tgcagcagtc tcttctgttt ctctataaaa tagcaaattt gataataaaa
    18001 aaccacttgc gatacaaaag ataagaacta tcatagacat ccaaatgatc aatttgggtg
    18061 aagctagtat tttcttgctg attttttcta tcattgttct tttttcaaag aatgaccagt
    18121 aaaaatcatt cattactcct ttcatttaa
//
LOCUS       DAZUMI010000055        17956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000055 DAZUMI010000000
VERSION     DAZUMI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.055"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACG81C_001607"
     CDS             133..636
                     /locus_tag="ACG81C_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5064773.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACG81C_001608"
     CDS             763..1800
                     /locus_tag="ACG81C_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFS5064774.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACG81C_001609"
     CDS             1815..3512
                     /locus_tag="ACG81C_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFS5064775.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACG81C_001610"
     CDS             3505..3966
                     /locus_tag="ACG81C_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFS5064776.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACG81C_001611"
     CDS             3979..4839
                     /locus_tag="ACG81C_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064777.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACG81C_001612"
     CDS             4877..5266
                     /locus_tag="ACG81C_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5064778.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACG81C_001613"
     CDS             5253..7700
                     /locus_tag="ACG81C_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5064779.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACG81C_001614"
     CDS             7705..9813
                     /locus_tag="ACG81C_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5064780.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACG81C_001615"
     CDS             9810..10814
                     /locus_tag="ACG81C_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5064781.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACG81C_001616"
     CDS             10833..11744
                     /locus_tag="ACG81C_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5064782.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACG81C_001617"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACG81C_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACG81C_001618"
     CDS             complement(12406..12621)
                     /locus_tag="ACG81C_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064783.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACG81C_001619"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACG81C_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACG81C_001620"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACG81C_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACG81C_001621"
     CDS             complement(15379..16050)
                     /locus_tag="ACG81C_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS5064784.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACG81C_001622"
     CDS             complement(16399..17850)
                     /locus_tag="ACG81C_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5064785.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5848 a         3016 c         3472 g         5620 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagt
//
LOCUS       DAZUMI010000056        17582 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000056 DAZUMI010000000
VERSION     DAZUMI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.056"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..980)
                     /locus_tag="ACG81C_001623"
     CDS             complement(90..980)
                     /locus_tag="ACG81C_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFS5064786.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            complement(995..1618)
                     /locus_tag="ACG81C_001624"
     CDS             complement(995..1618)
                     /locus_tag="ACG81C_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS5064787.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            complement(1745..2062)
                     /locus_tag="ACG81C_001625"
     CDS             complement(1745..2062)
                     /locus_tag="ACG81C_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064788.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            complement(2064..3116)
                     /locus_tag="ACG81C_001626"
     CDS             complement(2064..3116)
                     /locus_tag="ACG81C_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS5064789.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            complement(3034..4563)
                     /locus_tag="ACG81C_001627"
     CDS             complement(3034..4563)
                     /locus_tag="ACG81C_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5064790.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            complement(4575..5990)
                     /locus_tag="ACG81C_001628"
     CDS             complement(4575..5990)
                     /locus_tag="ACG81C_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5064791.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(5983..6816)
                     /gene="terS"
                     /locus_tag="ACG81C_001629"
     CDS             complement(5983..6816)
                     /gene="terS"
                     /locus_tag="ACG81C_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFS5064792.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(6975..7205)
                     /locus_tag="ACG81C_001630"
     CDS             complement(6975..7205)
                     /locus_tag="ACG81C_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064793.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(7952..8419)
                     /locus_tag="ACG81C_001631"
     CDS             complement(7952..8419)
                     /locus_tag="ACG81C_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFS5064794.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(8494..9033)
                     /locus_tag="ACG81C_001632"
     CDS             complement(8494..9033)
                     /locus_tag="ACG81C_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5064795.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            complement(8964..9257)
                     /locus_tag="ACG81C_001633"
     CDS             complement(8964..9257)
                     /locus_tag="ACG81C_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064796.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(9288..9482)
                     /locus_tag="ACG81C_001634"
     CDS             complement(9288..9482)
                     /locus_tag="ACG81C_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064797.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(9588..10040)
                     /locus_tag="ACG81C_001635"
     CDS             complement(9588..10040)
                     /locus_tag="ACG81C_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064798.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(10066..10527)
                     /locus_tag="ACG81C_001636"
     CDS             complement(10066..10527)
                     /locus_tag="ACG81C_001636"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5064799.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(10528..10662)
                     /locus_tag="ACG81C_001637"
     CDS             complement(10528..10662)
                     /locus_tag="ACG81C_001637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064800.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            complement(10728..11579)
                     /locus_tag="ACG81C_001638"
     CDS             complement(10728..11579)
                     /locus_tag="ACG81C_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5064801.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(11594..12394)
                     /locus_tag="ACG81C_001639"
     CDS             complement(11594..12394)
                     /locus_tag="ACG81C_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS5064802.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(12437..13327)
                     /locus_tag="ACG81C_001640"
     CDS             complement(12437..13327)
                     /locus_tag="ACG81C_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFS5064803.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(13329..14270)
                     /locus_tag="ACG81C_001641"
     CDS             complement(13329..14270)
                     /locus_tag="ACG81C_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFS5064804.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(14503..14838)
                     /locus_tag="ACG81C_001642"
     CDS             complement(14503..14838)
                     /locus_tag="ACG81C_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064805.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(14897..15025)
                     /locus_tag="ACG81C_001643"
     CDS             complement(14897..15025)
                     /locus_tag="ACG81C_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064806.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            complement(15164..15352)
                     /locus_tag="ACG81C_001644"
     CDS             complement(15164..15352)
                     /locus_tag="ACG81C_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064807.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            15430..15654
                     /locus_tag="ACG81C_001645"
     CDS             15430..15654
                     /locus_tag="ACG81C_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5064808.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(15651..15806)
                     /locus_tag="ACG81C_001646"
     CDS             complement(15651..15806)
                     /locus_tag="ACG81C_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064809.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(15946..16158)
                     /locus_tag="ACG81C_001647"
     CDS             complement(15946..16158)
                     /locus_tag="ACG81C_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064810.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(16161..16373)
                     /locus_tag="ACG81C_001648"
     CDS             complement(16161..16373)
                     /locus_tag="ACG81C_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFS5064811.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            16648..17058
                     /locus_tag="ACG81C_001649"
     CDS             16648..17058
                     /locus_tag="ACG81C_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5064812.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            17071..17523
                     /locus_tag="ACG81C_001650"
     CDS             17071..17523
                     /locus_tag="ACG81C_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5064813.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
BASE COUNT         4952 a         3631 c         2677 g         6322 t
ORIGIN      
        1 ctctatattt cacgacttca tcttttttag tttcatcttt ttttaccact gtaattcctc
       61 ctatcaaaaa agggagtact aactcccttt tattcagact ttaaatttaa aattgcccca
      121 gagtttgaag cattgtattc aagagagtat tcacctacaa gaccgatacg tctagaatcc
      181 gttgtttttg caagttcttc tgctctccat tcacgtaatg gacgtagttt cacataatta
      241 gtatcgattg cggcaattgt tccttttggc aatgatggtt ctagtagagc aatacctgta
      301 ccgtaattag acacgatatt tccaatttgc aatccaaaag ttactctttc cccgaactga
      361 acaatttttg ttgattttcc atctaactca tcagtcatta actcctgcat atctggagaa
      421 atcaagcata gtttttctcc catataacct ttttcataca tcaatttaaa taaagtatcg
      481 atatcttttc ttgttactgc acccgcagtg gctgtttctg ccttatttgc tgaattgatc
      541 aagttcaaaa tcccattcat tcgacgacct ttagaaccat tttcatcagc ttttacacca
      601 gtaatcaatt ttcggttcaa atcaattttc atttccatac cacgtagtgc tacttgatta
      661 gttaattcat ttccgactcc attcacatta atagcatcta atgtaccaga tacagaggtt
      721 gattttctaa aaatttcagt atagttgtta aaccaagttc gacctgattc agcatctgga
      781 tattctccgc cctcaagttt ttcagaagaa tcatcgttgt tgatatcata ttcacgccat
      841 ttaatttctg tcgagtttgc tggctctgtt tttccagcac caagtaaata gcttaaaaaa
      901 ggtgtatttg gtacttgcat agcattaatc gctggtgaaa tatccaaata ctctaaatta
      961 tttaaagatg tttttttcat aatttttcac tctcctagtt aaattgttga agaatttgtc
     1021 ctaattgttc ttctggattt gttaatgttt cattgttctg ctgaacctgg ttattcgatt
     1081 gttgagttcc accaaatgca gacttcattt gtagttcttt taatgcatct gcatgtttct
     1141 cattaattgc attcaaaaca cttgtaaaac cttcaacagc attcttagtg aaatctgtat
     1201 ccgaactaac aagattattt agcataaatt gagaaataga ctcttttaac tctccatcta
     1261 gttctaatcc agcaatttgt tctgcaacaa atgctttatt atcacttgtc acacgcaacg
     1321 ctttttctgc ttcaaattca gcttgtagtt tttccagttg aatttgctca ggtgacttgt
     1381 ttttcttaga ttcctcatat tccttgatgg tatcctgttt aattttatct aagttatttt
     1441 gtttccaagc ttccaattgt ttgtcggcaa cactttgaga ttgtgactga ataaattttt
     1501 gagcttcttc attggattcc acaaaagatt tgaagtcatc aaaagaaaac ttttgatcgt
     1561 caccttcggc aaagtattgc aaattcattg gcattaatga tttgtgtttc atgctcattc
     1621 tcctttcgcc ccacgattcg ttttcacgcc ccgcattgct ttgagtttta atagttgcgc
     1681 cccaccattc aaccaagccc agtattgcgc tagtttaacg tcatttcgga caaaataaaa
     1741 aagcctaatt actagacttt ccttctttgt agtcagttct aacaacaata catcccatct
     1801 tttcatacca atcaactgtt tcttttaaat ttggtaatga atgagataat aattgaatcg
     1861 ttaaattaac ggtattctta tcaactggag tattatcttg ataattaaca gtttgatcgc
     1921 caataaccac gtaggcataa ttgccattcc aatggtctct caagccatta ggatggttct
     1981 cagactcaat agctgttttg taggcttctg caatatcagc atttacatta attgttagta
     2041 ctgcttcaaa aaaatctttc attctacttc ctccgtttca aattaattcc aaaagaatca
     2101 tgtgcaaact catctaataa atcgccaaat aaacggtcat attcttcatc aatatccttt
     2161 cctaattttg gtaaattagg aacatccgta catctacaac gactatggaa aggtgctcga
     2221 ttttcaccta tgacagcatc tttcagttta tagggattct tacttgcctt tcctccacaa
     2281 atccggcaaa ctctctcatc ttttgcagtc aatacgttgt attcctcaat acctgtttct
     2341 aaatatgatt tttcaatacc atcttgtgca aattttgcat actccgttcg aacgagattt
     2401 tctatcgctt tgttatactt tgattcttct aacttaaaca tgtcacaaat ttctgagtct
     2461 aatctcatgg tgtttaatgc gtgtataacg ccttctccac tagcaacact ttttacaata
     2521 gcattagaga gtttctgttc aagcgatgaa atatttcccc aaagtcttct tgaaaatgtc
     2581 tttcctgacc atggatagtt caaataatgt ttaatttcat tttctggtaa ataatttggt
     2641 atatcaacgt ttaaaagttg aacaaaaaca ttagcattag aagaatatgt tctttgaaga
     2701 atttcctcta agcgatcaga aaaatattga ttcacatctg catccatcgc atattctgca
     2761 agaattttaa atacatctga tcgtatttgt aataagcgat tgacttttgc atagtcaaat
     2821 ggtggaaaaa attcatcgat aaactctttg tattttgcat catacatttt taatgactta
     2881 taattttttt ccacatattc acgatatttt ttctgattac ttgtactgta aaactccatc
     2941 atttctggat aagtgatatt gtgtaagtca gcttgtgata atagttttgc ttgtatctct
     3001 ttcagtgctt caggaaatac actagttaat tttttaagtg tctgattttc ttgtttgagc
     3061 cttgcttgat cctccagttc tcgacgtttg gtccaatact tcgtttctat cgtcactatt
     3121 gccacctccg ccaaaattgt attcactatc tggataagca cttccacttt ccaattccat
     3181 catttcattt tcataatcaa catctgttac aaatggaatt tggctctgaa tagttcgttt
     3241 ggatacgtat ggtgcaagtt taggtaaagc ttcagctaaa taacttaaat cagttggcaa
     3301 agatctagag aaagtaaaaa taattttttc aggctcaatt tctactttgt catgaaactt
     3361 aagaaaagca gcaattgttt ctgcgcattc tttcaatcct tctctaaaat attgctcttt
     3421 agtatttgtt ttcgcttcta ggctaatgat ttgccacttg cgagcttctc cagaactgtt
     3481 tgatttaaac acctcatcgt taaaatcgat tgctttacaa atggtgtaaa actgtttttt
     3541 caatagatcc atatgatatt cattgaaatc ttttgctaaa ttttttgtga catattcagc
     3601 ttttgcagta ggggctttta agttgataat ccctagctga ttcatcattt ctttagcagt
     3661 gcctttgctc attgttgtgc cagtaacaag catgtacgca agcttaaatt gttcgatttc
     3721 gttttgttga tcagacaaag cacgatcaat tgcatctcca atttcctcag ctacttcaaa
     3781 atcacaataa cgattcgtat tatttttaaa ttctgccaag gtaatcgtcc ccaaagggtt
     3841 atcggtctca tcaatttttt tgaacgttcc gcttgtaaca aaattcaaat caccatagca
     3901 agcatatgtc agaattttat ttcttgtcat aactttcatt tcttgaaaaa attttttctg
     3961 gtaagagtca tacttttctt tgatatagat tccggcattt ccatatcttt ctgctctcca
     4021 aggttcaata ttacttgctc ttaacttcca accatcatct tcttccacag gctctagcaa
     4081 tcggaaagct actccacaag ctccttgaaa agttgcagtt tcagaatcaa gcattgcgaa
     4141 tctcatggaa tcaagattgc ttgttaattt gttaaaagct tctggaactg tcggaagttc
     4201 tatatcattt tttaagaatt tatcttttaa tctttgaatg agtgttcttt tttgttccga
     4261 tacatcgtaa tcccatttga taggtatacc agtaaaatga ttgactgctt ggtctacgac
     4321 aattgaatac ataccagcat gaagtttatt attaactttt acaatatcag tgttaggctt
     4381 tggtctatta tctatttcat tcttttcgct agtataagct agatattttc tttcgcgatc
     4441 agcaaaaaat ggtttcatat cttctataaa accattcgga tcaaacattc catcttcaat
     4501 ttgtgttgca tatttaacac gtagcttctt atatctctct aaagataata ctgtactaat
     4561 caaaagctca cctcctaaaa ttgaataaat tgatagtgac ttggtggctc ataaaacgct
     4621 aaagccagtg cgtcagcaat atcgggacta ccaatatttc gctttttcat atcatcttta
     4681 ctttctaaac gtatacgact tctacttgtc attttgaatt tgcgagtact taattctttg
     4741 attaacgaac tatcagaagg caattcaata acaggctgtt ctccgttaag atttgctgtc
     4801 atattttctt ctaacatttc cttgatgttt ccccatagtt gagtacctaa attatcgtaa
     4861 aaatcgtctt ctgatgttga accgttattc actccaaaca cctcaaaagg ataatgtttg
     4921 tcttctataa gttcttctag gcggtcggtt acaccacctc cgacaccagt gtcatcgact
     4981 ttaatcatca ctttatcaat actcggatat tgactcatta gattcttggc catgttgatg
     5041 acatatcctg ttgtttccat ggtgctacgt tttgaatact tctcatactc caatgccctg
     5101 gtagcaattc taggaaagag aatcgtagaa tcatcaccat atcgagctac gtcgacacca
     5161 atatgagcaa cctttgtttt attgactaaa gaatcactaa tttgtttttc tgtagccaat
     5221 tcaactgttt caagactgat aaatgaatcc aacgcacctt tcggaaattc tccaaaaata
     5281 cggacacgag caacatcact ttcttttcca tattttttaa gaatcatttc tatattgtct
     5341 ttgtttgtac gtttactatc atagcttgat actttatgaa ctctgtattt atcacggtct
     5401 gaattatggg aatcataaaa gacaccttca atattattgg ggtttccaca catcaacagc
     5461 ttattatcaa aacctgatag cgtaccaaga atagcttcca taattggatc agacacacca
     5521 gaagcttcat ctaccacaat caacatatgg tcctcgtgaa aaccttgcat actttctggt
     5581 ttagtcgctg ttctagccgt agcaaaccaa cgctctgaat caccaaccat ataaattttg
     5641 gtctttgtcc acttcagtaa gtttttgatc aagctatcat ttaaccattt agctacctct
     5701 gcccaaagta catcgtaaag ttgcttcatt gtcggagctg ttgctattac tttagcgtag
     5761 ggtcgacacg ttaagaacca aagtattgct cctgcttcta atgctgtttt tccaactcct
     5821 tgaccagaac gaacagaaac ctttgaaaat tcagctaaat catttaaaac attttcttgc
     5881 cattcatcag gattcaaatg caaaatatct tggcaaaaag ctactggttt atcatagtaa
     5941 taatcaatgg cagaaccaat atcagcaaaa ggaataaatt cattattcat tttctttcac
     6001 cgcccgtttg tttgcggcat ttaaaactgc ctgtttccat tcttctattt cttcacctga
     6061 attatttccg ccaatttcat ttgtctcagc ttggatctta tcagtctgag ctttaattaa
     6121 atcagttctg tattcattca tataaagttc attcatttgc ttgatggctt tagctaactg
     6181 gttgctaatc cgagtcaatg aatcttcaat tgaaagaata tcatcaattt tgcgatgttt
     6241 ttttctgcta atctgcacat cttgcataac ctcacgcttg atttctagct ttttaccatt
     6301 tttttcaatc ggagttttaa tctttcttag ctgctgcaat cgttcgactt cttcatcatt
     6361 tagtccagct tcagcttgtt ggattctttt catcattcga agttgtctta ttttaagaag
     6421 tcgtatttct tcggacaaag caaaagaagg atcatcattc aaactagaat agatgtcctt
     6481 ctcattatcg gttaacgtgt caaaaaatat tgtttcatac tcacctgttt tcaaagcgtt
     6541 tttattaccc actggcggag aagctcggct atttcctttg ttacctttag cattttgatt
     6601 accaatagga gcgccacctt gattagtaac gttacttttg ccattggtaa cgttactaat
     6661 caattcagcg ctccatttgt cttgcgattt ccattttcta acctgagaat ctgaaacatt
     6721 taattcagat gcaatttcct ttaactgctt ttctccgttg gattctaacc aaattctttt
     6781 ggcttcatca cgtcttggat cacgttgtct tgccattcaa tacacaccac ctcacattct
     6841 gtttaggttg agttttgttt tctaattttc aatatctttt agcattaaat cagcttcaat
     6901 caatatcttt aaatcggaaa ctttgtctaa cttgatttgt cctgattgga gatttttaag
     6961 ccatttcttg cattctaatc cctcaaattc ttgttaatat tattttgaat gtttggttca
     7021 tcatagaaac catgaccgca ataaacgagc ttgcacgcat caatctcaca tggttctgct
     7081 tctctcccca tttcgacaat agagtacttc cctttgattt gtacagaacg cacaacacgc
     7141 actgaacaat catcaatggt tcgaggatat tcattagtta gcgatacata ccagtagttt
     7201 ctcattatgt agcctccttt atgtaaagaa aaagaccact caaagagtga tctaatatgt
     7261 aatgcacagg cagggacgtt tccgatcctg tgcttgagac atttgacgat tcatttgtac
     7321 cgaatcccaa atctcaatct aacctaacca gttatgtaat agcaacctac acgatgcaca
     7381 aaacgcgtac gaaattgcgc acccctatat ttttaaaccg ccgatgcctc ggttgccaaa
     7441 gtcactggca aggaatcgaa ccttgcatgg ttgccgaagc attgacctag cacacatgct
     7501 tagcgtctac cctttccgcc acagtgacac tataaaatta ttcttggctg ctactatttt
     7561 ttattttgcc catttttaaa tccaatcata tatacattaa gacaaagcgc aaaaattgaa
     7621 attattaatg gaatcatttc tcttcactta cctttagtta tcgtgtgaat aattaaaaaa
     7681 acaatagaca gcaacggatg atagataata agaacaattt agaaggagtt gaaattcaca
     7741 tccttattct taatatttcc gttgctgcct atcgaagctt aattaaacga tgagggagat
     7801 ttcctccctt acattttatt ttgtcgatcc tgtttcctaa tttttcgaca ctatcataat
     7861 acaacgttga ataggtaagt gattggtata aaaaaggtat aaaatggaaa ccaaattggt
     7921 aataaaaggg tataaaaagt gtaaaaactg gctacttgaa agcaaccagt tctaacgatg
     7981 aagcaaattg gatgataatt ctgtttgatt ctactttaac cgattcttcg ctagtattat
     8041 tcctttgagc agttacataa atgggcagac cattgatata acgatcatag aatatcttct
     8101 tgcgcctttc agtcacatca ggcttatgcg gatgctgtat cgctgaatag cctcgaacaa
     8161 acaatttatg cagatactca aattcttcct gtgcttcttc tttatcgatc agcattcttt
     8221 ctgcttcaaa tatatgatca gctgtagaag gtggaaccaa ggaataagat gctgtcactt
     8281 ttggttctcg atgttgccct actctacatc tagctgatag atatgctgaa agaaacacag
     8341 cgacattatg ttttgtgcgt tccatatcaa catctttcgc gcttggtgtc tcatatttct
     8401 ttacatcgaa aagtaccatc ctctgattcc cccaattatg atataatact tatgtcggaa
     8461 atattattca tagtcggagg aatccgactt tttttatttt gtacgtgaaa tgagttctcc
     8521 tgatttatac gcctcagcaa attcaaccaa agctactgcc ttcattctct cgatagttct
     8581 atcggaatat cctaattttt ctcctatttg atacattgac atcttattag gaaaacaata
     8641 actataataa agtacctgga aatggattct acttagccgt gttaatgctt cgataaccga
     8701 atctcgagta cttatcgctt caatgatttc ttcgttttga ctgtcaattg tgaatttcaa
     8761 atttttggta acagtcatca cggaaaagtc tattttcact ggtccgacca aacgttctaa
     8821 atttctgtat ctttttagta atcgtctagc atttttcctt gtttgcttaa tatctatatt
     8881 ctctaaaatg ttcacaattg cctcttctcc ttccagttgt ggaataacgc tcattgttgt
     8941 aggatagttg taaaaaagca aaattaagat tttataactt ccatgtccac caatctcacc
     9001 actgctaaat tctctttgct tttcgctaac cacttatcac attccatcgt gttttcaatg
     9061 cgaatgattg ctgagtgatt atagacgtgt tctacatatc cacgaaatgg atagatgaat
     9121 ccttctgctt cgcagcgaac catgtcaccg actttgaatt ttggtttctt acgtgtttta
     9181 ggattctttg tcggcatatc tagcattaaa ccgccgatgc cgtggccgct ggagtaaaat
     9241 ccgtctttta gtttcattcc gcttcctcct ctttagctgt caattactta ctcgcaagtc
     9301 actaaaatgg ctacttcatc gataaagttt ccgtcttcgt cttccattcc ttgctgaatg
     9361 ctaatgtcgg taatctgctt aaaaaattcg ttgtcatagc cttgtgcgtt tatcactact
     9421 tcgacgtctc cgaaaacctc tttgagacta ttaattttat ttactacttc cgatgcgtcc
     9481 atgactacac ccctttccag ttaaaaatct ctgtcgttaa tagactactg ttcaaagtat
     9541 tcatcttcac tcatccttct gcctcctctt tagttgtcat tagtggacta aatactacct
     9601 gtttgaaaac cataatgtgc agtttcaacg ccgtcataat cacttatttc ccactcagcc
     9661 ccatcgggta tatcaacgac tttaagactt gcatatggtc cactggcctc acttcctagt
     9721 ttttcgacaa cagcaacgag gtccttatca cctctgtttg aatcacttga aaaactggaa
     9781 tcatcagcaa ttccgtacag tcggtcaatt tcgctataaa ttcctgtaac tttgtcgatt
     9841 ttcgggtctt ttttgaaata gtagataaaa tccatcgaac ctagcttttg gcctgtgtat
     9901 ctcgtgtatt catcatctga gtcatagcta aagttattga tgtatgggaa tacttcaata
     9961 ttttttcttt taaaaatttc catttttgct tcatggctca acccaaagcc accaaaacat
    10021 ttatttaata caatcttcat tacttttcct cctttgtttc ctccgttagc tgaatttttt
    10081 cataaacaca agccaatgtg ttttgcttcg tttattacca aacaatggct gttctccaat
    10141 agcctctaac acatcagaaa gtggtatctg ttcctcgttc cacttaaaaa ttaatgttcc
    10201 tgaaggtcgt aaaactcgca tacactctgc aaaaccttgc ctgatgtctt ctggccaaag
    10261 ttcgtctaat tttccatatt ttttggcaag ccatgagttt tcgccgacat gtcttaagtg
    10321 tggcgggtca aatacaacca tatcgaacac atcatcttca aaaggcatat cgcgaaagtc
    10381 tgcgaccaca tctggatcaa cattgatcac atgacctgtt gataattctt catactgctg
    10441 acgacaatcc ataaatgtta cgtcagaatt gttcttatca aaccagaaca atctactacc
    10501 gcaacatgca tctaatattt ttggcaatta ctctacctcc aataactctg gattctcgta
    10561 gacgttacct aaaacagtga gaaatctttc tgtatcccga taaataatta gcttacttat
    10621 cttccgaacc attcgactct cttgttgaga taattcgtcc atgtcacgat cacctataat
    10681 ttgcaacatt atctgacgac agattctttt ttcttgttta gtcaaattca tgcgatcgct
    10741 cctttgactg gttttatgcg cttgtctgct gtttgagaaa ataccatcgt aaatccatca
    10801 gagttgacaa acattcttga tattgttctc gtaccgtaag cttttttcag ttcttcacca
    10861 agcaagttcg ttgtgataat cgttgctagg ttctgtcgtg catccaagaa cgaatttagc
    10921 gtgtttatgc caaacgctcg actatctgat gcatcttttc ctagttctga accgatatca
    10981 tcaatgatga ctaaatccgc tgttttgata tctgcaatca acgatccctc aatcgttttt
    11041 ctgagttcag gattgttgta cgaaaatttg atttgatcca gcatttcctg taacccgata
    11101 aataagattt ttttatcgta gtttgatcgc ttcaagactt cccaagccgc tgccatcgct
    11161 aggtgacttt tacctgttcc ttgttttcca gttaaaacaa ggtgacttgg atttcctaac
    11221 aagactgaat taacaaagcg tttggttact tcaaccgctt gtcttgtttc ttgatcgaca
    11281 atttgataat tctgcaatgt gcaatcaaac aacgcttgat taggcacaac agaaccacct
    11341 ttgaaaaaat tgattgctct tgctttcagg ctctcgttgt aaattcgttc tgtttgtaag
    11401 tcttcctgaa ctcgcaatgc tttataccca cattgcatac aggttggctt acaacgttct
    11461 gagccatcag ggttcttcgt acgccatcca tacaaaggct gtccgcattc aggacattcg
    11521 ccacgttgga caagcacttt ctgtatcagc ttttccatga tttccccaac agtttccatg
    11581 cctacgcctc ctctcaaata ggcaactcat ctgtgctaaa tttctcgtat tcaagcgttt
    11641 tagtttgttt tgttttataa tcacgatttg cttgcgctgc cattgtgtcg tatttttctc
    11701 gcaatttttt agcagataaa atattcgatg cccaaaacac attgtgctga ctccattcaa
    11761 tcattccccg tacctggttt tcggtccgct tgtcgatctc gatcattttc cgaatgtcat
    11821 ctgcccaact ttgcagattc ggctttttga tttcctgatt ctgacaaatc tgtttgaata
    11881 actcctccgc gagaatgtag taaactgagt cggtgtcgta aacacgcttt ttgcgtggtt
    11941 gcgacgatga tgttttatta ttcttttcat tcttataatt cttttcattc ttgtttgtgt
    12001 gcacttgttg ttcacttgtt gtgcgtttga tgttcatttg ttgttcactg ctttggtaca
    12061 aagaccagtt ttttattgat ataacgctgt atttcgtagt tgatttgatg ttcaacattc
    12121 cgtttttttc aaattgtttt agccatctcc atacagaacc gctgttcact tgatgttcac
    12181 gtttcacgcc ttcgttcatc tcagatgtga tagcatcgcg ccctgtgacg aattctccgc
    12241 tgttcacttg tatctctttt ccattaaaaa gtatcttgcg attctcatga ctcgctttca
    12301 tcaaacacaa gttccataat ttatacatat aaggattggt ccaaacgaat gaattcatca
    12361 cttttcgatg taatttgaca tatcctgtgt tcattcgtat ttctcctcta gaaaagcaag
    12421 ggagaaagct ccctcattat ttgtttaacg gcggattaga tgcatcaaat aatccagttt
    12481 gtacatcttc attttcttca gatataacct ctgcttcttt tctttcagga atatcttctt
    12541 caacttctgt ttcagcaata atgctgccgt cttcttgaac cctttggact ctctcatccg
    12601 atgtggtggc ttcttgcatt tcgatggaca agattcccca tttagaaaga agatttctca
    12661 aaacagtttt tcgtgccatt gcattgtaat cagatgccca cacaccactt aactttgtct
    12721 tatcgcgatc tttattgtta gcaatccgat gagcttcgat ttcttgtttg gtccaataga
    12781 cagttttttt gaatccattc agtaactcga aatagccaac atatccaatg acttcatcag
    12841 acgttctacc atttggatca aactcaaact cttctgtcag tcggttccag ctttttagtt
    12901 ctccttcgta aacttcgatc acatttaatg ctttgtattt acctgatcgt tgggctaatt
    12961 ggatatatcc tttatagcca agcatgaatt gagctttctt ttcccatttt cctgtttgct
    13021 tgtttttact attgaatgga actaaatatg cataacctaa attcttatct agcccaagat
    13081 ttaatgttgc agcagttaac gcaccactca tgatagacat cggttcacta tctgcaagat
    13141 aactgtcatt agatacaaga gtcataacat tcgacataaa agcattagca ttgtcatgaa
    13201 gcacctcttc gaacttcttt ctcattgttg gtgtattcat caaagcttta agtcctaatt
    13261 gacctggtgc aacttgtttc tgtggctttg ctgccaattg attttttaac gattcatttg
    13321 ttgccatatt atttgatctc cctttcggtt agccttcttg attcagtaac gttataaatc
    13381 tcttcatcat ttgcgacatc tggatatttc tctgctagtt tcttcgagtt catacgtctc
    13441 gtacggacaa atttccaact gatgatgttt ttttgagtga taccgatact ggcttcacgt
    13501 ttacctagct cgctgataat ctcgttgtct acttgacgga tagctgattc aatttctttc
    13561 ttagtccgtt tgagttcgtt tttctgttca actagttcat cgaaatgaat tggtaacgtc
    13621 gtttgaacgt cttcaacatc tgcatacttc tctttcaaga aatcagctgt cgcttgacta
    13681 ccatcaataa ttggctcgat acctccaaga acgtttgttt cccaaaactc tactaattgt
    13741 tcagtgattg tatcgatcag ctcttgatct cgttcaatcc gcttccagat aaatttttga
    13801 ccaccgatta agacagcgat gtaacaatag tctttgttta gaacgttcat gtaatgttga
    13861 acttgacaga gatagctaag cggtacctct tcgccttccc attctttacc gagaaattga
    13921 ttggctgttt tgcattccag aatggcattt tctcccacta cttcccgatc aatatttgct
    13981 cttaaaaatg gatgtaatgg atgttcaaat acttggtttc ttctgcgtac ttttttgcct
    14041 gtacgttcct gaaactcttt agcaaccact tcttctaaaa cattacccca ataagcgggc
    14101 tcacttcctg attcttcaag tactacttgt cctgtttttt ctagccatag ttgataagga
    14161 gatttccatt tattcaatcc taaaattgtt ccaacatccg aacctccgat gcctttctta
    14221 cggtcttcaa gccattcttg atggctcatt tctaaggtag atttactcat cttcatcctc
    14281 ctcatcaatt ggtgcttcat atggtggttt agcataatca ggatcagtta aatagttgtc
    14341 aagatttgct aactcgttca tgtttaacct cttttctgct tgctaacacg caaagcacaa
    14401 gcatggtaat tgcgaataac aagccaaaga ttacataact tttcgctaca atcactaacg
    14461 tgaaaaataa aataatgagt agatcaattg ttttttcatt catcattgaa attccccctt
    14521 gagaatacga gtcatcacat cagctaaatc ttccttgtta ttaataacaa aagtatgatt
    14581 tgaaggttga ttttcttctt catttttcgc ttgttcgtaa gcatccacag caattctacg
    14641 cataatatgt gctgacaatg ggagattttc atctaaatgt tcttcttgca tagcaagaca
    14701 aagtaaaatt tctgttgtct caccataaac gagactttta agttcccctt ctttttgggc
    14761 tgtacaaata atagaaacac cttgcttttg gcattcacgt ttcaattctt cgataagatt
    14821 ttcaattttc ttattcatgt gatataattc tcctagtttt ataaatttgt atgtgactct
    14881 ttgctggctg gcagagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
    14941 gcttgcttaa aataataggg cggctatttc cccaccgatt atcagcaatc actttaccga
    15001 tttttagcgc ttcttctcgt gccatagttg ctcctttctt ttgaatcaag cagattgatt
    15061 aaaaccatca atgctgcgaa caagcttccc ccgataatac tttggtgcgc tacaatcact
    15121 aataacccca aaatgaatcc tataaaaagt gtgtctgtct tcttcataat ctaatctccc
    15181 tattttttat ttctagcatt ctcaaatcct caagttcaga agcaattagt tcagcttgcc
    15241 tatctgatag ctcatcggct tttctaagcg ctgcacggtc atcttgtaat tgtttcctgc
    15301 gttgtttaat caaaccgaga atttgatgtt cttgttgcaa ggtatatgcc atatcacttc
    15361 tcctttcgtt tattgaattt ctccatttct atttgataaa atatttctat agaaatggag
    15421 gtgaaaacta tgggaaaaaa tcaacatgtt gtacctaatt ccaatggtgg ttggaatgtc
    15481 aaaggtgctg gaaattctaa agcgactgca catactaata caaaatcaga agcagtaaaa
    15541 atcgctagag agatttctaa aaatcaagga tctgagctat ttattcatgg cagagatgga
    15601 aaaattcaaa gccgtgatag tcacggaaac gatccacacc caccaaaagg ttaatcatat
    15661 ttaggagtta atcgaatacg ataaccgtca gcaggagttg catcggtcaa tgtaacttct
    15721 gctacttttt tcccttcttc tgtttcaata attaatcttg tgtaattgtt ctcattcaaa
    15781 atccctattt ttgattcttc cttcatgtca tccctccttc gtctctcgcc gtcaccacct
    15841 ccaagaagag gagttgccaa aatgaaaaaa cgtggacttt ggcaaacttg aaaggagagc
    15901 agcaactcct cttttcagaa gtggtgaatt tagatttttt agctattaac aaacagctct
    15961 aactctaaat cgatttttag aacgccatgt tctaaactgt tcatataaat cgacatcaat
    16021 tgttatttct ttggaagtaa ctgcgttata cccctcacta aaacgtgatt ttttaaattc
    16081 cttcattctt ctttgataag tactttcaga atacttatat ttctctttaa actccgcttt
    16141 gctcaaacgt tgtaccattt ttacgcctcc tgttctgatg gctgatcatt agaatctact
    16201 tctaacttga tatccaaaag ttcttcgatt tgttttaaac gttccttagg ttttcttttt
    16261 tcgatgaaga tatctcgtaa ataagtaatt gatataccca ttcgtcgagc taactctctt
    16321 tgactccaac ctttctttac taaagcaatt aatacttgct gttttaaagt catgcttcat
    16381 acccccttat cgaatatttt ctaatatttt tgtctaataa gtttgacaaa aactgcaaat
    16441 atgctattat gtaagcatac aaaatacagc gcacaaaaag cgagcttact gatgtctggg
    16501 gagactatta gtttattgct tttagtcttt gtatgccgta aatatcttgc ttacaaaact
    16561 agtataactg caaattctct atttgtaaat agataatttg cagaaaaata ctttttttgt
    16621 cggtattttg aaaggaatgc atatcctatg ctattagacc gtataaaaat cttattaaaa
    16681 gaacgttcaa tgacgttggc agaacttgaa agaaagttaa atttcggaaa tggaagcatg
    16741 tctagatgga ataaaagtac tccatctgga gataaaatac aaaaagttgc agattacttt
    16801 aatgtatcaa ctgattatct tttaggacgt actgataaac gccattatta tgatttaaca
    16861 gaaaaagata aacaagatat tgctttacaa gctgaaaaat tacttgaagg cttgaattca
    16921 gatgctgaaa caaattatta tggtgaacct ttagacgatg aagataagga aaaattatac
    16981 gatgccattc aacttgcact tgccttaacc aaagttaaag caaagaaaaa attcactcct
    17041 aaaaaatatc gtcaataggt gggctttggt atgcatttaa acaaatactg gatattagaa
    17101 gaaatagaaa aattatttgt agagtttgga tctgatgcga ttaatccata taaaataatc
    17161 aaatattctg atatagattt aattgaaact gatttaggga ataaaacatt aggacaaacg
    17221 atacacaata aaagatgtta tgtgatatta attaatcaaa acctatcacc ttctatgaaa
    17281 agatttgtat tatggcatga aatagcccac gttagactcc ataaaggtat ttgcacagca
    17341 gcttttagag caaacaacct tagttgtatg atttatggta tagaagctga agctaatatg
    17401 tttgcaattg aaatgttaac tagaacaata gatgaacaag atttatctga tatgagtcag
    17461 ttccaaatta ttgaatattt agggcttcct cataatcttg aacatttagt attacaacat
    17521 taaaaataaa aaagcccatg ctttcacatg gactctttcc tcatttctga gtaaactcta
    17581 ca
//
LOCUS       DAZUMI010000057        17314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000057 DAZUMI010000000
VERSION     DAZUMI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.057"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG81C_001651"
     CDS             complement(51..1598)
                     /locus_tag="ACG81C_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5064814.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG81C_001652"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG81C_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5064815.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACG81C_001653"
     CDS             complement(2043..2237)
                     /locus_tag="ACG81C_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064816.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            2320..2928
                     /locus_tag="ACG81C_001654"
                     /pseudo
     CDS             2320..2928
                     /locus_tag="ACG81C_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2948..3976
                     /locus_tag="ACG81C_001655"
     CDS             2948..3976
                     /locus_tag="ACG81C_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5064817.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            3973..4731
                     /locus_tag="ACG81C_001656"
     CDS             3973..4731
                     /locus_tag="ACG81C_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5064818.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(4817..5557)
                     /locus_tag="ACG81C_001657"
     CDS             complement(4817..5557)
                     /locus_tag="ACG81C_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5064819.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5618..6871
                     /locus_tag="ACG81C_001658"
     CDS             5618..6871
                     /locus_tag="ACG81C_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5064820.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            7166..7420
                     /locus_tag="ACG81C_001659"
                     /pseudo
     CDS             7166..7420
                     /locus_tag="ACG81C_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            7542..7868
                     /locus_tag="ACG81C_001660"
     CDS             7542..7868
                     /locus_tag="ACG81C_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5064821.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            7936..8649
                     /locus_tag="ACG81C_001661"
     CDS             7936..8649
                     /locus_tag="ACG81C_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5064822.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            8959..9811
                     /gene="licT"
                     /locus_tag="ACG81C_001662"
                     /pseudo
     CDS             8959..9811
                     /gene="licT"
                     /locus_tag="ACG81C_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            9932..11797
                     /locus_tag="ACG81C_001663"
     CDS             9932..11797
                     /locus_tag="ACG81C_001663"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5064823.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            11811..13254
                     /locus_tag="ACG81C_001664"
                     /pseudo
     CDS             11811..13254
                     /locus_tag="ACG81C_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            13362..14063
                     /locus_tag="ACG81C_001665"
     CDS             13362..14063
                     /locus_tag="ACG81C_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5064824.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            14189..14701
                     /locus_tag="ACG81C_001666"
                     /pseudo
     CDS             14189..14701
                     /locus_tag="ACG81C_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(15099..15308)
                     /locus_tag="ACG81C_001667"
     CDS             complement(15099..15308)
                     /locus_tag="ACG81C_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS5064825.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            15549..16124
                     /locus_tag="ACG81C_001668"
     CDS             15549..16124
                     /locus_tag="ACG81C_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5064826.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            16124..16564
                     /locus_tag="ACG81C_001669"
     CDS             16124..16564
                     /locus_tag="ACG81C_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5064827.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            16580..17281
                     /locus_tag="ACG81C_001670"
     CDS             16580..17281
                     /locus_tag="ACG81C_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5064828.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5715 a         2968 c         3577 g         5054 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacc cagaaaatca actttttgca
     2341 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctgctgc taagggtgtg
     2401 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     2461 gtttatcatg atgccccgcc ttttgcacct gctacgaaga ttgtagtaga aggtggtctc
     2521 tcatttcttg ccccttccaa actcgattgt ggtcagatca aagcaactgg gttgacgcaa
     2581 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaaca
     2641 aaaagtccat ggatcttaac gataaaatta tctcagcctt tcacaaccga tgcaggattt
     2701 tctatgcagg atgtcctata tttcaagaaa gctgaggagt atcaattagt gaatgaagaa
     2761 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aaacagatta ttcagaatat
     2821 ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     2881 tactcagcag agatgacatg gacattagca gatgcgccga cagaataaag atcaaggaga
     2941 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttctgat
     3001 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     3061 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     3121 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     3181 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     3241 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     3301 aacagtggga gaaataactg tccctcctca aagtgcggtc actgagacac tttcactgaa
     3361 agctccagaa aaagagctgg ctggtgtggt cgcaggagga atcacgtttc aagaaaaaaa
     3421 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     3481 aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat ttgaaattaa aaaaaactta
     3541 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     3601 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     3661 tctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     3721 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     3781 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     3841 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     3901 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     3961 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     4021 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     4081 tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     4141 ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa atcagggaac atccggatta
     4201 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     4261 acagtatatg ctgcattaca aaacggaacg gacagtacag ggacagcaac acaagtacag
     4321 aatttcgtac aagtaacgga taaaagcgga atttatgccg gttggaaact atcagtcaaa
     4381 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     4441 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaacgcc atctactatt
     4501 tctggtactg gcgctgaagg aattgagatc ccagcagatc aggcagtaga tctagtaact
     4561 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     4621 gggagacaat cagtggaatt atttgttcct gtatccaatt atgccgtaga caactacgtt
     4681 tcaacattca catggtcgtt aacagatgct cctacagaag ggaaagcgta aaatcaaatc
     4741 aatgacttca gggagtggag ccgattcaag gccaactccc tgaaatctgc tatgcttacg
     4801 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     4861 tcttttcaaa gatctttcga ccaacgagta aggagtcacc attgaaaaat tctaacacat
     4921 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaatagta
     4981 aaaagtaagc atacttttcc aactctgtct tcaattgttt taaagaagta ttcactaatt
     5041 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     5101 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     5161 cgacatttac gatttgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     5221 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     5281 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     5341 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     5401 aaacatcgtg attatcaata gtacccaact ggatcaactg attgatttga gagatagaag
     5461 tgagctgcac gatattctcg atatgatgat tgaccaattc tttgatgatc cattcgctgt
     5521 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     5581 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     5641 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     5701 agtaaaaaac agctttggtt ttatagcttt gatggtggtg acacttttat ttcctatccc
     5761 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     5821 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     5881 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     5941 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     6001 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     6061 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     6121 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     6181 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     6241 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     6301 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     6361 aatcttagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     6421 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     6481 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     6541 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     6601 tcagagtccg attacgatca atctgctgga agaaaaagag ggattacgtt ttagtatagc
     6661 gaacaaggca aatccgacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     6721 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     6781 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     6841 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag cttgaggaaa
     6901 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     6961 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     7021 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     7081 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     7141 tcgcttattt taggcgtgga gaagcaagaa aatccctacg atcacacaaa aaatgctgac
     7201 ccagcagtta agagagttag aacaagatgg cattgtccat cgaaaagtct ataaccaagt
     7261 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     7321 agcgatgtct gtttggggag aagaacgtat cgaaaaacaa aaagcctcag gggaagaagt
     7381 cacattactg catacacatg gtggctacct aaattactga tttctatgac atttatttta
     7441 aataaaagaa aacaggagtt tttttaaaag catcatctat tttttatgta aaaaaatggt
     7501 atgattaatc aagtaagaaa tgattggaag aaggcaagca aatgaatagc atggaaaaag
     7561 ttaaccagtt tagagatgaa cgtaattggc gacaatttca taacgaaaaa gatttggcta
     7621 tttctatttc tttggaagca tctgaattgt tagaactttt tcaatggaaa acgccggaag
     7681 aagtaaaaga aacacaatta gaacgaatca aagaagaact ggcggatgta ttgatctatt
     7741 catacatgat ggcagacaac cttcagctgg atctagatga gatcattgaa gaaaaattga
     7801 tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg tgtacgaaaa aaatacacag
     7861 agttttagaa atgtgtcatt cggctgttgg aaagagcaaa agaaaaacag taaaatgagt
     7921 ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa gatggctagt caatatcctc
     7981 ttgttcctct tgttattagt aggactagct ttgattttca atgaacagat caaggattat
     8041 cttgtcagag agacaggaga taaatatgct atagcgaatg taacaaaaga agatctgaaa
     8101 aagaataacg ataaagatgt cagttttgac tttgatgcgg tagaaccaat gacgacagaa
     8161 ggggtcatga ggtcgcagat gagaggaaca gatctaccag tgattgctag tatcgcagtc
     8221 ccttcagttt caatcaattt accgattttc aaaggcttgg ataatacgtc actactttac
     8281 ggagcaggaa cattatctcc agatcaagaa atgggaaaag gaaattatgc cttagcaagc
     8341 catcgtgcaa cgaaccctga attgttgttt acaccgctgg aaaatttaga gatgggtgcc
     8401 aagatctatt tgacagattt agaaaatgtg tatacataca aaacattttt taaagaaaaa
     8461 gttgctccta cagataccca attactgaat gaagtcgagg aaaaagaaat tgtcacacta
     8521 attacttgtg gcgacatgga cgccgtcacg cgactagttg tacaaggaga actggaaagt
     8581 gttacaccga taaaagatgc gacagatgat atgagatctg ctttcaatct agagacaaaa
     8641 acattttagg atactgggac ataagtcgtt acagattaaa aacagccaaa ggatatgaat
     8701 cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc aaagaggtgc atctctgatt
     8761 tggttgtttt ctagatagac ttgtgttcca gtttttttat tttttatatg aaaaggcttg
     8821 cataaataaa aattcgatgg tatgatgatt ttgaataaga aatggattgt tactgtacgg
     8881 caggcaaaac cagaattcag ctaagaaaca gtaggcgaat tctaggtttt tttatttttc
     8941 ttgagagggc gaagcaaaat gaaaattgat aaaattttga ataacaatgt cgtcatttcg
     9001 aaaaatggat tcggggaaga agtcgtttgt atgggaaaag gactagcttt tcaaaaaaaa
     9061 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttattttaa gagattcttt
     9121 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
     9181 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
     9241 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
     9301 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
     9361 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
     9421 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
     9481 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
     9541 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
     9601 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
     9661 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
     9721 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
     9781 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
     9841 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
     9901 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
     9961 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    10021 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    10081 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    10141 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    10201 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    10261 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    10321 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgcag cagatggtgt
    10381 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    10441 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    10501 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    10561 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    10621 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    10681 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    10741 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    10801 aagtttatgg caagtattcg tgatgttcgg catgcattgg ggattcgtac cgatcatgtt
    10861 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    10921 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    10981 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    11041 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    11101 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    11161 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    11221 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    11281 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggagaac cgattacttc
    11341 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    11401 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    11461 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    11521 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    11581 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    11641 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    11701 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    11761 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    11821 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    11881 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    11941 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    12001 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    12061 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    12121 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    12181 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    12241 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    12301 cagacagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    12361 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    12421 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    12481 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    12541 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    12601 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    12661 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    12721 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    12781 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    12841 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    12901 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    12961 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    13021 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    13081 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    13141 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    13201 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    13261 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    13321 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    13381 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    13441 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    13501 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    13561 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    13621 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    13681 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    13741 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    13801 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    13861 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    13921 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    13981 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    14041 accatattga ttatgaaaca taaataaaaa aagaggttgt gaaaaagctg ccctgcatca
    14101 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    14161 accgcagata cacggctgtt tctggattac catttattct ggataagggg gtgtggcacg
    14221 attttcagaa ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc
    14281 attcggcttg acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tactcaatcg
    14341 tcaagaaatc gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg
    14401 atgtttccct atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg
    14461 gacagtaggt ggcggattaa gcatgttttg gggcgcagat agccgtattc cggcatcggg
    14521 aatgtttgca gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg
    14581 ttcttttgtc acaggaataa ccattttatt attgaaaaaa ccagtagatc cgaatgcaga
    14641 aatcatccaa gaagaaaaag aggaagaaga catatcttgg gatgatttga caattagtta
    14701 ggagaggcag aatggattac aagaaaaaag tacaatagat gtaccttgag acaggaatgg
    14761 tatgaaagaa tcagagttag aaagaatcga atatacagat tttggtttag gagcgatcga
    14821 aactgtccag agcatcggca ccctccttga aatgacgatg aaggaaaaat gagaaccttt
    14881 cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct
    14941 gtactattcc aaaaaggaaa gaaagttatt atacagtaaa aaaagaaatt attcttcatg
    15001 ctagagatca gtacacgttc gagcccaata tcaaacacta gttccaggca ggcgcaacag
    15061 gtgcggttat cactgaattt tcttccagca gtgacgattc aagtgatatt tttacaaatc
    15121 cagctattta tcgttaacaa aaagattttt aggagaagct gatgaacaaa aatgatcaaa
    15181 ttttgatcgt ttagttcgtc ggctttttta tttagagaaa catcatttat cagtgaaaca
    15241 taacacgaga acaaaatgat tttttcgtta ataaaagaaa caagtatttg ttgtttaaaa
    15301 taatacacgt tattggcata taggacgaaa tcccagtatc ttctataagt aagtcatttc
    15361 atatcgatga aaacacgtta tttatttttg ttttatctat aaaatggctg aaaaatgatt
    15421 tttttctaaa tactatatat agtggcgtct atcctatata ccactaaaaa actacaaaat
    15481 ctagtctttt attttaaaaa aacacgtgta gaatgaagat agacttttga gttagggagt
    15541 gtttttttat gaaacttact gttttttgcg gttcccgttt tggtaataaa gaaagttaca
    15601 aatttattgc gcaaacgtta ggaaaataca tggcacaaga agaaatcgaa ctggtttacg
    15661 gtggttcata ctccgggatt atgggagtga ttagtaaaac agtactggaa aataacggaa
    15721 aagtcacagg tatctatccg aatgggcttt ttgaaactga attgcctgga aaagatgtta
    15781 ctacatttat tcctacagaa acgatcgatg aaagaaaggt attattattt gaaaagggag
    15841 atgctgtcct cgttttccct ggaggtctag gtacattgga ggaattctca caacttcttt
    15901 cttggatagc aatcggtctg acgcccgaca agccaatcgg aatattgaat attggcggtt
    15961 attacagcgg cttgcaacaa ttattagaaa cttttgcaaa agaggatttt atggataaaa
    16021 aatggttgga tcaagtaatc ttttccaaca atccattgga actggtcaat gaattacgag
    16081 cagtatctat taaaaatcag ttattattga aggaggcaaa ataatgagtc aaatatattt
    16141 agcaggacca tttttttcag aggagcaaat cgatcgagta agtcgtatcg aaaaagcgtt
    16201 ggaggaaaac aaaacagtca caagtttcta ctctccacgt catcaccaag aaagcaatta
    16261 tgaactattc agcgctggat gggcgcagga agtatacgaa aaagatatgg aagaactgac
    16321 aaatgcagaa tttgttgttg ctatccttga ctttgagcat caaacaatcg atccaggaac
    16381 ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg atgatcattg tacaagaaga
    16441 gacggtacct acgaacttga tgattacaca aagtctccat acgtatttaa aatcagacca
    16501 agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa acttacgtag gcgagtactt
    16561 atagaagagg aagggttgta tgtcagtgat tttgatttca ggaacgattg gtgcaggtaa
    16621 gagcagtttg acggatatgc ttgcaaaaga gattgattct aaaccatttt atgaaaatgt
    16681 cgaagataac gaagtattgc cgctctttta ttcaaatcct gagcagtacg cttttttatt
    16741 gcaaatcttc tttttgaaca agcgattttt agcaatgaaa aatgctttag tgaacgatga
    16801 caatgtgcta gaccgatcca tctatgaaga tagcctgttg ttccatctaa acgctgattt
    16861 aggtcgggta acggatatcg aagtacagca gtacgatagc ttattagata cgatgctaaa
    16921 cgagttagat gatgttgctc caaaaaaacg gcctgatttg atggtccata tcaaagtttc
    16981 tttggacacg atgctagaac gtatcaaaaa acgtgggcgt gactatgaac agctagaaag
    17041 cgatgagacg ttatatacct attatgaaac gttgaacacc cgctataatc aatggtatga
    17101 agattttgat gtatgtccta aaatacaagt ggatggggat aaattcgatt ttgtagaaaa
    17161 tccagaagat gcaaaaaagg tgatccgtca gatccaagaa aaattagctg aattggaagg
    17221 aaagcctgct ggaaaatatt ttacggataa ccagcatgca aaaggtcaag aattaggcta
    17281 aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAZUMI010000058        17307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000058 DAZUMI010000000
VERSION     DAZUMI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.058"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..551)
                     /locus_tag="ACG81C_001671"
     CDS             complement(6..551)
                     /locus_tag="ACG81C_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5064829.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(563..1255)
                     /locus_tag="ACG81C_001672"
     CDS             complement(563..1255)
                     /locus_tag="ACG81C_001672"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5064830.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1274..1558)
                     /gene="macP"
                     /locus_tag="ACG81C_001673"
     CDS             complement(1274..1558)
                     /gene="macP"
                     /locus_tag="ACG81C_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5064831.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1571..2134)
                     /locus_tag="ACG81C_001674"
     CDS             complement(1571..2134)
                     /locus_tag="ACG81C_001674"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5064832.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2362..3024
                     /locus_tag="ACG81C_001675"
     CDS             2362..3024
                     /locus_tag="ACG81C_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5064833.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3148..4344
                     /locus_tag="ACG81C_001676"
     CDS             3148..4344
                     /locus_tag="ACG81C_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5064834.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4445..5821)
                     /locus_tag="ACG81C_001677"
     CDS             complement(4445..5821)
                     /locus_tag="ACG81C_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5064835.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5858..6724)
                     /locus_tag="ACG81C_001678"
     CDS             complement(5858..6724)
                     /locus_tag="ACG81C_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5064836.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6813..7838)
                     /locus_tag="ACG81C_001679"
     CDS             complement(6813..7838)
                     /locus_tag="ACG81C_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5064837.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7856..8530)
                     /locus_tag="ACG81C_001680"
     CDS             complement(7856..8530)
                     /locus_tag="ACG81C_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5064838.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8714..9607)
                     /locus_tag="ACG81C_001681"
     CDS             complement(8714..9607)
                     /locus_tag="ACG81C_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5064839.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9681..11378)
                     /locus_tag="ACG81C_001682"
     CDS             complement(9681..11378)
                     /locus_tag="ACG81C_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5064840.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11371..12684)
                     /locus_tag="ACG81C_001683"
     CDS             complement(11371..12684)
                     /locus_tag="ACG81C_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5064841.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12684..16625)
                     /locus_tag="ACG81C_001684"
     CDS             complement(12684..16625)
                     /locus_tag="ACG81C_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5064842.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16734..17192)
                     /locus_tag="ACG81C_001685"
     CDS             complement(16734..17192)
                     /locus_tag="ACG81C_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5064843.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4853 a         3892 c         3009 g         5553 t
ORIGIN      
        1 tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt
       61 ccaacgggat cgaaacttgc cacggaatct ttgaaaatat ggagtttgta tcctagatta
      121 tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata
      181 tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa
      241 tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt
      301 aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg
      361 ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc
      421 ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct
      481 gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc
      541 aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg
      601 ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg
      661 ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc
      721 caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt
      781 tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt
      841 tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat
      901 aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt gcatctgcat
      961 caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc
     1021 cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg
     1081 tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac
     1141 ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt
     1201 tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt
     1261 taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa
     1321 caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga
     1381 tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct
     1441 ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg
     1501 cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa
     1561 gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct
     1621 ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat
     1681 acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt
     1741 gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa
     1801 gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca
     1861 tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt
     1921 tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa
     1981 tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct ccatctggca
     2041 aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga
     2101 ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca
     2161 taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga
     2221 taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac tctttctagt
     2281 agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat
     2341 aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc
     2401 ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata
     2461 ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa
     2521 gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg
     2581 aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg
     2641 caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc
     2701 aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca
     2761 agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag
     2821 atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag
     2881 atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat
     2941 ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa
     3001 tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag
     3061 atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag
     3121 aacagattca acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt
     3181 acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat
     3241 acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct
     3301 cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa
     3361 atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta
     3421 agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga
     3481 gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc
     3541 ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg
     3601 acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa
     3661 gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa
     3721 gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt
     3781 caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat
     3841 gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat
     3901 cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac
     3961 acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg
     4021 aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa
     4081 cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc
     4141 tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa
     4201 accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga
     4261 aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa
     4321 aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac
     4381 aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg
     4441 gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc
     4501 caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata
     4561 caatacaagt caaaatggag acgattggca atagcggaac gaatggcact ttgaattctc
     4621 cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca
     4681 aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg
     4741 caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt
     4801 gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac
     4861 gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag
     4921 cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat
     4981 tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa
     5041 ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt
     5101 tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag
     5161 ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt
     5221 taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca
     5281 tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata
     5341 tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag
     5401 ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc
     5461 atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca
     5521 gccaggcagc aaattcacca aatacaacat acagataggc atatggccca ccaatcactg
     5581 gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta
     5641 agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca
     5701 caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta
     5761 gttctttttt catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca
     5821 ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta
     5881 aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt
     5941 tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt
     6001 gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt
     6061 ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa
     6121 ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa
     6181 ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg
     6241 tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta
     6301 tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag
     6361 caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg
     6421 caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca
     6481 tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa
     6541 atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag
     6601 aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt
     6661 taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt
     6721 ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa
     6781 cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag
     6841 attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc cgtcaaatgg
     6901 atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt
     6961 ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc
     7021 gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct
     7081 atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg
     7141 ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc
     7201 tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg
     7261 attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca
     7321 taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta
     7381 tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt
     7441 ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg
     7501 attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc
     7561 agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt
     7621 atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa
     7681 atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg
     7741 tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc
     7801 aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg
     7861 gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga
     7921 tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt
     7981 catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca
     8041 aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca
     8101 tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt
     8161 ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt
     8221 ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat
     8281 tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac
     8341 accaataata gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt
     8401 ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc
     8461 atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga
     8521 tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct
     8581 tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga
     8641 aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt
     8701 aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat
     8761 aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc
     8821 gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa
     8881 ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa
     8941 gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa
     9001 caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt
     9061 catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg
     9121 cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct tgcaggactg
     9181 tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga
     9241 ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt
     9301 ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg
     9361 atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt
     9421 cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat
     9481 tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca
     9541 ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct
     9601 ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg
     9661 ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg
     9721 tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca
     9781 gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact
     9841 cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct
     9901 gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt
     9961 ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct
    10021 tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac
    10081 tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca
    10141 tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg
    10201 actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca
    10261 ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta
    10321 tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg
    10381 gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact
    10441 atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca
    10501 taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt
    10561 ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc
    10621 gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata
    10681 atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt
    10741 tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt agaaccatcc
    10801 cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg
    10861 attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa
    10921 gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt
    10981 gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt
    11041 tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg tgttaaagga
    11101 ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct
    11161 ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga
    11221 acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact
    11281 tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac
    11341 agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt
    11401 tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca acgttcttgc
    11461 ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc ttggaccact
    11521 cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac gtggtgttgc
    11581 ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct cctgtccgtt
    11641 aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga tgatgacctc
    11701 gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt cttcaaaata
    11761 ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt tatccgaaat
    11821 gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga tccattgatt
    11881 tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta tcatctgata
    11941 tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc tttgacgtcc
    12001 atacttgaag aagtatggat ctcttagata accgacattt ttaggataga tatgaatctc
    12061 ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag caattgggat
    12121 agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt cttcgaaaat
    12181 caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg caatcccgtc
    12241 tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt ggacaagcct
    12301 ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt gctggacgaa
    12361 ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg tcatgtcgta
    12421 aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg ctgtttcccc
    12481 tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat cgttataatc
    12541 gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc ctgattcttc
    12601 tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa gtaattgaag
    12661 catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg tctgacaaac
    12721 aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat atagaataat
    12781 cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac actaagctgt
    12841 aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag atcaggcgtc
    12901 tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc ttcttttcct
    12961 ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc taaaataaag
    13021 taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg atagacccct
    13081 tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc caagcctccc
    13141 tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc tgcgccttca
    13201 agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg cgctagttca
    13261 tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg caagggagca
    13321 gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt cagtgcaaac
    13381 tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga gacctcatat
    13441 tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt ttctactcct
    13501 acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc tggttctaac
    13561 ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt cctgatatcg
    13621 aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg gatctcccga
    13681 taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc atctgttgta
    13741 tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt ccctatagca
    13801 ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa accttctggg
    13861 ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact atccttataa
    13921 acaaatgaat ctggctctcc gattggatga ataactttct caccatccac gattacatat
    13981 ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc tacttcgttc
    14041 acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc atctgtttca
    14101 taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag tttattgata
    14161 ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa ctcatcatac
    14221 tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc tccttcataa
    14281 taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt atattcatct
    14341 acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt gatttggaat
    14401 tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct gatccaaaat
    14461 atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc aaagatcgct
    14521 attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact ctctggattt
    14581 tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc cttcttgctg
    14641 acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa atacatccgt
    14701 ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat attgttcact
    14761 gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt gatcctgacc
    14821 atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc ccgtccgaca
    14881 tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc ttcatcccat
    14941 atttctggct cttggtactt catgccgttt tctcctggat ctagatggta aaactgaata
    15001 tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc ataaagtgtg
    15061 gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt aacaatgtca
    15121 ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa atcagcaata
    15181 tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac gtttccatcg
    15241 attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt tttcagccaa
    15301 tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg actgccattc
    15361 gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc tccttctttt
    15421 acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa gtcagatgca
    15481 taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc ctcaaaattt
    15541 ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca gttgctccaa
    15601 gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa ggtcggtgtc
    15661 ccatctccca caacgattag gattttttct ggatttctat tttctccgcc attgtcttta
    15721 tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc gattggtgta
    15781 cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt caatggaaag
    15841 atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta aaaattcacc
    15901 ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat ttcgtttact
    15961 gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa ttcactcata
    16021 ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt tttctcgccc
    16081 ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg tagatcagat
    16141 gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc gttataatcc
    16201 aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg agctggaacg
    16261 tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta atttcgccag
    16321 ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc atatggcgag
    16381 cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac ttttgtatag
    16441 tcattcgttg taaacttcgg gtagcgatgc tgttcgtttt caacttctcc gctaggatag
    16501 atatattcat aggggtcttg cgtagctgtt ggcccaagac gctccaaaat accgttgaat
    16561 aactggcgtt gaatgcgctg gagtgcttga ccgctgggta cgattggttc gtccagcttt
    16621 tccatctcat tttcttcgct acgttgaatt tcagcggaat cttctgtatc agtttcattt
    16681 tcttgtaatt cttggttatc tattctttca tcaaccacca cttgaagagg tagttaccct
    16741 tcgttcacat cggggatctt ttctgtttca aaaatcaatg tgccggtttc tgcttctttt
    16801 gaataaactt ttccgacata ccattcttga ccatcgatcg tctcgacttt taggtcttct
    16861 ccatcttcat cataggagga tagacctgtt ccacgaacgt tttttacggt accaatcccg
    16921 gcggctgccg catcgattgc taaacgtatt ctaccttcat tggcactttc ctttttttca
    16981 aaagccagat gccattcaag ggtttcttct ttttcaatat acttcccttc aatttttgca
    17041 tagtcctcat ctaagaatac ctcatcctcc tttgtttctg ttgactcagc agctacatcc
    17101 agggtcgtca tatgatagac tgactggaaa tatggtccaa atagcaaact gacagttact
    17161 aactgcaaaa gcttcaattt taaccctttc acatccttat actcctttca aaaatatttg
    17221 tcttctttca caacaatcaa caaaacaatg cccatctaat tataatttaa tttaatttta
    17281 attttaaatc aaatgatatc cttttta
//
LOCUS       DAZUMI010000059        17053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000059 DAZUMI010000000
VERSION     DAZUMI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.059"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG81C_001686"
     CDS             complement(33..1076)
                     /locus_tag="ACG81C_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5064844.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG81C_001687"
     CDS             complement(1237..1920)
                     /locus_tag="ACG81C_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFS5064845.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG81C_001688"
     CDS             2115..3044
                     /locus_tag="ACG81C_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5064846.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG81C_001689"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG81C_001689"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS5064847.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG81C_001690"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG81C_001690"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS5064848.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG81C_001691"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG81C_001691"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS5064849.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG81C_001692"
     CDS             5429..6232
                     /locus_tag="ACG81C_001692"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5064850.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG81C_001693"
     CDS             6316..7863
                     /locus_tag="ACG81C_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS5064851.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG81C_001694"
     CDS             complement(8160..8357)
                     /locus_tag="ACG81C_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064852.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG81C_001695"
     CDS             complement(8433..8633)
                     /locus_tag="ACG81C_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5064853.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG81C_001696"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG81C_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG81C_001697"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG81C_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5064854.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="ACG81C_001698"
     CDS             complement(11515..12297)
                     /locus_tag="ACG81C_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064855.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="ACG81C_001699"
     CDS             complement(12362..13198)
                     /locus_tag="ACG81C_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5064856.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="ACG81C_001700"
                     /pseudo
     CDS             complement(13216..14625)
                     /locus_tag="ACG81C_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(14639..16462)
                     /locus_tag="ACG81C_001701"
     CDS             complement(14639..16462)
                     /locus_tag="ACG81C_001701"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5064857.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         5052 a         3462 c         3191 g         5348 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggctat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
    17041 ctgatttatt tct
//
LOCUS       DAZUMI010000060        16921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000060 DAZUMI010000000
VERSION     DAZUMI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.060"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(244..2136)
                     /locus_tag="ACG81C_001702"
     CDS             complement(244..2136)
                     /locus_tag="ACG81C_001702"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064858.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2133..3860)
                     /locus_tag="ACG81C_001703"
     CDS             complement(2133..3860)
                     /locus_tag="ACG81C_001703"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064859.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3876..4526)
                     /locus_tag="ACG81C_001704"
     CDS             complement(3876..4526)
                     /locus_tag="ACG81C_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5064860.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4730..5026
                     /locus_tag="ACG81C_001705"
     CDS             4730..5026
                     /locus_tag="ACG81C_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5064861.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5198..5818
                     /locus_tag="ACG81C_001706"
     CDS             5198..5818
                     /locus_tag="ACG81C_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5064862.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5910..7076)
                     /locus_tag="ACG81C_001707"
     CDS             complement(5910..7076)
                     /locus_tag="ACG81C_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5064863.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7088..7441)
                     /locus_tag="ACG81C_001708"
     CDS             complement(7088..7441)
                     /locus_tag="ACG81C_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5064864.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7569..8591
                     /locus_tag="ACG81C_001709"
     CDS             7569..8591
                     /locus_tag="ACG81C_001709"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5064865.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8610..8771
                     /locus_tag="ACG81C_001710"
     CDS             8610..8771
                     /locus_tag="ACG81C_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064866.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9030..9464)
                     /locus_tag="ACG81C_001711"
     CDS             complement(9030..9464)
                     /locus_tag="ACG81C_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064867.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9574..11433)
                     /locus_tag="ACG81C_001712"
     CDS             complement(9574..11433)
                     /locus_tag="ACG81C_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5064868.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11434..11574
                     /locus_tag="ACG81C_001713"
     CDS             11434..11574
                     /locus_tag="ACG81C_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5064869.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11551..12711)
                     /locus_tag="ACG81C_001714"
     CDS             complement(11551..12711)
                     /locus_tag="ACG81C_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5064870.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13018..13929)
                     /locus_tag="ACG81C_001715"
                     /pseudo
     CDS             complement(13018..13929)
                     /locus_tag="ACG81C_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(14095..15288)
                     /locus_tag="ACG81C_001716"
     CDS             complement(14095..15288)
                     /locus_tag="ACG81C_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5064871.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(15372..15572)
                     /locus_tag="ACG81C_001717"
     CDS             complement(15372..15572)
                     /locus_tag="ACG81C_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS5064872.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(15996..16229)
                     /locus_tag="ACG81C_001718"
     CDS             complement(15996..16229)
                     /locus_tag="ACG81C_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5064873.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(16236..16667)
                     /locus_tag="ACG81C_001719"
     CDS             complement(16236..16667)
                     /locus_tag="ACG81C_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5064874.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4751 a         3696 c         3178 g         5296 t
ORIGIN      
        1 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag
       61 gttgtaaaat cagcggtttg acctgagtat tagagataca aaacggaaaa atagcttctc
      121 atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc gtttattcgg
      181 aaaaagggac tgtgacacga cttttgtcac agcccctaaa aaaaataata aatttgtact
      241 attctatgaa gcttcttctt cagaaaactg actgttataa agatctgcat agaatccgtc
      301 tttttccatc aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat tcatcacaat
      361 aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt
      421 ttccaatagg cggttcatcg ctttttgaat cagtatttct gttcgtgtat ctacactaga
      481 tgttgcttca tcaaggatca ggacatctgg atttgctaag aatgcccgcg caattgtgat
      541 tagctgacgc tgcccttgcg aaatattgct tgcatcctcg tttaatacag tgttatatcc
      601 ttctggcaat ttacgaacaa aatcgtctac gtgagcagct ttagcagcct caatgatttg
      661 ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca tagatacttc ctgtaaacag
      721 ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg
      781 ggtatctaca ccatcgtaac gaatactacc tccagaaaca tcgtagaacc gttcaagaag
      841 gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca atagcgacca tctcaccagg
      901 tttgacatcc aagttaaaat cagtcattag caagttatct ggtgtgtagc cgaattgaac
      961 attttcaaaa cgaactttgt atggtgtatc tttttcttct ggaaggccag atttttcatt
     1021 ggacatctct tcttcatcca acacttcaaa tacccgttcg gctgaagcaa ctgttgattg
     1081 gatagtgttc atcaaacttg caagttgagt gattggttga gagaactgtg ttgtatattg
     1141 caagaacgct tgaacatccc ctaatgtcat gttcccattt gcaactttta ctccgcctag
     1201 tacagcgacg aacacatagc ctagattttt aacgaaattc atcaacggca tgatgattgc
     1261 cgaaataaat tgagctttcc aaccagcggc atacagattc tgattttctt tttcaaatac
     1321 ttcctggtct ttttcagtat gattgaatgt tttaacaatg gtatgaccac catatgtttc
     1381 ttctacttgg ttattcaata aacctaaact tttttgctga tctgcgaaaa acacttgtga
     1441 tttcggcgct acgatcatca cgatgatcaa gcttaaaggg atcatcaata acgcgactaa
     1501 cgtcaattgc cagctgattg aaagcatcat cacgatgacc ccaatcaatg tgactgcact
     1561 agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca tatcattgat
     1621 tgctcgagac atgatatccc catttgtatg tgtatcataa taggaaatcg gtacacgatt
     1681 cattttggct tccaaatcat gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat
     1741 aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga ttactgtcgc
     1801 aatgatctgc gcgattttat caaagtctat cggaaatttt gttacttgtt ggccttgttg
     1861 catctgttga gcgccagcca ttacaccttt gaatatttct gtggttgctt tccccaagac
     1921 ctttggtgtt tggatttgga agatcacggc tgctattgct agtacaaata cagcgatgat
     1981 cgcaaccata cgtttagaca tatagccaaa caatcggcgt accgttcccc agaaattttt
     2041 aggcttttca ccttttacac cgagatttct gccaggtcca ccatgtcctg gaccgcctgc
     2101 tggtccatga gagggacgtg tgtttgtttt actcatgcta agtcctcctc tcttagttgt
     2161 gaatgaacga tttcttgata agtttcgttt gttgctaata gttcttcatg tgttcctttg
     2221 ccaaccagtt ttccttcatc tagcacaagg atcatatctg catccattac tgtacttact
     2281 cgttgagcaa ctaaaacggt gatactttct ttcatattcg ttttcaatgc ttggcgaaga
     2341 ttggcatccg ttttgaaatc caacgctgag aaggaatcat caaacacata gatatctgct
     2401 gttttgacta atgcccgtgc aattgccaga cgctgtcttt gtccaccaga gaagtttcct
     2461 cctccttgtt ccacatggct atctaagcca tcttccatct ctgaaacaaa atctttagct
     2521 tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg catccggctt accatactgc
     2581 atattgtctc ggatcgttcc agagaacaga actgcttttt gaggaacaaa gcccattaat
     2641 tctcgcaaat tatattgagt catgtcacta atgtccgttc catttatttg gatcgaacca
     2701 gattcgatgt catataatcg agggatcaaa ttgactaagg tcgttttacc tgaaccagtc
     2761 ccccctatga ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc ttccaatgca
     2821 agtttttctg ctccatgata tcgataattg acatgattga aggccaatgt tgcgtttttt
     2881 ccagcaaaag aaactgtttt aggattttca gggtcatgga taccgatttt ttcatccaac
     2941 acttcattaa tacggtcagc tgaagcttgt gcacgaggca ccatgataaa aatagcggaa
     3001 agcatcataa agctgatcaa gatctgcatc gcataagtca taaatgcaat cagattaccg
     3061 acttccaaag tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta
     3121 ccgctcatga tcaatgtcat cattggcatc atcaatgcga cgatcgtatt aactttgatt
     3181 gccgtatttg tataatcttt attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta
     3241 tcgaatgctc gaatcactct gacccctgtc aatccttctc gaaataccag gtttaaacgg
     3301 tcagttttct tttgcatact tttgaataat ggaacagcaa agtacatgat tccgccgatc
     3361 aaaataatca ttactgggat gacaactaag aagatttttg tcaactgatg atctttttga
     3421 taagcaagta tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat acgtaaaaac
     3481 atttgtgtaa ccatttggat ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc
     3541 gttccatatt tatcaaacgc atcttttgtc agactctctg attttttata gacatccgta
     3601 cgcaattgct tacccagttt ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca
     3661 gctaagatac tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca aatataatcg
     3721 acatctcctt gcgctacccc tttatcaata atattggagg taagtgtcgg caaatataaa
     3781 tctgccagta cttggatcac catgaaaact gcagctgcaa ttgctgaagt caaggagatc
     3841 cgttttacga tttttagcat ctagttccct tctttctatt tatatactcc gtttttcagc
     3901 caagaaagtt tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt
     3961 gaaaataagc gatacgaatg cgcaattgtt gtaaatacag tattcatcac taattggatc
     4021 agtaattcca attcatgatc attttttact ttcagcttcg tccgatcgat caattcaagc
     4081 aactcttttg catcttgttt gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc
     4141 gtatgaggag cgacttttct tgaggcacgg taatccatgt tcataaacaa gttacgataa
     4201 aaagaagcat tctcgccttc caaaatctca taaatcattt tagaaaaata ggattcgaat
     4261 cctttgaata gatcaccatc ggattctttc attagttgta acatatcttt agagctgtca
     4321 cgtctcattg tctcaaagta ataaaaataa agatcctctt tatcttcaaa atactgatag
     4381 aaactgcctc tagggatatc cgctaatttg acgatattag caatcgaagc ttcttttaag
     4441 ggtactctag aaaactcgat tctcgcagcc tcgattagtc ttttttgttt ttctttaggc
     4501 aaatggaaaa atgtctgtgt cggcatatgg atcctccttt ctttcaagat gacacattgt
     4561 catatgacgc tgtgtcatta taacacctcc tacctgtttt gcaagacatt tgcatagttt
     4621 ttttcattat catccgatac attaaaaaag tgataagagg aatccttcca tttgaattaa
     4681 tgagagttgc ccattaaaat agccacatac taaacaaagg atgagataaa tggcccaatc
     4741 aattagtgtc caagacttca ataaattgcc tctcgatgct gacagcatcg ttttagatac
     4801 aagaaatacc ttagattaca caaaaaacca cttaagccac tcgctttcta tccccgctgc
     4861 tatcttgcca agacaattaa aaaatctaga tccaaagaaa ctttattata ttctaagcta
     4921 tacaggcagg cgatcagaag cattagccga tcaacttact gctaatggtt ttcgtgctgt
     4981 ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat tgcttgttag
     5041 attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc tgtctttttt
     5101 tagttattta ttcttcaacc ttatcaatat gacttgatag ccttttcttt ttctcatagt
     5161 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5221 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5281 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5341 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5401 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5461 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5521 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5581 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5641 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5701 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5761 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5821 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtacc
     5881 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5941 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     6001 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     6061 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     6121 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6181 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6241 aagtaaggga tcaggttcaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6301 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6361 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6421 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6481 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6541 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6601 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6661 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6721 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6781 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6841 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6901 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6961 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     7021 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     7081 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     7141 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7201 cgtgagctac tgtctctaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7261 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7321 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7381 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7441 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7501 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7561 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7621 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7681 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7741 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7801 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7861 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7921 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7981 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     8041 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     8101 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     8161 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8221 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8281 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8341 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8401 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8461 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8521 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8581 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8641 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8701 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8761 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8821 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8881 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8941 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     9001 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     9061 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     9121 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9181 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9241 cgcaaagtac tgactttctt aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9301 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9361 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9421 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9481 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9541 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9601 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9661 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9721 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9781 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9841 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9901 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9961 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
    10021 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
    10081 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
    10141 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10201 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10261 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10321 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10381 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10441 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10501 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10561 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10621 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10681 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10741 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10801 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10861 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10921 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10981 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    11041 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    11101 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    11161 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11221 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11281 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11341 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11401 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11461 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11521 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11581 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11641 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11701 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11761 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11821 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11881 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11941 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    12001 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    12061 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    12121 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12181 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12241 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12301 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12361 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12421 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12481 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12541 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12601 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12661 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12721 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12781 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12841 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12901 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12961 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    13021 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    13081 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    13141 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13201 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13261 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13321 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13381 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13441 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13501 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13561 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13621 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13681 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13741 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13801 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13861 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13921 aaaattatat gggattttag attatctttt tagaagttct tgaatgtcgg tatagcccgt
    13981 gtttccgggc ttttccggca tttttgatat tgttagatac ttacaaatgt tcttattacc
    14041 tctcaccatt tccccctcaa aagtagtaaa tggagtagta aaaccggcct tgtgtcaggc
    14101 tgccagccgt tccatttctg cccttgcgga atcgaatgtg gcgtgtgcat aatagttcag
    14161 cgtcatggtg atattggaat gtcccataat gtactgcaag gctttggggt tcattcccgc
    14221 attggctaac cgggtgcaga atgtatgacg catagcatgg ggcgtcgtcg ttttcggcaa
    14281 cgcttcttca tggctcttgt tgtacttctt cacaagcctg ccaaacatcc cgccataatc
    14341 taccgccgtc atgggatagc cgttctgttt gagaaacagg aaattggcat acccgtcaat
    14401 aatgaacggc tttgcacctt tgcggttttt tatcactcgc tggaacgcct gatacacttc
    14461 ctcattcatg gggatttttc tgacgccgct ttgggtcttg ggttcgtcaa tatagtaacc
    14521 gacgccggta tttctcaaaa gctggtggct cacgatgatt acccggtttt caaaatccaa
    14581 atctttgtcc gtcagtccgc acagttccga aatgcgaagc cctgtcccca gcagaataac
    14641 gattgcgtca taatgctttt ggtaaacctt atcaccttga ataaaggaca gaaaactttc
    14701 ttcctgtgct ggtgtaagag gcacctttgg ttccgtatcg tcgtccagaa cgtcactaag
    14761 ctggaagtca aacgggtttt ttcggatgca atcgtcctgt atggcggtgt aaaaagccgc
    14821 tttcagggaa cgcttgtcgt tattgatggt cttataagaa aggccctttt ctttcatccg
    14881 aagcgcccat tcctttgcgt cggacagctt cacactgtca atcgggcagc tccccagccg
    14941 atcttgttcc agaatacgca tcagccgttc ccggcccagt ttggtgttgt gcttcacatt
    15001 gccccggtgc ctgatctgct tggcgtagag ctggcagacg gtcattttct tgccgatggt
    15061 gtctatcccg tcgtcaaggt ctttctggat ttctttctcc ttttcccgga gtgaaatatc
    15121 ctcccgttta cctgccgggg tcttatccgt aggcactaac ttccatgagt aaacaaactg
    15181 cggttttcca aaggtatcaa catatttgta agcatatcgt ccgtcttttc tctggctctc
    15241 tccagaccgc aaaacgcgat ttttgctgtc tcgtcttttt tctgacatct cgtcatgctc
    15301 ctttccatga cggaaagagc cgcatatatg cttataaatt attataacat atctgcggcc
    15361 ctttttcact attagattgt gtccaaagtg tcaattattt tctcaaactg cttgcgtttg
    15421 atctggatgc ggttgccatt cataatgacc cagccagagg ccggattttc ctcggccagc
    15481 ttacgcaatt tgttttctcc gatacggaaa tatttagacg cttcctcaat cgtcagcgtg
    15541 tatttttccc aaataggcac atcatggttc ataatgcagc cccctttcac aaattcaaag
    15601 gtttgtgtga tttaggggca tagacggacg gctgctggca cagctccacg ggactttcac
    15661 ccccgcgtgg ttctcacgcg gccctaccca ttgcctgcga cgcttttaac gctcggactg
    15721 tggctataag ggagtatcat agtcctgcct gtacgtcgtc gcccgcaagc cgccacgctc
    15781 acattgcggc gtggtgttcc tcgctccgct tttcccgtag ggaaaagtat catggcgcac
    15841 cagccgtccg gctcggcaca gacagaatgt gtccgtatgc cctatgatgc gccgccgtta
    15901 tcttgcgggc atatttatca aggtgcgatc aggccaccgg gaaaacaccg aaacggggaa
    15961 agggtaagcc gtttcatgct gccggggccg gttcgtcagt tcgtgacgat agcgtggatg
    16021 agcttgattt ccagacggcg cttcatggcc tcgtccagac acacatgcgg atagccgttt
    16081 tcgtcataga gcgtccgggt acacagcttg ttcatgtaac cgctgtaata ctgcaaaacg
    16141 cgctctacgg ctccggcatc cccagcgcgg gcggcctcga tcaccgaaag gggcaaaagc
    16201 tcaatgccct tgtagttcgg gttcttcatc cgcgtctaac ctcccttcgg catcaacgcc
    16261 tttagctgtt tccgcagctc cgataaggta tttgtcctgt gccgctgaac ggcactccgg
    16321 gacattccca caaggccgcc gatctcacca tctgccatat ccagaacaca gtgcaggatt
    16381 aaaatactct gctccatgct gggcagcgcg gcaaaggctt cggcaacaag ctcattgttg
    16441 atatgtaggt catagccatg cgacgaaaac gtaaagctgt cgctggggta gtgatctact
    16501 gtacagagct tgtccaaatc tgcctgtgac aaagagctta gtgacacttc acggtcacgg
    16561 cggcgcttca tttcagccaa gtagcttttg gcctcgttac gcagtaccgt cttgcaaaaa
    16621 gcatcgaaca tgcaccgcgc ttcatagtcg cgggggatag catccatctt ctcaccccct
    16681 ttctgtgggg gacttaggtg gttctctccc tttccgctaa caagactttg acaaggccgc
    16741 cagctacccg ctaaatacct cctttttgaa aatttctcaa aaaatttttt tggggacagc
    16801 cgggccgcaa aagacgacaa aaatcgcccg gcaggtcgca aacctaccgg gcgagaaaat
    16861 gctttgctat gctgctgatt tacatggtat agtacatact cataaccgga atagccctgt
    16921 g
//
LOCUS       DAZUMI010000061        16505 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000061 DAZUMI010000000
VERSION     DAZUMI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.061"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..724)
                     /locus_tag="ACG81C_001720"
     CDS             complement(26..724)
                     /locus_tag="ACG81C_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5064875.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(717..2069)
                     /locus_tag="ACG81C_001721"
     CDS             complement(717..2069)
                     /locus_tag="ACG81C_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5064876.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2292..3692)
                     /locus_tag="ACG81C_001722"
     CDS             complement(2292..3692)
                     /locus_tag="ACG81C_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5064877.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3853..4590
                     /locus_tag="ACG81C_001723"
     CDS             3853..4590
                     /locus_tag="ACG81C_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5064878.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4715..5194
                     /locus_tag="ACG81C_001724"
     CDS             4715..5194
                     /locus_tag="ACG81C_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5064879.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5191..5958
                     /locus_tag="ACG81C_001725"
     CDS             5191..5958
                     /locus_tag="ACG81C_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5064880.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6188..6757
                     /gene="lepB"
                     /locus_tag="ACG81C_001726"
     CDS             6188..6757
                     /gene="lepB"
                     /locus_tag="ACG81C_001726"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5064881.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6861..7463)
                     /locus_tag="ACG81C_001727"
     CDS             complement(6861..7463)
                     /locus_tag="ACG81C_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5064882.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7569..7799
                     /locus_tag="ACG81C_001728"
     CDS             7569..7799
                     /locus_tag="ACG81C_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064883.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7790..8038
                     /locus_tag="ACG81C_001729"
     CDS             7790..8038
                     /locus_tag="ACG81C_001729"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064884.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8144..8728
                     /locus_tag="ACG81C_001730"
     CDS             8144..8728
                     /locus_tag="ACG81C_001730"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5064885.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8731..9804
                     /gene="prfA"
                     /locus_tag="ACG81C_001731"
     CDS             8731..9804
                     /gene="prfA"
                     /locus_tag="ACG81C_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5064886.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9797..10636
                     /gene="prmC"
                     /locus_tag="ACG81C_001732"
     CDS             9797..10636
                     /gene="prmC"
                     /locus_tag="ACG81C_001732"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5064887.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10629..10808
                     /locus_tag="ACG81C_001733"
     CDS             10629..10808
                     /locus_tag="ACG81C_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064888.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10830..11855
                     /locus_tag="ACG81C_001734"
     CDS             10830..11855
                     /locus_tag="ACG81C_001734"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5064889.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12046..13290
                     /gene="glyA"
                     /locus_tag="ACG81C_001735"
     CDS             12046..13290
                     /gene="glyA"
                     /locus_tag="ACG81C_001735"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5064890.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13443..14390
                     /locus_tag="ACG81C_001736"
     CDS             13443..14390
                     /locus_tag="ACG81C_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5064891.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14524..15153
                     /gene="upp"
                     /locus_tag="ACG81C_001737"
     CDS             14524..15153
                     /gene="upp"
                     /locus_tag="ACG81C_001737"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5064892.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15292..15534)
                     /locus_tag="ACG81C_001738"
     CDS             complement(15292..15534)
                     /locus_tag="ACG81C_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064893.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15655..15727
                     /locus_tag="ACG81C_001739"
     tRNA            15655..15727
                     /locus_tag="ACG81C_001739"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15688..15690,aa:Thr,seq:cgt)
     gene            complement(15846..16484)
                     /locus_tag="ACG81C_001740"
     CDS             complement(15846..16484)
                     /locus_tag="ACG81C_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5064894.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5631 a         2947 c         3318 g         4609 t
ORIGIN      
        1 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
       61 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
      121 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
      181 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
      241 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
      301 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
      361 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
      421 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
      481 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
      541 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
      601 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
      661 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
      721 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
      781 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
      841 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
      901 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
      961 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
     1021 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
     1081 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
     1141 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
     1201 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
     1261 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
     1321 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
     1381 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
     1441 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
     1501 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
     1561 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
     1621 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
     1681 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
     1741 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
     1801 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
     1861 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
     1921 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
     1981 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
     2041 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
     2101 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
     2161 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
     2221 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
     2281 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
     2341 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
     2401 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
     2461 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
     2521 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
     2581 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
     2641 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
     2701 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
     2761 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
     2821 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
     2881 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
     2941 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
     3001 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
     3061 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
     3121 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
     3181 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
     3241 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
     3301 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
     3361 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
     3421 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
     3481 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
     3541 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
     3601 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
     3661 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
     3721 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
     3781 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
     3841 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
     3901 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
     3961 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
     4021 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
     4081 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
     4141 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
     4201 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
     4261 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
     4321 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
     4381 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
     4441 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
     4501 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
     4561 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
     4621 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
     4681 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
     4741 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
     4801 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
     4861 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
     4921 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
     4981 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
     5041 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
     5101 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
     5161 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
     5221 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
     5281 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
     5341 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
     5401 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
     5461 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
     5521 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
     5581 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
     5641 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
     5701 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
     5761 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
     5821 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
     5881 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
     5941 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
     6001 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
     6061 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
     6121 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
     6181 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
     6241 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
     6301 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
     6361 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
     6421 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
     6481 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
     6541 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
     6601 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
     6661 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
     6721 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
     6781 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
     6841 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
     6901 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
     6961 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
     7021 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
     7081 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
     7141 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
     7201 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
     7261 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
     7321 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
     7381 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
     7441 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
     7501 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
     7561 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
     7621 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
     7681 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
     7741 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
     7801 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
     7861 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
     7921 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
     7981 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
     8041 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
     8101 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
     8161 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
     8221 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
     8281 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
     8341 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
     8401 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
     8461 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
     8521 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
     8581 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
     8641 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
     8701 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
     8761 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
     8821 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
     8881 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
     8941 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
     9001 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
     9061 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
     9121 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
     9181 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
     9241 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
     9301 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
     9361 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
     9421 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
     9481 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
     9541 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
     9601 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
     9661 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
     9721 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
     9781 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
     9841 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
     9901 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
     9961 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    10021 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    10081 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    10141 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    10201 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    10261 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    10321 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    10381 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    10441 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    10501 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    10561 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    10621 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    10681 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    10741 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    10801 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    10861 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    10921 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    10981 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    11041 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    11101 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    11161 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    11221 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    11281 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    11341 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    11401 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    11461 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    11521 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    11581 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    11641 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    11701 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    11761 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    11821 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    11881 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    11941 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    12001 tgtaggaaaa taaaattgta ggaaaataaa aaggagctgt tgtttgtggt agattacaaa
    12061 acgtttgacc ctgatctttg ggctgcgatc gccaaagaag aagaaagaca agaacataat
    12121 ttggagctga ttgcctctga aaactttgtc tccgaagcag tgatggctgc acaaggaagt
    12181 atcttgacaa ataaatacgc agaaggttat ccagggcatc gctattatgg tgggtgtgaa
    12241 tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa
    12301 tttgcgaacg tccagcctca ctccggctct caagccaaca cggctgctta tctcgctttg
    12361 gtagaaccag gggacacgat cttaggaatg gatctttccg caggcggaca tctaacacat
    12421 ggatcaccag tcaacttcag tggcaaaacc tatcactttg ttgcttacgg tgttgatccg
    12481 acaacagaag ttattgatta taatgtggta cgtattttag ctagaaaaca tcaaccaaaa
    12541 ttgatcgtag caggtgccag tgcttacgga cgaacgatcg actttgcgaa attcagagaa
    12601 atcgcagatg aagtaggcgc aaaactgatg gtagatatgg cacatatcgc aggattagta
    12661 gcagcaggac ttcatcctaa tccagttcct tatgcagata tcacaacgac taccactcat
    12721 aaaacattgc gtggccctcg aggcggcatg attttgacaa acgatgaagc attagcaaaa
    12781 aaaataaata gtgcggtatt tcctggtatt caaggcggcc ctctagagca tgtgattgcg
    12841 ggtaaagcag tagcattcaa agaagcatta gatcctgcat ttaaagaata tagtgaacaa
    12901 atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg aaccagagtc
    12961 atttcagggg cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat
    13021 ggaaaagaag cagagagcat tttggacagt gtgaatatca ctgtaaataa aaactcgatt
    13081 ccttttgaaa cactaagtcc attcaaaaca agcggtattc gaatcggcac gccagcaatc
    13141 actactcgcg ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca
    13201 ttacaagcaa aagatgataa cgctcaacta gacgaagtca aaacaggtgt acgcgaatta
    13261 actgaaaaat ttccgttgca taaaaaataa aaatttaaat tccattagta aacagaaaag
    13321 ttcaatgttg ggcttttctg tttatttttt gggaaaacgg attctttgac aatatcggtg
    13381 atgctctcta gtatatgagg tgtaatttta gtcttcacac taaatctcag ggaggaaaag
    13441 tcatgactga tcgtatctcg ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc
    13501 ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag agttttacta gaagaagtac
    13561 aagaatcacc tgtgaacaaa tattccgtcg atattgaaga cttgatggtg gatactggcg
    13621 aatggggaaa actcaatatt cgtctcgtac gaccagaagg gaatatggat aaattaccag
    13681 ttatttttta tattcatgga gcaggctggg tttttggcag tgctcaaacc catgataaat
    13741 tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat
    13801 caccagaagc aaaatatcca acagcaattg aacaaaatta tgctgtgttg caacagctga
    13861 ataaattagc caagtcaaaa aatctggatc tcgagaatct aatcgttgca ggagattcag
    13921 taggtggtaa catggctaca gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa
    13981 tatccaagca attgctgtat tatccagtca caaacgctga ttttgataca gattcctatc
    14041 atgaattcgg agaaaactat tttttgacaa gagaaggaat gatgtggttt tgggaccaat
    14101 atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa
    14161 atgaactaaa tgatcttcct tcagcaatga ttttaacggg agaagcggat gtattaagag
    14221 atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac
    14281 gcttccaagg aatcatccat gattttgtca tggtaaatgc tatggatcag acaaaggcaa
    14341 cgcgtgcagc aatggcatta tctacttctt ggatcaagaa aaaagaataa gctttgcttg
    14401 tccgcaggaa aacttctttt caaaaacgga ttttcttagg aaaaatggcg agtttgacta
    14461 gtcaatcact tgattttgtt ttacaattaa aaagaaacga cagaacaaag gagagcttaa
    14521 aaaatgggca aatttcaagt gatcgatcat ccattgattc aacacaaact aactattatt
    14581 cgggacaaaa actgcggaac aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg
    14641 atggcatatg aagtatcacg ggatatgcca ttggaagaca tcgtgatcga aacaccgatc
    14701 actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag cgattattcc aatcttgcgc
    14761 gcaggtattg gaatggttga cggtatcttg gaattgattc cagctgctaa agtgggacat
    14821 atcggtcttt atcgagatga agaaacattg gaaccacatg aatatttcgt caaacttcca
    14881 gaagacattg atgcacgtca gttgtttgta gtagatccaa tgttggcaac aggcggatca
    14941 gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt
    15001 ttggtagcag cacctgaggg agtaaaagct ttgcaagaag cgcatcctga tattgacatc
    15061 tatacagctt ctctagatga aaaattgaat gaacatggtt atatcgttcc tggactaggg
    15121 gatgcgggtg accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa
    15181 cttttgtcac atcctttttt ggtcattttt aacgtttgat gcagaacagc tttttcacag
    15241 ctttcgtttg catataaaaa aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt
    15301 ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca agttcgcatt
    15361 ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg aaatcgttcc
    15421 agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga atttttcacc
    15481 aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc
    15541 tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta aactaatttt
    15601 ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga taaggccagc
    15661 ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc gatccctata
    15721 gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga tacatctgtc
    15781 tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat tatttctata
    15841 aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat cacatgaact
    15901 gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa caatcttgcc
    15961 accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt atcataagta
    16021 ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg aatgctgaac
    16081 ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag tatcatgata
    16141 ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc tgttaaaccc
    16201 catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac attgataaaa
    16261 aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt tttcgtatct
    16321 tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga aaaaatgcta
    16381 ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat agtgcccaat
    16441 atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat aactcctact
    16501 attca
//
LOCUS       DAZUMI010000062        16494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000062 DAZUMI010000000
VERSION     DAZUMI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.062"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG81C_001741"
     CDS             <1..546
                     /locus_tag="ACG81C_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5064895.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="ACG81C_001742"
     CDS             735..1403
                     /locus_tag="ACG81C_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5064896.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1923)
                     /locus_tag="ACG81C_001743"
     CDS             complement(1603..1923)
                     /locus_tag="ACG81C_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064897.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1960..3342)
                     /locus_tag="ACG81C_001744"
     CDS             complement(1960..3342)
                     /locus_tag="ACG81C_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5064898.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="ACG81C_001745"
     CDS             complement(3498..4685)
                     /locus_tag="ACG81C_001745"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5064899.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="ACG81C_001746"
     CDS             complement(4692..5723)
                     /locus_tag="ACG81C_001746"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5064900.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="ACG81C_001747"
     CDS             complement(5769..7193)
                     /locus_tag="ACG81C_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5064901.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7368..8273
                     /locus_tag="ACG81C_001748"
     CDS             7368..8273
                     /locus_tag="ACG81C_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5064902.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="ACG81C_001749"
     CDS             8364..9041
                     /locus_tag="ACG81C_001749"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5064903.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9038..9208
                     /locus_tag="ACG81C_001750"
     CDS             9038..9208
                     /locus_tag="ACG81C_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064904.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9322..10218
                     /locus_tag="ACG81C_001751"
     CDS             9322..10218
                     /locus_tag="ACG81C_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5064905.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="ACG81C_001752"
     CDS             complement(10273..11643)
                     /locus_tag="ACG81C_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5064906.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="ACG81C_001753"
     CDS             11881..12627
                     /locus_tag="ACG81C_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5064907.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="ACG81C_001754"
     CDS             12624..13463
                     /locus_tag="ACG81C_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5064908.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="ACG81C_001755"
     CDS             13464..14414
                     /locus_tag="ACG81C_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5064909.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="ACG81C_001756"
     CDS             14555..15307
                     /locus_tag="ACG81C_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064910.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="ACG81C_001757"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="ACG81C_001757"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5064911.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5367 a         3084 c         3317 g         4726 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac atta
//
LOCUS       DAZUMI010000063        16211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000063 DAZUMI010000000
VERSION     DAZUMI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.063"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..468
                     /gene="scm"
                     /locus_tag="ACG81C_001758"
     CDS             <1..468
                     /gene="scm"
                     /locus_tag="ACG81C_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5064912.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(623..1609)
                     /locus_tag="ACG81C_001759"
     CDS             complement(623..1609)
                     /locus_tag="ACG81C_001759"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5064913.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1632..2198)
                     /locus_tag="ACG81C_001760"
     CDS             complement(1632..2198)
                     /locus_tag="ACG81C_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5064914.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2212..2628)
                     /locus_tag="ACG81C_001761"
     CDS             complement(2212..2628)
                     /locus_tag="ACG81C_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5064915.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3085..5019)
                     /locus_tag="ACG81C_001762"
     CDS             complement(3085..5019)
                     /locus_tag="ACG81C_001762"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5064916.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5321..6256
                     /gene="pplA"
                     /locus_tag="ACG81C_001763"
     CDS             5321..6256
                     /gene="pplA"
                     /locus_tag="ACG81C_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS5064917.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6346..7449
                     /locus_tag="ACG81C_001764"
     CDS             6346..7449
                     /locus_tag="ACG81C_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5064918.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7462..8415
                     /gene="menA"
                     /locus_tag="ACG81C_001765"
     CDS             7462..8415
                     /gene="menA"
                     /locus_tag="ACG81C_001765"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5064919.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9229..10575)
                     /gene="gor"
                     /locus_tag="ACG81C_001766"
     CDS             complement(9229..10575)
                     /gene="gor"
                     /locus_tag="ACG81C_001766"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5064920.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10709..11962)
                     /locus_tag="ACG81C_001767"
     CDS             complement(10709..11962)
                     /locus_tag="ACG81C_001767"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5064921.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11968..12891)
                     /locus_tag="ACG81C_001768"
     CDS             complement(11968..12891)
                     /locus_tag="ACG81C_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5064922.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13112..15604)
                     /locus_tag="ACG81C_001769"
     CDS             complement(13112..15604)
                     /locus_tag="ACG81C_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5064923.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15611..16078)
                     /locus_tag="ACG81C_001770"
     CDS             complement(15611..16078)
                     /locus_tag="ACG81C_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5064924.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4763 a         3390 c         2881 g         5177 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
      121 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
      181 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
      241 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
      301 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
      361 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
      421 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
      481 gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa
      541 tacaagaact acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt
      601 tacgtatcga acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa
      661 agagattctc ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct
      721 tttctgtgta agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga
      781 ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta
      841 gtagctttgg tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac
      901 gcatgtcttc tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat
      961 catcgataat ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga
     1021 agcgctcgct tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag
     1081 ctgtcttacc tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat
     1141 catccatctg ccctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga
     1201 gacttttcaa ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt
     1261 ctcctgcata gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc
     1321 gcagatccgc ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga
     1381 tagaagctgc caaagcgatg gctttgtctc gatcttgctc tggtccaaat tgtgagaaca
     1441 atagctgata ggcaggacgc aacagctttc cgccggcatg gatcgtttct ttgattgctt
     1501 gttcgacagg tttgtttttc aggcggacag tttcttccat ccgttttagt gtttctgtca
     1561 attctttact taaagcagga tagttttccc acattggatg tagcttcata aaaaaactcc
     1621 ttgtgtgacg attatttttt ggtaaacatt gggctccatt tttgtttggt tccctgctca
     1681 taatcttttt ggaatcgttg aagtgtctgc acatgtcgta aaagataatt cgctgcgatc
     1741 ccgattgcaa tccctgataa aatccctaaa actgaaagga ctggcaagta caacatgact
     1801 gtccatgatt gtgcaatgaa agaagccatc aaaagctgtc cgacattatg catgaaaccg
     1861 ccggccgcac taattccgat gatactgaca cgttttggtc ctaattgctt gaccaataac
     1921 attccaaaat aactaagcaa agcaccactc atactataaa ggaatgtgga aatcgttccg
     1981 cctagcaaag tagtcaggaa tagtcgtagc caaagtagta agaagctgtc ttgttttttc
     2041 atggtaaaaa tggcaacgat cgtgattaga ttggctaagc ctagttttgc accaggcgca
     2101 aaagaaaaag gataagggat catattttcc aataaaccaa tgatcactcc ttgtgcaaca
     2161 agtagcgaaa tataaatcat tttttgtaat ttactcatgg tgtgtaaagg tctaataaat
     2221 cagactgtct tcgtcacctc catctgttga acggacttcg atcactaatt tatgtggcaa
     2281 acagacgatg gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga tctggtcatc
     2341 gcaatctgct tcttttatcc gtactcgatc gccatctatc tcgatcaagt tataatctcc
     2401 gtgttcatct ttgtaagtat atgtgttgct ctcttctcca gccatcaaag ggaatgtttt
     2461 gatttctgtg ccatccacac gcagtaccgc ttctttttct gctgttgcgg tgttttgctg
     2521 aagaccaaaa atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa taataaaaac
     2581 atcccaaggt ttgagcagac ttttttcagc aaattcttta aggctcattt ttgttctcct
     2641 ttttccagat taactcaccc aattttagca taaaaacaag aggttgtgac acctgtgtct
     2701 tgctttgagt aataagaacg aagaatagaa aacagctttc catgttttct aattctttgg
     2761 gcttattacc gagaagcagc aggtggaaca ccgtttataa caagaggttg tgacagaagt
     2821 gggcaacttc aagaaataag aaggaattta cgaaaattac ttttcaagtt tttgtgaaat
     2881 ttcggcttat ttttcgaaga agctacttct ggaacaccgt ttataacaag aggttgtaac
     2941 agaggttgtg acacctgtgt cttgctcatg gaggcttatc tcgggcatct tattccttta
     3001 attttacata aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt gaaatcttgc
     3061 attcaaataa cgttctttat ctatttattt cttgccgtac tgggaacttg gtctaccgta
     3121 ccaccaatta cctagtgtac gttgtagcca tggaattacc cagcgatcca acccaatcgc
     3181 acgtccggaa ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt ttacccagta
     3241 aaacatgcct gataagcaga acgtgatcac caatacaatc gttgtcgcac tgcatagcca
     3301 tgtgaataat ccagcaatca atgctaatgc caaggcaatt tcaaagaata ccatgaattt
     3361 ttggaagaac attgctacat cattgtttgg catgatcact tgcatgatct ttgtgaacca
     3421 gtctggtgac ttctcaatca cagccattgg ttcttcaccg tatgcataac tcaaaccaaa
     3481 atgtgcagct tgagtcgctg cttctccggc acttgcccct gctccgctgg cagctgtggt
     3541 tgcatcacca gcagcagaag caccactggc aacatctgtt acattgtatt cccatgtcca
     3601 agggaacaca ctgtttccag tgaaccatga tccttcacca aaccaagtac ttggtttgaa
     3661 cacttcacca ttcccaatga tttttttgga agcttcaacg aaccaaacta aaccatagaa
     3721 aatacgaagt ggcacactcc ataagacatt gccgtaacga gaagaatgtc ctcgagtcac
     3781 gttgcgatca tctttgatat ggaaaaactc atgcattaaa tattggaaca tataataacc
     3841 agaacgaata tcaaagaagt atttcaaatt cacgatatgt ttcattacga tagctaaaaa
     3901 tccgcttaaa tggatcttat caaataggtt agctaccccc catttggcac caatagaaac
     3961 cataaatcct tgatagtttc ctttaaattt atgcttttct ccgcctttga tcgaagccac
     4021 gatattcgct gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct gcggtgtcgg
     4081 tgttcctggg aattcttcat aaaagactaa atcaccaatg atatagatgt ttttatcttc
     4141 gtagcctttt gcctgcatat attcattggc gatcaaccgc tgtccgcgtg ctgattctaa
     4201 tccaaagtca gcagcgtcag aagttccttt gacaccagct gtccaaatca gtgtgtgagt
     4261 cggaatgttg cgtccgtctt tcaatttgat atgatcagca gccacttcta cgattggtgc
     4321 attcaataat aattccacgt tgtgcttacg caaatagcgt tcagcttttg cagcatcatt
     4381 tcgatccaac atattcaaga tcgtcggcat tgcctctacg accatcagtg tgatttcgtt
     4441 tggatctaat ttataatctt tggctaggcg atttttccag tcgatcagtt ccccaatcat
     4501 ttcaataccg gtaaatccag aaccacagac aacgaaagtc agcatggctt tacgcagttc
     4561 atcatctggt tccaatgcag cttttgctac tgtcttctca atatgttccc gaatccttac
     4621 agaatcttca aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg taccaaagtc
     4681 attcggctct ccgcccattc caaggatcag ctgatcaaaa ggataggaac ctaatttggt
     4741 cttgacgatt tttcgttctt tatcgatacc gattacagtg tctgtgacaa gttgtacatt
     4801 cttttgacga gcaaacaaat gctgcagatc gtattggatt gcttctggtt ctacacgtcc
     4861 gccagctact tcatgcaact cggtcatcat agtatggtac gaatgacgat cgatcagagt
     4921 aattgctaca tcttcttttt tcagtttctt tgccaagtat tttgtagctg aaacaccggc
     4981 atatcctgct cctacaacga caatattttg ctttgccatt gatcatccgc tccttataga
     5041 ctatctatat aatgaatata tgataattct tttagtttga tacttattac acaatctcca
     5101 tatattatat agatgttctg ttcttttgtc taactccttt tggatttttt tcaaatttta
     5161 aaataaagcg ttttttcacg aaatttgctg aaagaaaacg tttgaaatat tacatgggtt
     5221 atttatcaaa aatagttgac ttataggcta aaaagattag aatgtataat gaatcacaaa
     5281 gaaaaattgt ggactagaaa tggaatggtg gggtattaaa atgaagaaaa aacgttttgt
     5341 aactggattt gccgtattag cattttcagc tctagtgtta ggcgcttgcg gcgcagatga
     5401 caacggaagc tcaaactctt cttctgaatc atctacagca caatcttcga ctgcaaaaag
     5461 cacaacagaa tcatctgcaa aagttgtagc tggcggagat ttgcaagacg gcacatataa
     5521 attagaagaa aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg ttgtaaaaga
     5581 cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa aacggcgaat caaaaacaaa
     5641 agatgcagaa tacaacaaaa acatggaagc aaaatctggc acaaacccag aaaaatacat
     5701 tcctgagttg aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg aagtcgtaac
     5761 tggagctact cactcatctg aaagcttcca aaactacgca caacaattga tccaagctgc
     5821 tcaagctggt aacacagata caatcgaaat cgacaatggt gctgatttga aagacggcac
     5881 ttacaagctt gaagaaaaga actactctaa cggctaccgt gtgcaattcg aaatgacagt
     5941 tgctggcggc aaagtaactg aatcaaactt tgattatatc gacaaagacg gtaaatcaaa
     6001 acaagatgat acagaatata atgaaaatat gaaagcaaaa agcggtacag aaccaaaaac
     6061 ttacatccct actctgaacg acgaattcgt caaagcaatg ggcgaagaag atggctcacc
     6121 tgctaatgta gaagttgtta caggtgctac acacagctca cattcattca tcatgtacgc
     6181 acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag tagataatat
     6241 cgtaacagaa aaataatcac ataatcatac taataaaagt tggagggtgg gacgatagcc
     6301 agattaggca gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg gaaaaaacta
     6361 ctattttcct taatcacact gggcatctct tttatggtgc taactggctg cagcaactca
     6421 gcaaataaaa caaccactga aagttcagca gctaccaaga tattgaaaga tccatattct
     6481 gatgaacagt tcttgttagg gacttacgtt cgtatccgtg tgtatgatga aggcaaagaa
     6541 tcagcattga aacctgcctt cgatcgagta aaagaacttg gtgataaaat cacgatcaat
     6601 caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc agtaaaagtt
     6661 tctgatgacg tctacacgtt agtcaagcgg gcctacgaat atagccaaga ttctcaaggc
     6721 ggattcgata tggcgattgg tgcaatcaca caattatggc gaatcggttt tgatgacgcc
     6781 agaaaacctt cccaagaaga aatcgatcag gcattgaaat tagtggatta tcataagatc
     6841 gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat catcgattta
     6901 ggtgcaatcg ccaaaggtta cattacggac gaagtagtga aagtcttgaa aaaacaagga
     6961 gtgaccactg ccatcgttga cttgggcggt aatgtctatg tcttaggcca cagcccaaga
     7021 ggcgaaaacc aagactggac agtcggcatc caagatccga acatggctcg cggatctgtt
     7081 ttaggttcta ttaaagaacg gaacaaaaca ttagttacct ctggaattta cgagcgttat
     7141 ttagaagtag atggcaaaaa ataccatcat ttgtttgatc caaaaacggg ttatccgttc
     7201 gacaatgata tcgcaagtgt cacgatcata acagataaat caattgatgg tgacgggttg
     7261 tctacagcag tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga aagcgaattg
     7321 aataacggga cggaagcgat cttcgtgaca aaagacgata aagtatatgt gaccgatcca
     7381 atcaaagaca ccttcaaatt atcggaggac tcccattaca cgatgggtga ccgttctgaa
     7441 ctaaaataag gagtacgaac catgacacta aaaatctttt tagaagtagt tgaaatacga
     7501 acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat tattctctat cgcttttttt
     7561 catgagatac attggctaaa cactttttta ttttttattg gaatgctagt ttttgattta
     7621 gcgactaccg ctatcaataa ttacatggat tatgaaaagg ctcattctga agtatataag
     7681 tatgaagaaa atgtcattgg ccgcagaaat ctatctcctg tattgattcg aaacatgatt
     7741 ttagctatga ttgcatttgt ttttgttatc ggcgttattc taacgatcaa tacgggttgg
     7801 cttttacttt tgatgggatt tatctgttgt tttattggag tattctatac attcggaccg
     7861 attcctttat ctcgaatgcc tttaggggaa attttcagtg gggtgactat gggccttgga
     7921 atttttgcca tggtcgttta tgtcaataca ttcggttcag atgtctatga tctgacagtc
     7981 aacttttcat ctggacaatt tgctttggta ggaaatcttt gggctatagc agcaatcgta
     8041 ttagcttcct tacccttggt gtttacgatt gccaatatca tgttggctaa taatttgcgg
     8101 gatcttgacc gtgatattga aaatcatcgc tatacactag ttttttatat cggacgtcct
     8161 gcgggaacag tcttattcca gctattgatg tatgcttgct atttcgtgat tttaatgggg
     8221 ttgttgagcg gtatctatga atggccgatc ttggtcacct tcttgacctt gccaattgtg
     8281 aagaagcatt tggatcaatt caagcaaacg ttaccccagc ctatcagttt cagctattcc
     8341 atcaaaaata tggtcttatt caatgccagt tatgtattgg ggctattact aacaatcatt
     8401 atgcaagcca tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat caagtaataa
     8461 gaacagccaa ataaaaatag ctccttatat ttttcgtttg gttgggctta ttaccgcaga
     8521 tgcaagcttt tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag tttcgagtat
     8581 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
     8641 gaaactggct tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt gtcctgagta
     8701 ttagagaaac aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga
     8761 aggacagctg attgaacact atttgttcgg aaaaagtgac taactatgac acaatttttt
     8821 tcacagtccc ttttttcact tgatttgatt cgtaccagcg aacttgacga gaactttctt
     8881 ggtttggtaa ctatatggta ttcttttaac atcctcgttt gcttcttcta atttatttat
     8941 acaaagtcac acttttttaa acatctgttc ttcgatacat ttctatttct tttaaacatc
     9001 gataccaata ttttgaactt aagagtgcat accaaatgga ttcatttact cttcgtcagt
     9061 tacgaagata atcaaaatct ccaacttccc tacttatttc gttttgagaa ttatcgaagc
     9121 aatcttacat aaataactaa aaaacaaaaa accagaaaat cagtatactg attttctggt
     9181 tttacatggt ttgttcaata aaaagctgtt ggaactgtta atattaaatt atcgcatcgt
     9241 tacgaattct tccgctcctg ttggatggat ggctactgta ttgtcaaagt cttctttagt
     9301 tgcacccatc ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac caacaccaat
     9361 agcatgtaag ccgacgattc tttcttcttt tccgacacag atcaatttca tttcgcattt
     9421 ttggcgataa tcatttaatg caaagtacat tggggtaaag cgtgaacgat aaactttcaa
     9481 ttcattttcg ccatattttt ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg
     9541 tggatgcgta aagacgactg tcggaacaag tttatagtca agataagaat ctttctttcc
     9601 attaaataaa cgttctgata aacgtctacc agctgcgatt gcaacaggag tcagatctaa
     9661 tttaccgatc acatcaccaa cagcaaaaat atgttcatcc gttgtatttt ggtatttatc
     9721 tactttgata aacccttttt tgtccaattc cacatttgta ttttccagac ctaatttttc
     9781 ggtattcggt acacgtccac cggcaaaaag tacacaatca ccagttaagg tttcaccatt
     9841 ttcaaatagc accgtatatt cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc
     9901 gtaagttttt attccagctt cttggtacat ttggatcaaa ttgtccgaaa gcattttatc
     9961 aaacgttcta agtggtcgtt cttttcgata tgcccacatg acttcactac ctaaaccacg
    10021 aaatacacct gaaagttcag ccgcaatgta tccagcacct aaaacgattg ctctcttagg
    10081 taactctgtt aattcaaaaa atccatttga atcaattgcg tattctccac caggaatggt
    10141 cattttactt gcacggcctc cagtagcgat caagatttta ggagcacgat aggtttcacc
    10201 gtttacttcg atcgtttgac tatcgataaa agtagcgtaa ccttttatag cctcgacatg
    10261 attgctatct aatcctcttt gataagcacc gtgtagaaaa tcaatatatt tttctcgtct
    10321 ttctactaga ccggcgaaat caacattttt taaatctgct tggataccgt aacttgaggc
    10381 atctcgttca attgtttcca aaatcgtact tgcttgccac ataacttttt taggtacaca
    10441 accgacgttg acacatgttc cgcccaattc attcgcttca atcagcaaaa cttttgcgcc
    10501 atgcatccct gctctgtttg ctgaagcaat accgccagag ccgccaccaa ttacgatgta
    10561 atcatatatt tgcattttgt tcctccttat tgaagagatc aatcaaaacc tttatttaac
    10621 gatccttttt ctacagaaaa caagtgtaac acttttcttg aattttgtat gcgaagatgc
    10681 ttaaaacagg gatatatcag ctttttcctt atttacgttt tcggatcata tctccaggtt
    10741 tcaccggctg tgcgactggc atagttaaaa tcatcattgg atttggtgct cggtcaatgg
    10801 attcgccttc ttcattatgc ataacttcaa cggtttgact gaaatgactg aatcctggac
    10861 cataaaattc gatttcatct cctacagaaa acaagtttct ctgacgtatc gtagcaatct
    10921 gtgttgcctc atcataagat aatacttcac cgacaaattt atatactggg attttacgac
    10981 gttcaccgaa taattgttcg ttttcagttg gtgtattgta gtaaaaacct gtagctaatt
    11041 ctcgttgtgc tactttccat aactcgtcga tccattcttg cttgcagaca tagttttcag
    11101 gatcttccat ataactgtct acagccgctt tatagacatt tgacaccgta gatacataat
    11161 ggatcgattt cattcttcct tcgatcttga agctgtctac gccgttttca atcaattctg
    11221 gaatatgttc gatcattgac atatccactg cactcatcga gaattcttct tccacttcgc
    11281 ctgtatcagt taaactgttt cgttcttgac caaaaggcat atcgaacaat ccatacttcc
    11341 agcgacaaga ttgagaacat ccgccacggt ttgcatcccg catcgacata tggttggaca
    11401 atgtgcatcg accagaataa gagatacaca tggcaccatg gataaatgct tcgatttcgg
    11461 tatccgtatt tttacgaatc tctgccactt cctccattga aacttcacga gctaaaacga
    11521 cacgttccaa cccttcttct ttccagaatt ccaatgtctc aaaattagtt gctgaagctt
    11581 gagtggataa atggatcggc agacctgggg cttctgtcgc acaaatttcg atcaaagctg
    11641 gatcagaaac aatcactgca gaaataccga catcacgcaa actgcggaaa aattcccctg
    11701 ctccatcttc atttccttca tgggtgacca tattagcagc aacgtatacc ttcgcatcat
    11761 gttcttttgc aaaagcaact ccttcagcca tttcttcata agtaaaatta cctgcacggc
    11821 ttcttaaacc atatgcattc ccgccaatat atactgcatc tgctccataa tggatcgcgg
    11881 tttttaactt ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc aattttcttt
    11941 ttactgtcat ttctttcatc atactcctta tttgatagca gaaggatcaa tataaaagaa
    12001 tcctgtatct aattcacggt tttcaggatg caatttactg atttcgctag tcattttttc
    12061 cgctttttct tgatcccagc taccttctaa aatttgtttt tttgcttcgg cgaatagtcg
    12121 agtgatgttt acgaattttt cgcctggagt aaataatccg tccaatttcc atgtacagta
    12181 atgatgctga taaagttcat tcaattcatt cgagagattc agatcattgc tggcaaaaat
    12241 atgtgtgcca tgactatctt cataaataga ataatgtgtc tcttcttttt taggttccga
    12301 gataaaaaga cctctttcac gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt
    12361 ctgtagcagt ggacgtttgg attgatggat gcatgtagca ccatacacaa gggtttctac
    12421 aggaatatcg agtttttctt ccatcgcctt catctcttca aaaggaactt cacgagcaag
    12481 tactgctcca actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa
    12541 tgtttcagca tcataaatat acggtatacg caattcagga ttttttttca tcgtataaat
    12601 aatacctgta tcaccaactg tgatcacatc tacgtttatt tcttttaaaa aagccaaata
    12661 ttcaggtaca tttttcattt tttcaggatg aatgatccca tttacagcaa tattcgcttt
    12721 tttaccatac ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc
    12781 aggcagacgc aatccaaatt gctcttcacc gaaatatagt gtatctgcac ctgcagcaag
    12841 caactgttct gcttgttcaa tagtttctac tgtggttatg agttcgatca taattttctc
    12901 ctcctacaac ttcataatag tagcatagct ctaaaaactt tccaatactt cttctattta
    12961 aagtcggatt tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat atattctgtc
    13021 caaatataaa aagagattgt gacaaaaatc ttgtcacaac ctcttaatca agtcttacta
    13081 ttataaaaat tcagctacac aggccaagta actataggga acctactgtt tcctttttag
    13141 gtccgcgaac attcaatgtg atcttacctt ttgaagcacc tactgttacg tggtcaccta
    13201 aacgaatttc tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg
    13261 cgcgacggat tggacgtgca ccatattcag gatcaaatcc agctttcccg atcacatcga
    13321 ttgcagatgg tgtgattttc acttgaatat cttgttcttt caatcgtttg ataatcgctt
    13381 tgctcatgat ttggacgatt gtatggattt catctttact caatgaatgg aagacgacag
    13441 tctcatcgat tcgattcaag aattcaggtc ggaaagcttt ctttaactct tctaagatac
    13501 gtttttgcat ggctttgtga tctttagtaa catcttggac attgaatccg acattttttt
    13561 cttctcgaat ttgtgtcgct ccgatatttg aagtcataat catgattgta tttctaaagt
    13621 ccacttttct acctttagaa tctgttaaat gaccatcatc caatacttgc agaagtaaat
    13681 tgaaaacgtc tggatgtgct ttctccactt catctaagag aatcactgaa tatggttttt
    13741 gacgaatttt ttctgtcaac tgtccacctt catcataccc tacgtatcct ggaggagacc
    13801 cgattaatcg gcttgtactg tatttttcca taaattctga catatcaaca cggatcaggg
    13861 catcttcact tccaaacatg acttctgaaa gtgctttggc caattctgtt tttccgacac
    13921 cagttggtcc aaggaacatg aatgaaccaa ttggacgatc aggatctttc aatccgcttc
    13981 gtgcacggcg gatcgcacgt gaaactgctt tgacagcctc atcttgacca acgacacgtt
    14041 catgcagaag tccttccagt tctaacaagc gttcgctttc ttttttctcc aactgctgta
    14101 gagggacacc agtccactcc gatacaactg tagcaacatc ttcttctgtt acacgatcag
    14161 aataaccttg ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg
    14221 ctttttcttg tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt
    14281 ccttttcttg tactagttca tcgatttcta gtttgatcac agttgatttt gttaagtgat
    14341 cggtttgatc caaacggact ttcgctgctg attcatcgat caaatcaatt gctttatcgg
    14401 gtagttgacg agaagtgata taacgaacag aaagattgac tgctgctcgt acagcttcat
    14461 ctgtgatctc cactccatgg tgctcttcat atcttgtacg taatccttga agaatcactt
    14521 ctgcttcttc aggagttggc tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag
    14581 aatctttttc gatatatttt tgatattcat ctagggtcgt tgctccgatc gtttgtaatt
    14641 ctcctcgagc taacgccggt ttcaagatat tcgaagcatc aattgctcct tctgctccac
    14701 ctgcaccaat taaggtatgc aattcatcaa tgaataagat tacttgacca tcattataga
    14761 tttcgtcgat gacctttttc attcgatctt caaattcacc gcgatatttc gtaccagcaa
    14821 ccaaagatcc catatcaagc atcatcaggc gcttttcttg catgtcttct ggcacttcac
    14881 ctaaaatgat tttttgagct aacccttcag caatggctgt tttgcctaca ccaggttcac
    14941 ccaccagtac agggttattc tttgtccgtc ggctaagaat ctggataagc cgtttcactt
    15001 ctctcgaacg accaacaact gggtccagct tgttttcttt tgctaatttc gtcaaatcac
    15061 gtgctaatga atctagcgta ggtgttcctt gcggtgtttg ccgtttatct tgtacattta
    15121 catttctacg acgattcatt ccttttccag cagaatcacc agctagcccc attttttgtt
    15181 tcagaacttg acgggctttt gaaaggctga tacctaagtt gatcatgatt cgagtagcca
    15241 tgatactttc atcttttaat aagctcatca aaatatgttc tgttccaact ttttgagaac
    15301 cagttttctt cacttcgctg ctagctaaag caaaaacttg tttcattctt ggtgaatatg
    15361 gaagaaagac tcctttaggg taagaacgga taccaccata accaatcaga tgttcaattt
    15421 cttcataaat ttccgtttca ttcaaatcca tttcacgtaa tacttttcca gcaattccat
    15481 tttgctcgac cactaaggct agtaaaacat gttctgaact aactgattga tgtttcaacc
    15541 gttttgcttc ttcttgtgca atttctaata ctgcttgggc tctttcagta aaaagttctt
    15601 ccatctaatc tcacttccca tcttcataac ttaaacgttc cagaaatgct cggagaatcc
    15661 gagcacgtgt atgatcttca tattcattga aattcaatgt gttttttgca attgcactca
    15721 acatcaagtt tccttctttt tttgtaatta tttggtcttc ataaagtttt tgaataatag
    15781 caaaagcatt tttctcactg attgtatcat caaataactg atttatctgc tcaagtaatt
    15841 gttttttatc tgaaatcctt acttttgcaa ttcgtatata accaccacca ccacgtttgc
    15901 tttctactag ataaccacgt tggatggtaa aacgggtatt aatcacatag ttgatttgtg
    15961 aaggaacaca gttaaagagg tctgccattt caatccttcg aatctcgatc atttcactat
    16021 tttccagaat tttctttata tatgattcaa ttaaatccga ggtattctga tgtgccatct
    16081 tattcattcc ctttgactaa tattgacctt aattataacg aatttgataa tagaaagaaa
    16141 gaagaacgct ttttgttaaa taaatccttt agaatcaaca aaaaaaaata cggaatatac
    16201 tattccgtat c
//
LOCUS       DAZUMI010000064        15878 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000064 DAZUMI010000000
VERSION     DAZUMI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.064"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..858
                     /locus_tag="ACG81C_001771"
     CDS             106..858
                     /locus_tag="ACG81C_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5064925.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            855..1769
                     /gene="pfkB"
                     /locus_tag="ACG81C_001772"
     CDS             855..1769
                     /gene="pfkB"
                     /locus_tag="ACG81C_001772"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5064926.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1787..3718
                     /locus_tag="ACG81C_001773"
     CDS             1787..3718
                     /locus_tag="ACG81C_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5064927.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3829..5388)
                     /locus_tag="ACG81C_001774"
     CDS             complement(3829..5388)
                     /locus_tag="ACG81C_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5064928.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5543..7777
                     /gene="pcrA"
                     /locus_tag="ACG81C_001775"
     CDS             5543..7777
                     /gene="pcrA"
                     /locus_tag="ACG81C_001775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5064929.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7794..9830
                     /gene="ligA"
                     /locus_tag="ACG81C_001776"
     CDS             7794..9830
                     /gene="ligA"
                     /locus_tag="ACG81C_001776"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5064930.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9936..10241
                     /gene="gatC"
                     /locus_tag="ACG81C_001777"
     CDS             9936..10241
                     /gene="gatC"
                     /locus_tag="ACG81C_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5064931.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10241..11713
                     /gene="gatA"
                     /locus_tag="ACG81C_001778"
     CDS             10241..11713
                     /gene="gatA"
                     /locus_tag="ACG81C_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5064932.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11710..13140
                     /gene="gatB"
                     /locus_tag="ACG81C_001779"
     CDS             11710..13140
                     /gene="gatB"
                     /locus_tag="ACG81C_001779"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5064933.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13150..14205
                     /locus_tag="ACG81C_001780"
     CDS             13150..14205
                     /locus_tag="ACG81C_001780"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5064934.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14316..15689
                     /gene="rlmD"
                     /locus_tag="ACG81C_001781"
     CDS             14316..15689
                     /gene="rlmD"
                     /locus_tag="ACG81C_001781"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5064935.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15692..15820
                     /locus_tag="ACG81C_001782"
     CDS             15692..15820
                     /locus_tag="ACG81C_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5064936.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5397 a         2902 c         3480 g         4099 t
ORIGIN      
        1 ttattttttt ttgactgatt tataattttt atgaacgttt ttgactgatt tatattgcga
       61 gtagaaaaaa aatatgctat tatttgtatt gaggtgatcg tgaaaatgct tacagaggaa
      121 cgtagacaaa aaatcctaga cttgcttgaa cagcagaaaa tcgtgaaatc acaagatctt
      181 gtaaaccttt ttaatgcttc tgaatcaacg ataagacgtg atttacaaga gctggagaat
      241 agtgggtttc tagaaagagt tcatggcgga gctaagaaag agcaattatt gaattttgag
      301 cagaatatga gtgaaaaatc actcaaaaac gttcacgaaa aacagcaaat cgccaaatcg
      361 gcagctgaaa ccattaatga cgaagatgtc atttatttag acgctggctc tactacgtta
      421 gaaatgattc cttttctgca aggaaaacag atcacagtag tgacaaattc cgttcaacat
      481 gcggcaaatt tagttgattt ggagcttgct accattattt taggtggaac gataaagctg
      541 gcaactaatg ctgttttagg atctaatgcg atagaacaac taaaaaattt tcattttaat
      601 aaagcattta tgggaatgaa cggtgcccat cttgaacaag gattcacgac tccggaccca
      661 gaagaggcag ctgttaaacg tttagccatg aaaaattccg aagagacatt tgtattgatt
      721 gatcatacca aattcaacca gttgtccttc acctctgttg cacctttgga agcagccacg
      781 atcataaccg agcgttgtcc attagaattc gttaatgaat tccgtgaaaa gacaacaatt
      841 aaggaggcaa tacaatgatt tacacagtaa cacttaatcc atcaattgat tatatcgtac
      901 gcgtagatgg gttaaaacta ggcgatttga accgaatgaa ggaagacttc aagttacctg
      961 gaggaaaagg gatcaatgtt tcccgtattt tgaaacggat cgattctcaa tctacagcac
     1021 tagggttttt aggagggttc actggcgaat ttatttcgaa gtggcttgaa aaagaacaaa
     1081 tccagtcttt gttcacaacc gtaaaagaag atacaagaat caatatcaaa ctaaaatcag
     1141 atactgaaac tgaaattaac ggtttgggcc catctatttc agatgaagaa atcgatgagt
     1201 tgaagcaagc attaggaaca gtaggacaag gagatatcgt cgtattgtca ggaagcgcgc
     1261 cggctagcct gcgaaaagga ttttatcaag aactgattga tatcattcga gaaaaaggtg
     1321 cagatttcgt gattgataca acaggtgatg acctaaaaaa tgcacttgtt aagaaaccat
     1381 tgttgatcaa accaaacaat cacgaattag ctgagcttta caatacaacc ttccaatctg
     1441 tagaagaaat cattccgtat ggtaaaaaat tgctagctga aggggcacag aatgtgctga
     1501 tctcaatggc tggagacggt gctttgctct tcacggaaca aggaacttat aaaacgaatg
     1561 tattagttcg cccattgaaa aattcagtag gtgcagggga ttcaatgatt gccggcttta
     1621 ttggggctta cagccgtacg aaagatccaa tcgaagcctt taaatgggga gttgcttgcg
     1681 gaagcgcaac agctttttct gatgacttgg cagctcgcga attgattgat gagttgctgc
     1741 cagaagttga aatcacaaaa attgattagt ttcaaggagg ataatcatga atatcaagga
     1801 tttaatggtc aaagaagcga tgatcatgga tttgcaagcg actgataaaa aaggcgcaat
     1861 cgatgaaatg gtccaaaaaa tgtttgatgc cggccggatt tccgatatcg aaacatataa
     1921 agaaggaatt ctagcccgcg aagcacaaac ttctacaggt cttggtgatg gaatcgccat
     1981 gcctcacgcg aaaaacagtg cagttaaaga agcaacagtt ttattcgcaa aaagccaaaa
     2041 aggagtagat tatgaagcct tagacggaca gccaacttat ctgtttttca tgatcgcagc
     2101 tccagaaggc gcaaacgaca cacatttgca agcattagca gcactatctc gattgctgat
     2161 tgatgcagat ttcgtaggaa aattaaaaga agcaaataca ccagaagaag tccaagactt
     2221 gttccaaaca gctgaagagc aaaaagaagc agaagaaaaa gcagaacaag aaacacaagc
     2281 tgcagaagct tctgctcatc cagaaagaaa attcgttgtt gccgttacag cttgtccaac
     2341 agggatcgct cacacttaca tggcagaaga tgctctgaag aaaaaagcga aagaaatggg
     2401 tgtggagatc aaagtcgaaa cgaacggttc agaaggaatc aaaaaccgat tgactgctga
     2461 agacatcgcc cgtgcagaag gtgtaatcgt tgcagcagac aaaaaagtag agatgaatcg
     2521 ctttgacgga aaagaactga tcaatcgtcc tgtcagtgat ggtatccgta aaccagaaga
     2581 gctgatcaat ctagccctta gtggtactgc accagtattc catggtgatg gaaaagaaag
     2641 cagttcagaa gaaggaaatg cggatggtac tatcggacaa agaatttaca aagatttgat
     2701 gaacggcgta tcacacatgt tacctttcgt tattggtgga ggaatcgcta ttgcgctttc
     2761 tttcatgatc gaccaattta ttggcgtgcc acatgatcaa ttatcaaatc tagggaatta
     2821 caatcaagct gctagctggt tcaaccaaat cggacaagct gcatttggtt tcatgctgcc
     2881 ggtattagca ggatttattg cttcaagtat cggtgaccgc cctggtttga tcgttggatt
     2941 tgctgctggt gcattagcta atacaggcgg tgcaggattc ttaggtgcac tgattggtgg
     3001 attcttagct ggttatgtca tcgtattttt acgtaaatta ttcaagaact taccaaaatc
     3061 attggatgga attaaaacga tcctctttta tccagtgttt ggcttactga ttactggttt
     3121 cttgatgttg ctggtcaatg tccctatgaa agcaatcaat gatgcattga acggtttctt
     3181 gacaagtatg agtggttcga atgctgcttt acttggtgca ttattagctg gaatgatggc
     3241 ggcagattta ggcggtccaa tcaacaaagc cgcttatgtg tttggtacag caactttagc
     3301 tacgactgtt gcaactggcg gaagtgtcgt gatggcttct gttatggctg gaggaatggt
     3361 acctccattg gcaattttcg ttgctacacg tttgtttaag aataaattca gcaaaacaga
     3421 tcaagatgct ggtttgacga acatcgtgat gggactatcg tttgtcacgg aaggatcgat
     3481 tccatttgca gcagctgacc cgattcgtgc cattccaagt tttattattg gttctgcttt
     3541 aactggcgga ttagtcggag ctttcgggat caaattactg gctcctcatg gcgggatttt
     3601 cgttgtattc ttactgagtc acccggtcat gtatttggtg tttattgcta tcggagcagt
     3661 cgtatctggt gtgatttatg gtttattgag aaaagctcct gcagcagcag aagcatagaa
     3721 ataaaaaaga agaggctggt ttttcccagt ctcttctttt tttatagaat catatagaat
     3781 caagtagtgt agatgaatag ttgttgtaaa atagtttttc catgttcgtt aaaatgattg
     3841 cttcaaattt gtgatagaag atttagcctc gatttcctgc ttgattcttg gcaagtttgc
     3901 ttcatcagtc aagataacca aaatatctcc tacattcatg atcgtatcac cgtgagtcaa
     3961 gatttcctga tttcctctgc gtatagagat tagcagcata tttttaggcc aattgaagtc
     4021 tcgtaccatt aatccgtcta aagaactctc tgccgttact ggaaaatcaa aagttgtcct
     4081 tgcacctttt agtgaggcgg tctgagtatt tccaagtaga cgttccaata atgattcgta
     4141 aattggatta ccgccgagaa ggtcattgac gatataagca acaagagcag ccaatcctaa
     4201 aggcatcaga tgattaagag agccaaccat ttctgtcact aaaataattg ctgtcagcgg
     4261 tgcttttcca atcgctgtga aataaccagc catggcaata acgacaaaat ccttgatgta
     4321 gattggatcc atcccgaaat caaccataat gtttccataa aagctaccga ttaaagcccc
     4381 caatgtcaat atcggaagga aaatcccccc tggaagacca ccaccgtaag aaatcatgga
     4441 aaaaacgaat cgaagaacaa acaaaccgat aatcataatg attggcaagg aagcatgtcc
     4501 aaaagctaaa acgatttgat tgcctccgcc tagagtacta ggccataaat aaccaattgg
     4561 caaaatcagc aagaaaggaa cgaacccgtg aaaataagct ggtaaaggca atcggttgta
     4621 aaattttggc aaagccagca agaccttctg ataaataaaa cctaagatgc ctagaagtac
     4681 tcctaagatc actaaggaac cataatattt caacggcaaa gaaggaaggt cacctagata
     4741 taaaacaggt ctcaagccga aaaagtaaag agagacgaag ttagcagtga ttgctgaagc
     4801 aaatgatgtc agccaaacaa gtggagaaaa ggagtgatgg atttcttcta aaacaaagag
     4861 taatcctgcg ataggagcat tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat
     4921 taaaatcttt tcttctgaag atggactccg aaacaattgt ccagctcctt gtccaatgga
     4981 tgcgccaagc tgtatagaag gtccttctct acctagaaaa aggcccgcac caactgaaag
     5041 tacaccgcca atgaactttt tccataaaac agagaaccag ttcattgata attccccgcg
     5101 aagctgtccc tctacttgtg gaatcccgct tcccttgata tccggttcat ttttgaccag
     5161 ataacccacg aaaaatgctg caaggatgca gcaaatagct aggccaatta agtaaatcgg
     5221 ctgttcccga ctcatttcat agatacttcg aactaaatcg cttagctttt cgatagataa
     5281 tcgaaacagg ctaacgacga ttcctgtcag cagtccaaca aatattcctt ttagaataaa
     5341 atatatctga gtcttatcta atttcttgat ttttcctttt ttttccattc aaacgcctcg
     5401 ctttctttta atttagcgcg aaatcaacga attgacaagg aaaaatccat aaaaagaaac
     5461 aaatgatttt gaactagcta gtttaatgaa agccaagaaa atgctataat taacaagatt
     5521 gataaaagaa aggatgttgt taatgtcatc caaagctgaa ttattaaacg ggatgaaccc
     5581 ccgtcagaaa gaagctgttc ttcacactga cggaccatta ctgctaatgg caggtgcagg
     5641 aagtggaaag acgagagtgc tgacacaccg cattgcctat ttgattgagg aaaaagaagt
     5701 caatccttgg aacatcctag cgattacttt taccaataag gctgcaaaag aaatgaaaga
     5761 acgtgtcaat gcgattctcg catctggagg cgaagatgtc tgggtatcga cttttcactc
     5821 gatgtgtgtg cgtattttaa gaagagatgt tgattttatc gggtataacc ggaactttac
     5881 aatcatcgat tcttctgaac agctgacctt gatgaaacgg atactaaaag aactgaacat
     5941 tgatccgaaa aaatatgatc ctcgaagtat attaggaaca atctctcaag caaaaaacag
     6001 cctgcagacc cctcaggatt ttgcaaaaat gcaaggcagt tattacgaag agattgcggc
     6061 aaaatgttat gctgcttacc aaaaagagct tcaatataat caatgtatgg actttgacga
     6121 cctgatcatg aatacgattc gattatttga agaacatcct gattcactca catattatca
     6181 aaacaaattc cattatattc atgtagatga gtaccaagat acaaatcacg cacagtatac
     6241 gctagtcaat ttattggcag gtagatttag gaacctgtgc gtcgtgggag atgcagatca
     6301 aagtatttac ggctggcgcg gtgcagatat gcagaacatc ttggattttg aaaaagatta
     6361 tccagatgct gcagtaattt tgctagaaca aaactatcgc tccacgaaaa acatccttag
     6421 cgcagcaaac caagtcattg aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga
     6481 aaacaaagaa ggaaacaaga tcacttacta ccgtgcagat aatgaacgag acgaaacccg
     6541 ttttatcgta gatcggatgc aagaagagat tcgaagcaat catcggaatt acggcgactt
     6601 tgccatcttg taccggacga acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc
     6661 aaatattcct tataaaatgg ttggagggca caagttctac gatcgtaaag aaatcaaaga
     6721 tattttagcg tatttgaatg tgctggcaaa tcctcaagat tcaatcagtt ttgagcgtat
     6781 cgttaattcg cccaaacgag gcatcggacc aggatctatc gaaaaactac gcagttttgc
     6841 ttctcttcat gaatggccac tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg
     6901 tggaaaagca ggacagcaat taggtgcatt tggagagatg atccaagaag tcactcagat
     6961 gatcccatac ttgacagtga cagaattgac gaaagaggtt ttggatcgta gcggatatct
     7021 agaagactta aaaatccaaa atacattaga agcacaagca cgcatcgaaa acttggagga
     7081 atttttaacg gtcacacaag aattcgacaa acaattcgaa cagcaaaatg aagaagacgc
     7141 tgatgcacca gaagaaaaac taacggtctt cttaaatgat ctggcacttg tttccgatat
     7201 tgacaattta gaagaagatg cttcccaagt gacattgatg actttgcatg cagccaaagg
     7261 attagaattc ccagttgtat tcctgattgg tctagaagag ggggtttttc ctttatctcg
     7321 agcattgatg gaagaaagcg aactggaaga agaacgccgt ctcgcatatg tggggatcac
     7381 gcgagcagaa gaagcgttgt atttaacaaa tgctttctcc agaacattat atggacggac
     7441 gcaatataac cgaccttctc gttttgtaga agaaatcgat caggagttac tggaaatcga
     7501 aggcatgcgt cctacgccta aaaaaacacc tgtattcgct aaaaaaacag catatagcta
     7561 taaacagcca gagacagcgg ttgttccctc taaaagtgct actggagggg aaaataacag
     7621 ctggaaacct ggagataaag tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt
     7681 aagcgggacg tcgaaggatc tagaactaga tgtggctttc ccaagtcaag gagtaaaacg
     7741 tcttttggct gcctttgcgc caattgaaaa agcttagtag tggagggtaa atgatggaac
     7801 atgaaatgac gattgacgaa gctgctaaac gagcagaaga acttcgtaca agactgaacc
     7861 aatggtctcg cgaatactac gtagaagata aaccaacagt ggaagactat gtttatgata
     7921 aagaatacgc agaattagta gcaattgagg aacaatatcc tgatttgatc acgtctgatt
     7981 ctcccactca acgagtaggc ggaaaagtat tagaaggttt tgaaaaagtc actcatgata
     8041 ttccgcttta cagtttgaac gacgttttca gcaaagaaga gctgatagct tttgatcaac
     8101 gtgtacaaaa agcagtggga cgtgtagttg actattgctg tgaactaaaa atagatggac
     8161 tttccgtttc tttgcgttat gaagatggaa atttcgtgag aggtgcgact cgcggggatg
     8221 gaactgtagg agaaaacatc actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat
     8281 taaaagaacc aatgaacatc gaagtaagag gcgaatgttt catgccaaaa cggtcatttg
     8341 tccaattgaa ccaagacaga gaagcagagg gaaaagatat ctttgcgaat cctcggaacg
     8401 ccgcagctgg ttctttgcgt cagttagatt ctaaaatcac agcgaaaaga aatctggaca
     8461 cttttttata cacagtagct gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc
     8521 tagaagagct agaaaaaatt ggattccaca cgaatcggga aaaacgtcta tgtcattcga
     8581 ttgatgaagt gtggtcatac attgaagaat atcatgacaa acgtgtggat ttgccctatg
     8641 aaatcgatgg aatcgtcatc aaagtgaatg aattctcgct acaagatcaa cttggtttta
     8701 ctgtcaaagc accgagatgg gcagcagctt acaagtttcc gccagaagaa gttgaaacgc
     8761 taatcgaaaa catcgaatgg acagtcggtc gaacaggggt agtaacgcca acagctatca
     8821 tgacaccagt ccgagtagca ggaacgacag taagccgtgc cagcctgcat aacggtgatt
     8881 atattaagtt gaaggatatc cgcttgaaag atacagttct gatttataaa gcgggagaca
     8941 tcatcccaga agtttcgcaa gtcgtgttag ataaacgacc taaagacagc gaagaatacc
     9001 aattgcctac acattgtcca gtttgtggaa gtgaacttgt ccatttggat gaagaagtag
     9061 ccttacggtg catcaatcct aaatgccctg ctcaaatgaa agaaggtttg aaccactttg
     9121 tttcaagaaa tgcgatgaat atcgatgggt tggggccgcg cgtactagaa cagatgtatg
     9181 ataaaaaact agtagcagat gttgctgatc tttacaaatt gacagaagaa gagttattga
     9241 cgttggacaa aataaaagaa aaatcagcaa ataatatttt gacagcaatc gacaacagca
     9301 aagataattc ggtcgagcgg ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag
     9361 cagcaaaaat tttagcagaa catttcggtg atctagaaac attgagtaaa agtgactatg
     9421 agtcgattat tgcgcttgat acgatcggtg atatcattgc cgacagtgta gtcacttatt
     9481 tcagtaacga agaagtccat gaattgatga atgaattaaa acaagcaggc gtcaatttcg
     9541 aatacaaagg gcttcgaaat gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa
     9601 cagtagtcct tactggtaaa ctaacacgat ttactcgtga agaagcaaaa gagacgatcg
     9661 aaaatcttgg cgggaaagtg actggtagcg tatcgaaaaa aacggatatc gtggtagcag
     9721 gagaagatgc tggcagcaag ctgaccaaag cacaagaatt aggaatcgaa gtttggacag
     9781 aagatcaaat ggcagatgca ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt
     9841 ttttcagaca aatttcaata aacaatttca gacacggcac tcttgtgtta aaatagagag
     9901 caaagttttt aactcgttta gaaagaaggg gtagtatggc gattagtgaa gaacaagtga
     9961 aacacgttgc caaattagca aaacttgcgt tcgcagatga agaacttgca agttttaccg
    10021 accaattagg taaaatcatc gatatggttg aaatgctgga agaagtagat acagaaggtg
    10081 ttccatttac gtcgaatgtg gttgaaacag taaacgtgat gcgacaagat cgggcgcaag
    10141 ctggatggaa ccgcgatgaa ttattgaaaa atgtgccgga aacagaagac ggctttatca
    10201 aagtacccgc aatcatcgat aatggggagg ctggcgcata atgacagaat tacacaatcg
    10261 ttctctagaa gaactgcatc atatgcttgt atcgaaagaa atcactgtac aagatttgac
    10321 aaaagcaaca tttgaacgaa tcaaagaaac agagcctgag gtcgaagcat tcattacatt
    10381 gaacgaagaa aaagcattgg aacaggcaaa agctttagat gaaaaaggga ttgatgaaag
    10441 caacgtcctt gctggtattc caattggtat caaggataat atcgtaacaa aagatatctt
    10501 gacaaccgct gcatctaaga tcctttacaa ttttgaacca atttatgatg caacggtcat
    10561 ggataaaatc tatcaagcag atatgatccc tgttggtaag ctaaacatgg atgaatttgc
    10621 tatgggtgga agtacagaaa cttcttattt caagaaaaca aaaaatgctt gggacccaac
    10681 aaaagtacct ggtggctctt ctggtggttc tgcagcagca gttgcagctg gacaaattcc
    10741 agtttcttta ggtagtgata ctgggggaag tatccgccaa ccagcagctt ttaacggcat
    10801 cgtagggatg aaacctactt atggccgtgt ctcacgtttt ggtttgattg cctttgcttc
    10861 atctcttgac caaatcggac caatgacccg tacggtgaaa gataatgcat tagcattgac
    10921 agctatcagc ggttatgacg aaaaagacgg tacttcttca ggcgtttcag ttcctgattt
    10981 tgcagaggga ctgacaggcg acatcaaagg aatgaagatt gctttaccta aagaatactt
    11041 aggcgaaggt gtggcccctg gcgtaaaaga agctgtattg aaagcagcag aaacattccg
    11101 ttcattaggt gctgttgtgg aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt
    11161 ttattatatc attgcctcat cagaagcaag ttccaacttg caacgattcg atggtatccg
    11221 ctacggttat cgctcagaaa atgtgaaatc attagatgat gtatatgtca actcacgttc
    11281 agaaggattc ggtgaagaag taaaacgccg tatcatgtta ggtacattct ccttaagtgc
    11341 tggttattat gacgcttact tcaaaaaagc gggacaagtc cgtacattga tcaaacgaga
    11401 ttttgaaaaa gtctttgcag attatgactt gatcatcggg ccatcttcac caacagttgc
    11461 attcggaatc ggtgagaaca ttaacgatcc aatcacaatg tacatgagtg acatcttaac
    11521 gatccctgtc aacttggctg gacttccagg tatgtctgta ccggcaggtt tatcagaagg
    11581 attaccagtt ggattgcaga tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc
    11641 ggcatatgcg tttgaacaag caacagaatt ccataaacaa caaccagcta ttttaggagg
    11701 gaaagacgaa tgaattttga aacagtcatt ggactagaag tccacgtcga gttaaaaacc
    11761 aactcaaaaa ttttctctcc agcgccagct cattttggtg cagaaccaaa cagcaataca
    11821 aacgtgatcg actggggtta tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa
    11881 ttcgggatga aagcagcttt ggcattaaac tgtgaaatct ctaaagatac tcattttgac
    11941 cggaaaaatt acttttaccc agataatcca aaagcatatc aaatttccca atttgatcaa
    12001 ccaatcggtc atgacggatg gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg
    12061 atcgaacgtg tccatttaga agaagatgct ggaaaaaaca tgcatggtat tggcggttat
    12121 tcttacgttg atttgaaccg acaaggcacg cctttgatcg aaatcgtttc tgaagctgat
    12181 atgcgttcac cagaagaagc atacgcttat ctagaagcat tgcgatcaat tatccaattt
    12241 acagaagtca gcgatgtgaa aatggaagaa ggctctatgc gctgtgatgc caatatttct
    12301 cttcgccctt atggacaaga agagttcggt acgaaagcag aattgaaaaa cttgaactct
    12361 ttatctttcg tgaaaaaagg tttagctttt gaagaaaaac gtcaagcaaa agttcttctt
    12421 tcaggaggag aaatccaaca agaaacacgc cgttttgacg aaacaacgaa taaaacagtt
    12481 ttgatgcgtg tcaaagaagg gtctagtgat tatcgttact tcccagagcc tgatatcccg
    12541 aaattcgtga tcgatgacga atggattcaa aaagtaaaag ctagtttgcc tgagatgcca
    12601 gcttctcgtc gcgaacgcta tatccgtgag tttggacttc cagagtatga cgcaatggta
    12661 ttgacactta caaaagaaat gtctgatttt tttgaagctg ttttggaaaa tggtgcagat
    12721 gccaagcaag catctaactg gttgatgggt gaagtttctg cttacttgaa cagtgagaaa
    12781 ctggaattag ctgagacaaa attgacacct gagaacttag caggaatgat tcgtttgatc
    12841 gccgatggaa cgatcagctc aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat
    12901 ggcggagaag cccaagaagt agttgaaaaa aatggttggg tgcaattatc tgatccgaac
    12961 aaactactgc caatcattaa tgaaattttg gataacaacc aacaatctgt cgatgatttc
    13021 aaaaatggaa aagatcgtgc ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa
    13081 ggacaagcaa atcctggtgt cgtgaataag ctacttaaag atgaattagc gaaacgatag
    13141 aggctgaaaa tgaaaaaagc acgcgtaatt tataacccca cctcgggaaa agaattattg
    13201 aaaaaaaatt tggcggatat cctacaggca ttagaagaag caggatttga agccagcgcc
    13261 tatgcaacta caccagaacc agattcagcc aagaatgaag catgtagagt cgcagaacta
    13321 ggttttgatc tagtagtggc agctggtggc gatggcacaa tcaacgaagt ggttaatgga
    13381 atcgctccgc ttgaacaccg tcctaaaatg gccatcatcc cagctggtac aacgaacgac
    13441 tatgctcgag cattaaaaat tcctagagat aatattaaag cagcagcaga agtgataaaa
    13501 aaacaccaaa ccgtaaaaat ggacatcggc ctttctgata acagttactt catcaatatc
    13561 gctgcaggtg gctatttgac agaattgact tatgaagttc catcagaact aaagagtatt
    13621 tttggttatc ttgcttatct tgcgaaagga gcagagatgc tccctcgagt aaagccaatc
    13681 aaaatgcgct tgaagtatga tgaaggggaa tatatcggaa atgcttcgat gtttttccta
    13741 gggctgacga actctgttgg aggattcgaa cagatcgcgc cagatgccaa attggatgat
    13801 gggaagtttt ctttaatcat tgtgaagaca gcaaatatct ttgagatctt gcatattgca
    13861 gcactcatgc tgaatggcgg tcgacatgtc gatgatccaa gaataatcta taccaaaaca
    13921 agcagtttgt tcgttgaaac agaagatgat atcaatcgac cagtaatgat caacttagat
    13981 ggtgaatacg gcggggaagc accgatgcat ttccaaaatc ttcatcaaca cattgagttc
    14041 tttgccaata ccgatcagat tcctgatgaa gcaatcacag gtacagatga agaagaatta
    14101 gaggttgtca gcaaagaatt cgtaaaagaa gtcgaacgtt taacggatga agatatcgac
    14161 ggtgacggaa aaatagcaga cgaaacacct aatcaaaaga attaatcaaa agccggtgct
    14221 gaacgtagta aggtcagtgc cggttttagt tgtgtaatta gctgatttat agtagagttc
    14281 agtaggattg aaaaataggc ttaaaggagc aaaatatgac agaacagcaa atagtaaaga
    14341 aaaacgaacg atatacagtc gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa
    14401 aaatcgatgg ttatccatta tttatcgaaa atgcactccc tggagaagaa gtagaaatac
    14461 tagtagtaaa agcaggcaat aagtttggat atggaaaagt cgaaacattt attacacaat
    14521 cacctgatcg tcagcctgta gaaaacaatg cattacttcg cataggtatc gcacctttag
    14581 cccatttgaa gtatgaacaa cagcttgtct tcaaacaaaa acaagtagag catgtaatgg
    14641 cgaagattgc taagatgcca gaagttcctg tacttccaac ggtcggaatg gcagaaccat
    14701 tcggctaccg taataaagca cagatccctg ttcgccgtat cgatggacag ttagccaccg
    14761 gtttttacaa aaagaatagc catgatctag tccctatcga agatttttat atccaagatc
    14821 ctgtcatcga tcaagcaatc aaagtagtca gagatatctt gcaacggttc caagtacgag
    14881 gatataatga agaaaaaaat gaaggacagc ttcgtcacat cattatccgt cgtggtcatt
    14941 attcccatga aatgatggtc gttttagtta ctcgtaaaga aaaattcttt aaatccaagg
    15001 aaattgctga agctattcat gaagaattgc cagaagtggt ttcggtcatc caaaatatca
    15061 atgaagaaaa aacaaatgtg attttaggag acaaagaaaa agttttatat ggcagagact
    15121 atatcgaaga tcagctatta ggaaaaacct atcgaatctc agcaaagtca ttttatcaag
    15181 taaatactca acaagcagag tatctgtacc aaacagccat cgagtttgcc gagttagata
    15241 aaaaagacat tgtgatcgac gcttattcag gaataggtac gattggcttg tctttagcag
    15301 acagagtgga taaggtgtat gggatggaag ttattccaga agcaattgaa gatgcacagt
    15361 tcaatgcttt aaataataaa atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag
    15421 ttatgaaaaa atggcaagaa aaagggatca aaccatctgt gatcgtagtt gaccctccac
    15481 gaaaaggatt agatcatcat tttattgaat cagcaatcga aatgtcacca gaacgaatcg
    15541 tctatatctc atgtaatccg gcaacttttg caagagatgc taagttattt gcagaagcag
    15601 gttatgccgt gaagaaagtt cagcctgtag atcttttccc gcaaacccat catatcgaat
    15661 tagtcgcttc attcgttaaa ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt
    15721 gatcaaaaag aaacaaggtg gaggccgatc caaacaaatg gaaacaccta agaatgatcg
    15781 taaaaacatg agaaaaggcc ctaaaatctt taataaatag acgtgctaaa gaaagactaa
    15841 gaagtgttaa cttttgttat tgtttcttag tcttttta
//
LOCUS       DAZUMI010000065        15431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000065 DAZUMI010000000
VERSION     DAZUMI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.065"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..2214
                     /locus_tag="ACG81C_001783"
                     /pseudo
     CDS             155..2214
                     /locus_tag="ACG81C_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2297..2866
                     /locus_tag="ACG81C_001784"
     CDS             2297..2866
                     /locus_tag="ACG81C_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5064937.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3041..3847)
                     /locus_tag="ACG81C_001785"
     CDS             complement(3041..3847)
                     /locus_tag="ACG81C_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064938.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3985..5499
                     /locus_tag="ACG81C_001786"
     CDS             3985..5499
                     /locus_tag="ACG81C_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5064939.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5902..6885)
                     /locus_tag="ACG81C_001787"
     CDS             complement(5902..6885)
                     /locus_tag="ACG81C_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5064940.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6890..7537)
                     /locus_tag="ACG81C_001788"
     CDS             complement(6890..7537)
                     /locus_tag="ACG81C_001788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5064941.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7622..8254)
                     /locus_tag="ACG81C_001789"
     CDS             complement(7622..8254)
                     /locus_tag="ACG81C_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5064942.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8270..8821)
                     /gene="coaC"
                     /locus_tag="ACG81C_001790"
     CDS             complement(8270..8821)
                     /gene="coaC"
                     /locus_tag="ACG81C_001790"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5064943.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8818..9555)
                     /locus_tag="ACG81C_001791"
     CDS             complement(8818..9555)
                     /locus_tag="ACG81C_001791"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5064944.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9552..10541)
                     /locus_tag="ACG81C_001792"
     CDS             complement(9552..10541)
                     /locus_tag="ACG81C_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5064945.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10799..11341
                     /locus_tag="ACG81C_001793"
     CDS             10799..11341
                     /locus_tag="ACG81C_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5064946.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11358..12443
                     /locus_tag="ACG81C_001794"
     CDS             11358..12443
                     /locus_tag="ACG81C_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5064947.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12455..13261
                     /locus_tag="ACG81C_001795"
     CDS             12455..13261
                     /locus_tag="ACG81C_001795"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064948.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13262..14089
                     /locus_tag="ACG81C_001796"
     CDS             13262..14089
                     /locus_tag="ACG81C_001796"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5064949.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14086..15159
                     /locus_tag="ACG81C_001797"
     CDS             14086..15159
                     /locus_tag="ACG81C_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5064950.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4960 a         2787 c         3140 g         4544 t
ORIGIN      
        1 ataagttatt attccgattt tcttgaaaca ataaaaaatc aaaaaaaata ctctcctaat
       61 agtccatctt cttttgattt tatttatata atgaaatcat cttatgaaaa ttactacttc
      121 atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac gttcaaaaca
      181 actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa aacactacgt
      241 taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata tgcatgtacc
      301 cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg agaccactga
      361 ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc tacttcctgc
      421 attgacttcc tataataaag tcagttatcc tccaacgata aaccactact ggttaattcc
      481 taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta tcttttccga
      541 tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt tgaatcatgc
      601 tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg aaaaagatta
      661 ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt cgatatctga
      721 tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca aagtaatttc
      781 aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt ccttgtccta
      841 ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg ttcgtgctga
      901 tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt acgcccctaa
      961 agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag aagaaatcaa
     1021 aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat taacttatat
     1081 agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag aagcatctgc
     1141 tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag aacttcaagc
     1201 agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg ctgaattttc
     1261 tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag tcacttctat
     1321 ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg agatccttga
     1381 agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa ttacctttac
     1441 ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg ttcatccaaa
     1501 cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag gtaaacccgt
     1561 accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg gttatttgac
     1621 caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt cactccaaga
     1681 actccatgct cagtcactat tacatgtttt accattaaac aaacagatag atcaaaaaga
     1741 acaaaaaaga caagaagaaa gcttgtcaga aaaaaacaac aaatggctca tgcagtttct
     1801 ccaacaggga aagtaaaaca aacaagaagc ctagagttag caagaatatc tagaaaaatc
     1861 tcagaatcta ataaaacgat aaggcaagaa gctcatttgg ccccacaagt ttctggattg
     1921 gaaaatgaaa ttttagagcc taaaataaaa caacaatcag aaaaagaatt tgttgctaaa
     1981 cagtcggaag atcagaagga atcagatgac cagaaggaaa cgaagaaaaa acgcttgtca
     2041 actattgatt ccaccgatga gcatctgcag ccgcaagtac cacttcctag tccgggaaca
     2101 cccgcacctg gtggaggtat tccttcttct gctttaggag atttcatgcg aggtatggca
     2161 ggaacatggg tatacgctaa tcgaaatgaa tttgacgcag atgactggtt ctgatttata
     2221 tctctatctg ttatagtcaa ataagtagct gagtcatatg ttatttggca taatggagac
     2281 gaggagggat agaaaaatgt tagataaaac gttaccttac gcagaaatct ggatgacaag
     2341 accacttcaa caaaatgttc caatcacgcc tttagcggaa ggttttgaat ttcgatttta
     2401 tcaaaaagaa gatgaatctg cttgggcgga gattgagact tccgttgggg aatttcaaac
     2461 gacagatgaa gcacttgagt attttcaaca aaactttgct ccttatcctg aagaacttga
     2521 aagaaggatg ttttttgtag agacaaaaaa aggagaaaaa gtagcaacct gtaccgcttg
     2581 gcgaaaaaaa tcaagagagc aagtgtatcc ggtttttcat tggttagcag tgaaaccaga
     2641 ttaccaagga aaagggctcg caaaagcatt gactgccaga gtcttgcaag aatttcctac
     2701 cctccatacg gacggaccaa tctatcttca cacacagaca tggagccatc aggcgattgc
     2761 tctttataaa aaaatgggtt ttacttttat tgctgaaaat ctagacggca cacctaatcc
     2821 agattatgaa aaagttatgc aggttttaag taaattagat aaataacgaa atggtgaaat
     2881 aagtcctcag tattgggata aaaaacgaaa tagtcattcc tcatatctta cttttttgag
     2941 ttaatactat cggataacta atggcatgcc atacattcaa aaaagaaggg gcagtgacaa
     3001 aagtattgtc actgcccctt ctcttgtttt ttagcatgtt ttatcgtttg atttttttag
     3061 gaataaaatt cttgatgaac gtaaattttt ggtaatcgtt tcgactatag aggtaaccaa
     3121 atacactgaa aacaactgct ccaataaaat taacaaataa atccttcata gtatccatga
     3181 ttcccacgtc taagtaacca ttttggatga ttgttttatc tgttttacca tctgcatttt
     3241 gactttcaat gattgtccga gtgatatctg gaacatgata gacagtgttt ccgtctttac
     3301 ttaactccac actattgatt tgctggatga tcgtatcttt ttgcatatcg aattgcagta
     3361 atttatctgc tgaaaattcc acaaactccc acataacacc aactgtcatc gaaaaacaga
     3421 aggaaacaac tgctagtaat aaaggagtca agttcatggt ttttgtattt ttattgagca
     3481 aataaacaag ggaaaagccg acacccccag ctaaaaatcc gctgaatgca tgtagaaaag
     3541 tatcccacat tgaaaaatag ccataaaaat cacttaactc tcctaaaact gttgaactaa
     3601 aaataaaaga taacaaaatg atttctagca tatttggaat ctgtatcttt aatatctttt
     3661 ccagaaaata agggatttca aataaaatta atgtcagtaa taaaagcatt gtatttcccc
     3721 agtgttggtt ccagacctga tccactagca taataacgac gatgatacgt aataaaatat
     3781 agattccgtt tgtgattcta cctgcagttt ttgtactgtt cgtaaaaaag gttttcaaat
     3841 gattcataaa ataactcctt catgtatatc cttaattata ctaagaagcg tgtgtttacc
     3901 aaagctatat gacttaaaga aaaacagagt tgatattgtg aaaaaaatca cttaatatag
     3961 tataaggaga aaagggggat acaaatgaaa aaattagttg ctggtttatt aacagccgtt
     4021 gcacttcttg ctatgagcgc atgcggcgca gatcagaaaa atacagcgca gacaagttct
     4081 agtacgaagg aatctgccga agtatcatca ggcgcttctg aacaagtata tacagatcca
     4141 aaagaaatga aagaatctta tgatgtcgtg attattggag ctggtggtgc agggatggca
     4201 gcagctatcg aagcaaaaga tgctgggttg aatccagtaa ttttggaaaa gatgcctgtt
     4261 gccggaggaa acacatctaa atcatcaagc ggtatgaatg cttcagaaac aaaatttcaa
     4321 aaagaagaag gaatcaaaga ctcgaatgat gccttctatg aagaaacgct aaaaggtggt
     4381 ggaggaacga atgataaaga aatgcttcga tactttgtcg atcactctgc tgatgcggtc
     4441 gattggctag atcaaaacgg tatcaaactg gacaacttaa cgatcactgg agggatgagt
     4501 gaaaagagga cacatcgtcc gtctgatggc tcggctattg gtggatactt agtggagggg
     4561 ttattgaata acgtccatga gcgggaaatc cctatttttg tcaacgcaga tgtgacagac
     4621 attaagaaaa atggggaaac ggtcaatgaa gtgactgtac atgttcaagg agaagaacca
     4681 aaagaggtca ccggaaaagc aatcgtagtt accacaggcg gttttggtgc cagcaaagaa
     4741 tttattgaaa aatatcgtcc cgatttagct gattatatta cgacaaatca agaaggatca
     4801 actggagatg gtatcaccat gattgaaaaa ttaggtggac aaaccgttga tatggataaa
     4861 atacaaattc atccaactgt gcagcaggat gagggggtct tgattggtga ggttgttcgc
     4921 ggagaaggag caatcttggt cgatcaagag gggaaaagat ttgtcaatga aatggataca
     4981 agggataaag tatctgcagc aattacagca ttgcctgaaa aatcagctta tttgattttt
     5041 gaccaaggcg tgcgtgatcg tgccacagct attgaatttt atgaccaaaa aggatatgtc
     5101 acaactggaa agacaatgga agaattagca gaaaaaatcg atttaccaaa agaaacacta
     5161 gcaaaaacag ttgatggatg gaatacggat gttgcggaga aaaaagatgc tcaattcaac
     5221 cgtgccacag ccatggaaca tgacttatcg tctccgaatt attatgcgat caaaatcgca
     5281 ccaggaatcc attacacgat gggcggtgtg aaaatcaata cgaacactga agtattagat
     5341 aaagaaaacc agccaattag ggggttatat gctgctggtg aagtcacagg tggcttacat
     5401 ggaaacaacc ggatcggtgg aaattcagtt gcggagatca ttatttttgg gcgacaagcg
     5461 ggcatacaag cagctcgatt tgcttcggaa aatgaatgaa atagaacgta aataaaaaga
     5521 ggttgtgaaa tcagcgtttt gagctaatac cgagggtcag ctgattgaac actgtttagt
     5581 gaagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc
     5641 ttcttatgtt tttaacttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt
     5701 tattcggaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     5761 gtataaacgg cggaagcaga agaaattcct tcggaaataa gctgaaattc acaaaaattt
     5821 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
     5881 tcttttttga tcagcatgat tttattcagt tatcttcaaa atgatttttc cgatgttttt
     5941 gtttgcttcc atatattcat gcgcttgttt gactgcgctg aattggaatg tacggtcaat
     6001 gattggccga atcttgccat tttctagaaa aggcccagct ttttgcataa aagtttgtgc
     6061 gagctctgct ttatactgat cacttctagg tgtcaataat gtaccaagca gttggacacg
     6121 tttcccaatc aatacaccaa ggtctatctc aggcacgatg gtcccaccta gcataccaat
     6181 caagacccaa cgtccatcaa cggctataga agctaaattc ttttcccagt aagaagcgcc
     6241 gatgaaatcc aaaataacat cgactccccg accttcagtg atttttcgta cctcttcttg
     6301 aaagtcttgt tttttatagt tgatcaattc atctgcacca agttctttac aaaaggcgag
     6361 tttttcttct gatcctgcag taacgatgat tttcgcgtga gaaagctgtc tagctaattg
     6421 gatcgctgct gttcccacac cacttgctcc tgcatggatc aagacagttt cgccttcttg
     6481 taattttcca agccagtaaa gtgtttgata agcagtcaaa aaaacttctg agattccagc
     6541 agcagaaaca tagtctagcg tatcagggag aaggattgca cgatcggcag gcatcacagc
     6601 atattcggca taaccaccta gattgactaa tccgtatact tttgtcccag gagtaaaagt
     6661 ggagatatca cttttattct caactacctc tccagctact tcgactccca aaatagggta
     6721 gggaggtttc aaagagactg attctctttt cataatatct gttcgattga cagctgctgt
     6781 atggactttg atcagtaatt cgccttgttt tggtttagga acaggcactt caatttgttc
     6841 taatacatct acagaaccag gatgcttgat tgaaatggca cgcatggatc tattcctcct
     6901 ttgcccattc ttggaatgct gtattcaact ctttgctacc ttcagaaagt actagagtcg
     6961 tacgagaaat atccgtttct tccactagag cggcaagtac agcagcaact gtagagcgag
     7021 tgatcgtttt tgatgccttc tcattaggct tagttaattg aatggaagta acttcttcat
     7081 cgttagtcaa tgtgactggt tgaagaatga caaaatctaa gttagttcgt ttcagccatt
     7141 catctgcata atgtttagta atataataat ctgtcatgga ctcaggccat tttgctggtt
     7201 catctgcata aacagcactg accatcagat agcgagagac atgcgcattt tcagctgcga
     7261 tcactgtttt gattgcgcca tccaagtcta cttgcagaag attctttcct tgagagccgg
     7321 cagcaaaaat caatacatcg atgcctttga atgcttcggt cattttttcc acggaccaag
     7381 ttaaatcaaa agagacatac gaaacattct ctttttttac taatgtttgg gaagcaacat
     7441 ctctcacccc tgcaaaaatt tcatgggaac ttggagctaa atcttttatc aggtgtcggc
     7501 caatttgtcc gtttgcacct acaacaaaaa ttttcatttg gatcatcctt tcgacgtcaa
     7561 atctttccat tcaatagtaa caccaaaaga gaaaaaaatc agagatcggc tacgatgact
     7621 atcagttagt aagagaaggg tgcttgaagt atcttaacaa gacattgcat actgctgcag
     7681 tcgcaagacc tgcgacgatt ccttcaggta taccacttgt aaaaataacc gctagaatag
     7741 ctgaataaac agcatttaaa tttgttccga tgactttggc ataatcttgc tggaataaaa
     7801 agtagatcat attcatcact aaaatcgtat tggtcaatga accggaaagt ccagctaaga
     7861 ataaagaaac ggaatctgct ttttgtttca ttagtttgcg cagaccttta taaacgaaat
     7921 aaggaaccac cccaatcaag atgcgaggaa taaacgcgat cagcaaggct ttccagcttc
     7981 catgttctgt tccgatgact gggataaacg gagaaaagac aaaagacaaa ggtgtttgga
     8041 taactgtgct acggatcatg ctgatcagtc caaatgtacc gccaagaaaa gcacctagtc
     8101 ttggtcctaa aacgatcgaa gcaatgatta caggaatatg catggttgtt gcattgattg
     8161 gaccgatagg gataaaacct aaaggcgtaa cagctaataa aatcaaaata gctaaaaaca
     8221 tcgcagttaa tgtaaagttt tttgtatttt tcataagtac tccttttttc taagcatttc
     8281 tttcattaag agtcagcatg acctgttcga tgatcgtctg aatatcagct aaggcacctt
     8341 ttccaaaatc tccacaagct aaaagagatt ctcttggttc aatttcctga tagccgacag
     8401 atctcaatgt ttccagatta cgctgagtga tcgggttttg atacatattc gtgttcatgg
     8461 caggggcaat caattttggt gttcccactg gaagcgccat tgcgactgtt gaaagaagat
     8521 cgtctgcaat gccgtttgct agcttgccga taatgtttgc tgtagcaggc gcgatgagaa
     8581 acaagtcagc ttgttttgct agttcgatat gattgattac ctgcggattt gattcttgca
     8641 tgacgtctgt atggactgcg cgcttcgaca gagattgcaa ggttaatggc gtgataaaat
     8701 gtgtgctgct tttagacata atcacatcta cctgggcacc taatttgacg aattgacttg
     8761 taagatcagc gcttttgtaa gcagaaatac ttccagtaac gcctagtaag atctttttca
     8821 ttttgattcc tccagtctaa ttttctcagt aatcaatgca gcaatctcag atttactttg
     8881 agcttcttcc actgttccat ctttagataa gagatagccg tggtgtgtct cgccatggat
     8941 ttccgtcaaa tcatttgcga aaataaaatc agttttgtta tttatcagac tattttttgc
     9001 gactgccaga agagattcct ttggtacatc tactaatagt ttgaaaccaa tgatttttgc
     9061 ttttggctgc cattcacgta gataagaaat gatttttggt gttttcttca aaacgagcac
     9121 aagatgctcg gtgttagaag agatctttga aacagtggta tctgtctgtt cattttccgc
     9181 aaaccaattg tccagttctt cttgcgttga agaagtgggt aggttttttg ccagctgttc
     9241 ttctgaaaaa ctaaaagcag gagtaaaatc actgactgcc atactgtgga taatcgcatc
     9301 gtagtgttct tttttcgtta gttcttctaa ttgtaaaaat aagtccagtg cagattcgat
     9361 cgtataaatc gttatattgg agtgtctttt cggtttcagt gcttgtttcg tagtcacgta
     9421 atcgattgtg gtatccggat tggcagctaa ataatcagct aatgcttgcc ctaatcgtcc
     9481 tgtcgaatga tttgtaatag cacgtacttg atcgattttt tcagtcgttc ctccagctgt
     9541 gattagaatc ttcatttatg gtcacctaca tttactagtg ttcttccttg gtgtgttcca
     9601 gcaagtagtt tagatgctgt ttcaggcaat tgatccagag taatctcttg gacgaccagt
     9661 tcatctgcaa tctgcaaatc cgccatctgt tgccataaag aaagacgttg atctattggt
     9721 acattgacag aatcaattcc aatcaactgg atgtttcgca gaataaaagg caagacagtc
     9781 gcgtttagtt tgattccccc agcattgcca cataaagcta ctgcaccgtt ataagagatc
     9841 aatggaagaa gactgcttag ctgctcgcca cctactgtgt cgatgacata gtcgatttgc
     9901 tgttttccta aagcttttgt tgtctctggc agaaattcat caggtcgtac gacttgcaaa
     9961 gcacctagac tttttagcca tgtgacagac tcttcttttc tggaaaaggt aatgattgac
    10021 tggaatccta gtttatgtaa cagggctatc gctgtactgc ctacaccgcc agaagcacca
    10081 gtaacgacaa cttttttatc ggaggtcata ccttgacgga ttaaagcgtt cacacacaac
    10141 atagcagtaa accctgctgt accaaggatc atcgcttgac gcagcgtcat attttttggt
    10201 agaggaacta accagtctcc tggtactttt tgatattggc tataaccgcc tggatgggtg
    10261 acgccaagcc caaaaccagt agcaagtaca agatcttctt ttgaaaaacg tggattgtta
    10321 ctttggacga cagttccgga taaatcaatc cctggagtca tcggatatgt tcgtatcacc
    10381 tgaccagact cgctcattgc caatgcatct ttataattga catcagaata ggcaattttt
    10441 accaagactt cattttcagc caactcagta aatggcagat cactgattgc tgtatggaaa
    10501 acaggttcct ttattacctg aaaagatcga aaagcgttca ttcgtgagac actcctttta
    10561 aaattttctt ttatcatacc ataaagccta ctcttttatt cttgttcttc cgtaaattta
    10621 gacaaaaatt aggtgaaaaa taaagaatca aacaactaga atttattcca aaagataaag
    10681 aaatattcag caaaaaaaat tttacaaagg gttgcctttt tcaataaatt tcgtatacta
    10741 atacgagtgg tagtaaacag gaagtttact attagaaaac ttcgattcgg aggaaattat
    10801 ggaaattggt gagaagttaa gaaatttgcg catccaaaag aatttgacac aagaagaact
    10861 aggcgaacgc acagatttgt caaaagggta tatttcccag ttggaacgag atttaagttc
    10921 accatcaatg gaaacattct tttctattct tgaagtctta ggtgtgactc cagaagaatt
    10981 ttttcatcaa gaagcagcga atctgcaagt ggtttattca aaagaagatc atacggttta
    11041 tttcgatgaa gaaaacgggt atgaattgga atggctcgtg gctgattcga atgaaaaaga
    11101 aatggagcct gtcttgctga cttttgaagg tgcaggagaa tataagacgt ttgaaccttc
    11161 attatctgaa acgtttattt ttgtattaga aggggaatta gagttaagct taggagagac
    11221 ggtctatgtg gcaaaagcag gacagtcgat ctattatcaa gcgacaaaac aacatcaatt
    11281 aaggaatcat ctcaaaaaac gaacaaaagt attgattgtt gcaacgcaat catatttgta
    11341 atataggggg taaaaacgtg gggaaaccaa tcatttcatt tgatcaagta gtgaaacgct
    11401 atgatgatga aacagtatta aaaaaagtaa gttttgagat cgaacaagga aaattttata
    11461 cactgcttgg tccttccggc tgtggtaaaa caacgatcct acgtattata gctggattca
    11521 ccgaagctac agaaggggac atctattttg aagggaaacg gttgaatgat ttgccagcta
    11581 ataagcgaca agtaaataca gtcttccaag attatgcact ttttcctcat atgaatgttt
    11641 ttgataacgt tgcttttgga ttgaagatca agaaaatgtc caaatcagat atcgaaaaga
    11701 aggtaaaaga tgctttgcgt atggtccagc ttcctggata cgaaaaacgg gagattagtg
    11761 aaatgtctgg tggtcaaaga cagcgtgtag cgattgcacg agcaattgta aatgaaccga
    11821 aagtattgct attagatgaa ccgttatcgg cgcttgattt gaaactacgt acagatatgc
    11881 agtatgaact gcgggatctg caacaacgat taggaatcac gtttattttt gttactcatg
    11941 accaagaaga agcgttggca atgagtgatg agatttttgt catgaataaa gggaaaatcg
    12001 tccaaagtgg tacacccgta gatatttatg atgagccaat caatcatttc gttgctgatt
    12061 ttgttgggga gagcaatatc gtggacggtg taatgatcga agataatcta gttgagtttg
    12121 ttggtaagca atttgaatgt gtcgatggcg ggatgcgtct aaatgaacca gttgaagttg
    12181 ttttacgccc agaggatttg acgatcacga caccagataa aggaaaacta gtcgtaacgg
    12241 tcgatactca gctgtttcgt ggtgtccatt atgaaatcat ctgttatgat gaacagcaaa
    12301 atgaatggat ggtccattca acgaaaaaag ctaaagaagg aagcaaagta ggattggcct
    12361 ttgagccaga agatatccat gtcatgcgtt tcaacgagtc tgaagaagaa ttcgatgcac
    12421 gtcttgacag ctacgaagaa taggggggac aagaatgaaa acaatgcgca ggatctattc
    12481 cgtcccttat attttgtggc tcggtttatt cgtgatcgcg cctgtattaa tgatcattta
    12541 tcaatctttt tttgatatga atgggcaatt cacgctgaat aattatctat cttattttac
    12601 gtcggggaca tcgctttcta tgacgatcaa ttctgtatgg tatgcatttt tgatcacgtt
    12661 tttcacgctg atcatcagtt acccgactgc ttatttttta acaaaactga agcacaagca
    12721 attatggctg atgctgatta ttttgcctac atgggtgaat ctgctgctta aagcttacgc
    12781 atttattggg attttcagtg tgcacggcag cgtgaatcat ttctttgatt ttatcgggat
    12841 cgggccgaaa cagatattat tcacagactt cagttttctg ttcgtggcaa cgtatatcga
    12901 gattccattt atgattatgc caattttcaa tgcattggaa gagatgaatc cttctttgat
    12961 cagtgctagt agagatttag gcgcgaatag ctttgagacc ttccgacgag tgatttttcc
    13021 gttatcatta aatggtgtga aaagtggtgt ccaagcagta tttattcctt ctctttcttt
    13081 attcatgctg actcgtctga ttggcggaaa tcgggtgatc actttaggaa cggctatcga
    13141 acagcatttc ttggttacac aaaactgggg gatgggttct acaatcggtg tgattttaat
    13201 cattgctatg ttcatcgtga tgctcctgac aggcgagaag aaaaaaggag gtcgctcata
    13261 aatgaaaaaa tttaaatggt cgtatcttta tttagggatc gtctttttat tcttatatgc
    13321 accgatcttc tatctgattt tttattcctt caatgaagga ggaacgatga acagctttac
    13381 aggatttact tgggaaaatt atgaagctgt ttttgaagat acacgtttga tcattatcgt
    13441 attgaatacg ttcatgctgg ctttcttatc tgcattgatc gccacgatca tcgggacatt
    13501 tggggctatg ggaatctatt acacgaaaaa acgacgtgca cggacagcgt tgttgagcat
    13561 gaataacatc ttattagtct ctcctgatgt tattatcggt gctagtttct tgattttctt
    13621 tacactacta ggattcggat taggttttca gagtgtattg ctttcccata ttgctttcag
    13681 cattccaatc gtcgtcttga tggtcttgcc aaaattgcag gaaatgaacg attcaatggt
    13741 cgatgcggct agagaccttg gtgctaataa tttccaagta gtcaaaaata tcattttgcc
    13801 ttatctttct ccaggaatca tagctggata ttttatggca tttacctatt ctcttgatga
    13861 ttttgcggtt acatttttcg tcacaggaaa tggctttacg acgctctcag tagagatcta
    13921 ttctcgtgca agacaaggaa tcagtttaga aatcaatgcg ctaagtgcgt tagttttctt
    13981 gttctccatg atattggtca ttggatacta ctttattagt aaagaaaacc gtccaaaacg
    14041 gctgaagaaa aacagaagag tacgcgaggg ggtggcacgg ctgcgatgaa aaaattacaa
    14101 tctctgtttt taggtattct catcattatt gcggtgctgc tgcttggtgt tcgtcaactt
    14161 gaaaaaacaa gtggcatgag tggagcaaag gtacttacca tctataactg gggagattat
    14221 atcgatccta gtttgatcac gaaatttgaa aaagaatatg gatacaaagt gaattatgaa
    14281 acatttgatt ctaatgaagc gatgttcaca aaaatccagc aaggtgggac aaactacgac
    14341 atcgctatac caagtgaata tatgatccaa aaaatgatca aagaaaaact agtacttcca
    14401 ttagatcatt cgaagatcaa aggattaaat aatattgatc cacgattttt agacttggat
    14461 tttgatccag ataatcagta ttcgattcca tatttttggg gaactttagg gatcgtatat
    14521 aacgataaat tctttgatgc aaatcaaatt aagcattggg atgatttgtg gaagccggaa
    14581 ttaaaagaca gtttgatgct gattgacggt gcacgagaag ttatggggct ttctttgaat
    14641 agtttaggct attctttgaa tagtaagaat atggcacagc tgacagaagc agcaaataaa
    14701 ttgaatcatt taacaccaaa tgtcaaagca atcgtggcag atgagatcaa aatgtatatg
    14761 atcaatgaag aagcctccgt ggcagtgaca ttttctggcg aagctgccga tatgatgtgg
    14821 gaaaatgaac atttacatta tgtgatccca ccagaaggat ccaatctttg gtttgataat
    14881 atcgttatgc caaaaaccgt gaaaaataaa gaaggtgcct atgatttcat caactttatg
    14941 ctagaaccgg aaaatgcggc acaaaatgca gagtatattg gttattctac accaaatgaa
    15001 gaagcaaaag cgttattgcc aaaagacatt tcgtcggacg aacaattcta tccatctgat
    15061 gacacgatct cccatcttga agtctacaaa gatctaggat cgaaatacct aggcatctat
    15121 aatgacctat tcttagaatt caagatgtat aggagataat ggcaaaactc agtaagaatg
    15181 gggacttatc aacgttctaa agagctaaaa atcgtccttt ttctatctga gttttccaat
    15241 cttctaccaa aagcggatga aacgccgctt tttttgtgtt attttatttt ttctaccacg
    15301 tttttctacc agagaaaagt ggacaaaacc attgtaaaat agggcccgag acgggctagt
    15361 aaatctaaga ttgaaaaagt tacagaagga taaagtaatt ggaaactatg caaatatact
    15421 taacccttct g
//
LOCUS       DAZUMI010000066        15312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000066 DAZUMI010000000
VERSION     DAZUMI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.066"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACG81C_001798"
     CDS             complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACG81C_001798"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5064951.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACG81C_001799"
     CDS             complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACG81C_001799"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5064952.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACG81C_001800"
     CDS             complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACG81C_001800"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5064953.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3371..4678)
                     /locus_tag="ACG81C_001801"
     CDS             complement(3371..4678)
                     /locus_tag="ACG81C_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5064954.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4681..5262)
                     /locus_tag="ACG81C_001802"
     CDS             complement(4681..5262)
                     /locus_tag="ACG81C_001802"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5064955.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5378..5475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5834..7126)
                     /locus_tag="ACG81C_001803"
     CDS             complement(5834..7126)
                     /locus_tag="ACG81C_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5064956.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7123..7746)
                     /locus_tag="ACG81C_001804"
     CDS             complement(7123..7746)
                     /locus_tag="ACG81C_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064957.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7749..8585)
                     /locus_tag="ACG81C_001805"
     CDS             complement(7749..8585)
                     /locus_tag="ACG81C_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5064958.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8602..9489)
                     /locus_tag="ACG81C_001806"
     CDS             complement(8602..9489)
                     /locus_tag="ACG81C_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5064959.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9505..10788)
                     /locus_tag="ACG81C_001807"
     CDS             complement(9505..10788)
                     /locus_tag="ACG81C_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5064960.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11471..12154)
                     /locus_tag="ACG81C_001808"
     CDS             complement(11471..12154)
                     /locus_tag="ACG81C_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5064961.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12172..12789)
                     /locus_tag="ACG81C_001809"
     CDS             complement(12172..12789)
                     /locus_tag="ACG81C_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5064962.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12799..13623)
                     /locus_tag="ACG81C_001810"
     CDS             complement(12799..13623)
                     /locus_tag="ACG81C_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5064963.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13616..14176)
                     /locus_tag="ACG81C_001811"
     CDS             complement(13616..14176)
                     /locus_tag="ACG81C_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064964.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14350..15306
                     /locus_tag="ACG81C_001812"
     CDS             14350..15306
                     /locus_tag="ACG81C_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5064965.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4495 a         3417 c         2622 g         4778 t
ORIGIN      
        1 ttgctattct attttaattg aattttgcaa ctcactcttc tttattccaa acctactcgt
       61 ttgaatatca cgtcaacgtt tttcaaatga tgatgataat caaaggcatc gtctatttct
      121 tctttagata ataccgatgt gatggttgga tcttcttcta acagcgggcg gaactgcact
      181 tggttgtccc aagcgtacgc agtttttggc tgaacaagat cataagcttc ttctcttgtc
      241 attcccttat cgatcaattt cagcataacg cgctggctat aaatcaaacc aaatgttgca
      301 gacatattcc gcttcatgtt ttcaggaaag accgttagat ttttaacgat attgccaaaa
      361 cgatttaaca tgtaattcaa taaaatcgtg gtatcaggca agatgattcg ttctgctgaa
      421 gaatgagaaa tatctctttc atgccataat gtgacatttt cataggcagt gaccatgtgt
      481 ccgcgcacca ctctggctag accagccata ttttctgaac cgatcggatt ccttttgtgc
      541 ggcatagcag aagaaccttt ttgacctttc gcaaaaaatt cttctacttc acgtgtttcg
      601 gatttttgca atccgcgtat ctctgttgca aaacgttcaa tacttgtagc aataagcgcc
      661 atcgctgaga aatattcagc atgtagatcc cgagggagaa cttgagtaga gatttcttgt
      721 ggacggatcc ccaattgttt acatacatac tcttcaataa atggcgggat atttgcgaac
      781 gttcccactg ctcctgagat ttttcctgct tctactcctt ttgccgcatg ttcaaatcgt
      841 tcgatgttcc gtttcatttc tgaataccat gtggcaagtt tcaatccaaa agtagttggt
      901 tcagcatgga caccgtgtgt ccgacccatc atcactgtat atttgtgttc ttttgctttt
      961 tcgccaatga tttccgtaaa acgtttcaag tcttcccgta aaatatcatt tgcttgcttg
     1021 atgagatagc cataagcagt atctacgaca tccgtacttg ttagaccata atggacccat
     1081 tttctttctt ctcccaatgt ttcagaaacc gctcgtgtaa acgcaacgac atcatggcgt
     1141 gttaattttt cgatttccaa gatccgatcg atatcaaatg atgcatgctc acgaattttt
     1201 ttcacatctt cttgcgggat ttcaccaagt tctgcccaag cttcatctgc taaaatctcg
     1261 acttcgagcc acgcacggta tttgttctct tccgtccaga tattccccat ttcagggcgt
     1321 gtataacgtt ctaacatgtt ttttctcctc taatcccaaa tattcgtttg tttgatttct
     1381 tccagcgttt gctggatgtc ttctgtcaag atagtgatat gtcccatctt gcgacctttt
     1441 tttgcttgtt tttttccata gaaatggaat tgccagtctg gcttattctc aatcaagcga
     1501 tagctttcca gcagttgttc tcccaaaaca ttgaccatta ctgcattgct tatcaaacgt
     1561 actttttctc ctaatggcca accacaaata cctcgaatat gggcatcgaa ctgactcata
     1621 gaacaagctt caatagaata atgtccagaa ttgtgaggac gaggagctaa ttcattcaca
     1681 tatagcccgc ctgtttttgt taaaaacatt tctaccccta aaacacctga aaggctcact
     1741 gcttctgcaa tcacacgggc aatacgctca gcctcctcga tcacatcctg atcaatcgcg
     1801 gctggagcaa tcgtttcatg aagaatattg ttgtggtgga tgttttccac gaccggaaaa
     1861 gtcgtgaaat ctccttgacc atttcctgcc accatgatcg aaatctcttt ttcaaacggg
     1921 atccatgctt ctaaaacaca ggttccttct cttaaaaggt ccatggcagg agctagatcc
     1981 gcccgacttt tcaaaacata ctgccccttg ccatcatagc cgcctcgagt ggttttcaaa
     2041 acacaaggat agccgatccc gtcaatcgca tcttggatat ccgtcggact gacgatcgtt
     2101 gcataaggag caatcacgat gttattcgtt tccaaaaaag atttttctaa caggcggtct
     2161 tgcgtaatcg caagcaagtc tgtcccttga ggaataaatg acatgggtaa aatcgcattt
     2221 aacgcctcta cactgacgtt ttcgaattca taggtaatga catctgttct tctggcaagc
     2281 tctttcagcg caaaggtgtc atcatagaca ctttcaatgt gccaatctgc aacttgtgcg
     2341 gctggacaat cgctcacagg atccagcaca cctactttaa agcccatttc ttttgcacta
     2401 atcgccatca tacgacctaa ttgaccgcca ccgacgatcc cgatcgttga accaggcaac
     2461 aaaggtttaa ccaagttgat cactactttc tataacagtt ttagccaaca ttgaacgtct
     2521 ttcagccaat ttagcctcca attctaaatc atacatggaa agcatctgta tcgcaagtag
     2581 tccagcatta attgctcccg cttttccgat tgctgtagta gctacaggta ctcctccagg
     2641 catttggaca atcgaaagaa gcgaatccaa accatttaat gttcgtgatt ggaccggtac
     2701 accgataacc ggcaaagttg tttttgatgc gaccataccc ggcagatgcg cggctccccc
     2761 tgctcctgca ataatgattt tgattccttt ttttcgagcg ttttgtgcat attcaaacat
     2821 caaatctggg gttcgatgag cagaaacgac ctttttttca taagacacac cgaattcatc
     2881 caatatttga catgcttctt tcattgtttc ccaatctgaa gtactcccca taatcacaga
     2941 gatttttgct gtcattgttt ttcctcccag tttttctaca ttcatatttt aacaatcact
     3001 ttttttgatg tcaaagaaat ttcgaatgtt aacaaagaaa aacattttat tgttcgtgtt
     3061 tattaaagat aaaaaaaagg aatgtgacaa aagttaggtc acattccttt ttatggataa
     3121 acggtgttct cctgccaact cctcggtcac aaggaacaat attcaacaaa catgagaagc
     3181 ttttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttcct
     3241 tcaacggtgt tcaatcagct gaccctcggt attagctcaa acaaacggca aaatacgagg
     3301 aactattttt ccgtttgttt ctctaacaat caggtcaaaa cgctgatttc acaacctttc
     3361 ttttgctcta ttacttcttg aaggcttctt ccaatatttc ttcatctttt ttcaatccat
     3421 taaagatcaa attcaatatg atagccgtaa tgctactcat tacgatgcca tttccagtga
     3481 acatttgcaa tgtttgcggc atttgactga ataatgtcgg cgtaacattg aaacccagcc
     3541 caaatccaac agaaacagca acaatcaata gatttttatc attgctgaaa tcaacttcca
     3601 acagcatacg gattccttgg actgccacca tcccgaacat gactaacatt cctccaccaa
     3661 gtaccggctc agggatgatt tgtgctaacg caccgatttt agggaataat cctaaaacaa
     3721 tcaagaagaa tgcagagaaa tagatgggtt ttcttgtttt gattcccgaa agttgaacca
     3781 atccgacatt ttgagaaaag ccagtataag ggaatgtatt gaaaataccg cctaagataa
     3841 cggcaagtcc ttctgcacga tatccctttt tcaattcttc ttctccaaca tttttcccag
     3901 taatgtcacc aagtgcaaaa tatacacctg ttgattctac catgctgacc atggaaatga
     3961 tgatcattag taaaatcgac catacgtcga aagttggttt accaaaataa aagggttgtg
     4021 gaacatggaa caacggcgca tctccaatcg cgctcagatc aatcattccc atgaaagcag
     4081 ctaagatgct tcctccgact aaaccaatca ataccgcgat tgaacgaatg aacccttttg
     4141 ccagcatttg cacgcctaca attaggagaa tcgtcacaaa tgcaagtagc agatttgttt
     4201 tgtcaccaaa atcagctgct gttgcattac ctccacccat tttctcgatt gctacaggaa
     4261 tcaaagtcaa accaataacg gtgataaccg ttcctgttac tataggaggg aatagttttt
     4321 taattttaga aaagacacca gaaactaaca caacaaagat cccagaagcg ataatagatc
     4381 cgtagatagc tcctactcct tcattggttc cgatcaagat cagtggagct actgcttgga
     4441 tcgcacatcc taaaacgaca ggcaagccaa ttccgaaaaa tcggttaact gtcaattgca
     4501 ataatgtcgc taatccacac ataaagatat caattgaaat caaataagtc atctgttctt
     4561 gattaaagcc tagtcctgta ccgatcaaaa gaggcaccgc tactgctcct gcatacatcg
     4621 ctagcagatg ctgcagccct aaaaccgcag cttttccatt ttgtgtttct ttttccacga
     4681 ttatgcgtcc tcctcaagaa actcaacttg tccattcgtc aaggaagcaa tacgcgcaag
     4741 agaaacgact ctcaatccca tttcttcaag taactgacgt ccatcttgga aagacttttc
     4801 gatgacgatc ccgattcctt ctacttgcgc tccagcttgc tggcataatt cgatcaatcc
     4861 ttttgccgct tgtccgtttg ccaagaaatc gtcgataatc aatactttgt cttctgaaga
     4921 taaaaattta cgagaaatag aaatcgtact tgtcacttgt ttcgtaaaag aataaacaga
     4981 agctgtcaat aattcttcat ccatcgtcaa actttttgct tttctagcga agatcattgg
     5041 aacatctaat tctcttgcag caaataaagc tggtgcgatc ccagaagctt caatggtcac
     5101 aactttcgtg attccttgtt ctttaaatac ttctgcaaac cgttttccca tttgttccat
     5161 caatacaggg tctacttggt gagtgacgaa gctgtcgact tttaatacgc cctcgcctaa
     5221 tatacgtccg tctttttgaa tacgttcaac taattctttc actttatttg ctcctttatc
     5281 atttattaat accgaaataa ccgcttttgt gcaaaaacct actatgtttc tgttaaagaa
     5341 aaacaccctc cactttatca taagaaaaga agggtgaaat agccaatctt ttacttatag
     5401 tccagcattt acggtactgg ctagaaactg tcatccatat tatgacgata tataagtagg
     5461 tatgtaaaac taataatagt gtaaattcat tttcttgcaa ctgttttgca cgtgaaggca
     5521 gtttgccata tttgaataaa aaatgttgtt tcaaatattg aaaacagtct aacactactt
     5581 tctgaaactg acaacgtggt ttacttattt ttttgtaaaa tctaatgatt tttaatgcat
     5641 tattcctttt tgttcgggtt taaatagtta tttttatttt ctcctatcta aaaaagcagc
     5701 ccacataaaa agaaggcatg acaaaaagca tgtcatagcc cctttttccg aataaatagt
     5761 gttcaaaaga cagcctatcg gcaataaggc aagattttat aaactacggg aagtaatttc
     5821 cgtaaatcta ttcttattct tttaagctga caagctcgtc aatcactact gcttttcctg
     5881 aagcaataga atgatttgct gcgattcctg tcaaaatcga caaaatacca tctacgtgag
     5941 ttgctgcccg attccacgga tcttcttctt tttcttcacc aaatagatca tctaataaac
     6001 gcggatctcc tccgccatgt ccgcccgctt cttgttcgac gattacctca tatggctcac
     6061 cgaacatcgg atagacatgg atggactgaa gaacggctgc tccttcatcc tttttatctc
     6121 cacctgcatt gatgtaagac ttttcgatca ctttcatttc gatccgtcct ttgctgccat
     6181 tgaaagaaac attgaatcct tcccacggca tatacgcgtt tagcgaatac gtcaaaatcg
     6241 caccagtttc atatttgacc gttacgccta gtgtatcttc aatagagata tcatcggaaa
     6301 atacactgcg gtcacgaata tatccactgt ctttttctgc ttctaaatac atttctcgca
     6361 attcctcatt ggctgacagg tctatagcaa atggatcagc tttagctagt tcgcttccat
     6421 agcatcgact gtaaaatatt tctactccgc gtttctccgc attatgcatc ccataaaagt
     6481 ttaatccacc gaatgcaaat accttttggg gacggctgtt caaccaaaaa ttcactagat
     6541 caaaatgatg ggtagattta tgaacaagaa gcccgccgct attgtgctta tttcgatgcc
     6601 atcttctgaa ataatctgcc ccgtgttgcg tatttaacag ccattcgaag tggatagaaa
     6661 acacatcacc aatcacatca ctttctatca gttcctttat cttcgtgtga tacggtgcat
     6721 aacgataatt aaaagtgact cgcaggcttc ttcccgtctg tttttggacc gttattattt
     6781 cttgagcttt ttcctcatcg atagtcagag gtttttcgca aataacatca tatccttttt
     6841 tcattgcacg aatgatataa tcatgatgcg ttcgatctac tgacgtaata atgacttcag
     6901 ccggccgtac gtcatccagt agttgctcaa attgtgatgg catacatgtc tttacttttt
     6961 tctgtccata cttgttcaca agtatccgat ttgcataatc catccttgtt tgagatacat
     7021 cacaaaaacc gactagttca caagtttgat gatacgtaga ggcaattgcc tcataaaaaa
     7081 aacgcgctct cccaccaagt ccgacttgta catatttttt cttcatttaa agtcattctc
     7141 cttttctttt aagcaaaata ctgtgacaaa tggcagccct ggatacagta atgcagtgag
     7201 taaaaaatca tgctgtatac accagaacag tccatacaaa aggcataaat accacagaaa
     7261 ccccttgacg ttttttgaca acagccagat attccttttg atcaaataaa caagactatc
     7321 tgctttatct tctttgcaga tatcaattag cgtactaaaa aaactatggg atacaaaaag
     7381 caacagacac aatagaaaag gaatcagata ccaagctcca atctttccca ctaataaata
     7441 ccaatcaact gcaaaaataa tgacaaacag ctgaaacaac gtaatgatga aacacatccg
     7501 tttgattttt cctttcctcc aacatacctt tttttctgcc aagtaatgga cagatagata
     7561 ataagaagga tacatcatca aaaaataaat cagatagaca acacgagcag acagcactaa
     7621 tcctaaagtg aacaaacatc ctagaactaa agcgaaaaga gcattactga taagactggt
     7681 taaaaaaatc cgacagacat agtagttgag cttgtaaaga aacatctctt ctattggctt
     7741 tttcatattc agcccttcag accgcttgta ctgatcccct ggacaagata tttattgaag
     7801 aataagaaaa tcagaaaaac cggtagcagc gacaacgtag acatcgcaaa catactcgcg
     7861 taagtcgtac tagatccagc gtctgcaaat aatttgatgg caatcgatac cgtgtaagat
     7921 ctcggcttgt ttaaataaat caatggtccc atgaagtcgt cccatttcca ataaaactgg
     7981 atgatgatcg tcgtgatgat cgaaggtttc agcaaaggaa aaataatttt ggtaaagaca
     8041 ctatacttgc tgcatccgtc aataatcgct gattcatcta actctttggg gatgctgacc
     8101 atgaattgca tgatctggta aatgaaaaat gcttgtccaa aaaaatgtgg taaaatcagc
     8161 ggcacatacg tcccaaccaa attcagccga ttgtaaataa taaactgcgg aatcaaaatc
     8221 acttgtgcag gaatcatcat cgttactaac atgacgacga accagaatct ttttcctttg
     8281 aaatcgattc tcgataatgc gtaagcaata caagtcgagc tcagaactgt ccctatcgtc
     8341 gaaactgcag agatgacaaa cgaattccta aagaaagtaa aaaatgaaat tccagcaaac
     8401 cccgcccatc cttttgcgta attttcccat tgaaaagtct ctggaatcaa attcagcgaa
     8461 ccactcataa tctcttgatc cgttttaaaa gaacctgcga tcatccataa gaccggataa
     8521 atcatgacta gacctaatag caaaataaaa ccgtattgaa accatttcgt tactgtatta
     8581 ttcatttctt cctcctctca ttcacacaac gacccatttt tttgatgatt tgaaaatcag
     8641 aaaggtgaca gctcccataa tcaacagcat gatccacgaa atagcactag cataccccat
     8701 agaattaaaa gaaaaagctt ggttatacac ataaagagca taaaagttcg tggcatcatt
     8761 cggaccacct tgggtaatga taaatgcctg cgtaaaagtc ataaacgccg cgattgtctg
     8821 cataaccaag ttatacaaaa tgatcggcga aagaagaggt aacgtgatct tgtaaaacgt
     8881 ctgccatttg ttcgctccat cgatctctgc cgcttcataa tacgagcctg ggatctcttt
     8941 gatacctgca gcaaagatga tcattgccga accgaattgc caagcagaca tcaaaacaag
     9001 agggatcatg accagttttt gatcgctaaa ccaggaaagc gcaggtgcgc ctaatcgttc
     9061 caggaaaata ttcactaacc cgtttttaga aaacaactcc cgccaaacta gagcgataga
     9121 aatactgcca ccgatcaaac taggcacata atacaaagaa cgaaagaaag tcgttccttt
     9181 gatattcctc gttaaaagat aagcaatcaa tagaccaaag atcagtttca caggtacaga
     9241 tgcaaataca tagagaaaag tgactcggat ggagttcaaa aaacgggcat cactcagcat
     9301 ctttatataa ttatccaagc cgatccatga gactggattt cccaaacgat aatcggtgaa
     9361 cgaataataa agtgacaaga tcatcggtat cactgttatc aatagaaatc ctaggatgaa
     9421 tggagaagca aatacgtaac cgaccacatt gtcttgatcc aagaaccgtt ttagtttttc
     9481 ttttttcatg ccttcctcct gctgctatcc tagaatgttt gacgcaaact cataaagact
     9541 ctctgctgct tcttctgctg ttacttcttc ataaatgact ttttcaacta aaagattgta
     9601 ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc cgttgtccat
     9661 atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat ctgcacgagc
     9721 gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta aaatttcatt
     9781 ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt tatgggctga
     9841 gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt ctccatctgc
     9901 ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc ctgacaatat
     9961 ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc taaccgcctg
    10021 aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg cccctttgtc
    10081 taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt caaatcccaa
    10141 gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt gtggtatttg
    10201 aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc ctaattcttc
    10261 agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag ctggttcagg
    10321 gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat tcaccccgtt
    10381 agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt catctgcatt
    10441 agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct gattgatgta
    10501 ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg ctgctagtgt
    10561 acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg tcacatgggg
    10621 atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga cttgcgaacc
    10681 ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag ccgtcaagaa
    10741 gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc tctgcacctc
    10801 tacttccatt gttatttttc aatatactgt atttttatac agaacatatt tattaaacga
    10861 tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag gatgaacaac
    10921 tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta aaaacacttc
    10981 ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac aactgcccaa
    11041 tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct aaaaaacaga
    11101 aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg cacaagcatt
    11161 gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac aatttagctg
    11221 ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta tagaaagcgc
    11281 ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg ttttaactct
    11341 tcttatgtac attacaggta aaaaaacaaa aaaatttctc ttttttttga attctgaaca
    11401 aatagtactg gagtacaaaa gacgaggaag agccactatc tgctcttcct cgtcttttga
    11461 ttctcacctg ttattcatga ttctttttct cagtttcccg atatttttcc attttacgac
    11521 taaaaagctc tccgctactt ggtggcgtat atgtgatggc atttgctcct gcttgaatgg
    11581 tctcagcaat actttcttcc gttggaccac ctgtcgcaat gataggaagt tcaggatatt
    11641 ttttacgaaa atggcgtact gcttctgcag tttctttacc tgcactgata ttgataatcg
    11701 aaataccagc tgctaacttt tcatctattt ctgtatattt cgacgttacc gtactgataa
    11761 ctggaatatc aactactcga caaacttctt ttactgtctc taaaggcgta ggaccgttca
    11821 aaacgacccc taacgaacct tgcgcttctg cgaacatgct catgtaggaa gaacgagcac
    11881 cttgtgtcaa accaccacct acaccggaga aaactggaat gtcagctgcc tcaataatgc
    11941 ttttagtaat cgctggatga ggtgtaaaag gatacacagc aattactgca ttcgcatttg
    12001 tattacgtat gatcgcaata tccgtagtaa agatgatcga acggatcaat tttccaaata
    12061 tcttgatccc gcttgcttct aaaatgattt ccggtactct tacgatatct tttctcaatt
    12121 ccgtcatgat ttctggcgta aattgttctg ccatcagcca cttctccttt gctatctccg
    12181 gtcatactga ttcaattgat acgtctcgat gacctggatg attttttgcg cataaccggg
    12241 gtctgtcgca taacctgcat cttgcaatgc ttgtgcggct tcctgatagt tggtggccgt
    12301 aatcactttt tcatacagcg caggattcca atctactcct tggacgaaca gttgtgtatg
    12361 gtcatccatg gactgttccc atgaatcata cactttgaag tctccttgga tagttatcca
    12421 ttcttcgtta acaaattcct ttgtttccag gcttactttc ttctgatccc cataagcctt
    12481 gatcccgaaa agattattgt atttgctagc caaggtactt tttccccaat ttgattctaa
    12541 tatagcttgt cctaaaatga tactaggaag cactccataa ccatcttgta gttcttgggc
    12601 atgcggaaca agccgttcaa taaattgctg atgggattgc gcttcatttt cttgctgatt
    12661 atcagtaaaa ggagaagaca gattcttcaa ggaaaaaaca aaggctaaga gtatcactgc
    12721 tacaccggca accactgctg gccaacggat attcttgcgc ttttgctttt tccacttttt
    12781 tttcgccatg atcaattcct acttttcatt taattcattc agatattctt ctagcgtcaa
    12841 ttggtgtttg gtgatatact cagcgttttc tttaccgaca taacgtaagt gccaaggttc
    12901 atatgtgatc ttagttatat cttcgtgtcc ttttggataa cgaacgataa atccatactg
    12961 cgcagcatta tctgcgatcc atttagctcc aggctgatct ccgtaacttg catccaatac
    13021 agtagaagga taactgttgt accagttttg atcgacgaca tcgactgcca gacctgtgtg
    13081 gtgttcgctg tatcctggct ccgtaatcgt ttctttagta atctttgtcg cctcctcttc
    13141 tgatacacct tgacgactca tcacatcttg gacattttgt tcgaaaactt gttgttggga
    13201 tgatacagac cggtaagctg agaccatcac taatggaaat ccagctgctt cagcagcctt
    13261 ggcaaatgct tcatattctg gagcaatgcg tttgtcgatc aagtatccgt tacttaatga
    13321 tgccaattga gtagactcat caatttcttt ttctaatttg tgatctggac caaccaaaac
    13381 tagcttccaa tcgtctgttt taacatctgg caattgtttg gacgtacgat cttttgtttc
    13441 tttgtccttt accttgctgc tggttgtttg actgttcttt tctttactag cagttgtttt
    13501 ggtgcttgtt cgttcgactg cttgattgcc ggcttgaaca tgcgaatcat catcgctaaa
    13561 aataacatag cccattgctg cgatcataat cacaactgct ccaaatccta aaaatttatt
    13621 cacttgaaaa atcctttcgt tgttcttcac gttccccatt cgcctcatga acgtaagttt
    13681 cttccagatt ttcattcacc caatcgagaa gttcgatttt ttctccttcg atctgttttt
    13741 gaacatctgc tggcagacga aaattcaaaa tatcatcata gccccattca tcatattgaa
    13801 tacttacttt caagtcaagg tatctaccag cctgctgatt atttttcaaa gggatcagct
    13861 cgacttttgc tgctttgctg ctaagccatt tttgcgcgat gcgtgttttg atttgcttgt
    13921 actctgcaac ggcaacgttt cgtttttcgg gaaaaataat cgtttgacgc aaaatctttt
    13981 gcgttagcat ccattcataa tcagtgaaaa gattttttac tttctgcatc gcttcagatg
    14041 gcagattttt ctcgccatct ttccattttt gccattgttc tggactgatc agcaagtatt
    14101 catctaaaaa atttttttca gtttcaaact gttctaataa tacactcata ataagatcaa
    14161 tatatacttg atgcacgttt atcccctcct tttaactatt ctacttaagt tttgatcaaa
    14221 aagcacctct taaatcgctg ctttcatttt ttttaatgtt tcaagtcctc tcctctttat
    14281 tcttcgtgat ttttgttata ataatagaca gacaagaaac tcaatccaac tatgaaggag
    14341 cgttttcaga tgaaaaaata tcaatgggga atcgttggtc ttggtaccat cgcacatgag
    14401 tttgccgaaa gcttcaatca agagacgagc gaactaacag cagttgcttc cagaacttca
    14461 gaaaaagcgg aaaacttcgc tcaccgttac aatattccaa aagcttacgg aagctatcaa
    14521 gaaatgttag acgatgctga aatcgacatc gtgtacattg ctgtaccaaa tagacagcat
    14581 atcgaccata tcttggcagc attggaagca ggcaaacacg ttctatgtga aaaagccatc
    14641 acgatgaata aaaaagaact ggctgacgct atgcgcttag cagaagaaaa gaatttgatc
    14701 ttagcagaag cgatgacaat cttcaacatg ccactttatc agcagttacg ctcaataatg
    14761 gatacaggaa aactgggggc tttaaaaatg atccaagcac ctttcggcag ttacaaagaa
    14821 ccagacccta aaaaccggtt cttcaatcca gaattagctg gaggagctct gttggatatc
    14881 ggtacgtatg ctgtatcttt cgctcgtttc tttttgagct ctcaacctga agtagttgcc
    14941 tctacaatgg ttccttttga aacaggcgtg gacgaacaat ccgtcactat cttgcgtaac
    15001 aaagaaaatg aacttgctgc tgtcagcttg acttttcagg caaaaatgcc aaaagtcggt
    15061 atcgttgcct ttgagaacgg ttatgtgacg attgctgatt acccgcgtgc tgatcgtgcg
    15121 gagatcttat ttacagatgg tacaaaggaa ttcatcgaaa gcgggaatac atctgaagca
    15181 atgaattacg aaataaagaa tatggtacac atgattgaag gtgacttgcc caatcgctct
    15241 ctcttcttga caaaagatgt catcgaaatc ttggatcaaa tgcaagttct atggaaaaat
    15301 atgtaatttt aa
//
LOCUS       DAZUMI010000067        15255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000067 DAZUMI010000000
VERSION     DAZUMI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.067"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACG81C_001813"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACG81C_001813"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5064966.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG81C_001814"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG81C_001814"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5064967.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACG81C_001815"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACG81C_001815"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5064968.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACG81C_001816"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACG81C_001816"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5064969.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACG81C_001817"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACG81C_001817"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5064970.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG81C_001818"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG81C_001818"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5064971.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACG81C_001819"
     CDS             6726..7466
                     /locus_tag="ACG81C_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5064972.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACG81C_001820"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACG81C_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5064973.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG81C_001821"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG81C_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5064974.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACG81C_001822"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACG81C_001822"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5064975.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACG81C_001823"
     CDS             11260..11898
                     /locus_tag="ACG81C_001823"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5064976.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACG81C_001824"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACG81C_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5064977.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACG81C_001825"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACG81C_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5064978.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="ACG81C_001826"
     CDS             13675..15249
                     /locus_tag="ACG81C_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5064979.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5114 a         2864 c         3319 g         3958 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatga
//
LOCUS       DAZUMI010000068        15137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000068 DAZUMI010000000
VERSION     DAZUMI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.068"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG81C_001827"
     CDS             4..2376
                     /locus_tag="ACG81C_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5064980.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG81C_001828"
     CDS             2437..3897
                     /locus_tag="ACG81C_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5064981.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG81C_001829"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG81C_001829"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5064982.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG81C_001830"
     CDS             6128..6277
                     /locus_tag="ACG81C_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064983.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG81C_001831"
     CDS             6352..6945
                     /locus_tag="ACG81C_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5064984.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG81C_001832"
     CDS             6958..7857
                     /locus_tag="ACG81C_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5064985.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG81C_001833"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG81C_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5064986.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG81C_001834"
     CDS             8723..8983
                     /locus_tag="ACG81C_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064987.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG81C_001835"
     CDS             9428..9634
                     /locus_tag="ACG81C_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5064988.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG81C_001836"
     CDS             9655..9885
                     /locus_tag="ACG81C_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064989.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG81C_001837"
     CDS             9901..10338
                     /locus_tag="ACG81C_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064990.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG81C_001838"
     CDS             10331..11038
                     /locus_tag="ACG81C_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064991.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG81C_001839"
     CDS             11202..11402
                     /locus_tag="ACG81C_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064992.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG81C_001840"
     CDS             11418..11597
                     /locus_tag="ACG81C_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064993.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG81C_001841"
     CDS             11922..12185
                     /locus_tag="ACG81C_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS5064994.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG81C_001842"
     CDS             12179..12529
                     /locus_tag="ACG81C_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5064995.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG81C_001843"
     CDS             complement(12553..13167)
                     /locus_tag="ACG81C_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5064996.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACG81C_001844"
     CDS             13481..13903
                     /locus_tag="ACG81C_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064997.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACG81C_001845"
     CDS             13913..14116
                     /locus_tag="ACG81C_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5064998.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACG81C_001846"
     CDS             14327..14911
                     /locus_tag="ACG81C_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5064999.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3060 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaa
//
LOCUS       DAZUMI010000069        14815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000069 DAZUMI010000000
VERSION     DAZUMI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.069"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG81C_001847"
     CDS             complement(296..1024)
                     /locus_tag="ACG81C_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065000.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG81C_001848"
     CDS             complement(1002..2576)
                     /locus_tag="ACG81C_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5065001.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG81C_001849"
     CDS             2805..3743
                     /locus_tag="ACG81C_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065002.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG81C_001850"
     CDS             3755..4666
                     /locus_tag="ACG81C_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065003.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG81C_001851"
     CDS             4690..6162
                     /locus_tag="ACG81C_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5065004.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG81C_001852"
     CDS             6213..6824
                     /locus_tag="ACG81C_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5065005.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG81C_001853"
     CDS             6854..8767
                     /locus_tag="ACG81C_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS5065006.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG81C_001854"
     CDS             8895..11876
                     /locus_tag="ACG81C_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS5065007.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG81C_001855"
     CDS             11873..13534
                     /locus_tag="ACG81C_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS5065008.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG81C_001856"
     CDS             13574..14506
                     /locus_tag="ACG81C_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5065009.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZUMI010000070        14813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000070 DAZUMI010000000
VERSION     DAZUMI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.070"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(290..856)
                     /locus_tag="ACG81C_001857"
     CDS             complement(290..856)
                     /locus_tag="ACG81C_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5065010.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(856..1812)
                     /locus_tag="ACG81C_001858"
     CDS             complement(856..1812)
                     /locus_tag="ACG81C_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5065011.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1812..2468)
                     /locus_tag="ACG81C_001859"
     CDS             complement(1812..2468)
                     /locus_tag="ACG81C_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5065012.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2838..4856
                     /locus_tag="ACG81C_001860"
     CDS             2838..4856
                     /locus_tag="ACG81C_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5065013.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4926..5132
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5283..7697
                     /gene="leuS"
                     /locus_tag="ACG81C_001861"
     CDS             5283..7697
                     /gene="leuS"
                     /locus_tag="ACG81C_001861"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5065014.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8045..9598
                     /locus_tag="ACG81C_001862"
     CDS             8045..9598
                     /locus_tag="ACG81C_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5065015.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9979..11763)
                     /locus_tag="ACG81C_001863"
     CDS             complement(9979..11763)
                     /locus_tag="ACG81C_001863"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065016.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11778..13499)
                     /locus_tag="ACG81C_001864"
     CDS             complement(11778..13499)
                     /locus_tag="ACG81C_001864"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065017.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13599..14360
                     /locus_tag="ACG81C_001865"
     CDS             13599..14360
                     /locus_tag="ACG81C_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5065018.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4815 a         2727 c         2938 g         4333 t
ORIGIN      
        1 cttatatatc aatgtttttg ccattctttt atatatctgt tatatgtcaa aaaaatgtgt
       61 tcaatttcaa tttgtatttg aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
      121 aagaacagcc aatcaaaaac agcttctcat gtttttgatt tggttggact tattaccgca
      181 gatataagct tttgagcacc gtgaataaag aggttatgaa atcaacgctc taacctgagt
      241 attagagaaa aaaacgcaaa acatatcatt tatgttttgc gttttttatt tatctttttt
      301 tctcaataca atataaaata ggcggctggt tcttttggtt gataaaccga taattcagca
      361 cattatatgt ttcctgtggc agtttggaac aaaaagaatc aaccatatcc agttcttttt
      421 ctccaccttc atgaccatag tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt
      481 tcaatatttc ttccatagct gtgcgtgtag tctctggcat agtgatgatt gctttatcac
      541 tttttgggag ataccctaga ttgaaaatcc ctgctttgag attttgttgt tcagctatca
      601 cttctcctaa cgtttcgtgg ccttgtagaa acaatgagac acgttcttct aactgacgct
      661 gttctagcct ttcacgagta gcatcgattg cttgttgctg gatatcaaaa ctatagacat
      721 gaccagtttt tccgatgtgt tctgctagaa aaaccgtatc gtggccattg cccatcgttg
      781 catctacgac gtgatcccct ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca
      841 aagcagtttg caacatcaga cgctcacctt tttccgtaca tcatattttc cttggaagct
      901 atctctgcga agaagttctt catcaattgc attcaagact tcccattttt tcaagctcca
      961 cattggtcca atcaaggaat cccacggcgc atcccctgtc aaacgatgga tgatgatctc
     1021 ttttggtatc atttccagct ggtcgcaaat gacattgaca tagtcaggac ggctcattaa
     1081 ttgaaggcga ccttcatgat agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa
     1141 atggagcttg attccttgga tatctgaatc taaaatggtg cgtctgacat tttctttcat
     1201 catttctaaa ctttcgcctg gtaatccgtt gatcaaatgc gtacaaacac ggatattatg
     1261 tttgcgtaat ttagccacgc catctaaata agtttgataa tcatgtgctc ggttgatcag
     1321 ttttgaagtt tcttcaaaag ttgtttgcaa tccaagttct acccataaat acatccgctc
     1381 gttcagttca gcaaggtaat caactacttc atctggcaga caatccggtc gtgtaccgac
     1441 tgataatcct acgacacctg gtaaattgac tacttgctca aatcgttctt tgattatttc
     1501 aactggcgca tgggtgtttg taaaattttg gaaataaacg atgtattgtt ggacatgagg
     1561 ccatttttga tgcatcatgt cgatttcttt ctggaattgg attggaagag ggtcttcagg
     1621 tgcaacgatc atgtctcccg aaccagatac actgcaaaaa gtacaaccgc catgggcaac
     1681 agtcccatct cgattcggac agtcaaatcc tccatcgatc ggcaccttga aaattttccc
     1741 gccaaactca tttcgtaaag cgtaattcca agaatgatat cttttatttc cttctgaata
     1801 aggaaaagtc attaaaagtc cttctttctg taagagttat ctatttgttt tcttttttct
     1861 ctatacactg gataagtcag gcacagccaa gaaagactga gtaagtagcc gccgatgata
     1921 tcagtaggaa aatgaacgcc cagataaatg cggcttatcc cgacacttac tataataaac
     1981 atcatcagca attgaagcaa tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga
     2041 aatagtaaag aaccgtaaag aatcatactg cctgctgcat gtccactagg gaaactgtaa
     2101 cttgtttcaa ctactaagtg cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc
     2161 accgggttga tgattccagc aattacaata aatccagaaa cgaaccaaaa tgtttctgcc
     2221 ctttttttct tataccacat ccataaagcc gcacaagcaa ataaaatgat gacggtaaga
     2281 ggattaccga atttagtcat aaagagttgg tagctgttca gatcaggata agaagctctg
     2341 acaatgcctg taaaccactg atcgaaacca gaaagccacg acagatggaa ctttacgatc
     2401 cagcccaaaa tgaaaaacag gacaagaaag aagctaccag ccacctgaaa tgatgtttta
     2461 tatttcatac aattcctcct ttgaagcaat gacttattat agcatttttg gtccagcgaa
     2521 aaaaacggta aagagataaa accaatgtat gattcctctt tgaataacca atattcttat
     2581 aaaaaaaata actgtaatcg gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa
     2641 ataaaatcag tgtttttcga aatcctttta aacaagaacg tagacgtagg aagaacttga
     2701 aaatgaacag aagttgttca ttacggtaaa ttacaatatg attacaaaaa acaacaaagc
     2761 tcttaaataa cttttaataa atattaagag aagttataat tattaataat agataaattg
     2821 aagttggagg atagaagatg gaaaacattg caagaaaaga acggagacgt ttagaacaga
     2881 ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt gggaactgcg atggtaggct
     2941 gttctgtcgc tgccccatta gtacagccgg ttcctgttca agcggatgaa acacctgttc
     3001 agttcggcgc aagaatcaat acagcggcat ttatcacaga aattgctact tatgctcaac
     3061 caatcgctca agccaacgat ctatatgcat cagtgatgat cgcacaagca gttgtagaaa
     3121 gcggttgggg cagtagtgcg ttatctcaag caccttatta taatcttttt gggataaaag
     3181 gcagctatca aggacaaacc gtctacatgg atacgcttga atttttaaat aatcaatggg
     3241 taaataaaaa ggagcctttc cgacaatatc cttcttttgc tgaatctttc aatgacaatg
     3301 cctatgtact tagaaatact tcatttggca acggttatta ctatgccggt acttggaaga
     3361 gcaacacgaa atcctataca gatgcgacag cttgtttgac agggcgttat gcgactgatc
     3421 ctggttacgc aggcaaatta aataatatca tcgcaactta cggattgacc caatatgata
     3481 caccagctag cggaaacgca ggcggtggag cgactacagg tggcaataat actggaagtg
     3541 caggtagtac tggcggttca agcaattctg gtaatacaaa ctcaggttca gcaacttcga
     3601 ccacttatac tgtccaatct ggtgattctg tttggggaat ctcaaataaa ttcggtatca
     3661 caatggatca attgatccaa tggaacaaca tccaaaacaa tttcatttat ccaggacaaa
     3721 aactgacaat caaaggtagc caggaaaatg gctcttcgac gaataattcc ggtaacaata
     3781 ccaactcttc aggaaatgct ggtacatcaa acggaggaca aacaactggt gcgaaataca
     3841 ctgttcaatc tggtgattct gtttggaaga tctcaaatga tcacggaatt acgatggctc
     3901 aattgattga atggaacaat atcaagaaca atttcgttta tccaggacaa cagctaatcg
     3961 tatcaaatgg cggttcttct aatgctggag caaatactgg ctcaaatact gggaatacaa
     4021 gtggctctag ttcgtccagc tcaagttctt ctaatacagc ttcaggaacg aaatatacag
     4081 ttaaagccgg agactctgtt tggagcgtct caaataaata cggaattacg atggctcagc
     4141 taatccaatg gaacaatatc caaaataact ttatctaccc aggacaacaa ctgactgttt
     4201 caaacagtgg atcaacaaac agcacgtcag gtaatacagg ttcgtcaacc aatacaaata
     4261 acagcagttc agttaatact ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag
     4321 gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac gatggatcag ttaatcgaat
     4381 ggaataatat caaaaacaac ttcatttatc caggacagca attgatcgtt aaaggtggaa
     4441 cttcggcaaa tacaaataca ggttcaacta cttctgcagc gaaaccaaat acaccgaata
     4501 cgtctgctac gacaagtaca tcatccactg gtaatacgat gtataccgtg aaagcgggcg
     4561 agtcagtttg gagcgtagcg aacaaacatc atatcacgat ggatcagtta atcgaatgga
     4621 ataatatcaa aaataatttc atctatccag gacagcaagt gattgtgaag aaaggttcat
     4681 ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac ctatactgtg aaatctggag
     4741 aatctgtttg gagtgttgct gacagtcatg gaattacaat ggctcaacta atcgaatgga
     4801 acaatattaa gaacaacttt atttatccag gtcaaacgtt gattgtagct aaataaaata
     4861 gctgttcgaa gaaataacga ttgaattcca caaaaatatt tgttactata aggctactta
     4921 aatatgacaa cacttgaaaa ggaagtagta aaaagactga agtttcagag agcgcatggc
     4981 tggtgagaat gtgcacggat gcttttgaac tcgcctttga gttgcccttg ctgaataccc
     5041 gagtaagcaa gcggcggtga cggccgttac aacgttcgag gccttttcac taaggtaaaa
     5101 ttaggtggta ccgcgtagat tacgtcctat aaaaagagca atctttttat aggatttttt
     5161 tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa ataaaaataa acagattctg
     5221 ccggtttttt cgttatattt ataatagaaa caagtgtttt caatagaaca ggaggacagc
     5281 aaatgagtta caatcataaa gaaattgaga aaaaatggca aaaatactgg gcaaaaacga
     5341 atactttcaa cacccatgat gatcctgaaa agccaaaatt ttacgcactg gatatgtttc
     5401 cctatccatc cggtcaaggg ctacatgtag ggcatcctga aggttatacg gcaacggata
     5461 tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct gcatccaatg gggtgggatg
     5521 cattcggtct gcctgctgaa caatatgcgc tagatacagg aaacgatcca gctgagttta
     5581 cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc tttaggattt agttatgact
     5641 ggaatcgcga agtgaataca actgatccgg agtattataa atggacacaa tggatcttta
     5701 cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt agcggtaaac tgggtacctg
     5761 aattaggaac agttatttca aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct
     5821 atgatgtagt cagaaaacca atgcgccaat ggatgctgaa aatcactgct tatgctgatc
     5881 gcttattgga tgatctagaa ttagttgatt ggccagaaag cattaaagaa atgcaaagaa
     5941 attggatcgg tcgttcagta ggagcaaata ttgaatttaa agtggctggt acggataaat
     6001 cctataccgt atttacgaca cgaccagata ctctattcgg tgcaacttac tcggttcttg
     6061 cacctgaact agatcttgtt agagaaatta cgacccctga acaaaaagct gctgtagaag
     6121 cttatatcga agagacagct aaaaaatcag atctaaagag aacggatctt gcaaaagaaa
     6181 aaacaggagt atttactggt gcttatgcaa tcaatccagt taatggcaag gaaatcccaa
     6241 tctggatcgc cgattatgta ttatcttcat acggcacagg agccatcatg gctgttccag
     6301 cacatgatga acgtgacttt gaattcgctc aaactttcgg actagaaatt cttccagtta
     6361 tcgaaggtgg agatgttgaa aaagctgctt acacagaaga tggtacgcac atcaactctg
     6421 aatttctaga cggcatgaac aaacaagaag caatcgataa aatgaatgaa tggctagaag
     6481 aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg tgactggcta ttctcccgtc
     6541 aacgttactg gggtgaacca atcccaatca tccattggga ggacggaaca gtcacagctg
     6601 tcccagaaga agagctacca ttacggttgc ctaagacaaa aaacatcaag ccaagcggta
     6661 ctggagaatc tccattagca aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag
     6721 gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg ggcaggaagc tcatggtatt
     6781 acctgcgtta tatcgaccct cataacaaaa aagaactagc agattatgaa aagttgaagc
     6841 gttggctacc agttgacatc tacattggtg gagcagaaca tgcggtactt catttactat
     6901 atgcacgatt ctggcataaa ttcttatacg atatcggtgt tgttcctaca aaagaaccat
     6961 tccaaaaact atacaatcaa ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat
     7021 ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac ttacggtgca gatacactgc
     7081 gcatgtacga aatgttcatg gggccattag atgcttcgat tgcttggagc gaaaatggct
     7141 tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt gatcgttgat gagaacaata
     7201 aaatgcgtga ccgtatcact acattgaatg atggcaagct tgataaagtg tatcatcaaa
     7261 cagtgaaaaa agtgacagaa gactatgaaa atcttcattt caatacagca atctcccaat
     7321 tgatggtctt tatcaatgaa gcctataaag tggacgctct gccttatgaa tatatcgaag
     7381 gatttgttca actactcgca ccgattgctc ctcacatggg tgaagaacta tgggcaatcc
     7441 ttggaaatga tggtggtatt tcttacgcac catggccaac atacgatgaa gcagcacttg
     7501 ttgaagacga agtagaagtt gttttccaag taaacggtaa agttcgtgcg aaaagcaatg
     7561 ttccccgaga cttaggaaaa gatgagctag aaaaagcagc attagccaat gaaatagtgc
     7621 aagaatatat tgaaggcaaa acagtcagaa aagttattgc ggtaccaaac aagttagtca
     7681 atatcgttgc taattaattg gctctttgtc aataaggact gatgagttgt gcaaaatcaa
     7741 atctgggcca gaatgaactt cattctgtct cagatttttt gttatttcaa attgattaat
     7801 tgattgatca aaaaggtttt gattttcatg ttctcaaaca cgttcaatgt ctttttcttc
     7861 tgaatggtct tgtaagagat ctctgtaatc atcaaatttt tgtctattaa tcttagtatt
     7921 ttttgatgta taaactacct gaaattgaga gtacatacca aattcataaa ttttttatta
     7981 gtgggactag tgattgtatg gattttgacg cataatgaat gaagtatgag aaaaggagag
     8041 acaaatggat caagaactat ttattatcga agtattatca gctaaacaaa aaattatcga
     8101 gaaagcgaaa aaaagagcga aaagaataat gttcaagctt ttaagcttag tatttttctc
     8161 tggtttgctt tttttcggag ctgtatcgtt cctatatcgc catatgagtg cagtagtccg
     8221 ttcagctgaa gggatgcaaa aattaaataa tttgtatcac ttttcgtggg gagttggcgg
     8281 aataacaatt gttgtgctta ttgtttttct tctacttatt tggctccgtt accatcaatt
     8341 gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa cagtttttac ataataaacc
     8401 aacttctcaa gatgatgact atctttattt tgagaagact tcgacgagtc aagcttttcg
     8461 gatacaaaaa agtgattgcc agttattgct gagatcactt gatgatttac ctatctactt
     8521 aggcgaaagc aaatcaaaat tcggaatgaa cacattccat atttttttac tagaaaacga
     8581 acccagatgg gaaaaaagcc agtcttcgcc tggtggatac ctgacaacta agatgagtac
     8641 attagtcact ggttttttcc tcattctttt agctggaaca atctattatg ggagatacac
     8701 tcagccaaaa atgtcttctc atataaatgc tgcttctcac atgacaatga actcaactaa
     8761 cgatacgcat atacaaaaga caaatgaagg gttgttagct gatcctaagc aaaaggtaaa
     8821 tttgacaaca caaaagataa atgatctcaa acttgataca gagacgcact ctctctatat
     8881 gacaacagat agtggtgcta attgggtatt tgtgccgatt gattcgcaat ggttgcgttt
     8941 tggcgaatac accctgacaa cggggctgat tcctgaaggt tattggatgg acaacacata
     9001 tgatatttcc gccgatttct catggtttat ctattcttct ggggatgatg tacgtctgct
     9061 gacttcaaga gataatggta aaacatggca aacgtctatt atcacccaat cagttcagcc
     9121 gatacgttat cgaaaagcgg tattttatgg caacgatggt ctatttatct gtacatcagc
     9181 cagtgggatg tctgcagaaa ctttgtacat ttacactact atggatggcg gaatcacttg
     9241 gataaaaagt ggaagtacaa tgatcaatca gccagtacaa aatgtcagct tcgttacaac
     9301 ttctttaggt tttgtttcga cacgtgaaag tttgttttat acaaacaacg gaggacgctc
     9361 attcaaagaa tcagttctgt cgattccgga aaactatcag acagggggga tagatctttt
     9421 tcaatcacca aatgaagtta ctcaaataag cgcgaatacg ttagagacaa aattttattt
     9481 gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc tgcctcttcc gttcaactga
     9541 taacggggaa acatggcaat tcgttcagca actatcgcaa gtagaacaag gagattaaag
     9601 aagagagatt gtgagcctga cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag
     9661 cttctcatat ttgttgaata gtgtaactcg tgattcggag ttgacaagag caacaagaag
     9721 cgattttgaa gtatagcttc tcgtatttta caagatcgtg tcttattgcc gataggttgt
     9781 ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa gtcttttgtc actacttacc
     9841 gaacgaaact gaaggagcta tccaaaaagt atcattagta ttattctttg tgtttctagt
     9901 ggcaccacta gtacatttaa aaaagctatc gatcatggac taatcataca tgatcgatag
     9961 ctttttagat aatgtttgtt acgctatctg aaattgacta ttataaagac tggcataaag
    10021 cgttggtgat tggagtagtt cagaatgtgt tcctttttcg acgatatcgc cattcttcat
    10081 tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt
    10141 cctatctttt gtcacagctt ccattgcttg ctgaatcaat gcttctgttc gtgtatctac
    10201 gctagaagtt gcttcatcta aaataacgac aggaggattc gctagaatga ctcgagcaat
    10261 tgtcaataat tgttgctgtc cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc
    10321 atatccgtct ggcagtgttc gaataaagtg atcacattgt gcgatttcag ccgcttcgat
    10381 aatttgttct cttgtggcat ccattttccc gtaggcgata ttatcagcta ccgttccttc
    10441 aaacagccag gtattttgta aaaccatccc aaaagaacga cgtagttctc cacgtgaaag
    10501 cttggtaata tcaagaccgt cgaattcgat ctttccttta ttcagttcat aaaaacgcat
    10561 cagcagattg actaaagtcg tttttcctgc accagttgga ccaacgatcg ctaccgtttg
    10621 ttttggcttt actgaaaaat cgacttgatt catcaatatt ttatctgatg tgtaaccaaa
    10681 ctgtacattt ttgaaagcta tttttccttt cggttgttct aaaagtcttg catctggaag
    10741 gtctggaatt tcatctgttt catctaatat ctcaaatatc ctatcgattg ctgccattgc
    10801 ggattggatc gagttgatca cataagaagc ggtagaaatt ggttcagaaa tctgattgat
    10861 gtactgaaga taagcttgta agaagccgat cgtcacacca cctgatagaa caagcatagc
    10921 accgaatacc gcacttatga tgaaggcgag ttgattaatg aaacgaatgg ccggatagat
    10981 agcacagttc ataaattgag cacgtttaaa agcgttgtat tgctttttat ttacttcgtg
    11041 tatagcagct tctgcatgag cttgctgatt gaatgttttg accactagat taccagctaa
    11101 gatttcttcc attcgattat ttaaaacacc cagttcatac tgattctgtt cagaaatctg
    11161 tttgtttttg ttcgcaatct tagttgtaac aattgaactg ccaacgatca gcaataagac
    11221 gagcaaagta agcttgcttt cgatataaaa cagcatcaga ccagcagcag ctattgttac
    11281 gacagaagta aaaaattgat tgatacctgt caataacact tgagaaagtt gattgagacc
    11341 ggtagttgtc cgactaatga tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa
    11401 tgttttgaat ttttttgtga tttcttttcg caccctcaat gtgatctttt cgcttaacgc
    11461 tgccattgtc cgttcctgaa taaatgaagt gatgctgctt aagactgaac agccgatcag
    11521 caataagaca gggaacaaca atgcttgttt gatatcactg aaagaaagct gtccaatacc
    11581 tgtttttctt atttctgata agagatcatc gatcccgatt cccatgatca agggcatggc
    11641 tacgactaat aggttcccga ataaactgca tagaaacaat ccgtaaaaat atttatgttc
    11701 aggtttgatc attttccaaa aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc
    11761 catcgtatcc cttctttcta tattccttgt gattttgcaa actcctgata agtttggcaa
    11821 ttagacagta gttcttcatg agtaccttgt ccaacgattt ttccttcatc taaaacaatg
    11881 atgttatctg cttgttggat tgtactgagg cgttgagcaa caatcacgag agttttctcc
    11941 gacatgtgtt tatgaagggc tgctcttaat gctgcatctg ttttatagtc taaagcggag
    12001 aaactatcat cgaatacata gatctctgct ggtttgatca acgctcgagc aatacatatc
    12061 cgctgtttct ggccccctga ataattagaa ccgccttgag caacaaagct gtccaatccg
    12121 tcaggaaggg tgtccactag ttcttctaat tgagcgatct tgatcgcttt agacaactct
    12181 tctgttgagg catattcatt tcccatctgc aagttgctta aaattgtccc gctgaataag
    12241 aaagccttct gagggacata actgatgtgg gagcggatct gttcttgagt catttgagta
    12301 agaggaatac caccaagggt tacttttcct atcgtagggt cactaagtct caagagaagt
    12361 ttcgccaaac tactttttcc agatccggtc ccgccaacga tcgctgttgt ttttccttta
    12421 ggtattgtga aatcaatatc ttctagaact ggatcagaga tttcagtata tttaaatgtg
    12481 acatgttcaa aagcagctgc atttgtctga tcaaaggttg gttcagctac tgttttgatc
    12541 gtctcttcct caatttcgat ctctgcttgc agtatttctt ctatccgtcg taatgaggca
    12601 aacgatttag ggataatgac caataccata gctgcgatca tcaagtaagc aagtgtcagc
    12661 atagagtatt cgactacagc agtcacgaca ccgatttgta atgctccttc tgctacaaga
    12721 tttccgccaa accacaaaat agaagcataa acgattccca tgagcaaaaa agcaattgga
    12781 gtgacccaag caaatattcg gttcactttg atcatttgat tagcatagtc agcaaagctc
    12841 ttattcgttc gcttttcttc atatgattga ttatcaaagg cacggatcat attaatccca
    12901 gtaaagaatt ctctcaatgt gatggtgatc cgatccattt ttggctggat actcaaagat
    12961 aaaggtgccc ctcgtttcat cagtcgataa acaatccata cataaaaaac aacagagatc
    13021 aatgtaaccc atgttagtaa tagagaataa cgaaacatca tgagaatggt gaaaacgcaa
    13081 ataaccggtg ctggtaagat catttggcaa aataaaacaa tcatttgctg gacattatcg
    13141 acatcattcg tcattcttgt caaaagagat ccagaactaa aacgactgat atccttcatt
    13201 gacagctgtt ggaatttacg gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa
    13261 gccaatgctg aaaagtaact agaggcaact gcagcgattg ttcctagaaa cgctacaagc
    13321 agcatttcaa ctccaatttt ttttataact gaggcttcgc cacttgctat acctgtgtcg
    13381 atcagctttg ctactaattt tggtacacct aatgtaccgg cgatttctaa acagatcatc
    13441 acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga atgtttttaa gatagacata
    13501 ttattcctcc ttttttcctg atacttttcg attctatacc ttcaagtaac ttgaaggtca
    13561 atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg
    13621 gccaagtatc ggaactattt gatttgccaa aatcaacgat ccgctattgg gatgaacaag
    13681 gattaatccg ttcaagtaga caagaagaga atgattatcg attgtttgat atcgatgata
    13741 tttttatgct ttatgatatc gttttttata gaaaattaga tataccgatc aaacagatga
    13801 agaatttata tggaaaaaca ttgacggagt tatatgagac acttgatgaa acagaaagaa
    13861 gaatccataa agaactggtg gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa
    13921 aacagttgaa attgatgatc gatacaaacg aggaagaatt tcctgtagaa gagatccctt
    13981 ttgactgtat gatctcaacg gagtttgaag atattgtgga aatcaaaaaa tttctaccta
    14041 actattcaag ctttgggatg atgagtatgc cagcttccag ttcacagacg atgtatggat
    14101 tttttatcga ccctagtgaa gtacatttat ttactcaaga cgtgatttgg gagaaaaaag
    14161 atacggctgt ttatcgtcga ttcttattga aatccgagat gaaccatgcg gaacgtaata
    14221 atattttaga aatcagagaa agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg
    14281 gccagtatct attgacgaat acggatgaaa acaatatcag aactgaatat tatcatgcat
    14341 ggatagaaat gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg atggttgttt
    14401 tagttataaa gaaaaatttt ctggttcgat tacgggttta atgtggggga ttattagtcg
    14461 tggatagata gaattttagt tttttagagg aaatatgtct ttaaaaatgg aatggttgca
    14521 attcttagcg atatatacga tgtaattgtc ctcttaaaaa agtatggaaa aataattgta
    14581 ttgggtgtta tgtataattc atggtgaact gagactaatg agaagaaaaa ttcatattgt
    14641 tatgagtgag attgtatcag atgagaatga taaattaaat aggtgcataa aagcattaca
    14701 cttaaaatac attgttgctt acattattac aatatgttaa taaggcaaaa ataaatagat
    14761 ggattgaata aacatatttt ttaatcaaat agtccctact gtgactttcc tca
//
LOCUS       DAZUMI010000071        14725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000071 DAZUMI010000000
VERSION     DAZUMI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.072"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..995
                     /locus_tag="ACG81C_001866"
     CDS             99..995
                     /locus_tag="ACG81C_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5065019.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1145..2086)
                     /locus_tag="ACG81C_001867"
     CDS             complement(1145..2086)
                     /locus_tag="ACG81C_001867"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5065020.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2212..3333
                     /locus_tag="ACG81C_001868"
     CDS             2212..3333
                     /locus_tag="ACG81C_001868"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5065021.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3591..5093
                     /gene="eat(A)"
                     /locus_tag="ACG81C_001869"
     CDS             3591..5093
                     /gene="eat(A)"
                     /locus_tag="ACG81C_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5065022.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5160..5687
                     /locus_tag="ACG81C_001870"
     CDS             5160..5687
                     /locus_tag="ACG81C_001870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5065023.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5898..6959
                     /locus_tag="ACG81C_001871"
     CDS             5898..6959
                     /locus_tag="ACG81C_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5065024.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6962..7858
                     /gene="murQ"
                     /locus_tag="ACG81C_001872"
     CDS             6962..7858
                     /gene="murQ"
                     /locus_tag="ACG81C_001872"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5065025.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7872..9854
                     /locus_tag="ACG81C_001873"
     CDS             7872..9854
                     /locus_tag="ACG81C_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5065026.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9919..10365)
                     /gene="nrdI"
                     /locus_tag="ACG81C_001874"
     CDS             complement(9919..10365)
                     /gene="nrdI"
                     /locus_tag="ACG81C_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5065027.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10576..12000
                     /locus_tag="ACG81C_001875"
     CDS             10576..12000
                     /locus_tag="ACG81C_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5065028.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12181..12954)
                     /locus_tag="ACG81C_001876"
     CDS             complement(12181..12954)
                     /locus_tag="ACG81C_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5065029.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12951..13856)
                     /locus_tag="ACG81C_001877"
     CDS             complement(12951..13856)
                     /locus_tag="ACG81C_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065030.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14010..14330)
                     /locus_tag="ACG81C_001878"
     CDS             complement(14010..14330)
                     /locus_tag="ACG81C_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5065031.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4720 a         2616 c         3107 g         4282 t
ORIGIN      
        1 ttaaattttc atacaaatat aggaaacgtt ttactggctt tttactattt aaacaggcta
       61 tcataggaaa aagtgacaga gaaagtgagg cggaaaagat gaacaaaaat gacgtactgc
      121 tagttgcaaa aaataaatca ctttattttt tgccttccat ccgctcttta gatgtttggt
      181 ccggaaaaga tttactacag tcggaattca ttaaatcagc tagacaacag ttggcaaatt
      241 atcagctgat cgttttttta gattatggat ttgatatgga gatggctcga cgcgtgcgcc
      301 catttaccaa tgcaaaaatt gtgttatttt tttggaatca cttcaaagca gaacacgagg
      361 cgatgctgaa gcaggcacaa gatgaaccgg caatcgatga gatttatcat tttgattttt
      421 tggaagcaaa agaacttggg ctgaaacata atagttcatt ttattcgaaa catatgaatt
      481 tttcttctag cgaaccaaag acggatctat tttttggtgc aacgaataat gggagaaaag
      541 aacgagcaga aagctataag caggaattta taaaaagaga tgttacgacc aactatttca
      601 ttcttccaca tcgagggaat gaacaacctg gatatcttac ttatgaagag tacctgaaaa
      661 aaacagggga atcgcgcggg attctggaac tgttgcggga gggtcagcaa ggagtgactt
      721 tgcggacatt tgaatccatg tattttcaaa agaaactagt gacggataat caagcgattg
      781 ctcattatca tttttatcgt cctgaaaata tttttttgct tcgagaacgt gatttagatg
      841 aattaccgga atttctgcat acaccattta agccaatcct atcagagcta ttggaatttt
      901 atgatgcaga aaattgggca aaacgattta tcactaacga ttccactatt tatgagcaat
      961 acgaatatga cctctcctct gttgaaaaca aataaaaaac ctacattttg aagagattat
     1021 gtactgaaac aaacaagcac aattcttatg gcatcatgag agttgtgttt tctttttgat
     1081 cttttattgg gcttaaaaaa agagaaccag ctgctgattc agctgattct catcagagag
     1141 ccttttattc tgcgccaagg attccttctt ttgcatggac taattgggtc aaaaaggcac
     1201 aatatggtgt agcaatatca tatttttgtc cttttcgcca aatggcacca ttgatgtagt
     1261 cgatttcagt caaacgatgg tttttgatca ggtcttggta catagatggg taatgcaacc
     1321 cgatgttgtc aggatcataa gtagaagcaa tatgttggta cacttcttct tgatctagta
     1381 tgattccttc ttttgctgcg atagaagcaa attcagatac gattgttcga accatgctct
     1441 ctgccgcttt ttgcgcgcct agttcagcaa tattacaatc caaaatcgtg cataaaccat
     1501 tcaatgttcc atttacacaa gcttttcgcc aaatggaata acgaacgtta tggctataga
     1561 tcggatttaa atttgcttct tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt
     1621 ctggttccaa gttctctagt tcgatttctc catcacctaa caatgtgact tttccgggac
     1681 cagctagacc agctgtccac atcgtgatcc caaataagat atttttcttt ggcacatatt
     1741 tttccagtac atcttcatga cctaatccat ttagtaaaca caaaacataa gtattgtcag
     1801 taataatgga ttggatcgaa caaaacatat catctaattg attggctttt gttaatgcta
     1861 cgatcaagtc cacgtgttcg ttgctttcga tgatttcttc tggtgaatag attggcagtt
     1921 ttgcgaccac ttctttaccg ttaaagtcag caatcaaccc atttgtacga atggcttcaa
     1981 tgtgagcagg ccaacgatcg atcaagagga cctcatttcc gctttgatgt agcatcaatc
     2041 cgattcggct gcccattgct ccggccccag ctattgcgat tttcatgttt tttctctcct
     2101 ttattcaatg agttcccttt tagtatatag aaaaatggaa gaaaatagca ttgttttgtt
     2161 tgaaaaaaat gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt
     2221 ttggcggcaa ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt
     2281 ctaaatggct gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga
     2341 gaaaatactg cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg
     2401 atggatctct tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa
     2461 aacaccgcag tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg
     2521 gatcaaacta tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg
     2581 cttcaagaag tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt
     2641 ctaggattac tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta
     2701 ttatctacta aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg
     2761 attatcgctg cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt
     2821 ggaagacaaa aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag
     2881 attgctcagc aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga
     2941 gcagcaggcg gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc
     3001 gatttagtca gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt
     3061 acaggagaag ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca
     3121 gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag
     3181 ctagaaggac tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt
     3241 ttagaagaag caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca
     3301 cttagcagat ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat
     3361 cgtaatttca aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga
     3421 tgaaagctgc attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg
     3481 attggcttgg ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct
     3541 ttagccagtt ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa
     3601 tcgaaataaa aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag
     3661 caaatctaaa ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa
     3721 agacaacttt actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc
     3781 agcaagaatt tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg
     3841 tgttaaatga tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc
     3901 aaacagatcc tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa
     3961 aagtcctact ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa
     4021 cgaatcattt ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac
     4081 aaggcttcat cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt
     4141 tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac
     4201 agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca
     4261 gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata
     4321 aaacaaagaa acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag
     4381 tgggtgctga tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga
     4441 cccagatcag cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga
     4501 tgaatagcca agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt
     4561 atgaaaatct gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga
     4621 tttcaggtcc taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca
     4681 acggcaaggt tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc
     4741 aaaattatga agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact
     4801 accaagcatt tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca
     4861 agatcgagca gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac
     4921 agccagctga actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag
     4981 aacaattaga acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc
     5041 aaaccttcct ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt
     5101 aatctcaaga acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca
     5161 tgctttatgt aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac
     5221 gggcagatgt gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg
     5281 tagtagattt gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag
     5341 atacagcaca agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg
     5401 tagaaatgga ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag
     5461 cacgtttagc gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac
     5521 ggatcgttcc tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt
     5581 gccataatgc cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt
     5641 tctttacatt catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg
     5701 ttatttaaat aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat
     5761 gacaattctt ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa
     5821 aagtattgaa attttgtttc tttagatata tactctagtc atagaaatga agacggatac
     5881 agaaaaagag ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag
     5941 agaagaaaca gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca
     6001 tgcatatacc agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg
     6061 aagcgaaaaa gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag
     6121 cgggtttttc attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa
     6181 tggattatca tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt
     6241 tgaatgctag cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt
     6301 tctcacagat tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag
     6361 aatggtatca gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg
     6421 taccaggaga tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac
     6481 atcgagggat ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg
     6541 tttacatcgg agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa
     6601 aagaacagac agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat
     6661 tagggaagca tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac
     6721 gttttcgaga gattccgcct atcccagcaa gaaatacagc tacgcgtatg aaagggtcta
     6781 ttactttaga taacgaaaag tatttgcgct atatgggtga gatccaatta accaaatatg
     6841 atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa gagctaccat
     6901 tgatcaacca aatcaaagct ggtatgaact atcaatttat aagaaaagaa ggaagataaa
     6961 aatgagcaaa atcaatttag agaacctaac aacggaacgc agaaaccaag aaacttttgg
     7021 attagatgag atgagtattt ctcaagccgt tcaactaatg aacaaagaag atcagaaagt
     7081 ggccgctgcg gttgaaaaag aactgacaag tatcgaacca gtgattgcta acacgattga
     7141 gtcctttaaa aaaggtggac gtctgattta tatgggagcc ggaacaagtg gaagactagg
     7201 tgtgttagat gctgctgaat gcgtaccgac attcggtgta gaaccggaaa tggtcatagg
     7261 tctaattgct ggtggagaag aagcaatgac tgtagctgtc gaaggggccg aagattctta
     7321 tgagctaggg caagaagatc taatcaagct gaatttgaca aaaaatgata tagttattgg
     7381 tatcgcagct agcggtcgta caccatatgt gatcggtgga ttagattacg caaaatcgat
     7441 cggtgctgtc acaggaacga tttcttgcaa taaacaagcc gaaatcagta aacacgcgga
     7501 tttcccaatc gaagtcgatg caggtccaga atttttgaca ggctctactc gattgaagtc
     7561 aggaacagca caaaaactta tcttgaatat gatttctact atcagtatga ttggtatagg
     7621 taaagtattc aataacttga tggtcgatgt caaaccaaca aatgaaaaac taatcgaacg
     7681 ctcaaaacga atcatcatgc aagcgacaga agctgattac gaaacagctg aaaagtactt
     7741 tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg attttgacaa atagtgataa
     7801 agaggaagct caagcaaaat tagctgaagc aaacggattt gtaaaaggga ccttatgagg
     7861 gggaaaagac catggcaaaa aacgaactga ctttggaaga actggcaaaa caaatttata
     7921 aaggggcagg cggcatgggc aatgtagaaa gcattgtcca ctgcatgact cgtgtgcgta
     7981 tgaagatcat caatgatgat cttgtaaatg tctctgaatt aaaagcgatt ccaggagtgc
     8041 ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg acctgggaaa gtgaacaaag
     8101 tagcacagca aatggttgat ttggcaggcg tgaaattagg agaaaaactt tcttctgtaa
     8161 gtacagctac agcaaattca tcagcatctg gaaaagaccg tgtaaacgaa cgggcacaag
     8221 aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa aatcaaatcg gtattaaaag
     8281 acatttctaa tatctttgtg ccaatgattc ctgcatttgt cggtactggg atcgtggcag
     8341 ggattgccgc agttttagca aacttagtca cagccggaac acttgatgca gcaacatggc
     8401 aacaatatat cgatgtgatg aacatcttga aaaacgggat gtttagctac ctagttatct
     8461 atacaggtat caatgctgca caagtttttg gtgcaacacc aactcttggt ggagttatcg
     8521 gggccgttgt gatgctgaca gggatgaatc cggaagcacc actgaaaaac ttatttactg
     8581 gtgaagctct tgcagctgga caaggtggga tcctaggtgt catcttcgct gtttggctat
     8641 tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga tgcggtagat atcatcgtta
     8701 caccaactct ttcattgatt gccatcggtc tgattgaaat tttcttcatc atgcctttag
     8761 caggtttcat ttctgatggc atggtcggtg ggatcaactg ggtacttggc gttggtggag
     8821 catttgccgg cttcgttcta ggcacattat tcttgccgat ggttatgttc ggactgcatc
     8881 aaattttgac tccaatccat gtgcaaatga ttgatgaaac tggtcgtacg ctattgcttc
     8941 caattcttgc gatggctggt gcaggacaag tgggtgcagc attggcatta tggattcgct
     9001 gcaaaaaaga taaagaatta acagaaatga tcaaaggagc tttaccagtc gggatcctag
     9061 gaatcggcga accattgatt tacggagtaa cattacctct aggacgccca tttattactg
     9121 cttgtatcgg tggaggtatc ggcggggcag tgatcggaat gatcggaaat gtcggggcta
     9181 tcgctattgg accttcagga gccgctttga tcccattgat ctctgacggt aaatggttag
     9241 gctacatcct tggattattg gcagcttatg ctggtggctt cgttgctaca ttcttcttcg
     9301 gtattcctaa agaccgttta gaaaaagaag aattagcgac tgaaacagcc acagtaaatg
     9361 aagtgcctga aaaagtagta cctcaaactt cagctgaaag tgtgactatc ctctcatcag
     9421 ttgctgacgg aacagttgtt tctttagata aagcaagtga tccagttttt gcacaaaaga
     9481 tgatgggtga aggctacttt gtagaaccag aaaacggaca tatctattct ccagttactg
     9541 gggtagtcag ctctgtcttc ccaacgaaac acgctattgg gatcacaacg ccaaatggtt
     9601 tagaaatctt gcttcatatg ggaatcaaca cagttgactt aggcggtacg ccatttgatg
     9661 tgaaagtaac agaagggcag caagtgacac ctgatacgct tgtagcagaa gctgacttag
     9721 aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt cttggtaacc aatatggatc
     9781 gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa agcaaaagca cctgtgatgg
     9841 atgtcaaagc ttaatatgat agaaacaata gaaacgaaag aagaaactga aatctatttt
     9901 tcagcttctt cttttttatt atttattgtt ttctgctaaa atctgataaa ttcgtgtcac
     9961 atcagatggt gttccccgta attcataatc tgccaagaaa ggaaagccaa atgtttgtgc
    10021 atattgtttg gctgttagac aatactgatt attgaagttt ttatttccgc taccgactac
    10081 accaagacaa agtttatgat tgtcttcatg ttccagatat tcacgcatcg tttcggtcaa
    10141 gatttctgta tcaccgttat ctaaaccatt tccgccgtct aaataagtag gaacgaaagt
    10201 aaaaaatggt tctgtttctt tcgtaaaatc actattttca tggatttctt ttaaagtaac
    10261 ttcaggattg acaggatctt ctgcatgcat tttttcagca tattctgcta gatgtttggc
    10321 aaaggcacgc gtatttcctg aaatcgatat atataaaatg ttcatggatc atcctccaat
    10381 tctttccttt tagtatagca tcttttttaa gttttttggt ggtgtaattt ggaaagggac
    10441 taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa ttatatttct attaaagttt
    10501 ttattaatta agtaaaaata aaaaaggttt gttatacttt ttgataaaag cggttttatt
    10561 ttggaggatg cttacatgga tacagagcaa cagaaaaaag gaattggttt aattccactt
    10621 gctgccttag taattggttc agcgattgga ggtggcgtat ttggtattat gacggacatc
    10681 tccggatcgg ctggcggccc agctattttc agttgggtac tcgtaggaac agcaatgttg
    10741 atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc cagatttaaa aggcgggatc
    10801 tttagttacg cagaagaagg ttttggccgt ttcgctggtt ttatcagcgg ttggggttac
    10861 tggctcacat gctggttagg caacgtagct tttgcaacat tgttgatgag cgcattagga
    10921 tatttcttcc cagtatttga aggtgggcaa aatctggcat cgatcgtgat gagtacagtc
    10981 tttttatggg gatatgcatg gctagtgaat cggggagtcg aaaatgctag tttcgtcaat
    11041 gctatcgtca cattctgtaa attagtgcca ttatttttat ttattgtagt agctatcagc
    11101 acgttttcga tccaacaatt cattgataac ttttctagcg acattatatt gaatgcgaat
    11161 ggacagccga tcccgttttc cagtcaagta ttgagttgca tcatggtgat gctttgggta
    11221 tttgttggta ttgaaggagc ctctgtcgta ggttcacgag caaaaaaaca atcagatgtt
    11281 gggaaagcaa cgattctagg gatattcggc ttgattatta tttacgtctt ggtttccatg
    11341 cttccatatg ggacaatgac gatgaatcag ctgcaaacga tcaaggcgca gcctgctatg
    11401 ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag tcgtgatcaa cggcggtttg
    11461 atcatttctt tagttggtgt ttggctctca tggacgatct taccgattga aacaatggga
    11521 aacatggctg aagacggact tttgcctaaa gcatggggaa aactcaacaa aaatggggca
    11581 ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg tctttttact ttcattatta
    11641 ttcactgaca gtgcttataa ctttgcttac acacttggta cagcagctat tttcttcacg
    11701 tggcttttct taggattgta ccaaatgaaa ctttcttatc agcggaaaga atgggtccaa
    11761 tttttagtag ggcttttagc aagtgccttt caagtatggg ctatgctagt cgtagccttt
    11821 aatgaagtaa tgcttgttct tgttcttttc ttgccaggca tttattttta cctcaaagca
    11881 cgtaaggagc aaaacgagcc tgtacaacat atccacttag ataagatttt acttataggt
    11941 gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca ctgggatgct tcacgtatag
    12001 aagtataaaa gaagtataaa gagaggttgt gagcctgtcg tttattcaaa aacatactag
    12061 gaaaggagct gtgacacaaa tgttgtcaca gctccttttt cagaataaac ggtgtttaag
    12121 agacagccaa tcggcaataa ggctgaacgt ctctttcaca gcctcttttt tgatgagtca
    12181 tcatacattt tttcgcttaa atacgagata tcctaatgct aagaacaaaa cgatgtaagc
    12241 taaattaccg aatagcattt gattagtaga aagatgagtc aattgcttca tgatctcttc
    12301 gtttccaatt tggttttgca gattcaacat actgatcgga ttccatttga tccactccca
    12361 tttttggatg agtgcaataa ggataccaga tatgattgac gaagcaaaat aaaagacgat
    12421 tcctgctgag atggatgcgc cggaactatt gataaagcaa gcaatcatca acactaaact
    12481 taaaatcagc cataaaccga tataactacc caaagtataa gtgaacaagc tttggatcag
    12541 tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa gggaaaagaa tcagtttcat
    12601 caacacagtg acaataatag ctaaaaggta taaataaaca gaataaataa ctaatgtgat
    12661 ccatttgctt actaatattt ctcctcgaga atactttcga taaaggagat ttttcaatgt
    12721 accatattgt gcttccatcg aaatgatcgt gcttgcagct gcaatcatga taaagacaat
    12781 ccaagatgtg gcactaaaaa gctgagcagt catttcaact ggatcaatga tcgtcgtgta
    12841 ttttttggct agaagtgctg ttcctatgag caaaacaact aatagaacag ataaaacagc
    12901 agttgacttt ttcttgatca atttaaataa ttcttgtttt acaagcaatg tcatttttct
    12961 tcttccccct ttactgaatc cagaatttcc aatagagaag attccaaatc attcgtcaga
    13021 tgctggacgt ctgtcacatg gatattttgt gcagacaata gctggatcac ggttcctaat
    13081 aattcttcgt tttcttttaa aagcttgatt ttatcaggat cttccaaagt atatccagct
    13141 tctgtcaaga tcgttcgagc agctagatca tttgttgtag agataacgat gaaatcttgc
    13201 gctgtttggt tcaattcagc cattgtacat tgtttgatga tctttccttg attgatcagg
    13261 atcagctcat cgaccatttt ttcaagctcg ctgagtaagt gactggagat caagaaagag
    13321 gtgccctgag tagccaagtc ggtaatgatt tttcgcacat ctcttgttgc ttgtggatcc
    13381 aaaccgttca tcggctcatc taaaataacg atagacggat ggttcagtaa ggcaagagca
    13441 attcccaatt tttgtttcat tcccaacgag taggatttcg cttttttgtg aatgtacttt
    13501 tccatcttga gctgattcac gatagtgtcg atagcagata catcgttggt ttcagagaat
    13561 aacttcagat gatcatagcc tgttaagaat ggatagatac ccgggtattc gatcaatgct
    13621 cctacttgtt ccaacccttt atgactagag ggagaaatct cattttcacc aatacagatt
    13681 gttccatctg agtaatggat cagtcctaaa atagctttca tgatcgttgt ttttccagcg
    13741 ccattaggac ctactaatcc aacgattttg ccaggggata agctaaaact gacatcgctg
    13801 agggcttgtt tgtgaccaaa gttcttggat aattcttgga ccgaaagact tttcaaaaat
    13861 attcactcct tttctataga tagtacaagc atacacctga tgagtggaga agtcgctagg
    13921 tcattggact tatttcttat tttttcataa aaaaattgaa tattaaataa aaaggcttat
    13981 tttttctgat cagtacttat tccaagtgtc tagtggccgc tagtaatctt gatgccaatg
    14041 atactgatga gtagcatccc cacaaagaac caagtggaaa gattcagctt atcatgaaac
    14101 aagaccacac caattatgac cgaaccaact gcgccaatcc ctgtccagat aggataagag
    14161 agactcatcg gcaaactttt gattgccttt gacaaaaagt aaaaactagc aatcatgcca
    14221 atcactgtgt aaaaagaata attgatttta gaaaaaccat cactccattt catcatcgtt
    14281 gaccagaata cttccaaaat tcctgctatg attaattcca tccatgccat acttacattc
    14341 ctccttaact ataaaaaaat acacaaaaag aggaaaatca tcccttcaaa gacctaatgg
    14401 aatgatcatc ctctttatcc tacccggtgg cagattgtat atctttttct gtagtatagc
    14461 acacctatcc agatatcggc aatctcatac tttttagtaa agtgtaccaa gcactgacgg
    14521 atatatcgtt agcggaaaag aagatgaatg ctttgcaatt tcatagaaag aagttgttat
    14581 ttattaatca tttaaaaaaa acagtaacta gaccttattt ctatatatta tcgggcaata
    14641 aattaaaaaa agctttacct tacaataagt aagataattt ttgtgataaa aaaataacga
    14701 aaaatattta aaaatagtgt ctatt
//
LOCUS       DAZUMI010000072        14652 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000072 DAZUMI010000000
VERSION     DAZUMI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.073"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..941
                     /locus_tag="ACG81C_001879"
     CDS             129..941
                     /locus_tag="ACG81C_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5065032.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            938..1738
                     /locus_tag="ACG81C_001880"
     CDS             938..1738
                     /locus_tag="ACG81C_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5065033.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1899..3167
                     /gene="rseP"
                     /locus_tag="ACG81C_001881"
     CDS             1899..3167
                     /gene="rseP"
                     /locus_tag="ACG81C_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5065034.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3235..4944
                     /locus_tag="ACG81C_001882"
     CDS             3235..4944
                     /locus_tag="ACG81C_001882"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5065035.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5156..9514
                     /locus_tag="ACG81C_001883"
     CDS             5156..9514
                     /locus_tag="ACG81C_001883"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5065036.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9659..10132
                     /gene="rimP"
                     /locus_tag="ACG81C_001884"
     CDS             9659..10132
                     /gene="rimP"
                     /locus_tag="ACG81C_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5065037.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10155..11330
                     /gene="nusA"
                     /locus_tag="ACG81C_001885"
     CDS             10155..11330
                     /gene="nusA"
                     /locus_tag="ACG81C_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5065038.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11352..11645
                     /locus_tag="ACG81C_001886"
     CDS             11352..11645
                     /locus_tag="ACG81C_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5065039.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11642..11953
                     /locus_tag="ACG81C_001887"
     CDS             11642..11953
                     /locus_tag="ACG81C_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5065040.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11966..14272
                     /gene="infB"
                     /locus_tag="ACG81C_001888"
     CDS             11966..14272
                     /gene="infB"
                     /locus_tag="ACG81C_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5065041.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14299..14646
                     /gene="rbfA"
                     /locus_tag="ACG81C_001889"
     CDS             14299..14646
                     /gene="rbfA"
                     /locus_tag="ACG81C_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5065042.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         5016 a         2582 c         3250 g         3804 t
ORIGIN      
        1 taaaaattaa aaaaagaaaa aattattcag tttttaaata aaaaaacaat gatttcatag
       61 gaaaaacagt tagatattgc tataatactt aagatagtgg gaatctttta agtataggag
      121 gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata tattcaagaa aaaagcgaat
      181 ttcaatttaa taccgatgga ccaattcctc agcacattgc catcatcatg gacggcaatg
      241 gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg ccataaagaa ggcatggaaa
      301 cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt gaaagtgttg acgctttatg
      361 ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt aagttttctc atgcaactac
      421 ctgtcgactt ctttgatacg ttcgtaccag aattgattaa agaaaatgtc aaagttcatg
      481 tcatgggtta tgaaaacgta ttgccagaac atacgcaaga tgctgtaaga cgtgcaattg
      541 agcaaacaaa aaacaatact ggaatggtat tgaattttgc tttgaattat ggaagcagag
      601 cagagatcgt gactgctgtc caagaaattg ccgaagaagt agcaaaagga gaaatacacg
      661 cagaagaaat cgatgatgaa ttgattgcta agcatttaat gacaggtttc ttgcctaaag
      721 agttacaaga tcctgagctg atgatccgta caagtggaga agagcgaatt agtaattttc
      781 tgttatggca gatcgcatat agtgagctgt attttacaaa agcgctttgg cctgattttg
      841 atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa tcgagaccgt cgattcggcg
      901 gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg aaacaacgtg tggtcacagc
      961 agccgtggcg ctagtcttgt ttattccgat tatatggatc ggaggcatag cggttgagct
     1021 tacagcagca atcttagcag taataggagt ttacgagctg tttcggatga aaggattgac
     1081 actcttaagt tttgaaggag ttttatccgc cgttggtgca gtgttacttg ttttgccaaa
     1141 agaaagatgg tttttctttc ttccagagaa tgtaagtaca tttatgttat tttatcttat
     1201 agttatgatt cttttaggaa catcagttat ttctaaaaat acgtatacga ttgatgaagc
     1261 aggttttcct gtgcttgtca gcttgtatgt gggaataggt ttccagaatt ttgtcaatgc
     1321 acgaacagaa agcttggctg ttttgatatt tggtctattt attgtttggg caactgatat
     1381 cggagcatat atggttggtc gacgttatgg tcagcataag ttaagtcctg atatttcgcc
     1441 caacaaaacg attgaaggtg cgctcggtgg tattggtagt gctgttgtag tcgcatttct
     1501 ttatttattg ctatacccag caaaagaact atttaatcat ggtatgtttg tcatgcttct
     1561 gatgacgatt attttttcag cagtcggaca atttggtgat ttggtcgaat ctgcaatcaa
     1621 acgtcattat gatgtgaaag actcgggaaa tatcttacca ggccatggtg gaatcttaga
     1681 ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat ttatttggca ttatttagta
     1741 agtatacctg cgtttctttg tgaatttcat aaaagacgca ggtttttatt tttcattata
     1801 ttgatactat tggtgtcttt ggtatactat ggtaaaatta tagtgtttta tcgttgtttt
     1861 ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat gaaaacgatt ctgacattta
     1921 tcatcgtttt tggtatatta gtgattgttc atgagtttgg tcatttcttc tttgcgaaac
     1981 gatcaggaat cctcgttcgc gaatttgcta tcggtatggg tccgaaaatt tatgggcatc
     2041 aagcaaaaga cgggacaaca tatacgcttc gcctattacc tattggcggt tatgttcgaa
     2101 tggcaggcaa tggggatgat gaaacagaaa tggcaccagg aatgcctctt tctcttttac
     2161 taaattctga cggcatcgta gagaagataa atttaagtaa gaaaatccaa ttgacaaatg
     2221 ctatcccgat ggaattgagc cgttatgatt tagaagatga gctgactatc actggttatg
     2281 tgaatggaga cgaaacagaa gtagtaacct accctgttga tcacgatgcg acgattattg
     2341 aaaacgacgg aacagaaatc agaattgcgc caaaagacgt acagttccaa tcggctaaat
     2401 tgtggcagcg tatgctgacc aactttgctg gacccatgaa taactttatt ttggcaatcc
     2461 tgttattcat cattctggca tttatgcaag gtggtgttca agtgacgaac actaatcgcg
     2521 taggagagat catgcctaat ggagctgcgg cagaagcagg cttaaaagaa aatgatgagg
     2581 tagtcagtgt ggacggaaaa gaaatccatt catggaatga tctgacaaca gtcattacga
     2641 aaaatccgga taaaacatta gactttaaga tcgaacgaga aggtcaggtg caatctgtcg
     2701 acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt tggccaactt ggtataaaag
     2761 ccccaatgaa tacaggtttt atggataaga tcattggagg gacgcgtcaa gctttcagcg
     2821 gttcattgga aattttcaaa gccttaggat cactatttac aggtttcagt ttagataaat
     2881 taggcggacc tgtcatgatg taccaattgt cttcagaagc agcaaatcaa ggtgtaacaa
     2941 ctgttattag tttgatggcg cttctttcaa tgaatctcgg aatcgtcaat ctgcttccga
     3001 ttcctgcctt agatggcggg aaattagtct tgaatatttt tgaagggatt cgcgggaaac
     3061 cattaagtca agaaaaagaa ggaatattga cattagctgg ctttggattt ttaatgttgt
     3121 tgatggtgct agttacatgg aacgatattc aacgattctt tttctagaaa aagttgacaa
     3181 atagggtata ataacaaacg ataactattt tgaggaaata aaggagtata tagaatgaga
     3241 caatcaaaaa tgttgattcc aacattacgc gaagttccaa atgatgcaga agtattaagc
     3301 catcaaatct tgctaagagc tggatatatc cgccaagtat cagcgggtat ttattcatat
     3361 ctgccattag caaatcgtgt attggaaaaa ctgaaacgca ttatgcggga agaatttgaa
     3421 aaaatcggag cggtagaaat gctgatgcct gccatcttgc cagcagaact ttgggaagaa
     3481 tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt tcaaagaccg caatgatcga
     3541 aaaatgatcc ttggaccaac tcacgaagaa acttttactg atttgatgcg taatgaaatc
     3601 aattcatata agaaattacc tttaaatctt tatcaaatcc aacctaaata tcgtgacgaa
     3661 aaacgacctc gcttcggttt gcttcgcgga agagagttta tcatgaaaga tgcgtattct
     3721 ttccatgcaa cggaagaaag tttggacgag acatataaag attacgaaaa agcctacacc
     3781 gaagtattca agcgttgtgg cttagaattt cgctcgatta tcggagacgg tggcgcaatg
     3841 ggcgggaaag actcaaaaga attcatggcg atctcagaaa tcggggagga tacgatctgt
     3901 tattcgactg aaagtgatta tgctgcaaac ttggagatgg ctacaagcta ctatgtatca
     3961 aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag ctacacctaa cgtaaaatca
     4021 attgacgaag tagcagattt cttttctgtt gaacctcaaa aaatcatcaa gtctgtattg
     4081 ttcattgcgg atgaacaacc tgtccttgtt ttggtacgcg gagaccatga agtcaatgac
     4141 gtaaaactga aaaacttttt atctgctgac tttttagaag aagcaacaga agaagaagca
     4201 caaaaatatc taggtgctga gtttggttct gtcggaccag tcggtgtttc agatgacgta
     4261 aaagtatatg cagaccgtta tgtccaagat ttagcgaatg ccattactgg agcgaacgaa
     4321 acaggattcc actataaaaa tgtgaatcca gaacgcgatt tcactgtact ttcttatgaa
     4381 gatcttcgtt ttgtacaaga aggagatcct tcaccagaca acaatggtgt tcttgccttt
     4441 actcgaggaa tcgaaatcgg acatatcttc aaaatcggca ctcgttacag tgaatctatg
     4501 ggagcaactg tactagacga aaatggacga gaaaaatttg tgattatggg ctgttatggt
     4561 atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac attcagatga acaaggaatc
     4621 aactggccaa aaggaattgc gccatttgat ttgcatatcg tccaaatgaa tctaaaagac
     4681 gaataccaaa caaacctgac gaatgaacta gaagaagcga tgacaaaagc tggctatgaa
     4741 gtactggttg atgacagaaa tgaacgcgca ggagtgaaat tcgcggattc tgatttgatt
     4801 ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg atggaatcgt agaagtgaaa
     4861 atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag aattggtgaa tactttacca
     4921 atcctcttga accaaggcga ataataagcg ttaaaacaca gaaaataaaa atagctgaat
     4981 cagctgtttt tgacaatctg atgattcgct tttaaaattg gaactgcgat ttgaatcgca
     5041 gttccaattt tttaatgtaa aactcagaaa aaacgagtat actaaaaggt actgaaaaag
     5101 aagaggataa gatgcgatca attgcattgt ttctcatcag aaagggcgta tatctttgtc
     5161 agaaaaacga gatttatttg aaaaactgtt ggaacaaatc caactagagg aagcagaaaa
     5221 aaatcatcca ctgcttactg ctggtgaaat ggatagtgta gtggtccatc gtaaatcgcg
     5281 tttatgggaa tttacgctgc atttttccaa aattttgcct attatgctat atcggtcatt
     5341 gactcagcat ctgactatcg cttttaaaga tattgcacaa gtcaaactga atatcttagc
     5401 agatgaccag acattcgatg aacagctctt gcaagattac tgggcccaag ctttggaaaa
     5461 tcagcaatgc gatacaccac tagcacagca agtactgaaa acgcaggtac ctgtgataaa
     5521 agatcgtaaa gtcatcttgc caatcgacag tacaggagct atttcttatt tgaagcaaca
     5581 atacttgcca ttagtagaag agttgttcgt cagttacgga ttcccaaaat ttcgtatcga
     5641 accagaagtg gatgagcagc aagcggaacg agtattgaaa ctttttgaag aacgcaaaca
     5701 agaacaggca gaagccttta tgaagcaagc agctgaaagt ctagttgtcc acgaacaaaa
     5761 gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga cccatccaca tccagctagg
     5821 acgaaatatt ccagccgatg aaccaacgac accaatgatc agtatcgttg aagaagaacg
     5881 aagagtcaca cttgaggggt acgtattcga caaagaagtc agagaactac gttcaaaacg
     5941 caagatcttg actttgaaaa tcacagatta tacttcatca tttatcgtca aaaaattctc
     6001 gaacggcgaa aaagatgaac aagtatttga tgcgatcagt gtcggtagct ggttaaaggt
     6061 acgaggaagt attcaagaag atacgtttgt ccgtgatttg gtcatgaatg cgcaagatat
     6121 catagaagtc aaacatacac ctcgcaaaga ttatgctcct gaaggggaaa aacgagtaga
     6181 gcttcatgtt catagcaata tgagtacgat ggatgcaaca aacagcatct ctgatcttgt
     6241 ggcacaagca gggaaatggg gacaccgagc tatcgccatt acagaccacg gtggtgcgca
     6301 agcctttccg gaagcacata gtgctggaaa aaaagcaggt gtgaaaatct tatacggcgt
     6361 ggaagcgaat gtcgtagatg atggtgtccc aattgcttat aatgatgcac atgaagcatt
     6421 gagtgaagcc acatatgtcg tatttgacgt ggaaacgaca ggattgtctg cagtatatga
     6481 cacgatcatc gagctagccg cagtcaaaat gtataaagga aatgtgatcg aaagctttga
     6541 tgaatttatc gatccaggac atcctttgtc acgaacaacg gtagatctga ctggtatcac
     6601 ggatggaatg gttcgaggat caaagtcgga agaagaagta ttgcggatgt tccttgaatt
     6661 ttcaaaagat acaattttgg tagcccacaa cgcggctttt gatatggggt tcttgaatac
     6721 tagctacgca aggtatggta ttcctgaagc ggccaatcca gtcattgata cgttggaatt
     6781 agctagatat ctgtatcctc aattcaaacg tttcggatta ggtgtgttat ctaaaaaatt
     6841 tggtgtcagc ttggaacagc atcaccgagc aatctatgat gctgaagcaa ctggtcactt
     6901 agcatggatc tttgtgaaag aggcaatgga caatcataac atgctttatc atgatcagct
     6961 aaatgagcat atcggagaag gagattctta caaaagggct cgacctttcc atgtaacgat
     7021 tttagcaaaa aatcaggcag gactgaaaga tttgttcaaa ctgatttcaa tgtcgaatgt
     7081 cgaatacttc gaacgcgtcc cacgaatacc acgctcccaa ttgaaaaaga tgcgtgaaaa
     7141 tctattgatc ggctcagctt gtgataaggg agaaatattc gaagccatga tgcaaaaagg
     7201 ggtagaagag gcaagaaacc gagcaaaatt ctacgattat attgaagtta tgccaaaagc
     7261 ggtctatgcg cctttgatcg agcaagaatt ggtaaaaaat gaacatgatt tggaagaaat
     7321 tatccaaaat ttggtagaaa tcggaaaaag tttggataaa atcgttgttg caacaggtaa
     7381 tgtacattat ctgaacgaag aagatgccat ttaccgaaaa attttgatca attctatggg
     7441 cggagcgaat ccgttgaatc gtcatagttt gccggatgtc catttccgaa caaccgatga
     7501 gatgctaact gcatttcact ttttagggga agaaacagcg aaagagatcg tagtagaaaa
     7561 tacgaataaa atcgcagata tctgcgaaga agtgattcca gtcaaagatg aactttatac
     7621 accgaagatt ccaggttcag aagatgaaat atcagaattg agttacacaa aagcaaaaca
     7681 aatgtacgga gatccgttac ctgagatcat tcaaaaacga ttaaaaaaag agttgaattc
     7741 tattaatggt aatggcttct cggttattta tctgatcgcc caaaaactag tgcataaaag
     7801 taatgaagat ggctatctag taggttcacg tggctctgtt ggctctagct ttgttgcgac
     7861 gatgacagga atcactgaag ttaatccgtt agcccctcat tattattgtc cagagtgcca
     7921 atattccgag ttttttgaag atggtacgta tggttctgga tttgacatgc cagaaaaaca
     7981 atgtccaaaa tgtggtgcac gcttaaacaa agacggtcat gatattccat ttgagacatt
     8041 tttaggattc catggagaca aagtgcccga tattgatttg aacttctcag gtgattatca
     8101 agcagaagcc cataactata cgaaagtatt gtttggcgaa gactatgttt accgtgccgg
     8161 aacgattggt acagtcgccg acaaaacagc atatggctat gtaaaaggat acgaaaggga
     8221 caataatctg cagtttcgaa gtgcagaagt cgacagatta gcaaaaggcg ctactggagt
     8281 caaacggaca accggccagc atccaggggg gatcatcgtt attcccgatt atatggatgt
     8341 ctatgatttt acgccgatcc aatatccagc agacgatcaa aattcggaat ggaaaacgac
     8401 tcattttgat tttcactcga ttcacgataa tgtattgaaa ttagatattc tagggcatga
     8461 tgacccaact gttattcgga tgcttcaaga tttatcaggg atcgatccac aaacgatccc
     8521 tacggatgac ccggaagtga tgcggatatt tgccggtcct gaagtacttg gagtaagtca
     8581 ggaacaaatt tattccaaaa cagggacatt aggtattcca gaattcggca ctcgttttgt
     8641 tcgagggatg ctagaagaaa cgcatcctac aacttttgca gaactactgc aaatttccgg
     8701 actatctcat ggtacagacg tatggttagg aaatgcggaa gaattgattc gacgaggaga
     8761 tgcgacactg gcggaagtaa tcggctgtcg tgatgatatt atggtttatt tgatccatgc
     8821 tggattagat agcgggatgg catttaaaat catggaaact gttcgtaaag gacaatggaa
     8881 caagatccct gatgaattgc gagaaaccta tctatcggcg atgaaagaaa ataatgtgcc
     8941 cgactggtat atcgattctt gttcaaaaat caaatatatg tttccgaaag cccatgctgc
     9001 agcttacgta ttgatggctc ttcgtgtcgc ttatttcaag gtttatttcc ctattttgta
     9061 ttactgtgca tacttttctg tacgtgcaga cgactttgat ctagtctcca tgtgcaaagg
     9121 aaaagatgct gtgaaacaag caatgaaaga aatcacggac aaaggattag atgcttccgt
     9181 aaaagaaaag aatcaactga cagtgctgga gttagccaat gaaatgctgg aacgaggatt
     9241 caaatttgga atgattgatt tgtataaatc ggatgccgtt aactttgtta ttgaaggtga
     9301 tacgttgatc gcaccattta gagctgtccc aagtttggga accaacgtgg caaaacaaat
     9361 cgttgaagca agaaaagacg gtccattttt atccaaagaa gatcttgcca cccgcggtaa
     9421 agtttcaaaa acattgatcg aatatatgaa cgacaatggc gtattgaaag atctaccaga
     9481 tgaaaatcaa ttatcgttat tcgatatgct ataagcatct gttttcctgt ctgcttgtaa
     9541 ttttaagcag agtatggtat agttcttata ggtaatgata ctaactgtga gtgagcggat
     9601 ttttcgctca ctctttttaa tggaattacg cacttaattt tttaaggagg cgaaactttt
     9661 gagtagcgtc gttgaaacag tcacagaaat ggtgacaccg attttagatg aacaaaattt
     9721 tgaacttgtc gaagtcgaat ttgtaaaaga aggaaaaagc tggttcttac gagtatttat
     9781 tgacaaagaa ggcggtatcg atatcgaaga atgcgccttt gtcagtgaga aactgagtga
     9841 aaaacttgat gcagcagatc cagatccgat tcctcaagct tatttcctgg aggtatcctc
     9901 cccgggagca gagcggccat tgaaaaaaga aagcgactat gaaaaggcac ttggtgatta
     9961 tgtccatgtc tccttatacc aaccagtaga tggcgaaaaa caatatgaag gatttcttca
    10021 atcatttgat gctgaacagc ttacattgaa aatacgcata aaaacacgag aaaaagaatt
    10081 gacttttgac cgaaaaaata ttgcaaaagc tcgcttagct atccaatttt aatcacggag
    10141 aggaatcaga aaaaatgagt aaagaaatgt taaatgcgtt agacgcttta gaagctgaaa
    10201 aagggatctc aaaagaaatc gtgatcgatg ctttagaagc agcattggtt tctgcataca
    10261 aacgtcacta tggccaagca caaaacgtcg aagtggaatt tgatcagaaa aaaggaaaga
    10321 tccatgtcta tgcggtaaaa gaagtaacgg aagaagtgat ggattcacag ttggaagtct
    10381 cattgaaaga tgcactttta atcaatccag catatgagat cggtgatacg atccgttttg
    10441 aagtaacacc aaaagatttt gggcggatcg ctgcgcaaac ggctaaacaa gttattttac
    10501 agcgtgtacg tgaagcagaa agaacgatta tttataacga attcagtgct tatgaaaaag
    10561 acatcatgca aggaatcgtc gaacgccagg ataaacgtta tatttatgtc aatcttggta
    10621 aaattgaggc agttctttct aaacaagatc aaatgccaaa tgaattttat caaccacatg
    10681 atcgaatcaa agtttatgtt tcccgagtag aaaatacgtc taaaggtccg caagtatttg
    10741 ttagccgcag ccatccagac ctattgcgcc gcttatttga gcaagaagta ccagaagtct
    10801 atgatggaat cgttgaaatt gtcagcatcg cccgtgaagc tggcgatcgt tcaaaagtgg
    10861 cagttcgctc aaatgatcca aatatcgatg cagtcggtac atgtgtcggg ccaaagggac
    10921 aacgagttca agcaatcgtc aatgaattaa aaggcgaaaa catggatatc gtcgaatgga
    10981 atgaagatcc agctgtattc attgcaaatg cattgaatcc tgcacaagtc atggatgtga
    11041 tcttcgatga gacaaatcca aaggcctgca cagttgtcgt tccagactac caattatcgc
    11101 tagcaatcgg taaacgcgga caaaacgcac gtttagctgc taaattgact aaccataaaa
    11161 tcgatatcaa atcagaatct gacatgacgg aattttatga aaatcaagaa caacaaaaag
    11221 aaacagaaga actgcatgat gaagcaatcg ttcagtcaga tttaacagac gatgaatatg
    11281 aaacaattgc attcaacaat gaaacggtcg aagaaaaacc agaagcttaa ctttagtaag
    11341 gaggcaaaga gatgaaacaa aggaaaattc ctttacgcaa atccgtggtt tcaggtgaaa
    11401 tgaaacctaa aaaagagatg gtccgaatca cccgttcaaa agaaggggaa gtatcgatcg
    11461 atccaactgg aaaaatgcct ggacgaggag cgtatgtctc acttgagccg gaagaagtac
    11521 aacaagcttg ggataaaaaa attctcgatc gagtactcga aacaacatta acagatgagt
    11581 tttatcaaga acttcttgac tatgtcactc accaaaaagc acgtaaagag ttatttggaa
    11641 aatgaataca gttaatcggc aaaaagccat gaatctcatc ggtctagcga tgcgcgcggg
    11701 aaaactaatc acgggtgaag aactgaccat tgctgaaatc cgcaaacaaa aagcaaaatt
    11761 ggtgtttgtg gcaaccgatg caagcgagaa tacaagaaaa aagataaaag ataagagttc
    11821 atattatgaa gttccttgct ttgagctatt ttctgaagca gaaatcacgc agatgatcgg
    11881 aaaacctcgc aaggtattcg gaataatgga tgccggattt gcgaagaaga ccaaggagct
    11941 aattgaaggt taggaaggtg attgcatggg caacaaaaga atctatgaat tagcaaaaga
    12001 actcaaaaaa tctagtaaag acgtcgtgga aaaagcacaa caacttggca tcgatgtgaa
    12061 aaaccatatg ggtgcgatta ctagcaatga tgagaagaaa ttgcaacaag cgttcaaaaa
    12121 cccaagcacg aaacaacaag ctgcacaaca agcaagcaga aaaccagcca atcaacagcc
    12181 gaacgatcaa cgtaaaaaag aaacaacgaa caatcaacaa aaaaataaaa ataaccgcag
    12241 ttatcaagat cgcggtcaag ggagcggtca agtgaatcaa ggaaaaaaac caacaacaaa
    12301 ccaaaataat acgggaaata ccagcagcca aaatcgtcaa ggaacgagcc aaggcggcaa
    12361 tcaaaaccgt actaacaata acaacaaacc aaacaataac aatcgcggca aatttaataa
    12421 taataaccgt aatcgttata ataaaaaagg taaaaaaggc aaacagcaac aatcaactaa
    12481 accagctgta ccaccacgca aattccgtga attgccagat gtattagagt atactgaagg
    12541 tatgaacgta gcagatattg cgaaaaaaat tcatcgtgag ccagcagaaa tcattaaaaa
    12601 attatttatg cttggtgtca tggtcaatca aaaccaagca ctggataaag atacgattga
    12661 actgttagcg acggactatg gcatggaacc acaagaaaaa gtccaagttg acattgctga
    12721 tatcgataaa ttcttcgagg cagaagaagt cgatccagat aaattagtct cacgtccgcc
    12781 agtcgtaacg attatgggac acgtagacca tgggaaaaca actttattgg atacattacg
    12841 ccattcacgt gtaacaagcg gcgaagccgg cggaatcaca cagcatatcg gtgcctacca
    12901 gatcgatatt gatggtaaac caatcacttt cttggataca ccaggacatg cggcctttac
    12961 aagtatgcgt gcacgtggag caagtatcac agatatcacg atcttagttg ttgctgcaga
    13021 tgatggcgtc atgccacaaa cagttgaagc aatcaaccat gccaaagctg caggcgttcc
    13081 aatcatcgtc gctgtaaata aaattgataa accaggtgca aaccctcaac atgttatgca
    13141 agaattaagt gagtatgagt taattcctga agcatggggt ggagaaacga tctttgttga
    13201 gatctcagcg aaattcggcc aaaacatcga agaattgctg gaaatgatcc tgttagtcgc
    13261 agaagtagaa gatttaaaag cagatccaac acaacgcgca atcggaacag tgatcgaagc
    13321 acgtcttgat aaaggtaaag gtcctgtgtc aaccttactt gtccaagaag gaacacttca
    13381 tgttggtgac ccaatcgttg tcggtaatac ttacggacgt gtccgtgtaa tgacaaacga
    13441 tcttggccgt cgcgataaag aagcaggacc tgcaacgcca gtagaaatca ctggcttgaa
    13501 tgatgtacct caagcaggtg accgtttcgt agtcttcgaa gacgaaaaga cagcacgtgc
    13561 agcgggtgaa gaacgcgcga aacgtgcatt attagaaaac cgtgctgcca caagccgtgt
    13621 gactttggat aacttatttg aaagcctgaa agaaggcgaa ttgaaagaag ttaacgtaat
    13681 catcaaagca gatgtacaag gttcagcaga agcactggct gcatcattga agaagattga
    13741 tgtggaaggt gtccgtgtaa aaatcgtcca ttcagctgtc ggagcaatca atgaaagtga
    13801 tgtaacctta gcagcagcca gcaacgctat tatcatcggt ttcaacgttc gtcctacacc
    13861 gcaggcaaaa gtgcaagctg atactgaaga agtcgatatt cgtcttcacc gtatcatcta
    13921 taaagccatc gaagaaatcg aaactgcgat gaaagggatg ctagaccctg aatttgaaga
    13981 aaaaatcacc ggtcaaatga tcgttcgtga aaccttcaaa gtttctaaag ttggtacgat
    14041 tgcaggtgcc tatgtaacag aaggatatat tcgtagagat agcggtgtcc gtgtaatccg
    14101 tgacggaatc gtaatctatg aaggccaact tgcaagtctg aaacgattca aagatgacgt
    14161 gaaagaagtc aaaatgggtt atgaatgtgg tgcaatgatt gaaaaattca atgacatcaa
    14221 agtagatgac gtaattgaag gatttatcat ggaagaagtc aaaaatgact aaaaactgat
    14281 aaaaggaggc caatcattat ggctaactac cgtgaccgcc gagtcggtca agaaatctta
    14341 aaagaagtca atgatatttt gcgcaaaaag gtccgtgacc ctcgtgtgga gaacgtaacg
    14401 atcacagatg tgaaagttac gggggactta cagcaagcaa cgatctatta cagcatttta
    14461 tctgacttag catcagataa gaaaacagca caagatggat tgaataaagc atcaggattg
    14521 attcgtcgag agttagggca caatctaagt atttataaga cacctgaatt gttctttgaa
    14581 cttgatgaat cagttgtcta tggcaaccat atcgatgaat tgcttcgcga cttgaacaaa
    14641 gactaatcaa aa
//
LOCUS       DAZUMI010000073        14502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000073 DAZUMI010000000
VERSION     DAZUMI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.074"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACG81C_001890"
     CDS             complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACG81C_001890"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5065043.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1349..1984)
                     /locus_tag="ACG81C_001891"
     CDS             complement(1349..1984)
                     /locus_tag="ACG81C_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5065044.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2250..3302)
                     /locus_tag="ACG81C_001892"
     CDS             complement(2250..3302)
                     /locus_tag="ACG81C_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5065045.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3315..4253)
                     /locus_tag="ACG81C_001893"
     CDS             complement(3315..4253)
                     /locus_tag="ACG81C_001893"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5065046.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4704..5354)
                     /locus_tag="ACG81C_001894"
     CDS             complement(4704..5354)
                     /locus_tag="ACG81C_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065047.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5454..5963)
                     /locus_tag="ACG81C_001895"
     CDS             complement(5454..5963)
                     /locus_tag="ACG81C_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS5065048.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6164..7033)
                     /gene="scrK"
                     /locus_tag="ACG81C_001896"
     CDS             complement(6164..7033)
                     /gene="scrK"
                     /locus_tag="ACG81C_001896"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5065049.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(7053..8174)
                     /locus_tag="ACG81C_001897"
     CDS             complement(7053..8174)
                     /locus_tag="ACG81C_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5065050.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(8176..8571)
                     /locus_tag="ACG81C_001898"
     CDS             complement(8176..8571)
                     /locus_tag="ACG81C_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065051.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(8590..9387)
                     /locus_tag="ACG81C_001899"
     CDS             complement(8590..9387)
                     /locus_tag="ACG81C_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5065052.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(9389..10177)
                     /locus_tag="ACG81C_001900"
     CDS             complement(9389..10177)
                     /locus_tag="ACG81C_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5065053.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(10191..10667)
                     /locus_tag="ACG81C_001901"
     CDS             complement(10191..10667)
                     /locus_tag="ACG81C_001901"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5065054.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(10739..13396)
                     /locus_tag="ACG81C_001902"
     CDS             complement(10739..13396)
                     /locus_tag="ACG81C_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5065055.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(13460..13819)
                     /locus_tag="ACG81C_001903"
                     /pseudo
     CDS             complement(13460..13819)
                     /locus_tag="ACG81C_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(13997..14317)
                     /locus_tag="ACG81C_001904"
                     /pseudo
     CDS             complement(13997..14317)
                     /locus_tag="ACG81C_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         4578 a         2996 c         2081 g         4847 t
ORIGIN      
        1 tatagttatt gtttgcgctt ttatacggaa tttagttcat cattaatgtg gaattcgatt
       61 aggtgtctat taattatcaa gatgattctc tacaaaattc catagaaaaa tcaagattct
      121 acaattcgaa ttctctttca catggaaata aaggctagta aaaaaacagg agttgtgaca
      181 aaagacttgt catacctcct gtttccttgc gacttgaagc tgaatgcttt gcgccacaac
      241 cttttcctcg gtgttctcaa agcaactcct cgaaataatt cataactgtc caaaaatatg
      301 aaaagctatt tttgtcagtt gctcattatt ttgtcggagc tgaacgcttt gcgccacaac
      361 cttcctctat ctatgtcgct gaaaaaataa atgaagctga tgcgaatgct tgatcttttt
      421 atatctttta ggttgcttct gataaaattg ttgatgatga ttttcagctg gccaaaaagt
      481 ctttgcttgg cgaatctctg taacgatagg acggctaaat cggttagatt gaatcagttg
      541 ttctcttgat ttttcagcca attgcctttg cttttcatta acaacaaata gaattgaacg
      601 atattgtttc ccccgatctt gaaattgacc aaaggcatct gtagggtctg aaatttgcca
      661 ataaatctcc aacagctctt cataggttat ttgacgtgta tcaaatatga tctcaacagc
      721 ttcaacatgc ccagtatatc caccactgac ttgatcgtaa tttggttttt tggtctctcc
      781 acctgtataa ccagataaca ctgagattac acctggtttt gtttcaaaag gctcaaccat
      841 acaccagaaa cagccaccgg caaaaatcgc ccgttcttgg tataagggtg aagttatctt
      901 atgtttggca taatcataag cctttgcact cgctgactcc cctgtgattc ttaaataaaa
      961 atccatcgca tctggtgtca aattatctcg tagtgctagc ggacgcaagt tgacctctag
     1021 tttttctatc acctgcttta tcgacatttt tgctaggtct tcttttgcct cttcaaattg
     1081 tgttctttcc cattctcttg tatttcgatt taaaataata ttataaaggt ctatttccat
     1141 ctgctccaca tctaacatga agtcaccctc tttcaagttt tcttcttatt cattcaaaaa
     1201 atgactacct gaaatttagc acaacgcata acaagttaag actaacttgc ttttcgctct
     1261 cctcatctct attcttagtc gaacacatcc acacgaatgg ttacccatag agaaattctt
     1321 ttacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa
     1381 tcatcggcaa atagttaaag aaaaaactta agcgttgaaa gaagcccaat tgcttaaaga
     1441 aggcaaactg actaatcgac ggaatccgtg ctaatccgta aagacctgca attagtaaac
     1501 taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa
     1561 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat
     1621 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
     1681 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
     1741 tttttgatgt tccaataaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
     1801 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
     1861 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg
     1921 tcgataaatc actaatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa
     1981 tcattttctt ctcctacttt ttagtgatta ttcgatacat ccaacaatca aattttttct
     2041 cattttaaca catattttta ttctagttgc cttttgaaca atcaataaca taaaggtgag
     2101 tttcttctac attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct
     2161 cctactcaaa aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc
     2221 attgattgaa aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga
     2281 tcatacagtt taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg
     2341 atgactaacc aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac
     2401 ggaccaacaa gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat
     2461 agagcgacag gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt
     2521 tttctaaata gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca
     2581 cctgacttat taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc
     2641 atcgcagccg caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt
     2701 gaaataggag agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc
     2761 ggttgtaagg tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca
     2821 aaagggaata aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac
     2881 acaatcgaca ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat
     2941 gctgtcagca tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca
     3001 cccaaatcag gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc
     3061 atcattctca tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt
     3121 gttgctagtt gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta
     3181 aaatatttta aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt
     3241 gctgtaccag cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc
     3301 attcaatctc tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc
     3361 aagaaatcac aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca
     3421 ttgatataat cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga
     3481 taatgtaacc caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg
     3541 acatctagtt caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc
     3601 accattttat gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg
     3661 catagtccgt tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg
     3721 tcagaaaaat gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct
     3781 gcttcttttc catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc
     3841 tgaccaggac cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt
     3901 ggtacaaatt tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct
     3961 gtatcttttt tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg
     4021 gtaaatagaa taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt
     4081 ggtagttttg cttctacctc tttaccatta aaatttgctt gtaatccatg ctctttgatt
     4141 tgttggacat gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc
     4201 attaacccaa agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct
     4261 tcctcctttt tctttctatt atattcattt gagaatcatt tcacaacctt gttttgttat
     4321 tcttctctta tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata
     4381 aaaactggaa tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata
     4441 gaaacattca tagaaaaaac ctcactcctc tgattataaa agagggggca ttaacctcgt
     4501 gtatactaaa accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat
     4561 tgtagtacga tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa
     4621 caatacactt atttaaaaac ttctttccca gattttcttc catttttaca gttataacta
     4681 gtattatcat gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact
     4741 tttttattaa ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca
     4801 accggaatta tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat
     4861 atttcattgc catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta
     4921 ttgagttgat aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca
     4981 gtaatgacca ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcatttca
     5041 cctaatgaat caattatttc ttgcccttta gctctaatat attgatctct attacctatc
     5101 tcactcttat ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta
     5161 aattcgcttc tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac
     5221 tctgatttat tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca
     5281 aaattgagcc tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta
     5341 tattcatcat tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac
     5401 aaaaactagc tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact
     5461 tttctccgat tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa
     5521 ggcttaaaat ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga
     5581 tagatttctg cctcagcaaa aacgtctaaa atatcatcag tggtcataga caaggcgatt
     5641 tttaattttt ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg
     5701 tctactatcg gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat
     5761 gcgtcaaaaa cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg
     5821 ttcttatcct gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg
     5881 cctagctcaa aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc
     5941 aataacctat cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg
     6001 tactttttgt cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgataa
     6061 ggcgctgggt atcatgagtg aatgatattg ctgaatacaa atagtccgac cacattaagt
     6121 tgatcggact tcataagcat tttattgact aatacttttt tgcttaagaa ttgattctaa
     6181 catcttttgc taaagttaag catcctatag ttcctgcatt atctgctaac ttaggtgtga
     6241 caatgtattc ttcgatattt ggcgtatcga catagccgtt aattagttct aaaaaacgtc
     6301 tttgtacttt ctttttcaga tgttcttgtt gcatgacacc accaccaaaa attataatat
     6361 ctggtgaaag catcagggtc gtattacagg cacattgtgc aatataatcc gcctcgattt
     6421 cccagaacgg atcttctata gaaagatctt gtcccttggt tcccgtacgc atctcgatag
     6481 cgggtccaga tgccaaccct tcgagacaat catgatgaaa aggacaagta ccagagaaag
     6541 tatctcctga atgtctctta actaacgtat gccccatttc tggatggcta aacccttcaa
     6601 caaaaatccc atcttgtata gctcctccac caattccagt tccaacagtg taataaacaa
     6661 cactggataa accttttcca tgacctgaaa catactcccc gtaacaagca gcattcacat
     6721 cggttgtcca ggcaattgga atttcgaaat gcttattcaa cgttccaaca aaatcaaaat
     6781 ttttccaggc agtttttgga gtagaggtga tatgtccata ggtctttgat tcacgatgga
     6841 tatcaatcgg tccaaatgac cctactccaa tgctttctaa ttgttcttta tagtgattaa
     6901 agaaatcaat taccaaactc attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt
     6961 ctaaaacctt taaatcatca ttgccaatgg cacaaacaaa tttagtccca cctgcttcta
     7021 ttgagccata catttcttac tccaccgtcc ttttatctag gaattcgttc ttccctgtct
     7081 aatagttttt cgaaaaactc atgtggttcc ttttggtacc aataggcaac agaagaaata
     7141 tcgtcttgac gctcataaag cttgatgtca tcatttccta gggtttggaa agtaacacgt
     7201 aaattttctt tgaaactaat ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt
     7261 aaagcatcat cgccaaaacc atgaactgga tttggattag gttttccagc actaaaataa
     7321 tcccgggtgt gatctctttt agattgaaaa ggatagccca taaatagcgt attaaaattt
     7381 tgagcatatg ccctttctcc aaagttcttt ttatggaaag cccaagcgcc gccaaaataa
     7441 tcttctgctc ctgtagatgt tattgtaggg aaatcttcat ctccatctat ataaaactta
     7501 aattctcctt ccccccacca gtatctttcc aatgcgcaca aagcaaagta ggttcctaca
     7561 taatacccag tgcctttgat tccatcaata agggtgtaat cttttccttt tgtcgtgatg
     7621 ggttcacggt tccatttagc atgaaaatac atcgcatcat ccggtacttc agtcaatgca
     7681 taattaaatg tataaaaaat gtgcttaata aactcaggat gttcattcgt taaagtaatc
     7741 ttagcttttt ttcgaaatgg catctcaaaa tagcaattca tccctgatgt tggattcact
     7801 acgattggta aggaattcac ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct
     7861 aatggacttt ctacagaagg agtggattca tcatcccaat aaatacgcaa aattaaatca
     7921 cgtaagacga aactcccagc gattgttttg tctgtcactg tcatccaaaa atgacgtaaa
     7981 attccagggc cttccatttc tgctaaagta attgtctctc cagcatttat cggaacagaa
     8041 ggtgaccctt ttctcccaat tcctagatga ctcgctgtca ttgccgcttt ccctttttca
     8101 ccatttttat tttctggtga aatagttcta ctttcctctc ccttgtggag ccatatatta
     8161 tttaaacaat tcattttatt cgtcctctat cctttcttca cttttttgat tattcatata
     8221 gatgatttgt tgttgtgcct ccgcaataat tgctccaata tctaaattat catttcctaa
     8281 ctgcaaagcg attgacattg ccaagggaag attcattcct gcaattacgt gcactctgtc
     8341 agaaatatag gtaaaaaact tttgatttac acttcctcca gccatgtcag ttaaaacaat
     8401 tatttcgtct gtagtatcca ctaactcttt aatttgttca tctatatcat caatttcttc
     8461 gacatatgca cataagctac taatttgatt atgcatacct gttaaaaact ctagtgtctc
     8521 tttaaaaccc tcagctagta ttgaatggct agcaatgatc aatcttctca taattcaaat
     8581 ccttttcctt tacgctaata ttccaaatgc agccataacc atcgcaaaaa caataacgag
     8641 tagaatcaaa atggtcatac tgattttttt cttactgagt agataatatg ctgcagcagt
     8701 aataattact ggaattaatg ttggtaaaat tttgtccaac attggttgga ttttcatact
     8761 aacttcacca aaagtgaatt taagtggcgt tgaaacactg ataactgaag caattaatga
     8821 tccaactact gttagcccca atactgaagc agcttcagtt aaataattaa tcttttcacc
     8881 gtattcagta acaatcttca ttccctgctt ataaccaaat tcaaagcctc tcattcggaa
     8941 aaagaagatt accatgatta aaatgaacca taaaaagatt cctacaggat tattttctaa
     9001 agccatataa gccgcaattg atcccatgat ggttgggatc atcgtcatca aaagagagtc
     9061 cccaatacca gcaattggac ccatcgtacc aactttaaag tcattaatcg catcttttgc
     9121 ttctaatcct tgcttttcct ccattgcaac acaagcacct aaaataatgg ctgccagcca
     9181 aggctgtgta ttgtaatact gaaagtgatt atttaaagct tcgatgtatt catcatcatc
     9241 tttgtataat tttcttaaaa taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt
     9301 ttgataatta aaggtacaaa ctgacatcaa ccaacgatag ttcgctttcc taagatcctt
     9361 tttcgttact tgatattttt cgctcatttt attcatcctc cccaataaac ccaacatcag
     9421 aagctcccgc agaagcagtg atttgtgcat ctttttcagc tctatgatac acattaaatg
     9481 ctaaagcgaa tcctatgatc gccgctccaa taataggtac cttcaaaaaa gcggctaaga
     9541 aaaatccaac aagtaaataa ctaatgtatc gacctgtagg cataaagcgt aacaacatcg
     9601 caataccaac agcaggcaac atttttccgg ctaatgttag cccatccata aaccaattag
     9661 gtaaaacatc caaaacgtaa ttaacagcag actgtcctaa agtaacggca ataaatactg
     9721 gaattgctgc agataaacca aagaaaattg gccccaacca caaaacacta ttcattttct
     9781 taaattctcg tttattcgca tattcctgtg atttgcgaac aacaaaacca tttaagattt
     9841 tgacaattac atctagttgc acccctaaca tacctacagg tagtcctatt gctaatccgg
     9901 cttctactcc aagctggcta ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg
     9961 gtacagatga gccaccaaaa ccggcaactc ctaatgacat caattgaact gtaccgccaa
    10021 ctatcaaacc tgtttttata tcacccatca aaattcctgt gaataatccc caaaaaactg
    10081 cgtaatttac aaagatcatc ggaatcccat aataatcaac atgtttaata aatgctaata
    10141 tagtcagcat aataatttga aaaatagtaa aatgcattat ccttactcct ttatcctaat
    10201 tttgattgaa tttcctgtgc cttatccgcc ggaacaaatt gtgtaacaat ttctacacca
    10261 gaatccaaca attttttata cgtgctgata ttttcatctg atgcataggc tctagaagat
    10321 agctgtaatg cattatcttt aacaacagta ccgccaacaa ttagtcggtc aattctaata
    10381 ccgttatcta ctaaatgaag gattgtttcc ggctctttca cgatgacaaa tactttttcc
    10441 tgatcgtatt tccccttttt aaaattcgca atcgctgttt cttcagtgat aattgataca
    10501 gccattccag caggtctagc taaacgcata cttgattttt ggatgtcgtt atttgcgaca
    10561 gaatcatcga tcaccatcac tctgtttgct ttagacattg gtgcccattg cgtgacaata
    10621 atcccatgca ataaacggtt atcaactctc gacataacaa tactcataaa tacttcctcc
    10681 tttatctttc attttcttac aagcaattac catgccaaaa caaaaagaac ttgataaatc
    10741 aagctctttt tacataatca taaacgtatt taacctcatg aatcgggatg tcgacactat
    10801 aataatgttc aacgacactg aaagattgct taaaatgtgt ctgaaatttt tgaaattcct
    10861 cactcgtttc attgaaataa atttcatctt ccaaagtatt ttgttttact aatcgttcaa
    10921 ttaagcaact aatgtgaaca tataatccga aacacgtatt attatcaaat gaaattccta
    10981 gatcattttg taagatgtat atagcatctg aaacccgttc taataattta gtcggattta
    11041 atatggtcaa ttgctccatt aaattcgtta gactaaaatt cttcagaata ttattgttca
    11101 aaactttcag ttcattatca ttaaagtgtc tattcaattg attggagaat ttacttatat
    11161 tcattcctaa aatcatatct tcaattgaaa agaattctac atcttctaga tgtggattta
    11221 gtgttccaac aataaaaaga atattgttct ctttacgaag ctccgataaa tagttttcat
    11281 tcactaaaga gtaatagtca cacgtttcta tgacaagatc agtatccctt ggaaagctat
    11341 cttcaagtat cttttttaat ttaaacgaag ttcctaatcc gcttgcacaa gaacagacaa
    11401 tcatatcctt cttaatattt gatgattcat aaatataatc cacattttga ttttctttta
    11461 cttgttcaag aatctgttta agcggtaaat ctaataatat ctgatttgca acatcaatag
    11521 ccattcttgt tgaaacattg tttagtagtc cgaatttttt ttgagcgtct agttccaaat
    11581 ttagataaat tttctccagt gaacccatat caacaagtaa aataatttct tcatatccgg
    11641 tacactcttg taagtatcta ttaattttgt ctgcaatctc actacttgaa gtcattagtg
    11701 gcatatcaat ggcgtcgtat atatatctct caacgatcct attagcagta cttgctatac
    11761 aggttgccgt tagatctcca tgagccaaaa tcagtgctaa acgtttttgc tgaaattctt
    11821 tactacacat taacttaaat aaaataacca aaacattttt ttgaattttt gttaaataaa
    11881 tatcccctat ttgaataaaa cgattgacta attgaacttc tcgactacac caactattaa
    11941 tatcattgat gaaggaatat aatttatcgt ccgatgttaa ttggatttga taaatccaag
    12001 aattcaaaaa gatagacgtt atttgaagat aataatagtc aaattctatt tgaaaatctc
    12061 tctctatttc cttaacatac tgtctaaatc gaatctcttg ataggtggga tatgttaaag
    12121 ctgaattatt ttggatatca acattctttt gtctaaaata tctatcatat ttctcaaaaa
    12181 gctgctgctt atcagtgtct ttcttccacg tttgataaag atactctata agttcgttga
    12241 tattaaaaac ttcgtctact ttttccaaat catccatttg aatatatttt aattcattct
    12301 gtcttttcat aaccttcatg ccgttgaaat tcacccattg tggtaaatga tacatctgaa
    12361 tataaagttc ttccaaatca tctaagaaat aaaaggcatt ggcacaggtc aattgaatga
    12421 tatttttcaa attgccaaca ttttcactaa agcgatagtt taataaaaaa tctaataatt
    12481 gttgagatat cagtattttc tttgctagtc tttcttcttc tgatttaaaa aatatcttga
    12541 ttaactgttt tctttcataa agagaacgac tgtctaatgt tggaagtgtt agctttacag
    12601 gtattcttct caacaaagtt gataatagtt tatcctcagg tggttctgat gttgcaaata
    12661 taatccagca acttgatgtg taccagtttt cattatcacc catccgatga aatttcctcg
    12721 tatccataaa ctgaaaaagt ttctcctgac attcattgct caatgcatga atttcatcta
    12781 aaaataagac tccattatta gctaattctg ctaaaccctt tcttccatct tcggctcctg
    12841 tatatgcacc ttttacatgc ccaaaaaaat tggacaagaa aagttctgga ttattggcat
    12901 attcactaca attaactgaa atgaaagatg ccttctcttc tatataatta ttggccaaca
    12961 aatccttata cacataattt gctaaatagc tttttccagt tccagtctcg ccatgaagta
    13021 aaatgggtaa gccatttggc ggatagtaga ttgctgcttt aatttgagca attacatttc
    13081 gcagacttcc ttgacttccg ataacccttt ctattccaac cctaaaagag ctatcatacc
    13141 atttttccgc ttcctctgaa gagctaaaaa tttgttgatt tggaactttc tccggtaaag
    13201 aaaagaagat tactggacga gtattgattt ttccaaattc tccatctttt acgaattcat
    13261 tcaaatattg agagacaata ttacgactaa catttaattt ttcagatatt ttctgtgctg
    13321 tattgttccc ccatttattt ttaatctcag agttctctat gacataattg acaatgcgtt
    13381 cttttaattc ttccatcaca gcacctcctc gacactattc gaattttgcc gacagtattt
    13441 ttccataaat atgacttgct aaaaaggctt tgtttagatc attcaattgg aacgttgctt
    13501 gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca taccccaaaa
    13561 tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt aattcacgat
    13621 acaacatcgt ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa
    13681 ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagcccc aataaataat
    13741 ttttatattc tgacgcatcc attttactac ggagattatc tgccgcacta aataacttct
    13801 gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt gctatatacc
    13861 ctcttatttt agcatactca gttttagaaa aagcaaaaaa ataaagaaac ttattcttga
    13921 taacaagcta cgtgacgtat atccctaaca caaaaatagc catatccttt ggtttggata
    13981 cggctatttg atattactca ttcaattatc aaatcacagt aggtgtgaat ctcatcggga
    14041 tcggcaatat ctacttctga ttaggatcaa aggtgatttt ctcaataaag gtgtgataca
    14101 aagctttaac ttgcttcttc tcactctgga taagaaagca atccaaacta gtcaatatct
    14161 gttggacatc tttgacttga atcggttccc cttgttgttg aaagatccct aagatttcat
    14221 tctctcgaat ttgtaactgt cttcgttttt cagtcaattc atttaaacga tcttttaaca
    14281 tcggaaacaa ttcgggacta tcttccaaag cagcttcctg tttttgctct aatagttaga
    14341 ttttgcgtac cattttcttc gtttattttc cttcgattgc tgattttaat atagtctaaa
    14401 ggaaatttat atttattgta ttctttataa cttcaagaga acttcgtttg agtattactg
    14461 tattttttta attccataag aaaattagtt attacctgcc ac
//
LOCUS       DAZUMI010000074        14496 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000074 DAZUMI010000000
VERSION     DAZUMI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.075"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..885)
                     /locus_tag="ACG81C_001905"
     CDS             complement(<1..885)
                     /locus_tag="ACG81C_001905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5065056.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTS"
     gene            complement(1262..1900)
                     /locus_tag="ACG81C_001906"
     CDS             complement(1262..1900)
                     /locus_tag="ACG81C_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065057.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1904..3886)
                     /locus_tag="ACG81C_001907"
                     /pseudo
     CDS             complement(1904..3886)
                     /locus_tag="ACG81C_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4066..4731
                     /locus_tag="ACG81C_001908"
     CDS             4066..4731
                     /locus_tag="ACG81C_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065058.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5128..7161)
                     /gene="brxL"
                     /locus_tag="ACG81C_001909"
     CDS             complement(5128..7161)
                     /gene="brxL"
                     /locus_tag="ACG81C_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFS5065059.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7279..9801)
                     /gene="pglZ"
                     /locus_tag="ACG81C_001910"
     CDS             complement(7279..9801)
                     /gene="pglZ"
                     /locus_tag="ACG81C_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFS5065060.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9917..13549)
                     /gene="pglX"
                     /locus_tag="ACG81C_001911"
     CDS             complement(9917..13549)
                     /gene="pglX"
                     /locus_tag="ACG81C_001911"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFS5065061.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13613..>14496)
                     /locus_tag="ACG81C_001912"
     CDS             complement(13613..>14496)
                     /locus_tag="ACG81C_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS5065062.1"
                     /translation="NTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELFDW
                     AETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKAD
                     PYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIV
                     DQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAA
                     NLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEIN
                     QKLKEQLQENTEITIQF"
BASE COUNT         4502 a         2770 c         2053 g         5171 t
ORIGIN      
        1 tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta cattagcgtc
       61 tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt ttgtgtctgt
      121 tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt tttggttttt
      181 caccatttta accacataat attccgtagt ataagttgaa gcattggtag aagtgatcac
      241 atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt
      301 tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag tatcagttga
      361 cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct tcacatcttt
      421 tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat ggaaagattc
      481 ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac cttcttgaaa
      541 ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa
      601 ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg aatcgatttc
      661 tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga tcaactgttg
      721 cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt gttcgctgat
      781 tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc ctgacaataa
      841 gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc aattctccta
      901 cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat catcaagaac
      961 acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa tccagaaagt
     1021 tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac ttcttttgtt
     1081 accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt agaacaaatg
     1141 ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca cttttatgtt
     1201 cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt tcatttgttt
     1261 tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc gtgaccacga
     1321 taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc tccttatttc
     1381 gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc ttcaataaga
     1441 ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata ggcgtactta
     1501 gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc ttatctgaaa
     1561 catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt tcatattcaa
     1621 tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag cgcttctctc
     1681 ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa ctgaattgat
     1741 cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct ccaactacag
     1801 tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt ttgtttcgaa
     1861 tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct ccattcatca
     1921 gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga ataaaatcaa
     1981 tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata agaaaaagga
     2041 agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct ttatacgttt
     2101 caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg tagacaaaat
     2161 atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt aaaaatatct
     2221 gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca gtctgagtat
     2281 acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc tcaaataacc
     2341 tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct ccgctgagaa
     2401 tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc ggataatgtt
     2461 cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt ataaatagaa
     2521 atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc aggtcatttt
     2581 ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt tctttttcgg
     2641 ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga taattcagat
     2701 ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct gatacattga
     2761 ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct gctgatgcaa
     2821 aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga taaaatacat
     2881 gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac tgtgaagaag
     2941 aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa aacaacaatc
     3001 caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa gcgatttttt
     3061 caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag aaaacgtaca
     3121 tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca ccaatgattc
     3181 ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac aatacttgat
     3241 gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat aagaaaaagg
     3301 atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga taattatata
     3361 cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca aaaatattag
     3421 tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa atgaaccgtt
     3481 cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct tttattttga
     3541 tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc ttctcgtgag
     3601 aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt atcttgaagt
     3661 tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa gaaagctcag
     3721 tttctgatga gacattttca aataccatga ttagctgctc atattcttct tgcccaataa
     3781 aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat tcatttgtcc
     3841 gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata ccggtcacta
     3901 tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct tacttaaatg
     3961 attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa atgtataggt
     4021 catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa catcggagaa
     4081 aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg cgaaaaaatc
     4141 ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc agaccttgaa
     4201 atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc tattgaatca
     4261 gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa gaaatcttat
     4321 tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct cgtcttttgt
     4381 ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga tcgattcaat
     4441 ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt ggaaaattcc
     4501 atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc ttttcacgct
     4561 ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa acacgtcatg
     4621 aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat cctcatccaa
     4681 gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta agtaggagat
     4741 gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac aggggtacaa
     4801 attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga atgataggta
     4861 aaaaaatgac taactattct agtattaggc tatttgtatt attcttctgt tatttttata
     4921 tcaaaaaatc cagaaataac taaatatagg tatttctggc ttatagtata atatttacgc
     4981 cacagccccc aatgattctt tttggaatca tgatcaagga cggacataca caaaaatatt
     5041 ttgcccattc gatccgaata gattaacgac taatctagta agtaatatag attggtacgt
     5101 agtcaaacca gcaaatggaa aagttgtcta ttccacccca agggctttaa agacagcatc
     5161 ttccggcgtt tgataaaaaa tcaaattaaa ggtacccatt aattctgggg gtacagttcc
     5221 aatatcagca gcgcttatca taggtacaag tacttttttc gctccggcat cttttgctac
     5281 ttgtaaacag ttagctaaat tatctacctt aatcatagaa ccaccaattg taatatcccc
     5341 taaaataact aaacttggta atacaggttt atttaaggaa actgagcata aagctattat
     5401 agttgataac gataatgttt ttgtcatacc aatgccattc atatcttgaa catgaacgac
     5461 aaaatctttt gtcgtcaaac taatagactg actaacactt ccataattag ctcttaaata
     5521 tcggtatgcc gaatccatcg cttctcttgc tccagcatct gaattgatcc cagtacgttc
     5581 aaactttcca tttcccgaag tcatttgcgt ttccaattta taggtaccta attgcccagt
     5641 tttgccataa gataccgtgt acaaatgccc tggcttccct aacccttctg gaatcaaagt
     5701 tcctccacct tgttcaggaa cagaaacata ctcttctcgc tcttcttcta aatcaatgta
     5761 tgaaaaattg acatcataaa attccattcc accaattttc tttaattgtt cttttacacg
     5821 acgacgcatt tcaagagcaa atcttagtat ttcttcgaca tcactttttt caaatctacc
     5881 atcaggatac aacagtttca tcatcccaga tacaatccga cgaactgcaa tcgtatcccg
     5941 ctgatttaat tgagaaccaa gtttgaaata tttatcaaaa ctatcgccaa aactaacttt
     6001 tcgaagctca cgcatgactt cagaaaaata atctgtaatg aagccatagt catttgtaaa
     6061 aaaatctggt cgatatttag gaatttccca acctggtaaa taacaatgca tccgatccaa
     6121 aaaggctgaa tccgtcccca taacctccgg aaatggctca aataaattcg aagttttcaa
     6181 tattacatca acgctttgat taatatttcc tacaaaagcc attgatgctg aagcattctt
     6241 ttcttctttg cctctagcaa aagatcctga agccatgtaa tctttcatga tttgaacgcc
     6301 atcattatcc ttaaatttaa tcccagcaac ttcgtcaaaa gcaacacagt cccacatacc
     6361 tacaagtccc attgtacgac tagccatatt atagaaaagg tttgctactg ttgtttgtcc
     6421 acctgaaaca aggattgagt ttggtgaaat ttctttatat acatgcgatt tacctgtacc
     6481 acgtggtcct agttcacaga gattaaaatt attttcaatt aatggaataa gtcttgctaa
     6541 atgaagccat tttacccttt tattaaattg tgtaggttcc ataccaatgg accgtagaag
     6601 taagtcaatc cattcatttg tagtaaactc cttgcgagcc atcttgtaag cctcaatgtc
     6661 taaaccaggc atttggattg gatcaacctt ttttgcaata aaagggctat tccaacgatc
     6721 attctcatca taccaatatt ccatcgaaac aatacaccag atccccccac ctaataatcg
     6781 atcaaatttt ttcggatact cttcactaat ttgaacattt tttaatccca ggttagagaa
     6841 ttctgcttca taacaatctt tcttttcatt taaatgtaca gttactttat caataactgt
     6901 ataaccaccc aattcacgaa tttttgcttt tactttttct gcttcatcag gacgcacata
     6961 gtttcgagca agaatatttt taacattttc cacacctttt tcaaccaatg tttcatccaa
     7021 tgacgaagca tacataccaa gtaaatattc caatacataa acaggtacat tcgcaccttc
     7081 tttaattttc tttgttaaat cttttctgac aatctttccg ggaaagaatt cagataactt
     7141 taaaatttcc tgttgttcca ctagaattcc tccatgtgtt cagtcattat aatagcaatt
     7201 aaagtatatc atacttaacc agtaggcttg ataatacaaa agaaaaagta tccacaaaaa
     7261 attgtggata cctcgttttt atatatcaaa atcaaatgat tctgcaaaag gattatctat
     7321 aatgaatttt attcgctcgg tcaaatcatt tttttgattt tttatcacta aataataagt
     7381 atcttcaact gtatatcgac gatttacaaa ttcaaaaaca aatttagtaa accgctgatt
     7441 tgcatcttct gtttttgaat ccgcaacata gatttcttca tttgagatac gttcaccata
     7501 ttcatcttca aaataaagcc agaaagatgc ggggtcataa acatcactaa ttgcattttt
     7561 ttgatagaat tctaacgtgg ttgataatcc tgtaattctg cggttgctag tcattaattc
     7621 aattgctact ggttcctttg cattgctacc tttggctacc gtaatttcta gaacagggac
     7681 tagcatttct tgaatagatg accctccatg aacgtagctt tgtcccccgc cagctttctt
     7741 aaaaatactt gaagtaatcg ggacacaaat gtttctagtg tcatcatttc ctaaagaatc
     7801 tcccaaagag taatttttcg taccaattat ttcgtattct tcttcactaa gaataaaacg
     7861 acgttctaca cgatcttttt catttctaga tggattttca atcttatcta cttcttcaac
     7921 gtggcttctt ttataaataa atccatgatc agccgtaact ataaaacgat agatactgcc
     7981 atcattatgc aagcgattaa taagtcggga aatttcgctt atcgctaaat cacaggcctc
     8041 aaaaactcga ttttcattcg agtgatcacc tgttctatcc acttgatcgt gataaatata
     8101 aatgactgat ttgccattaa atacttctcg tacctcagcc ttattcatct taaccacatc
     8161 atcataattt aaagctacag catttgcatc tcgttgctgg agaatcgcat ttcgattgga
     8221 taaaccattc gtttttttcc catcaatcag tacttctgtt ccttcattgt aagttagttg
     8281 attgtttggt aataaggcgg ctttaccgaa ttcagtaact gaaggtaata cagaaagaaa
     8341 gctagccatg tttgttgaat acttcttttc tctaactaac ttacgttcta aattcatccc
     8401 taactcatat cgaaaagcat ctgaaataat aactacagtt ttaacagagc gtttcattac
     8461 agcttgatta taaaattttc gaatggatgg acgattctta aattcaaaag tttgattcca
     8521 aatttgtcct gattgatcaa gaaacttcga ataatttgct tcaactaatc gatacaattg
     8581 gccatatttt tgcttttctt ctatatgatc actattccaa ataaatttcc gataatgcgt
     8641 atcaacccaa tacatttcct tctcatattg agttagcaaa ttatccatgt tcatcggcaa
     8701 gcacaatctg tttttatcca caagataata agcatgtttc aataattgat actcataaat
     8761 ataatccgat gcgaaatgca ttttttcgcg ctgctttaaa ctatcaaaaa ttgtttgtcc
     8821 tgcaattttc gttgtagtgt cagaagcctt taatcgttct gcataaaaca ttaatatttt
     8881 tgcatgaatc tcagaaaata cgtctgcttc aattagatca tcaatttcta tactgtctaa
     8941 taatccatca ccatcaatag cctgatatac ttttttagat agctgttcaa agctttccat
     9001 gtaacgtgta tcattcataa actgatccat gaaagtaata atgtttgctt tcaaatgtag
     9061 tatttcaaat cctttgaact tcttcggcat ctcttgatca atttgataat aaaaatagtt
     9121 caaataaaga cacatcgtca gcttttccaa tgttggctgg ctatccgtaa agccaaattt
     9181 catttgaata aattcccaaa atttagctgt taactgatac ttatcaagtt cccttaaagg
     9241 attctcccta ctttctgtaa agctttttag aactacacgc agtaaatcat ttaatcgaat
     9301 acttttcgat ttcaacaaaa cagccattat cattaattca atcgtttctg atttattaat
     9361 tgtcatatgc gtactaaatt caccaaaact tctcatgcga tcctttgcat taaagaaatt
     9421 acgatactgt ttaactgtct gatgaaatct aagtggaata gccaattcat tcatgataat
     9481 cgctaagcgg tctgcactaa attcctctga ataattcaat acactcaaca aatgattatc
     9541 ttcatcttta tcatcccaac gaggaaatgg cgcatagatt aaatagttag tttcgggatc
     9601 ttcatattcc aataaacgct tagttttaaa ttgtgcgtcc ttttctaatc gatagatttt
     9661 tgcattttga atgaccaatg agtcaatttc ttcaagaaaa tcctgatttg aatcaaacca
     9721 aaaaatgatt ttcctacatc cataattagg aaaacgttga tttaattttt cttcaatttg
     9781 ttgcgtattt atatctgtca tcaatctcac ctaatcgttc tctttctata ccccttatat
     9841 tgacataatt tggataattt cgcacgcaat ttaagaataa aaaagaaagt tgttagaaaa
     9901 caactttctt agataactat ttttttggtt ctttgacccc ttttgcaaaa atttggaatt
     9961 tctcgccggt tttaggatca cgctgcaact tatcataatt gacaattaca ccatcatcta
    10021 agtctaattc aattcgtttt aatgctaagt gacccaactt ctcgtcatac tcacgaactt
    10081 cttttaattg cttctgaatc tttacaattt cttttgtcat tgcatttctt tcttttgggt
    10141 tatcacttgt tgaaatttgt tgctgacgca attcgattcg attgtcatac gctttttgta
    10201 gttcgtgtag gtattcagta cgtacttttc ctaattgatc tggtgtatag cgatgcaagt
    10261 acattaacgc tttaaagcca ttttgttttc ctgagtccaa ttgccaataa attggtcgat
    10321 tggagtatgt ttttaaatgg tctttgtaaa aatcttgtac gaagtaacga cgaataattt
    10381 ctcgacttgt ccctttgcca cctaaagtgt cagcgataaa ctggagattt tcttcaaggg
    10441 tttctttacc aaaagtaatc tcaacgaaat caatgaaacg attgacaatg tcaatttggc
    10501 tatccttgaa atactcatca tcggtgatga gtaaaacatt atcagtatca ggtttaaatt
    10561 tttgatagcg ggaaggatcc cattcgccgc cagcgaagat gagaccatct tcgtctaggg
    10621 agtaacgacc gaagatacaa cctattgaat aagaaataaa atccttaatt tccctcgcct
    10681 tatcaggtaa ccttgcagag atatattcat cttgaacctc ttttgttaat tctttttcaa
    10741 gagaataatg attaatgaat atgtcgttca gttcctcttc attcgatttt agagtattaa
    10801 attgattaac caactgccta cttagacagt taaatgcctc tgagagatct tttttaaaca
    10861 tataacttaa caaaatgttt cttctaaact cgtatgacgt ttcaagagca ttatagtctt
    10921 ttctagctat atcgatattt tcatccactt tttctcttat tttttttaat tttctctcag
    10981 atgtaacttt aagaaaaggt agttttccca ttggtccttc atggtaatcc aaggtgggag
    11041 aaataaaagt taaaaagtga ttaactaatt tagagtttac atagccaaat atttcttcaa
    11101 agtcagttat atcctttgag aacagaacag atcctttagt ttcaaaaatt gcctgattct
    11161 gcaatctaaa agatacttgt ccagatgaaa gtgtcgacca agtaattccc tttttaaaat
    11221 agtaattaag attttgtggt ctagatttta acttcccatt ttcattaaca taatttttta
    11281 tttcatctcc atcattctcc cagttaacca cgtattcatt gtttccatac cattttctaa
    11341 aacgaccacc tttagttgta ggaaaccatt tttttctaga ctttttagcg tcctctaatg
    11401 ttttagcatc aaactttatt ttgttaaatt ctacttcaaa ccaataccgc aaaaatttat
    11461 tgttatttcc ggttgccaac ccttgttttg catctgcaac atcgttagtt aattttcctt
    11521 gagcaaaatc agtgattacg ttatcgcttg cccagtaagc aatcggcatt cctgggattt
    11581 ttttgaagtt gtcttgatta gtactataaa aataatcgca ttcaccatgc tgatgaattg
    11641 cttctaacgt tttaattttt tgaacttcca ttccaccttt aaaatctgat aattttatat
    11701 agcttccatc ttgttcattt gaatttttta tcgtaaacgt acaaattgga acggttgctt
    11761 cttcaaaagc tgaatactcc atttgaatca aactagaaat gtttctttca ttaattaaaa
    11821 atgagcgtaa cttttcatag ctagaaatga acatccaaac aaatggcgtc ataaaggaag
    11881 catatccatt atcaatcagc atttggctat tcaatttaat aaacacagaa aatagatctt
    11941 ttttgacatc agaatagtta tcatttacat atttttttaa tttcttatcc attttattaa
    12001 gataaggcgg attagtgact accgctacat atttatcaac tagcatttca gccacgtcaa
    12061 gaatgtgcaa gtatttttct tgtatttcct tcatcccata aagaatatcc atattcgtaa
    12121 attcgttgaa ttttgatata tactctctta cttttcctaa gtattcttta gttattgcct
    12181 gattattttt atcgactaac ttaatgattg atccaagttc tttcccattt tcaaattgat
    12241 caaaaattaa ctctttgtat tcattccagc gttcactata cttatcattg ttcaattcac
    12301 taatacgctc ttgatactcg tctgtcactt taggagaatc aataaactct cttaaattcg
    12361 gcgatagact ttttttcaaa aaaagacgat cgtattgacg ccctttcatc ataattgcaa
    12421 aataagacaa ttgatacgct cttttatcga tttcaagacc atataaattg tattcaacga
    12481 ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag ataaatttcc atcaatacat
    12541 cgaaggcgta aactaaaata tgcccagacc ccattgcgtt atcgataatt tttagttctt
    12601 ctggttttaa attacttaca gatgttgctt gttcaaaagc atatttatct ggaattaagt
    12661 atttcaactt atttcttaat tcactattgg gataatgatc taaccaatac tttcctaaac
    12721 tattgtcaac catataacga acgacccaat ctgtggtaaa tagttgagtt gctgccggaa
    12781 tatcttgttt tttgacaatc cctttataaa tattcaccac ttgattatgt ggctcactat
    12841 tatagtactg atacaaccac ccaataatct ctacctgtcc accagcttga acatagaaat
    12901 catcctctgg aatacttaaa agatcagcga ttacaccatc ttttttaata taagaagctg
    12961 taaacaaaag ttctgtataa tcttctactt cttcaaatag ctcaggtaag taatcattca
    13021 attgattaca gatttttata aaaatcaatt gatacagctt gtccattgct tctgctgaat
    13081 tatctgtttt ccaagttctg acttgttgct gttcattact agataattct tcaaagatat
    13141 cggcatcata aatatctgta ataatatctg gctcaatttt cccacttatt tcactagata
    13201 atacacgata cgtgtcaggt aaataatcat ttacctccat aaaccgaatg gcaatcaatc
    13261 ggttaaacca cgtataagca acctcttcga ttaactcttg aaaagcttct gaataagaag
    13321 tgacagaagc acgttgtttt agttctgcaa tcaatttgtt tctttgcgct atttcttttc
    13381 cttgaattct tattcctgat ttttcgattt cttgtaaatt gctatcaatt ttcttcacat
    13441 cttcaatttt tgattcatca attcctaaac gttttgcttt agtcattact tcatcaatta
    13501 attttctacg ggcattgacc gcgaaattct ttatcgcttt cttatccatt tttctttccc
    13561 ttctatatgt aacctttgaa aagacaggca cctttttatg cctgtctttt cactagaatt
    13621 gaatcgtgat ttctgtattt tcttgaagtt gttccttcag tttttgatta atttcagctg
    13681 ttaactcatc aatatctgct gctgacttaa tagttttaac atgaacgtta agtgttctca
    13741 ttggtactgt tttctgttga atagtaggtt ttactatcgc tatttcttca gcttttgctg
    13801 cagagtctat ttcatctttt tcagtttcac gaatcaagtt tgctgcttca atttgtttac
    13861 gtgcttgttc ttcttgacgt ctgcggtttt cttcattatc gaaacgttga ttaaagatca
    13921 aatactgttc tgctacgttt aatgataaga aaacaagatc atctatagta ttcgcttgtt
    13981 caatttttgc tttcagttct tgaaattctt ggtcaactat tggtacaaat tgatccttaa
    14041 aatcttttcc ttctaataat ttaaatgttg cttgtttttc ttccgttaat ttatttaata
    14101 tttcctgact ttccaactct aacagttgat tatattgttc aataaattct tgaatcaact
    14161 catttagttt catcatatct gaataaggat cagccttttt caaaatggtt ttcatctcat
    14221 gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg attaatctaa
    14281 ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg tggaaatttt
    14341 taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt ttgattgtat
    14401 aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt tttatttgct
    14461 catattcata agcactaggg taggtataag tgtttt
//
LOCUS       DAZUMI010000075        14449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000075 DAZUMI010000000
VERSION     DAZUMI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.076"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACG81C_001913"
     CDS             complement(227..1114)
                     /locus_tag="ACG81C_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065063.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACG81C_001914"
     CDS             complement(1111..1980)
                     /locus_tag="ACG81C_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065064.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACG81C_001915"
     CDS             complement(2117..3403)
                     /locus_tag="ACG81C_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5065065.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACG81C_001916"
     CDS             3676..4254
                     /locus_tag="ACG81C_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5065066.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACG81C_001917"
     CDS             4254..5183
                     /locus_tag="ACG81C_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065067.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACG81C_001918"
     CDS             5180..6916
                     /locus_tag="ACG81C_001918"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5065068.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACG81C_001919"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACG81C_001919"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5065069.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACG81C_001920"
     CDS             complement(7812..8321)
                     /locus_tag="ACG81C_001920"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5065070.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACG81C_001921"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACG81C_001921"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5065071.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACG81C_001922"
     CDS             complement(9622..10713)
                     /locus_tag="ACG81C_001922"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5065072.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACG81C_001923"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACG81C_001923"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5065073.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACG81C_001924"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACG81C_001924"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5065074.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACG81C_001925"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACG81C_001925"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5065075.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4116 a         3144 c         2612 g         4577 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgt
//
LOCUS       DAZUMI010000076        14237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000076 DAZUMI010000000
VERSION     DAZUMI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.077"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG81C_001926"
     CDS             complement(<1..1835)
                     /locus_tag="ACG81C_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5065076.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG81C_001927"
     CDS             complement(1832..2536)
                     /locus_tag="ACG81C_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5065077.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG81C_001928"
     CDS             complement(2548..4857)
                     /locus_tag="ACG81C_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5065078.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG81C_001929"
     CDS             complement(5052..5516)
                     /locus_tag="ACG81C_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065079.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG81C_001930"
     CDS             complement(5516..6142)
                     /locus_tag="ACG81C_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5065080.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG81C_001931"
     CDS             complement(6149..6514)
                     /locus_tag="ACG81C_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS5065081.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG81C_001932"
     CDS             complement(6504..6842)
                     /locus_tag="ACG81C_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065082.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG81C_001933"
     CDS             complement(6832..7158)
                     /locus_tag="ACG81C_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5065083.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG81C_001934"
     CDS             complement(7139..7417)
                     /locus_tag="ACG81C_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5065084.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG81C_001935"
     CDS             complement(7419..8783)
                     /locus_tag="ACG81C_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5065085.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG81C_001936"
     CDS             complement(8796..9365)
                     /locus_tag="ACG81C_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS5065086.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG81C_001937"
     CDS             complement(9337..10524)
                     /locus_tag="ACG81C_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5065087.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG81C_001938"
     CDS             complement(10588..12315)
                     /locus_tag="ACG81C_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5065088.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG81C_001939"
     CDS             complement(12312..12764)
                     /locus_tag="ACG81C_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5065089.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG81C_001940"
     CDS             complement(12876..13256)
                     /locus_tag="ACG81C_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5065090.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG81C_001941"
     CDS             complement(13253..13639)
                     /locus_tag="ACG81C_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFS5065091.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG81C_001942"
     CDS             complement(13641..13919)
                     /locus_tag="ACG81C_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065092.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14237
                     /locus_tag="ACG81C_001943"
     CDS             13982..>14237
                     /locus_tag="ACG81C_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065093.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENIL"
BASE COUNT         3945 a         3058 c         2240 g         4994 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaa
//
LOCUS       DAZUMI010000077        14189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000077 DAZUMI010000000
VERSION     DAZUMI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.078"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACG81C_001944"
     CDS             <1..483
                     /locus_tag="ACG81C_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS5065094.1"
                     /translation="FGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIET
                     ALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNG
                     ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI
                     "
     gene            805..975
                     /locus_tag="ACG81C_001945"
     CDS             805..975
                     /locus_tag="ACG81C_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065095.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            989..1591
                     /locus_tag="ACG81C_001946"
     CDS             989..1591
                     /locus_tag="ACG81C_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065096.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1607..2284
                     /locus_tag="ACG81C_001947"
                     /pseudo
     CDS             1607..2284
                     /locus_tag="ACG81C_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2595..3092
                     /locus_tag="ACG81C_001948"
     CDS             2595..3092
                     /locus_tag="ACG81C_001948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS5065097.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3093..3509
                     /locus_tag="ACG81C_001949"
     CDS             3093..3509
                     /locus_tag="ACG81C_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS5065098.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3529..5073
                     /locus_tag="ACG81C_001950"
     CDS             3529..5073
                     /locus_tag="ACG81C_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065099.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5216..5440
                     /locus_tag="ACG81C_001951"
     CDS             5216..5440
                     /locus_tag="ACG81C_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065100.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5455..6324
                     /locus_tag="ACG81C_001952"
     CDS             5455..6324
                     /locus_tag="ACG81C_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5065101.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6305..7039
                     /locus_tag="ACG81C_001953"
     CDS             6305..7039
                     /locus_tag="ACG81C_001953"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5065102.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7072..7980
                     /locus_tag="ACG81C_001954"
     CDS             7072..7980
                     /locus_tag="ACG81C_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5065103.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7977..8519
                     /gene="sat4"
                     /locus_tag="ACG81C_001955"
     CDS             7977..8519
                     /gene="sat4"
                     /locus_tag="ACG81C_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS5065104.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8612..9406
                     /locus_tag="ACG81C_001956"
                     /note="aph(3')-IIIa"
     CDS             8612..9406
                     /locus_tag="ACG81C_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5065105.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9682..9813
                     /locus_tag="ACG81C_001957"
                     /pseudo
     CDS             9682..9813
                     /locus_tag="ACG81C_001957"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9889..10382
                     /locus_tag="ACG81C_001958"
                     /pseudo
     CDS             9889..10382
                     /locus_tag="ACG81C_001958"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10552..11448
                     /locus_tag="ACG81C_001959"
     CDS             10552..11448
                     /locus_tag="ACG81C_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5065106.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11547..11756
                     /locus_tag="ACG81C_001960"
     CDS             11547..11756
                     /locus_tag="ACG81C_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5065107.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11773..12045
                     /locus_tag="ACG81C_001961"
     CDS             11773..12045
                     /locus_tag="ACG81C_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5065108.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12047..12910
                     /locus_tag="ACG81C_001962"
     CDS             12047..12910
                     /locus_tag="ACG81C_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5065109.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACG81C_001963"
     CDS             complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACG81C_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5065110.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14035..14118)
                     /locus_tag="ACG81C_001964"
     CDS             complement(14035..14118)
                     /locus_tag="ACG81C_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5065111.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5018 a         2222 c         2928 g         4021 t
ORIGIN      
        1 tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact
       61 ttagttgaaa gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta
      121 gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct
      181 attacgtttg actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat
      241 attgatatat attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct
      301 aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg attttagaaa agtgaatcag
      361 acatttattt ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac
      421 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
      481 tgacaaatca tactatgata cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag
      541 aaatatagag agtaagattt taattaatta ttagggggag aaggagagag tagcccgaaa
      601 acttttagtt ggcttggact gaacgaagtg agggaaaggc tactaaaacg tcgaggggca
      661 gtgagagcga agcgaacact tgatctttta agttgctatc tttttaaggt caatagagta
      721 tacttatttg tcctaatatg gttttagcag tataattgac ttggtgaata ggtcatttaa
      781 gttgggcata ataggaggag taaaatgaaa aaatttattt atcgagtttt agaaaatgac
      841 gaagtggtgg ctatttttaa tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag
      901 acaatttctg ataagcaatt tgaaattgaa aaagtagata tagctgattg gttattgcaa
      961 ccgagagaat tttagaggtt ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag
     1021 caaagaacag aacttagata gacaactgaa agcgttagag ggcgtttcta aggtcttttc
     1081 agacaaatta agcggtcaat cggtcgaacg cccacaatta caagctatgc ttaactatat
     1141 tcgtgaaggg gatatagttg ttgttactga attagatcga ttaggacgaa ataataaaga
     1201 attaacagaa ttgatgaatc aaattcaaat taagggggca accctggaag tcttaaattt
     1261 accctcaatg aatggtattg aagatgaaaa tttaagacgg ctgattaata atttagtgat
     1321 tgaattgtat aagtaccaag cggaatctga acgcaaacga attaaagaac gccaagccca
     1381 aggaattgaa attgctaaga aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga
     1441 aaatgatcca cgtttacaac acgctttcga tttatttttg aatggttgtt ccgataaaga
     1501 agttgaagaa caaactggaa ttaatcgccg aacgtttaga aggtatcgat caagatacaa
     1561 cgtgacagta gatcaaagaa aaaaacaatg aaaagaggga tagttaatga gtacggttat
     1621 tttagctgaa aaaccaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
     1681 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
     1741 tggttttggg catttagtcg aattagcaga accaggtcat tataacgaaa agtggcagaa
     1801 ctggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     1861 taagggaaag cagtttaaaa ttgttgctga acttctcaaa aaggcaaata caattattgt
     1921 cgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
     1981 tgccttttct aaagataaaa cgtataaaag attatggatc aatagcttag aaaaagatgt
     2041 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     2101 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac
     2161 cttgaattta caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc
     2221 gactttatat ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt
     2281 tttcctgaat aattcatagt tttactaata gtctttccta acattgattt aatagtcttt
     2341 aaagtattct gcttcatcac ccattaggtg atgaaaaaag aacccctttc tgatttagcg
     2401 gacaaatact ttgacctaat ctcagaagat ataaaagaaa aaataataaa atatccgatt
     2461 tatactttgt ataaagaact aaagtagtta gcaaatcatt tacagcaaat cactatgttc
     2521 cacataccat tttacatact taccttatcc acgacacatg ttgagatagt atcgaagata
     2581 aagaaagttg ctccatgttg tttttgcgag atggggaaat caaagccaca ttgacaacca
     2641 tgatgaataa gcttgctttc agtcataagc taatcctaga accacttttc aagtcagtta
     2701 gccaaatcga tgaagaaagc gaccgtgaaa gaatggacgc tattgataag ctaatggagc
     2761 aactattgga agaacgcaat acccttattg cactgatgtc caaaggtttc ctagaacctg
     2821 ctctttttaa tcaggaacga aatgttttag atagtgagat taaaaatctt actaccgaaa
     2881 aaacaaacct ggtaacgaac tctgcgagtg gggttttgcg agcaaacgag ataaaggacc
     2941 tcattaatta tgtgtcagca gataatttta atggtgacta cacggaagaa ctatttgaag
     3001 aatttgtagt gaacatcatt gtaaattcca gggatgagct gacattcaat ttgaaatgcg
     3061 gtctttccct gaaagaaaag gtggtgagat aaatggcata tattccatat ggatacaaaa
     3121 ttcaagatgg agtggttact gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg
     3181 agaaatacat atcaggacta tcccttacag tggctggcga acaggcaggt attgaaaaga
     3241 cacattctgt gatggggcgc attttgaaaa acgtcaacta ccttggaaat gatacgtatc
     3301 cagcaattat tgataaagag atatttgata aagctgaaga agttagagat aaacgtgcaa
     3361 aggatttagg acgagtggta gagcttgccg ctttcacctc tccccctccc aaagaacgat
     3421 tcaaaatgaa aaaggcagat aataagatgc cagtggatcc ttttgaacga gcagaatact
     3481 tatacagtct gatagaaagc gaggaataaa gtgacagaga aaaatataat ggttattcct
     3541 gctcgtaaaa gagtaggaag tacagccgca aaagaaaaga taaagaaact tcgtgttgct
     3601 gcctattgcc gtgtttctac agaaacagaa gaacaaaatt ctagctatga ggttcaggtc
     3661 tcacactata cagagtttat aaagaaaaat aatgaatggg agtttgctgg catctttgca
     3721 gatgatggta tctctggtac gaacactaaa aagcgtgacg aatttaatcg catgattgca
     3781 gagtgtatgg acggtaacat cgacatggtt attacaaaat ccatcagccg atttgcgcgt
     3841 aacaccctag actgccttca atacattaga cagctcaagg ataagaacat atcagtttat
     3901 tttgagaaag agaacatcaa caccacggat gccaaaggtg aggttttgct gactattatg
     3961 gcatctttgg cacagcagga aagccagagc ctttcacaaa acgttaagct tgggctacag
     4021 taccgatacc aacaaggaaa ggtgcaggtc aaccataagc gatttatggg ctacacgaaa
     4081 gatgaagatg gaaatttaac tattgtcccc gaagaagctg agattatcaa acgcatctac
     4141 cgagaatacc ttgagggtca gagtctagtg ggcattggtc gagcccttga aaaggatggt
     4201 attttaacag cagcgggaaa accaagatgg cgaccagaat cagttaagaa gatacttcaa
     4261 aacgaaaaat acatcggaga tgcccttctg caaaagactg tcacagtaga ttttctgacc
     4321 aagaaacgag tgaaaaatga agggcatgtt ccccaatatt atgttgaaaa tagccatgaa
     4381 gcgattattc ctaaagagtt atttttgcag gcgcaagaag aaattcatcg gagaagcaat
     4441 atctacacag gagaaggcaa gaacaaaaga atttacagta gcaagtacgc tttaagtgcc
     4501 atcaccttct gtggagattg tggcgatatt tatagacggg tctattggaa tatccacgga
     4561 agaaaagaat ttgtctggcg atgcgtgact agaatcgagc aaggtcctga agtttgtaag
     4621 aaccgaaccg taaaagaaga tgaactttat ggtgcagtaa tgactgcgat taataagcta
     4681 cttgcaggtg ggaacaatat gataaagaca ctggaagaga atattcatgc tgtgattggc
     4741 gaaaccacag aataccaaat ttcagagatt aacaactcac tagaggaaaa acaaaaagaa
     4801 ctaatcaagc tggcgaacaa gggtcaagac tatgaccact tagcagatga gattgatgag
     4861 ctgagagaca agcgacagat ccttttagta gaagatgcct ccctcagtgg cgagaacgag
     4921 cgaatcaatg agctgattga atttatccgc aagaacaaat tccgcacctt agaatatgat
     4981 gataagcttg taaggaagat aatccagagc gttacagtct atgaaggcca cttcgtcata
     5041 gccttcaaat ctggcatcga aatggaaata tgaaaaccag aagtaaatag cccatgactc
     5101 tacagtagag ttgtgggttt tcttttgctt gctgattata ataatatttt aataactatt
     5161 gtgtttatca accaaatggt ttataatatt cttagcgtaa acttggaggt gctgtatgac
     5221 tacggcagaa atgattaaag aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc
     5281 tagacgtatt ggccagactc cacagaattt caataaaaaa ttacaacgag aaacagtaac
     5341 cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat
     5401 tttaccagat ggagaagaaa taaaaatatc taacgaataa aggaggatgt ttatatggta
     5461 ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt tgccttttat agaaggcatc
     5521 gtattagggg gctcacgtgc aagaggcacc catacagaaa attctgatat agatatcgga
     5581 atctattaca attcagactc atttgacctg acagcgatta atcaaattgc tacagagttg
     5641 gatgatgaga atagaaacaa ccttgttgta cctcccggag cgtggggtga ttgggttaat
     5701 ggcggtggat ggttagttat aaacgggtgt catgttgact tgatattacg tgatataaaa
     5761 cgggtggaac aaataatcaa agatacggag caaggaattg ttactgccaa ttatcagacc
     5821 gggcatcccc atggttatat aagtgctatg tatcgtggag aattagcgat tagtaaaata
     5881 ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact
     5941 gctctaaaga aaagcttgat gaactttttt atatttgaag ctgaattctc tttaatgttt
     6001 gtaaaagcaa atgcgggggc agaggataaa tattatattg caggccatgt ttttcgtatt
     6061 atttcatgct taaatcaagt actatttgca tgtaataatg cttattgcat taacgaaaag
     6121 aaagctataa aactgcttga aacttttgaa tataaacctg aaaaatatgc cgaaagagtt
     6181 aatcatattt ttgaagtact cggtctttct ctttttgaat gctacgacat gaccgagaaa
     6241 ctttataaag aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     6301 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     6361 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     6421 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     6481 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     6541 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     6601 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     6661 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     6721 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     6781 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     6841 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     6901 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     6961 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     7021 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     7081 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     7141 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     7201 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     7261 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     7321 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     7381 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     7441 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     7501 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     7561 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     7621 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     7681 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     7741 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     7801 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     7861 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     7921 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     7981 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     8041 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     8101 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     8161 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     8221 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     8281 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     8341 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     8401 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     8461 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     8521 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     8581 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     8641 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     8701 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     8761 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     8821 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     8881 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     8941 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     9001 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     9061 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     9121 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     9181 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     9241 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     9301 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     9361 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     9421 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     9481 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     9541 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     9601 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     9661 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     9721 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
     9781 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
     9841 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
     9901 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
     9961 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
    10021 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
    10081 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
    10141 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagcct
    10201 atatcactgg ggttttatga acagaactgg tacttaatcg gttttgcagg ctaagaaatg
    10261 aatataggaa ttttcgtgtg gatcggatta aaagcatttg catagaagaa gagctctgtc
    10321 aaagccatga tggctctttg gaacaaatat taaaacaaat gctttcatat aaaaaactat
    10381 atgccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc
    10441 tcaaaataaa tataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta
    10501 tcacaaatca caagtgatta atcacaaatc acttgtgatt tgtgattctt aatgatacaa
    10561 tattactata caaaaaaaga atggggcgta gttatggaga aggaaaaact aaaaatactt
    10621 gaagaattaa gacgtatttt aaacaataaa aatgaagcaa ttattatctt gaataattac
    10681 tttaaaggtg gtgttggaaa gtccaaatta tcgactatgt ttgcttactt gacagacaaa
    10741 ttgaatttaa aagttttaat gatcgataag gacttacagg caacattgac aaaagactta
    10801 gcaaaaactt ttgaggtaga attgccacgt gtcaattttt atgaaggctt gaaaaatgga
    10861 aacttggctt cttctattat tcatttgact gataatttag acttgatccc tggcacgttt
    10921 gatttgatgt tactgccaaa attaactcgc tcatggacgt ttgaaaatga aagtagattg
    10981 cttgctactc ttttagcacc tttaaaaagt gactatgatc ttattattat tgatactgta
    11041 ccaacgccaa gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct
    11101 ttacaagcag aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat
    11161 ttgcaagaac agtttaaccc tggactagat atgattggtt ttgttcctta tttagttgat
    11221 acggacagcg caacgataaa atcaaacctg gaagaactgt acaaagaaca taaagaggat
    11281 aatttggttt tccgaaatat tatcaagcga agtaataaag taagtacttg gtctaaaaat
    11341 ggcattacag aacacaaagg ctatgacaaa aaagttttgt ctatgtatga gaacgtattt
    11401 tttgaaatga ttgagcgaat cattcaatta gaaaatgaaa aagaatagaa tcacaaatca
    11461 caagtgatta atcacaaatc acttgtgatt tgtgataggt gatgataaaa taaatagtaa
    11521 gaagaaatag aaagaagtga gcgatcgtgg gaaatttagg cgcacagaaa gcaaaacgaa
    11581 atgatacgcc aatcagcgca aaaaaagata taatggggga taagacggtt cgtgttcgtg
    11641 ccgacttgca ccatatcata aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag
    11701 ttatggatca agccttagaa gaatatatac ggaaatattt acctgacaaa ctttaaaagg
    11761 agtgaaaatg aaatggcagt tacgtatgaa aaaacatttg aaattgaaat cattaatgag
    11821 ttatcggcaa gtgtttataa tcgggtatta aattatgttt tgaatcatga actagatact
    11881 aaaaatactc gtttactaga agtgaatctt ttaaatcaat tagaagtggc acaagaagtt
    11941 gatttatttc aacaaccatt tgaagaatta caagctattc atgagtattg gcggtcaatg
    12001 aatcaatatt caaaacaaat tttgaataaa gagaaagtgg cttaacatgg cgaatattac
    12061 tgacttcacc gaaaagcaat ttgaagatcg tttagaaaag aatgttgaac gactaactaa
    12121 aaatagacta gcggttgaat cgccaaccgc ttttttactt ggtgggcaac cagggtcagg
    12181 gaaaaccagt ttgcgatcag caatttttga agaaacacaa gggaatgtta ttgtcattga
    12241 taatgatacc tttaaacaac agcacccaaa ttttgacgaa ttagtgaaac tctatgaaaa
    12301 agacgtagta aaacacgtta ccccttattc gaatcgcatg acagaagcac ttattagccg
    12361 tttaagcgat caaggctata atttagtgat tgaaggaaca ggacgaacaa cagacgttcc
    12421 cattcaaaca gccacaatgc ttcaatctaa aggttatgaa acaaaaatat acgtcatggc
    12481 agtaccgaaa attgaatcat acttaggaac gattgaacga tatgaaacca tgtatgcaga
    12541 tgatccaatg acagctaggg caacaccaaa acaagcgcat gatattgttg tcaaaaactt
    12601 accgaccaat ttagaaaccc ttcataaaac gggcttattt agcgatataa ggctttacaa
    12661 tagagaagga gtgaaactct attcaagctt agaaacgcct tccattagtc cgaaagagac
    12721 cttagaaaga gaattaaatc gtaaagtatc agggaaagaa attcaaccga ctttggagag
    12781 aatagagcaa aaaatggttc aaaatcaaca ccaagaaacc cctgaattta aagcaattca
    12841 acaaaaaatg gaaagcttgc agctacctac accaccaata cccaaaacac ctaaacttcc
    12901 aggtatttaa accttaaaaa aggaaaagag tagttaccaa aaaacggtaa ctactctttt
    12961 tttataaaaa catttcctca tgttggggtc ccgagcgcct acgaggaatt tgtatcgata
    13021 agaaatagat ttaaaaattt cgctgttttt ttctaatttc attttgaggt gaattaattg
    13081 gtagttatta gaggtgccct atacctaata atttatctac attcccttta gtaacgtgta
    13141 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
    13201 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
    13261 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
    13321 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
    13381 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
    13441 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
    13501 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
    13561 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat attcccaaca
    13621 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
    13681 acacgagtgt tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
    13741 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
    13801 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
    13861 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
    13921 ctgaagtgat tacatctata aataaataca gaagttaaac gatttgtttg taattttagt
    13981 tatctgttta aaaagtcata agattagtca ctggtaggaa ttaatctaac gtatttattt
    14041 atctgcgtaa tcactgtttt tagtctgttt caaaacggta gatgttttat ctacattacg
    14101 catttggaat accaacatga cgaatccctc cttcttaatt acaaattttt agcatctaat
    14161 ttaacttcaa ttcctattat acaaaattt
//
LOCUS       DAZUMI010000078        14129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000078 DAZUMI010000000
VERSION     DAZUMI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.079"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG81C_001965"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG81C_001965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5065112.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG81C_001966"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG81C_001966"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5065113.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACG81C_001967"
     CDS             8118..9086
                     /locus_tag="ACG81C_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5065114.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACG81C_001968"
     CDS             9104..10009
                     /locus_tag="ACG81C_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065115.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACG81C_001969"
     CDS             10006..10812
                     /locus_tag="ACG81C_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065116.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACG81C_001970"
     CDS             10969..11937
                     /locus_tag="ACG81C_001970"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5065117.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACG81C_001971"
     CDS             complement(12254..12778)
                     /locus_tag="ACG81C_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5065118.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACG81C_001972"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACG81C_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5065119.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4001 a         3048 c         2787 g         4293 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZUMI010000079        14037 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000079 DAZUMI010000000
VERSION     DAZUMI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.080"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACG81C_001973"
     CDS             279..1127
                     /locus_tag="ACG81C_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5065120.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACG81C_001974"
     CDS             complement(1298..1639)
                     /locus_tag="ACG81C_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS5065121.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACG81C_001975"
     CDS             1725..2432
                     /locus_tag="ACG81C_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5065122.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACG81C_001976"
     CDS             2419..2742
                     /locus_tag="ACG81C_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5065123.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACG81C_001977"
     CDS             complement(2831..3868)
                     /locus_tag="ACG81C_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5065124.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACG81C_001978"
     CDS             complement(4315..5298)
                     /locus_tag="ACG81C_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5065125.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACG81C_001979"
     CDS             complement(5714..6484)
                     /locus_tag="ACG81C_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065126.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACG81C_001980"
     CDS             complement(6477..7412)
                     /locus_tag="ACG81C_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065127.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACG81C_001981"
     CDS             complement(8006..9655)
                     /locus_tag="ACG81C_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5065128.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACG81C_001982"
     CDS             complement(9850..10746)
                     /locus_tag="ACG81C_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5065129.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACG81C_001983"
     CDS             complement(10739..11419)
                     /locus_tag="ACG81C_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5065130.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACG81C_001984"
     CDS             complement(11420..12202)
                     /locus_tag="ACG81C_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5065131.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACG81C_001985"
     CDS             complement(12195..13112)
                     /locus_tag="ACG81C_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5065132.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACG81C_001986"
     CDS             complement(13115..13399)
                     /locus_tag="ACG81C_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065133.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4153 a         3003 c         2452 g         4429 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    13921 cattgaataa gtttgaaaag ccttgattta taaaggtttt tccgtaaata tattttgtta
    13981 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagt
//
LOCUS       DAZUMI010000080        13823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000080 DAZUMI010000000
VERSION     DAZUMI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.081"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..2247
                     /gene="acm"
                     /locus_tag="ACG81C_001987"
     CDS             82..2247
                     /gene="acm"
                     /locus_tag="ACG81C_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5065134.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2519..3004)
                     /locus_tag="ACG81C_001988"
     CDS             complement(2519..3004)
                     /locus_tag="ACG81C_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5065135.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3094..3439
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3484..4125
                     /locus_tag="ACG81C_001989"
     CDS             3484..4125
                     /locus_tag="ACG81C_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5065136.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4144..4779
                     /locus_tag="ACG81C_001990"
     CDS             4144..4779
                     /locus_tag="ACG81C_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5065137.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4797..5612
                     /locus_tag="ACG81C_001991"
     CDS             4797..5612
                     /locus_tag="ACG81C_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5065138.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5931..7367
                     /locus_tag="ACG81C_001992"
     CDS             5931..7367
                     /locus_tag="ACG81C_001992"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5065139.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7556..10003
                     /locus_tag="ACG81C_001993"
     CDS             7556..10003
                     /locus_tag="ACG81C_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5065140.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10233..11300
                     /gene="rlmN"
                     /locus_tag="ACG81C_001994"
     CDS             10233..11300
                     /gene="rlmN"
                     /locus_tag="ACG81C_001994"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5065141.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11290..11496
                     /locus_tag="ACG81C_001995"
     CDS             11290..11496
                     /locus_tag="ACG81C_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065142.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11493..12596)
                     /locus_tag="ACG81C_001996"
     CDS             complement(11493..12596)
                     /locus_tag="ACG81C_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5065143.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13148..13696
                     /gene="aac(6')"
                     /locus_tag="ACG81C_001997"
     CDS             13148..13696
                     /gene="aac(6')"
                     /locus_tag="ACG81C_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5065144.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4732 a         2299 c         2933 g         3859 t
ORIGIN      
        1 tgggggagga attgaagaaa ggatttttga gagatgatat agtaggtttt atttttagtt
       61 aaaatagggg agatagaaaa aatgaaaaag tgtagaaata ttattttatc aatgttattt
      121 attattacta atataacatc actgattcct gttcatgtct atgcggatgc aggcagagat
      181 atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga tggaggaaac
      241 ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg tgattatctt
      301 tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa agaaatccca
      361 ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc tgcgcaggtt
      421 gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca atttgagata
      481 caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac cgtgacgagc
      541 ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc ttctagcgtt
      601 ttttattata aaacaggaga catgctacct gaagatacta agcacattcg atggtttttg
      661 aatattaaca ataacggcac ttatgttgaa caaccagtta aaatcagtga tgaaatccaa
      721 agtggacaaa gattagatcc aagtacgttt gaaattaatc aaatacactt aggtgaacag
      781 aaggtttata gaggagaaga gggaatccaa caatttttac aagatttccc cagcgctaca
      841 tttaattttt cagtaactga taattacatt gaaataacta taccaaaaaa ctttgtcaat
      901 cttaggaaaa taatggtgag ttataagacg atcatagaaa acccagaaca aataaatttt
      961 gagaatcatt cggaagcttg gtttaaagaa tttaataaac ctgctgttga tggagaaagt
     1021 ttcaatcata cagttaaaaa tatttcagct tctggtggag tcaacggtac agttagaggc
     1081 gaattgaaga ttttcaaata tatcaatgac acagagattg gtatcccaaa tgtgactttc
     1141 gaattaagac gagcagatga acagcctatt caaggacagt ctagtatcct gttgacttcg
     1201 aatgaacagg gagaagctag tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa
     1261 aaagaagcac ccaattggat tgattttgat ccactaagtt caaatgaact caaattctcc
     1321 ataaacgaaa atgatacaga gggagtcagt ttacctattt ataacaagaa aaaggttacg
     1381 aacatcacag caactaaaat ttggaacggt ggaaccactc caagaccgag tatttatttt
     1441 aaattattca gagcaagtac aaacaattgg gaaccagttc ctgatgcaga aacaaaacga
     1501 ttagacaatg gaataacttc agtgacttgg gaagatatcc aacagtatga tgatagtgga
     1561 aacgaatata cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta tgtcccttca
     1621 ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg agaataaaga agtcatttca
     1681 gtctcaggac aaaaaacatg ggaagacaat gagaaccaag acggcaaacg accgacgacg
     1741 atcacagtaa atctattagc cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa
     1801 gatgattggc gctatcgttt cacaaatctg ccaaaataca gagacgggca agaaatcatc
     1861 tatactgtaa cggaagacaa cgtgcccgaa tacagtacga cgattgacgg gtataacatc
     1921 aaaaacagtt atacaccgga aaaaacaaat atatctatac caggtagcca tataacaccg
     1981 tggaaaaatt ttccagagaa gacagagaga gagaaaatta gaaatctatt ctctcaaaaa
     2041 ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa gaagcaatta tattagtaag
     2101 agagctgtac ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt
     2161 aaaaaatcta gttttgctgt aatcattggt ttattgatag taactatctg tgctgggata
     2221 ttcttacaaa aatataaaaa agtttagtta gaatataata ttttttgtta aatatgactg
     2281 aaatcaaaag aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt
     2341 cttttttcgt agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag
     2401 ttttcagcgt tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat
     2461 tttgtgctaa ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt
     2521 acaaattttc ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa
     2581 aatccataat caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat
     2641 caatagcagc atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg
     2701 gagtcactgt acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag
     2761 ataagcgctt ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag
     2821 gcaagatgac aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt
     2881 gcggaccagg tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt
     2941 tttgaaaaga gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac
     3001 tcatcgcgca tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa
     3061 aaataatcat gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc
     3121 gttaggtgtt tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca
     3181 catcttggag tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct
     3241 gcggtcgttg ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag
     3301 gtggaaccac gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct
     3361 gaacggcaac gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca
     3421 cgtataatac gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag
     3481 aagatggagt atttgattga agttatgccg caattattag taggagcact cacaacattg
     3541 aaattatttt tatttacctt gattggctca ttaccattag ggattgtttt agcattagga
     3601 ttgtctttgc attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg
     3661 ggaacaccgc tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc
     3721 gtttttgatc gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt
     3781 gcagagatct ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa
     3841 gttttgcaat taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag
     3901 attgttttac cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc
     3961 tatatattag gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg
     4021 actttactgc ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca
     4081 atcgtaatga aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga
     4141 aatatgattg aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat
     4201 ttgaatctaa tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt
     4261 aaaaccacgc ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat
     4321 ttagacggtg taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc
     4381 gtattccaag acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca
     4441 ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg
     4501 gcttcattag ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa
     4561 aaacagcggg tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat
     4621 gaaccaacaa gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca
     4681 ttgaaagaag atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat
     4741 gtcggtgatc aattactaaa agtttcccca attcattaag aaaggagaag atagagatga
     4801 aaaaaaccag ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga
     4861 acaaaaaaac ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag
     4921 gtttagacga tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat
     4981 ttgatattga tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc
     5041 ccattgactg gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga
     5101 acggctattc caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg
     5161 aaaacgatca agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga
     5221 ataataagag attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac
     5281 cagaagtatt aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg
     5341 aagcattgat cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg
     5401 cagactatta tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt
     5461 atgagagtga ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa
     5521 tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat
     5581 ggtttggcga agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg
     5641 tcacagtccc tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct
     5701 gaatggcagg ctcacaacct cttggtaaac ggtgttcaaa agccagtttt tcggtattaa
     5761 gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta ctactcgaaa
     5821 ctaagcgact ttttcacaac ctcctttaca taattttatt ttgttgtgga aggggtttcg
     5881 tgttattcta aaagaggaac gattgaaggt tataggaaag gaagagatga atggaacaca
     5941 aagaacttac ctcatttcca aaagattttc tgtggggctc tgcttctgca gcgtatcaag
     6001 tagaaggagc ttggcaagaa gatgggaaag gagaatcagt ctgggatcga tttgtacgga
     6061 tacctggaaa gacatttaaa ggaacgaacg gtgatttggc agtagaccac tatcatcgtt
     6121 ataaagaaga tattgcttta atgaaagaac aaggtctgaa agcttaccgc ttctctgtag
     6181 cttggacacg gatttttcct aatggacgag gagaagtcaa tcaagctggg ctcgcttttt
     6241 atgagagatt gatcgacgaa ttgattgaaa acgatattga acctatgttg actttatatc
     6301 attgggatct tccacaagca ttgcaagatg aatataatgg ttgggaatca cgtcagatca
     6361 ttgaagattt cactcattat gcagaaacat tatttgaggc attccgcggg aaagtccatt
     6421 actggattag tttgaatgaa caaaatatct tcacttcatt aggttatttg ttagcagcac
     6481 atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt gctaatttag
     6541 caaatgcatc tgtcatcaat aaatttcacg agttagaaat gcctggaaaa atcggaccaa
     6601 gctttgcgta tacgccaaat tatccaattg acagtaatcc tgaaaatgtt ctggctgctg
     6661 aaaatgcaga agacctgatg gccaattatt ggatggatgt ctatatgtgg ggaaaatatc
     6721 cgattgctgc catgaggttc ttagaagaaa aaggatgggc accaacgatt gaagctggcg
     6781 atgcagaatt gctagaatct gctaaaccag attttcttgg gatcaattac tatcaaacgg
     6841 cgacgaatgc attcaatcca ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa
     6901 aaaaaggttc atcagaagaa actggagttc ctggaatgta taaaaaagtt gagaatccat
     6961 ttgtcgaacg aacaaattgg gactgggaga ttgacccaga aggattaagg atcggactgc
     7021 gtcgtatcac aagccgttac cgcatgccgg tactgattac ggaaaatggc ttaggagagt
     7081 atgacaagtt gacagaagat aaacaaatcc atgacgacta tcgaatcaat tacttacaaa
     7141 gccacgtcaa agccatcaaa gaagcaattt cggatggagc agaagtccta ggctattgca
     7201 cctggtctta tacagatttg cttagctggc tgaacggtta tcaaaagcga tatggatttg
     7261 tatatgttga tcaagacgaa acacaagaag gatctttgga acgatataag aaagacagtt
     7321 attactggta ccaaaaactg attgaagaaa atggagagaa cctttagttc acatacttaa
     7381 aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta
     7441 agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact
     7501 ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc
     7561 acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca
     7621 gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt
     7681 cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc
     7741 aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat
     7801 taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta
     7861 tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc
     7921 aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga
     7981 caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat
     8041 tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt
     8101 cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca
     8161 ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag
     8221 aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga
     8281 aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc
     8341 tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg ttccaatcga
     8401 cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag atacaccaaa
     8461 acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg gtacctcatt
     8521 agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga cagttgattt
     8581 gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat
     8641 cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag
     8701 cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa aagtaagtaa
     8761 aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg tccggatgga
     8821 agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa agatcagtat
     8881 ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat tactagaagt
     8941 acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga agatgcctca
     9001 cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg ggattatcac
     9061 aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg atccgaaaat
     9121 ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg tgacgaaccc
     9181 tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac gatatgaaaa
     9241 attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc aacagaaaaa
     9301 cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg tagatgaatt
     9361 agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc gtttagcgca
     9421 aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa gtgtggatgt
     9481 tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg
     9541 tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag ggcgaggaga
     9601 tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag cattcatttc
     9661 tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag ctcattatga
     9721 agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac agccacaaga
     9781 tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa gtatttccct
     9841 tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag atgaactgga
     9901 agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat tcattgagca
     9961 ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt gtgatgaagg
    10021 gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag aaaaaatggt
    10081 ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa
    10141 agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt
    10201 agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga
    10261 cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc
    10321 aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat
    10381 cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg
    10441 tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca
    10501 tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag
    10561 taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc
    10621 tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca
    10681 atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt
    10741 acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg
    10801 ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata
    10861 acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat
    10921 cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt
    10981 atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg
    11041 atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat
    11101 atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa
    11161 aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac
    11221 gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga
    11281 aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca
    11341 gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct
    11401 ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg
    11461 acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg
    11521 ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg
    11581 gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct
    11641 tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt
    11701 ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc
    11761 agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc
    11821 gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa
    11881 accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga
    11941 tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt
    12001 tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa
    12061 gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt
    12121 gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag
    12181 agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag
    12241 ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt
    12301 acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt
    12361 tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg
    12421 gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt
    12481 gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct
    12541 ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc
    12601 atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg
    12661 gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa
    12721 caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga
    12781 caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt
    12841 atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat
    12901 aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc
    12961 ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt
    13021 ttattttata agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt
    13081 tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg
    13141 aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc
    13201 tgatttactg agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga
    13261 agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg
    13321 atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt
    13381 agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga
    13441 agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac
    13501 aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa
    13561 ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt
    13621 accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg
    13681 accaaattct caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga
    13741 aggaatttac gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag
    13801 ctacttttgg aacaccgtgt acc
//
LOCUS       DAZUMI010000081        13808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000081 DAZUMI010000000
VERSION     DAZUMI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.082"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG81C_001998"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG81C_001998"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5065145.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG81C_001999"
     CDS             complement(2261..2512)
                     /locus_tag="ACG81C_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5065146.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACG81C_002000"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACG81C_002000"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5065147.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACG81C_002001"
     CDS             3462..3680
                     /locus_tag="ACG81C_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065148.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ACG81C_002002"
     CDS             complement(3969..4481)
                     /locus_tag="ACG81C_002002"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5065149.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACG81C_002003"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACG81C_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5065150.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ACG81C_002004"
     CDS             complement(6934..7377)
                     /locus_tag="ACG81C_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5065151.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ACG81C_002005"
     CDS             complement(7378..8166)
                     /locus_tag="ACG81C_002005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5065152.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACG81C_002006"
     CDS             complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACG81C_002006"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5065153.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ACG81C_002007"
     CDS             complement(9281..9868)
                     /locus_tag="ACG81C_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5065154.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACG81C_002008"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACG81C_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5065155.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ACG81C_002009"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ACG81C_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5065156.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ACG81C_002010"
     CDS             complement(12785..13720)
                     /locus_tag="ACG81C_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5065157.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAZUMI010000082        13800 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000082 DAZUMI010000000
VERSION     DAZUMI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.083"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..253
                     /locus_tag="ACG81C_002011"
     CDS             <1..253
                     /locus_tag="ACG81C_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5065158.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            409..2607
                     /locus_tag="ACG81C_002012"
     CDS             409..2607
                     /locus_tag="ACG81C_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065159.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(3189..4061)
                     /locus_tag="ACG81C_002013"
     CDS             complement(3189..4061)
                     /locus_tag="ACG81C_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065160.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            4361..5335
                     /locus_tag="ACG81C_002014"
     CDS             4361..5335
                     /locus_tag="ACG81C_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065161.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            5357..6049
                     /locus_tag="ACG81C_002015"
     CDS             5357..6049
                     /locus_tag="ACG81C_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5065162.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            6015..7523
                     /locus_tag="ACG81C_002016"
     CDS             6015..7523
                     /locus_tag="ACG81C_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5065163.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7597..8700
                     /locus_tag="ACG81C_002017"
     CDS             7597..8700
                     /locus_tag="ACG81C_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5065164.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8703..10040
                     /locus_tag="ACG81C_002018"
     CDS             8703..10040
                     /locus_tag="ACG81C_002018"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5065165.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            10027..10905
                     /locus_tag="ACG81C_002019"
     CDS             10027..10905
                     /locus_tag="ACG81C_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5065166.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10902..11825
                     /locus_tag="ACG81C_002020"
     CDS             10902..11825
                     /locus_tag="ACG81C_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5065167.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11822..12232
                     /locus_tag="ACG81C_002021"
     CDS             11822..12232
                     /locus_tag="ACG81C_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5065168.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            12236..>13800
                     /locus_tag="ACG81C_002022"
     CDS             12236..>13800
                     /locus_tag="ACG81C_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5065169.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4609 a         2295 c         2909 g         3987 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct taattagatg agtatcaatt
      361 ttacagaaaa atgaaagcct tttattttaa aaggaggaga ggaagaaaat gaagaaaagt
      421 acagtatttt ataaaatatt tttaccgatg tttttattag gagccgtact tgtcatagga
      481 tttagtttat tcatttacca gaatacctat gaatcgatcg aatcgagtta tttaacagac
      541 aaaaagaatt tgctcaggca agttaaaact aacgttgaat ggaaaattag aacgattgaa
      601 tattcgtttt ctacgtatgg ttcgaccaaa aatttctcgg acatattcaa gcatccgctg
      661 acatatacag attactccac ttattctgaa atcaggaaag aactaaattt tatagaaacg
      721 attgttatgg atgataataa ttatgattta gtcagtttgg ctggtaaatg gggagtaatc
      781 aatggatcgt tgagtcaact gaccgaagag gaagttgaaa agtatcaaca gaagtacata
      841 aataacaaaa ataatttatt ttggcaaaag aaaaacaaag gaatcgaaat ggtgatttct
      901 ttgccaatgt ttgaaaaaga aaaatatgca ttgggaattg ctacgataga gaaacataca
      961 atagaacaag ttgttggtga aagtggagaa gacatacttt ctattgaaaa cgaagttggc
     1021 aaccttttta ctactcagac agtaaaaaat aaagagcaac caactgtatt aacggacgtg
     1081 gatctagatt acaatcagct gaaagtgata agagaaaaag ataaaacgta tatagtactg
     1141 aaatcagatt ataataattg gatttacaga ttagaagttg actctagtgc aattggcgca
     1201 atgatccgca atctgagaat cgggctagtt gcagtgtctt taacactggt aggactcatt
     1261 ggtctttttt cctacttatt ttcggacaga tttgcccgtc cgatttctca aatacaggaa
     1321 cgtctgaatc ttaaatcaaa aggattcagt gggaaagaac tagatttggt cgctcaatca
     1381 gtcagtcgga tcataggaga aaaagaagcg ttaagtgcac acttagttac ccaaaagcca
     1441 cagctggaaa cgctatttgt acttagttta tttagaaatc gagtagaaag gcgagaacta
     1501 gatcagcggt tgcagcaatt tggttatagt tctcaaaatg actgctatta tacagctttg
     1561 gttcaaatcg atctgttaga agatagtcac ggtggggagc gtgatttgct acttttagcg
     1621 atcaacaaca tgatcacgga aattgtgcca aaggaaaatc gaatgctgcc gatcgttttg
     1681 aatgaagaaa tgcaagccac tatttatcga atggataaag atgatgcaga agcaagcaaa
     1741 aaagtcatgg agtattgccg actcattcaa aaacgtgtaa aagaatactt gaatttaact
     1801 atcagtatag gcatcagtaa ccggttcgaa acgctgaatg aaagcaagca atcggttgat
     1861 cgggcaaaag aagccctgta ttatcgtgta aatactggtc caagttcaat tattttttat
     1921 caggaaatcg ttcctgctgc tcatgaaaaa acacttatta gatatccgat agaagaacaa
     1981 aatcgattgt ttgaagccat tcgttctgga aatcaggaag tgaagaattt agtacatgaa
     2041 ttgattgatg cattgtttgc acaaaataaa aatcctttga gtcgggaagt agtttcggtc
     2101 cgcttgatca atgaactcgt tcaattagga cagttattgg gagtagattc aaaaaacttt
     2161 gataacatga aacaaattta tgtaaaagca ctgaacaatt atcatcccaa agaactagaa
     2221 aaacttgttt tagaacaatt agttctgcca atcacagaat gctctcaagc aatcactgat
     2281 catgaattta aaaatttgtc ggagaatatt atgcatatcg tctacacaga atatgatcgt
     2341 gatctgtctt tagatgtgat cgctgatagg ttgcattata accctaacta tttaagtaat
     2401 gtatttaaaa aggaaacagg agaaaaattt ggtgattttg ttcaaaatca tcgtttagag
     2461 gtagcaaaaa aatggttgaa agagactaac ctctccgtaa aagagattgc agaaagattg
     2521 caatatcgaa atccgcaaaa ttttattcgt ttctttaaaa agaaggaatc gattacgccg
     2581 ggagaatatc gaaaaaaata tttgtgaatc aagaggctgt ttttcttgtg atctgattca
     2641 agcaataaaa gaggacagca aaaaacagca cttcatgttt tgctgctggc tgattttatt
     2701 gccgcagaat caacaagata aacgccgttt aatcaagagg ctgtttttct tgtggactga
     2761 ttcaagcaat aaaagaagtt agaataaccc tagtatttgt atttatggat agttaaattt
     2821 caaggctaag cacgcaaaaa gaactgtgac aaaagtcttg tcacagttct tttttacgaa
     2881 taaatggcgt tcaagagaca ggctatcgac aataaggcat aattttgtga aatatgggaa
     2941 gctatttttc aaaatcgctt cttattgctc ctgtgggtta ctcgtcgcaa tgtttgtgag
     3001 agtttttatc atcgctcaag gctgaacgtc tctttcacaa cctctgatac acggtgttcc
     3061 aggagtagat tcttcgaaaa ataagccgaa atttcacaaa aatttaagaa gcaattttcg
     3121 taaatgcctt cttatttctt gaagctgacc acttctgaca caactaccat tttgcgttca
     3181 gtacataatc accctttgac agaacctacg agtgctcctt tgacaaaata tttctgaaca
     3241 aaaggataaa caatcagcat aggcactgtt gcaataacga taacagccat tttaactgtt
     3301 tgtgctggtg gaatcacatc aacggcacta ctttctgctt gcatcccact tgatacaatg
     3361 acgatttggc gtaaaagaat ctgtatcggc catttagatg aatcgttgat atacaaaata
     3421 gcagtcatat aagtgttcca gtaagtaaca gcataaaaaa gcgagatagt agcaatagac
     3481 ggtaaagcta atggcagcat aattttgaaa aatacaccaa aatctgtgca accatccatt
     3541 tttgctgatt cttccaaact atccggcaag gcttggaaaa agtttctcat aatgatcata
     3601 ttcattgcat taacagccac aggcaggatc atggcccagt aagaattgac cagccctaga
     3661 gaacgaacaa ctaagaacgt tggaatcatc cccccgctga acaacataga aaatacaaca
     3721 agaaagttga cgaaacctcg accaaacaaa tactttctgg aaagaccgta ggccatcagt
     3781 gaagttgcac acatactgac gatcgttccg acacctgtaa caccaatcga aacagcaagt
     3841 gctctaaaaa tggtcggcgt agaaagaatg tagcgatacg cgtctaaaga aaaggtagta
     3901 ggaaaaagga taaacttttt cgcgacgact tcttgtgtag aagcaaaaga tgtagcgatg
     3961 atatttaaaa aaggaacgat acagatcaaa gctagcaaaa ttagaaacgt attgttgaaa
     4021 agtgtaaaac ttctttctcc tatagagact ttctttttca tatagatagg ctcctcctca
     4081 aaataaatta aatgtttctg tttctatatg ttcaatttat catcggtgaa agattacgta
     4141 tataatcatt tcctatgcta agtgtcagat agaaaaaaat agaaggtttg ctatgcgtag
     4201 tttatttgtg aaaaaataca ctttatcgtt ttgtatagca caaaaatcat tgctatgtaa
     4261 gcgtttttac ttacggtaaa aatgattata tacgtaattc agatagttat ctatcatgaa
     4321 gtcataagca gcaaacattc acaaaactaa ggaggggctt atggaaagtg taaagaaaca
     4381 agaaccattt caactgacgc tatcgaaaaa agaagaaaag ctcagaagac gaaaaaaaat
     4441 aattgcagga atcaaaacga ataaattttt atatgtgatg attctgccgg gagtccttta
     4501 tttcttgatt tttaggtatc ttcctatggg gggactcgtt attgcttttc aagattatca
     4561 accattttta ggaataatgg gaagtccttg ggtcggtttc aaacatttca ttcgtttgtt
     4621 tacagaaccg acattcttta cattattaag aaatacgttg atcttgttcg gattaaatat
     4681 cttcgtgttt tttccgatgc caatcatttt gtctttacta ttaaatgaag taacgaacaa
     4741 acgattgaaa aacgggattc aaacgatcat ttacatccct cacttcatgt cttgggtaat
     4801 cattgtttcg attacttatg tatttttgaa tgttgacgga ggggtattga atgaattcct
     4861 tgcaacaatg ggatttgaaa aaatcagttt tctaacatcc cctgaatgga cacgcacagt
     4921 atatatcgga caagtgatct ggaaagagtt gggctggtct acgatcatct acctttcagc
     4981 gataactgca gtcgatacac agttgtatga agctgcagaa atggatggag caggacgctt
     5041 acgcaaaaca tggcatgtta cattgccggc aattcgtcca gtaatcatta ccttgctgat
     5101 tctaaaaatc ggtcagacat tagacttagg gttcgagcat atgtacttgt tattgaattc
     5161 attgaatcga agcgttgcag aaattttcga cacctatatt tatacagctg gattgaagaa
     5221 tggacagtta agtttcagta cagcaatggg attgtttaaa ggggtcgtag gattgtttat
     5281 ggtaatttta gctaataaga ttgccaaaaa gctaggagaa gacggcgttt actaagatag
     5341 aaagagaggg aaagatatgc tgcagtcatt agataaaata aacggaaggc taaatcgtat
     5401 tttacagttg gtttatttga attttctgtg gggcggttta gtcatcgcag gtttgggagt
     5461 ttttactttc ggtcctgcat cgtatgcgat ggttgcagtg attagaaaat ggctgcgctc
     5521 aaaagaaacg tttcctttga cgaaaacgta cttcacatat tttaaagaaa actatcgtga
     5581 aaccttgctg atgagctgga tatatggcgc catcgggatt gttttacttg tggatctttc
     5641 tttcatacac aattggtatc ttaggatcgc tttgtatatt gctgcgttca tatatgtgct
     5701 ctcagcactc tatattttcc caatcatggc tcattatgat tggaaaggat attttttcaa
     5761 aatgaaaatg gcatttttat tcggattctc acatcttcat tactcattag tgttgatatt
     5821 aagtatggta gggatttatc tgatgatcat ccaaacaatg ccaggtgttt taacctttat
     5881 cggtggcagt ctcttctttt ccgcagtcac gtggacagca aattctatat tttgccattt
     5941 ggaatggaag aactcatata cagaaaaaac agaagaaaac aaaaatatta ttaaaacagt
     6001 gaaggagaaa atttatgaaa aaagcaacgc tcgtaaaatt aggtcttagt tttgcaggaa
     6061 ttctagcact tgccgcatgc ggcagcagtt caagtaacag ttcttcagag acagaagatg
     6121 gaaaagctaa aatcacttgg ttaaatattt tgcatcatgc ttcaccgcct acagacacga
     6181 ttgtgaactt gatcgaagaa aaaaccgata cggaactgac attcaactgg attcctgatg
     6241 cttcaaaaga agaaagatta acaactgcat tggcttctga tgaactagga gatatcgtta
     6301 cacttcaaat tgcgatgttg aaaaactctt ctgtccgcaa tgcgttaaaa tcaggcgtat
     6361 tctgggatgt atcagaatat ttaaaagact ataaaaattt aagtcaaatt tctgaagata
     6421 gaatcgaagc tgcgagtatc gacggtgcct tgtacggcgt accgatacaa aaagactacg
     6481 ctcgtgctgg cttagtcatt cgaaaagact ggttggataa tctaggacta gatgttccaa
     6541 ctaccatgga tgagctttat gaagtagcta aacaatttac agaaaacgat cctgatggaa
     6601 atggtgtgga cgacacagtt ggttttggcg atcgtgcgcc tagtgatatt cgttatacca
     6661 gctttaaact tttcacttct tattttggag caccaaatgg ctgggctgta gacgataatg
     6721 gcaaattcac tccagaattt gatacagaag agtatatgaa agctttgaat ttttcaagag
     6781 atctatttga gaatggctat ttggcacaag actttgcagt tacccaaaag actgatcagc
     6841 aagaacaatt tgcacaagga agaactggaa tctacactgg aatggtggat atcaagaatc
     6901 tgaagacgat gggtcaaggt attcaagatg atatggaatt agtgcctgta aacaagatct
     6961 cagatggtga aggcggcggt tatcatgttt ggtcagaagg aaatggtatt ggcggcttgc
     7021 tagcatttcc caaatctcaa gtaaaagacg aagcacaatt gaagaaatta cttcaatttg
     7081 ttgatgatct tttagaagaa gatgtgtata aattgatgac tggcggtatc gaaggaacgc
     7141 attatgaatt gaatgatgat ggttctatcg aatttatcga tatggatctt tggcaacaag
     7201 aggtgcaacc gttatcaagc tctcgtccta gtgaagtgac gttctctttc aaagatacag
     7261 accctgaaaa agaattctct aatcaattga tcaaagaaaa tgaagaattt gctgtaatgg
     7321 atccttctgt gccattggat tcaccgacga acaatgaagt aggatcagaa attgaaaaaa
     7381 tcgtcattga tgctacggtg caatacatca tgggacagct tgacgaagat ggattcaaaa
     7441 aagctgtaga agactggaaa gcacaaggag gcgatcgaat cacacaagag tacgaggaag
     7501 catacaaagc agcggcacag taatatagac gattgggaca aatacttctt ggtatttgtc
     7561 ccaaatccta ttttaatttt taaaggagag caaataatgg aaaactggtc aaagaaatta
     7621 gcaaaaagta tcatggaaag aacacctcgc ttatatgaag aaaagtggta taaaggaaaa
     7681 tggtcttatg actatggcgt cgtacttaaa gggttccaat tactttggga acgaacccaa
     7741 gaaaaaattt attttgattt cataaaagac aatatcgact atttcgtgca agaagatgga
     7801 acgatccgtg gctacagcgt tgaagaatac aatatcgacc atgtaaatac aggaaagctt
     7861 ttctttttat tgtacaaaga aacaggcgaa gaaaaatata aaaaagcagc agaactcctt
     7921 tcccagcagt tggcaaacca tccacgcaca tccgaaggag ctttttggca taaagaaatt
     7981 tatccttatc aaatttggtt ggatggctta tatatgggtt ctccttttta tgctgaattt
     8041 atccagaatt ttagtgaaga aaaagattac caagatgttt tgagacagtt tgaaatcagt
     8101 tataaacatc tattcgatcc aaaaacaaat ttgctgattc atgcatggga tgaaaagaag
     8161 gtccaaccat gggctgatcc aaaaacaggt ctatcccatc atttttggag ccgatcgatt
     8221 ggctggtatt tgatggctgc agcagatacc tatgaattac ttcatgaaga aacagattgt
     8281 tctttgctga aagaaattct agaaaagaca ctggctgcat tacttccata tcaagaagaa
     8341 aacagcggga catggtatca agtaacaaca gaaacaaaac ggaaaggaaa ttatctggaa
     8401 gcttccggca gtagtatgtt tgtttacgca atggctaaag gaatacgttt gggactgctg
     8461 aacaaagaag aatggcttcc acaagtgaag aaatcacatc aaggattact tgatgaattt
     8521 gttctggaaa caaaagaagg ctggctgaac ttgaataaaa actgtcaagt agcagggtta
     8581 ggcggagcag atcagcgaga tggttcatat gcttactaca tcagtgaacc aattatttgt
     8641 aatgatcaaa aaggcgttgg tgcattcttg caagcattga ttgaagtgga ggcattataa
     8701 atatgtatga tattttgaca tttggcgcat caattgatga attgaatact gaagcaatac
     8761 aacaagccat tgatgctgca gcaagtgacg gaggcgggac cgtagttgtt ccagcaggtg
     8821 aatttctaac aggagcttta tttttaaaaa gcaatgtcga acttcattta agcgcaggag
     8881 cggttctgaa attctctgac gatccaaaag attatcctgt tgttcattca cgttgggaag
     8941 gagtccatcg aaaagtttat gcttcctgta tctatgctca aaatgtagag aacatctctg
     9001 tcactggttt cggtacatta gatggaaatg gaaaaaaatg gtggcatact tttagaaatg
     9061 aaccagataa tctcgcttat cctcgtccta aattgatgag tttccataac tgtcatcgaa
     9121 ttactgtgaa agatattaaa ttgatccagt caccaagttg gacgatcaac ccgattcttt
     9181 gcagtaatgc aacgtttgat aacttgacga tcttgaaccc agcggattca ccaaatacag
     9241 acggaattga tccagaatca tgcaaaaatg tgcgtattag taattgccat attgacgtgg
     9301 gagatgactg tattgcaatc aaggcaggga ctgaagatac gtacgaaagg atcgcttgcg
     9361 aaaatatcac gatcacaaat tgcacgatgg tccatggaca tggcggtgtc gtattaggaa
     9421 gtgaaatgag tggtagtatt cgtaatatta ccatttcaaa ttgtatcttc caagaaactg
     9481 atcgaggaat acgattgaaa tctagacgag gtcgaggcgg gatcgttgaa gatattcgag
     9541 tatccaatat agtcatggat aatgtgatgt gtccatttat actgaatctt tattatttct
     9601 gtggtccgcg agggaaagaa ccgtatgtat gggaaaaaaa ggcttatccg atagatgaac
     9661 ggacaccagc attcagaagg atccattttt cgaatatcac ggcaaggaat gtccatgctt
     9721 ctgcaggttt tatttatgga ttagcggaac aattcataca agaaatcaca tttgacaata
     9781 tcgatgtctc gatggcccaa gatgcagttc ccggtgttcc tgctatgatg acaggaatag
     9841 aggaaatgag cagacaagga ttttatattg gctttgcggg taaggtttcc tttaaccaga
     9901 tgtcgattga aaatcacgag ggaccagcat ttctattaga aaatactaaa gaaatcagta
     9961 ttgagaaatt ccaatcaaat caatcagaaa atatggatga actggttgtt cgtaaaaact
    10021 ttgcttatgc agttgaatga ttttttaggt gctccatcta gcagacatac ccctagtgga
    10081 aaacgattat accgagaaaa acaaaacggt tttatgcttg aatactatca attgtactta
    10141 aatgaattgg aaacagtacc agccctctat gcttatccag aaaaagaagg accgtttccg
    10201 ccggtccttt acctgcattc tcacggcgga gatttttcag tcgggaaaag tgaattgatc
    10261 catggtgctc cttacttagc ttctccatct tttgcagaag tactgacagg aatgggatat
    10321 gctgtttggt cgattgatgc ttggggattt gaagaacgcg gaggtatttc tgaaagcgag
    10381 ttgttcaaac aatttttatt aacaggaaaa acgttatggg gaatgcgtat ttatgatgtg
    10441 atcagcttga tcgattattt ggagacaagg gaagacacag atactcaacg gctcgcaacg
    10501 attggtcttt ctatgggagg cttattaagc tggtgggtga cagcattgga ttctcgtgtc
    10561 aaagtttgta ttgatctagc ggcacaggtg gacatcgaaa tacttttgct acatcgccgc
    10621 ctagatcatc atgggtttta ttactatgtg ccaaatctat tgacagctta tacaacgtta
    10681 gcaatccaag aaaaaatcgc tccgcgccct cgtcttagct tagttggaaa aaacgatacg
    10741 atgtgcttgg acgaaggcgt actgaagctg agaaaagggc tggacaaaaa gtatcaatca
    10801 atgggtagtc cagaaaactt ttcttctttt tctttaacag gcggtcatat ggaaacacaa
    10861 gaaatgagaa atatttggaa acaattcata agggagcatt tatgaggatg aaagacaaac
    10921 tgataattgg aaagaatgct atagataccc cgacggattt tgaaactatc caagaaggaa
    10981 tcgattattt ggcaggcctg ccggatagcc atccaaaggt cttgatagta aaagaaggaa
    11041 gatatgatga atatatagaa agcagattat ccaattttaa acttgtggga gcaggaacag
    11101 tagttatctc cggtaatcgt tttgcaaaac aggagtatct tgacggtagc cagttaggga
    11161 ctttccgaag tgctactttt ttcttagaag gatcgaatgt cgtattagaa aatcttcaca
    11221 tcgaaaatac agcaggtcca ggagaaaagg taggacaggc tgttgcttta ttcaattcag
    11281 gagatcgcgt atctttaaaa aactgtcgac tatctggtta tcaggatacc ttatgcacgg
    11341 gtccgttgcc tgatttgcaa aaagatggta ctcctttttg tacaccggct tctgccaatc
    11401 ctgagtattg tcgtcagacc tatgaatatt gctggattga aggaacggtg gatttcatat
    11461 ttggtggggc agatgccgtt tttcatcagt gtcatatcca tagcttagcc agtaaaaatc
    11521 ctggatacat cacagcagca tctacgccga aaaatcaaaa aacagggtat cgttttgaaa
    11581 gctgtacctt gactgcagaa cctgatacta gaaacgtcta tttaggaaga ccgtggcgtc
    11641 cgtatgcaaa tgtcttattt gaagcgtgta agataggtgg gcatgttcac ccaactggtt
    11701 gggataattg ggatgacaag aaaaatgaac agactgtcaa attctctgaa cgtaataaca
    11761 attatgaaac atcgatcaaa agacctgaat ggatacattt agaaaagcag ggggttcaga
    11821 aatgaaaaga aaatgggaac tattgctagg aatggtcggt ggcagtctat ctttgatttt
    11881 ctttggcggg ttagcagtta ctttaagtaa tatgtctgcg tcagagttta aaaaaagtta
    11941 tcagtcgctt gctgtagatc attcaacttt gtctttagaa aatacatttg gattattaca
    12001 ggacatgact ggtctgtttg ccgttgtgtt gtttatttca cttgcttttt tagcagtagc
    12061 attgtttttg actgctaaag gaaaatactt aacaactgcc actggtttgt attttataac
    12121 aggattcata ttgttgattg gcactcagtt tattgcgttt ccttttgcat ttttttattt
    12181 cgcagctggg gcattcagtt tatatcgagt aagaatgaga aaaggagcct gaatcatgca
    12241 gactatcaaa gtgaaaaatc ctattttaag aggattcaat ccagatccta atatattaag
    12301 agtgaaagag cggtattaca ttattgtctc ctcttttgaa tggcttccag gaatccgagt
    12361 atatgagtcg aaaaatttag tcaactggtc acatacaacg gatattttga ccactcaagt
    12421 atctttgcaa ggaaatccta caaatggaag tatatgggcg ccacaaatca gttatgctga
    12481 tggattgttt tatcttgttt acacagatgt caaaagtgca ggccgtccgt ttaaagatgt
    12541 ccacaattat ttgatcactg catctgagat cacaggtcct tggtccgaac caatttattt
    12601 aaatagtaac ggctttgatc cttctttgtt ccatgatctt gaaacgggaa aaaaatggct
    12661 tgtcaatgaa ctatgggatt atcgtctgga cacaccgaat aaatccagcg gtatcgtatt
    12721 acaagaatat gatgcagaga agaagaagct tgatggtccc atctataaaa tatttgcagg
    12781 tacagaatta gcgaaaacgg aggcaccaca tttgtatttt cgtaacgggt attattattt
    12841 gatgacggca gaaggcggta caggaagagg acattcagtt acagtttgcc gagcaaagaa
    12901 tattactggt ccttacgagc ttgatcctgg ttttcctatg ctgacagcta gtgacaaacc
    12961 tgcttctaca ttgcaatgta caggacatgg gagcttagta caaaccccgt caggaagatg
    13021 gtacatgaca tatctttgta ccagaccatt aaacggtgcg gctattttgg gaagagaaac
    13081 cgctattcaa gaagtgtatt ggacgtccga cgattggtta agacttaaag atagccagac
    13141 aactccgatg gaagaaataa tgattgagac aaatgaagag gtacaacaag aaaaagatca
    13201 tcagtttatg gatacatttg aaggagagct aaagaagaac tggaatgcca gacgcatttt
    13261 gccaaatgct gattggtgtg atacaaaatg tagaccaggt tttttacgtc tgatttccgg
    13321 tgaatctctt caatctacct tcgaacatca tttgttagct attcggcaaa cggattttac
    13381 ttttgatgcg gagacagcat ttgaatacat gcctaataat tttaatcaga tggctggact
    13441 ggtactgtac ttaaatgaat ccaattatat ttattgttat gttacttgga atgaggacaa
    13501 gggaaaatgc ctacgtatga tagaatcaga aaacggagta gcacaaatag aagattggac
    13561 gattccactc acagaaaaga agactttttt gaaagtcact gtaaagaaag agacagcaca
    13621 gtttcatttg aaagacgtat caactgaaga atggaagaaa gtaggtccgc aaaaaaacat
    13681 gtgtatactc tcaggagggt ttactggaaa ttttatcggg atagcagtgc atgatctaaa
    13741 taaaaaaaga ggaagttatg ctgattttga gtttttcaag tatgatggaa aagatcatct
//
LOCUS       DAZUMI010000083        13732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000083 DAZUMI010000000
VERSION     DAZUMI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.084"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACG81C_002023"
     CDS             complement(143..1345)
                     /locus_tag="ACG81C_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5065170.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACG81C_002024"
     CDS             complement(1348..1542)
                     /locus_tag="ACG81C_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5065171.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACG81C_002025"
     CDS             complement(1589..2437)
                     /locus_tag="ACG81C_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS5065172.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACG81C_002026"
     CDS             complement(2642..2992)
                     /locus_tag="ACG81C_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065173.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACG81C_002027"
     CDS             3170..4033
                     /locus_tag="ACG81C_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5065174.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACG81C_002028"
     CDS             4657..5649
                     /locus_tag="ACG81C_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065175.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACG81C_002029"
     CDS             5665..6549
                     /locus_tag="ACG81C_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065176.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACG81C_002030"
     CDS             6598..8205
                     /locus_tag="ACG81C_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5065177.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACG81C_002031"
     CDS             8206..9690
                     /locus_tag="ACG81C_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5065178.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACG81C_002032"
     CDS             9867..10859
                     /locus_tag="ACG81C_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065179.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACG81C_002033"
     CDS             10911..11780
                     /locus_tag="ACG81C_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5065180.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG81C_002034"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG81C_002034"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5065181.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAZUMI010000084        13633 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000084 DAZUMI010000000
VERSION     DAZUMI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.085"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..251
                     /gene="yaaA"
                     /locus_tag="ACG81C_002035"
     CDS             9..251
                     /gene="yaaA"
                     /locus_tag="ACG81C_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5065182.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            238..1362
                     /gene="recF"
                     /locus_tag="ACG81C_002036"
     CDS             238..1362
                     /gene="recF"
                     /locus_tag="ACG81C_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5065183.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1359..3305
                     /gene="gyrB"
                     /locus_tag="ACG81C_002037"
     CDS             1359..3305
                     /gene="gyrB"
                     /locus_tag="ACG81C_002037"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5065184.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3325..5796
                     /gene="gyrA"
                     /locus_tag="ACG81C_002038"
     CDS             3325..5796
                     /gene="gyrA"
                     /locus_tag="ACG81C_002038"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5065185.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6032..6331
                     /gene="rpsF"
                     /locus_tag="ACG81C_002039"
     CDS             6032..6331
                     /gene="rpsF"
                     /locus_tag="ACG81C_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5065186.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6381..6893
                     /gene="ssb"
                     /locus_tag="ACG81C_002040"
     CDS             6381..6893
                     /gene="ssb"
                     /locus_tag="ACG81C_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5065187.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6919..7155
                     /gene="rpsR"
                     /locus_tag="ACG81C_002041"
     CDS             6919..7155
                     /gene="rpsR"
                     /locus_tag="ACG81C_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5065188.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7298..9271
                     /locus_tag="ACG81C_002042"
     CDS             7298..9271
                     /locus_tag="ACG81C_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5065189.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9277..9729
                     /gene="rplI"
                     /locus_tag="ACG81C_002043"
     CDS             9277..9729
                     /gene="rplI"
                     /locus_tag="ACG81C_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5065190.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10003..11370
                     /gene="dnaB"
                     /locus_tag="ACG81C_002044"
     CDS             10003..11370
                     /gene="dnaB"
                     /locus_tag="ACG81C_002044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5065191.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12150..13442
                     /locus_tag="ACG81C_002045"
     CDS             12150..13442
                     /locus_tag="ACG81C_002045"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5065192.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4533 a         2471 c         3005 g         3624 t
ORIGIN      
        1 gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt
       61 tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca
      121 ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga
      181 tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg
      241 aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt
      301 tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa
      361 agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt
      421 ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt
      481 ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacaa
      541 aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt
      601 tctttagtaa aaggcagccc gcagatcagg cggaagtttt tagatatgga attaggccag
      661 atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac
      721 cagtatttaa agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg
      781 tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag
      841 cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg
      901 gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa
      961 gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg
     1021 acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact
     1081 tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat
     1141 ttgattaaag aagagacggg ggaatatcca atccttttgt tagatgatgt gatgagcgag
     1201 ctggatgata gtcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc
     1261 ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt
     1321 tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag
     1381 tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg
     1441 ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg
     1501 tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc
     1561 atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg
     1621 tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg
     1681 ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat
     1741 cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg
     1801 tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa
     1861 cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa
     1921 ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg
     1981 ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt
     2041 atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc
     2101 cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat
     2161 atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag
     2221 gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc
     2281 gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag
     2341 gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa
     2401 caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca
     2461 tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag
     2521 cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt
     2581 tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat
     2641 gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc
     2701 gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca
     2761 tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt
     2821 ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg
     2881 ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc
     2941 gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac
     3001 aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag
     3061 tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag
     3121 aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc
     3181 gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc
     3241 gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata
     3301 tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc
     3361 aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca
     3421 cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc
     3481 atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga
     3541 gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc aatggtacgg
     3601 atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct
     3661 gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct
     3721 acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat
     3781 acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca
     3841 acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta
     3901 gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta
     3961 ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg aatccgaaga
     4021 gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg
     4081 ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa
     4141 ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg
     4201 cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc
     4261 gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc
     4321 aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aattcttgaa
     4381 aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga aaaagaaaaa
     4441 gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca tatcgatgaa
     4501 atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac gatgatcgaa
     4561 cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg
     4621 accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa
     4681 gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta
     4741 gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc
     4801 ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac
     4861 aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt
     4921 ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct
     4981 acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac
     5041 gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt actaggtatc
     5101 gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac
     5161 tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct
     5221 aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat
     5281 gtgctactga cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc
     5341 actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt tagaggaata
     5401 cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga tcaagaagta
     5461 ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa
     5521 ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct
     5581 ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt
     5641 atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg
     5701 atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca
     5761 gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat tgtgtaagct
     5821 agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat
     5881 ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat
     5941 aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa
     6001 gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta
     6061 cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc
     6121 aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt
     6181 cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc
     6241 atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat
     6301 tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac
     6361 gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag
     6421 atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga
     6481 atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt
     6541 ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta
     6601 ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag
     6661 aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac
     6721 aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact
     6781 ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc
     6841 cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt
     6901 tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta
     6961 gactacattg ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa
     7021 agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa
     7081 aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc
     7141 gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat cagctattca
     7201 attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta
     7261 tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt
     7321 atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat
     7381 tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg
     7441 ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc
     7501 ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat
     7561 tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa
     7621 agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa
     7681 aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa
     7741 gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa
     7801 cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt
     7861 ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat
     7921 gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca
     7981 gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc
     8041 agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag
     8101 aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg
     8161 aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg
     8221 gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt
     8281 gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat
     8341 ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc
     8401 ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat
     8461 ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa
     8521 aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca
     8581 gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga
     8641 gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag cttcagagct
     8701 agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc
     8761 cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc
     8821 acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca
     8881 atatttattg agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag
     8941 cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag
     9001 tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt
     9061 cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat
     9121 caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac
     9181 tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga
     9241 aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat
     9301 gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat
     9361 tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca
     9421 ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta
     9481 aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac
     9541 agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac
     9601 aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac
     9661 acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta
     9721 caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag
     9781 acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga
     9841 tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta
     9901 taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat
     9961 tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag
    10021 gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt
    10081 gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt
    10141 ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac
    10201 gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta
    10261 agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg
    10321 aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt
    10381 acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt
    10441 atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg
    10501 aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta
    10561 ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc
    10621 attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat
    10681 gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct
    10741 cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt
    10801 cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca
    10861 aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg
    10921 aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt
    10981 gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa
    11041 aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta
    11101 gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa
    11161 caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga
    11221 gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat catcgagaag
    11281 aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt
    11341 tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg
    11401 agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa
    11461 acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg
    11521 gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt
    11581 ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta
    11641 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc
    11701 agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca acctctttct
    11761 taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga aaaatatgag
    11821 aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
    11881 gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc acaaaaattt
    11941 gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc tgttacaacc
    12001 tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt ttataaaata
    12061 aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac aatggttcag
    12121 gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat
    12181 ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct gaagtgattg
    12241 cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt gtgacttata
    12301 aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg atcggaaacg
    12361 gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat gaaaaaggtg
    12421 tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct tatcatatca
    12481 aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca acgattaaag
    12541 gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt gcagacttat
    12601 tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat
    12661 ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa gaatattatg
    12721 aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg aatgatgctt
    12781 tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc
    12841 agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta acaatcggaa
    12901 gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac
    12961 gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa caaatccgcg
    13021 aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt
    13081 ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct ttaaactcga
    13141 tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg
    13201 aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg
    13261 aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca gaactgccag
    13321 aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt atctcaacat
    13381 tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg gcgcaaatct
    13441 aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga agtgtttatc
    13501 tatacgataa taataacttg aagaggtagt gaaagagacg tttagcctta ttgccgatag
    13561 gctgtctctt gaacactgtt tattcagaat aagggactgt gacaaaaacc gtgtcacagt
    13621 ccctttttcc gaa
//
LOCUS       DAZUMI010000085        13213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000085 DAZUMI010000000
VERSION     DAZUMI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.086"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG81C_002046"
     CDS             147..1016
                     /locus_tag="ACG81C_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5065193.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG81C_002047"
     CDS             1225..2250
                     /locus_tag="ACG81C_002047"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5065194.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG81C_002048"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG81C_002048"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5065195.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG81C_002049"
     CDS             3333..5036
                     /locus_tag="ACG81C_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5065196.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG81C_002050"
     CDS             5038..7071
                     /locus_tag="ACG81C_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5065197.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG81C_002051"
     CDS             7082..7522
                     /locus_tag="ACG81C_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065198.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG81C_002052"
     CDS             complement(7722..8849)
                     /locus_tag="ACG81C_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065199.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG81C_002053"
     CDS             complement(8852..9529)
                     /locus_tag="ACG81C_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5065200.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG81C_002054"
     CDS             9793..11559
                     /locus_tag="ACG81C_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5065201.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG81C_002055"
     CDS             11574..12011
                     /locus_tag="ACG81C_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065202.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG81C_002056"
     CDS             12024..13175
                     /locus_tag="ACG81C_002056"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5065203.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2732 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttg
//
LOCUS       DAZUMI010000086        13086 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000086 DAZUMI010000000
VERSION     DAZUMI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.087"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG81C_002057"
     CDS             complement(79..2199)
                     /locus_tag="ACG81C_002057"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5065204.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG81C_002058"
     CDS             2429..3607
                     /locus_tag="ACG81C_002058"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5065205.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG81C_002059"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG81C_002059"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5065206.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG81C_002060"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG81C_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5065207.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG81C_002061"
     CDS             complement(4960..6213)
                     /locus_tag="ACG81C_002061"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5065208.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG81C_002062"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG81C_002062"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5065209.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG81C_002063"
     CDS             7790..9136
                     /locus_tag="ACG81C_002063"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5065210.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG81C_002064"
     CDS             complement(9444..9830)
                     /locus_tag="ACG81C_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5065211.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG81C_002065"
     CDS             complement(10098..10247)
                     /locus_tag="ACG81C_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065212.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG81C_002066"
     CDS             10709..10921
                     /locus_tag="ACG81C_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065213.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG81C_002067"
     CDS             complement(11199..12998)
                     /locus_tag="ACG81C_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5065214.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAZUMI010000087        12974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000087 DAZUMI010000000
VERSION     DAZUMI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.088"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..620)
                     /locus_tag="ACG81C_002068"
     CDS             complement(<1..620)
                     /locus_tag="ACG81C_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFS5065215.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKR"
     gene            complement(625..810)
                     /locus_tag="ACG81C_002069"
     CDS             complement(625..810)
                     /locus_tag="ACG81C_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5065216.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1164..1409)
                     /locus_tag="ACG81C_002070"
     CDS             complement(1164..1409)
                     /locus_tag="ACG81C_002070"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065217.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1403..1804)
                     /locus_tag="ACG81C_002071"
     CDS             complement(1403..1804)
                     /locus_tag="ACG81C_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5065218.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1991..3799)
                     /gene="uidA"
                     /locus_tag="ACG81C_002072"
     CDS             complement(1991..3799)
                     /gene="uidA"
                     /locus_tag="ACG81C_002072"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS5065219.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(3822..5588)
                     /locus_tag="ACG81C_002073"
     CDS             complement(3822..5588)
                     /locus_tag="ACG81C_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS5065220.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(5601..6395)
                     /locus_tag="ACG81C_002074"
     CDS             complement(5601..6395)
                     /locus_tag="ACG81C_002074"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5065221.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(6405..7781)
                     /locus_tag="ACG81C_002075"
     CDS             complement(6405..7781)
                     /locus_tag="ACG81C_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5065222.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(7901..8548)
                     /locus_tag="ACG81C_002076"
     CDS             complement(7901..8548)
                     /locus_tag="ACG81C_002076"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS5065223.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(8632..10059)
                     /gene="uxaC"
                     /locus_tag="ACG81C_002077"
     CDS             complement(8632..10059)
                     /gene="uxaC"
                     /locus_tag="ACG81C_002077"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5065224.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(10133..11212)
                     /gene="uxuA"
                     /locus_tag="ACG81C_002078"
     CDS             complement(10133..11212)
                     /gene="uxuA"
                     /locus_tag="ACG81C_002078"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5065225.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(11215..12846)
                     /locus_tag="ACG81C_002079"
     CDS             complement(11215..12846)
                     /locus_tag="ACG81C_002079"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5065226.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
BASE COUNT         4117 a         2609 c         1658 g         4590 t
ORIGIN      
        1 cgtttataaa attcttcata aaaatccccg tgataaacac tattatatac tttcttaaat
       61 tcatcaactg tccaaaattc tacttgagga cgctgagttt ttattttacc tatagtatct
      121 acaggatttt cttcaataat ttcaagtaca attgcccttt taaaaactac tctcaactta
      181 ttgaatatct gtttaatata atttggtgat aatggttgct caatgccctt ttttacaaca
      241 tggattttcg ataaaaactg ttgaaattcc tgtatatgaa ttggtcgaat ctttacaacc
      301 tgcatttctc caaaaaaatc caatgcacgt tgtaaaatat gatctgtttt catatatgtt
      361 ttttctacag ttcccaactt ataccaagga aaaaaatact ctcgataaaa atcatgaaac
      421 gttattcctt gcagataaat tccttgtgaa tcagaaaaac tttgttttaa ctcttcgagt
      481 ccccttctag cttctccttg agttttgaag cctcttctgg tcacttgaat tctttttcca
      541 aagcgatctt gtcctaaagt tgcagaaaaa aaccaagtgc ctctttttct gtctttataa
      601 acattcgcaa ttttcctcat tttactattc ctcccaagaa atatggagaa cttcttctaa
      661 aattgaaata ggaattcttt ttatttttga attacgataa aaaaagtatc ctttctcaac
      721 taaaagttct ttacattcct tgaagatttt attagcatag tactgagaaa aacctagatt
      781 aatcaaatct tttcgagtag ctgttttcat tatagcacct tccttacttt ttttcattta
      841 tttctatcag ctgcttttgt caaaagcaca taggaatttc acctctcccc aagtttacgg
      901 cgagccttac ggaagtatca ttatcccaac tttaaatcat cgctttttat tgactataaa
      961 aattatttta gaatatttat cgctccttct ttacagcaag aaatcatggc gcaactaaaa
     1021 tatgctcctt aaagtgttaa cgtactgata gaatctattt aattttcaat gaacaatagg
     1081 ttttctacct aaaagaatct actaattcct taactattag aagattcttt taaacagaaa
     1141 aagtggaaag gggatttttt taattagtta attttaaatt ttaagatcgc ttctattaat
     1201 ttaatttcta gctgacttat catatattca tctacatagg taacgaattg acctacctcg
     1261 ttaaatccat ttgtcaaaca taacttagcc atataagaat gataatgttt gagtatcatc
     1321 ttgatcgaaa ttggatctcc ttctagagca gataagataa tggatagtgg aactaatagg
     1381 tactgccgct ttttagattt atctaccata ggtcataccc cattttcaat tgttcaactg
     1441 ccttctttcg ttgataccag ataccacttg tgctcatatt gagaatttta ccgatttcag
     1501 catcattaaa acctgcaaaa taataaagta ggataatctt tcttcttatc tccgataact
     1561 gatcaattgc tgctgataac ttttgatctg aaattaatat ttccattcca aataagaaat
     1621 agacatcttg ttcatcaaaa tgagtttggg aatacctgcc ttcatttgaa tctgccaatg
     1681 gttcctcatt atcctttatg ttagcgtttc gttttcgaat agacttagct tcgtttcgta
     1741 atactttttt acagtaggca tcaaattggt gttgtataga tatgtcattg taatgttgtt
     1801 gcatgataat atgctccttt ttcccccttt ccactaatac aacaatcaga aagccccttt
     1861 ttttcgaaaa aactagaaaa aaaacataaa aatatttatc attctttaaa cagaaaatct
     1921 ttctataaaa aacaaaaaaa aacgattagg ctaggctaat cgttttttga acattcttaa
     1981 aaaatccaaa ttatttttta taactgaaat tgggtattga tgaccatcgt ttctgtagat
     2041 agtaagcaat cattttaggt tttctatctc tagtaaagat tcctttctta tttccttgca
     2101 ctcttaaaat cccttgactt gttgcaaaat ctgcaaaatt ccacacttgt tcaccaacaa
     2161 aattttcaca tgtatctatt acttcgtgat ttgcttcgta ataagccact tgatactctt
     2221 ctgtaaacat tgttggtact gtatcatgaa atccagctac tgtatctgct ccatattctg
     2281 taaacattat tggcgtatca ggacatcttt ccatccatcc atctagctct tttttcaaca
     2341 gatattttgc atgtgccaga tcccctcccg aaacatacca accataataa cgatttaaac
     2401 ataaaacatc gacaatatct cctaccttac aaacatctgg cgttgatttt agataagtaa
     2461 cgattgttac aggtcttttc tgcggatcaa gttctttagt taattcaact aaaggttcaa
     2521 aatattcttt agcaccttcg gaatccgaat ctggttcatt tgcaatagac cacattacaa
     2581 cacttgggtg gttcttgtcg cgttctatca attccttgat caccgtttta tgagaatttt
     2641 ctgttcgtat ctctttccac gtattatgtt tttccacatt atcatctaat aaagtggcca
     2701 tgaaatttaa atgtaaccct actgcaggta cttcatcaat aactacaata ccttctcgat
     2761 ctgctaatcg cataatttct tctgagtaag gataatgtga tgttctaaaa gaattagcac
     2821 ctatccattt cattaaatta aaatctttaa tatttacagc ctcattcaaa ccacgtccat
     2881 taacatacga atcctcatgc ttaccaaatc ctttaaaata aaaaggttta ttattaatca
     2941 aaaatttgcc attggtaacc tgaacactgc gaataccaaa ttcttctgta taggtatcga
     3001 tcactttatt atcatcaatt aaactaattt tcatactata taaataagcg tctagaggtt
     3061 cccaaaaaat tggattctta agctgaatca ttcctttctt tccttcagct tcagctacaa
     3121 gttgagcctc ttcgtcatat atcgctatta agatttttcc ggtccattct gtatcgatat
     3181 tgaattctac acttgcatcc aaatcagtta ttcttgtatt aatagtaata tcacttatat
     3241 agtctttcgg agttgtatat aacttaacag gtctatgaat acctgcataa ttgaagaaat
     3301 caaaattaac taagttttcc ttttttatat ccccattttc ctgtactgtt tccttcataa
     3361 tccccacagg taaagtacta tcgtcaataa tattgtttac agcaactgta attcgattat
     3421 tcccgtcttt tacaaaccga ttaatttcag actcaaatgg tgtaaaccca cctttgtgtt
     3481 gagtaataaa ctttccattc aaaaaaactt ttgcttcatg tgttgcagat ccaaaacgaa
     3541 gaactaatct ttcttctaat aatcttttag gaagagtcac ttctttttca taccacaccc
     3601 atccaacatg atttctaatg ttctctgttt caaataaatc attataagaa gagggaactg
     3661 aaatcggata actctctttt tcatttattg gttctaaagc aagttcttcc tgtaagtcat
     3721 catttgtatc taatttaaat ctccagagac cgcttaagtc aattacttgc ctcgtctctg
     3781 tcataatggg atacaacata taaaccactc ctatctaaat attaaataaa tgtatccttt
     3841 aaaccacaat aagcatctat tggacttact cccttaaatt cagaaagacc cattagtttg
     3901 tccaccaata tttctaaagt ttcttgtttt ccatcataag tattaatata agtctttact
     3961 tgaggtacat cagccaaatg aaacggacac tgtacagata caaaaattgt tggaatttca
     4021 tgaatgtaaa acggtatatc cggagttcct ttacttgctg gccagacaat tcgttgaact
     4081 gtgcctggat taacattagc tatattaatg atcaagtcat attttttagt taaattttta
     4141 attggtctct tagcagcata tattccagct atatttttaa ttttttcttc ttgcgataat
     4201 cctttcaatt cttcttcagg tgaaatgtat ttttcaactt caaatccttt tgctgacaaa
     4261 atctgtttca ataaatcaat ggcttgaggt ctatttccag caactacttc accaaaccca
     4321 ccttttaccc catgtacatc tactagtaaa atccttttat atttttctgg tacaataggc
     4381 caaatatctt gtttatcttt aactaatgta attgcttggt cagccacctc tttaaaaatt
     4441 gatttatttt caattaaacc aattttttcc agagcttctt gcttaggtaa taaaatttgt
     4501 tcttttgctt ttttgtgtaa tcccaattta gcttttaacc ctaaaattct ttttaaagca
     4561 tctaataacc gatcatcagt gataattcct ttacgatagc cttccatcat ataattaaag
     4621 tcttcatctg gatcattaaa aaatagaaat agatcacatc cagctgcaat agaagtgggc
     4681 aataattctg atcgtttcat tgccccagtc attcctaaca tatgactagc atcagtaact
     4741 actaatccat taaatccgag ttttcctcta agtaagtcag tgattaattc ttttgataaa
     4801 gttgcaggaa gtaaatcttc atctttcaga tcaggattaa aatgttttga ataagctggc
     4861 atatggatat gacctgccat aattgaaggt aatccagctt ctattaagcc tgagtatact
     4921 tttccaaaag aattatccca ttcttccgta cctaaactat taatactaaa agatagatgc
     4981 tgatctcgtt cgtcaatacc gtcaccaggg aaatgtttag ctgcaggcaa aattccactc
     5041 tcctgaatac ctttcatata agctaaacta agttctaaaa ctttatcagg gttttgtcca
     5101 aaagatcgac tagaaatgat aggatttctc caatttctat taatatctac aataggagca
     5161 aaactccagt tacatccaat agccgatgct tcaatcccag aaactctccc taattcataa
     5221 gcatatttcg catcatttgt tgcaccaatt tttacttcta caccaacttc agtaccatct
     5281 gtacaagctc cgttaccacc tgcttccgta ttagcagcaa ttaatagagg aattttactt
     5341 tctgtttgta agatatagtt ttgatcatat acctcatcag cagttcctgg attatatcgt
     5401 acagctccaa tatggtactg attgaccata ttactcaagt aatttttatc acgactagat
     5461 cccatattaa caaacaattg acctattttt tcttccaaag tcatagaatc aatcgtctct
     5521 tcaatccaag aaatagcatc ttcatctaaa ttatatggtt tttttcgtaa atctactttt
     5581 atagtcattt tctttctcct ctacttatta gtaagatttt tccaaaaagt atctatatat
     5641 ttttgttcaa tatctacata atctcccgat aaacatttaa ataaaacatc agttcttaag
     5701 ttttcccatg attctttttc tttattgacc aaaataggat aaaagaaaac tttattttta
     5761 tttgcagctt ctagatcacc aggagaatca ccaatcatta atatcttatc caaatcacaa
     5821 ccattttcaa ttacacttcg aataatatct tctttttttc ctttatcttg acaacacagt
     5881 tcatcaacta atgataataa ttcatgtctt tcccattctt cttgaacagc ttccttatta
     5941 gctgagctta caataattat cttcccatat tttttcagag cttttatagt atctctagca
     6001 cttaaaaagg gtttatctaa tccttttgct tgactaatct gttgattgac ttcttccgac
     6061 cattttaaag cttttaataa atcctctgac tgagaatatt ctatttccct cttcaaagat
     6121 ggattagaca attcttttgt tagagatacc cagtgcttta atcgattaat cgatgttaca
     6181 tcattttttt ctaatgtttt aactaaacct aaaaatctgt ttattccacg agtctctgaa
     6241 tataaattaa tattattcca atcctgtagg aatttttctc tatgatgaac atcaaaaatc
     6301 tctgcagcaa ttggtccgaa aaagagattg tgtttataag tcatcgtatc cataacacag
     6361 ccatctgaat caatacaaaa tatccaatta ttcatatttc tctcctattt atgtttttgt
     6421 tttaaaagct cgcaattttc taagacttta tctttagata atggataaag gaaaaataaa
     6481 attaatccta caatcagaaa acaaattgat ggaattaatg ttgaagcgtt atataacttt
     6541 tctaaaacat ctcccgtttg tacatttgct aaagagtcat agcctataat agaaagagtc
     6601 caacctccga cgcctccagc tagagcttga cctatttttc tagcaaaaga atataccgca
     6661 tatacggtcc catcttctct ttgatcagac tttacttctt gatcatcaat aacatcagta
     6721 atatttgccc aaataacagt attaaacaca cccaaaccca tatagccaac cgaactaaga
     6781 attaagaata catacatatt actagctttt atcaaaaata agatcaaata tactattgct
     6841 gaaaagaaca ttcctactga tgccagttct ttttttccat attttttact taaaaacaaa
     6901 gttaaaggcg aaacaactat taaaaccaaa ataggattga taaaattcat taaaactatt
     6961 ccctgtgcat cattatagta atcaggaaat acataattat tcatactcat aattaataat
     7021 tgagcaagga gtaagaaaat agccgcacca attatagaaa gtaatgccct atttttaact
     7081 aaccctttta aagattttaa caatgaaact ttgtattgag attggtcaat tcttactcgt
     7141 tcagtagtta gtttataaca tattaaataa caaataagtg cacatatcga aaatattccc
     7201 gcaacaattg taaaagttga tccaccacga actatttgag cgcctgaatc atctcttgta
     7261 taaataaata atggtgttaa tacaccaatg accaaacttg ctaaagtagc tccaacattt
     7321 cggaatgtcg atagcattgt tctttcttct gtaatagaag taatagctga tgccattgat
     7381 ccataaggaa tatttataga tgtgtaaaaa atactccccc ataatatata cgtaacatac
     7441 atgtatacta ccttaacgct catagaagct gaaatcatag aagtttgata cattaaaaaa
     7501 cttgctaaag cgactggacc actcattctt aaaatccacg gtttaaactt accctgctta
     7561 gttgctttcg atctatcaac aatacgcccc atagttacat cagtaactgc atcgataaat
     7621 cttgctacta aaaataatgt tccaacggca gctccgctta tccccaatac ttttgtataa
     7681 aagatcatta aaaaagaact agcaaaaatg aaagtaaaat cgtttccaaa atctccaaac
     7741 atatatccta ttttgtctct tataccaaat tttctttcca tacaattctc tcccttctgt
     7801 tgttaagcgt tttcattctg atgaaagtac gtactatact ctgaagcgta aagagtcagt
     7861 atttattttt tacatttcag agtattattt gttttttctc ctaataattc tttatctcat
     7921 aaaacttatc tatatattta ctagctactc gacttacttc ttcaaaatca cctttttcaa
     7981 cagaagccaa taaattccca cctacaccaa ctgcaacaac tcctacttca aaccaatctt
     8041 ctaagttctc tatacttact ccgccagtag gcattatatt tacgtgtggt aatggagctt
     8101 gataggcttt tacaatggat ggaccaaaag cagaacctgg gaatagttta atgatatcta
     8161 cgccagcagt aagtgcctcc tttatctcag taattgtcat acaacctggt aaataaggaa
     8221 tttgatataa attacaaatc tcagctgttt gtcgatcaaa agatggacta acgatatatt
     8281 ctgccccagc cataattgcg atacgagcag taaacgcatc taaaaccgta ccggctccga
     8341 ttattacatc tttttgatca gcatattttt tagcaatttc tttgattatt tcttcagcat
     8401 taggaactgt aaaagttagt tcaatccctt taattccacc cgcaataatt gaatttattg
     8461 ctttaatggc ctcatcctta tttttcccac gtacaaccgc aatgactcca gccgcttcta
     8521 acttacttaa aatttctact tttttcatat ctatctcctt cctttatgga tacatacttt
     8581 gtctagctgt actaacttac aaagaataaa tttaagcttt tatttcaagt attattttct
     8641 acttccgatt tcaaaaaaat taaaataatt atacgcatta cgataacaaa tattctccac
     8701 tatctgacta agatacattt catcatctgg aattcggcct gctttaaccc actcgcttag
     8761 taattgacat aatactcgac gaaaatactc atggcgagga tagcttagaa aacttcgaga
     8821 atcagtcaac atacctgtaa aattcgacaa caaactttgt tgtgccatcg tagtcaactg
     8881 ttgacgcatt ccttcagctg tatcattgaa ccaccaagca catcccaatt ggattttttg
     8941 aatcataccc ccttggaagc tttgcattgc tgttgctagt ggaagccaat cagttggatt
     9001 taatgaatat aaaattgttt ttggtaagca cccactcttt tcagcggtat cgaacaatgc
     9061 tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga catctactcc
     9121 aatttgttcc tttaatatac ttgaattatt tcttaaagca tttaaatgca tttgcatcgt
     9181 ccaaccatat cttgtatact cagacatcaa atgtaattga atacctgtaa caaattgtgc
     9241 aatttctgat aaatggaaat ccgtttttcc tgctaaagct tttattaaca gattgtcgag
     9301 tattttttca tctgctattt cataaaaata agtattcaaa ccgtgatctg ctaatcgaca
     9361 gccaatttga tgaaaatgat ccactcgttg gctcaatgct tgcttcaacc cattaaagtc
     9421 tttgattggt atagccgctg tttgactaag ttttaaaata tagttcgaaa agcctgatga
     9481 tttgatactc cagaccttat ctggacggaa agtaggtaat accttgaaag aattatcctg
     9541 tttagaaagc tcagtatgat accacaagtc atctgtagga tcatccgttg tacagatggc
     9601 tttcacatcc atcatctcca acaattcttt cgggcgatag gaatccgttt gaattttttc
     9661 gttagcacgt cgccaaattt ctggtgcatt cttctcagaa attgtcaaat caatatcaaa
     9721 gtaacgttgt aattccaaat gagaccattc aaaaatagga ttacctggcg ccttctccac
     9781 tgtttttaca aaagctaaaa atttttcata atcatcacct ggaccactga tcaactcttc
     9841 ttctatacca ttagctcgca tcaatcgcca tttataatga tctccagctc ctttatcatt
     9901 taaccatacc tgagtcaaat tcttgtattg cttattttct agcaaatatg ctgggtctaa
     9961 atgacaatga tagtcgataa ttggttggtg ttttgcaaat ttatgaaaaa gtcttttagc
    10021 tatttcattc tctaataaaa agttcttatc taaaaacata tttaaacact cctttccttc
    10081 cgttagtttt tagaagagaa aatttttcct ctaaataaac tcccttatat aattatttac
    10141 tcgccccctt ttcaagcgct tcccaaatac catttaaata aactgcacct agagctcggt
    10201 catataatcc ataaccaggt ctaccggttt ctccccaaat catccgacca tgatctggac
    10261 gaatatagcc attaaatcca ttatcgtaca ttgtttttaa tatcttatac atatctagag
    10321 aaccatattc tgatttatgc ggcgcttcgt aaaaatcgag tccattgtca cttaaatatt
    10381 tgatattacg aacatgtgca aaaggaatcc gatcccgttg acaaaattca gatagaattt
    10441 catagacatc attatctggt cgttctgcaa tacttccagt acataatgta ataccattag
    10501 aaggagaatc cactacatta cacaaccaat ctaaatcgtc acgattttta acaatacgag
    10561 gtaatccgaa aatggaatat ggtggatcat ctgggtgaac tgccatttta atcccaactt
    10621 cttcacatgc cgggataatt gctttgagaa aatatgcaaa gttctctcgt aatttctctt
    10681 catcaacttc tttataggct tctaataatt gcttaatttg tgataaccga tgaggttccc
    10741 aacccggcaa ttcaaaatta ttagtagaat tttctacttc tctaataatt tcctccggat
    10801 cagtagggat attttttgaa atataggcta acgtagaaga cccatcatct aaacgataat
    10861 ccaaatccga ttttacccaa tcaaatactg gcataaaatt ataacagata acctcaatcc
    10921 caaattctgc caaattgcga atcgtctgtt tataattctc aatgtattga tctcgtgttg
    10981 gtaaaccaat tttgatatca tcatgaacat taacactctc aataactttc aaggtaagtc
    11041 cattggcttc gacttcatct tttaaagatt taattcgttc tctcggccaa acctctccgg
    11101 ctggtatatc cattaatgtt ccaacaattc ctttcattgc tgggatctga cgaatttctt
    11161 gtagtgagat tttttcttca tttgccccat accatctaaa agtcatttcc atagttactt
    11221 tcctccaact tctgttatat atttattgat tgttttacga actgcttgag gacctactag
    11281 catttcgcaa aagatttttt cagttttctt tcctacccca acttgatata aatctaaacc
    11341 aaagatttct ttattttcta aaatttcctg taccgcacaa tgaatctttg aaggatcttg
    11401 ttcacctaat ttcaatactg ataatttatc ctgcaactct accaataacg gatcaggact
    11461 tggcacaaaa ttttcccctt gatcatcgac tgccagtaaa taacgtaacc aagcagcaat
    11521 agcgatagga ataaatgtta gattttctgc atttccgtct ttttcgacat actttttaat
    11581 tgtttcacca tagcgaatcg ctactttttg agaagtatct gttgcaattc gttgtggtgt
    11641 atctggaata tagggattag gcaaacgttt attaattact tcatcaataa attgttttgg
    11701 atcaataatg cctggatttt caactactgg taaaccttct aaataaccta ttcctttgat
    11761 taaaccaacc aaatcatcat cctgcatctc tgctgaaatt gattgatatc ctagcagaca
    11821 acctgttact gccaaagcag tatgaagtgg attcaagctt gtcgtaacct tcatgatgtc
    11881 tgctttatca accgtctctc tatctgtcat aatcacacca gccttttcca atttaggccg
    11941 gccatttgga aaactatctt caataactaa ataatgaact acctcagtat tagtgaaagc
    12001 agcaatattg gtatgttttt ttgtgtgaat gatatctaaa tcctcgagcc ccatcatcaa
    12061 tagatcttcc tttacattct ctgaaggatt tggcgtaatt cgatcaatca tactccaagg
    12121 aaaagtcact ttttctttat cctttaaata agcaataaat ttttcagaaa catatccttc
    12181 tttttgccaa gcttctgcaa tcgtaaaaat actatcttga aaacgttgac cattctgtga
    12241 gaaattatca gtagaaacca tcgcaatcgg caactctcca gcattaaatc tgctatataa
    12301 caaggatgtt acaatactca tcgtatgttg aggattgcta ggtccagatt tgatatcagc
    12361 tttgactacc gaaaagaatt gccctttaga atcttttaaa ctgtaacctt tttcagtaat
    12421 agtaaacgtt gtaaactgta atttaggatc ttcaaaatat ttaaccactc tttgatagtc
    12481 cgctatattc ttcggattgc aatataaact atcagccgtt actgccaaaa gtcttttttt
    12541 gagttcacca tctccactca taacaatttc caaaatgtca ttgttatagt catgataagc
    12601 tttttccact acttcttcat caaatgtttc acacacaact actcctgttt ctaattcatt
    12661 ttgatctgct aagcattgag caatttctgc atgaaatcct cgataaagat ttcctccacc
    12721 aaaatgaagc catactggat gttgctggcc ttcttgctta acttctttga catcaaaaca
    12781 cggcagtaga atctgcattt cattgaattc ttcgatagaa ttttgccaat ttttaatcga
    12841 aaccataatt aacctcctca aactgatatg ttagttttga tacattttat attttaaaac
    12901 ccattgtaac cgttgtcaat ataaaaaggg aataaacttg cttttttcta ccatatcaga
    12961 atgattgtat tttt
//
LOCUS       DAZUMI010000088        12931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000088 DAZUMI010000000
VERSION     DAZUMI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.089"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG81C_002080"
     CDS             131..3658
                     /locus_tag="ACG81C_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5065227.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG81C_002081"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG81C_002081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5065228.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG81C_002082"
     CDS             7446..7787
                     /locus_tag="ACG81C_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5065229.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG81C_002083"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG81C_002083"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5065230.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG81C_002084"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG81C_002084"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5065231.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG81C_002085"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG81C_002085"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5065232.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4395 a         2247 c         2740 g         3549 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca g
//
LOCUS       DAZUMI010000089        12738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000089 DAZUMI010000000
VERSION     DAZUMI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.090"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACG81C_002086"
     CDS             97..540
                     /locus_tag="ACG81C_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5065233.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACG81C_002087"
     CDS             complement(546..1730)
                     /locus_tag="ACG81C_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5065234.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACG81C_002088"
     CDS             1998..2696
                     /locus_tag="ACG81C_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5065235.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACG81C_002089"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACG81C_002089"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5065236.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="ACG81C_002090"
     CDS             complement(3506..4039)
                     /locus_tag="ACG81C_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065237.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACG81C_002091"
     CDS             complement(4336..6717)
                     /locus_tag="ACG81C_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5065238.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG81C_002092"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG81C_002092"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5065239.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACG81C_002093"
     CDS             7418..7957
                     /locus_tag="ACG81C_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5065240.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG81C_002094"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG81C_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5065241.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACG81C_002095"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG81C_002095"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="ACG81C_002096"
     CDS             9064..10230
                     /locus_tag="ACG81C_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5065242.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="ACG81C_002097"
     CDS             10276..11772
                     /locus_tag="ACG81C_002097"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5065243.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="ACG81C_002098"
     CDS             11853..12536
                     /locus_tag="ACG81C_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5065244.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12545..>12738
                     /locus_tag="ACG81C_002099"
     CDS             12545..>12738
                     /locus_tag="ACG81C_002099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065245.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3605 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DAZUMI010000090        12734 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000090 DAZUMI010000000
VERSION     DAZUMI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.091"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG81C_002100"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG81C_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5065246.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG81C_002101"
     CDS             complement(323..892)
                     /locus_tag="ACG81C_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5065247.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG81C_002102"
     CDS             1078..1980
                     /locus_tag="ACG81C_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5065248.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG81C_002103"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG81C_002103"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5065249.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG81C_002104"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG81C_002104"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5065250.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG81C_002105"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG81C_002105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5065251.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG81C_002106"
     CDS             8548..8733
                     /locus_tag="ACG81C_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5065252.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG81C_002107"
     CDS             complement(8795..9991)
                     /locus_tag="ACG81C_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5065253.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG81C_002108"
     CDS             complement(10161..11597)
                     /locus_tag="ACG81C_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5065254.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACG81C_002109"
     CDS             11890..12699
                     /locus_tag="ACG81C_002109"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5065255.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2673 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgac
//
LOCUS       DAZUMI010000091        12511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000091 DAZUMI010000000
VERSION     DAZUMI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.092"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..438)
                     /locus_tag="ACG81C_002110"
     CDS             complement(76..438)
                     /locus_tag="ACG81C_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065256.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(512..892)
                     /locus_tag="ACG81C_002111"
     CDS             complement(512..892)
                     /locus_tag="ACG81C_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5065257.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            974..2149
                     /locus_tag="ACG81C_002112"
     CDS             974..2149
                     /locus_tag="ACG81C_002112"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5065258.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2226..2810)
                     /locus_tag="ACG81C_002113"
     CDS             complement(2226..2810)
                     /locus_tag="ACG81C_002113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5065259.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3011..4009
                     /locus_tag="ACG81C_002114"
     CDS             3011..4009
                     /locus_tag="ACG81C_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5065260.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4121..4630
                     /locus_tag="ACG81C_002115"
     CDS             4121..4630
                     /locus_tag="ACG81C_002115"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5065261.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4660..5319)
                     /locus_tag="ACG81C_002116"
     CDS             complement(4660..5319)
                     /locus_tag="ACG81C_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5065262.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5321..6085)
                     /locus_tag="ACG81C_002117"
     CDS             complement(5321..6085)
                     /locus_tag="ACG81C_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5065263.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6093..6770)
                     /locus_tag="ACG81C_002118"
     CDS             complement(6093..6770)
                     /locus_tag="ACG81C_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5065264.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6807..8192)
                     /locus_tag="ACG81C_002119"
     CDS             complement(6807..8192)
                     /locus_tag="ACG81C_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5065265.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8402..8486)
                     /gene="ffs"
                     /locus_tag="ACG81C_002120"
     ncRNA           complement(8402..8486)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG81C_002120"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8509..9024)
                     /gene="tadA"
                     /locus_tag="ACG81C_002121"
     CDS             complement(8509..9024)
                     /gene="tadA"
                     /locus_tag="ACG81C_002121"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5065266.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9131..9988
                     /locus_tag="ACG81C_002122"
     CDS             9131..9988
                     /locus_tag="ACG81C_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065267.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10069..10404)
                     /locus_tag="ACG81C_002123"
     CDS             complement(10069..10404)
                     /locus_tag="ACG81C_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065268.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10471..11841)
                     /locus_tag="ACG81C_002124"
     CDS             complement(10471..11841)
                     /locus_tag="ACG81C_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5065269.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11919..12455)
                     /locus_tag="ACG81C_002125"
     CDS             complement(11919..12455)
                     /locus_tag="ACG81C_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5065270.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3714 a         2549 c         2098 g         4150 t
ORIGIN      
        1 tgtatttttt ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt
       61 cgcttaatct gtattttaga agaaaggcac tccctctact tcgtaagttc caagaacaga
      121 gcgcatttga cctggttctt cccaaatata tccttcgcct aattcttctc tttgattctt
      181 ttgttcagtt tcaaagacaa acaacggaac attttcctct ttaatatgtg tatttgtcaa
      241 ttccatcaaa ctaatcttgc taacatcgat atgaactatt tcttttaaga aattaagtat
      301 attcgctaaa ccagtctttc cttcagaaag ttccgctaac gcaaattcca acttgtcatc
      361 aattgaatgg accaaaaatt ttttagtacc atcctccaaa tgcaacataa ttgtaccagc
      421 aactcttttg cttgccaaaa tttccacctc gatcaacttg ttctatggct tacataaaaa
      481 ttctagcaca ttttcagaaa aaatacactt tttatttctt attttctttc cgttctagtt
      541 cagataatgc ttcttctatc caaataggta actgctccgc aatagtggta aatccgatct
      601 tacgattttt attcgtgaaa tacctttttc tgcgatatgg aacagctgga tttgtttttt
      661 ctaacgttct taaagataaa ctgatttttt tggaatattc atcaatgtcg atgatctgaa
      721 ctgttactgg ctgtccgact tttaacaatg aatggatatt tttcgtataa ccggattgta
      781 cttctgaaac atgaataagt ccttgcgtct cgttatcaag agaaacgaat gcgccataag
      841 gctgtatccc tgtcactttt ccttcaatta tttgtccaat tttataagtc atctacttcc
      901 ctctcttcgc atttatcatt atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac
      961 tggaggtctt aagatgatcg aatttgataa acagatttca agaaaagata cagaaagtgt
     1021 aaaatgggac gcaattgctg agacatatca aatggatgac ttacttcctt tgtgggtagc
     1081 agatatggac ttcttgtctc ccggtggggt agctaaagcg tttagtgaat acatcaaaca
     1141 tggaatcttt ggttatgcca ctctgtctga taaactatat caggctatca ttcattggga
     1201 acgccaacac catgctgtgg agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt
     1261 aaccagttta gctgcagcag tacaaacttt tacaaaacca ggcgattcta ttttgattca
     1321 tgacccagtt tatcctccat tttcctctat tatagaaaca aatcaacgtc acatcgttcg
     1381 aagctctttg ctagaaaaaa ataatcattt catcatggac ttgtctgaca tggagaaaaa
     1441 aatcgaagaa aacaatgtaa aagcaatgat tttatgcaat cctcacaatc ctggagggcg
     1501 tgtttggagc aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat
     1561 tctgtttagt gacgagatcc atcaagattt aactttattc gatcatacat ttacttccat
     1621 gctaacgatt gatcctaatt tagatgatct actagttgct tttacttccg caaccaagac
     1681 tttcaatcta gctgcaatca aaaattcaat ggtatttatc aaaaatccag agatgaaagc
     1741 tgattttgaa aaacatttag tgatgaacca acagcacgaa atcaacactt ttggactgat
     1801 tggaacccaa gcggcttatg agacgggtgg agaatggtta agccaactga ttccttatct
     1861 agaaaaaaat gttcaaacag ttaatcagtt ttttgaacag tatcttccga aagtgagaat
     1921 aatgaaccct gaaggaactt acttaatgtg gttagatttt accgcctata tttcagacga
     1981 ccaagaattg gagaacactc ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac
     2041 gtttggtcct tccggtcatg ggcatatgcg cttgaatatt gcttgtccag aagaaacttt
     2101 actggaagga cttaatcgaa tcaagtatgc tttagagaca atcgattaaa tcatttattc
     2161 aaaataaaag gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata
     2221 ccaaactatt ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt
     2281 ggaccacgtt gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca
     2341 aagactgtat gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt
     2401 tctgatggga atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta
     2461 tttacaataa agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct
     2521 ccgcgaaggt tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt
     2581 tcgccaccca ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg
     2641 cggtgaaaaa tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc
     2701 gtcttcggtg ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata
     2761 acagcttttg gtcctttttc actttttaaa tctagttgtg ggaatgccat attgtttcct
     2821 cctaatattt gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac
     2881 tagatttgtt ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt
     2941 tcgaattcaa acatttttga gaagcattct caattaaatt ctgatatagt gtgaaaaggg
     3001 aggaattttt atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt
     3061 tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca
     3121 acttggtggt caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta
     3181 tccagcaata aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa
     3241 ccatacattc catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga
     3301 gattactaca aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg
     3361 ttcattccag ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt
     3421 ggattatttc gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg
     3481 tggtgattct gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt
     3541 gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc
     3601 atctgtcaac ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact
     3661 gacagatatc cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc
     3721 tctgatcgtc aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga
     3781 tagtaccaga aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta
     3841 tgcagctggt gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg
     3901 tgaagcacct actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca
     3961 gcctggtcac agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc
     4021 tctttgcatt tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa
     4081 catgaaatag agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac
     4141 tttagaagaa aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc
     4201 acaacttgtc atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac
     4261 tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat
     4321 ccaattagat atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga
     4381 agatgacgaa ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta
     4441 cggtacgatc ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa
     4501 agaactaaac agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc
     4561 gatcgccgct gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct
     4621 tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt
     4681 tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa
     4741 tacaaaaaat aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg
     4801 atcagtaacg ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat
     4861 aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc
     4921 gatttgaaac ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa
     4981 caaaatactc gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt
     5041 cacttcataa aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat
     5101 ggttttgaag ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc
     5161 ttgatctaaa gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg
     5221 tcgaaagttg atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa
     5281 tccagcatat tccatccact gccatctcga atttttcata ttagaagctc cattcatcca
     5341 gtgattgttt cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa
     5401 aacctgataa aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt
     5461 catagttatc tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt
     5521 tatccataat gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct
     5581 ctacaaaaga gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct
     5641 tatctgggtt cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta
     5701 ctttttcata ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt
     5761 cccatgtaaa tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga
     5821 cttttttccc ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga
     5881 ctgtctctgg agctacatga atgtcaaact catctgctag acgattagct actgtttctg
     5941 gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt
     6001 ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat
     6061 caattaaata tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat
     6121 gctgtttttg ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt
     6181 tttgctgact ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt
     6241 ccttttttct catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt
     6301 gtcttttctt ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa
     6361 tattcgtaaa gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta
     6421 tccgcttgga aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa
     6481 tcatatcggg ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca
     6541 cgataatcgg acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac
     6601 agtgtcacta gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa
     6661 gcagctgtca gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca
     6721 atttctgttt tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca
     6781 cctatctttt ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct
     6841 cctaccacac ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc
     6901 gttggaaaaa atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc
     6961 agatcgattg gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca
     7021 ttatagacca tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta
     7081 cgtaaaaagt tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc
     7141 aatgtgacat ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg
     7201 acccgccctt ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc
     7261 tttcctcgtt ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata
     7321 tctagactca gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca
     7381 cgttctagat aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta
     7441 gggatggctt tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact
     7501 tcaccgacat actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga
     7561 ccttctctga ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct
     7621 tttgcaattt ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact
     7681 tgtgaacgta aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag
     7741 ccattcggat tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc
     7801 tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt
     7861 ttatcgaata agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac
     7921 tcttctttgg aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact
     7981 tcattcatca gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga
     8041 tccacaaaat cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct
     8101 cgtttacgtt gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga
     8161 tcattcgtat gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat
     8221 cataccatag gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc
     8281 ttgaagcaaa ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac
     8341 attcattttt acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc
     8401 attataatca gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc
     8461 ttcacaagcc caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt
     8521 cgttgaccag tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt
     8581 caaaatcatc ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa
     8641 ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc
     8701 gccgaaataa acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg
     8761 ctctaaggta acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc
     8821 ttccttgatt gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt
     8881 atatcctctt ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg
     8941 aagcatttct gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac
     9001 tgcatcaaga tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact
     9061 ttagcatatg ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga
     9121 ggtgaacaag atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa
     9181 ctctccagca tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat
     9241 caaacaagat tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat
     9301 caacgatcaa aaaaaacacc catggtatca ctatcttttt aaagatgctt cttgtgttca
     9361 agaaaaaagc tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa
     9421 agaatggcaa gatactattt tggaattatt tcgctattct gctgatttct ttttttatac
     9481 agacacagat gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat
     9541 caacggtatt tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg
     9601 ttctttccat tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt
     9661 tttaacagag caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc
     9721 tttacattac tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga
     9781 actgatcaaa caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa
     9841 tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga
     9901 aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta
     9961 tcttttattg cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt
    10021 gtcacaaccc cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt
    10081 ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc
    10141 ataaacagag attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc
    10201 caagtcacta tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt
    10261 gtcatagacg aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac
    10321 ttttcccgat acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat
    10381 tgttactttt gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc
    10441 atagacaaaa aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa
    10501 gttctttact gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag
    10561 caaaacccat ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca
    10621 ttccttttga gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa
    10681 tgcatagcta ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac
    10741 gacttttaaa ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa
    10801 cactaaaagt cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt
    10861 ttcctgctct tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat
    10921 ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg
    10981 aggccatata cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga
    11041 actaacggat tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca
    11101 gctaatgatc tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc
    11161 tttatttttt agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta
    11221 tagcggagat tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc
    11281 tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag
    11341 ttctgcgggt gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc
    11401 tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt
    11461 catttgagca gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt
    11521 ttcaccgggt tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata
    11581 ttcataccca gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt
    11641 gctgtaggga gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc
    11701 cggttttagt ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa
    11761 aaaatcggat gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa
    11821 cgttgtcgct tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga
    11881 aaaataaagg tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag
    11941 atattttcca agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca
    12001 agaaaatctt gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat
    12061 tcaagcgact tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc
    12121 ttaaatagcg aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg
    12181 atagaaaaaa aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca
    12241 gctaagtagt tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg
    12301 attgatttga aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct
    12361 cttgcagtaa ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa
    12421 tattctttac tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta
    12481 cccataaata aataacataa ataattctaa a
//
LOCUS       DAZUMI010000092        12161 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000092 DAZUMI010000000
VERSION     DAZUMI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.093"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..731
                     /locus_tag="ACG81C_002126"
     CDS             159..731
                     /locus_tag="ACG81C_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5065271.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            876..1580
                     /locus_tag="ACG81C_002127"
     CDS             876..1580
                     /locus_tag="ACG81C_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5065272.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1808..2146
                     /locus_tag="ACG81C_002128"
     CDS             1808..2146
                     /locus_tag="ACG81C_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065273.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2131..2985
                     /gene="lgt"
                     /locus_tag="ACG81C_002129"
     CDS             2131..2985
                     /gene="lgt"
                     /locus_tag="ACG81C_002129"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5065274.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3132..3365
                     /locus_tag="ACG81C_002130"
     CDS             3132..3365
                     /locus_tag="ACG81C_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5065275.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3563..5467
                     /locus_tag="ACG81C_002131"
     CDS             3563..5467
                     /locus_tag="ACG81C_002131"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5065276.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5777..6139
                     /locus_tag="ACG81C_002132"
     CDS             5777..6139
                     /locus_tag="ACG81C_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS5065277.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6156..6767
                     /locus_tag="ACG81C_002133"
     CDS             6156..6767
                     /locus_tag="ACG81C_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFS5065278.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6869..7777)
                     /locus_tag="ACG81C_002134"
     CDS             complement(6869..7777)
                     /locus_tag="ACG81C_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5065279.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7819..8133)
                     /locus_tag="ACG81C_002135"
     CDS             complement(7819..8133)
                     /locus_tag="ACG81C_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5065280.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8432..10363
                     /locus_tag="ACG81C_002136"
     CDS             8432..10363
                     /locus_tag="ACG81C_002136"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5065281.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10656..11207
                     /locus_tag="ACG81C_002137"
     CDS             10656..11207
                     /locus_tag="ACG81C_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5065282.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11520..12062
                     /locus_tag="ACG81C_002138"
     CDS             11520..12062
                     /locus_tag="ACG81C_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS5065283.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3951 a         2034 c         2556 g         3620 t
ORIGIN      
        1 gaatttaata gagaagcctg gcgtctatta agaaataatt ttcttgatga gcgtcaattt
       61 ttttaacgga aatctatcct atactaaatt cagtaaagaa aaaggaggaa tcaaagaaag
      121 ctattttaaa caaaccaaaa ttataggagg aataaatcat gactgaaaat aaagctactc
      181 aagaaagaac accccaagaa agattgcaag aagaacaaga acacaaggaa catgttcatc
      241 atacgaaaat taatgcagcg gccattgcgg atcatctttt aggcaacatg catacattac
      301 atgtgaaact gcaccaatat cactggtatg taaaaggagc caatttcttt acgttgcatg
      361 aaaaatttga agagttgtat aacgaaaatg aaaaatggtt cgacaaactt gcggagcgtc
      421 taattacttc tgggcataaa cctgcttcga caacggttga atttgaaaaa tattcgatgc
      481 tttcggaaga tgcagttaat aaatacgcta aagcagaaga catggttgaa aatattatcg
      541 aggacttcag aagcactcgt gacctaacta ttcgcgcgat tcgtttagct caagacgagg
      601 gtgacgatgc tttagaagat acattgattg cttttaaaaa tgacttagac aagaacattt
      661 ggatgctgca agcgttcatt ggtaaagaag cattagaaga tgatgacgct cttgatgagg
      721 atgaagatta aggtaaaagt agaagctgat ttagggattg gaattctttc cagtcctttt
      781 tccattttct tgatgtgcat caattttttt gtagtaagag acttatataa taaaagcagc
      841 gaatataaac aaaataaata aagggggaga atgaattgac taaacatacc catcatgaaa
      901 ttggcgacca cgcagcctgt atccgcttgg ttccaatatt caatcactta gaggatagcg
      961 cgatgaatta cattgctgaa aaagcccaca cgaaacagta tcaaaaagga gagtttctgt
     1021 ttcgttcaca agaaaaagag gatactttat atattatcaa tcgtgggaaa atccgtattt
     1081 atcgattggc agactctggc aaagagcagc tagtgcggat cttgaaccct ggtgatttca
     1141 ccggagaatg gaccttgttc aacccggatg cagtacacga ggaatatgcc gaagcaaccc
     1201 gagataccgt tgtctgtatg attcagcaaa aagatgtcca agattttctg gaacaatacc
     1261 cagctatttc tttaaaacta ttggcggaaa tgtctaaacg gttagaaagt tcagaacgtc
     1321 aaacgacaca agtagccgtg gagagtgtca ttacccgttt agttttgttt ttggcagaaa
     1381 atgtggaacc tgaaatgggc aactctccca ccatcaccct gccgatggca aaaaaggaca
     1441 ttgcttccta tttaggaacg acacctgaga ccatcagtcg caaatttgga gaattggagg
     1501 acgaaggatt gattcaacag ctgtctggga aaaggatcaa gattcaggat ttagatgatt
     1561 tattgcttta cagcgaataa tcggacacac ttttttctac acgttggtgt tctgtcgctc
     1621 ttcattaaca acgggacaga actcagtgct tttgcttcca tctgtccgtt tttggctttt
     1681 ttaagacaga acgaccttca ttcattggaa aaataaaagc acaaacatga taaccctcaa
     1741 gtttttttgt tttatttttc tttaagatag ggttgtcgaa caaaacgtaa aaataagtag
     1801 ggtgaatgtg cattgttttt gggtgatagt tgccatcacg ttgtcagcag cgggggcatt
     1861 ctttgtcgtc cctttcacca agaacacgat tggcggcgaa gcaaaaacgt ctaacgttgt
     1921 tgaggcagat aggttagcta tggaaaaaga agaggaagtt gtaaaaggag ccggcgtaat
     1981 cggacacgga aaagtggcgt attacgaagc gagtgaccag ggagataaaa gtgtattcac
     2041 tgtttggttt ccatggagct tagccggaat cttgttcagc atcaccatga ctagaatgac
     2101 gcgaaaaatt gaagaaatgg agcaaataga atgggattcg tattaggcgt tttgaatccc
     2161 catgtggtgg agattggttc tgttgtgatt cattggtatg gagtcattat tgcagcgggc
     2221 atattagcgg ctctccagtt gagcaccaag gaagcaaaga aaaaaggact ggaagaggat
     2281 accattatcg atatggcctt atgggcaatt ccgattggac tcctcggcgc tcggctttat
     2341 tatgtgttat ttgaacttgg ttattatctg caaaatccag gacaaatcct tgcgatttgg
     2401 aatggcggca ttgcgattta tgggggattg attgcaggag ggggtacgct atattggtat
     2461 gcaaagaaaa aaggtacttc tttggcactc gttcttgata ttttagcgcc caatgtattg
     2521 ttagcccagt ccattggtcg ttggggcaat tttatgaatc aggaagctca tggtgggccg
     2581 gttactcgtc aattcttgga aaatctttat ttacctgaat ttatcattga acaaatgaat
     2641 atgaatggta cctactacca tccgactttt ttatacgagt cattgtggag tttgttgggc
     2701 tttgtcctca tcgtcactat acggaaccga aaacagcttt tgcgtcaagg agaagtcgct
     2761 ttgagttacg ttttgtggta ctcagtgggt cggttcttca ttgaaggtat gcgaacggac
     2821 agtttatgga ttggcgagtg gatccgcgtt tcgcaagcct tgtctctggc cctgtttatt
     2881 ggcgcgattg tagtatggtt tatacgcaga caagattatc caccaatttc ttattattct
     2941 gatggcaata aggggcagaa aaagactatt aagatggtga attgagcaaa aagataaaaa
     3001 gaagtgcaaa acttgatatg cgtcaatttt cgtttgctaa aagtgagcta tactacagtt
     3061 gtaaagagga aaaggcagtg ttcattaatc tgctgccgat aaaataaaaa cacataaagg
     3121 agataggaat tatggaaaaa gctatattgc aattagaaac gttgtcttgt ccaagttgta
     3181 tgcaaaaaat tgaaggtgcc gtgaaatcag ttaacggtat tgataaagac agcattaaag
     3241 tattattcaa ctccagcaaa gtcaaaacca attttgattc agctgtcact tcgattgaag
     3301 agattcaaaa agctatcgaa aacgtaggct atccggttct taaagcaaaa gtcaaggccg
     3361 tttaacagat taaatgaact aggcaattct ggaagaaaag ctatttttcc aggattgctt
     3421 tttttgttgc aatccgattc gaggtgctta gaaacgataa aagagaactt tctaaacttg
     3481 atgtcagtca attaattact taggcaatta tcttatactg tgtttatcaa taaaagagaa
     3541 attaaatttg gagggaaaaa gcatgcaaca atatatatta agcaagaaaa atgttatcac
     3601 cgtcatcagt ggattgttaa tcgtactcgg tttcttcagt cactttgttt tagaaaacgt
     3661 aggactttca gagtggtctt tgatcatcgc ctctgtcttt gggattacgc caattgccat
     3721 tcaagcgttt caagcaatga aagtcaaagt catcagtatt gatgtcttag tcagtattgc
     3781 agcgattggt gcgcttttta ttcaaaatta tgaagaatcg gctattgtca cgttcttatt
     3841 cttattcgga cactacttgg aacaacgaac attgaaccaa acgcgatctg ctatcaaaga
     3901 attgactgaa atggcacccg aaagcgcctt gaaacaaatg gataatggcg aatttgaaga
     3961 agtagaagtg gatgatatag atgaagggga tatcttgctc gttaaaacgg gtgcgaaagt
     4021 tccagtagat ggcatagtcc ttacgggtga agggcatatc aatgaagcta gcattacagg
     4081 tgaagctatt ccggtcaaca agctagctga tgcagaagtt tttgcaggaa ccattttaga
     4141 aaacggaacg attcaaatca gagctgaccg agttggtgag gacaccacat ttggaaaaat
     4201 tattgaactc gtggaagaag cacaagattc taaatccgaa gcggaacggt tcattgatcg
     4261 cttttctaaa tactacacac cagctgtctt ggttctggca attgttgtat gggcgttcag
     4321 tcaaaatatt gagttagcaa tcaccatctt ggttctaggt tgcccaggcg cattagttat
     4381 cggagtgcct gtctcaaacg ttgccggtat tgggaatggt gctcgtaacg gtgttctttt
     4441 aaaagggagt gaagtcattc aagacttcag taatgtggat acaattgtat ttgataagac
     4501 aggtacttta actgtcggaa acccaaccgt tgcagcgact gaactgtatg aaaaagatcc
     4561 tgctgaaacg cttggctatc tggccagtgt ggaacgggaa tcagatcatc cattagcaaa
     4621 agcggtctta aatcaaattg gagaaacaaa cttttatcct gttgaaggaa ctgaagttgt
     4681 gaaaggcggc ggaatcgttt caagtgtagc tggacataga gtggctgttg ggaatgtggc
     4741 cttgatggaa aaagaaaatg tcactctcag caaaaaagtg caaaaagatg tcaaacggtt
     4801 tgaacaacaa gggaactctc tcgttttgac agcggtcgac ggtgaattaa aagtactgat
     4861 gggcattcgc gatcaagtcc gtccgggtgt gaaagctaat ttgcaggaat taaaagactt
     4921 gggcgtgaaa aatctagtcg ttctttcagg agataaccaa ggaaccgttg atgtggtcgc
     4981 cggcgaactt ggtttgaccg aagctcacgg ctacatgttg ccggaagaca aatcggctta
     5041 tatcggaaaa cttcaacaac gtggtcaaat tgtagccttt gttggagatg gcgttaacga
     5101 tagtccgtcc ttagctttag cagacattgg aatcgcaatg ggaagcggaa cggacgtagc
     5161 gattgaaaca tccgatgtcg ttttaatgaa ctcgaacttc agcaacttgc ctcacgcatt
     5221 aggattagta aaagccaccg caaataatat gaaacaaaat atcgtgattt caattggagt
     5281 agtgttggtc ttgttgacca gcgtcttctt cagcgaatgg atgaatatgt ctatcggaat
     5341 gttggttcat gaaggtagta tcttggtggt aattttcaat ggcatgagat taatgaagta
     5401 taagttgaaa ggtcgaaaag aacaaaaaga agtatcagca cctgcagaga aagtaaaaat
     5461 atcttaagaa taaattagaa gaggctgaga caatagtcca tccttgaaat gacaaaagcg
     5521 gaaactcaga tttttaaatc tgggattttc cgcttttttg agttcttgaa aaaaaataag
     5581 agtatagagt atgttcttta taaaattaat tttcgtgtaa atgattaaaa aatttaataa
     5641 taaaatttaa attcctatta agttgtaaca catgaaattg cttataatag tgtattgaaa
     5701 ttaaggtaga ttgacaaacg agtgatcaaa tagatatact accttcaaac gaccgtttga
     5761 atgtgaggtg agatgaatgg aaaaagtttg ccaagtgact atcgtacata aagaaaaagt
     5821 aaatgtaata aaaaaaaaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     5881 atgtttcagc gattcttcta ggattaaaat tttctatgct ttagaaacct ataaggaaat
     5941 gtgtgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     6001 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     6061 ttctttagcg aatgaagaca tcctttccgc tattcgtgtt tttttaaagt tatcagaaaa
     6121 tctatctatg aaatcttagt ttggaggaag ttattttgtt acaaagtatt ttttcagcta
     6181 tcgctgttta tatttctacc agtattgatt atttgtttat cttgctgatt atcttttctc
     6241 aaagccacac aaaaaaaggt attcgtcaca ttttttgggg tcaatatctt ggaacaggta
     6301 ttttggtagc cgtaagtttg tttgctgctt atgtgctaaa ttttattccg caagattgga
     6361 tcattggtct tctcggtcta attccgattt tcttaggaat cagagtcgct tttgttggcg
     6421 aagaggagga agaagaagag gaagtcgttg aaaaactcga atctagagga actaatcgct
     6481 tattctggac agttgcctta ataacgattg cttctggagg cgataattta ggtatttata
     6541 ttccatactt tacctcgtta gctttttcag aaatcattat tgctttaatc gtattcgcta
     6601 tctctgttgc ggttctctgc tatatcagtt acaaattagc caagatttct tttgtttccg
     6661 aaacgttaga aaaatacgaa cgaatcattg tccctataat gtttattgcg ttaggaattt
     6721 ttattatgat agagaacggg acgatacaaa cgctactagg tttttaaaaa tcataatcac
     6781 gaaagataaa aagagaggca gcgactaatc aaaatcgctg ccttttttgc ttttcaaata
     6841 atgaagtatt ttttagcgta gaactatctt acgtgttgtg ggagtatccc ttttcaagct
     6901 cttctttgct tgtagttatt tctattgtga tatgattctc tacgcttgat ttgtgtgcga
     6961 cttctgcaat ttcttctttt atttccctag ccttgtctat gtctgaaggg tctataagaa
     7021 ccgtgatgat aagggcgttt tcttccccgt ccagtgacca aatgtgaaaa tgtgagaggt
     7081 cttttacagc aggaacattt ttaatggagt ccgccaagct ctttacgttt acattttcag
     7141 gcgaaccatt caataaaatt ttcattgtgg tgaaaaattc aggaacggtt ttgtagagga
     7201 tataaagtgc aatcaaaatc gataagatag gatccagacg atatgcctcg gtaaagttca
     7261 ttagaatact cacaatcaag actccaatcc accctaatac gtcttccagc atatggaggt
     7321 tcaacatgct ctcgtttttg gatgtgcctt tgcttaaaat ccacgcagca tatccattca
     7381 atgcaatagc tacgagagaa agccagaaca tgccactata atttacgggt tgtgggtcaa
     7441 tcaaacgtgg cacactacga tagatcataa aaaacgagcc actaattagc acaacgccag
     7501 ttatcagcgc gcccagtaaa gaaaagcggt tatagccaaa agtgaaccgt tcatcttgtt
     7561 ttttctctga ataaccttga aaaaaccaag cgaatccaat tgaaatagag tcccctaaat
     7621 catgaactgc gtcggacata atggacacac tattgaaaag aaagccaaag aaaaattcgc
     7681 ttatagagaa aataagattt atgaagaaag caattttaat attctttttc ggttgattct
     7741 gattcgagtg attatggtct ttatctgatt ttgacattta aaaacctcct aagtttgtat
     7801 gctacatact aattggtttt atttttctaa ttcattaaca tgagttaaga cttgttcgag
     7861 aatactaaaa acatgaaggt catctaaact gtaaagcata ctttttcctt ctcttcttga
     7921 tttcactaag cgtgcgtctt tcaacgtttt taattgatga gaaatagcgg actgttccat
     7981 gtccagagaa atagcgatat ttccaacact acactctttt ttctgtaaaa gaaagagaac
     8041 cgacaatcgt gtaggatcac taatcacctt aaatagtttg cttattgaca gaatagatcc
     8101 tttatcgatt ggagaagttg tttttatatc catagtgttt ttattccttt catatgacct
     8161 tccgttcata tctatattac catatgagcg aaacaatgct aatcttttgt ttataaaaga
     8221 gaagtggcgt tctctttatc ttgacaaaat gtatatagcg atataaaatg agtatacaaa
     8281 catatgatta attagtcata tgtaaggtgg attcattacg atttactttt ggaataaacg
     8341 aaaggagtca gaaatagcag aaatttatgt gattcaagaa gtaaatgatg gaaaacaaag
     8401 gaattataaa ttatataggg aggcaataga agtggaaact atccaagaac aacagtctca
     8461 agaaaagcat aatcataatc acaaccacga tcacgaccat gatcatggga aaatgccagt
     8521 cgttttgtac tttgttggtt tagcattagc agtgattgct ctatttttga atgaagatta
     8581 tcagttggta caaaatgttt tgttctcaat cgctacaatc agtgccgggt atcacgttat
     8641 tcttcttgaa ggccttggag aaacaattga gaattcaaaa gcacaaaaaa agttcatacc
     8701 taattctcat attttaatgg gattagcggc aattggagct tctttgatgg ggaatttttg
     8761 ggaaggaaca ttattgattc ttattttttc tggcgcacat tttttggaag attatgcaga
     8821 aggaagaagc aaacgagaaa taacgaaatt actcgaaatg aatccaacga ctgcccggtt
     8881 aatcctgcct gatggtagta caaaaaatgt ggatgtcagt gaattaaaag tgggagatca
     8941 acttcaagtg ttgaatggcg atcaagtacc tattgatggc gtcattttgt ctggatctac
     9001 atccattgat gagtcgtcta ttactggaga aagtatcccg aaagagaaat ccaaaggaga
     9061 tggcgtcttc gggagtacaa tcaatggaac aggtaccttc acgatgaaag tcaccaaaga
     9121 aaacaaagat acagtatttt caaaaatttt acaattagtg aatcagaacc aagataacca
     9181 aacaaaagct gcgagtatta tccaaaaatt tgaaccgaaa tatgtcacag ttgttttaat
     9241 tgccattcct ctattcatgt tattggctcc ttttctactt gattggacat ggtcacaaag
     9301 tgtctataga ggattggtcc ttttagttgc agcttcaccg tgtgctttag cagcagctac
     9361 tgtctcggca acgctatcga cgacttctaa tttagccaaa aaaggagtcc tttcaaaagg
     9421 tagttcttac ctgtctcaat tagccgatat taaagcaatt gcatttgata agacgggcac
     9481 cttgaccaaa ggaaaacctg aagtaaccaa ttattatttt gctgattccg tgaacgaaga
     9541 aaacatgatt gatatcgtag tagctcttga aaaagaatcc aatcacccct tagcagatgc
     9601 tattctaaga aagtttgagc aaaaaaacaa actaactatt gaagtagaaa accaaattgg
     9661 taaaggattg acgggagatt acaaagggag aaattatcgt ataggaaaac cgacttcgtt
     9721 tgatgatgtt tcagatgaat atagtcgttt aaataatgaa tgggcgtcag aaggtaagac
     9781 agtcgtatac gtagcagtag atgaagaggt tataggcctt attgccctta tggacgttcc
     9841 gagtgaatat gctaaagaaa ccattgaata ttttaggaaa caaggtatac acaccacatt
     9901 aattactggt gactcagaaa tgacgggaaa agcggttgct aaacaattag gaatagatga
     9961 agtgattgcg aatgtcatgc cggaagacaa atccagaatt atagacgaac aaaaagaaaa
    10021 atacggcgtg accgccatgg ttggagacgg tgtaaatgat gctcctgctc ttgttaatgc
    10081 agatgtggga atcgctatgg gagatgggac tgatgtggca gtagaggtat ctgatttagt
    10141 tttaatgcaa aacaatttat ctaaattggt acaatctcat aatatttctt cgaaaatgaa
    10201 ccgtgttatt tggcagaata ttattttttc aatggccgtt gttgcttttt taattgtcgt
    10261 tagtttctta ggcttaacgg atattgcaat cagtgtcatt attcatgaag gaagtacttt
    10321 ggttgttatt ttgaatggac ttcgattgct aaaggcagtc taaagaatga ttgcacaact
    10381 tcaaattaat cgagtaatag agataattaa ggattacact acatttttta tctctattac
    10441 ttgtttatat ttatctagat gttcaaagtc aaaatgatat atcaaccata ctcttgtggc
    10501 gcgggttgaa tataagatac atgaaattgg ataaaatcat gtatcaagaa tcctatcctt
    10561 atagggttct ttttttttac tccccctaaa cacacgattt cgtgttgtaa taactataat
    10621 catgtattcg aacagactaa aaaggaggga ttgtcatggg ctataatgtt cagccactac
    10681 ggtcgcaaca ggagattaat gattttttat tttgcctgcg acggaataaa aatgcggaac
    10741 gtgacgtttt cttgtttcta attggaatta acagcggtct gcgcatgtcc gacatcgtta
    10801 aactcaagaa aaaagacgtg attacctcaa aaaacccgcg catcgtggag caaaagacag
    10861 ggaaaacacg cattttatac ttaagcagct tacaggactt gattcacggc tataccgcac
    10921 ccttagatcc ggaggcttac ttgtttccca gtactaaggg cggccattta gaagtcaata
    10981 cggtctacca gatgtttcaa aaggtcgcga agctgttagg aagggacgat atcggcaccc
    11041 acaccctacg aaaaaccttt ggttaccact attacaagaa aaccaaagac gtggccacct
    11101 taatggaaat ctttggtcac agcagcgaga agattaccaa acgctacatc ggaattaatg
    11161 aagatgaaat cagtgaaacg ttattaaatt ttcggttagg tttttgattg tattatataa
    11221 tagataagca cagtaagacg gttataatca cccacagtat tggacaaatc aacctttttt
    11281 tggtatatta tatatacaaa tgagagcagg ctcatagtca acttgatata aatccggaac
    11341 ataagggtcc ggcgggtggc gagcaccaaa actaaactcg atacaaatcc ggaacgtaag
    11401 ggtccggcgg gtggcaagca ccaaaactaa acctgtacaa aaggctgctc ttttgagcag
    11461 ctttttttat tgggaaaaag gagcattctt cgcacgcggc aaaaaaagaa tacaaacgca
    11521 tgtctataaa agagacaacc caaattacca gacagttaaa cgcgatttat aaggctgctc
    11581 atctcttaca agaacatttt gtagataaaa aagtatcctt tgtcggagaa gtctcaacgg
    11641 tagcgattat tttttcgaca actaatttta tgcacctctg tggcattgat tatcgcagag
    11701 gcacacactt gttttttcaa gatgccttag acaggaaaat aaatttacag gatattcaaa
    11761 tcaaaacgga tgggacgacc tttcagaaat tacaagtaat aggtagcctt gatttattat
    11821 tggggaagca tatttctatt gttggcagag gtgtttactc ttctcttcgc tatgatgcag
    11881 ctattcgaac aaggaaaaaa atattggctc tgtctttgaa acaaaacgga ttaatctata
    11941 tccctatctc tttgttaaac ctatcatcaa aggaaattgg acctggacaa aaagttactg
    12001 gcatttttag cgaggattta acatccgggg aacttaaaat gattatggaa gtcatcgatt
    12061 aatcattaat agtgatatgc gacgtcattt tgaagtaatt attctatttt gggttataaa
    12121 ataaagcatt ttaagacgat ttcaaatcat tcatgtgggt t
//
LOCUS       DAZUMI010000093        12144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000093 DAZUMI010000000
VERSION     DAZUMI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.094"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG81C_002139"
     CDS             <1..566
                     /locus_tag="ACG81C_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5065284.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACG81C_002140"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACG81C_002140"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS5065285.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACG81C_002141"
     CDS             1588..2160
                     /locus_tag="ACG81C_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065286.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACG81C_002142"
     CDS             complement(2412..2669)
                     /locus_tag="ACG81C_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065287.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACG81C_002143"
     CDS             complement(2795..3610)
                     /locus_tag="ACG81C_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065288.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACG81C_002144"
     CDS             4127..5452
                     /locus_tag="ACG81C_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5065289.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACG81C_002145"
     CDS             5445..5795
                     /locus_tag="ACG81C_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065290.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="ACG81C_002146"
     CDS             5792..5962
                     /locus_tag="ACG81C_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065291.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG81C_002147"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG81C_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS5065292.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACG81C_002148"
     CDS             complement(7113..8066)
                     /locus_tag="ACG81C_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5065293.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACG81C_002149"
     CDS             8678..10171
                     /locus_tag="ACG81C_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS5065294.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACG81C_002150"
     CDS             10306..10602
                     /locus_tag="ACG81C_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5065295.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACG81C_002151"
     CDS             10703..11554
                     /locus_tag="ACG81C_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065296.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACG81C_002152"
     CDS             11575..11787
                     /locus_tag="ACG81C_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065297.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4172 a         1863 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DAZUMI010000094        12105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000094 DAZUMI010000000
VERSION     DAZUMI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.095"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..554)
                     /gene="frr"
                     /locus_tag="ACG81C_002153"
     CDS             complement(<1..554)
                     /gene="frr"
                     /locus_tag="ACG81C_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5065298.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(557..1279)
                     /gene="pyrH"
                     /locus_tag="ACG81C_002154"
     CDS             complement(557..1279)
                     /gene="pyrH"
                     /locus_tag="ACG81C_002154"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5065299.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1415..2296)
                     /gene="tsf"
                     /locus_tag="ACG81C_002155"
     CDS             complement(1415..2296)
                     /gene="tsf"
                     /locus_tag="ACG81C_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5065300.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2395..3177)
                     /gene="rpsB"
                     /locus_tag="ACG81C_002156"
     CDS             complement(2395..3177)
                     /gene="rpsB"
                     /locus_tag="ACG81C_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5065301.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3536..4015
                     /locus_tag="ACG81C_002157"
     CDS             3536..4015
                     /locus_tag="ACG81C_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5065302.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4232..5323
                     /gene="phnW"
                     /locus_tag="ACG81C_002158"
     CDS             4232..5323
                     /gene="phnW"
                     /locus_tag="ACG81C_002158"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5065303.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5316..5444
                     /locus_tag="ACG81C_002159"
     CDS             5316..5444
                     /locus_tag="ACG81C_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5065304.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5448..5993
                     /locus_tag="ACG81C_002160"
     CDS             5448..5993
                     /locus_tag="ACG81C_002160"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS5065305.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6447..8138)
                     /gene="argS"
                     /locus_tag="ACG81C_002161"
     CDS             complement(6447..8138)
                     /gene="argS"
                     /locus_tag="ACG81C_002161"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5065306.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8558..9505)
                     /gene="arcC"
                     /locus_tag="ACG81C_002162"
     CDS             complement(8558..9505)
                     /gene="arcC"
                     /locus_tag="ACG81C_002162"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5065307.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9620..10639)
                     /gene="argF"
                     /locus_tag="ACG81C_002163"
     CDS             complement(9620..10639)
                     /gene="argF"
                     /locus_tag="ACG81C_002163"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5065308.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10730..11959)
                     /gene="arcA"
                     /locus_tag="ACG81C_002164"
     CDS             complement(10730..11959)
                     /gene="arcA"
                     /locus_tag="ACG81C_002164"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5065309.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3305 a         2518 c         2192 g         4090 t
ORIGIN      
        1 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
       61 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
      121 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
      181 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
      241 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
      301 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
      361 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
      421 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
      481 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
      541 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
      601 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
      661 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
      721 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
      781 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
      841 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
      901 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
      961 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
     1021 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
     1081 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
     1141 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
     1201 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
     1261 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
     1321 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
     1381 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
     1441 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
     1501 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
     1561 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
     1621 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
     1681 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
     1741 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
     1801 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
     1861 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
     1921 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
     1981 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
     2041 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
     2101 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
     2161 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
     2221 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
     2281 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
     2341 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
     2401 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
     2461 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
     2521 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
     2581 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
     2641 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
     2701 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
     2761 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
     2821 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
     2881 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
     2941 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
     3001 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
     3061 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
     3121 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
     3181 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
     3241 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
     3301 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
     3361 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
     3421 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
     3481 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
     3541 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
     3601 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
     3661 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
     3721 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
     3781 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
     3841 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
     3901 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
     3961 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
     4021 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
     4081 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
     4141 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
     4201 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
     4261 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
     4321 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
     4381 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
     4441 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
     4501 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
     4561 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
     4621 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
     4681 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
     4741 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
     4801 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
     4861 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
     4921 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
     4981 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
     5041 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
     5101 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
     5161 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
     5221 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
     5281 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
     5341 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
     5401 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
     5461 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
     5521 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
     5581 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
     5641 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
     5701 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
     5761 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
     5821 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
     5881 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
     5941 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
     6001 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
     6061 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
     6121 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
     6181 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
     6241 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
     6301 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
     6361 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
     6421 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
     6481 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
     6541 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
     6601 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
     6661 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
     6721 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
     6781 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
     6841 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
     6901 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
     6961 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
     7021 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
     7081 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
     7141 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
     7201 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
     7261 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
     7321 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
     7381 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
     7441 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
     7501 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
     7561 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
     7621 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
     7681 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
     7741 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
     7801 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
     7861 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
     7921 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
     7981 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
     8041 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
     8101 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
     8161 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
     8221 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
     8281 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
     8341 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
     8401 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
     8461 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
     8521 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
     8581 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
     8641 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
     8701 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
     8761 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
     8821 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
     8881 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
     8941 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
     9001 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
     9061 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
     9121 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
     9181 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
     9241 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
     9301 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
     9361 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
     9421 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
     9481 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
     9541 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
     9601 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
     9661 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
     9721 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
     9781 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
     9841 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
     9901 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
     9961 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    10021 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    10081 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    10141 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    10201 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    10261 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    10321 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    10381 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    10441 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    10501 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    10561 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    10621 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    10681 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    10741 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    10801 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    10861 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    10921 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    10981 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    11041 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    11101 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    11161 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    11221 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    11281 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    11341 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    11401 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    11461 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    11521 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    11581 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    11641 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    11701 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    11761 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    11821 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    11881 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    11941 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    12001 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    12061 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcg
//
LOCUS       DAZUMI010000095        11972 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000095 DAZUMI010000000
VERSION     DAZUMI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.096"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..873
                     /locus_tag="ACG81C_002165"
     CDS             94..873
                     /locus_tag="ACG81C_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065310.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            919..1863
                     /locus_tag="ACG81C_002166"
     CDS             919..1863
                     /locus_tag="ACG81C_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5065311.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1879..2658
                     /locus_tag="ACG81C_002167"
     CDS             1879..2658
                     /locus_tag="ACG81C_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5065312.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2670..3368
                     /locus_tag="ACG81C_002168"
     CDS             2670..3368
                     /locus_tag="ACG81C_002168"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5065313.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3396..4160
                     /locus_tag="ACG81C_002169"
     CDS             3396..4160
                     /locus_tag="ACG81C_002169"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5065314.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            4174..5991
                     /locus_tag="ACG81C_002170"
     CDS             4174..5991
                     /locus_tag="ACG81C_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS5065315.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            6003..6968
                     /locus_tag="ACG81C_002171"
     CDS             6003..6968
                     /locus_tag="ACG81C_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS5065316.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVV
                     LVSSEKVIDICENKILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSSSVNTFIV
                     NNEKELLFFSEYIDNPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKG
                     QIIKDRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGAN
                     SPEYIIKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            6961..7656
                     /locus_tag="ACG81C_002172"
     CDS             6961..7656
                     /locus_tag="ACG81C_002172"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5065317.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            7653..8771
                     /locus_tag="ACG81C_002173"
     CDS             7653..8771
                     /locus_tag="ACG81C_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFS5065318.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            8761..9381
                     /locus_tag="ACG81C_002174"
     CDS             8761..9381
                     /locus_tag="ACG81C_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5065319.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            9577..10677
                     /locus_tag="ACG81C_002175"
     CDS             9577..10677
                     /locus_tag="ACG81C_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5065320.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYG
                     GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQ
                     TLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNL
                     YSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHE
                     GMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYE
                     QRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            10684..11079
                     /locus_tag="ACG81C_002176"
     CDS             10684..11079
                     /locus_tag="ACG81C_002176"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5065321.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            11076..>11972
                     /locus_tag="ACG81C_002177"
     CDS             11076..>11972
                     /locus_tag="ACG81C_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5065322.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAIC"
BASE COUNT         4416 a         1799 c         2371 g         3386 t
ORIGIN      
        1 atttaattat gttttttaaa ataaaaagct gtcattttta tgtatgtgaa ttatatctta
       61 atttatctat catcaatact taggagtgaa tctatggaaa agccattcgt caagtcattg
      121 actgctgtgg cggtaggtgc aggtgctatt gcgatctgtt tgtttggtta ccatatcaat
      181 aatcagcggc aacatcagca acggatcaat tatgcagaat ctgccatcac caatcaaaaa
      241 gacaccttga cttctttatc aaaagaagta gacaagctgt atagtacaaa agaaaaaaat
      301 tttctaaacc ctgaaataac agaagaaaca atcacaagtt tgtcaaataa gctatcctct
      361 gtcaaacttt cagcagatga ttacgacatc aaagaatcag aattgcctaa agaagctgtt
      421 gcaatccaag atgagaagaa agcaatcgtg acacagttag atagtgcaga atcaaaactg
      481 aagatccaga cagctgtcaa cgatttattt actaaaaatg tatcaaactg gcagcaagct
      541 gtagacgatg tcgtcatcaa agaaaaggtg acaagtgcag atgtcgctcg tgtacgtgaa
      601 aatatgagct tttttaaaga tagtccttgg aaaacggttg ttatgcaata tctaggattc
      661 gctgatactc agatcgcgca agtcacgcaa ctagatcagt tattcgatac gatgttgaag
      721 gatggtcaag tgactgccac cgctacttac gatcaatatt tgacggcgct cagtcaaata
      781 gagcagatcc ggaatgaaaa aataagcgca gcatacgcaa caaaagcaca aactgttgct
      841 cagcaaatgg gctatagcaa tataagttat tgacagttaa aacgttgatt caagcaaggt
      901 tagcaaacgg aggaaactat ggcaagacga agaagaaaat caagagggaa aaaaatattt
      961 ctgattattt tagcagttct tatcgtatta gtattagcaa ttgtaggagt aggagcaaaa
     1021 ttatatctgg atctctcaca ttctattgag aaaacttacg aatcagtgga acgaacccaa
     1081 ggagaacgaa cgaaaccagt tgatctggac aaacaagaat ctttctctgt tctattgatg
     1141 ggcattgata ctggagattt aggtcgggtc gatcaaggaa gatcagatac gatgatggtt
     1201 gccacagtca gtcctgaaga caaacagaca acgatcgtca gcattcctcg agatacgtat
     1261 gtcgacatcg taggtcgtga cacaaaagac aagatcaacc acgcttacgc attcggcgga
     1321 gctgccatgg cgatggatac cctacaaaaa tatgtagaca tcccaatcga tcactatgtc
     1381 tctatcaata tgaaagggct aaaggaactc gttgatgcag ttggtggtat cgaagtcaat
     1441 aatgacctca ctttctcaca agatggctat gactttccaa tcggtaaaat tacattgaac
     1501 ggggatcagg cattatctta ttctcgtatg cgttatgaag atccaaatgg tgactatggt
     1561 agacaagagc gtcaaagaaa gatcgtagaa ggtgtagcaa aaaaagtatt gagtttaaac
     1621 ggtgtcagca actatcaatc cgttttgaat gctctagagt cgaatatgaa aacagacatg
     1681 agctttgatg aaatgaaaaa aatcgcttta gattatcggg aggcatttgg tacgatcaag
     1741 caagatcagc tgcaaggaga aggcttcatg caagataata tctcctatca aaaagtcagc
     1801 gatcaagaac ttgatcgtgt gaaaaaagaa ttaaaagatc aaatgaatct agagaataaa
     1861 taggaaagtg gagaaaaaat ggaagagaca atcagcttga aagaaatttt tgaaacatta
     1921 cgcaaacact tggtaacaat catcatctca atgtttgctg gcttggccat ctcaggaatc
     1981 gtaacatttt tcatcttgac accaaaatac agttcacaag cacaattgat cgtgacactg
     2041 ccacaatcag acacagcaaa tgtcaatgat gtcaatacga acctgcaaat gatcaatacg
     2101 tataaagata tgatcgtcag tgatttagta ctaaatgaag tcaaagatcg tttagaaaca
     2161 gaagacaatg tgaaaatgac tgctggacaa atcaaagatg cgatctccgt caatcagtca
     2221 caaaattcac aaatgttctc gatccaggca ataagcacga atgcagtgac tgctcaacag
     2281 atcgctaaca caacttcaca agttttccaa gagaacgcaa aagacgtgat gaatgtcgat
     2341 aaaatctcga ttatttcagg tgcagttgcc aatacgacac ctgtttcgcc aaataataaa
     2401 ttgaatctag tgattggctt agtattagga atgatggtag gcgtaggtct ggcattctta
     2461 ttcgaattac ttgatcggac agtgaaagat gaaaaatatg tgacggatgc attaggattc
     2521 ccaatcttag gaacagttcc agaaatgtca gcgaaagaat tgaacgctgg tatccgtaaa
     2581 aaaggaacag ctcctgcacg accaaaagct acaaacgata aaacagcccc atcacgcaga
     2641 agccgctcaa gagtctagga ggaagaaaaa tggcacgaac acagaaacaa aaagcagcac
     2701 aaactcatgc agtcagtttg atcacattaa caaacccatc ttcaccaatc gccgaacaat
     2761 atcgtacgat ccggacgaat atccagtttg cctccgcagc tggtcagcaa atcaaaacga
     2821 ttgtcgtgac ttcatcaggt cctggagaag gaaaatccac cactgcagca aacattgcag
     2881 tcgtttttgc taaatccggt caacgagttc tactagtcga tgcagattta cgtaaacccg
     2941 tcgtctataa gacattccag ctgaacaatg ccaggggatt aagtacagca cttagttcat
     3001 caggaagtgt agcagatgta atccaacgaa cgccagtcga gaacttgtcg attttgccta
     3061 gtggacctaa gccacccaac ccatcagaat tgttaagttc gccaagaatg gatcagatcc
     3121 tagacgaagc aagacagtta tttgacgtgg tgatattcga tatgccacca gtagtagccg
     3181 taacagatgc acagatcatg tcttcaaaaa cggatggcac tttattggtc gtacgtgaaa
     3241 atacatcacg taaagaatca ttgaataaag caaaagaact gctagagatg gtacaagcac
     3301 gagttttagg tgttgtatac aacggagcag aacatagcaa agatgctggt tattactacg
     3361 gaaattaagg aataagaaga gaggagacaa gagaaatgat cgatctacat tgccatatcc
     3421 taccaggaat cgatgacggg gcagaaaact tagaagcaag tattgcaatg gctgaaaaag
     3481 cgattcagca aggcatcacg catattttat gcacaccaca ccacaataat ggaaaataca
     3541 gcaatgaaaa atcacaagtc atttctctag tcgcttcgtt acaagccgaa ttagagaaaa
     3601 gacagttgcc tttgacgtta ttggaaggac aagaagtacg tattaccgga acgctcatcg
     3661 aagacatcca ccgagatgaa attttattta cagatttaga tgatacatat cttctgatcg
     3721 aatttccgac actggaagtg ccgttatatg cagaacggct tttttttgag ttgttagaac
     3781 taggaaagac accagtgatc gtccatccag aacgcaacgc gcattttcgc gaagacccga
     3841 atcatttgct tccgttccta gatatggggt gtttggccca acttactgcg ccaagttatg
     3901 ttggggtatt tggcaaagat attcagaaaa cagctaaaac aatggtcaaa cataatctcg
     3961 tgcagatgac tgcttctgat gcacacggag tctctaagcg caccttttac ttaaaagagt
     4021 gctatgagca gattgccaag gactttggta aggaaaaagt agtacagatg aagcaagttg
     4081 caaaggatct gatcaacggg gatgaaatca gttatccaga ctatgaagag gtaaaaaaga
     4141 agaagtttgg tttattttag ggggaattaa aaagtgttta tattaacaag aagaagaaaa
     4201 attgccacat tggtttttgt ggacagcttg ttattaattg ctgcaaatat tgcagcagct
     4261 aaattcatga agccgtttgt atctatacca atggatctta ttttaatctc tatcggtttg
     4321 tcgattggtt tttatttatt ttatggtagt ttattcaaag ttttcactcg gatcaatcgc
     4381 tatacaaatt taagagagat cattgcaatt tttagttcac tatctgcaag tgctgcctca
     4441 agtatcctca tactgctgtt cattaatcgg cgatacagtt tacggttagt tatctttgct
     4501 tatctgcttt cgctcttatt gatcatcggt agccgattga tctggcgtat ctacatcgaa
     4561 acaaaaaata tgagatacgt atcagcagat tctgctaaaa atacattgat tgttggtgct
     4621 ggcgaaggcg ggcgtatttt atacaacagc tttcttggat ccaaaacggc tcaagatatc
     4681 cacgttgtcg ggtttgtaga tgatgatcca aataaaagaa atacgtatct ttcaggaaaa
     4741 aaagtattag gtgcattaaa agatattccg gaattgattg aaaaatatga cattcaaatg
     4801 gtaacgatcg ctatcccttc cttatcaaga aaaaaattac gacgaatctt cgagttagtc
     4861 gaatccgctc gtgtgaaagt caacacgatg ccttctatag aagaacttgc ttcagggaaa
     4921 attagcgtat caaaattaaa aacaattgac gtagtggatt tactgggaag agatgaggta
     4981 aagctggata ttgaatccat caaagatcag atcacagaca aagtcattct tgtcaccggc
     5041 gcaggaggat cgatcggttc agaaatctgt cggcaaatca ttcaattcaa tcctgcaaag
     5101 ctattgcttt taggacatgg ggagaactcg atctacttga ttgaccgtga gttacgcacg
     5161 catcatcaaa attgtcctac agaaatcgta ccgatcatcg cagatatcca agatcgcgag
     5221 aaaatcaatg agatcatgga acaatatcat ccagatatcg tctaccatgc agctgctcat
     5281 aaacatgtac cactgatgga atataatcca aaagaagcag tcaaaaacaa tattttcggt
     5341 acaaaaaatg tagcagaagc agctaaagca gcaaaagtga aaaacttcgt catggtatcg
     5401 acagataaag ctaacaatcc tccaaatgtc atgggttcaa caaaacgaat cgctgaaatg
     5461 attgtgactg gattgaatga agaaggctgt acgaaattct cagctgtcag atttgggaat
     5521 gtccttggtt ctcgcggtag tgtgattcca gtattcagag aacaaattgc tcaaggtggt
     5581 ccaatcactg taacagactt tcgtatgact cgatacttta tgacgattcc tgaagctagt
     5641 cgtttagtta ttcaatctgg cgcattagca aaaggtggcg aaatttttat tctagatatg
     5701 agtgaaccag tgaagattgt agacctagcg aaaaacatga tccgattgag cgggtatagc
     5761 gaagacgaaa tcgaaatcat tgaaacaggt atccgtccag gggaaaaact atatgaagaa
     5821 ctattgttgg ataaggaaag aaatgatgaa gcagtttatg agaaaatctt tgtaggaaac
     5881 atcaaaggtt actcaataca aaaagtgatg gattttgtca agagcctgcc gcaagacgat
     5941 gaacaattgg ctaaagacat tgtcactttt gcaaatgctt cgaatagata ggagattaaa
     6001 aattgaatat tttaatatta agttgtggac gtcgttgtga actagtgaat gcatttaaaa
     6061 cagcattaaa caataatgaa atggaaggaa aaatcattgg agcagattgc tctgaactag
     6121 cacccgcact atatatgtgt gacgaaaaaa tagtattacc taaagtttca gaaagtaatt
     6181 atattgagga attaataaaa gtaataaagt tttttgaaat taaattaatt gttcctacta
     6241 ttgatactga attatcaaca ttatcaagaa ataaaaaata tattgaaaat gaaacagact
     6301 cagttgtatt agtttcttca gaaaaagtca tcgacatttg tgagaacaaa attttaactt
     6361 ctgctttttt tgaacaaaat ggttttaaaa ctcctgaaat tttaaactct tctgaaatta
     6421 caaaatttcc tgtatttata aaacctctga atggtagttc atctgttaac acatttattg
     6481 taaataatga aaaagaattg ttgtttttta gtgaatatat agataatcct cttattcaag
     6541 agtatatttt aggtgaagaa tatactatag atgtttgctt aaactttgat tcgacaccag
     6601 taagtattgt tcctagaaaa agaatagcaa ctagaggtgg ggaaatactg aagggtcaga
     6661 ttattaaaga tagagaacta attgagacta cgaaaaagat gttggaaaaa ttaaatgcaa
     6721 tcggacatat tactattcaa tgtataaaaa acgaaaaggg aatattttat ttagaaataa
     6781 atgcaagata cggtggagga gcccccatta gcattaaatc aggagctaat tcccctgaat
     6841 atattataaa gatgatagat tcaaaaaaaa atttgagata cagagaaaat tatttagata
     6901 aaatgatagc actgagatat gatcaagcga tttatttaga tgaaaaagga aatgtatata
     6961 atgattaaag cggtgatctt tgatttagat gacactcttg tgtctgaata tgattatgta
     7021 aaaagtggat acaaatgtgt agctagaaga ttagcagtgc aaaaaatttt cccggctact
     7081 tttgacgaaa tatatagagt ccttttagat ctttttgctg aagatagtaa aaatgtattt
     7141 aatcgtctat atgactattt tgaaattcct tatagtaaaa aagaaattaa agaactggta
     7201 aatgtatatc gagaacacaa accagacata aattttttag aaaatgcaga tactttatta
     7261 acggaattaa gaagtagagg atacaagctg gggattatta cagatgggta tgctataacc
     7321 caacgaaaca aattggatgc tttgaaagta gactatttgg tagattatat ttgtattact
     7381 gacgaattag gccgtgaatt gtggaaacca caccctatct catatctttt attgttagaa
     7441 aagtttaatt taaaggcaca agagtgtata tatattggcg ataatgagtt gaaagatttt
     7501 ataactgcca aatcaatagg aatgcaaaca attaaaatag aaagagtaaa tggtatttac
     7561 agagaaagca gtttgaagtt caaaagattt taccaagctg ataaaaaaat acgttcgtta
     7621 tgggagataa aaaatttttt gggagtgatt tcatgaaaag taaaagaatt ctattagctt
     7681 ctccacatat gagtgaagaa ggatatgaaa aagagtatgt aaaagaagca tttgatacaa
     7741 attgggttgc tcctttaggt gcgaatgtga atggatttga gaaagaatta gcagaatacg
     7801 tgggagctag tcatgctgcc gcattatctt ctggaacagc agctatacat ttagctttga
     7861 aagcggctgg ggtcggtaaa gatgacatag tgatttgtca gtccttgacg ttctcagcta
     7921 ctgctaatcc aattctctat caaggagcta tacctgtatt tgttgatagt gattacgaaa
     7981 cttggaatat gaaacctgaa catttgcgaa cagcactaga aaaatatcct aacacaaagg
     8041 ctgtattagt agtacatttg tacggtttat cagctaattt agacgaaatt actaaaatat
     8101 gcaaagaata cgatgttact ctaattgaag atgctgcaga atcattagga acaacatata
     8161 aaggaaaata tactggtaca tttggcgact atggaattta ttcttttaat ggaaataaga
     8221 taatcacaac ttcaggtggc ggaatgcttg tatccaatga caaagaaaag gtggaaaaag
     8281 tgagattttg ggctacgcaa tctcgtgaga aggcacgcca ttatcaacac aatgaaattg
     8341 gctataatta tcgaatgagc aatatcgttg ctggtatcgg tcgaggacaa ttgaaagtat
     8401 taaatgaaag aattgagaaa aaacgatata ttcatagttt ttataaaaaa gaattgtcta
     8461 aattgtcaaa tatacaattc atgccagaaa ataattggaa tgaaccgaat tattggttaa
     8521 gtgtcataac aataaaggga ataaatgcga atcatcttat tgatgagtta gacaaaaata
     8581 atatagaagc ccgcccagta tggaaaccta tgcatttaca acctgttttt aaaaattatg
     8641 attatattgg cgatgatgta gcagaaactt tatttgactt aggagtatgt ttaccatcag
     8701 atacaaagat gacagatgaa gatttaaaat atgtagtatc tacgattaaa agagtgatca
     8761 atgatgcgta aaaaaacttt ctatcagctt tttgggaaaa gaattttaga tatcctattg
     8821 tcaggaattg ctctcatcgt tttatcgccg ataatcttaa ttgtaggttt tttagtaaga
     8881 attaaacttg gttctccaat tatcttcaaa caagaacgac caggaaaaag tgaaaaaatt
     8941 ttttccatgt ataaatttcg tacaatgact gacgaaagag accacaatgg agaatattta
     9001 cctgatgaaa ttcgactaac taaatttggg aaaatgctta gagcaacaag tctggatgag
     9061 ctacccgaac tttggaatat tttgaaaggt gatatgtcga tcgtaggacc tcgtccatta
     9121 ttagtggagt atttgccact atatagtgag aagcaacgga aaagacacaa cgtaagacca
     9181 ggattaactg gactggctca ggtcaatgga agaaatgcta ttagttggga agaaaagttt
     9241 gatttagatg tttattatgt tgataaaatt agtttcttta atgatcttat tattataatt
     9301 cagacatgta aaaaagtaat caagaaagag aatattaata caatcaataa caatatccca
     9361 gaaaaattta aagggagtta aatacagcag cttaatgcaa gttttgtgac ttgcattata
     9421 tttaggtata aatcaaagcg agtatattag aattatataa catatgttaa aaaaacgtat
     9481 agctaataaa agtatgattt taaaaatttg tgtagagttt taaatatact gtttcaatac
     9541 gagaaaatta taaaaaagta taaggaaaag agagttatgg caaagtatta taataaaaaa
     9601 attcttataa tggcaaacaa cgatgatggt ctttaccgtt ttcgaggaat gctcataaaa
     9661 aacttagtag atcaaggtaa tgaagttatt gtatctcttc caaagggcaa gaatattcct
     9721 aaattaattg aacttggatg tcaaattatt gaaactccaa tagatagaag aggaattaat
     9781 ccaaaaactg actttaaact attttttaac tattgtaata ttttgaaaaa aataaagcca
     9841 gatatggtca ttgcttatac tatcaaacca aatatatacg gtggattggc atgtcggtta
     9901 agaaaggttt cttatgcatt aaatataacc ggattaggaa cggcatttca aaaaaagggg
     9961 gttttgaaaa gtcttattgt atttttgtat agattagcag ctaaaaaatc taaggtcata
    10021 tttttcgaaa attttgaaaa tatgaaaaca tttcagactt tacaaattgc taaacaaagt
    10081 aaatacaagc tattaaacgg tgcaggagta gatgtagaac actttagtta tttagaatat
    10141 cctaaagatg agcaagttca ttttttattt attggaagaa ttatgaaaga gaaaggtgta
    10201 gatgaacttt ttgaagcaat gaaaaactta tatagtgaag agaaaaattg tgtacttgat
    10261 gtccttgggt attttgaaga agattacggc ataaaaattg aaaaatacga aaaagaggga
    10321 tggttaaaat atcatggata tcaatctgat attcgtccct ttgtagaaag agcacattgc
    10381 tttgttttgc ctagttggca cgaaggaatg gcaaatacta accttgaatc agcagcatca
    10441 gggagaccaa ttattacaag tgatatccca gggtgtaaag aagcagttat tgaatcgaag
    10501 tctggttatt tatgtgaaag aaaaaatagt tgtagtctct ataattcgat gaataaattt
    10561 ctaaaattat catatgagca gcgcattaac atgggaaaaa tggggagaaa ttatatgatt
    10621 aaaaaattta ataagaagaa agttgtcgat gaaacaatta aatcattatt tagttaaaaa
    10681 gatatgtttt gcaaagtatg tgatagaaaa gagggattct gtggcttcag aatagatatc
    10741 aaagaattgg caaaagtttc tgatattttt ttatttacta cattacaaga aggaatgcca
    10801 agatctatga tggaagctat ggcaagtgga ttgccatgta tagcaagtaa aatccgaggt
    10861 aatgtagacc ttttagtgga aggaaaaggt ggatatcttg ttccagctaa agatgcagaa
    10921 ttagtagcag agtgtttgag agaactaatc aataataaat ctcttagaaa gtatatggga
    10981 aatgagaacc tttccagaat taaagaattc gaggttagta tcgtaaaaaa aagaatcaaa
    11041 gaaatatatg aagaagttat aggtgagagt gtaaaatgaa aaatccaatg ttttcaataa
    11101 tagtacctat ctattgcgtt gaaaaatatt tgaaagattg cttagattca ataagaaatc
    11161 agacatttaa aaattttgaa gcaatactag ttgatgacgg tagtcctgac gaatgcccag
    11221 aaatatgtga ctattattcc caaatagatg gacgtttaaa agtaattcat aagaaaaacg
    11281 gtggaataat agatgcacgg aaaacaggag tcactagtgc tatgggagag tatatagttt
    11341 ttctagatgg tgatgattgg cttgcaaata cttatttaga aaaatatgcg gaagtaatta
    11401 ctaagtacaa tccagatata atatgtagtg ggtattttgc atcacgttcc aacaaaatta
    11461 gaaaaagaga attgtcttgc tcgtttggat attatgacaa agataaaatt ataaatttga
    11521 tttatcctaa attaattgag gatgaacatg gagagtattt tagaccacaa ttatgggcaa
    11581 aagcttttag aactactctg tacaaggaac aacaacaagt atctgggcaa gttagtgttg
    11641 gtgaagatca tgcatgtaca aaaccatgta tttaccatgc aaattcgata tatatgatgg
    11701 aaaaatgttt atattattac agaataaacg aaaattctat aacaaaaaaa ccacaaccat
    11761 attattggga aggaccaaag gctattggag agcattttga aaagcagatt gatatgaata
    11821 agtatgattt tcaagatcaa gtatatagaa atgttacaca caatttattt aatgttgctg
    11881 tttcacaatt taataaacag gacaaatatt taaatataaa aaaagatatt atacaaaatc
    11941 taagtgacga atactatact agagctattt gt
//
LOCUS       DAZUMI010000096        11903 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000096 DAZUMI010000000
VERSION     DAZUMI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.097"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..294)
                     /locus_tag="ACG81C_002178"
     CDS             complement(88..294)
                     /locus_tag="ACG81C_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065323.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(310..924)
                     /locus_tag="ACG81C_002179"
     CDS             complement(310..924)
                     /locus_tag="ACG81C_002179"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5065324.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1071..1754)
                     /locus_tag="ACG81C_002180"
     CDS             complement(1071..1754)
                     /locus_tag="ACG81C_002180"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5065325.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1863..2321)
                     /locus_tag="ACG81C_002181"
     CDS             complement(1863..2321)
                     /locus_tag="ACG81C_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5065326.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2405..4168)
                     /locus_tag="ACG81C_002182"
     CDS             complement(2405..4168)
                     /locus_tag="ACG81C_002182"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5065327.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4389..5849)
                     /locus_tag="ACG81C_002183"
     CDS             complement(4389..5849)
                     /locus_tag="ACG81C_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065328.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6057..6983)
                     /gene="glsA"
                     /locus_tag="ACG81C_002184"
     CDS             complement(6057..6983)
                     /gene="glsA"
                     /locus_tag="ACG81C_002184"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5065329.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7006..8433)
                     /locus_tag="ACG81C_002185"
     CDS             complement(7006..8433)
                     /locus_tag="ACG81C_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5065330.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8464..9678)
                     /locus_tag="ACG81C_002186"
     CDS             complement(8464..9678)
                     /locus_tag="ACG81C_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5065331.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9764..9991)
                     /locus_tag="ACG81C_002187"
     CDS             complement(9764..9991)
                     /locus_tag="ACG81C_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065332.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(10019..10261)
                     /locus_tag="ACG81C_002188"
     CDS             complement(10019..10261)
                     /locus_tag="ACG81C_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5065333.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10293..10850)
                     /locus_tag="ACG81C_002189"
     CDS             complement(10293..10850)
                     /locus_tag="ACG81C_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5065334.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10863..11051)
                     /locus_tag="ACG81C_002190"
     CDS             complement(10863..11051)
                     /locus_tag="ACG81C_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5065335.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11064..11630)
                     /gene="amaP"
                     /locus_tag="ACG81C_002191"
     CDS             complement(11064..11630)
                     /gene="amaP"
                     /locus_tag="ACG81C_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5065336.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3485 a         2456 c         1945 g         4017 t
ORIGIN      
        1 aattctttca gttgcaccag gattctttga taatttttta gttttatgtg tttttttata
       61 tgttttttca cttttcggaa taaatattta tttgttaaag taattaaaca atccgctaat
      121 cgtttgatct ggcatttcta tttccttacc atctaaagta acagtagtaa cgacaaaatg
      181 gtcttgcaat ggcggaactt ctaagtactg cccttgcaga aaatcattca ctgtttcata
      241 ttcttttttc atttcttttg aatctgtcga tggtttatag cttattttta acataactag
      301 tgctcctttt tattcgggac gtgcaatcgt gaaaatttct cctattgctg cataatcatt
      361 tcctgccaaa cggcttacag cattcaattt tcttggatcg atccttcctg tcttatcatc
      421 atagactgtt tcatttaaat gatatttctg tacttctagc aaaaataaat cagctgttac
      481 tttattttta ttccgtatct cgacatgttc atataaggat gtttcaaaac gaacttttgc
      541 ttctgtcaat gctggtactg aaatgattac tgaatcagtt aaagtcaagt cagtcaggta
      601 cagttcgctt tcctcattag gcagattggc tgctgtttta tttacttctt ctacgttgtc
      661 ctcatcaagg atatggataa cagcttctct tgtctcaaga atattctttg ctgtgtcttt
      721 tactgcatct tgttttctct ggatcgccaa ggataagatg ggtcgattcg aactgacgat
      781 attgaagtaa ctgaagggtg cgatattcac tatatctttt gctgatttag tcgttactac
      841 tgctactggt ctagggatga ctgaaccaat cagcaacttg taattttcac gttctgtcaa
      901 ttctgaagga ctccgttcaa acatggtttc gccttctttc tataaaattc gttaaaaagc
      961 agatcttgaa gacaaaatga aaagtaatgc ttttttcatc ttcttagaat ctgctttaaa
     1021 ctatctatca atttttcttt tttgtcaaga atctgtgccg tgcgtgtttt ttagttattt
     1081 gctttacttg cggcttcgac atatctttta gtgatcaatt gcggacgggt gataatagat
     1141 ccaactacga ctgaaaaagc acctaattca tatgctttct ctacttgttc aggagagtca
     1201 gtatgtcctt ccataattac tgggatatcg acttgttcta tgatttgatt caataaatca
     1261 aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc ctgctaaagt cgtagacaaa
     1321 agatcaaaac catcttcttg cgcttgaagc gcttcctcaa aagtcgaaat atctgccata
     1381 gctaaccgac cgtgttcgtg aatcatttgg acaagttcag cagttgtttt ttctgtcgga
     1441 cgtttacgag aagttgcatc cagtgcaatg atttctgctt gcgtctccaa aagttcttcc
     1501 acttcttttt ctgtcgcagt aataaacact tctgacccat cgtaatctct tttgataatc
     1561 cctattaccg gtaaatcaac gattcgcttg atttcattga tatcttctgt cgtatttgct
     1621 cgaataccaa ctgctccgcc ttcttttgca gccaaagcca ttctccccat aataaaagaa
     1681 ctgtgtagcg gctcgttttc caaagcttga caagaaacga cgactccgcc tttgatttgt
     1741 tccagtgtat ccacaatatc tcctccagta atgttatata aaaatacagc ttcatataaa
     1801 aatgtaaccc ttttctattc aggagacaat aatgagctat ttctttcagc agacctttct
     1861 tactaaaaga aaattacttc aaattcttcg tatacgacca gcttctcttc tgtaaattct
     1921 atgttcaacg cttctttata agcatgttta aaaaactcag catggacttt ccgccaataa
     1981 gctaatgtcc gatccccttc tccttctttc caagcatgct ctttcgtgac ttcactaaaa
     2041 ggcaagatgg tcacatttat atttttgatg atacaaactg gttcattttt tccattcagg
     2101 accacgtcta attgaccaac ttttggtaaa ggctcattct cttgtttata cagatcgtaa
     2161 ccacttgatg taccagtctt tatctttttt acaactagtt cgctcaactc gtctgccatt
     2221 ttttctgtat ccccaaatga aaagaaacct aatttttctg attgataact aggatatttt
     2281 tcttggaact ttttgacata ttcttctact tgctgataca taacatccac ccctttgctt
     2341 tttataatga aaaaaccatc cagatactag tataaacaat tatctggatg gtgagcataa
     2401 tgaattattg aatattcaag acacgcagac tatcttcaac gatttgtttt ggtgttaaat
     2461 gataacgttc ataaagctgc cctacaggaa cgctatccgt gaattctttc tctgcaccaa
     2521 aatttaatac gttcattgaa gaatcaccat aataacgact gatattttcg ccaaatcctc
     2581 ctgacaaact accatcttct aatgtgatga ctaattcgtg tgtttctttt aatttatcta
     2641 atgtttctac gtcattttct gtgatcgttc ctggattgat caacgttgct tcgattcctt
     2701 tttttctaaa agcttttact gtttcttttc ctaattcaag aaaaccgcct aaagctaaaa
     2761 ttgctacttt tcctccatta tttactgttt gataggctgg aatactataa tcatttgcga
     2821 tagctgtacc atgttctaca ccatgtgtag gaattcggat tgctacaggc tgcgctgttt
     2881 gttcgaacgc ccaatctagc atagagatca tatcctcttt tgttgctgga gcaagatatt
     2941 tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt tccttggtgt gttcggtcac
     3001 tagctgagat cgttccgcca ccgactaaaa taatcactgg attctcgttg atcgctaaat
     3061 catgtgataa ttgatcatat gcccgttgca agaaagtact tgattggaag acgactggac
     3121 gtactccctg agaagccaaa gcagaagcga aagtgataga gtgctgttca gcgatcccca
     3181 cgtcaaaata atgatctgga tgtttttctt ggaatgtttt cagtccaaag gcgcctggta
     3241 tcgctgcatt gactgcggca ataggagctg tacttgtttg cattttctta tccaaataat
     3301 ccatgatgat catattaaat gtttctccag aagttacttg ctttgcctcc ccggtggaca
     3361 gatcaaatgg tacatgccaa tgatatttca ttttattatc gattgccggc tgataaccgc
     3421 gacctttttc cgtatggacg tgaagtacaa tcggatgatc aatatctttt acttcttcaa
     3481 ataaacggat caccgtttcc agatcgtttc cattatccac gtaacgataa tcaaatccca
     3541 ttgctttaaa taggttattg ctgcttttac cgttggtttg acgaagctca gctaagtttt
     3601 tatataaact gccatgattc tcatcaatag acatctcatt atcgtttact aatacgatta
     3661 gattggattg taaaacacca ccattgttca atccttcaaa agctaagcca ccacttaggg
     3721 agccgtcacc gataaatgct accacatttc ctgattcttt cttcagatct ctcgctttag
     3781 ctaaacctgt tgctaagctg atagatgtag atgtgtgccc cactgtaaaa tagtcatgtt
     3841 tactttcatg ttggttggaa tatccgctca cttcacggta atgtcctttt tcaaaaccat
     3901 tttttcgacc agttaaaagc ttatgaccat aagactgatg ggaaacatcc caaacgattt
     3961 tatcctttgg tgaatcaaat acataatgaa acgcaatcgt taattctact acacctagat
     4021 tcggaccaac gtgcccacct acatcgctgt ccttttgcaa gattttttgg cgcatctctt
     4081 ctgccaactg tttcatttcc tcaacagata attttttgac atctgcgggg ctattgattg
     4141 catccaatac atgtaatact gtactcataa tacattctct cctccgtact atcttctttt
     4201 aatgaaaata gatttgttac actttatcta ttctactact tagagttgac tctaagtcaa
     4261 tagcatttta aaaaaatttc agagaaagct tgttatataa gtagatcttc gcacgaatca
     4321 ggactcaaat aaaaaccttc tacagacaat taaaaagctt tgtagaaggt ctcctaatta
     4381 aggtctcatc aagaaatatg tttttttaat aggtgaagtt cctgagcaat atgatacaaa
     4441 tggatgtttg caccttttaa tgagattgga tagatctttg tcttttcagt ttctaatcca
     4501 ttttgatagc tagcagcatc gcacaaaacc actctttttt tcgcacatag acattcatca
     4561 ggcaattctt ctaatgaacg aaataaaata ggatttattt cagcgatgcc ttcttccgca
     4621 tacaaatgaa attgggttcg ataaaaaaat tgtttgactg aactttctga aacaacaaaa
     4681 atttctattt cctgtctccc cgcttgttca ctgttgatca aagaaagact tttgacgaat
     4741 tttcgaatga aattttcatt ccatcttagt tggatttgat ttttcttttt ccactcttcc
     4801 aatatttcta caatttttcc gtacagaagt tgttcttttt ctgatagaag atagatcctt
     4861 tccggtaaaa atacttgtcc atctgcccac aacgtacgga caaattgtaa aaaggattgc
     4921 ttgaaaataa aattttctgc atcgtcgatt tcagcgatac taataatctg taaaatcaat
     4981 tcattcagtg ataggtcatt gagaacaaaa tttttataaa caacttcgaa atcttttctt
     5041 aacagctcca tgttctcatt ggtataattt ctactgttga acaaagaaaa aatataatag
     5101 acatcttctt cttgtaagtc tagaattcct ttttgctctt ccaatccttg acagactaat
     5161 tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat
     5221 gcctttcttc cgattgcgat ctctattcct cgtatgataa aggacttttc atcttccgaa
     5281 tatcttcttt taaaaaaatg agtctctaca taatcaatca actcgttaca agcttcttct
     5341 acatacggat ttttattttc atagccttcc caattgacat aacgtactaa agctagcaag
     5401 atgttccgcg agtcaaattc tgggataatg atttctttat ttttcaaata atcattttct
     5461 ttaaaaaaat ccaatacgat ttttttgatt gtatatactt tgctcaaaga aataaattca
     5521 agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa aaactttaaa
     5581 aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc atgtttgaat
     5641 acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc tagacactcg
     5701 gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga aatagctgtt
     5761 tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac ttttctaaat
     5821 atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt tttcttatga
     5881 ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag aaaagccctt
     5941 tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca atttcagcct
     6001 cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa gaatggttat
     6061 tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat accagctgcg
     6121 ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg atttggcaca
     6181 gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg gacggaaaac
     6241 tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat tgtagcactt
     6301 tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc cagcacagca
     6361 gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa ataagcatcg
     6421 agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta agcaagagag
     6481 cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa tgtgatatta
     6541 ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc atctgcatct
     6601 ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg attcattgga
     6661 tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc ggttggttcc
     6721 gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa taacaatacg
     6781 ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt
     6841 tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac ttcagctaat
     6901 gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt tccaatcaat
     6961 tgttctaatt cttttacttc catttgaata accagctttc attttttatt tttctgcatg
     7021 ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga taagataaga
     7081 aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag caggtggaac
     7141 aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa cgattacttt
     7201 tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt taaaaaccag
     7261 cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa cagtcaatga
     7321 catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga cggctgccca
     7381 gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc
     7441 ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg cttttgaagg
     7501 agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa tcgcaagaat
     7561 tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta acgattgaat
     7621 cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg tcaatccgcc
     7681 aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg gataatcctt
     7741 tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca tatatgctaa
     7801 aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt
     7861 catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa
     7921 caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa tcaatgaagt
     7981 gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca ataggattgc
     8041 caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg ataatgcacc
     8101 agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat
     8161 tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga caccgccttt
     8221 ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga taaaccatag
     8281 caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga atgtgggata
     8341 ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga acgttaattt
     8401 ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct atttgaattt
     8461 gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt gttcttcttt
     8521 tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg tcagcacata
     8581 ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt accagctgct
     8641 tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg tacctgtgaa
     8701 tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt tctttttacg
     8761 aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca tggcactttt
     8821 tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg atcctgttag
     8881 tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc aagtaatcat
     8941 cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt catctgctcg
     9001 aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg taccgatagc
     9061 agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc tttgaccaag
     9121 tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg ctccaccagc
     9181 gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt ggcaaacagg
     9241 gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta aattcaatct
     9301 ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca gttggtaaag
     9361 aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg
     9421 tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc gtccaaaggc
     9481 caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga gaaaagattg
     9541 agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa aagctagacc
     9601 aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt
     9661 gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat aaaagaaggt
     9721 gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa agacagacaa
     9781 ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa
     9841 taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttttatt tttaaagata
     9901 ttttttcata aggaataaag aatacttcat ataaaatata taaaaaagaa ccgcagcgag
     9961 tgtcgctacg gttctcaaat ttgttcttca tttcaacata agtttttctt caatttgctt
    10021 ataatttacg tgcaaagaat gaaacgacta atactaaaat gattgcaccg atcaatgatg
    10081 gaatgatagc cattcctgca attgttggtc cccaagttcc tagtaaagct tgtccaattg
    10141 ctgaacctac taaaccagca atgatgtttg cgatgatccc cattgatgat cctttattcg
    10201 tgattgctcc tgcaattgct ccaatgatac cgccgacgat taatgcccaa ataaatccca
    10261 ttttaaattc ctcctaactg acttcttact tcttattgta cacgtggttc gttattttct
    10321 ttgacttttt gtgcaccttt gcctacagca gattttgctt tatctgtttg gcgtgaagca
    10381 aattgtcctg tggattttgc tgcttctgtt actttgtctt gtactgtttc ttgatctttt
    10441 tggtattctt cttgtgtttt gatgtctgca acattcacat tcacttcgat aacatctaaa
    10501 tgtgtcatat tgtttacttc agtactgatc aattctttga tttggttgta aatatcttcg
    10561 atgtctttac cgtattctac gatcacatct agatccacag ctacttgttt cttacctact
    10621 tctgtattga tacctgctgt tacgttatct gtgttgacta atttttcagc catatttgag
    10681 aaaaatccgc catctaatgt caatagacca tctacatctt tcaatgcata accgatgatt
    10741 ttttggatga ctttgtcttc ataagtcaat tcaccattca tcatgcggtt ttctcctttt
    10801 tcatttgcca aaactgatcc atttgttgta ttcatgtttt tgttttccat ctttgtttcc
    10861 tcctatgatt taaaaaattg atcaactatt cctgactgtt tcaaataaaa accgattcct
    10921 gcgccaattg ctgtcatgat tatgatcaac aatgtcttga aaaatccaat cgttaaccat
    10981 aaaacagcta gcagtagtcc aagaccccca aagataatag ggagcttgta tgctgaaaat
    11041 agttctttca ttatttggcc ctcctattcc actcgagagt gcttgtagtc tcttgtatct
    11101 tcttgttctt cataaccgct gtatgtgact gccactttga ccgtttcttt tgttccgagt
    11161 actttagtta cttcttcttc gatatcttgc atcaatacac ctgttttccc tactaatgaa
    11221 gaagttcgtt tcaagtctcc gttgacatgg atatggattc gatttttcgt tgaacgtatc
    11281 cgtactttag gtgaatgaat gaactcttct tcgtgtaaat gggaacgtac caacccttcg
    11341 atcgcttttt tgtcgattgt tagtttgcca tcttcccgtt tcataacgaa agttcctctt
    11401 gactttggat agaaaagaac gactagcaag atgataatag caagaacggc aaatactaca
    11461 gctgcccaga aaatatattg ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt
    11521 tccaattgtt caggcataat atacggcgca ttgatcaacg caatcgctag aaatactgaa
    11581 agtagcagta aactcatgat aacaccgatt atttttaacc ccttattcat tttccacctc
    11641 ctataaactt atttttaccc tgtaaataac tctaccgaca atcacagtat agatagcgaa
    11701 cagttagcat agcatcagta aataaagcgc taccaaacat tgctggatca taacggcgtt
    11761 ttattgccag tctgatactg aaataacaat tgcttactta ttctctattt acttttgatt
    11821 gctggctata cttaaaggat attattcaag attaaaaatt gcaaagatta tgctcagtta
    11881 ttattacaag attctattta ttt
//
LOCUS       DAZUMI010000097        11820 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000097 DAZUMI010000000
VERSION     DAZUMI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.098"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(305..1474)
                     /locus_tag="ACG81C_002192"
     CDS             complement(305..1474)
                     /locus_tag="ACG81C_002192"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5065337.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1678..2853
                     /gene="mltG"
                     /locus_tag="ACG81C_002193"
     CDS             1678..2853
                     /gene="mltG"
                     /locus_tag="ACG81C_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5065338.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2989..3468
                     /gene="greA"
                     /locus_tag="ACG81C_002194"
     CDS             2989..3468
                     /gene="greA"
                     /locus_tag="ACG81C_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5065339.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3637..4368
                     /gene="liaF"
                     /locus_tag="ACG81C_002195"
     CDS             3637..4368
                     /gene="liaF"
                     /locus_tag="ACG81C_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5065340.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4365..5432
                     /gene="liaS"
                     /locus_tag="ACG81C_002196"
     CDS             4365..5432
                     /gene="liaS"
                     /locus_tag="ACG81C_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5065341.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5439..6071
                     /locus_tag="ACG81C_002197"
     CDS             5439..6071
                     /locus_tag="ACG81C_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5065342.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6211..6876
                     /locus_tag="ACG81C_002198"
     CDS             6211..6876
                     /locus_tag="ACG81C_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5065343.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6904..7197
                     /locus_tag="ACG81C_002199"
     CDS             6904..7197
                     /locus_tag="ACG81C_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5065344.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7265..8212)
                     /locus_tag="ACG81C_002200"
     CDS             complement(7265..8212)
                     /locus_tag="ACG81C_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5065345.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8390..9193)
                     /locus_tag="ACG81C_002201"
     CDS             complement(8390..9193)
                     /locus_tag="ACG81C_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5065346.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9339..10253
                     /locus_tag="ACG81C_002202"
     CDS             9339..10253
                     /locus_tag="ACG81C_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5065347.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10301..10771
                     /locus_tag="ACG81C_002203"
     CDS             10301..10771
                     /locus_tag="ACG81C_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5065348.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10856..11464
                     /locus_tag="ACG81C_002204"
     CDS             10856..11464
                     /locus_tag="ACG81C_002204"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5065349.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11598..11798
                     /locus_tag="ACG81C_002205"
     CDS             11598..11798
                     /locus_tag="ACG81C_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5065350.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         3980 a         1823 c         2380 g         3637 t
ORIGIN      
        1 attgcataaa attaaaaata agaacaagcc atgaatgatt tgctagcaca tattttagca
       61 atctaaagat actattttag atttaatagg ccctttgtca aataatgttg gtaaagagaa
      121 atcttagaat aatttggaca gaacagaaag aagaacacct ctttctgttc tgtttatttg
      181 gaattaaagt ctgtagcaaa aattgatttt tagatttgtc ccacactctt tcctacgttt
      241 agtcgagtac tttttttaag caaatgctat tcaaagaagt agtatttgct atcttttcat
      301 catatcagct atttgccaac ttagtagttc cttccatcag gtcatagatt tggtttctta
      361 gatcagcagt aggcagtttg atagttttgt cagcttttat attgctttcc atgatgatag
      421 catcctgtga atctttgcta aatatagcag atgtatcaaa agagtcgata tttccttgcg
      481 acaggatata attatctccg ctatataccc caccactata tggatgtcca ttcgcatttt
      541 cacgccataa tgagaagagt aagtctttgg aaactagttt tccagataaa acttgtgtaa
      601 acagcgtatt taaatcatag acggacatac tgatattacc agtccctaat tcgttatgga
      661 tctctgtttc agattctgtc agtggtttaa agtaatcatc ctctttagac atttgatagg
      721 atagtgcatg attggatgaa tttttgactt ggtcataaaa ttttgtatgc tgtaatccta
      781 aaggttcgat gatatcatcg gtcaataatt tttcatacgg ttgatttgtt aatttgatca
      841 aaattccagt caatatggta aagttcaatg aatcataatg agaggtctga taatctgtaa
      901 attttaagtg ctgaatcgca aattgaacga cttcttcatc agtcatcggt atggttggag
      961 aagcagttcg gtgcaagcct gagcgcatat acagcaagtc acggatagta atttgctggc
     1021 tgccagcaat ttgtggatag aattgagcca gcttagtatc taaagaaagg tgcccacgct
     1081 cgacttgttt taaaatcaaa agagtagtca atgctttttg aaccgaacca atctgataat
     1141 aaagagaagg atcatttggt tgttgcgtga gtttattcgc atagccatag cctttttcca
     1201 tgatgatgcg attatttttc tttacgtaaa ttgagccaat ataatcattt tttcttaata
     1261 gttgatcgat tttttcaacg agctgttgtt cattggtagt cagtatcaac tttttttgag
     1321 ctgattgagc atgggcaact atctgagagt gttcattatt gtaaaattgc catccaagaa
     1381 tagttcctgt agagaacgca ctaaggctaa acaacacgac aacgatcatt aaaaatgaat
     1441 attttctttt tttagcatgt tttccttttt tcataatagt ttatttagtc tcctttcttt
     1501 tttacagaaa gaataaagct aaaatagctt gtatcttgat aacatttatt ttaatcttat
     1561 agtaatggat ttactactta catttttgat agaatattta aataaaatca ttcacgccaa
     1621 ttcaaatatc atgtataatg aataaagtta cgaacttgat agtaaggagg agttgttttg
     1681 tcaggaaaca aacaacaccc aagtaaagat aaaaaagaag ccagtaaggc agctacagca
     1741 gatataatcg acaagcggag aaatctgcgt aaaaaagaag ataagattgt gaggaagatc
     1801 attttagtga ttgctcttac attattgatt attggcggat ttcttggatt taccgtgtat
     1861 cgttatgtag atagcgggtt gaagccttta gataaatcag atgaccaatt agtccaagtc
     1921 gagatcccga gtggctcttc taacaagcag attggtgaga ttttggagaa ggacaatatt
     1981 attaaaagcg ggattgtttt caattattat acaaaattta aaaatttaac tggctttcaa
     2041 gctggctatt atcagttagc gcctaatatg actcttgacg agattggcaa acagttgcaa
     2101 gaaggcggta cttctgagcc aacaaaagtt gcagatggga aaattgccat tccagaaggg
     2161 tacgatatcg atcagattgc tgaacgggta gcgaaggtaa ctggtaaaga caagaaagaa
     2221 tttttagatt tagtgaatga tgagactttc tttaacgagt tgcaccaaaa attcccagaa
     2281 ctgttggaaa gtgcatctaa agctgagaac acaaaatacc gtttggaagg ttacttattc
     2341 ccagccacat atgattatta cacgagtact tcgcttaagg atctagtgat tgaaatggtg
     2401 aacaagacga atacggtcat gcaaaactat tattcagcta tcaagcagaa aaatttgact
     2461 gtacaagaag ttttaactct agcttctcta gttgaaaaag aaggcgtaaa agaaaatgat
     2521 cgtaaaaaca ttgcacaagt cttctttaac cgaatcaaag caaatatgcc acttcagtca
     2581 gatatctctg ttttatacgc attaggtgaa cacaaagaat tagtgacgta tgaagataca
     2641 gctgtagatt caccatataa tttgtatacc aataccggct atggacctgg accttttgac
     2701 aatccaagcg aagaggcaat caaggcagta ttagaaccag cagaaaatga ctattattat
     2761 ttcgtagcag atacgagcac tggaaatgtt tatttcgcta aaacttatga agaacatatg
     2821 gaattagtgg aaaaatatgt aaataattct tagaaaaaca ggctctagac attgatcatt
     2881 tttagaaaaa catttacatt tgaagaaaag cgtggtaatc ttttttggac tatattcctc
     2941 aaaagattac cttttgcggt attttatcga aaaaaggaga cgttacacat ggtagagaaa
     3001 gtttatccaa tgactctaga aggtaaagaa aaattagaaa aagaattaga agaactaaaa
     3061 acagtcaaac gcggagaaat cattgaacga atcaaaattg cccgcggttt cggagatctt
     3121 tccgaaaact ctgaatatga atcagcgaaa gatgaacaag catttgttga aggaagaatc
     3181 acaacaattg aaaatatgat tcgttttgct caaatcatcg ataacgacgg tgtagatgca
     3241 gatgaggtat caattggtaa aacagttaca ttcatcgaac taccagatgg agaagaagaa
     3301 gagtacacga ttgtcggaag cgctgaagca gatccgtttt ctggcaaaat ttccaatgat
     3361 tctcctattg cgcaagcatt gattggcaaa cgtaaaaatg atgaagtaac tattgctact
     3421 ccaggtggcg atatgttagt taaaatcgtc aaagtagaac aagcgtagtc tgactgaaag
     3481 atctagagag ttcatcttac tgatgataca ctctagatct ttttctcttt gtgtatttta
     3541 gatttcaaca aacgatctta catctaaagt cctatagcca acagttaaaa aaataggtat
     3601 gatgaaatga gaatgtgtca ggagggagaa agagcgatga atagttcttg gcgttttttt
     3661 gtggtagttg aagcactact gctgttattt gctgtttggc aaatcgtaaa caatactgga
     3721 cttttgcttt tagtgatatt cggtattttc aatatatatc tagctatgcg caaacatcct
     3781 cgtactaaat ttcaaaattt tcaattgata ttaggaagtt tggtcatttt ttttagctta
     3841 gtcaacagtc ctgctttatg gatgatggct gtatttgccg ttttatttat tggtctgaaa
     3901 ggtgtagaga tttcagggat agatttaacc aaaaatgctt tttggcgaaa gaaacagatc
     3961 atgatgatcc aaacagagca attaaaaaca cataataatg agcggaaaag acagcaatta
     4021 ttcggtaacc aacgtattgg aaacgacgtc tatgaatggg acgatatcaa catcgccgtc
     4081 atttctggtg atactatcat agatttgggc aatacgcttt tgccaaaaga tgataatatt
     4141 gtgattgtaa gaaaagggat aggcagaaca cggatcttag tacctttggg cgtagccatc
     4201 cgattagagc atgcgacatt agtaggaaat gtcttgtttg aagaagagca attttctttg
     4261 aaaaatgaac aaatcaaaat ctacagcagt gattatgatg aaaaccctcg tagattgaag
     4321 attattacta ataccttgat tggagatgtc gaggtgatac gaatatgatg ggaaaaatat
     4381 ccagagcgat gctagctgtt tattcgggga ttgcggcttt ccttattatt ctattttcac
     4441 ttttcactta tttttatgcc agcaatcaaa gtcattggtg gggagaatta ctgcgtgcac
     4501 gtttattata tgttccgctt atcttccatt tgttagccat ttccttaggt gtaggattaa
     4561 ttgtctttct attattatca cttattcaaa agacaaaata cgggaaaatc gaagaaaagc
     4621 tgcgtgcact ttcttctggc aattatgaat ccaaattgtt gcttcttccg atcccaagtg
     4681 catcggacga tttatacatc aaagatattg ataaggaaat cactaagata aaagaaaaaa
     4741 tgattgaagt atctagtgaa ttacaaattg taacgagccg tccccaatat gtagatggac
     4801 aaaccaaaga agaaatttta gaattagaaa gacatcgatt agcccgtgag ctgcatgatt
     4861 cagtttcgca acaattgttt gcagcaatga tgatgatgtc ggcattgacg gagcaagcag
     4921 aaaaaagcga gacaccagaa atgtttcgca agcagttgaa aatggtagca gagatcatca
     4981 acgcttccca gtctgagatg cgtgcgctgc ttcttcatct acgtccagtc aatttagaag
     5041 agaaaagttt aaagcaaggt atcgagcagt tattgaagga attgcaaaat aaaatccaga
     5101 tttcgctgaa atgggatgta gaagatgtaa aactatctag ttccattgag gatcacttgt
     5161 tccgaatcgt tcaagaattg ttatcaaata cattaagaca tgctaaagcc aatgaattag
     5221 aggtatattt gcacaaaata gacaataatc ttttgttacg tattattgac gacggaacag
     5281 ggtttaatat gaatgaaacg aaaacgggaa gttatggatt gaacaatatc aaagaacgag
     5341 tagctggtat tggtggaaca gtaaaaatca ttagttttaa aggtcaaggt acaagtgtag
     5401 aaatcaaggt ccctttgatg aaggaggcat agataaggat gataaaagtt ttattagtag
     5461 atgaccatga aatggtgcgc ttaggcgtct ctttttattt atctattcaa gaagacattg
     5521 aagtaattgg agaagctgaa aatggacgac aaggctatga gaaggcgatg acacttcgac
     5581 cggacgtcat tttaatggac ttagtcatgg aagaaatgga tggtatcgaa tcaacaaaag
     5641 caatcttaaa agattggcca gaagctaaaa tcattatcgt aaccagtttt attgatgacg
     5701 aaaaagttta tcctgccatt gaagcaggtg cagcagggta tttgttgaag acctctaccg
     5761 cacatgaaat tgctgatgct ataagggcaa cgcaacgtgg cgagcgagta ttagaaccag
     5821 aagtgacaac aaaaatgatg gaaaaaatga gtcggcgaaa tgagcctgta ttacatgaag
     5881 aactaacgaa tcgggaaaat gaaattttaa tgctgatttc tgaaggaaaa agtaatcagg
     5941 aaatagcgga cgaattgttt attacactga aaacagtaaa aacacatgtt tccaatatat
     6001 tggctaagct agaagtggaa gaccgtacac aagccgctat ctatgcattc aaacatggtt
     6061 tggtgaaata aaaacgatga taaagaggat aatcaaaagc tgagaaaata actattattt
     6121 ttcttggctt ttttacaata atgatgcttg cttttgtttt tgtaaaggct ttgttatact
     6181 gagaaaagaa gatactaaag gaggaaaacg gtgaaacaaa attttgcaat tgtaggtctt
     6241 ggacgatttg gcggaagtat ctgccgaaca ctgatcgaag ctggtcaaga agtactggct
     6301 attgatagta atgaagatcg ggtcaatgaa tatatgaata tagccactca tgctgttgtg
     6361 gcaaatgcac aggatgaaat gacacttcga tcattaggca taagaaattt cgatcatgta
     6421 attgtcgcta ttggagaaga tattcaagcc agtatcttag ttactctgat ggtaaaggaa
     6481 atgggcgtac caaatatttt agcaaaagcc caaaatgagt atcatgccag agtacttgaa
     6541 aagatcggag ctgatcgcgt cgtccatccc gagcgagaca tgggaataag gattgcgcat
     6601 aatttagtgt caaagaatat tttagattac ctagagttgt cagataaata ttctttagca
     6661 gaaatccgag tctccaatcc taaatttttc aacaaaacat tagctgaatt aaatttcagg
     6721 caacgttttg acttaacagt agtcgccata cgaagagtag atcaaacggt tatcgcttct
     6781 cctgcagcag atgaatatgt tcgtgaaaat gataatctac tggttattgg agagacaggc
     6841 gatgtggata ttctagacga taagatgaat caatagaaag aaaagagaaa gtaggttgtt
     6901 gggatgaaat taacggtaac gccaaaagca caaaagtggt ttgactcaga aattgatctg
     6961 ccagaaactt acggaatacg tttttttgga aaagtctacg gcaaaacaga ggttcatgat
     7021 ggtttttcga ttggaatgtc agttgaacag ccagaacatc ctgtaaagaa agtggaaatt
     7081 gatgggacac ttttttttat cgaggaagca gatgaatggt tctttaaagg atatgattta
     7141 cttgttgatt atgatcccaa cctggaggag ccaacctatc actttaaaaa acaataaaaa
     7201 aagagagggt gacacaattt ttgtcacctt ctcttttttc ttttgaaaat attgttttca
     7261 gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg gtggcaaagt
     7321 agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca ggtgtcaaac
     7381 ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga tcttgtggaa
     7441 gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta tttaaagcct
     7501 gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat acagattttg
     7561 gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc gttagaggta
     7621 agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt aaagaaaaag
     7681 tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag ttcatgtcta
     7741 atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg attagtattt
     7801 ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg tcactctgtg
     7861 ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag acatgctccg
     7921 taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc tttgaaaaag
     7981 tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc aaggtagaag
     8041 aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct acattttgta
     8101 gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta tggatttctt
     8161 cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac atagttaatt
     8221 tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag cgccttgctt
     8281 gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa aaatgtcaca
     8341 acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct taattgatcg
     8401 atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca aaagcaatga
     8461 aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt atagtaggca
     8521 atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt gttagtcgaa
     8581 agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc agattcagaa
     8641 aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt atagatgctt
     8701 cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca ttggtgtagg
     8761 gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta ggaagagcta
     8821 tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa tcagtagaga
     8881 tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag tttgtttggt
     8941 aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta aaatcaggaa
     9001 tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga aaaacttgaa
     9061 aagagattgg caaagccata attatactca aaactaaaag atagacaatg gctttccaaa
     9121 atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg ataattaatt
     9181 gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt ctagcgaagt
     9241 atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa tatcaaaaaa
     9301 atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga atagtgccta
     9361 cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat gcggtgatca
     9421 ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt tcagatgctt
     9481 tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa aaaatggccc
     9541 gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata ttgagtgctt
     9601 tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa gcattcaagc
     9661 ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca gttattggat
     9721 tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa agtttgaata
     9781 taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt gtgattatcg
     9841 gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt acactgatca
     9901 tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc gctattttga
     9961 tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa gcaatcgatg
    10021 gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata attattttcg
    10081 acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata tatcctcgta
    10141 taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa gcagagacaa
    10201 aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat tgaaatatag
    10261 agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt atcgacagtt
    10321 aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct tttttggtgg
    10381 tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt ttgatttaag
    10441 tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt ttgtaacgaa
    10501 caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta ttgaatgcgg
    10561 taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa tggtattgtt
    10621 gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa ttattgttat
    10681 ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg tttttgaaga
    10741 agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt tccttctctt
    10801 tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt tttttatgac
    10861 ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata ttgacgaaga
    10921 aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt taaattcagc
    10981 aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat ctgatatgga
    11041 cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac atgctaacca
    11101 ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag gagaaataaa
    11161 agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat tcaaaaaagc
    11221 agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg gaaaattagc
    11281 tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac caattctagg
    11341 tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac cagattatat
    11401 tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg aagcagcaaa
    11461 ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa agagcgaaag
    11521 cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc aagaactttt
    11581 ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat gaaaaaggat
    11641 ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca gcaattcaag
    11701 gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt gttgaaggtg
    11761 cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa taaagacacg
//
LOCUS       DAZUMI010000098        11535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000098 DAZUMI010000000
VERSION     DAZUMI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.099"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..3269
                     /gene="fss3"
                     /locus_tag="ACG81C_002206"
     CDS             42..3269
                     /gene="fss3"
                     /locus_tag="ACG81C_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5065351.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3381..3695
                     /locus_tag="ACG81C_002207"
     CDS             3381..3695
                     /locus_tag="ACG81C_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5065352.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3708..4082
                     /locus_tag="ACG81C_002208"
     CDS             3708..4082
                     /locus_tag="ACG81C_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5065353.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4092..4436
                     /locus_tag="ACG81C_002209"
     CDS             4092..4436
                     /locus_tag="ACG81C_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS5065354.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            4505..5521
                     /locus_tag="ACG81C_002210"
     CDS             4505..5521
                     /locus_tag="ACG81C_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5065355.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6115..7989
                     /gene="ltrA"
                     /locus_tag="ACG81C_002211"
     CDS             6115..7989
                     /gene="ltrA"
                     /locus_tag="ACG81C_002211"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5065356.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8091..8471
                     /locus_tag="ACG81C_002212"
                     /pseudo
     CDS             8091..8471
                     /locus_tag="ACG81C_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            8516..9658
                     /gene="dcm"
                     /locus_tag="ACG81C_002213"
     CDS             8516..9658
                     /gene="dcm"
                     /locus_tag="ACG81C_002213"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFS5065357.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9648..9937
                     /locus_tag="ACG81C_002214"
                     /pseudo
     CDS             9648..9937
                     /locus_tag="ACG81C_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10193..11377
                     /gene="mobT"
                     /locus_tag="ACG81C_002215"
     CDS             10193..11377
                     /gene="mobT"
                     /locus_tag="ACG81C_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5065358.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11374..11487
                     /locus_tag="ACG81C_002216"
     CDS             11374..11487
                     /locus_tag="ACG81C_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5065359.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3864 a         1977 c         2570 g         3124 t
ORIGIN      
        1 catcttattt ttttgtgcct aatttgaaag gagaacattt catgtataaa aaacaaaaaa
       61 agaaattatg tgtattaact attttgagtt tggctgttca atcaattgta ggagcaattg
      121 ctcctacgat tgcttttgct gatgaaatta cccatccaca aacggtaacc gtagaactag
      181 atttagcaca tcaatatgct gttgaaggaa cttttagtga tggtcgtcca atgtcagaag
      241 tcactgttcc gcattatgca gtttacaatg gtgtgaagca agatattttc tgtgttgaac
      301 caggagtccc gatttacaat gagttcactc ctggatatga gaagaacccg ttaccagata
      361 tgtccgataa agccaaatta gtttccgttt tatggaaaaa agtaggtaca gatgcagata
      421 ctcatattgt ggcacagaag atgatctggc aagaagttaa tgggtatacc cttcactcaa
      481 taaaacgatc agatggtagt gccgtaaata tcgcagccat tgaggcaaaa atcaatcaag
      541 ccattgccga ttatcagaag aaaccaagct tccataatag tacggctaaa acagtattag
      601 ggcaatcgac tactgtgacg gatacgaata atctgaattt gtcagagttc gatgaggttg
      661 tggaaaatac cgcaaacatt gattatcgtg tgaatggcaa tcagttgata atcagcccaa
      721 atgccaattc aaaagaaagt ggtgtgttga cgcttaagaa atctgctggt accggaacac
      781 ctgtcgccta taaaaaggct ggccaacaga ctttaatggc cggggcgatc gataagccga
      841 acacttatac ggtcaaaatt gatgtggaaa tggagggttc tttgaagatt aaaaaagttg
      901 ataaagaatc gggtgatatt ataccaggaa cggttttcca tttagacttt ggaaaaactt
      961 tacccgcaaa agacgtgacc accgataaag aaggcattgc gaccttagat gagattcctc
     1021 atggtacaaa ggtaaccatt actgaaaaat cggtgccggc gccttatacg attgatacca
     1081 cacccatgac tgccacaatt aaagcaggtg aaaccatttc agtgacttca aaaaatacgc
     1141 gagaaaaagg tcaaattatt ctggataaaa ccggaattga aacagggagt gatctttgga
     1201 acgataatta ctctttagcg ggcaatacgt ttgccattcg caaggatagc ccaactggtg
     1261 aaatcgtcca agaaatgacc acggatgaaa aaggtcatgc agaaacacca aaagagattg
     1321 ccaatgcgtt ggaattggga acctactatg tgactgaaac caaggctagt catggctttg
     1381 tgaatacttt caaaccagta aaagtcgaat tgaattatgt caatcaaaca gtggctcttg
     1441 ttaccagtaa cgtaaaaggg caaaaccaag aaattactgg tgaaaccact ttgacaaaag
     1501 aagacaaaga taccggtcat gaaactcaag ggaaagctga gtttaaaaga gctgaataca
     1561 ccttgtttac tgttaaggat ggaaaagcgg tcaaatggac cgagaacttt aaaccagaat
     1621 tgttgaaggg aacgaaagct tctgatgaaa ccgtaacttt gactttagat gaaaagaatc
     1681 aagttgccgt taaacactta gcgattaatg agtattactg gcaagaaacc aaagcgcctg
     1741 aaggatatac cttggatgaa acaaagtatc ctgtatccat caaaaaggtg gatgatagcg
     1801 aaaaaaatgc tgtgattact cgagatgttg cggcaaaaga acaagtgatt cgctttggct
     1861 ttgatttctt taaatttgct ggatcaacta ctggcactgc cgaaactggc ttcaatgatc
     1921 taacctttaa agtgtcgcca ttggaaggaa ccaatgaaat caccggtgcc gaagatgaag
     1981 cgaccacagc ttataacgag caattagggt ttgatggtta tggcaagttt gaaaatcttc
     2041 cttatgggga ttatttactt gaagaagtag aagccccaga aggttttcaa aagattacgc
     2101 cattagaaat ccgttctact tttaaagaaa ataaagagga ttataccaag agtgattata
     2161 tctttactat taccgaaaaa gggcaaaagc aaccgattaa aacggtaacg gtcccttatg
     2221 ggaatctaac caatgagaat ttctcagtca gtttgaaccg tttgatgctt tatgatttgc
     2281 ctgaggaaga agatagctta acttctcttg cgacttggaa agaaggagat aaagaattga
     2341 ctactcttga ttctaccgag ctagtcgata aattgagcta taatctacat gaaatcaaag
     2401 aagactggta tatcgtagct caagccatcg atgtagatgc tacaaaagct gcccaagaaa
     2461 aagataaaaa agccaaaccg gtggcgattg cggaaacaac cgcaacttta gcgaataaag
     2521 agaaaacagg aacttgggag attctgcata aattaactgt cgaacaagtt ttagacaaga
     2581 ccattgtctt attcaactat gtgtatgaaa gcaaagaagc ctatgaagct ggtgatgagc
     2641 cggtagcgaa ggatgctagc ttgaacaatc aagcgcaaac tgttaattgt atggtggaac
     2701 gtcatgtttc catccaaacc aaagcgcact tagaagaagg ttctcagacc tttactcatg
     2761 gtgacgtggt agatatgttt gatgatgtat ccattaccca tgatgtacta gatggctcaa
     2821 aagaagcttt tgaaacaatt ctgtacgctt tatttccaga tggtacaagt aaagaaattt
     2881 ggaaatctgg caagattgat tacgaagtga atgacaaaga atttaccaaa accgtacttg
     2941 cggaaaaagt ggatacaagt aaatatccag aaggtacaag ttttaccttc aaagaaatta
     3001 actatgacaa agatgggaat atcaatggaa aacacaatga agatttgaaa gaaaaatctc
     3061 aaactttaac gcccaaaaaa gttccgacaa caccaagtac accagaacaa tctgaaatgc
     3121 caactgttcc aagtggctct caagaatcta gtcctacagt gaaggcattc cctcaaaccg
     3181 gggagaaaac ttccaatgtg ctactgttca ttggctttag cttgatcttt gcgacggctg
     3241 gctattattt ttggaatcgt cggaactaag gtgataagat gaaacatgta aatagtagaa
     3301 aacaatacgc gcgaccgcct cctaaaaaag tggtcgcgct atttttgtat tcaaaactaa
     3361 caatgaaaag aggaaaaaat atggaattga aatttgttgt accagatatg gtcgagacgt
     3421 tcggaaaact aacatacggt ggtgaaggtg aagttctatc ggaagggtat ggtcgaaata
     3481 ctacaattgt tggtcgaagt taccatctgt tttcaaataa acaacgggcc gatgatattg
     3541 aagtggtggt agctgcggaa gccggcgaaa aggattttga ccaagatcaa ccactaaaag
     3601 ctgtaaatcc ccacttaatt gccaaaggct atgaaattga gaatcgtggg tttaccgatt
     3661 acgtgttgta tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag
     3721 aaggtattgt cgtagattca ggattaacct ttggcaagtt gcgtttttcc gcattacgtc
     3781 gagaagtacg caagcaaaat gaggacggaa cggttagtaa cgaggtaaaa gaacggacct
     3841 ataatttgaa atcctccgcg caaggtcgaa tgatccaagt cagtattcca gcaaatgtac
     3901 ccttacgcga gtttgcttat gatgcagagg tagagctggt caatccaatc gtggatacgg
     3961 tcgccaatta tgtttttcgg gaaggaacca ccgttaattg gtttatcaag gcagatgatt
     4021 tagtcttgaa acgacaactg aatcaaggaa aacctacgaa ccaaaatgaa ggaaagaaat
     4081 aggtgaagaa tatggaaatt aatgttgaac atattttaga ttgtctcgat caatttggca
     4141 aaggtgagct aacggaggaa caactcacta acgcattaac ctatgatgaa aaaatgtttc
     4201 tgattatgca tcaagggtta ctggaagtag gtaacgatgc caaagaagac tttgatgtac
     4261 tggattggtt aggggaagaa gagtctttct ttatggttgt cgaggtaaat gaaagtttat
     4321 gtcggcaggc agaatctgtt ttggaagaaa tcgatgtgga gatgccggat gccatcgaaa
     4381 gatttttaaa acaattagtg gaaacgaaac aacttcccgt agtagtaaat gactagcata
     4441 actttttgaa attgcccaat gaaatcctat tgggcttttt cgactggagg tggaaaccat
     4501 caaaatgtac aaaggacatc gcatacgcgc aggagatcaa cacttgattt atcactttgt
     4561 tcttggctgg ctaatctttt tgttcattgg ttggatgagc gtcttttatt tacaagaact
     4621 tagacaattt gatatttcta aggtatcatt ttccactagt gaaattggct ggtctatgaa
     4681 ggatgtaatc tgcttgctag gaagtctcgc tctttcagga agcatgatgt tactttatat
     4741 acactttttt caggatcatt ggcgaagtct ttggcatcga caaaagttag ctcggatgat
     4801 tctagaaaac cattggtatg aagtaaagca gactcaaggt gaaggttttt tcaaagattt
     4861 gaatagtagt tggaccaaag agagtatcag ttacttcccc aaaatttatt atcgaatgaa
     4921 agatggctta ctttctattc gcgttcaaat ttcactggga aaatatcagg atcagctatt
     4981 gaagttggaa aataagttag aaagcggact gtattgtgag ttagtagaaa aagaactcaa
     5041 agactcctat gtggaataca ccttgttata cgatatgatt gccaatcgaa ttgggataga
     5101 agaaatagtg gcagaaaatg gggcgttgcg attgatgaaa aatcaagtat gggcgtatga
     5161 ttccttgccg catatgttaa ttgctggcgg cacaggtggt gggaagacct atttcttact
     5221 caccattatt caagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa
     5281 tgcggacctc gccgatttag gtacgataat gcctcacgta tactcccaaa aagaagaaat
     5341 ttctgaatgt gtggaagatt tttacgaacg catgatagct cgtagtaggt caatgaaaga
     5401 aatgcccaac tacaaaacag gagagaatta tgcgtatctc ggacttccgc ccaacttttt
     5461 aatctttgat gaatacgtgg cttatatggg tgcgaatcgt tttcctacaa gcatcgaata
     5521 gatgtgaaat ggaagcctat tgagattatc aatagtgaca actaatgttt tgagagatgg
     5581 aatgaaagcc ttcaagttga gctgaaacat ccccgctaat actcctacat gctctaatca
     5641 atgaagtaga ttggttttgg tatgaagcta ggtgaagtcg tctgaaatct gcctaagtgc
     5701 ttgagcataa ggctgactat agggcgggcg gctgaaaaag ataactatgg taagaatgtc
     5761 tgaaaatgac tgacgaatct gcgaatgtac gggtctaaaa ggctgatctt attgaaagat
     5821 aggaacatac ggacgtatgg tgagataccg acaagtaagg ttccatgata tgaaaatcgg
     5881 gatagctaac gaagaagcta taatttcaca ggctcatagt agatacctaa ggttatacat
     5941 gatagctaaa aacattggaa cgatgtaagc agaaaactat caattaaatg actacttcta
     6001 gataggtgca tataagtaat aacgaaattg ctttgtttct gtgaaagtaa gggcacagta
     6061 ccgatgaagc gtctaacaaa tgtggaggga tagccctaag tctatttaat aacaatgaat
     6121 gttgaaacag atgaagttca aggatctggt aagaacgtgg gaaaatccgt tatggaggtg
     6181 ccacgcttga acacaaaaat acgttactgg gaatattatg atctacaaga aacattcgat
     6241 cagctattta ctcaaagtag aaacgggaaa aagttttatc aattatatga gttaatcatt
     6301 tctgaaaata atattctttt agcataccgt actatcaagg caaacaaggg gtcttcgaca
     6361 ccaggaacgg attccttcac aattgataac tataaggaaa tgaaccaagc agagtttatc
     6421 catcttattc tcagtcattt agaaaattac aaaccaaaat ccataaagcg agtaatgata
     6481 cctaaaccca atggtgagaa acgcccatta ggaattccat gcatgattga tcgaatgatc
     6541 caacagatgt tcaagcagat actggagcca atatgtgaag ctaagtttta tgaacatagc
     6601 tacggtttca gaccattgag aagtgcaaag catgctctag gtcgcattat gtatctcata
     6661 aacatcagta aaatgcacta tgcagtagac attgatatca aaggtttctt tgataatgta
     6721 aaccaccgtt tactgataaa acaattatgg aatataggaa tttgtgacaa acaagtctta
     6781 gctattcttt caaaatctct gaaatcaccc attcaaggag aaggtatccc gagcaaagga
     6841 acgatccaag gaggtatcat ttctccacta ttatctaatg ttgttctaaa cgacttagac
     6901 cattgggtct caaagcagtg gcataccttt gaaacaaagt atccctatac taaaggttac
     6961 aataaatttc gtgctctaag agatacaaat ctaaagcaag gctatattgt caggtacgca
     7021 gatgacttta aaatcatgac taatgattat ccgacagcat taaaatggtt ccacgctgtc
     7081 aagctttacc taaaagacag acttaaatta gatatttcca atgagaagtc taaaatcgtc
     7141 aatttgcgta aatgtaagtc ggaattttta ggattcgcta tttgcgtgaa acaaaaaggg
     7201 aaaaaatggg tttgtaattc acgtatttct aacaaaaaaa aggatcagat taaagaagag
     7261 attaaacaaa gaatcaaaga tattcaaaag agtcctacag cacaaaatgc tctcctgttt
     7321 aatgctctcg tgctcggtgt tcataattat ttcaggtatg ccactcatat taatttagat
     7381 ttgaatagga tgaactacgt tctttcattc actacttaca accgattatc aaattgtagt
     7441 acaagatgtt accccaacaa agcttcccca acttacaaaa agttttactc aacaacgatg
     7501 aaaacgtata aagtagctgg agtctatttg taccctcttt gcgacgtatc tacaaaaaca
     7561 atttacggat ttaatacgga ggatacaccg tttactccgt ttggtcgtca aagattggag
     7621 cacaaaagcc tacagtctct tgtgttccaa gaattaagaa aattgatgga atcgaaaata
     7681 ccgaatagaa cggttgagta tttagatagt agaatatctc gttactcaat gaaatcaggc
     7741 aagtgtgaga ttacaaaaca atttttgcca gcaaaagccg ttcactgtca tcactatttg
     7801 ccaaaaagtc ttggtggaga cgacaaattc gataacttga gaattattca taaggatatc
     7861 catcttctga tccatacaac aaataaaatg ataatcgatc actacgtgca tgaactgaaa
     7921 ctggtgccag agcaaatagc aaaaatcaac ctttatcgaa agatgtgtaa tctgcaaaac
     7981 attcaataga aaataaatag atggaacgcc gtatgaggtg aaagtctcac gtacggtgtg
     8041 gagcggggga aaagatggag attatatcaa attcttacct atcgctatag atgttaacga
     8101 caaaagaaag tgcggtgatt ttgaataagc ttaaacaaat cgtgatgtta ggccgtcaat
     8161 ctggtttctt tctgattcta gcttgtcaaa ggccggatgc gaaatatcta ggagatggga
     8221 ttcgcgatca gtttaacttc cgtgtggctt tgggtcgtat gagtgagctt gggtattcca
     8281 tgatgtttgg agaagtcgat aaaaattttt ttatgaaacg catcaaagga cgaggttatg
     8341 tagatacagg gggcagtgtg attagtgagt tttatacgcc acttgtacca aaagggtatg
     8401 atttcttaag ggaaattagc aacattgttg gattaaccgt tcatacggaa agagaaaata
     8461 atagtgtgta gcctaggaat atcaataatc ctgggctttt ttattgaggt gatttatgac
     8521 atttttagat ttattcgccg ggattggtgg ctttcgctta ggtatggaac aagccggtca
     8581 ccagtgtatc ggtttttgtg aaattgatga atttgctcgg aggagttata aagcaatcca
     8641 tgatacaagg aaggaggtgg aaatgcatga catcacaagt gtatcagacg aatttgttcg
     8701 atccctcgga ccagtggaca tcctttgcgg cggctttccg tgccaagctt tttcaattgc
     8761 gggaaagcgg caaggatttt ccgatactcg aggtacttta ttctttgaaa tcgctcggtt
     8821 cgccactctt ctccaaccta agtttctatt ccttgagaac gtccggggat tgctcaatca
     8881 cgaaggaggg gctacgttcg agacgatcct ccgaacgttg gatggattgg ggtatgatgt
     8941 ggaatggcaa gtgcttaact caaaggccta cgttccccaa agccgtgagc ggatcttcct
     9001 tatcggacat tctcgagatg catgtaccga acaagtattt cctatcattg gatcgtcttc
     9061 aacatctgat caaaacatca gaaacttggt aaatatcaat ccttccaatc gaggaatggg
     9121 tggccaagtc tatggttcag atagcgtagc accaaccttg accggtgatg aaggaattaa
     9181 aattgcctta ccggtaaagg atgggatttc tgtagcgggt atgttgtcgg gaaattttga
     9241 acaaggcaat aggatttatg aggtaaccgg aacagccccc actttgtcaa cgaaacaagg
     9301 gggaacgaag attatgattc ggaaaaatga aacagaatat caagaggtga agccagggga
     9361 cagtgtgaac ctggcttttc ctaattccac aagtcgccga ggtcgtctgg gaaaacaaag
     9421 tgtgcatacc ttactaacag gagaccaaca agcggtagtc acggatcagt accagattcg
     9481 aaagttaaca cctagggagt gctggcggct tcagggcttt ccagattggg cttttgatcg
     9541 agcttctcaa gtcaattcag atagtcagtt gtataaacag gccggaaact cagtaacagt
     9601 tccggtcatt tttgatattg ctagaagatt aaagggggta aaagcatatg atttatgacg
     9661 agttgattgg agaaatctac tggatgattg gcaagattca ggcagatccg aaattggaag
     9721 aagaactgta ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc caggtgattc
     9781 gtatgtagtg gatgaggaaa cggatgctcg aatggagctc aatcaagtca tcggtgaatt
     9841 ggaacgaatt gcggaccttg ccaaagaacc tgatatccgc caatacttgt ttgagataaa
     9901 agcagagttg gaaattgagg gtatcaccgc ggagtaatag ggaaataacc cactgatagg
     9961 cactgaccgc gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc tgttcatgga
    10021 cggcggcagc cggccatgat gtctcacccc ccgtatctaa caggggggta caaaaacaca
    10081 aaaaacaagc aagtaaccat aaaaaatgct tgttgtatca aggatattaa gtaatttgta
    10141 taggtgttaa ctcatttgga aaagttaaca ccttgaattt ggaggtgaaa acttggcgca
    10201 acgaaattta gattatcggt tattaaaaga acgacgcaac gaatatggcg tttcacaaaa
    10261 taaattagct actgcttgcg gacttagccg cccataccta aatcagatag aaaacggtgg
    10321 cgtaatcgcc tctacaaaga ccatgagaaa aatttttgat caactggaaa gtttcaatcc
    10381 cgatttacct ttaacgttac tatttgacta tgtaaggatt cgctttccca caacggatgt
    10441 gcggaaaatt attcaagaaa ttctccattt gaaattcgag tatatgcttc atgaagatta
    10501 cgccttttac tcctatcaag aacaatatgt catgggcgat attgtagtga tgttatctca
    10561 tgaagaagat aaaggtgtcc ttttggaatt aaaaggtcgt ggttgccggc agtttgaaac
    10621 ttttttactc gcccaaaagc gtagctggta cgactttttt gaagattgtc taaaagccgg
    10681 tggcgtgatg aaacgcttgg atttagcaat caatgatcga gtggggctac tggatattcc
    10741 tgatttaacg aaaaaatgtc agaaggaaga atgtatctcc ttgtttcgta ccttcaaaag
    10801 ttaccgttcc ggggaactgt tgaaagctga tgaaaaggat ggtatgggca ataccttata
    10861 tattggcagc ctcaaaagcg aggtttattt ttgtttgtac gaaaaagatt atgaacaata
    10921 tatcaagtta gggattccat tggaccagac cgaaacgaaa aatcgttttg agattcggtt
    10981 gaaaaatgac cgtgcttatt atgcgattca agatttgtta aaaggtcgta gcattgaaag
    11041 taccacgttt tccattatca atcggtattt gcgatttgcg gataaagtag atggaaaacg
    11101 gcgaaccaat tggcccttga atgaacaatg gggtcgtttt attggacgga atcggaaaga
    11161 gattcaatta acttctgaac cgaaaccgta taccattgaa cgaacattaa attggctagg
    11221 tagacaagtc gcccctacgt ggaaaatggc taaggagttg gatcgattga atcaaacaac
    11281 gtatatccag gatatggtac agaatgcgcg actctcggac cggcataaaa agattttgga
    11341 gcaacaaagt atggcaatcg aaaacttgat tgtatgaaaa gaggaatttt gatggaaatt
    11401 gtaaaagata tgttattggt acttggtggc agtatgcttg gtgttaccgt gatgtgcttg
    11461 atgaatgcaa gtgcagcagc ggattgagca atggaaaaga aaggaaagtg acaaaatgaa
    11521 tttcggacaa aattt
//
LOCUS       DAZUMI010000099        11128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000099 DAZUMI010000000
VERSION     DAZUMI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.100"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="ACG81C_002217"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="ACG81C_002217"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5065360.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="ACG81C_002218"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="ACG81C_002218"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5065361.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /gene="dapA"
                     /locus_tag="ACG81C_002219"
     CDS             2139..3020
                     /gene="dapA"
                     /locus_tag="ACG81C_002219"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5065362.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /gene="dapB"
                     /locus_tag="ACG81C_002220"
     CDS             3017..3733
                     /gene="dapB"
                     /locus_tag="ACG81C_002220"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5065363.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="ACG81C_002221"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="ACG81C_002221"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5065364.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="ACG81C_002222"
     CDS             4450..5451
                     /locus_tag="ACG81C_002222"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5065365.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="ACG81C_002223"
     CDS             5448..6809
                     /locus_tag="ACG81C_002223"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5065366.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="ACG81C_002224"
     CDS             6769..7920
                     /locus_tag="ACG81C_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5065367.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="ACG81C_002225"
     CDS             7895..9064
                     /locus_tag="ACG81C_002225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5065368.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="ACG81C_002226"
     CDS             9239..10618
                     /locus_tag="ACG81C_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5065369.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="ACG81C_002227"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="ACG81C_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2494 g         2987 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAZUMI010000100        11066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000100 DAZUMI010000000
VERSION     DAZUMI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.101"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..1702)
                     /locus_tag="ACG81C_002228"
     CDS             complement(122..1702)
                     /locus_tag="ACG81C_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5065370.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2103..3479)
                     /locus_tag="ACG81C_002229"
     CDS             complement(2103..3479)
                     /locus_tag="ACG81C_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5065371.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3607..4695)
                     /locus_tag="ACG81C_002230"
     CDS             complement(3607..4695)
                     /locus_tag="ACG81C_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5065372.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4867..5301
                     /locus_tag="ACG81C_002231"
     CDS             4867..5301
                     /locus_tag="ACG81C_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5065373.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5379..5912)
                     /locus_tag="ACG81C_002232"
     CDS             complement(5379..5912)
                     /locus_tag="ACG81C_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5065374.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6006..7184)
                     /gene="mutY"
                     /locus_tag="ACG81C_002233"
     CDS             complement(6006..7184)
                     /gene="mutY"
                     /locus_tag="ACG81C_002233"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5065375.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7177..7974)
                     /gene="recX"
                     /locus_tag="ACG81C_002234"
     CDS             complement(7177..7974)
                     /gene="recX"
                     /locus_tag="ACG81C_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5065376.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8081..9463
                     /gene="rlmD"
                     /locus_tag="ACG81C_002235"
     CDS             8081..9463
                     /gene="rlmD"
                     /locus_tag="ACG81C_002235"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5065377.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9637..10215)
                     /locus_tag="ACG81C_002236"
     CDS             complement(9637..10215)
                     /locus_tag="ACG81C_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5065378.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10537..11007
                     /locus_tag="ACG81C_002237"
     CDS             10537..11007
                     /locus_tag="ACG81C_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5065379.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3298 a         2222 c         1909 g         3637 t
ORIGIN      
        1 tttaatatag taaaaagagc cgtcacggaa aatccgtcac ggctctaaac aaaatatgag
       61 aggctgtttt tgtttggcta ttcttattac ttgatgcagg gcagcttgtg tcaagacttc
      121 tttacatcaa actttttaat tttacattgg gatatttatc cgcaaaccag cgttctgcaa
      181 attggttctc gaataagaac aacggttggt cgaaacggtc acgagccaag atattgcgac
      241 ttgaactcat cttctcatcc aaatcttctg gatcgatcca gcgagcgatt ttgtgtccca
      301 ttggagacat gattacttcg gcgttatact catttaacat acgatattgg aatacttcga
      361 attgtagctg accaacagct ccaatgatat attcttccgt tagatatgtt ttataaagct
      421 gaatcgctcc ttcttgaact aattgataga ttcctttgtg gaaggatttt tgtttcatga
      481 cattttttgc agtcactttc ataaacagct ctggagtaaa tgaagggagt tcttcataag
      541 caacttttag ttttccttcg aacaatgtat ccccaatctg ataattgcct gtatcatata
      601 caccgatgat atcgcctgct actgcttttt cgacgttttc tctcgaatct gccataaatt
      661 gtgtgacatt gcttaatttc agttttttat ttgtacgttc caaccagaca tccattcctc
      721 gttcgaatgt tccagaacag attcggacaa aagcgattcg gtcacgatgg gctggattca
      781 tatttgcttg gatcttgaat acgaaacctg aaaattcttc ctcataagga cttaccatct
      841 gatcatcttg tgttttgtgt ccataaggag ctggtgccaa ttcaacgaaa gtctctaaaa
      901 aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa tactggtgtc tgatcgccac
      961 gagcaatttt ttcttgatta aatgcatctc cagcctcttt gattagttct acttcttcta
     1021 agacttgttc atataaggaa ttttcagtta gaggatgatt ttcgggaagc agtccatctt
     1081 catccaattc tactaatcgt tcatcctgat aattttccgg acgataaaac tctacacgat
     1141 tatggtagat atcataaaga ccttctagcc ctttacccat accgattggc caattcatcg
     1201 gataagattc gatatctaat agctcttcta attcttctaa caattccaat ggttcacgac
     1261 catcacgatc tagtttgttg ataaaagtaa atatcgggat gccacgtttt tttacaactt
     1321 ggaataattt ctttgtctgt gcttcgatcc cttttgcact atcgattacc atgaccgcac
     1381 tatccacggc catcaaagta cgataagtat cttcagaaaa atcttcatgt cctggtgtat
     1441 ccaagatatt tactcgtttc ccttgataat caaactgcat aacagaacta gtaacagaaa
     1501 tcccacgttg tttctcgatt tccatccagt ctgatttggc aaaattacct gtttttttgc
     1561 cttttacggt cccggcttga cggatagccc caccaaacaa tagtaattgt tctgtaatcg
     1621 ttgttttccc agcatccgga tgggaaatga tcgcaaaagt tctgcgattg tccacttgtt
     1681 gctttaattt tggattattc ataacctaac tccatttctt ccattcaatt atttatattt
     1741 tctctaggca cagattgtcc gatctcttaa agaaagacaa tttcatatag ggctgtgaca
     1801 aaagccgtgt agcagtctct ttttccgagt aaacggcgtt ctagagacag cctatcagca
     1861 ataagcccac ccaagccaaa aatacgaaaa gctgtttttg cttggctgct cttattactt
     1921 aatgcaggat agctttttca caacctttgt caactactac aactgaatca gtatagcatg
     1981 gattcagcga atgaaaaagc caccaaacca taagagtcaa atattttttt gtcagctgac
     2041 taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg tctttcataa ccaagtttcc
     2101 aattatcttc ttggctcgtc atcttcgaat tcttttctga agtatcttcg ttcttcttca
     2161 ggttccgctt ctgtttcttc cggatcatga aaaatgacac ggattttaag gattcgagaa
     2221 ccttccattt cttcagaaac cagcgtaata ttatcgacat caaacgataa cttttcgcct
     2281 tcatccggaa tcatgcctaa tgctgtaatc aaataaccag ccatcgtatc tacatcactc
     2341 atatgcagat cactgtcaaa agcttcattg aactcatcaa tcagcatacg tccttggatg
     2401 atatattcgt gttcatcgat tttttcataa agattctcaa cttcatctga ttcatcatcg
     2461 atttctccga cgatttcttc taaaaggtct tctagtgttg ctaacccaac gacgccgcca
     2521 tattcatcta ataaaatagc catctgattc tgcgtctttt tcatttcata taacagatca
     2581 tcaataaaga ccgtttcagg aacgaataaa ggctcctgca tgattttctt gatatctaaa
     2641 ttatcaaagc cgaatttatg ggcagctttt agtaaattct ttgtatgaag gattccgact
     2701 actttatctt tgtcttcgtt ataaacaggg attcttgaat agttttcgga taaaacttca
     2761 ttcacatttt cttccacagt gtcgttgata tcaatcataa atgcatccgt tcgtgggacc
     2821 atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc cttggagcat ttctaattct
     2881 tcattattca atacgccttc gttttcaagc atataacgca tttcatctct ggtcattttt
     2941 gaatcttcat catcaaaagt catcggcgtg atacgcgcca ataaacttgt ggaagcagaa
     3001 agcagccaga cgaagggctt agctactaca ccaatcaagc gtatcagccc tgaagtgaac
     3061 tttgcgactt cttctgattt atttaaagca atacgtttag ggtacaattc tccaaaaaca
     3121 atcgatacat aggtcaaaat agccagcaca atgatatttg caatattttt tgccgctgtg
     3181 ccgccaccta ataccggagc taaccgttcc gataaagtat tagctagtga agcaccagac
     3241 aaaatgttga ccaaagtgat tcctacttgg attgtcgata ggaaattatt tggattctgc
     3301 aatattttta aaagcttttg agatttcaca tctccttcat cagctttttg ctcgattcga
     3361 ttcttattga tcgaaacaac agcaatttcg gaagctgcta aaaaagcatt gatcaatgtc
     3421 aaaacgatca ataataaaat ctgcgctaac agcgactgac tctcagggtc agcattcata
     3481 atcgcattct ctccttataa aattaatttt tagtttacga tagaaaaaaa cgtggcacta
     3541 tagccacgtt aaaatggtat cacaaagcaa ttttctttgc aataaaaaca agtgtactaa
     3601 aactacctac gaggtcgtag caattttctt cttgttttca ttttgcttgt cttctttgac
     3661 gattttgatt atataggaaa tgatcgtctt gcagattgcg taaaaaggaa cacctaagaa
     3721 gataccaagc aatccggcaa tatttccagc tactagaaga acgataatga tcgttaatgg
     3781 atggattttc agtgatttcc caattacatt aggataaatg atattcccat ccaactgttg
     3841 gacgattagc acaaccacac aacaaagtaa tgcttttacc ggatcattga agactgttac
     3901 tagaaatgca ggtgctagtc ctagataagg tcctaaatat ggaattaggt ttgtcaatcc
     3961 cgcaatgacc ccaaataaga aagcataatc cacaccaatt accatatatc ctaaaaatgt
     4021 gaaggtacca acgaataaac attcgattgc ttgtccgcta atgtaattag ccaatgtttt
     4081 attcagttgc cccaataact ccacaatttg gtttcttctt ttttctggga aaaactgttg
     4141 gatgtttggc actagttttt ctccgtcttt caacatatag aacaagatga atggtgctgt
     4201 aatgattact acggttgccg aagcgatcgt tccaacgatc gaacctaaac tattggatac
     4261 tccactaagg aattgctgaa tgattgtccc gtaagaaatg tctaattgct caaaatattt
     4321 attgatatct acttccttga acaaaggcaa ttcggctacc tcttttaacc agctttctac
     4381 ctgtttgatg aaatcaggca tattagatgc caatgaggaa atttgctgta ctagacttgg
     4441 gatgacacta agtaaaatcc aaataattgc tgcaactaaa aggagcagca caattgcgac
     4501 agcccatatt cttttgattt ttgctttcat caacagcttg acaataggat tcaataaata
     4561 gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg gaaaaaaatg tcccgatcgg
     4621 ttcgaaaata aagtttattt ctgatgaaat catgatcaat gcagctatga tcaataattc
     4681 aagcgaccaa aacattagtt tagactgtct aagtttttca aacaatagat ttcctccttt
     4741 ctttcttact agtatatatt atattcgtta aaaattcttt tattcttttt ttatttttga
     4801 aaacttgcca tactttttat ttgttttatt ttccagtata ctatacgaaa ggaaggtggc
     4861 tagaccatga aagttgtaca aacaaaagat acaatgagca atatttactt agatgctgta
     4921 cggatcaggc atcaagtttt tgtagtcgaa cagggtgtgc ctttgtctcg agaaatagac
     4981 aaagatgaag cccattgtat ccacttcgtc ctgtactcag ataaaaaaga accgcaagga
     5041 actgtacgtc ttttgccttt agaaaacgga aaaatgaaat tacagcggat ggcgattctt
     5101 tctgaataca gacaccaagg gttaggaaaa atattgattg aagaagcaga aaactttgct
     5161 aagaatcaag gctataatac gattttatta ggggcacagt ccacagctga aactttttac
     5221 gaaaaactag gatatacagc ttacggcgat ccttttgaag atgctggcat gcctcatatt
     5281 tatatgaaaa agactttata aaaatttatt tcgaaaaaag caaaaaacag caagcttttg
     5341 agaaaaatct ctcactggct tactgttctt ttttgtgttt acttacgcga caattgcttg
     5401 taacggttat accaaatatc aatatagcct tccgagaacg ggccatctcc attattgatc
     5461 cagtcaacca gaatcttcac attttctttt aggatgaagt caatatcgtt cgggtaattc
     5521 atcattttgc tgtgcatctc atattcatcg acatccaata gtcgtttctc gccgtcagga
     5581 aataccttga tatccaagtc atagtcgata tatttcaagg cctcatcgtc cagcaaaaaa
     5641 ggtgaagcta gattacaata ataggaaacc cccttttctc tgatcattgc tattacattg
     5701 aaccagtatt ttttatgaaa ataaacaatc gctggttcgc gcgttaccca tcggcgtcca
     5761 tcagattcag tgactaatgt gtgatcattc acgccgatca tagagtgttc gcttgttttt
     5821 aataccatgg tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta actttggatc
     5881 gtcacaaact ctccttcttt tggaactccc atagcaaccc ttccttaagc agcattttgt
     5941 gctttgtgtt tgggtgttca ttacaccgac atcataatta ttatatcaat aaaaatgagc
     6001 tttgcctaga aaggattcct attgtttcga tttttctcta actgatctac cagtttcatt
     6061 tggaccttag gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt cgaagttttg
     6121 ttctcggtaa attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag gagaacatgc
     6181 catttcagat gactaaaaat atgagtaact tctcctaaat gtcgtgtctg ccaaacaaag
     6241 agttcctgct tttcaaatgg gagattttgc tcttcttctg ctactaggtc atcaaacaga
     6301 tcgatttctg gctttgtttc ccattctttt ttcaatcgct catactcctc ttgtgtcact
     6361 tccatcattg gaaatgtcca catattcgcc agcaattttt ggctatctct ttcttcaaaa
     6421 taatacgctc cagaatgatt ttgaagagct gcactgatat aataaacgtc ttttggcttt
     6481 gctttttttg tttttacagg aaaactcgtt tggattcctc gtttgttagc taaacaaaag
     6541 gcttggatcg gacaagtctc acactttgga gaagttggtg tacagatagc tgaacctaga
     6601 tccatcattg cttggttgaa ttcacctgga tgcttttcat caatgatttt ccgcatcgct
     6661 tcatcaaaga ttttcctgct agaagctttt gcgatgtctg cttcaatgca aaacaatctg
     6721 ctgactactc gcatcacgtt gccgtcaact gcaggttctg gaagtccaaa tgcgatactt
     6781 gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg agctaatttc ttcaggcgtt
     6841 tgcggcattt ccccatcaaa ttctgacatg atctgtttag cagcagcttg gatgttccgg
     6901 gctcttgagt aataaccaag gccctcccat gcttttaaaa gcttttcttc tggagcattt
     6961 gctagctctt caattgtagg aaaccattcc atgaaacgat aaaaataatc gatcactgtg
     7021 tctactctgg tttgctgaag catgatctca gagatccaga tacgataagg atcacgatta
     7081 tagcgccaag gcaggtttct tttttcttgt tcataccatt gaatgaactg atcttgaaat
     7141 tcttttgttt ctttatctgt ccatttttcc cattctttat tcactttcct cttccttttc
     7201 cgtaatgaat cgctggatca tatccaaatc aaatcctttt tgatataagc tttgctttac
     7261 ttttagattt ctttttttag gttcaaacct taaatttctt cgccatagtt tatctccttg
     7321 tttgactaaa taatcatatt ctatttcttg atctgcttct tgcggaagat cagctatcgc
     7381 ttgttggatc acttcttgag aaaagccttt cgtcatcaat gtttgttgga tttttctcaa
     7441 taattctttt tggctttttc cgtgattttt tccaaacgct ttttcggctg tctgatgtgc
     7501 attttgaact tggtcttcaa atgtatattg gctgagcgct tgttcaatga tttcaggttt
     7561 caaacctttt tgctgcatct gctgcgctaa tttctttggt cctttgtcac ttaagcgcat
     7621 attcgtgcgc acataacttt ccccatagac caaatcatcg acaaggtcca attcttttaa
     7681 gcgggctacg attttatttc gatcttcttg aaggatttcc ttttctttta agtatgttcg
     7741 gatttctttt tccgatcgca actgataact taagtaattc attgctaggt gaaaaccgaa
     7801 atcagctccg ctactcttct tgatctcttg gatagtttct tcgtctaatt cccgtccttt
     7861 taataatcga tagcgtacta atacatcttc agatacttgt aaagcttctc cttcagataa
     7921 atctacgcga tagaaagggc ctttgccttt actgattctc gtaacggtta acataaacat
     7981 cctcctcgaa catatattct ctatcatacc acagaaaatc cccgtaatca ggtattatat
     8041 gttagaataa accacagaaa aaagaaaaag ggagatactt atggcagaaa caattgtcaa
     8101 aactggacaa acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg aaggaatcgg
     8161 ttatttcaaa cggttgatca tatttgttcc atatgcacta ccaaaagaag aagtactagt
     8221 gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa ttaataaaag tcaaaaaaac
     8281 aagcaaagat cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg gctgtcaatt
     8341 acaacactta gcttatcaag ctcaattgga ttttaaaaag gatctgcttt tgcaagcatt
     8401 ggaaaaattc aaaccagcag gctttcgatc ttatcaactt ttgccgacga tcgggatgga
     8461 tgagccttgg cattatagaa acaaagcaca atttcagtta cgaaaaaata agcagactca
     8521 aaaaattgaa gctggtttat acgaagcaaa ctctcatcag ttagtcccta taacagactg
     8581 cttggttcaa gaacctcgaa cacaaaaagt aatgaatact gttgttcaac tattgaataa
     8641 atttgatgca cccatctatg atgaacggac aaacagtgga atcttccgca caatcatggt
     8701 tagaatcggt gttaaaactg gggagttaca agttgttttt attactcgca gtaaaaaatt
     8761 cccgcaaaaa aacgcaatga tccgtgagat caatgaacga ctgcccgaag ttgtttcaat
     8821 catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa cgatccactt
     8881 atggggaaaa gaagccattg aagaacaaat caatgaagtg actttcgatc tttctcctcg
     8941 tgccttcttt caactcaatc ctcagcagac cgaattgctt tatggcgaag gaatcaaagc
     9001 attagatgtc cagcctaatg aaacgatcgt ggatgcttat tgcggagttg ggacgatcgg
     9061 cttaagtgta gcaaaacaag ccaaagaagt acggggaatg gataccgttc ctcaagcaat
     9121 cgctgatgca aaaaaaaatg cggaaagatt aggatatcac aacactcgat atgaagtcgg
     9181 gacagcagag caagttctgc ctaaatggtt gaatgaaggt tttcagcctg atggtatcat
     9241 cgtagatcca ccacgtacgg gtttggataa cgcattgatc caatcattat tgaaataccc
     9301 tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca cttgccagag atctggttaa
     9361 tttaagtaaa aagttcaaga ttgaatattt acagtccgtc gacatgtttc ctcagactgc
     9421 acgttgtgaa gttgtcgtga agatgacgag actcgctgac taaatgaaag aggttgtgaa
     9481 aaagtctgcc ctgtatcaag taataagaac agccaaacaa aaatagctct tcatgtttta
     9541 tgaatactgt acttgtgacc gaagagttaa caggaggaca ctatttattt aaaaaaaggg
     9601 ttgtgacata gtttttgtca cagccctttt tgattattat tggatattca cgtctatcac
     9661 ttgataaaat gcatttgtcg tatcgctgat attccatact gctaaaatca cattatatcc
     9721 ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa cagttgcttt
     9781 gtcgtctatt tttgtgatca attcaaaatc actaaatttc aaaggttgat taggattcca
     9841 agttggtttg gtaatgtagt aatcccaagt acttgtccga tgttgtgctg ttaaattcca
     9901 agtaatattt aatgttcctg gattaaccat gtttttatgc catctgttag atgtctgttc
     9961 atctaaaggc tcaaagccgc tgactcctgc actagcaatt tttccatcaa tgaatgtatt
    10021 tttggtagct tcgatacttt gcggttccca ttgtgctctt ccgacatttt gatttaagtt
    10081 tcctgctgct gatgttccta ggtatgcacg gcttcctgga gaagtaatat aaccatgcgc
    10141 aaaacctgga atgctaaaac cgaacaaccc taaactaact aacccaataa acaataacgt
    10201 actaatcttt ttcatgtaga aacctccttc atttgataac cacattgtaa acgctttatt
    10261 gtattaacac aaacattatg cttttataat tttatgttga agtaaaaaag gtgaaaaaag
    10321 taagatgcag attataacag aagtagacga gaaaagtaca aatagaaaac atgagaatca
    10381 aatcatatga tctttttcaa aaacatcatt catataaaca aatgttcaac tttttaataa
    10441 caacaattat caattttttt cttacttcac aaactaaata caggtcttgc tgtgagcctt
    10501 catggtaaac taatattata gaaaggagtt agtaaaatga agatgataaa agatattcgt
    10561 ttttgggttt gtgttatagg aattgtcata ttaggctttt tatcagggtt gcttagcgga
    10621 aatcccggaa aatattacta ttctttgcaa cttccaccat ttgcaccacc tagctggata
    10681 tttgggccga tgtggacctt actgtatata ttgatgggta tttctcttta tttgttactt
    10741 aatcacaaca acaaaaaaca gcgaaacaat cttgtcggtc tttttgtgat tcagttcatt
    10801 tttaatttta tatggtctgc tctatttttt aatttaagaa atattttcat tgcagcaatc
    10861 gatattacac ttttagttat tttcttgtca gtgcttatgt atcagttgtg gctgcatcat
    10921 cgcctggcaa tgtggctcat gattccttat tatttatggg tactattcgc aactctattg
    10981 aattattcaa tttatttttt aaactaagcg ctaaaaagag acgtcctgta atttaacaca
    11041 tagaaaaagt gccacctaag ttagat
//
LOCUS       DAZUMI010000101        10962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000101 DAZUMI010000000
VERSION     DAZUMI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.102"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..680)
                     /locus_tag="ACG81C_002238"
     CDS             complement(18..680)
                     /locus_tag="ACG81C_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065380.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(693..2195)
                     /locus_tag="ACG81C_002239"
     CDS             complement(693..2195)
                     /locus_tag="ACG81C_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065381.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2743..4422
                     /locus_tag="ACG81C_002240"
     CDS             2743..4422
                     /locus_tag="ACG81C_002240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5065382.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4549..5028
                     /locus_tag="ACG81C_002241"
     CDS             4549..5028
                     /locus_tag="ACG81C_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5065383.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5247..5927
                     /locus_tag="ACG81C_002242"
     CDS             5247..5927
                     /locus_tag="ACG81C_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5065384.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5935..7266
                     /locus_tag="ACG81C_002243"
     CDS             5935..7266
                     /locus_tag="ACG81C_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5065385.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7281..8243
                     /locus_tag="ACG81C_002244"
     CDS             7281..8243
                     /locus_tag="ACG81C_002244"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5065386.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8354..8692
                     /locus_tag="ACG81C_002245"
     CDS             8354..8692
                     /locus_tag="ACG81C_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5065387.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8771..9478
                     /locus_tag="ACG81C_002246"
     CDS             8771..9478
                     /locus_tag="ACG81C_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5065388.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9581..>10962)
                     /locus_tag="ACG81C_002247"
     CDS             complement(9581..>10962)
                     /locus_tag="ACG81C_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065389.1"
                     /translation="KRTIKELIRKINVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKL
                     AVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSL
                     IKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQ
                     IEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVS
                     RPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQ
                     FPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLRE
                     KIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQL
                     FRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKK
                     GYENKTKRM"
BASE COUNT         3483 a         1916 c         2305 g         3258 t
ORIGIN      
        1 gatcattatc ctgtttttta aacttcgatt ttttgaaatt ttttattttt tagttcaaag
       61 acttcatcac agctttttgc tacttcaatc tcatgagtca cgatgataat acagcgattt
      121 tgttcatggg cgatttcttg gaaaagtttg acgatatctt gtgaagttgt ttcatctaga
      181 tttccagtag gctcatccgc tacgattagt tgatgttcac agcagatagc acgaacgata
      241 gcgactcgct gctgctgacc tcccgaaagt ttggtcacct gtttttttgc taatatttca
      301 tcaattccta cacgagccag tcgatctaaa gcaacttgtc gataattctc ttgcttggtc
      361 ttagtgattt ccattgctgc tatgacgttg tctagagccg ataaataagg aagcaagttg
      421 taagcttgga aaataatcga gacatcattt ttacgataac ttcttaaccc gattttgttc
      481 agtgaacgat cttgatagta tacctttcct tcttttggtg catctagtcc agaaataagc
      541 gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa tagcgtacat ttttccagct
      601 tcgaaattca gttctacatc tttataaagc gcatcttgcg gatcttgata ccaaaatccc
      661 aattgttctg tccgtaacat caaattcctc ctttagttta ataggatttt tcttgggttt
      721 aatcttaata tccctataga agctaaaaag acagaaaaca gactgattcc aaatgctact
      781 ccggctaata gtcccaattg tgcaggtttg acattgattt ccaattcgct gacttgatca
      841 gaaacagcaa atggattatt cgtggttcct tgttgaccat cgcgttgacc gccttgttct
      901 tgtctgttac ctggcatttg tccaccgcca ggagcttgct ggttttgtgt cgtggtttct
      961 gtctgttggt caagtagctg ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa
     1021 gcaactccta atgcaagaat catacaaata gccacttcgg taaacaattg gagaatgatt
     1081 ttacttctgg attctcctaa agatagcaag acgcctaact catgccgacg ctcccgaatg
     1141 gtaatcataa tgatcagagt aagtatgata ataccggcta cagccactag taaaacaaca
     1201 tttttagaaa aacttgccac gttgtttaaa ggttccaaca ttgactgata catggaatca
     1261 tttgattgta agctaaatgt atctgtgtcg atcagcttct cagcttcttt gagaaactca
     1321 tccattttat ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat
     1381 gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg
     1441 aagttgaatt tcatacccat actgccgctt gtttcgctgg attcataaat tcctttgatc
     1501 gtcatttcat aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt atctccgact
     1561 gccaggtcat ttgtttctgc tagagtagaa tcaatgacta cattgtttgt gcctttgtcg
     1621 ctgtcatcta ttccttcacc atccgtgatt tttgctgttc catcagaaaa attactattt
     1681 tgtgcagttg ctgacacacc actgatttga aagtctgctt gagtcatttg cggattcccg
     1741 cccattcctc cagcaggttc accgcctgca tcagtagttt ctgaactgtc ttcacttgaa
     1801 tcagaactag tgatggcagt tattccatct tttgccatag ctgaggcgga actttcaaag
     1861 gaataactct ttacattatc taattcagct attttttccg cgtccgttaa ggaaactggt
     1921 gtaagagaaa aactgcctgg atctggacgt tcttcagtgg tagaaccgct tgaagaagtt
     1981 tgttcctgtt ttttaaatga agcttctcga ttggcactta aagtcactgt tgctccaact
     2041 tctttttgtg cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag gccagctagc
     2101 acaaaaataa gaatggctgt gaacacggca aacaataaca atgaacgtcc ccattttact
     2161 tttgtacttt tccatgcacg tttcataaaa ttcatatttt gcatccctcc tagtagatgc
     2221 atttgataat agacaaccta tcttaaagct agcttaaatc ttatgctgtt tgcacaccat
     2281 caacatgagg ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa
     2341 tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc
     2401 ctagatgcta cagagggatt tttacgtgca tagttgttct aacaattgct gatactagcg
     2461 atttagttta tttgttgtat aataatgggg aacagctgaa tacttatata agtattctgg
     2521 ctatagcgtt tcgcaaaaga tccattttgt taatgaagta tatccaaaat aaaatgccca
     2581 tccaactgtc agaacgcgga tgtgctggta attttcctgc tgattctcca taagttgaga
     2641 aatagtgatt ttgcatatgc ttcattggaa tggctcatat atttagataa agaagaaaat
     2701 cgacatcttt aaacttggat gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt
     2761 atcccattag gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag
     2821 gatattttta ttttagattg tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac
     2881 gtcgtaatcc ctgattttac gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt
     2941 ttaacacatg gacatgcaga tgcgatcggg gcacttcctt acttgttgtc aaaaatttct
     3001 gtaccagtat tcgggacaga gttgacgatc gagttagcaa aattgaatgt cagtcgtaat
     3061 gaagcagcca aaggatttaa ggatttccat gtggtagatg agcatacaga aatagacttt
     3121 ggtgaaacag tagtgagctt tttccgtacc actcatacga taccagattc agtggggatc
     3181 aatctgaaaa caaaagaagg tagtatcgtt tatacgggag attttaaatt tgaccaaacg
     3241 gctatcccaa tgtatcaaac agattatgca cgattggcgg aaatcggcaa agaaggagtc
     3301 ttagccttac taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc
     3361 caaatagcag atgaagtatt tgatacgatc aaatattggg aagggcgtat catcgtcgct
     3421 tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg atgcagcaga aaaagcacaa
     3481 cgaaaagttg tcttgactgg tcaagatttc gaacgaatta ttcgcacagc gatgcgttta
     3541 gaaaaacttc aattaccaag tgaagatctg cttgttacgt tgaaagacat gaaaaactat
     3601 gatccagaag aactgatcat tttagaaact ggccgaatgg gtgaacccat caagtctctg
     3661 caaaaaatgg caaacggtac acaccgtaat gtcaagattg aagaaggaga tttggtttat
     3721 atcactacta cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga agatatcatt
     3781 tatcgagctg gtggaacagt gaagttgatc tctgagaata tgcgcgtttc tggccatgcc
     3841 aatcctaatg acttgcaatt aatgatcaat ttgatgaaac caacatattt tgtgccagtt
     3901 caaggagagt atcgtgaatt agctgctcat gcagatttgg ctcatgcagt agggatgcca
     3961 tacaaaaata tctatattac tggacgcgga gacgtgctgg aatataagaa caagcgtatg
     4021 agtgttgcag gaactacaag tgcagaaaat gtaatgattg atggattagg cataggtgat
     4081 atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag aagacgggat tttcgtagcg
     4141 gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa aacctcaaat tacttcacgg
     4201 ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag aaagcagcaa tatgattgaa
     4261 acaatcgttg agaaacattt agaaagcaat gattttgagt ggagcaaatt aaagcaagac
     4321 atccgagatc aattaggccg ttacttattt gaacaaacaa aacgccgccc agtgatcttg
     4381 ccagtcatta tggaagctac tcaacgtcga aataaaaaat aaacaaaaag agtaagatga
     4441 acctctctat agagtgctcg tcttgctctt tttttgcgaa aaacaaataa tttttgaata
     4501 cagcatccct cgctttataa taaacgatag aataaaggga gtgaatcaat gatgagtcaa
     4561 acaaaagtcg aacgttattt agaagaacaa aagatcagct atcaaccact tgattttagt
     4621 catgtggtag aagaatcttt cacagaagaa ctacaaaaaa gagggattga tccagctttg
     4681 atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg tgatcatcgc attgattcct
     4741 ttgaacgatc acttagatta caaaaaaacg agacaagtga cgaaagatcg taaagttggt
     4801 tttcctggaa tggattttgt attaatgcat acaggatatc cccacggagc aaacacccct
     4861 attggaataa aactcgcaca tcctgattat tgttttttag cagacagttc tttaaaagaa
     4921 aaagaagaag taattgtgtc aagcggtgaa atcgggagaa gccttcggat aaaagtagcc
     4981 gatttagaaa agatcgtcca tcctatttac gctgatttaa ttagttaagg gacgagaaga
     5041 gaagctaagc agagcagaga cctcaaactt gagattatat ctgcttggta cttggtgcat
     5101 cctgtattat ctgttacaat tgctttgtac atactgtact atcaatccaa tgaaaatata
     5161 tttagcagct atattttttc attgatgtgc aagtgatgaa atcgttttta ataaaaaaga
     5221 tacagagaaa acgcgaggtg taaaatatga aaattacgta tcatggacat tcctgtttaa
     5281 caattgaaat ggaaaatggc caacgcgtga tgattgaccc gtttattaca ggaaatccat
     5341 tgacagactt aactgcagaa gaagtaaaga cagattggtt gctgattact catggtcata
     5401 gtgaccacat aggagatatg attcctattg caaaagaaaa tcaagccaca attatcagta
     5461 ttgttgagat tgctgattat gcgaacagca gaggagtaga ttcattcggt atgaatatcg
     5521 gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt acatgcacag catagttcaa
     5581 gctatgaagt agacggtatc gttcagtata tgggcgagcc tagcggaatt attattcaag
     5641 cagaaggaaa aacgatttat catgcagggg atactgctta tttcagtgat ctaggattgc
     5701 ttgcagaaga attcgatatt gatgtcgctt tcttgccgat tggcgataac tatacgatgg
     5761 ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc caagaaagta gttccaatcc
     5821 attacaatac tttccctgtc attgaacaag atcccgaatc ttttgtagag ttactggaag
     5881 atagtgaagg aaaaataatg agcgtctcgg aaacgctgga agtgtaggca gaaaatggcg
     5941 acaaaacatg atttgatttt agaatatatt gaaagtttac ctgttggcaa tcgaatttct
     6001 gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa cagcgtaccg tgcaatcaaa
     6061 gatgcagaaa atgtcggact agtttcaaca attcaacgag tagggaccat ccgtatcgaa
     6121 cgcaaattaa aaaaacatat tgaacgtttg acatttggcg aggtcgtcag gatcattgaa
     6181 ggggacgttt taggcggggc ggcaggatta gacaaggtct tgaataaatt cgtcatcggc
     6241 gcgatgacta aacatgccat ggaacgctat attacgccag gatcactaat gatcgtcggc
     6301 aaccgggcgg aagtccaaaa attagcattg gaagatggcg ccgcagtatt gatcacagga
     6361 ggattcaata cgactgctga gattgcaaag ctagctgatg atcttgaaat gccgatctta
     6421 cgcacctctt atgatacatt tacagtcgca accatgatca accgagcgtt aagcgaccaa
     6481 ttgatcaaaa aagatatcat gcttgtcagt gatatctata tgccagctga aaagactcat
     6541 tatttgcagc agacagatac gatttctgat tatcagcgtt tgtcagaaga aacgcagcat
     6601 tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg ggattgttac ggcaaaagat
     6661 gttttaggga aatcgccaaa ccagatcatc gatcgggtga tgacgaaaga accaatcagt
     6721 gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa tgatctggga tgggctagaa
     6781 gtcatgccgg tcgtttccga tgacttgaca ttggaaggct tgattacccg gcaagatgta
     6841 atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg cagatacgat ttcggatcag
     6901 atatctggaa atatccatac gattgatact gaccgtgaag gaaatcgttt ggaaaaccct
     6961 aagttcaaat tcgtagtagc accacaaatg gtcaatggcg ttggaaccat ttcttttggc
     7021 gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa tggtgatgaa ccaaaaacga
     7081 aatatcttga ttgaacaagt gaacctacat tatttgcggc tgatccaatt ggaaagcgaa
     7141 ttagacattc gtccacgtgt attggaaatc ggcagacgtt ccgctaaact ggatatcgaa
     7201 gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg tttgtcaagt aatggaacgt
     7261 tcttagaaag caggagtgaa atgacagtac agcaagaaat agtagataca ataaaaaaat
     7321 acgataaaat cattatccac aggcatttgc gccctgatcc tgatgcatta ggatcacaag
     7381 taggattagc cgaactatta cgggaaagtt tcccagaaaa agaaatacgt caagtaggtg
     7441 aaacgattga agggcttcgc tttttagcag aaatgcaaga agttgaagat gtcttctacc
     7501 aaggagcgct agtcatcgtg actgatacag ctaattcacc cagaatcagt gatcaaagat
     7561 atcagttggc agaaaaactg atcaaaatcg accatcatcc caatgatgac ccttatggag
     7621 atcttgtgtg ggtaaataca aaagcaagta gctgtagtga gatgattgca gaatttgctt
     7681 tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc acggttactt tatgcaggaa
     7741 tcgtcggaga tacagggcgc tttttgtatc cctccacgac gtcccgtaca ttagagattg
     7801 cttcgatatt gagaaattat ccatttgatg caagtgcttt gaacagagag attgaacaaa
     7861 tgccgatgaa agtcgctaaa ctttccggtt atctttatca aaatatccag gtggatgaaa
     7921 atggtgcagg aagaataatc cttccgcaag aaattcttga taaatatgga atcgatgatt
     7981 cagagacagc ttcgatcgtt tcacttcctg gagtgattga cgaagtgctg gcttggggga
     8041 tttttgttca gcagccttca gggtattacc gcgtacgttt acgttctaag gggccgatca
     8101 tcaatgaatt ggctaaacgc catcatggag gaggccatcc attagcaagt ggtgcgaatg
     8161 caaaagattt agcagaagta gaggaaatct ataaagagat ccaagatata tgccaatcgt
     8221 ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg tgccttttgt agtaaaaatc
     8281 acaaatgtat cttttcaaga tcttcttttt ccgttacaat agtgctcact aaagatttta
     8341 aaggagaaag aagatgacat taccaaattg cccagaatgt ggctcagagt acacatatga
     8401 agatcgaggc ttgttgattt gcccagaatg cgggaacgaa tggaacaaag aagaacaaga
     8461 aactgtcata gaagacggat tagtggtatt agatgcaaat ggaaaccgtc tgttagaggg
     8521 cgatagcgtg acagtgatta aagacttgaa agtaaaaggt gcgtcaggtg ccattaaaca
     8581 aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat cataatatcg attgcaaggt
     8641 agaaggattt ggaccgatga aactgaaatc ggaatttgtg aaaaagaatt aaagaatatt
     8701 caaggtcttt tcacattctt ttcctactac taatgataaa ataaagtaaa cgaaaaggag
     8761 tgaaacgaaa atgtatggtt atggaatgta tggtttttta gatccgacat ttcttttggt
     8821 cattcttgga ttagttattt ccggaattgc gtcagcgtac gtcaatagta ctttccgaaa
     8881 atacgatcaa gtgagaagca aaaacaatgt gactggtaca caagcagcac aatatatatt
     8941 acaaagtcaa cagataaata atgtcggtgt tcaacaaatt gccggagatc tcacagataa
     9001 ctacaattca ggtaataaga tgttgagttt gtctcaagca actgcacagt ctacttcagt
     9061 tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg gtacaagatg cttcgggtta
     9121 tgtcccattg aagattcgtg cagcacttgt tccagcagct aactttggtt cgatgatttc
     9181 gttccccttg attatggtag gtgtattgct tagctggaac tctacgttaa tcaatatcgg
     9241 gattttcgct ttttctttag cattactttt ccaattggtg acattaccag tcgaatttaa
     9301 cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga ctactgacgg aagaagaagt
     9361 accgatggca agacacgtat tatttgcagc agcactgact tatgttgcag cagcgctttc
     9421 gacattcctg cagctgttgc gcttgatctt attgttcggc ggcaaccgaa gagattaaaa
     9481 taaagaagtt gtgaaatcag cgctttgatc tgagctaata ccgagagtta gctgattgaa
     9541 taccatttat ttggaataag aggatgtgac aagaattttg tcacatcctc tttgttttgt
     9601 tctcataacc ttttttcata tacagttcat gaaccgcttt tattaacttg ccaaataaca
     9661 ctgtattgtg cgttgtatcc agatagaaag tctccgtttc atctgaaatt ttctcatgct
     9721 gatctgtaat gatcaaatca gcatgttctg aatatgcggt aattttaaca gcatctttgc
     9781 gaaataactg ctgcaaatgg ctttgaagat gataatttcc tgtaaactgt ttggttatct
     9841 gcaaatggat gagtaactgc tctttcgttt ctgtttgtag taagtgttgg atcaattgac
     9901 taaggtattg actgatttgg tttctttccc cattttttga aaaaattgta tcgatttttt
     9961 ctcttaattt cttttcctgt ataaaagaac tagaatgatt ggacgtggct tctggaaagc
    10021 aaagtgtaga aaaactactg ctggattcac ccaacagttc gaaaaaaaca actgccaaca
    10081 caagaaaata aaaataaatt ggtattttat tttctaaatg gttgagagga aactgttgag
    10141 ttaagtaagt catcaattga ccgacttgtc gacaaataag gtttttgctt gtggaaaata
    10201 aaaaaccaat ctcctgtttt tgtcgaaaag agatttgatt tggaaaaaaa caatagaaaa
    10261 aataattaaa gtaggctctc tctgtttctg tctcttcaca agggcgactc actgaaaaag
    10321 aagtaaagta ttctgaataa tcatgtgtat gctgcaataa gtccatcaat tcagtgattt
    10381 cagaagaagt ttttggaaaa tatgcctgtt gtttgtttcg taatatgaat aaagttaaca
    10441 gcaagtagat atttttttct tgttttgaaa ttttttctat ttgctcatct gtactgttca
    10501 tttttagtgc atttaattca agtgaggaaa atggccaatc gttacctcca aatgtatctg
    10561 aaagaaaaaa gtaaagatag agacggatag ataactcatt tccttcaaga gtacagcaat
    10621 tttttacaga taggatccgt aaatggaatt ttattaagga atgattgagc tgttttgtca
    10681 gtttttcgag ataggagggc gatatataaa gttgttcaag taaatatttt tttggtacag
    10741 cagtttgtgg ataattgatt agtaacatca aatagttaaa acgattggat tcttttaggt
    10801 agcataattt taacttagaa aaaacagcta actttttagt ttgatcggtt tctttggttt
    10861 ggatctctcc tttatgatta gaataaataa attcatcaat caataaaaat tgcttgagtg
    10921 atacatttat ttttcggatc agttctttga ttgtccgttt tg
//
LOCUS       DAZUMI010000102        10318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000102 DAZUMI010000000
VERSION     DAZUMI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.104"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACG81C_002248"
     CDS             complement(71..904)
                     /locus_tag="ACG81C_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5065390.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACG81C_002249"
     CDS             complement(1208..3373)
                     /locus_tag="ACG81C_002249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5065391.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACG81C_002250"
     CDS             3633..5927
                     /locus_tag="ACG81C_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5065392.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG81C_002251"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG81C_002251"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5065393.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACG81C_002252"
     CDS             6578..7585
                     /locus_tag="ACG81C_002252"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5065394.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACG81C_002253"
     CDS             7607..8626
                     /locus_tag="ACG81C_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065395.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG81C_002254"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG81C_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5065396.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3198 a         2242 c         1909 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DAZUMI010000103        10283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000103 DAZUMI010000000
VERSION     DAZUMI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.105"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2688
                     /locus_tag="ACG81C_002255"
     CDS             <1..2688
                     /locus_tag="ACG81C_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5065397.1"
                     /translation="KYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTGNMS
                     SVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINE
                     AFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQI
                     IEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFLDEV
                     HRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRRIQI
                     SVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGKLKN
                     IIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTDRIK
                     PFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKV
                     WVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLE
                     HLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYV
                     FEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLL
                     LNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLG
                     ISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSFSI
                     PNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIV
                     MSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDED
                     EFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2753..3253
                     /locus_tag="ACG81C_002256"
     CDS             2753..3253
                     /locus_tag="ACG81C_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065398.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            3265..3759
                     /locus_tag="ACG81C_002257"
     CDS             3265..3759
                     /locus_tag="ACG81C_002257"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5065399.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3780..4589
                     /locus_tag="ACG81C_002258"
     CDS             3780..4589
                     /locus_tag="ACG81C_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5065400.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4586..5407
                     /locus_tag="ACG81C_002259"
     CDS             4586..5407
                     /locus_tag="ACG81C_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5065401.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5458..7557
                     /locus_tag="ACG81C_002260"
     CDS             5458..7557
                     /locus_tag="ACG81C_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5065402.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7554..8672
                     /locus_tag="ACG81C_002261"
     CDS             7554..8672
                     /locus_tag="ACG81C_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5065403.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8711..9589
                     /locus_tag="ACG81C_002262"
     CDS             8711..9589
                     /locus_tag="ACG81C_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5065404.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9735..9932
                     /locus_tag="ACG81C_002263"
     CDS             9735..9932
                     /locus_tag="ACG81C_002263"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5065405.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            9916..10146
                     /locus_tag="ACG81C_002264"
     CDS             9916..10146
                     /locus_tag="ACG81C_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065406.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         3156 a         1681 c         2289 g         3157 t
ORIGIN      
        1 aaatatagaa tagacatatt gcagccgata gtttattatg cttttagaca cattaaactg
       61 gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg atgagctgat acgactaaca
      121 gggaatatgt cttctgtcac tgccgagaaa atttcgaaga atctaggtat ctctcgtcag
      181 aatgccagcc attatttgac gagattagtg gaagataaaa aggtcgagaa attaccggga
      241 aagcctgttt tgtggaagcc tttagatgaa tatgcggttg tagataatac ggagcaaata
      301 aacgaagcct ttcattctgt agttggtcat gacggaagtc tgagagaggt cattcaaaaa
      361 tgtatagccg ctgtcaaata tccgcctaac ggactcagtg tattaataaa tggagccacg
      421 ggtgttggaa aaagtttttt agccacaaaa atttttgaat acgctatcca tgagcaaatt
      481 attgaaaaag atgcaccatt tgctatctta aactgtgccg attatgcgga caatcccgaa
      541 ctgctttcgg caacactgtt tggatacaag aagggggcct ttacgggggc tgaaaaggat
      601 accgatggtt tattggcaac ggccaacaac ggctatcttt ttctcgatga ggtccatcgc
      661 ctatcgaaag aaaatcaaga aaagctcttt ctatttattg atactggaaa ctatcgtcca
      721 gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt ttattttcgc cacgacggaa
      781 aaaggtgaaa attatttatt agacaccttt gatcggcgta ttcaaattag tgttatgttg
      841 ccaacttttg aagatcgccc cattcgagaa cgattagagc tgattcaatt attttttcaa
      901 aatgaagcgg atgttttaca aaaagatatt attgtttcaa gagaagcctt gtctttaatg
      961 ttatctcatc cctttagtgg gaatattggt aagctgaaga atatcattaa aatcagttgt
     1021 gcggatgtgt atagtcgaac acaagaagaa ccattaacga ttgggaaaaa tgagatcttg
     1081 attcagttaa atatgctgga atccgaagta gaaagcttgc cgattgctag tttattgata
     1141 aagaaaaatc aaaagataaa aacggatcga atcaaaccgt ttgattatga ttcgattttg
     1201 gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg aaaatttttc ttcgataaag
     1261 aatgaagtac agcatttggt gcatcacatt gataaggatt cttttttatc aatgagtaga
     1321 aaattgatga ttcagatgtt tagaaaggtc tgggtatccg ttttaggaaa aacgtacgga
     1381 ttgacgacga gtggacctgt tgcagaaatt attagcagat cctatgtcat ttccccaagc
     1441 tataagacag acttaaccgg gataaatcgt acattagctg caaatctacc tcggacgact
     1501 tacttatgta atcagttctt ggagcattta ccaccgctct cagaaaagga cgagaacttt
     1561 ctaagactgc ttattgtcgt gactttatct gattatgtag atgaatccat cgagctgaag
     1621 ggcctgttgc ttgctcacgg agatagtacg gctagcagca ttcaagcagt agtgaatcaa
     1681 ttatgcggca attatgtttt tgaagcgttg gatatgccag tagagagtaa tgtgtcagag
     1741 attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga atacagatag tctagtgctg
     1801 atcgtagata tgggctcttt aaatcaaatt tacacacaga tcaagaacga tttaaaaggg
     1861 gagttacttc ttctgaataa tttgacgact tccattgcac ttgatattgg cttgaagatg
     1921 acgatgaatg cgccatttaa acagattgcg gaggatgcag cagacaagta ttcaatcaat
     1981 gttcaatact ttgaaggctt ttctcaaagt agcaacatta tcatttcctg tatgtcgggt
     2041 ttggggattt cggacaaatt gaaagatgtt tttcgtcatc atttatcgga tgaatccatc
     2101 gaagttttca cgaaggacta cagagaacta cgtgaattaa tcgaccaaaa tgatgaaact
     2161 tattttgaca agacaaaatt ggtcatcacg acttcggatc ttccacgttc cttttcaatt
     2221 cctaatatta atgtttatga catcttagac ggtgatggag cagccaattt ggagcatatt
     2281 ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga ttcaggagct agtaaaattc
     2341 ttttcgttgg aaggaattgc ggaccgtttg aactttctga acccaacgat cgtgatgagt
     2401 gaagtggaaa cagtcatttc taaatatgaa aattactatc atttcacttt aaatggccgt
     2461 gtaaaactaa atctctatat gcatacggca ttgatgatgg aacgtttatt cttggctcgg
     2521 acagaaaaat ctgaagaacc gaattatgat ttgaaaccag atgaagatga attttattat
     2581 gtttctagaa gtatttttca atcaatggag atgaaatata attttaaagt caatgcttat
     2641 gagctttcgc tgctctatga attattagga ccgtatattc aaaaataaag tgtctaaaaa
     2701 agtgtctaat ctgttttttt taggttagac acttttttta atgtgaatta acgtgattaa
     2761 accaatgttt ttaaaagttg gcacaggaat tgcttatata aaagtgaaca gaaagaaagg
     2821 aattaaccat atgaagaaaa ttctaatagc gactcacggt cacttagctt caggctttct
     2881 tagcagtatt caattactga cggggaaggt tcaagagatt acggtgatta atgcttatgt
     2941 ggatgagtgt gattttgaac aggagttgaa tgtatttgta aaccaggtga acgagcagga
     3001 tcagattttt gtttttaccg atttatttgg cggaagtgtc aatcagaaga ttaccaaaac
     3061 ctttttggag aaagagattg ctgtaactat tttgtctggc tttaatctgc caattctgct
     3121 cgaaatagta ttggcaacca aggaattaac aaaggaggat attcaggaac tggtaaataa
     3181 atgccaacag gaattaaaga tgaccattct tgatgaggta catgtagtaa atgaagagga
     3241 agattttttt taggaggaag aaaaatgatt aaagcagtgc gaattgatga acgattaatt
     3301 catggacaag tagcgatgac ttggacaaag agtctaaatt taaccgggtt agtagtagca
     3361 agcgatgagg cggctaccaa cgatattcaa aagatgacat tgaagatggc tgcaccatcg
     3421 aatgtcaaag tcattattaa aacagttgat gacgcgattc atttgttaaa agatccgcgg
     3481 gcgaaggaca tgcgcttgat ggttttagtc cctaatgtca aagatgcggt aaaagttgcg
     3541 aaagctttcc caacggaaat cgaattaatg aacgtgggga atgctgggaa aatgacaccg
     3601 ccatcggaag ataagaaaac cttatcaaag gaagtgatgt taacgacgtc agaaattcaa
     3661 gacttaaaag aattagttga actttatcca gataccgttt ttcagcctac accggcgatg
     3721 gaaaaacgtt cagctatctc tattttgagt gatttttaga aaaaggaagg agaaaaatta
     3781 tgtatgatgc atttgtagtc gctttggccg tttttattgg agttgctgga catgaaatgt
     3841 tcgggatgtc catgctgagt cgtccgattg ttgttgctcc gttagcagga gcgttgatgg
     3901 gggatttgca gacggggttg attgtcgggg cgtcactaga agccatcttt atgggtgtcg
     3961 tcaatatcgg aatctctagt acggcggagc cttctttggc tgctgggctg gctacagcgt
     4021 tcgcaatcaa aatgggagga aacgtggatg cgattattcc cattgtcttt ccactagcag
     4081 tattaggatt acagttaatg aatatgattt tttcttttgt atgtggaccg atggctgcta
     4141 aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat catcaatctg cacttcttta
     4201 tgtggtttgt ccactatgcg ctttatgctc ttattccatt ctttgccgtt ttatttggag
     4261 cagatgttgt caaaagcgtc ttggataata ttccagaggt catcatgaac ggattaacag
     4321 tcgctggaaa tctgctgccg gcagttggga tggcgatgct gttacggatg ctttggagta
     4381 aaaatattgc tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc gagttaccat
     4441 tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa agagatctac
     4501 aactaaaaaa tatatctgtt catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg
     4561 accaagaaga ggaggatttc tttcaatgaa attagctgat ggaatttcta aagatgagaa
     4621 aaaattggtt cggaagatgt tttggcgctc gatgactatg tatgcttcgg tgaatccggt
     4681 gacgatgggc ggcggtggtt tcgcctattc tatgatgccc ttcattaatc atttctacaa
     4741 aagtgaagaa gatcgagctg cggcgctgga acgtcataca cagtattttt cgacgaccat
     4801 tccttgcgcg tcctttgtca tggggattgc ggcatcgatg gagaaggaaa attctgaaaa
     4861 agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg gaccattggc
     4921 tggaattggc gattcattgt tctggggagt ttggcgtgtc gttaccgcgg gactagcgat
     4981 caacttagcc aatgccggaa atattttggc accaattatc tttttagtta tgttcaatat
     5041 acctaattac ttgatgcgat actatggcgg cgttttaggc tattcattag gctctaaata
     5101 tattgaaaag ctgtatgcga acgggttaat tgaaattctg acaaaagctg ctagtatctt
     5161 gggtctgatc atggtaggtg caatgacgat cagtatggtc aaattcaata ccgttttgag
     5221 cttttcaatg aagggcgaag aactaatgaa tgtccaagaa atcctcgata gtatttttaa
     5281 aggaattgta ccgttattgg taacattgag ctgtttcaaa ttactagata aaaaagtcgg
     5341 cgtcatcaaa attatttttg gaatggtcgt cttgggaatt ttattgtcat tacttggaat
     5401 cgcatagtag gaagctgcag ctactaggct gcagtttttt tagcaaggag ggaaaagatg
     5461 actattaatg aagtaattta ttcacccaaa cacccatttt ttaatgctga gttaaacgac
     5521 ggaaatgtga ctacttcaaa cgaatacagc tactatcgga atattcctga tgtaaaggga
     5581 ttgaccttcg atgtaagtaa tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt
     5641 caaacgttga ccttttatcg agaaaattta ctaactgagg aaaaaccagg tgtttgggtc
     5701 aataaacagc tctctcaaac caattcgtta tccttaaagg ttgaagtaaa cgggattgtc
     5761 taccatttgt cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct
     5821 agatatattc atcattattc gacgtttaag gtcattgtag tatcctttgc tcccatactg
     5881 gctaataagc gtttaagtat gcttgttcaa caggtttaca ttgtgaatga aacacacgaa
     5941 agccttcaag tttcagtgaa agatgtgcct tcgtatcaag agaagtattc ggatcagcaa
     6001 aatgtgttga tttctcaaaa aggaaataag gacacgatcg cacaacagga agtggcaagc
     6061 ttcgccattg cttttgtgga cccaaatgct tttgaagaag taatgacttt tcaggaatct
     6121 gatatcgaaa aatggctgaa ggaaacattg aactattttg ctcagctatt tgggcagctg
     6181 tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg cactttatca aagcttttct
     6241 agtttcggta tgaatcgaca gggacaggtc gtcggcagca attggggcag ctatcctgct
     6301 actaatcgaa tttggaataa ggatatgtat tacgcatcat tgccattcac catgtttgaa
     6361 ccggagttat gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa gtttcctggt
     6421 tcaaaattta caggaggtat caatcattct ctgagtaatt cgttatccag tgcgttactg
     6481 gctagtcttt attttgaaca tactggggat acaaaattct ttgagcggtg tccgcaggtt
     6541 ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc aggggaacca
     6601 atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta tcataccggc
     6661 agcaatcttt gtctttggaa ggcctgtatt ggacttgcag aaatttttga agccctagaa
     6721 caactgagtc ttagcaagcg atacaaaaac attgcctgta aactaaagga atcaatttgt
     6781 aaacaaatga ctatcgatgg tccttttggt gagcaatttt tggagggaat cggtgatgag
     6841 gagaaaacaa cctattcagt gaagcactat caaaaaccga ttcttgagca agggttaatc
     6901 tttttaagtg atgtgattgt ggatggaaaa attaatttat tgatgcatga tggagaagaa
     6961 tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatca tttatatcag
     7021 aacaccatgg ccttttctgc tagttctttc aatccaacct atagcgagga aatcaaaggc
     7081 atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagc
     7141 gagctgcaca accctcaagc tttcgtaacc cgattagaag aactaatttg tttggcagat
     7201 ttagacggtt cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg
     7261 cgagactttg gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag cctaatgatc
     7321 agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat
     7381 ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg
     7441 caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa
     7501 aaaattcttc acgtagaaaa gggaaaaacg attcacattc aaaggagaaa aagatgagta
     7561 tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc
     7621 caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca agccgcaaag
     7681 gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat attactggaa
     7741 cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt ggcagttttg
     7801 ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagccct gctgttgaaa
     7861 gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc
     7921 cgatagtggt taatcctaca gggggcttta attcctattt cgaaatgcct ttccaaacat
     7981 ccgctaaaat tacgatcgag aatttacatc cgaaggatat gtcctcattt ttttatacga
     8041 taaattacgc tttggtggac gaattggaag aaaatagcct atattttcac gcaaattggc
     8101 atcgggaacg cattaccaaa ttacaggagg attataagtt aattgatcga ttaaagggtc
     8161 aaggctatta cgtgggtact taccttgcgc ttacctgcct agaacgttat tggtggggcg
     8221 aaggcgagtt taaattctac attgatgggg atagtgagta tccgaccgta acctcaacag
     8281 gttcagaaga ttattttggc ggggcgtggg cctttcatga gtacgatgag ttcggacgtc
     8341 cacatgcgaa aaactattca acagcctttt tagggtatcc ttaccaatca aatagagatg
     8401 ccacaagaga tcgttttgaa acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg
     8461 caatgccacg acatggtctt tatcgttggc atcttcaaga ccccatctgt tttaccgaag
     8521 atttatcggt cactttacaa caaatcggca acgatgatgt tcgcttattt gagcggcaag
     8581 acgatgttgc tagtgtcgct tactggtatt caacagaagc ttcaggaaat actaaacgtc
     8641 atttcactga tcgagaacgc ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga
     8701 ggaacctatg atgatatatg gggcaattga agctggaggg actaaatttg tctgtgcagt
     8761 cggtactgag gaattaacga ttttggaaag agtcagtttt cccacaacaa ctccagttga
     8821 aacaatgaag caggtcattg atttttttaa accttttaag gaacaattgg caggcatcgg
     8881 ggtcggctgc tttggtccaa ttgatattca atgcaaatca acgagtttcg gtcatatcac
     8941 atcaacacca aaacttgctt ggcaaaattt caattttgta ggaacgctaa agcaatattt
     9001 caatattccg attgaatgga caacagatgt caatgcggct tgctatggtg aatatgttgc
     9061 tggttctggc aaagggttag caggtgttgc atacttcact gtgggaacag gtatcggagg
     9121 tggtgccttg ttcaatggga ctttcgtgga agggttcagt catccggaaa tggggcatat
     9181 gttgatcaag cgacatccaa aggacaattt ttcaggaaac tgtcccttcc accaagattg
     9241 tttagagggg atggcagcag gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa
     9301 tttattggct gacgattcgt tttggcagat tgaagccttt taccttgccc aatgtgcata
     9361 caatacaaca ctgatgtttt cgccagatag aattattttt ggcggtggtg taatgaaaca
     9421 agaacacatg aagaaaaaag ttcaggataa atttgttgag cttataaatg gatatgttga
     9481 aataccacca attgatagct acattattac gcccgaatta ggtgataatg ccggaataat
     9541 cggcggatta gcattggcca gaaaagcggt aagaaataag caaccataat taatgaatgt
     9601 ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg
     9661 ataaagcgaa aatgaattca aataaaaagg ctctaagtca aatcgtgttg ttttcattaa
     9721 atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct
     9781 tgaggatatt cactggtggc gtacacttaa atagtaatat attgtgttac tttatgtctg
     9841 tggtaatagt tatagttgct taccaatgta tgttcatttt tcgaaatgtt gacttcataa
     9901 tccttggctt aatgtttgaa tttttttggt gatagatttt ttcagccgta taaaataaat
     9961 cgtaattcta ttatagagag ttgtgattat aaaaagatag caagaaaaaa aaagaaagtt
    10021 tctatacatt ttaaatattt tattcctatt tctattgcta aaaaagtaga aattctatat
    10081 ttacctttaa ctctcgcttc tcttgcaaat gtagttagca ttttaatcga taataaactc
    10141 aggtagttat attaccttga aatgattgat aattttgtca cttctatttt ttttgcaata
    10201 aattttttat tttagtgaaa aataagaaac tatttctttg aaggcgataa gttagaaaaa
    10261 tattattgta gtcttttctt tat
//
LOCUS       DAZUMI010000104        10237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000104 DAZUMI010000000
VERSION     DAZUMI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.106"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..249
                     /locus_tag="ACG81C_002265"
     CDS             100..249
                     /locus_tag="ACG81C_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5065407.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            260..1774
                     /gene="dltA"
                     /locus_tag="ACG81C_002266"
     CDS             260..1774
                     /gene="dltA"
                     /locus_tag="ACG81C_002266"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5065408.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1771..2976
                     /gene="dltB"
                     /locus_tag="ACG81C_002267"
     CDS             1771..2976
                     /gene="dltB"
                     /locus_tag="ACG81C_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5065409.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3021..3254
                     /gene="dltC"
                     /locus_tag="ACG81C_002268"
     CDS             3021..3254
                     /gene="dltC"
                     /locus_tag="ACG81C_002268"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5065410.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3257..4525
                     /gene="dltD"
                     /locus_tag="ACG81C_002269"
     CDS             3257..4525
                     /gene="dltD"
                     /locus_tag="ACG81C_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5065411.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4666..5748
                     /locus_tag="ACG81C_002270"
     CDS             4666..5748
                     /locus_tag="ACG81C_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5065412.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5748..6551
                     /locus_tag="ACG81C_002271"
     CDS             5748..6551
                     /locus_tag="ACG81C_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5065413.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6621..7361
                     /locus_tag="ACG81C_002272"
     CDS             6621..7361
                     /locus_tag="ACG81C_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5065414.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7372..8376
                     /locus_tag="ACG81C_002273"
     CDS             7372..8376
                     /locus_tag="ACG81C_002273"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5065415.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8421..>10237)
                     /locus_tag="ACG81C_002274"
     CDS             complement(8421..>10237)
                     /locus_tag="ACG81C_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065416.1"
                     /translation="KESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIK
                     DSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITV
                     YLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLG
                     TTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALAL
                     GFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQL
                     LIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIV
                     LVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFL
                     KIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAK
                     NFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALE
                     HSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFD
                     LMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3284 a         1698 c         2073 g         3182 t
ORIGIN      
        1 aaactgttag taaaaaaacg gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt
       61 agttacactt tgtgtgaggt tatatgagga gggaatccaa tgaacaaatg gcgttctaat
      121 ccaaaattga attattgggg aaaattcatc attcgaacaa ttttttatag tgcagtactg
      181 ctgttcctga tttatctgta tcactataaa aatattcaag gaggaacttt tatctataac
      241 gaattttagg aggaagagta tggaaatcaa aacgattatt gaagcaatcg atgattgggg
      301 aataaatgaa cctgatcgag ttgcttatca ggcggatgag acgcatactt ttggtgaatt
      361 gaaacaagca tcagacgcac ttgcttatta tttagaaaaa aaggtagaag gcgatggacc
      421 aattgtcgtt tttggcaatt tggagtttga aatgattgtc tcatttctag gggttgtcaa
      481 atctggccat gcctatttgc cgattgaaga gcatacaccc aaggagcgta tcctatcgat
      541 ctttcgggtg gctaaaccat ctatggtcat cagtatcggg gactggatag aagatattcc
      601 ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc aagaagatac cagtaggatt
      661 ttatcccaga aatagcgtaa aaggtacaga aaatttttat attattttta cttctggtac
      721 tactggagaa ccaaaaggcg tacaaatcag tcatgataat ctagtcagct ttgttcaatg
      781 gacgatggaa gactttggga tcaagcctgg tatgcatttc ttagcccaag cgccattttc
      841 ttttgattta tcggtatttt ctatttatcc tgcattagtt tctggcggaa tgctgaagcc
      901 gttaaataaa gcagttgttc aagactttcg tcaattattc gcgacattgc cagccttgaa
      961 gttgaatgtg tgggtttcta ctccatcatt tatggatatt tgcttgatgg aacctacatt
     1021 caatggagaa aatgtgccgg aattggacat gtttttattt tgcggagaag aattgacgaa
     1081 aaaaactgca gaaagtctac ttgatcgatt cccaaatgca cggatatata atacttacgg
     1141 tccaacagaa gcaactgtag ctatttcaag tattcagatt gaccagcatg tactagatag
     1201 ttatgaccga ctaccgattg gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga
     1261 aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt attgcaggac caagcgtatc
     1321 aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt
     1381 agcaagttac catacagggg atgcaggtag actagatgaa tatgggcttt tattttatga
     1441 aggacgtatg gatcagcagg tcaaattaca tggataccgg attgaactcg gggatatcga
     1501 acattatctg ctccaagaca accgaatcaa acaagctgtc gtcgtaccaa aataccaagg
     1561 aacaaaagta cagcagctgg ttgcttttgt cgtattagaa aacaaaacga aacagccgga
     1621 ttttcagttg actaaatcta tcaaagaaca attactagaa gttgtaatgg attatatgat
     1681 tcctcagaag ttccattatg tcgatctgct gcctcagaca gttaatggaa aaatcgatcg
     1741 aaaaaaatta acagctgagg tgaatcctga atgatgttac ctcatatgat cccttatgca
     1801 gagccgtttt atttttttct tttagccatt gcgcttgtac cgatcgttct atctttattg
     1861 atatgggaaa aaagattacc agtttatcaa tcagcagtga cacttttctt tttatttgtc
     1921 agctttggag gtaattattg gcaacaggga gcggcgctga ttgggtatgt tttgtggcag
     1981 acgatacttg tatggggcta ttttgtttat cggcaaagga aaaatcagac aggatggttt
     2041 tttgccgccg ttattttagc gataattcca ctgtttttaa cgaaagtgac tccttttttt
     2101 gaacatggaa aagcatcgtt acttggtttt ttaggaatct cttatttaac ctttaaatct
     2161 gtacaaatga tcatggaaat aagagatggc atgatcaaaa catataagcc atttcgttat
     2221 atccagtttc tgctgttctt tccaacaatc tcttctggac caatcgatcg ctataggaga
     2281 tttgaaaaag atcagctcca tccgcctgtt cgtgataaat atgttgatct tttgggaaaa
     2341 ggcgtccaaa atatttttgt cggtttttta tataaattca ttatcggtta ttattttggt
     2401 caagtactgc ttcctgtagt gggacgcatt gccttacagc atggtggtat ttcatgggca
     2461 ttagtaggtt atatgtacgt gtacagtatg tatttgtttt ttgattttgc tggctatagc
     2521 ttatttgcag tcggtacaag ctacatgatg ggatatcaga caccaatcaa tttcaataaa
     2581 ccttttttat catggaatat caaagaattc tggaatcgtt ggcatatgac gctgtccttt
     2641 tggttccgtg attatgtata tatgcgattg atgttcttca tgatgaagaa aaaatttttt
     2701 aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt tgtttcttct aatgggtata
     2761 tggcatggat tgacgtggta ctacatcgta tatggactgt atcatgcttg tctgatctgt
     2821 ctgacagatg catggatccg atttaagaag aagcataaag agcagttgcc ttctaacaaa
     2881 tttacgcatg gcttagccgt atttttgaca tttcaagcag tatgttttag cttcctgatt
     2941 ttttcaggat ttttagataa actttttttt agctaacgaa tagtgacata ttaatttata
     3001 taacaaagga gaaaaggaaa atggaagaac aaattttatc aatcttagaa gaaataacag
     3061 gaacagatga agtaagaaaa aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt
     3121 ctttagcatc tgttcaatta ttagtcgagc tagacggaga gctggatatc caagtccctg
     3181 tatctgaatt cgagcgagag gattggtgca caccgaataa aatcattcaa caagcaactg
     3241 cattgaaggg ttgagtatga aaaagaaact ttttgctatt ttcggcccgg ttgttttagc
     3301 tgttggctta ctgtttgtgt tttttacctc ctcctatcaa gtagatatct ctaatccaga
     3361 aacgattaga cgtgcctctt cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa
     3421 aaattatgca ttatctgaaa aacaatatat tccttttttt ggctcaagtg aattaagcag
     3481 aatcagtcca tttcatccgt ctgttctggc agaaaaatac cagcgaaact atcgtccatt
     3541 tttgctggga gcacccggga cacaatcgtt aagccaatat atgatgatgc gttctgcagg
     3601 agatgctatg aaaaacaaga aagtcgtctt tattatttct cctcagtggt tcgttaaaaa
     3661 tggtgtaaaa actgattact ttaatacgta ttattctgaa cttcaaacat acgactggtt
     3721 gttttcaatg aagaaagtaa cgcctgctga tcgctattta gctagaagat tattaacttt
     3781 ctcaaaagta aaagagaatg atacgttgac ggcgattctg caaacgatca aaaaagggaa
     3841 gctaccttta cctgaatctt tgaatcagct gcgcagccaa tggaacatgc tgaagagaga
     3901 ggatgaagta ttcagcaata ttgggttgaa agatcgacag caaaaaatcg atcatgagtc
     3961 aaaacgatta ccaaaacaat accaagaaac agaattatcc attttagcaa atcaaattgg
     4021 agaacgagaa acgacgaata atccattcgg gttgaaaaac gatttttata cgcatcgtat
     4081 ccgagcccac gagccagagc tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc
     4141 agaattttct gattttcagc tggtcttgga tcaattagca aaaaaccata acgaagtatt
     4201 gttcatcatc ccaccggtta acgagaaatg gagcgattat accggactat ctcaagaaat
     4261 gttacaagga tttgccaaaa agatcaaatt tcaattaaat agccaaggat tcaatcgaat
     4321 agctgatttt gtcaatcaag cgggtaccaa ctattttatg gaagacacga tccatctagg
     4381 ctggaaaggc tggttggctg cagatcaaca gatcagaccg tttttagaag aaaatcatat
     4441 tactgctagt aagtatcatc ttgacgatgc tttttttagc aaatcttggc aacatcaaat
     4501 accagataaa ctacaattaa aataaagaaa acaggcgagt gttgctcgct tgttttcttt
     4561 atttttgaat attctgaata aaagataaaa ataccaagtt tgcctgaaca aaaatcataa
     4621 gcaatggtat aatcacaata tgaatataga aatgggggaa aacatttgga tacaaaagaa
     4681 gaacagctac ttattgattt aacttctgca aaaggagtac ctggaaatga agaagaggta
     4741 agagaagtat tcagagaata tgccaaacca ttcgctgatg atatttttta tgacggttta
     4801 ggcagtgtga ttgccaaaca tggtgctggc ggaggcccca aggtctttat ttctggacat
     4861 atggacgaag ttggttttat ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa
     4921 actcttggag gttggtgggg gcaagtcatg cttgctcagc aagttgaaat caaaacacgt
     4981 gaagggaaaa tcgtccacgg tgtgattggt tccaaaccac cacatgtttt aacacctcaa
     5041 gtaagaaata aaccttatga aataaaggat atgttcattg atattggcgc atcaagccaa
     5101 gaagaagcca aagagtgggg catacgtcct ggagatatgg tcactccata tattgaatac
     5161 aaacgaatga atggcagtaa atatttactg gcaaaagcat gggataatcg tatcgggaca
     5221 gctgtttctc tgcgagtatt agagaatcta tctaaagaag cacacccaaa tgttttattc
     5281 gcaggaagcg atgtgcaaga agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt
     5341 gtaaatcccg atattgcttt tgctttagat actggtacag caggagacac tccaggaatg
     5401 acaccaaaag aagcagattc gattttagga aaaggcccac agattttgat ttttgatgca
     5461 tctatgatcc cgcataaaaa attattgaat tttgttattg atgtagcaga ggaaatggat
     5521 attccattcc aatatacagt gatttctggc ggaggaactg atgcaggaca aatgcatttg
     5581 acaagagatg gtgttccgtc tgtagccatt accgttcctg ttcgttatct gcattcccat
     5641 acatcagtga ttcatgaaga tgattatttg aatacagtga aactagtcac agaattagtg
     5701 aaacggcttg atagtgaaac agttgaaaaa attcggagtt atgaataatg aataatagaa
     5761 tacctatttc tgaatatcgg aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca
     5821 atacttggct tgttttctta tctataatat ttacgagcgt catccagaac ttttttgacg
     5881 gtttcagtcc ttttccggaa gaaagcatgc agcagcactt attgagtttt ctagtcgata
     5941 atttactttt attcgcattc aactatgcat tgttttacat tgcgctagta gttgtacggg
     6001 gtggcagacc aaaaagtaac cttttatttt cagttttcca aagaaagtat tttggttcga
     6061 tacttggcat caatttgatc aattcagtca tcaactggct actcaacagt ttaatctttc
     6121 tgcccatgtt tttaatcagc ggagtgagta cttatatgca gctgcttttc agttcaaatg
     6181 cggtacctac gggtgtcatg ccagaagatt ttcttgatat cagctttgtt ttatcgatga
     6241 tagggatgat ttttgtttca gcactcatca tgagtattgt tggcggttta ttccaatttg
     6301 ctgcatggac aaagttcgac tatcctgatt tgtcggttat tcaatgtttg aaatatggct
     6361 ggtttttatt gaaagataga attggtacgt atattttgtt gcagctgtct tttatcggct
     6421 ggtatttgct aggagcaatc gctttgttca tcggtctttt gtgggtcatt gtttatgtga
     6481 atgtgtcagt tgcagaattt tatgaacaag cacggattga aaaaggaggt ccagacgcct
     6541 attttactta gcatgtaagc agtttattca tcaaaaaaat aacttttgtg gtaggataaa
     6601 tctttgaggg ggaatgtacg atgaaattaa ctgttttagg atgcttagga gcgtatcctt
     6661 ataaaaatca agggacaaca ggttatttgc ttcaatctga cgggttcaat ttgttgatcg
     6721 atgcaggtag tgctacgctg atcaaattac aagaacacct tgatccactt gatctagatg
     6781 ctgtgatcct tagccattat catcatgatc atattgctga ccttggtgta ttacagtatt
     6841 actggcaact tcatcctaag agaaatccgg agacattgct ttcaatttat ggacacacaa
     6901 aagattccat tcactttgaa gaattgacgt tggaaaatgt ctcaaaagga catccttatt
     6961 ttgaagctga agaattaaaa attggtcctt ttctgattac ttttatggaa acaatccatc
     7021 cagtagtatg ttacgccatg cgtatcgtag aagaaacaac ggggaaagta ttcgtattta
     7081 caggtgattc aggttatttg gaaagtttcg ctgattttgc gaaagatgct gatttgtttc
     7141 tagcagatac ctatcttttt gaaggaaatg aacatcatca tgcccatttc acttcaaaag
     7201 aagctggaga acttgcgaaa tcagctaacg taaagaaact tgtacttaca cacctacctc
     7261 agtttggtga tttagaacaa ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg
     7321 atatagaact tgctgcagtt gacaaagtgt ttaacattta ggaggaagaa catgattttt
     7381 gtaccaaatg aaaaccacga cccaagaatc aatttagcaa ttgaacaata tttgctgcaa
     7441 gaaatgcctt tagatgaacc tattttgctc ttttatatca atgaaccttc aatcatcatc
     7501 ggcagaaacc aaaatacgct tgaagaaatc aatcgagatt atgtggaaga aaatggcatt
     7561 catgttgtcc gtcgcttaag tggaggcgga gctgtctacc atgattttgg aaacctgaac
     7621 tttagtttta tcatgcctga tgatggggat tcattccgtg actttgcgaa agtaacacag
     7681 ccaatcatcc aagcacttca tgaattgggt gttgctgggg cggaattgaa aggccgaaat
     7741 gatctagtca ttgatggcat gaaattttct ggcaacgcga tgtatgctac caacggtcgg
     7801 atgtttgctc atggaacaat tatgttcgac tctgatatca atgaagtagt caatgcatta
     7861 aaagtaaaaa aagacaaaat cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg
     7921 aatatcaaac catttcttcc agaagaaaag caaaaaatga caacagaaga atttcgtcaa
     7981 gaaatcttgt tgaaaatttt cggcgtagag tcagtagaag aagtaaaaac gtacgaattg
     8041 actgatgaag attggaaaaa aatcaatcga atctctgagg aatattatcg aaattgggat
     8101 tggaattacg ggaagtctcc agatttcaat ttctctcgcc aaaaacgttt ttctatcggt
     8161 tcgattgaag tccatttgaa tgtctcagaa ggaatgatca aagaagcaaa aatctttggc
     8221 gatttcttcg gactaggaga tatctctgac gtggaaaaaa aattggttgg acagaaatat
     8281 gataaagcat ctctatcaaa aatagtcagc caaattgatg tgaagaaata ttttggtgcg
     8341 atagaaccag aagaattgct taccttactt tattgaaaat aaaaaatagc ggaatccaca
     8401 tatatggatt ccgctatttt ttatacagga ttcgggtaat ttcgagagag ttgaacagtg
     8461 atccgatcaa tgaaagtatc tttttcctgc gttagtgttc ctgtgtaacg atccgctacg
     8521 tctttcatca tcattaagtc aaaacgttcc tgatcatcta attcttctgt gatgcctcga
     8581 ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac aaatgacgaa atgttcctct
     8641 tgttcgtgta tcgacaaata aatttttcga tacagtggat tttcgatggc atgagtttct
     8701 ctaaaggaat gctctaaagc gtggatcaaa agttgagcca gatcgtcatc gttgatagga
     8761 agttcttcgg gaagatctgc ttggaaggtt atctgaatat tccgtttggc agctaacttc
     8821 tggtaacgcg ctaagataag atttgtcacc tggtggttgc aaaaagaaca cagcatttcg
     8881 aaattttttg cttcttctaa tatcttttcc acatagtttt tagcttttgt ttggtccgta
     8941 tgcattaaat cttgcagatt ctccatctct tggacaaatt ccaggcgaag tgtatttact
     9001 tcttggatat gctgtcgaag ctgctcataa tgttgctcca acaaatcatt tttgattttt
     9061 aagaaatcta cttgtctccg atctttgtcg gtatttaaga tgtgatcgat tagattgtag
     9121 ctgataataa cgattagaat gataatatat aaaatcgtag accaattgat cgttgtttga
     9181 tagataccta atgccgtttg gatgtaaatc aagcaatgga taatggctac caagacgatc
     9241 caaggtgtac aagctacata aaaacggtta ttcttatatg cttcgccaat gcatgcaagt
     9301 gaagtgacta aagtgcttat aacactagca atattagcaa tcggcattaa ttcaggaagt
     9361 tctgcaggag taaaagttgc ataaagcaga acgccgataa aaatcaaaag ttggaacgta
     9421 caaaaaattc cgtaccattt tttatagtaa aacattcggc tccaatagta gcagatgatg
     9481 aaagctgacg ttaagataat aaataaatga gataaaaaag aatgaacgaa tggttcaaaa
     9541 agaataccac ttaaaatatc ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa
     9601 cttaagagaa taagtgagta ttcaagacgt ttttgtttat acatcaagta gatcgtatag
     9661 aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg tcaatacttg cggcactgat
     9721 tgtgaataaa taaaactaat caatggtcca gttgttccta gataaacttt cggtggcagt
     9781 ccagcatagt tctgataagg agatgagact gtaagggtca atttttttcc gatataattt
     9841 gctggtaaat ctatcacagc caatgagagt ccgggattgc tttcttcctg ataatctttc
     9901 cgttggtata aaatcttttc gcctagatat acagtaatcc attgatggtt tcctttgatt
     9961 agtaaatttg cttcgtccaa cggtttttca agtgtcgttt ccataatgaa tgtttcgtct
    10021 ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa acttaaaatc aggatcatct
    10081 tttgtataga aaacccacga atcttttatt tctttcattt gattgctgtc cgcaggagaa
    10141 aaaataaaat aaaagaaata agcaattcct gttaaaaata acaatgataa tataatgatt
    10201 atgctccact ttatacgtaa ataaaaagat tcttttt
//
LOCUS       DAZUMI010000105        10164 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000105 DAZUMI010000000
VERSION     DAZUMI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.107"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..460)
                     /locus_tag="ACG81C_002275"
     CDS             complement(140..460)
                     /locus_tag="ACG81C_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5065417.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(608..2842)
                     /locus_tag="ACG81C_002276"
     CDS             complement(608..2842)
                     /locus_tag="ACG81C_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5065418.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3321..3587
                     /locus_tag="ACG81C_002277"
     CDS             3321..3587
                     /locus_tag="ACG81C_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5065419.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3587..5314
                     /gene="ptsP"
                     /locus_tag="ACG81C_002278"
     CDS             3587..5314
                     /gene="ptsP"
                     /locus_tag="ACG81C_002278"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5065420.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5478..6524
                     /locus_tag="ACG81C_002279"
     CDS             5478..6524
                     /locus_tag="ACG81C_002279"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5065421.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6541..7764
                     /locus_tag="ACG81C_002280"
     CDS             6541..7764
                     /locus_tag="ACG81C_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5065422.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7853..8740
                     /locus_tag="ACG81C_002281"
     CDS             7853..8740
                     /locus_tag="ACG81C_002281"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5065423.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8826..9056
                     /locus_tag="ACG81C_002282"
     CDS             8826..9056
                     /locus_tag="ACG81C_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5065424.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9113..10078)
                     /locus_tag="ACG81C_002283"
     CDS             complement(9113..10078)
                     /locus_tag="ACG81C_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5065425.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3225 a         1798 c         2057 g         3084 t
ORIGIN      
        1 gaaaagcaat tttcgtgaat ttcagcttat ttccgaagga gttgcttctg ctttcgccgt
       61 ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttaat
      121 tattatgttc aaaatttgtt tacacggcta atgtagcgcc taattcatgt tttggaatcg
      181 agatttcaac aaggcctgcc aattcgatta ctttgatttc atcctcgtgg taaacggcgt
      241 ctttgatttt atctaagaaa taatttttag catcttcgat ttctttcggc tggattttac
      301 gtttgtcatt gatcagcaga atttcattat gttcaggggc ttcagtgtag acaatagtgg
      361 tatttccggc tgtgtagacc ttgactagat gggcatcagt gttttcaagc tggtttaata
      421 caagacggga atggctgttt gttacattta ccaatttcat ggcgctcacc tcttttatgt
      481 atttttaatt gcttgtttct cttattataa tttctttttt ataaaatgaa cagtattttg
      541 acttttctga aaaaagaatg agacggcatt tctcaaagaa ataccgtctc acgctataaa
      601 actggattta ttcagccgtt tcttcggctg attctttagc caaacgttct ggtttagaag
      661 taacgatgat ttttccatct ttatccagtt ttgctttcaa ctcatggata gaagggtgat
      721 caagataaaa ttcagcgata ccatcttcga tttgttcttg gatcgtgcgt cgaagaggac
      781 gagcacccat agatggatca tagcctaagt caactaattt ttctttgaca ttcgtaggaa
      841 cttcgatgtg aagttgctgt gctgccagca tttggttgac atcgtctagc attagtgtga
      901 cgattgtcat caagttttct tttgataatg cactgaattc gatgatcccg tcaaaacggt
      961 tcagaaattc tggtgtaaag tagttgttta attggttcaa tacagaacga gtaacacctt
     1021 cgcgtgccgc accaaaacca acgtttgctt ctactttacc agttccggca ttacttgtca
     1081 taatgatgat cgtatcttta aagctgaccg ttcttccttg tgcatctgtc aatcgaccat
     1141 catctaagat ttgcaagaac atatgcaaaa catctggatg agctttttct acttcatcta
     1201 acaagacaag actgtaagga tttctccgta ctttttctgt taattgacca gcttcttcgt
     1261 atccgacata acctggagga gaaccaatca atttagagac actatgtttt tccatatatt
     1321 cagacatatc gaaacgaatc attgaatctt gtgaaccgaa tagttcgtag gcaagttgtt
     1381 tcgcaagctc tgttttacct acaccagttg gtccaacaaa taagaaggag ccgattggac
     1441 gattctgttt gtttaatcca acacggtttc ggcgaatcgc tttcgctact cgatctacag
     1501 cgttatcttg tccgatgaca tgagctttca agtcatctgc taagtttttc agttgtgtct
     1561 gttctttttc ttttaattca cccactggaa tacctgtccg ttgttcaacg attttttcca
     1621 tatctttttc ggtaatcaca ggtgtttcct cttcagtgag ctgacgttct ttcatttttt
     1681 gaagtttatt gatctgatcg cgataataag ctgctttttc gaaatcttct tctcgagaag
     1741 ctaaaacttt ttgttcttct gcttcttgca gttttttctc gattgtttta ggatcaacga
     1801 tttggatcgt caagtttttc ttagaacctg tttcatctag caaatcgatg gctttatccg
     1861 gcaagaagcg atcttggacg taacgattgg ataaagttgc ggctgcttcg atggcctcat
     1921 cagtatattt tacatgatga tagtcttcat aacgtttttg caaacctttc aaaatactga
     1981 tcgtttcttc gacagttggt tcatctaccc gtacaggttg catacgacgt tctaaagcag
     2041 cgtctttttc gatgatgcgg tactcattta aggtagtagc accaaccatt tgcaattctc
     2101 ctcgagctaa agctggtttt aaaatattac cagcatccat atttccatca ccagcagaac
     2161 cagcaccgac gatttcgtga acttcatcta tgaataggat cacattttct gcttgacgga
     2221 tctcttcaat caatttttgc atacgttctt caaattgtcc tcgtatacca gtaccttgga
     2281 ctaaagatac gacatctaaa cgaatgactt ctttgtccat taatttttgc ggtacatcac
     2341 catctacgat tttttgtgct aaaccttcaa caactgctgt tttaccaaca cctggttctc
     2401 caatcaagac aggattgttc ttggttcggc gatttaaaat ttcaatgact cgtttgattt
     2461 cgtcatcgcg tccgatcaca ggatcgatat caccttgtcg tgcttgttct gtgatattga
     2521 ttccgtattc tcccaataaa ccttctgtat tgccttgagg tggctgacca ccattgaagt
     2581 tgttgtttcc gccaaattgt gttggtggtg tttgctcata atttccttgc tgttgcattt
     2641 ggcgagacat ggaacggaaa agatcgtcca aactgccaaa tccaaatgga tcattttggg
     2701 ccatattagt cagacctcca ttttgttgat ttttgatttt ttgataacag ctctgacagt
     2761 aatcgagttg tgtccgctga ccattgacag tggcatacaa gtgaatcgtt gcctcattct
     2821 tgccgcagtt ttgacaaagc atgaatcatt cacgtccttt cacaaaaatg ggtacatcca
     2881 tattttacat ctcgtttttc atgaggtaaa agaataaccc ctagtttttt aagagattta
     2941 tgaggtagaa aaggtgaaat ctctattgac cattcctgac ctttgactct tattatacta
     3001 aaaataaaaa gggaagcaag taattcgttt catatgaaaa caaagagggt caaaggaaag
     3061 aatgataaac gtcaggagaa gagaaagcga caagaaaaag gaaataaaca cgtttgttat
     3121 aaaatgtgaa gaaaaggatg aaaaagaatc cggaataatt tgaatacttt tatctgaaat
     3181 tgaatgaaaa ctgttgtatc taacgaaaaa aaatgttaat ctttacagat gaaagggtgg
     3241 catttgttag gattctctga attactaaaa aagaaaaccc tcacataaaa acaacactca
     3301 cttaaaggag accgattaat atggaaaaga aagaatttca cgtagtagca gaaactggga
     3361 tccacgctcg tccagctaca ctattagtac aaacagcaag caaatttaac tctgatgtaa
     3421 acttagagta taaaggtaaa tcagtaaact tgaaatcaat catgggcgtt atgtctttag
     3481 gtgtaggtca aggttctgat gttactatta ctgcagaagg tgctgacgaa gctgatgcaa
     3541 tggcagcaat cgttgaaaca atgaagaaag aaggcttatc tgaataatgg ttgaaatgct
     3601 aaaagggatt gccgctagtg acggtgtagc cgttgcaaaa gcttacctgc tagttcaacc
     3661 ggatttaaca ttcagtaagg caacagtaga agatactgcg gctgaagaag ctcgtttgga
     3721 tgctgcatta gctaaatcta ctgaagaatt gcagcaaatt cgtgaaaaag cagcgcaaag
     3781 cttaggtgaa gcagaagcgc aagtatttga tgcccatttg atggttcttt cagatccaga
     3841 aatggtaggt caaatcaagc aaaatattaa agacaacagc gtaaatgctg aatcagcgct
     3901 aaaagaagta acggatatgt atatcggtat gttcgaagca atggaagata atgcttatat
     3961 gcaagagcgt gctgcagata ttcgtgacgt tgctaaacgt attttagcgc acttattagg
     4021 cgttacttta ccaaatccat caatgatcaa cgaagaagtg gtcgttgttg cacatgactt
     4081 gactccaagt gatacagcac aattagatcg caattttgta aaagcgtttg taacagacat
     4141 tggtggacgt acttcacatt ctgctatcat ggcacgttct ttagaaattc cagcaatcgt
     4201 tgggacaaaa gaaattactg ctaaagtcaa agaaggcgat attttggctg tcaacgggat
     4261 cgaaggcgat gtaatcatcg atcctacaga tgaacaaaaa gctgaatttg aaaaagcagg
     4321 tgcagattac gcagcacaaa aagcagaatg ggaaaaatta aaaaatgcag aaactgtcac
     4381 agctgacggc aagcattttg aattagctgc aaacatcgga acaccaaaag atctagttgg
     4441 tgtccataat aatggtggag aagcagttgg tctgtaccgt actgaattcc tgtatatgga
     4501 ttctccagac ttcccaacag aagatgacca atatgaagct tacaaagctg tattagaagg
     4561 tatggaagga aaacctgtag ttgttcgtac aatggacatc ggtggagata aagaactacc
     4621 atatctgcaa ttacctcatg aaatgaatcc attcttagga tatcgtgcat tgcgtatcag
     4681 tttatctgaa caaggcgacg aaatgttccg tacacaaatg cgtgcattgc ttcgtgcatc
     4741 tgttcatggt aatttacgta tcatgttccc tatggtagct acactcaaag aattccgcgc
     4801 agcaaaagcg atctttgaag aagaaaaaca aaaattgata agtgaaggca aggaagtatc
     4861 tgatacgatc caagtcggca tcatgatcga gattccagca gcagcagtct tagcagacaa
     4921 gtttgcaaaa gaagtagatt tcttctctgt aggtacgaac gatttgattc aatacacaat
     4981 ggcagcagac cgtatgaacg aacgcgtttc ttacttgtac caaccatata acccatcaat
     5041 tttacgtttg atcaaaaatg taattgatgc agcacacgct gaaggaaaat gggcaggtat
     5101 gtgtggagaa atggctggag atcaaacagc tgttccattg ctagtaggta tgggtctaga
     5161 tgagttctca atgagtgcga catctattct taaaacgcgt agtttaatga aacgcttgga
     5221 cacagcgaaa atggctgaac ttgctgatcg tgcattgaaa gaatgcgata tgatggaaga
     5281 agtcgttgaa cttgttcatg aatatgtaaa ataaacaacg tatcaaaagc tggtgtggct
     5341 tgtaaaagag ctacgccagt ttttttgttc ttttttaaga aaacgttttt gtcagtaaga
     5401 atagacaaaa atagggctta agtttgagtg acagtttgta ggctatgtta tactatataa
     5461 gtgaaggggg acgtaaggtg aaggtattat tatattttga aagtgaaaag atgcttgcaa
     5521 aatctggaat cgggcgtgcg cttgatcatc aaaaacgtgc tctgacggaa gtcggtatct
     5581 cttatacact agatgaaaaa gaagattatg acatcctgca catcaataca tacggaatca
     5641 atagtcataa tatggtcaat aaagccagaa gagaaggaaa aaaagttgtc tatcatgcgc
     5701 attctacaga ggaagatttc cgaaattcct ttatcagatc gaaccagctt tctcctatcg
     5761 taaaaaaata tctggtagga ttgtaccaaa aagcggacta tctgatcacg ccaacacctt
     5821 attcaaaaca gttgttggaa agctatggta tccgtgtgcc gattcaagct atttccaatg
     5881 ggatcgactt agagaaatat caccctgatc caatgaaaga acaaaagttc agagaatatt
     5941 tcaaactgtc tccggatcaa aaagtaatca tttgtgtcgg tttgtttttc gaacgtaaag
     6001 gaatcattga tttcgtagaa attgctaaga agatgcccga atacacattt atttggtttg
     6061 gtcatgttcc catgtactcg attcctcgga acatccggaa aattgtaaaa gaagatcatc
     6121 ctgaaaatgt cctgtttcct ggttacattc gtggagagat catagaaggt gcgtattcag
     6181 gtgcagatct attctttttc ccttcttatg aagagacaga agggatcgtt gttttagaag
     6241 ctttagcaag caaacaaaac gtacttgttc gagatattcc agtgtataat ggctggttag
     6301 aagatagcaa gaactgctat atggggaaga caaacgaaga atttatccga ctgatccaac
     6361 agattaccaa tcaagaacta ccgagtacga cacaagccgg ttatcaaaca gcaaaagagc
     6421 gaagtatcaa taaaatcggt gaagaactta aaaatgtata cgaaagtgta ctgaatgaaa
     6481 aagtctctag caaattaaaa aaagtaaatc aggtcaagga ctagtttgga ggcgaaaaac
     6541 atgaaaatcg gcttttttac cgatacctac tttccgcaag tcagcggagt tgctacatcg
     6601 atcaaaacat taaaacaaga attagaaaga catgggcatg aagtctatat ttttacaacg
     6661 accgatccta atgcggataa gttggaaaaa gatgtgattc gaatgccaag tgtaccattt
     6721 atttctttca aggatcgaag agttgttgtt agaggcatgt ggtatgctta tttggttgcc
     6781 aaagaactag aactagatct tattcatacc catacagaat tcggagcagg gatacttggg
     6841 aagatggtag ctaagaagtt gaaaatacct ctgatccata cttaccatac gatgtatgaa
     6901 gactaccttc attatatcgg taaaggaaaa gtgatcaggc aatcccatgt aaaatacttg
     6961 tctagattat ttgcaaatca tacgacaggt gtagtttgtc caagtgaacg tgttattgat
     7021 acattacgca gctatggtgt aatagcaccg cttcgcgtga ttcctactgg tatcgacatt
     7081 gaaaaattca aacgttctga tatcactcag caggatatca gcaaattaag ggagtcacta
     7141 ggtcttcagg aaaaccattt gatgattctt tctttaagca ggatttctta tgagaaaaat
     7201 atccaagcct tgattaatgg gtttcctcag gtcatctccg cttatcctaa cgccagatta
     7261 gttatcgtag gaaaaggacc ttatgtagaa gagttagagg aattgatctc agaactgcag
     7321 ttagaagaat ttgtgcagtt cacaggagaa gtggacaaca atgatgtagc actttattat
     7381 aaagcggcag attattttgt cagtgcttct acttcagaaa cacaagggct tacttataca
     7441 gaagcgatgg cagccggaac accttgtgtc gtggaaggaa atgcttattt gaaccgattg
     7501 ttcgatcatc cttcattagg gaaaactttt gaaaccgacg aagactttgc accagcactt
     7561 attgattata tttccagtga agttcctaaa gatccaaaag ttttagaagc taaactttat
     7621 gatatttcat ctacacattt cggcgaagct atgatcgaat tttatcaaga catggttgtt
     7681 tatcatgaag aagagatgcg aagaaaagaa gaagaagact cgattgaacg aatcaaaata
     7741 aaattaaatt catttaaaag ataaaattca aacatttttt atgaaaaaat ccttgtttct
     7801 agcgtatact agcatagaag caaggatttt tgatttttag gaggtcgaaa aaatgaaact
     7861 tggttttatc ggaacaggcg tcatgggaag tgctgtcgca cgccatctac tagaagcagg
     7921 acacgaagta gctgtctata atcggacaaa agcaaaagct gatcctttag taacagaagg
     7981 agctatctgg gcagatacac caaaagcggt agcagaacaa agtaatattc tctttacaat
     8041 ggtaggttac cccaaagatg tagaagaaat ctactatgga cagtcgggga ttttttctgc
     8101 agatatctca ggacatattt tagttgattt gactacaagt acaccaagcc tggcagaaaa
     8161 aattgcaaaa acagcaaaag aaaaaggcgc agatgcgtta gatgcaccgg tatccggtgg
     8221 tgacttagga gcaaagaatg gtacactgac gatcatggtc ggtggagaag aagccgttta
     8281 tgatcaagta ctgcctttat tcaaagaatt tggtacgaca tttacattgc atggttctgc
     8341 tgggaaaggt cagcatacaa aaatggctaa tcagatcatg attgcaggta cgatgacagg
     8401 aatgacggaa atgcttgtct atgcacaaaa aaacggactt gatttaaaga aagtgattga
     8461 gacgctttca ggtggaagtg cagctaactg gtctttaagc aattattctc ctcgtatttt
     8521 aaaagaagat tacacacctg gtttttttgt caaacatttt atcaaagatt taaagattgc
     8581 attagaagaa gcagaaaaaa tggatcttgt attgccagca actacccaag cattgaaact
     8641 atatgaagag ttggcagata aaggttttga gaatgacgga acccaagctt tgatcaaact
     8701 ttggtggcct gaaggaaaaa taccagaaaa aaaatcataa aaaacgagtt tttttagaga
     8761 aaatcaatgc aagtaggcta tgcttttgat acaatataac agaaataaga aaaggaggac
     8821 ctccgatgac acattcacaa ttagtcgcaa tcataaaacg actggaggct atggtcgagt
     8881 cagcagataa tgaactgcaa gttcgccgtt ttgaaaaaga aggcgtagag aagtgtacag
     8941 taacttatga taaagcgacc gatacatttg aattaactga aacagatact catcagcaat
     9001 acgaatttga taatatcgat atcgtagcga tggaaatcta tgacttaatc caataaattt
     9061 taggataatt gacatttgaa gttgaagatg cctaaaagaa gccgagaagc aactaattta
     9121 gttgcttttc agcttctttt ttgtttaaca gttcaatttc aaactgttct ttttctaatt
     9181 ttttgcggta actgatcaag atttgtacag gatcgatttc ttcttttgac aattgacgtt
     9241 tcaaagataa atgagcttct ttcttttcat aatagaagtc aggagaaacg tcaccagagg
     9301 taatcattgt ttctgcaagt tgaccataac aaacgaggta gtgctttttc tgactctcat
     9361 ctgctgctaa tggcatgctg aaggtgagcg atggtgttct taaaaactgc ttttgttcct
     9421 ggatccgtct aataaaataa gtaactaatt cgatatcttt ttgaagcata ccggcttttt
     9481 cgaaattcaa ctgatagatg aaatgttgtt gtcttttttt caagtaagtc agtagctttt
     9541 tatttcggcc ttgaaacagc ccttggagtt cctttttttt ctgttcaaaa gtcatttcct
     9601 gtaggtcagg aagctggact cgcaaagcta acaaactaat ttcagacaac caaggcattt
     9661 gataagtttc ttccattata cgcaaaatag acggcatttt tttatagaga cgaaaaggcc
     9721 cgtatgttcg agccgtagga gtatctgtta atacaaatcc cttattggta atatgtacat
     9781 aattataatt actgaaataa ttcatttgtc ggttataaaa aggacggtaa tgttggatca
     9841 attgacactc gagcaataaa gcatctaatt ccgtgtctac tacgacgaaa tcaaaatctg
     9901 ttatatgatg aatcatacgt aatacttttt ttgaatgctg tttattctga tgaaaatagc
     9961 tgcttactct atttttgagc cgttttgctt taccaacata gatgacctta ccatctcggt
    10021 ctttcataag atagacaccc ggcagcatgg ggagagtttt cattttttct tttaacatag
    10081 gatctccttt tacgctttga ataaaaagac tataacaaat tttgaaataa tggacaagtt
    10141 ttagggaatt ctatagttct gttc
//
LOCUS       DAZUMI010000106         9870 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000106 DAZUMI010000000
VERSION     DAZUMI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.109"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACG81C_002284"
     CDS             complement(18..263)
                     /locus_tag="ACG81C_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5065426.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(378..956)
                     /locus_tag="ACG81C_002285"
     CDS             complement(378..956)
                     /locus_tag="ACG81C_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5065427.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1033..1563)
                     /locus_tag="ACG81C_002286"
     CDS             complement(1033..1563)
                     /locus_tag="ACG81C_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5065428.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACG81C_002287"
     CDS             complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACG81C_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5065429.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2420..2815)
                     /locus_tag="ACG81C_002288"
     CDS             complement(2420..2815)
                     /locus_tag="ACG81C_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5065430.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACG81C_002289"
     CDS             complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACG81C_002289"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5065431.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACG81C_002290"
     CDS             complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACG81C_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5065432.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4843..5082)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACG81C_002291"
     CDS             complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACG81C_002291"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5065433.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6709..7866)
                     /locus_tag="ACG81C_002292"
     CDS             complement(6709..7866)
                     /locus_tag="ACG81C_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5065434.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACG81C_002293"
     CDS             complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACG81C_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5065435.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9333..9860)
                     /locus_tag="ACG81C_002294"
     CDS             complement(9333..9860)
                     /locus_tag="ACG81C_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5065436.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2507 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
      361 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
      421 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
      481 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
      541 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaatcgc taaaggttct
      601 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
      661 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
      721 tgattgctta taatgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
      781 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
      841 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
      901 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
      961 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
     1021 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
     1081 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
     1141 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
     1201 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
     1261 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
     1321 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
     1381 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
     1441 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
     1501 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
     1561 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
     1621 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
     1681 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
     1741 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
     1801 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
     1861 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
     1921 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
     1981 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
     2041 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
     2101 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
     2161 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
     2221 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
     2281 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
     2341 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
     2401 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
     2461 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
     2521 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
     2581 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
     2641 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
     2701 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
     2761 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
     2821 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
     2881 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
     2941 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
     3001 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
     3061 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
     3121 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
     3181 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
     3241 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
     3301 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
     3361 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
     3421 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
     3481 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
     3541 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
     3601 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
     3661 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
     3721 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
     3781 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
     3841 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
     3901 agcaggctcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
     3961 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
     4021 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
     4081 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
     4141 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
     4201 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
     4261 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
     4321 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
     4381 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
     4441 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
     4501 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
     4561 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
     4621 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
     4681 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
     4741 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
     4801 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
     4861 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
     4921 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
     4981 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
     5041 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
     5101 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
     5161 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
     5221 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
     5281 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
     5341 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
     5401 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
     5461 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
     5521 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
     5581 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
     5641 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
     5701 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
     5761 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
     5821 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
     5881 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
     5941 acagcaaaat tataagttgg cataccatcg cgtttttgta tgacaaagtc gccaccgata
     6001 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
     6061 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
     6121 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
     6181 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
     6241 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
     6301 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
     6361 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
     6421 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
     6481 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
     6541 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
     6601 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
     6661 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
     6721 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
     6781 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
     6841 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
     6901 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
     6961 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
     7021 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
     7081 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
     7141 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
     7201 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
     7261 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
     7321 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
     7381 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
     7441 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
     7501 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
     7561 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
     7621 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
     7681 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
     7741 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
     7801 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
     7861 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
     7921 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
     7981 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
     8041 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
     8101 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
     8161 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
     8221 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
     8281 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
     8341 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
     8401 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
     8461 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
     8521 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
     8581 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
     8641 ggctatccca tttgttttag cgattttgag tagttctgca gtggtttctc ttacttggct
     8701 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
     8761 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
     8821 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
     8881 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
     8941 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
     9001 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
     9061 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
     9121 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
     9181 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
     9241 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
     9301 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
     9361 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
     9421 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
     9481 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
     9541 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
     9601 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
     9661 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
     9721 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
     9781 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
     9841 tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DAZUMI010000107         9818 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000107 DAZUMI010000000
VERSION     DAZUMI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.110"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..786
                     /locus_tag="ACG81C_002295"
                     /pseudo
     CDS             278..786
                     /locus_tag="ACG81C_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            879..2041
                     /locus_tag="ACG81C_002296"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="ACG81C_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5065437.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..3301
                     /locus_tag="ACG81C_002297"
     CDS             2321..3301
                     /locus_tag="ACG81C_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065438.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3423..4841
                     /locus_tag="ACG81C_002298"
     CDS             3423..4841
                     /locus_tag="ACG81C_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5065439.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4912..6381
                     /locus_tag="ACG81C_002299"
     CDS             4912..6381
                     /locus_tag="ACG81C_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5065440.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6391..6858
                     /locus_tag="ACG81C_002300"
     CDS             6391..6858
                     /locus_tag="ACG81C_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5065441.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /gene="scrK"
                     /locus_tag="ACG81C_002301"
     CDS             6894..7766
                     /gene="scrK"
                     /locus_tag="ACG81C_002301"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5065442.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7847..>9818
                     /locus_tag="ACG81C_002302"
     CDS             7847..>9818
                     /locus_tag="ACG81C_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5065443.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEI"
BASE COUNT         3397 a         1427 c         1965 g         3029 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg
     3241 cgaaaaggag acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata
     3301 agttaaatcg gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac
     3361 atatgaaacg ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga
     3421 aaatggagaa attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg
     3481 tttgggtcgg agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac
     3541 gtgatactag aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga
     3601 aagattttgt tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga
     3661 gagaccagtt tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt
     3721 atacagggta taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg
     3781 ctgagagtga tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac
     3841 aagaagggta tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag
     3901 aagaatattt gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac
     3961 gtttagtgga atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg
     4021 ctccgggatt gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt
     4081 atcttgtcta ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt
     4141 tggaaggtcc ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag
     4201 gtagaacagc ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc
     4261 aagaaaatga taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc
     4321 aaaaagagga tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa
     4381 atttacatga tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc
     4441 ttgtcaaaaa tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc
     4501 ttagtgactt ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc
     4561 gcttcaattt agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc
     4621 aatatatgga taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac
     4681 taaaagtgat tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg
     4741 ctcgtgtaca tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga
     4801 ctgcaataaa taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc
     4861 aggtctgaaa atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac
     4921 aaaaaaattg ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct
     4981 acacactgca ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa
     5041 atgcttgagg aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta
     5101 attttcggaa caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct
     5161 aaggaagtat ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt
     5221 caacgagctt ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg
     5281 gcagcaatgg ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat
     5341 ggctcattcg ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt
     5401 gcaaccacat ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc
     5461 ggaaacccta ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat
     5521 cggaatgatt tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc
     5581 attccagcag ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta
     5641 gcaaaagttg agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc
     5701 ccaactgtga caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg
     5761 aattttatcg gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc
     5821 tttggtgctt ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat
     5881 gctattcaag gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct
     5941 atttggtcag tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt
     6001 aagaaaaaat ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt
     6061 attactgaac cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc
     6121 tcgtttattg gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt
     6181 ggacaaggat tgactgtatt gccaggtctg actattgcta atccagttct tccttatata
     6241 tttggtaatc tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat
     6301 aaagtgttta tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat taaatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat ctttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat
     7861 gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca
     7921 agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt
     7981 attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa
     8041 tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc
     8101 cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat
     8161 aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct
     8221 tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc
     8281 aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg
     8341 ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga
     8401 tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca
     8461 aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt
     8521 agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc
     8581 tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt
     8641 tgaatttctt aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact
     8701 ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat
     8761 caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg
     8821 ggacaaacta gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga
     9001 gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat
     9061 atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg cggatatcag
     9121 tctatttata atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa
     9181 gttcagaaaa tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag
     9241 tagtttagaa gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt
     9301 aaagaagttc tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat
     9361 aacagcttct gcgatcgcaa gggaaacaaa taagttgctg aatacatatg tttatgaggc
     9421 tttcgatatg ccaattgaag gtgagactag tgacttaatt aacctaatca acaatttttg
     9481 taaaagtata gatacgacga atggactcat tctgctagtt gatatgggat cattggaaca
     9541 aatgtatgaa aaaattgaat ccaatgtaat aggtgatttg gtaattttaa acaatgtttc
     9601 tacagcttta gcgattgaat gtgggattca agtttgccaa aaaaagccaa ttagtcattt
     9661 ttaccagatg gattttgatt cgtttcaagt taaagttcag tattataaag gattatcaca
     9721 aaagaaaaat gtaatagtat cttgtctctc tggtgagggg atttctgaaa aagttaagga
     9781 tattttaaaa cggtatctcg ataatcaagt ggagattt
//
LOCUS       DAZUMI010000108         9739 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000108 DAZUMI010000000
VERSION     DAZUMI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.111"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG81C_002303"
     CDS             33..656
                     /locus_tag="ACG81C_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065444.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG81C_002304"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG81C_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5065445.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG81C_002305"
     CDS             2152..3033
                     /locus_tag="ACG81C_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065446.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG81C_002306"
     CDS             3036..3806
                     /locus_tag="ACG81C_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065447.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG81C_002307"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG81C_002307"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5065448.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG81C_002308"
     CDS             5117..6280
                     /locus_tag="ACG81C_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5065449.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG81C_002309"
     CDS             6397..6807
                     /locus_tag="ACG81C_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5065450.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG81C_002310"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG81C_002310"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5065451.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG81C_002311"
     CDS             9380..9664
                     /locus_tag="ACG81C_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065452.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3383 a         1649 c         2090 g         2617 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaat
//
LOCUS       DAZUMI010000109         9667 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000109 DAZUMI010000000
VERSION     DAZUMI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.112"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACG81C_002312"
     CDS             complement(<1..632)
                     /locus_tag="ACG81C_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5065453.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACG81C_002313"
     CDS             975..1391
                     /locus_tag="ACG81C_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065454.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACG81C_002314"
     CDS             1388..1858
                     /locus_tag="ACG81C_002314"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5065455.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACG81C_002315"
     CDS             1872..2645
                     /locus_tag="ACG81C_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5065456.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACG81C_002316"
     CDS             2659..3471
                     /locus_tag="ACG81C_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5065457.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACG81C_002317"
     CDS             3525..4490
                     /locus_tag="ACG81C_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5065458.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACG81C_002318"
     CDS             4606..7329
                     /locus_tag="ACG81C_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5065459.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACG81C_002319"
     CDS             7342..7974
                     /locus_tag="ACG81C_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5065460.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9667
                     /locus_tag="ACG81C_002320"
     CDS             8873..>9667
                     /locus_tag="ACG81C_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065461.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1477 c         1884 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattta
//
LOCUS       DAZUMI010000110         9537 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000110 DAZUMI010000000
VERSION     DAZUMI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.113"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1172
                     /locus_tag="ACG81C_002321"
     CDS             201..1172
                     /locus_tag="ACG81C_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5065462.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1196..3388
                     /locus_tag="ACG81C_002322"
     CDS             1196..3388
                     /locus_tag="ACG81C_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS5065463.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            3405..3881
                     /gene="ybeY"
                     /locus_tag="ACG81C_002323"
     CDS             3405..3881
                     /gene="ybeY"
                     /locus_tag="ACG81C_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5065464.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            3865..4260
                     /locus_tag="ACG81C_002324"
     CDS             3865..4260
                     /locus_tag="ACG81C_002324"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5065465.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            4275..5174
                     /gene="era"
                     /locus_tag="ACG81C_002325"
     CDS             4275..5174
                     /gene="era"
                     /locus_tag="ACG81C_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5065466.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            5317..6129
                     /gene="recO"
                     /locus_tag="ACG81C_002326"
     CDS             5317..6129
                     /gene="recO"
                     /locus_tag="ACG81C_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5065467.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            6513..7430
                     /gene="glyQ"
                     /locus_tag="ACG81C_002327"
     CDS             6513..7430
                     /gene="glyQ"
                     /locus_tag="ACG81C_002327"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5065468.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            7432..9507
                     /gene="glyS"
                     /locus_tag="ACG81C_002328"
     CDS             7432..9507
                     /gene="glyS"
                     /locus_tag="ACG81C_002328"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5065469.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         3204 a         1731 c         2072 g         2530 t
ORIGIN      
        1 ctccactttc atataactta cattctcaga ttctttttat caaaacgaga cctttagcga
       61 atggaaacag ctatttttta acgtttcttg gtacgtatgg ttaaacgact cttttacaac
      121 cttttcttat ttgctatata gtttcttctg agtatggtaa aataggaaag aatgaatcga
      181 aaaaaagggg atgtctattt ttgacggaag agtctagttc tttagagatt agattgacag
      241 ctgctgatga tgtcagcatg cttttaggtg cacacgacaa gcatataaaa ctgatcgagg
      301 aaacaacgga aacaaccatc catacacgtg gagaaatcat tcagatcatt ggtgaacaat
      361 cccagatttc cttggctgcc tcagtcattc ggacattaca ggaattgatc aaacgaggaa
      421 tccacgtaag tacaccagat gtcgtgacgg cattgaaaat ggggcaaaaa ggtacattag
      481 attattttgt tgagatgtat gaggaagaga tcgtcaaaga ccgtaatgga aaacctatac
      541 gggtaaaaaa cagcggtcaa aagaaatacg ttgaatcggt agccaaacat gacatcgtat
      601 ttggtgtagg gccagccgga acagggaaaa cgtttcttgc cgtagtatta gcaattgctg
      661 ccctaaaaaa aggcgaagtg caaaaaatca ttttgacgcg gcccgctgta gaggcagggg
      721 aaaacctagg ctttctaccc ggggatctga aagaaaaagt ggatccttat ctgcggcctg
      781 tttatgatgc tttataccaa atttttggat tggatcatac gaaccgattg atggaacgag
      841 gcgtaatcga aatcgcacca ttagcttaca tgcgggggcg gacattggat gatgcattcg
      901 ttatcttgga cgaagcacaa aacacgacag tcgctcagat gaaaatgttc ttgacccgtc
      961 tcggttatca atcaaaaatg attgtcaatg gggatacaag tcagatagac ttgcctcgag
     1021 ggacgacaag tggtcttgtt catgcagaac gaacactaaa gcaaatcaaa aaaatcgatt
     1081 ttgttaattt tgaagcaagt gacgttgttc ggcaccctgt agttgccgaa atcatcaaag
     1141 cttatgaaaa ggcggatctt catcaagaat aagctttgct taggaggata agcacatgtt
     1201 gaataaaaca ctgcagaaac tacaagaaaa actcggaaag gcagcattgc ctttccttct
     1261 gttcttgttt tttatcattg tctgcggcgt tacatttagt agtgtcaaac aaaaggccaa
     1321 tgatttcaga gaaggccaag tagcagaaga gagtatccgt gcgaataaaa cgatcgagaa
     1381 cacggaagaa accgaacaaa gaagaaaact ggcagcagaa gcagtgactc cagaatatac
     1441 ttatcaaaaa gatctggcag atgaccagaa caatcggatc aagcaattat tcgaattgat
     1501 cgataagacg aatgatacca tcaacaaatc ttataatgac aaggtagata aggcgaagga
     1561 agatgaaaat atcccgaaac ctactacaga agagcggatt gctacactga agaaaaattt
     1621 tgaaaacgtg aatgcagaaa acgttgcgtt ttaccaaaaa ctgccgaaca atttttattc
     1681 gacgatattt gaaatgactg aaacggaaat tcacacggtt agggatgaaa gtttgaagct
     1741 cattgacgaa cagatgagta aacaagttcg tgaaagcgaa ttggaagctt ttaaacaaga
     1801 agcagaagat cagattcagt atttgaatgt cacaacagag cagcaacaaa tgattcgcta
     1861 cttagtggat caaggcattg tagtcaatga tgtattgaat gagaaaaaga cagaagaatt
     1921 aaaacaatct gcacgagaag ctgtacaacc agtgatgatc ttccaaggag aaatcattgt
     1981 tcgcgaagga aatcaaattg atgcttcagc gatgaaaaaa ttggaattac taggattgac
     2041 gaatcaaacg acttcgattt tcccactcat tgccatggtt ttagctgttt tgcttcaaat
     2101 cggcgtactg atttataatt cacttcaatt ccaaaatgca ggagaaagaa caaaatacgt
     2161 gcttttctat gtgacagcta tgtcagtcag catagtgctg atgaaatttt tccaattatt
     2221 ccaaacggaa caagctactt atatcccgct gttttatccc gctgcttttg caccacttgt
     2281 tttaagcttt ttcttgaacc gaagagcagg gatcatggct ggtattttcc aagcagtatc
     2341 tgcacttttc attttttatg attcgattgg cacgaacttt ctaacgatta tcttgatgtc
     2401 ttatgttttc tctgggctca tggcaacgat tgtcagacgc aaacggatct cagaacaagg
     2461 attatctgct gccatgtggg tcattatttt tcctgtgatg atggatgtca ttttgatcgt
     2521 ctatcaagga atgagctttg gtgatgcaac gacttggtta atgcttgtct gcgggttcac
     2581 tggtgcactc ttctcattct tgctgacgat cggtctacac ccatatattg aattgatggt
     2641 caatgatgac agtgtaattg tattaaatga actcagcaat ccaaatcatc ctttattgaa
     2701 acagttgctg gaagaagcgc caggtactta tcatcatagt atgatggtag ctaatctaag
     2761 tgctaacgct gtagcagaaa tcggcggacg ttctttgcta actcgagtag cttgctatta
     2821 tcatgatatc ggcaagatca aacatgctag ctttttcgtt gagaacttgc cttcaggagc
     2881 ggaaaacccg cacaatttct tattgccaga agacagtaaa catattatct ttggacatgt
     2941 gacagatgga gccaaaatat tggaagatta taatatgccg caaatggtga tcgatatttg
     3001 caaacagcat catggaacca cgctaatgcg ttatttctac atcaaagcga aagaacgcaa
     3061 tccggaagta acagaagagc aattccgtta tcctggacca aaaccgcaaa gtcgtgaagc
     3121 agggatcgtc aatattgctg acagttgtga ggctgcagta cgggcaatgg atcatccgac
     3181 gagcgaaaaa atcgaaagtt ttgtccataa tcttatcgaa gagcgtattt ccgatggtca
     3241 attagacgat agtggactga ccttgaaaga aatccgaaaa gtggaaaaat cattgatcag
     3301 cggtttaagt agcaccttcc attctcggat caaatatcct aaaatgaaaa cagaagttga
     3361 gaagataaag gaagaacaag aagaatagaa agaagcgatg tataatggat attaccttta
     3421 tggatgaaac aaaccaagtc tcagaagaga aaatgaaaga aatcgatgac ttgctgcaat
     3481 ttgcagcatc ttatttaaag ctgccagagg aaacagaaat gtctgttact ttcatggaca
     3541 atgcggcaat ccaagtaatc aatcgtgatt accgtggaaa agatgcaccg acagacgtca
     3601 tcagttttgc tctggaagaa gaaggcgaag acgaaatccc agtgattttt gaagatggag
     3661 agaatccttt gcctcgtgta ctaggagaca tcatgatttc tattgaacgt gctgctgaac
     3721 aagcagaagc ttacggccat tcatatgatc gggaacttgg ctttttagcg gtacatggat
     3781 tcttgcatat caacgggtat gaccatatga caccagaaga tgagaaagaa atgtttggtt
     3841 tacagaaaga gatcttggat gcctatggac tcaaaagata aaaacgtaga gaaaaacaaa
     3901 catttcctga tgtctgtgga atttgctgtc caaggagtca aaaccgtttt tgaggaagaa
     3961 cgcaatatga ggaaacatgt tgcgttcggt atccttgctt tatttatggg cgtgattttc
     4021 cagcttgatc ggatagagtg gttatggctg cttttagctg tttttctcgt ttggatagta
     4081 gagatcatca atactgtttt tgaaaatgta gtggacatgt ttacggattt tcattttcat
     4141 ccgattggga aaaaaatcaa agatatggca gctggagcag ttttagtgac cgcttgtttt
     4201 gctgtcatca ttgggttgat tttatttgtc cctaaaatat atcatttgtt gtttaattaa
     4261 aaaggagaac gtatatgcca gaacataaat cagggtttgt cgcaatcgtc ggccgcccga
     4321 atgtaggaaa atccacatta ttgaaccgaa tcgtgggtca aaaaattgcc attatgagtg
     4381 acaaggcaca gacaactaga aataaaatcc aaggagtata tactacacca gaagctcagc
     4441 tgatttttat cgatacacca ggaatccata aaccaaagca tcgtctagga gattttatgg
     4501 tagaagcagc ttacagtgcg ttgaaagaag tggatgcagt cttatttatg atcagtgctg
     4561 atcaaaaacg cggccgggga gacgatttta ttattgaacg tctaaaaaac gtgcattctc
     4621 cagtttatct gattatcaat aaaatcgata agatccatcc agacgagttg ctgggaatca
     4681 tcgaagatta ttcgacgcag atgccttttg cacaagtgat tccgatctct gctacagaag
     4741 gaaacaatgt cgaacgctta atggatgtat tagtcagtga aatgccagag ggcccacaat
     4801 attttccaga tgatcaagtg actgatcatc ctgaatactt tatcgtttct gaattgatcc
     4861 gtgagaaagt cttgttttta acacgagacg aagtgcctca ttctgtcgca gtcgttgttg
     4921 attcgatgaa gcgaaacgag aatgataaaa tccatatcca ggccacgatc atcgtcgaaa
     4981 gagatagcca aaaaggaatc attatcggca aaggcggaaa aatgctgaag caagtaggaa
     5041 cgaaagcaag aaaggatatc gaaaatcttt taggagacaa agtatttcta gaactttggg
     5101 tcaaagtcca aaaagactgg cgagacaaaa aagtctatct gcaagacttt ggctaccgaa
     5161 aagacgaata ctgatattct gaacttctta taggttaagt atagaaataa actggataaa
     5221 aatccgcacg ttgagaaaaa cggcggaaga tcgaaacata aataatctgg atcggcagaa
     5281 tctgcttttc cagatttatt ttttaggagg gagaatatga gccagcttgc agaatcgaaa
     5341 ggcttgattt tatctagccg taattacaaa gaaaaagata aattagtcaa aatctttacg
     5401 gaatcttctg gcaaaatgat gttttatatc aaaggaatcc atcggcaaaa tcagccatta
     5461 gctccggctt tgctcccctt taccgaagca gtctatatcg gtaattttcg aagtgaaggt
     5521 ttgtcgtttt taagcagtgt caaagacgtc catgcgtttc ggaacatcca agaagatatc
     5581 tttttgagcg gttatggtac ctatttgcta aatctagtag atgcagcaat cgaagatcat
     5641 caatatgacc cgcatttatt ccaatttacg caacaagctc tgcagcggat ggatcaagga
     5701 gatgatgcag aaatcatcac gaatatcttt gaagttcaaa tactgcaacg cttcggaatc
     5761 gcgccaattt ggacgcactg tgtcgtatgc ggggaaacaa aaggaaaatt tgactattct
     5821 tccaaatctg gcggtgtgat atgcgagaaa cactggccaa tggacgaaca ccgttatcat
     5881 gcagatcctc gcgctgttta tttcgttcga atgttttcgg ctatttctta tgataagatc
     5941 agtggaatcc acttgaaaga agaaacgaag caggcaatcc gtgacctcat tgaccagttg
     6001 tatgatgaat atgtcggtat tcatctaaaa agcaaaaagt tcattgatca aatgaagtct
     6061 tgggcacacg tattaaagac gccagagaaa aatatgcctg aaaaagagat acctgaaaaa
     6121 gaagaataaa tgaaagatac aacaaaaaaa tcaaaactat ttgacaaata cagggacttc
     6181 cgtctattat taaccgtaga ttaaatagat cgttcactga gaggatctat tatataaaca
     6241 agaaaactgc aaaatgaata cgtacattga ccaagatcag taaaaaacac gtggtttagc
     6301 gagttcggga gagtggaagc cggacccaaa cgatttttga agaggcactt gcgagtacga
     6361 taaatgaagc tgccgaatct ttattcggaa gtagggtgga accgcgaaat tattcgtccc
     6421 tatgtctgta atcagacata gggacttttt gtcgttttct ttcctgtatt cttattgaca
     6481 gcaacgctta aaaattgaaa ggggcaaaac agatgagtaa aaaattgacc gtccaagata
     6541 tgattttaac attacaaaaa ttttggtcat caaacggctg catgctgatg caagcttatg
     6601 acacagaaaa aggagcaggt acgatgagcc catatacatt cttacgggca atcggaccgg
     6661 aaccatggaa tgctgcttat gtagagccat cccgccgacc agctgacggg cgttatggag
     6721 aaaacccaaa ccgtttgtat cagcatcatc aatttcaagt cgtaatgaaa ccttcacctg
     6781 aaaatatcca agagctttat ttagaaagct tgcgtctttt aggaattgat ccattagaac
     6841 acgatattcg ttttgttgaa gacaactggg agaacccatc gatggggtgt gccggtgtgg
     6901 gttgggaagt atggcttgat gggatggaga tcacacaatt cacgtacttc cagcaagtcg
     6961 gaggactacc ttgtaaacca gttacttctg agatcactta tggactagaa cgtcttgctt
     7021 catatattca agaagttgaa agtgtctatg atctggaatg ggctgatggt gtgaaatatg
     7081 gcgagatctt caaacaacca gaatatgaac attccaaata ttcttttgaa gtaagtgacc
     7141 aagacttgct actaagcaac tttgaccgtt ttgaaaaaga agctaaacgc tgtatcgatg
     7201 aaaatcttgt tcatccagcg tatgattata ttttgaaatg cagccataca ttcaatttat
     7261 tagatgctcg cggtgcagta tccgtaacag aacgagcagg ttatctttcc cgtatccgca
     7321 acatggctcg tgctgtagcc aaaatctttg tggcagaacg cgaaaaatta ggattccctt
     7381 tattgaataa aaacacagca aatccagaaa acatgaagga ggctgaataa gatggcaaaa
     7441 gacttattac ttgaaatcgg gctggaagaa atgcctgctc atgttgtcac accaagcatg
     7501 aaacaattag aagaaaaaac agcaaaattt ttagatgaaa accaattgac ttatgatacg
     7561 atcgaaacgt tttctacacc gcgccgctta gcaatcaaag taacaaacat tccagaacgt
     7621 caagcagaca gcgaagaaga agtcaaagga ccagccaaaa aaattgcttt ggacgcagat
     7681 ggaaactgga cgaaagcagc tgaaggattt gtccgcggac aaggtctgac gacagatgct
     7741 atcactttta gagaattaaa aggcgtggaa tatgtctatg taacgaaata cactcacggc
     7801 aaagaagcaa aagaagtatt gacaggttta gcagatgtca ttaaaagcct cactttccca
     7861 gtaacgatgc actgggcaga atacgatttt gaatatattc gccctattca ctggatcgtg
     7921 gcattgttag atgacgaaat aatcccgttt gagatcttgg atgtcaaaac agacagagct
     7981 tcaagaggtc atcgtttctt aggcgaagat gtgacattta cgcatgcaaa agaatacgaa
     8041 gaaaaattga atgaacaatt tgttattgct tcaccgcaaa aaagaaaaga aatgatcatc
     8101 acacaagcaa acgaattagc cgaaaaaaat cactggatca ttgatctaga tgaagattta
     8161 ctagaagaag tcaacaattt agtcgaatat ccaactgtct tttctggcga cttcgaagaa
     8221 aaatatctat ctgttcctga agaagtacta gtcacttcta tgaaagaaca tcaacgttat
     8281 ttcgaagtac ggaacgatca aggtatgttg cttcctcatt ttatttctgc tcgtaatggc
     8341 gatcgggtaa aattggaaaa tgtggtacga ggcaatcaaa aagtattgac ggctcgttta
     8401 gaagatgcgg aattctttta taatgaagac aagaaagctt caatcgacca atacgttgaa
     8461 aaattaaaaa ctgttacttt ccatgaaaaa atcggtagca tctatgagaa aatgcaacgg
     8521 gtacaagtca tcgctcgcat cataggcgaa caagtaggac tatctgagga agaattgatg
     8581 gatctgcaac gcgctgctga aatctataaa tttgatctag taaccaacat ggtaggagaa
     8641 ttccctgaac tacaagggat catgggagaa aaatatgctc tgctgaaagg cgagaaacca
     8701 gccgtagcaa cagcgatccg tgaacattac atgcctatat ccagtgaagg agatttacct
     8761 gagactacag taggttctgt tttagcgatt gctgataaat tagatagtat cttttctttc
     8821 tttgcagttg ggatgatccc aagtggctca aacgatccat atgctttgcg ccgtcaagca
     8881 tatggtgtcg ttcgaatcat cgaatcgaaa aaatgggcct ttcctttgtc tgttctgcaa
     8941 gaaacgatta gtgaagttat cagtaaagat acggatcgtt ttggcattgg cttgtcagcg
     9001 ggacaacaac aggtgatcga ctttatcaaa ggccgtctgc gccaattatt gacaacgaag
     9061 aatatccgtc atgatgtcat cgaagctgtt ttgaatgcag aacaaaaaga tctgacaaaa
     9121 gtatttgcag cagcacaatt gttcaagcag catcttgcag atgaagactt caaaccatcg
     9181 atggaagctt tgacacgtgt cgtgaactta gcgaaaaaag cggagcttga aaaacaatca
     9241 gaagtagacc ctgaactgtt cgaaaacgaa gcggagaaag aactgcacaa agcagtagag
     9301 gcaatgaaaa caagctttgc tgaccaaacg atcaacgaaa attatgatca gcttgtaagc
     9361 cttcgcccac tgatcgaaaa ttattttgat cagacgatgg tcatggcaga agaaccagca
     9421 gtacgtaaga accgattaac gcaattaagt caaattgctg ccatggcttt gtcactagca
     9481 agtttagatc aaatcatcac aaaataattt ctaatatgta aataaaccag agaacag
//
LOCUS       DAZUMI010000111         9443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000111 DAZUMI010000000
VERSION     DAZUMI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.114"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..205
                     /locus_tag="ACG81C_002329"
                     /pseudo
     CDS             59..205
                     /locus_tag="ACG81C_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="ACG81C_002330"
     CDS             282..611
                     /locus_tag="ACG81C_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5065470.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="ACG81C_002331"
     CDS             601..888
                     /locus_tag="ACG81C_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5065471.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1174..2031
                     /locus_tag="ACG81C_002332"
     CDS             1174..2031
                     /locus_tag="ACG81C_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5065472.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            2032..2580
                     /locus_tag="ACG81C_002333"
     CDS             2032..2580
                     /locus_tag="ACG81C_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065473.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2840..3301
                     /locus_tag="ACG81C_002334"
     CDS             2840..3301
                     /locus_tag="ACG81C_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS5065474.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            3345..3431
                     /locus_tag="ACG81C_002335"
                     /pseudo
     CDS             3345..3431
                     /locus_tag="ACG81C_002335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3397..4467)
                     /locus_tag="ACG81C_002336"
     CDS             complement(3397..4467)
                     /locus_tag="ACG81C_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5065475.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            5069..6913
                     /locus_tag="ACG81C_002337"
     CDS             5069..6913
                     /locus_tag="ACG81C_002337"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5065476.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6969..8435
                     /locus_tag="ACG81C_002338"
     CDS             6969..8435
                     /locus_tag="ACG81C_002338"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5065477.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8472..9314
                     /locus_tag="ACG81C_002339"
     CDS             8472..9314
                     /locus_tag="ACG81C_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5065478.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3246 a         1443 c         1906 g         2848 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataagg
     1081 tagtacatat atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca
     1141 aatgaatatt gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt
     1201 aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca
     1261 gttaatgaag gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat
     1321 gtgatgaact taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg
     1381 attgtttacg aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct
     1441 gtctataaag taatcaataa cctctatata cttccagcag acatgtcctt tgaaatgtat
     1501 gatgattgga tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa
     1561 aagctagaac cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt
     1621 tctgtatata gtaaatccgc gatgtacatt gcggattggg caatcgtcgt cttacagacc
     1681 caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa
     1741 gaatttgata caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac
     1801 gcagtagatc aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa
     1861 aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag
     1921 aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt
     1981 attttgaaag aattggaaga acacgagaat tggtatgtgg aggtggagta attggctgac
     2041 atcaaaggac ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca
     2101 gaagcaaaga aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac
     2161 caagttcaag aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa
     2221 aaagttggcg caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa
     2281 ctgtcgactt tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg
     2341 acaagagatg aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa
     2401 gaagataaac aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa
     2461 tatccaattg ttccagaatt agatgatcgt ggagaaataa taagaactac cagtcagata
     2521 aaacaagata ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagataa
     2581 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
     2641 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
     2701 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaataa
     2761 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
     2821 ctattaggag gagatgtaga tgaaaaagtt taaaaagaaa atatatttag gattggttgt
     2881 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
     2941 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
     3001 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
     3061 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
     3121 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
     3181 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
     3241 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgata
     3301 acgatcctaa ttggagtaaa caagctaatt taagagagat tagcatgcca tccgcttggg
     3361 aagaaaccaa gggttctaag gatgtgaagg tagcggtcat tgattcagga attgattatc
     3421 aacatgaaga tctttctgga aatgtagatg aaagtttggg atacgatttt ttttactttt
     3481 ttcaagaaaa taataattca gaatattctg actatattta tgcaacacta gtgactttag
     3541 gagaaacatc aatagccttg ctaaagtttt tgctttcgct aaagagagta ttttcgataa
     3601 tttgtttttt tctaaaatac ctcgttcctc aatatttggg atatggatat gtatagggat
     3661 tcttcgcata aaggtttcca gaaaatcctc tttgccttct gtagtcgcaa aaactaaacg
     3721 tacattggag gcgtgatgga tagtttcacc gactctagaa taagttccgc catccatata
     3781 aacaaatagt ttctcttgcc cttcaggagg caatctgtgt acttcgtcta aaaacagtac
     3841 tccaccgtct gcatcttgca acaatccatt tcgatcttca tctgcgccgg taaaagcacc
     3901 tttaacatag ccaaatagta tccccgacaa taattcagga ttattatggt attgcgcaca
     3961 attaagtact ttaaaaggag cattctcatg aataacgttc acttttttca taaactgata
     4021 agcagtatga acaaaaaaac tttttccaac tccagtatct ccagtgatca tcataggaag
     4081 ccctcttcca ggatagaaga cagcagtttt catttgttcc accgcatcgg ataagctcat
     4141 atcataccca atcatctttt gtagaatgga atctgtagaa ttgggctcca agtctgtttt
     4201 taattcagat atactttcgt atacatcttt taccggaatc aaaaagtgct cttcaaagcg
     4261 ctctttgatg gcaaataaca ctggacgagt attgatttta aatagaacgt tctttgcaac
     4321 taagtcattg gcaagtttac tggcatttgt tcttcccgtt cttaaaaatt gcgacaggtc
     4381 atttgtgtca aaaaagcact cttctaaatt actgatgcca atttcttcac attttgtgtt
     4441 tgcataagtc actatatcat ttttcatctt ataaacagtc ctttctaaac ttctatccct
     4501 taaataaata gcaaaaaaaa ggaagcaata tcgatatgct gtcctcttaa tttaataaag
     4561 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
     4621 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
     4681 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
     4741 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
     4801 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
     4861 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
     4921 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
     4981 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
     5041 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
     5101 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
     5161 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
     5221 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
     5281 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
     5341 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
     5401 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
     5461 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
     5521 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     5581 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     5641 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     5701 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     5761 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     5821 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     5881 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     5941 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     6001 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     6061 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     6121 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     6181 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     6241 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     6301 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     6361 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     6421 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     6481 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     6541 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     6601 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     6661 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     6721 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     6781 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     6841 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     6901 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     6961 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     7021 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     7081 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     7141 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     7201 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     7261 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     7321 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     7381 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     7441 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     7501 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     7561 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     7621 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     7681 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     7741 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     7801 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     7861 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     7921 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     7981 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     8041 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     8101 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     8161 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     8221 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     8281 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     8341 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     8401 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     8461 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     8521 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     8581 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     8641 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     8701 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     8761 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     8821 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     8881 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     8941 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     9001 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     9061 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     9121 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     9181 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     9241 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     9301 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     9361 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     9421 ttaacgtaag ataataaaaa atc
//
LOCUS       DAZUMI010000112         9410 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000112 DAZUMI010000000
VERSION     DAZUMI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.115"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      complement(21..87)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(129..776)
                     /locus_tag="ACG81C_002340"
     CDS             complement(129..776)
                     /locus_tag="ACG81C_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS5065479.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1055..1558)
                     /locus_tag="ACG81C_002341"
     CDS             complement(1055..1558)
                     /locus_tag="ACG81C_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5065480.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1681..2445)
                     /locus_tag="ACG81C_002342"
     CDS             complement(1681..2445)
                     /locus_tag="ACG81C_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5065481.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2552..2827
                     /locus_tag="ACG81C_002343"
     CDS             2552..2827
                     /locus_tag="ACG81C_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5065482.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3504..4454
                     /gene="yidC"
                     /locus_tag="ACG81C_002344"
     CDS             3504..4454
                     /gene="yidC"
                     /locus_tag="ACG81C_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5065483.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="ACG81C_002345"
     CDS             complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="ACG81C_002345"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5065484.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5604..6062)
                     /locus_tag="ACG81C_002346"
     CDS             complement(5604..6062)
                     /locus_tag="ACG81C_002346"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5065485.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6059..7522)
                     /locus_tag="ACG81C_002347"
     CDS             complement(6059..7522)
                     /locus_tag="ACG81C_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5065486.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="ACG81C_002348"
     CDS             complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="ACG81C_002348"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5065487.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8597..9346
                     /locus_tag="ACG81C_002349"
     CDS             8597..9346
                     /locus_tag="ACG81C_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5065488.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         2794 a         1994 c         1635 g         2987 t
ORIGIN      
        1 aagaaagggg ctatccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctggac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtgt tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgatctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgttcatt cttctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaaccg agaaaatcag ccagattttc
     1021 tcggaatata gtctattatt attggtttta cctattaaat cgaggattct ttcccaaagt
     1081 aacgttgcca tctcttacgc atacttgcag acatgggctg agccgcttct ttgactgcgc
     1141 agtatttgtc cacgaatgtc acaatatatc cttctttata cttaggcggt gcgatcgttg
     1201 caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatttttt
     1261 ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg tcaaatttcg
     1321 tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag
     1381 cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct
     1441 ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt
     1501 ctaaaagatc ttgtacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa
     1561 ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac
     1621 cagtgttcgg attattctag tcgctctaga taaaatagct aaagcttaat agttccttaa
     1681 tcataaagaa aaatgataaa gaacgatccc tgctgcgact ccgacattca atgattccgc
     1741 atttcctttc attgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac
     1801 tccctgacct tcgtttccca tgattatagc gaaatcctct gttttttcta ctttgtctaa
     1861 tgcatgtgct tcttcattta attctgtacc gtacaccgta aaccctttgc ctttcattac
     1921 agggattaca tctgccaaat ctttttgtaa cacaggcaaa tgataattgc ttccctgcat
     1981 gcttcgcagc acctttgtac tataaatatc tgccgttcct ttacctaata aaacaccagc
     2041 cataccaaac gcatctgccg tacggatcat cgttcccaca tttccaggat cctgtacatt
     2101 atctagtaaa agccagcctc ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac
     2161 tgctaagatc ccttgcggtg taggtagatc tgacatagcg gtcatgacct catctgaaac
     2221 tagaaaagta gaaatctctt tgtttacagc cagccagtct ccccattctt tcatacctct
     2281 ctgtgtcaca aaaagttctt ttatttctgc attagacgac gcagcttctt cgatcagatg
     2341 gaacccttcc aaaagatatt ctgccttttc ttctctgtat ttctttttat gtagtttttt
     2401 taattctttg attcttggat tttttgctga ttggatctct ttcacattcc tcacctcaac
     2461 tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg
     2521 atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg
     2581 acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc agttaggcgt
     2641 gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga
     2701 cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa gtggacgagt
     2761 gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat ttgatgtagt
     2821 gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg
     2881 gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt tgaacaccgt
     2941 cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat tttcaaaata
     3001 gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata aaacaccgtc
     3061 aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt gataaatagc
     3121 tctccatatt tttcaacagg ctggacttaa tgccgtagat gcaagctttt gaacaccgtc
     3181 gataaggttg tttttgaggc gctttgcctc aagtattaaa gacaggtagt aaaaaagctg
     3241 atctataact tgcaatgaat ttgcttaacc catattttcc agacagaaca aaaaagtttt
     3301 tagtttttta aataagaaag aaacacttgg aatcactctt ttctttctaa gtatttcttt
     3361 cttgattttt tgtctttttt caaaattttt cgctacgaaa aagaacgaat atttatatga
     3421 aatttctatg aagttgattg aattccatgc gcttttttag tatagttaag tttgtgtaaa
     3481 aattatagaa gaggaagtat tctatgaata aatggaaaaa ctggttattg ggatccggac
     3541 ttgtcagtat cctgctcttt ttatctggct gtgtgcggat ggattcaaac ggaaaccctg
     3601 acacttcagg aatcgtttat cgagtactcg tccatcctat gggccaagcg attacatatc
     3661 tagttgagaa tttcaattgg tcttacggct gggcagttat tgcaatgact gtgatcgttc
     3721 gtatcatcat cttgccacta ggtatcagtc aatcgaaaaa aacaatgatc caatctgaaa
     3781 aaatgcaagc gttaaaacca caagttgaag cagcacagca aaagctaaaa gctgcaacaa
     3841 cacgtgaaga acaaatggca gcacaagcag aaatgcagca agtatatcgc gaaaatggat
     3901 tgagtatgac cggcggaatc ggctgtctgc ctctattgat ccaaatgccg atcttctctg
     3961 ccttgtattt tacggcacgc tacacagaag gtatccggga atcatctttt tacggcatcg
     4021 atctaggctc accaagtatg gtgcttgtgg ccatcgcagg ggtcgcttat ctgcttcaag
     4081 gatatatttc aacaatcggt attcctgaag aacagaaaaa gacgatgcgt acgatgctga
     4141 tcgtctctcc tgctatgatc gtcttcatgt caatcagtgc gccagccggt gttacacttt
     4201 actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac gttatgatga
     4261 aaccacgttt gaaagcacaa gtggcagaag agttgaaaca aaacccgcca aaacaagtcg
     4321 tcactccaag aaaagacgtg acagaatcag caaagccagc taacaacaaa caagcaacaa
     4381 aacaaatcaa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca ggaaagcagc
     4441 agaagcggaa gtagatagta aataagagtg taataaaaat tcattaacag ttttagcaat
     4501 taaaaatatc gaccaagatt tttgaaaaag aaatctggtc gatattttta tttaagtgaa
     4561 tgtttaattt gaatcatcga ttgatcgcta tctaattgca gcagcatctt tttatgaaga
     4621 aacaactttt tcttatctat caacgtagac aggtgttgct gtttcttcta atagtgtact
     4681 taattcttta atattttgtt ttccttcttc taacatccaa ctactcgctt tttcttcgcc
     4741 ttcggtaaca aaaggtgcca ctcccttcgc ccatgttgct cgcccacata agacaccatt
     4801 gaatgtcgaa cctgattgtt ttgcaaaacg taatgtatct tgaaatagcg tagcattgac
     4861 tcccgcactt aaaaaaataa acggttgttc agtcactttc gattgttcat agaaatacgc
     4921 ggatgcctct gcttgactat aacaagtctc ttgcccatat ccttccacaa atctcatatt
     4981 cactggcact tctactttca atacatccac ttgataacgt tcttttgaaa attctcgcat
     5041 ggcttcaata actttatgtg gttttacttt tgcataggca agagaagacg gatcgccaat
     5101 agttgcatca taagaaagaa tctctaaaaa gaaaggtatg ccttctgctt cgcattctga
     5161 accaatccgt tctataacta tttgtttttg cacattaatc tctgctggtt catcaacgtc
     5221 atagtacaat aaaaatttac aagcatccgc accagcttct tttaaccttt tcaccgacca
     5281 aatattcaat aaatcaggta atcttcccgg tattttcgta tcatagccgg ttttttcata
     5341 agctagcagt aagccacatt ctttctctcg aagcgctgct gcaggcaggc cgtattctgg
     5401 atcaagtaaa atagctgagg catagggagt caggtattga gaaaccatct gtttaaattg
     5461 gatgatgtct tctgatgtag cttctttata ttggttgatc attttcttca aagcaccacg
     5521 ctggtcgatt gctaatgcgc taataattcc agcttcatcc gtcaatcgct tcaaacgttc
     5581 ctgtttttct atactcatct tcttcataga tcttcaccta tttctttagt taatattgtg
     5641 gctatttctg tttttgattt tgcttttctc aactgttctg taacctcttt tcgcatcaat
     5701 gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc ctgcttctga atctggaata
     5761 aataacgcaa aaataaaatc aactgactgt ctatctaaac tgttccattc aattcctttg
     5821 ttcagtgtgg cgataatcat actaggttcg ttgattgttt gagacttcgc atgagggata
     5881 gcaaaacctg acattattcc tgtagtccct tctttttctc tttctagaag tttttgatat
     5941 acttcttttt cgtcaagagc gatccctaaa cagaatgttt gctttgctaa aaacttaaat
     6001 gcttcttctt gactatccaa tggctgttgt aaaaaaatgt tctcttcatg aatgattgtc
     6061 atgataagtc tcctgctctt cctttttcga taactgtacg atttgctgct ttattatcca
     6121 tcttcttgtc tttatcagtt actcttttct tttggattaa tcctagtatc actccaccaa
     6181 cgatcgaacc aataagaatc gctgtaaccc attggatttt tcccgtcact acaggtaaca
     6241 ctaaaaaacc accatgtggt gctggaactt ggacgccaaa caaataagtt aagacagcag
     6301 caattgatga gcctaacatc atcgtgggta acactcggat cgggtctttc gcagcaaacg
     6361 gaatagctcc ttccgtaata tgagttgatc ctaaaataaa attaaccaaa cctgcattac
     6421 gatcattggt atcaaaatat ttaccaaaca ataaagtagc aaaaccagta atcaacggtg
     6481 gtgcaataca agctgctgac acaccagcca taaaagtcgt gtttccttgt gccaaaagag
     6541 ctgttcctgt tacatatgcc gccttattaa ccggtcctcc catatcaaac gcacacatac
     6601 agccaacaat cacgccaaga acaagtggat tcgaatgttc aaatccagct aaaaagctca
     6661 tcattccttc atttatagcc ttcattggac ctgccagcaa agacatcacg atgccaataa
     6721 atgctacacc aataactgga tataagaaga ttgccttcaa gccatctagt tgtttaggta
     6781 aaattgtcag tagcttttgc aaaaataaga taaaataacc tgctaagaac ccagctaaaa
     6841 tacctcctaa aaatccagta ccgccattca tggctacgat tccaccaaca aatccaacga
     6901 ctaaccctgg acgtttcgcg atcccttctg caatataagc tgctaaaact ggaaccatca
     6961 ttcccattgc tgcatcgcca actgctttta ccattgccgc tgtagcattg tattgtgcat
     7021 gatctggatc aaaagaatag attccccaaa cggcaaatga aatagcaatc aatactccgc
     7081 cagctaccac gaaaggcaac atatgagaga caccgttcat taaattttta tagatgctac
     7141 gaccaaaatt agcaccactc atcgcttctg gttctaaagt atcagcagct tcattatctg
     7201 ctaagacagt accttttcct gccaatgctt cgtcaatcag ccgttgtgca tccttgattc
     7261 ctgtactgac agaaacatca atgactcgtt ttcccgcaaa tctttctgaa tggacatctt
     7321 tatctgcggc aataatgaca gcatctgcct gtttgatttc ttcagccgtt aattcatttt
     7381 cgatcccaat ttgtccatgg gtctccactt tgatcttgat gccttttttc ctagctgctt
     7441 gttctaatgc ctcttgcgcc atgtaagtat gcgcaattcc tgtcggacaa ccggttgctg
     7501 caattagttg atattttccc atgtttcaaa cctctcttcc ttgattttga tttgtttact
     7561 taattcctcc acatccaaaa aatcagtcag tcctttacga aaagcggttg agctgcctgc
     7621 tgctatgctt ttacgtaacg tttcatcaag cggtcgtcga ttcatatatc ctgctaaaaa
     7681 agcgcctaac atggtatcac ctgcacatgc tgtattgaca acttttcctt ttggcgagtt
     7741 tcctcgaaac accctgtctt tcgtaaaaag aatcgctcct ttctcaccga gggataacaa
     7801 aacattttct gcacctaact ctactagtcg ttgtccataa acaaagtaat cttcatctgt
     7861 aaccattttt cgtccgaacc aagaagaaag ttcttcctcg ttcggtttca gcaaaaacgg
     7921 tcgatagggt aaacaatcca tggcttcttg atcactacta tcaataatca aatgaatccc
     7981 cttttcctga caaatccgac tgatttctat taaaaccttt ggagacagcc ctcttggtaa
     8041 actcccagaa acacaaagat aatcttctgc ttgtaatgct cttatttgat ttaaaaaatt
     8101 atggacagct gtctgcggta ttttaggtcc ttgattcact aatttgtatt cttctcccgt
     8161 ttgattgact tgtgtaaata cattgatacg cgtcatacca ggcacttcaa taaactctgt
     8221 ttggatttgt ttctgctgta aatagtcttc tatatacttt cctgtaaacc cagcagaaaa
     8281 acctaatgcc gtacttggga aatctagcat ttttaatact agtgacacat tcaccccttt
     8341 tccgtttgcc tgaatgtcat cctcttttgt gcggtttacc ataaaaggat gcatctcctc
     8401 tgtttcaata aatagatcaa ttgccaaatt catcgtgcat gtgtagatag acataaccaa
     8461 cgacctcctt gactctatta tgaaatacgc gaaagagaaa atgttttctt aatcgtccca
     8521 acacaaagaa aacctttaca tttataaact cgtcgtgcta ttttttaact aacttttggt
     8581 gaaagaagga aagaatatgg atcacaaatt aagtgaaaca gaacagtacc tctggaactt
     8641 tatcgaacac cacatcctag agatccctaa ctactccatc gtgaaactaa gtgaacaagc
     8701 aaatgtctca acagccacca ttgtgcggac aatgaaaaag aaaggctatg aaggatttac
     8761 ttcgtttaag caccatctaa aagaaggaga aaacaagaac atcaattttt ctttcttaga
     8821 taaagtagac aaaggtatcc gtcgagccat tctaaaaaat gaacaagaag tggtacgtac
     8881 gatcaatatg ttggaaatcg gaaatattga ggatgcaata caaagaatca aatttgctga
     8941 ccgggttttt gtctttgcac gaggattttc agaaatgatt ggtcaagaga tgctcgtaaa
     9001 acttcaatta acagggaaaa attgtcaaat gcatactgat ccagaaatca tcaaaagcat
     9061 cagtcggaaa ctaactaaga aagacagcgt tatttttgtt tctttgaacg gcgagacaaa
     9121 agaactcgtc accgccgcaa agaattgcta tgatgcagag attggttcga ttctagttac
     9181 agccaatcat tcctctacac tgaaagacta ttgcgagatc aatctcgtag gtttcaaatc
     9241 agaaggttct tatttccctg attatgaagt acggtcacgt ttgcctctcc agattatagc
     9301 gagaatcctg cttgacgcct atgtattgcg tatgggggag aattgaaaag cagtttatta
     9361 tggttggtta cagtttaaaa catcacattt tttattcttt atattgtatg
//
LOCUS       DAZUMI010000113         9406 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000113 DAZUMI010000000
VERSION     DAZUMI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.116"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG81C_002350"
     CDS             168..362
                     /locus_tag="ACG81C_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065489.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG81C_002351"
     CDS             631..930
                     /locus_tag="ACG81C_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065490.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG81C_002352"
     CDS             complement(1027..1461)
                     /locus_tag="ACG81C_002352"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5065491.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG81C_002353"
     CDS             complement(1693..2403)
                     /locus_tag="ACG81C_002353"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5065492.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG81C_002354"
     CDS             complement(2393..3253)
                     /locus_tag="ACG81C_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5065493.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG81C_002355"
     CDS             complement(3258..3896)
                     /locus_tag="ACG81C_002355"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5065494.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG81C_002356"
     CDS             complement(3911..4210)
                     /locus_tag="ACG81C_002356"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5065495.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG81C_002357"
     CDS             complement(4253..5737)
                     /locus_tag="ACG81C_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5065496.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG81C_002358"
     CDS             complement(5757..6218)
                     /locus_tag="ACG81C_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065497.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG81C_002359"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG81C_002359"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5065498.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG81C_002360"
     CDS             7521..8291
                     /locus_tag="ACG81C_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5065499.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG81C_002361"
     CDS             complement(8564..9391)
                     /locus_tag="ACG81C_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065500.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2919 a         1927 c         1481 g         3079 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttccc
//
LOCUS       DAZUMI010000114         9399 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000114 DAZUMI010000000
VERSION     DAZUMI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.117"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG81C_002362"
     tRNA            2..74
                     /locus_tag="ACG81C_002362"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG81C_002363"
     tRNA            78..150
                     /locus_tag="ACG81C_002363"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG81C_002364"
     tRNA            155..225
                     /locus_tag="ACG81C_002364"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG81C_002365"
     tRNA            238..311
                     /locus_tag="ACG81C_002365"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG81C_002366"
     tRNA            323..394
                     /locus_tag="ACG81C_002366"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG81C_002367"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG81C_002367"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5065501.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG81C_002368"
     CDS             1983..3455
                     /locus_tag="ACG81C_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5065502.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG81C_002369"
     CDS             3538..4326
                     /locus_tag="ACG81C_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5065503.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG81C_002370"
     CDS             complement(4393..5061)
                     /locus_tag="ACG81C_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5065504.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG81C_002371"
     CDS             5176..6888
                     /locus_tag="ACG81C_002371"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065505.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG81C_002372"
     CDS             6891..8663
                     /locus_tag="ACG81C_002372"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065506.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9399)
                     /locus_tag="ACG81C_002373"
     CDS             complement(8745..>9399)
                     /locus_tag="ACG81C_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5065507.1"
                     /translation="NGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRY
                     SLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELS
                     SNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSS
                     HMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2821 a         1575 c         2036 g         2967 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattt
//
LOCUS       DAZUMI010000115         9265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000115 DAZUMI010000000
VERSION     DAZUMI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.118"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG81C_002374"
     CDS             136..309
                     /locus_tag="ACG81C_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065508.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG81C_002375"
     CDS             949..1422
                     /locus_tag="ACG81C_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5065509.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACG81C_002376"
     CDS             1431..1658
                     /locus_tag="ACG81C_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065510.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACG81C_002377"
     CDS             2294..2665
                     /locus_tag="ACG81C_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065511.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACG81C_002378"
     CDS             complement(2921..3163)
                     /locus_tag="ACG81C_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5065512.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACG81C_002379"
     CDS             complement(3195..4097)
                     /locus_tag="ACG81C_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5065513.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACG81C_002380"
     CDS             complement(4110..4298)
                     /locus_tag="ACG81C_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5065514.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG81C_002381"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG81C_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5065515.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACG81C_002382"
     CDS             complement(4913..5806)
                     /locus_tag="ACG81C_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5065516.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACG81C_002383"
     CDS             complement(5884..6807)
                     /locus_tag="ACG81C_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5065517.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACG81C_002384"
     CDS             complement(6856..7206)
                     /locus_tag="ACG81C_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5065518.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACG81C_002385"
     CDS             complement(7239..8141)
                     /locus_tag="ACG81C_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5065519.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACG81C_002386"
     CDS             complement(8134..8991)
                     /locus_tag="ACG81C_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5065520.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2607 a         1749 c         1517 g         3392 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaatt
//
LOCUS       DAZUMI010000116         9117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000116 DAZUMI010000000
VERSION     DAZUMI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.119"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2773
                     /locus_tag="ACG81C_002387"
     CDS             5..2773
                     /locus_tag="ACG81C_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5065521.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            2854..3273
                     /locus_tag="ACG81C_002388"
     CDS             2854..3273
                     /locus_tag="ACG81C_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065522.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3285..3755
                     /locus_tag="ACG81C_002389"
     CDS             3285..3755
                     /locus_tag="ACG81C_002389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5065523.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3772..4551
                     /locus_tag="ACG81C_002390"
     CDS             3772..4551
                     /locus_tag="ACG81C_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5065524.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4538..5359
                     /locus_tag="ACG81C_002391"
     CDS             4538..5359
                     /locus_tag="ACG81C_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5065525.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5376..6425
                     /locus_tag="ACG81C_002392"
     CDS             5376..6425
                     /locus_tag="ACG81C_002392"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5065526.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6438..7442
                     /locus_tag="ACG81C_002393"
     CDS             6438..7442
                     /locus_tag="ACG81C_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5065527.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8075..9094
                     /locus_tag="ACG81C_002394"
     CDS             8075..9094
                     /locus_tag="ACG81C_002394"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5065528.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3037 a         1532 c         1928 g         2620 t
ORIGIN      
        1 taagttgttg aagaaggaaa ttttatgtta cttagagaac cagacagcct ttttcgattt
       61 agaacatatg aacgaggtct ttacagcaca aaggttagct gatcattttg gggtaaaaag
      121 aaacacagtc agtcattatt tgaaccagtt aaatgatgag caactactag tcaaaatcaa
      181 ttctcgacca gtgatttatt tccacaaaga agcttttgaa aaacaatttt ttaagttacg
      241 cacgaatttt tatttttcta tcgatgatct caaagaggaa cagccttttt tcgcgcaacc
      301 aaaagactta ttctctttta tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca
      361 aatcaaaaca gctctttact atccagatgg cggattacca cttttgatta caggcgaaag
      421 tggcacaggt aaaagttatt tagttaattt ggtctatcag tattgtctgc ttcatgatct
      481 gctagaagat tctgcaccat tcatcacttt taattgtgca caatatgcag ataatcctga
      541 acttttgact agcaatttgt ttggacatgt taagggagcg tacactggtg cagaagaaaa
      601 taaaaaaggg gcttttgaag cagcagatgg tgggatcttg tttttagacg aggtacatcg
      661 cttaacacca gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg tgatttatcg
      721 tatgggagaa cctaatttgt cacgccggat caaaacacgt ctcttttttg ctacgactga
      781 agagattact agtcatttct taacaacttt cattcgaaga atacctatcc gaatcgaact
      841 acctgcactt aatcagcgta gtcgtaatga acggctagaa ctgatttact ctttttttct
      901 cgaagaacaa agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt tgtcactttt
      961 aacaagtcag aaattatccg gaaacatagg agagctgaaa aatatcgtca aagtttcagc
     1021 agcaaaagca tatgctgaac agcgtgaaca gcctgaaatc catgtcacga tccatcattt
     1081 gcaaaaagaa ctacttgcgc agcctattac taaaaataca gcacagcaag gaatctatat
     1141 tacgcaagac cagaccttag agcaattaat ggagaagcaa cagcccgaac agcagcgaat
     1201 cgttaaaagt tttgagcaaa tcctaattga tttcaagaaa aataactgtc tgctatattc
     1261 ttgcgaagga aaattaaagc aagaagtgat tcaattattt gattttttgc tgtttgagac
     1321 cagtagacaa gaaaaacacg agctgctggt ttattggaca caatctatcc gagagacctt
     1381 tcgacaaatg gaaactgcct atcaaatcaa attcgatgga aacagtgtct atgccttgag
     1441 ttactatctc tatcagcgaa gcactatcaa atggttaccg gaagaacatg atattatcca
     1501 actaatcaaa gaattagaac aacaagtttc tcagtcttat ccatctagtt atcactatgt
     1561 ccaacgaatt ctggaattaa gtaagatcaa attagatatt gaagacacaa gtatggatcg
     1621 gattttactg acgatctatc taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa
     1681 agcgatgatt gctgctcacg gttatgcaac tgctagcagt atggcaaatg tagtaaatcg
     1741 tttattacaa gaggatctat ttgaatcatt tgatatgcca ctagatgtca caccacagca
     1801 gattgcttca gaaatccttg attattgcga aaacagcgat gtttcaaatg gattgatcat
     1861 attagtagac atgggatctc tgaaagaaat ccatcagttt tttaaaaaac agttatccgt
     1921 accgcttttg attttgaata atgtgacgac acctttagca atcacagtag gggaatgcct
     1981 gcaaaaaaat gtatcattag aagagatagc agaagagact gttcgacaga tccagccaga
     2041 atggcgactg ctctatccag aggaaaacaa acccaaagca ctcattacta cttgcttcac
     2101 tggaatcggt acggcggtcc acttaagtga actgctagaa aaaagcttac ctacggcttg
     2161 tcagttgaaa atcatcccat atgaatatca gcaattaaaa gataagaaaa acagtgaccc
     2221 attattttcc atatacgaag tcgtaggaat gattggcacc acagatcctg atatcaccaa
     2281 tatcgactat ctttcattag aagacctcat ctcgggggaa aagatgtctg tactagctga
     2341 atggttggac actactatga atgcttcaga gaaggaaata ttcaatcagc gcatcattcg
     2401 taatttttca ttggaaaaag tactggattc cgtgactatt ctagatacag agaaggtgat
     2461 gggagaaatc gatcatttta tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa
     2521 tcccaaaaaa ttagcgttgt atgtccatgt gagctgtctc attgaacgat tgatccgaca
     2581 aatgccaatc gaaacctatc aaggattaga gaaatggaag cagtgtcaaa aagaagggtt
     2641 atctgctatt aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca tgatccccaa
     2701 ttctgagatt gcctatatct atgatatttt gagtgaagat acagagtttc tctcgataga
     2761 agaagaattt tgaacataag caagttgttg ccactaattt tggcatgcaa cttgcttttt
     2821 tagtatgtgt aagtgaaaag aggagatgaa aaagtggata gagaaattat cttagcttcc
     2881 catggaaaat ttgcttcagg gatattagat tcattagagc tgatctatgg gaaaaatcat
     2941 tctattactg tattcgattg ctatactgat gagaattttg atttagcgga aacagtaagg
     3001 cagcttttta tgaggtacaa agataaagaa atcattgttc tgacggatct ttttggcgga
     3061 agtgtcaaca acgaattctt acaatatatc aatcgggatc atgtctatct cgttgccgga
     3121 ttgaatttgc cactattgat tgagctagta tctcggttgg acgctgaagt agagacaact
     3181 gttttgatcc agcaggtgct aactgattca aaaaaaatga tccagttttg taacgagcgg
     3241 ctcaatcaga aaatagaaga agacgaattt taggaggaag aaaaatgatt gtattgacaa
     3301 gagtcgacca tcggttatta cacggacaag tagctttttc atggacacaa acaataggcg
     3361 cagactgtat tttgattgct aatgatgatg tacctacgaa tgagatacga aagacaacga
     3421 tcaaattagc caagccacaa ggagtcaaac tagtgatcaa atcaattgat gactcgattg
     3481 ccgcgttgaa aagcggggtg acagataaat ataagttatt catcgtagtg gaatcgatcg
     3541 aggatgcata taagctggca gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga
     3601 tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt aaatatttta ccagaagagg
     3661 aggaacgctt gaaacgatta attgacaaac aaatagaagt agaaatccgt caagtaccag
     3721 cagataaaaa agtggatgta agagaagttt tgtaagaaag gaagaacctc aatgattgta
     3781 caagcagtct tattaggaat tgttgcattt gtcgcacaat cagaatatgc actagggaca
     3841 tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta
     3901 aaaactggga tcattatggg cgcaacttta gagttagcat ttatcggatc gttctcagta
     3961 ggtgcttcga ttccacctga tgttgtgacc ggtgggattt tagggacggc ttttgctatt
     4021 acagctggag ccggtacaga aaccgcgctt ttactaggtt tgccgattgc tacgttgaca
     4081 ttgatattga aaaatattta cttagggctt ttgattccaa ttatgaacca taaagcagac
     4141 aattatgcag aagaaggaaa ttataaagga gtcgagcata tgcacttgct tgccggtatc
     4201 ggtctttcct tgatgcttgg tgttgtggta atggtttctt atatggtggg aagcaatacg
     4261 atcggaaatc tattaaatat gattcctgac ttcgttcaaa aggggttgtc agtagctaca
     4321 gggttgattc cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt
     4381 gcaccatatt ttttcttagg ttttgctata gcttcttact tagagattcc tgtgaccgga
     4441 gtagctattt ttggtgcgat tttagcagtt gtggtagtca atattatgaa tgtccgtcaa
     4501 ctaccacttc aaacaactgc aggggaggta gaagaagatg aagaattctg agatcaaaaa
     4561 cagcaaaata acgaaaaaag agctaaatca cgtattttgg cgttcttttc aaatggagtt
     4621 ttcttggaat tatgaacgtc agatgaatat ggcttatgtt tatgcaatga ttccgatttt
     4681 gaaaaagtta tatcagtcga aagaagaaat ggcagcagct ttgaaacggc atctagagtt
     4741 ttttaacacg accccacata tcgttacact gattttagga atcacggctg caatggagga
     4801 agaaaataag gatgatcctg aatttgatgt gactgcgatc gatagtatca aaacatcttt
     4861 gatgggacct cttgcagggt taggagattc tttcttttgg ggaactttgc gattgattgc
     4921 cacaggtgtg ggtacctctt tagccatgca aggaaatatt ttaggcccta tcttgttcat
     4981 tttgattttc aatattccgc acattctgtt tcgctatcta gcaacaggtt ggggatatcg
     5041 cttaggaacg ggttttttga aaaagatcca ggcaaacggt atgatggaaa gtttgacatt
     5101 aggcgcgtct attattggac tgatggtcgt tggagcaatg actgccacga tgatcaccat
     5161 caatattcct ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa gtatttttga
     5221 tgatatcgtt ccaaatatct tatgtcttgg agcatttggc atagtgtttt atctattgaa
     5281 gaaagaagtg aaaccattaa cgatcttgat cggactagct ttctttggaa tttttggttc
     5341 gtggataggg attttttaaa aaaggagcag agaaaatgtt tacgatgcaa gattatatct
     5401 atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa tgatttttcg acaagagaac
     5461 atatgaaaaa aacagtgaat ttactgatct tagcaacggg ttcatcttat aacgcttgtt
     5521 tagcagcgaa gccagcattg gaatcttatg gtgatcttac tgtcgatatt caagaacctt
     5581 tttatttttt caattatgga aaactttctc cttcaatcga tactgtgatt gctgtgtcac
     5641 aaagcgggaa aagtgcttct actgttgaag cagtaaaaat gatacaaaaa caagggttac
     5701 ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc tttagaagct gcacaaatta
     5761 ttgatttagg tgccggggta gaatcagtag gcttcgttac aaaaggctat tctgcgactg
     5821 ttttacagtt actactctta gggataggaa ttggaataag taaaaataaa atcagcaaaa
     5881 aaatagaaca agactacatg caacagttgc gcaaaataat caaccattta ccagcgatta
     5941 ttcaaaaaac ggaagaattt tttgatggat atcaaagttt atttcgttta gcacagcgtt
     6001 tcgtcaccat tggatatggt ccaaactggg gaacagctaa agaagcagag acgaaattga
     6061 ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga agcttatatg cacggcccat
     6121 acctggaggc agatgcttcg catttgctat tctttattga aggggaaagt gtaaataagg
     6181 aacgttcgca aaagttgcaa agatatatga gtcggtacgt tggagagact ctcaccatta
     6241 ccacaaaaaa agcaagaaac gagaaaacac ttggattagc aatagaatgt gatgaatatc
     6301 tgtctgtatt agcgcttgtc gttccgtttc aactattcgc ttataaaacc gcagtagcta
     6361 aaggtattga cttgaacaaa aagatttttg aagactttga tacagtcttg aaaagtaaat
     6421 tataagaggt ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa gcaataaaag
     6481 gcgcactagc tttacgtccg caagtagaag aaatcatcga taaactttat gcggaaaaat
     6541 ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca
     6601 cttatatgaa tgggaaaagc aacttgcctg tctttgtaca gcatgctgca gaatattaca
     6661 ctactggaaa caaaagatta acaaaagatt ctatcgttat tttgtcctct gttaccggta
     6721 caactcaaga agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg acactgattg
     6781 gatttatcga taaagcgaac agtaagttgt ctcagctatg cgattttgtg gtcacttatc
     6841 cagctcctgg aacagaacaa atcaaatttt ttatggcagc agatcgattg atgtatttga
     6901 atggagaatt cgaagcttat tcagaatact atgaacagct cgaacgttat ttaccaacag
     6961 ggttagtgga agcagagaaa aaagcagatg catttggctt atcatttgca gaaaaacatc
     7021 gacgtgatag tatgcattat tttattggag cagggaatca atggggagct gtttattcat
     7081 atgccatgtg ttactgggaa gaacaaagct ggcttccatc gaaatctatc catgctgccg
     7141 agtttcttca cgggacgtta gagatcgtag aagaaacaac cccagtcaca ttatttttag
     7201 gcgaagatga acagagagtg ttatctgaac gtgtggctaa acttttgcca aaaatttgca
     7261 gcaattatac attgatagac acaaaagact atccagtaga aggtatcagt gaaaaatatc
     7321 gcggacgtgt tttgtctttc ttattgatgc atgtagtgac acaacgcatc gatgctcatg
     7381 tggagcagtt gaactgccat ccattagaga tacgccgcta ctatcgtcag tttgattatt
     7441 aggatagact ggataaaaag aatataaaaa ataaagagag cagcgataac tgctcccttt
     7501 atttttttaa aatattggca tcaattacgt tataaaacac attttttgca ttatctacag
     7561 ttcaacttat aatctgttta atgggacatg tgggtgccaa ccattttttg ttatgcaata
     7621 gtactattta gttgttgcat ggatccgtag taaaagtcca agtacaacga gttgcgccag
     7681 tttacatagc gttttgtatc cagaatgttg actaaaacaa aagttcctat gtcaaccgtc
     7741 aacagagaca atctgtccgt tgcaggacct gacagattta gaaatgttca atctgtctct
     7801 tatgtatttt aaatcaatac tcactaggag aagtttccta acctcctaat agtaatgctg
     7861 gtagtaacat tgtcaaaact gcttcctctt aggaacaagc aaagcataac gagtgtaaaa
     7921 ataaaatgta gactattgta acatatcatt gattatttca agtaaaaacg ataaaagaag
     7981 taaacaaaca cttaaaaaca atggaaatta gtcccaactt taagaaatgt atactataat
     8041 ggaaattatc aattatttat ttggaggaat atccatgaaa caaaaaatcg cagttcttgg
     8101 tcctggatct tggggcactg ctttagcaca aacattggca gaaaacgggc atgatgtccg
     8161 tatttgggga aatgtacctg aacagattga tgaaatcaat aagtatcata caaatcagca
     8221 ttttttacct gatttgacga tcccagaatc gattatcgga tataaagaat tggcagaagc
     8281 agtggataac gcagatgcta ttttatttgt tgttccaaca aaagcgattc gttcagtcgc
     8341 acaggaactt attggtaaaa tgaatactaa gccaatcatt atacatgcaa gtaaaggact
     8401 agaacaagat acgcataaac gaatctcaga agttttggct gaagaaattc cagaagaaaa
     8461 aagacaggca attgttgtat tatctggtcc tagccatgct gaggaagtgg cagtacatga
     8521 tatcacaaca attacagctg caagtattga tcaaaaagca gcagcctatg tacaaaaact
     8581 gttcatgaat gattatttca ggatttatac aaataatgat gtgattggcg tagaaactgg
     8641 tgcagcactg aaaaatatca ttgcaattgg tgccggcgct atccatggat taggattcgg
     8701 agatgatgca aaagcagcaa tcatgactcg aggattggca gaaatcagcc gtcttggtgt
     8761 cgcgatgggt gccaatcctc tgacatttat cggtttgagt ggtgtggggg acttgatcgt
     8821 tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt aacttgttag gacagggaca
     8881 taagctggaa gaagttctag aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa
     8941 agcagcagta gagctagctc aacagctagg tgttgagatg ccaattactc aaacaatcta
     9001 taatgttttg tataatggag aagatatcaa aaaagcagca aaagaaatca tgttacgtga
     9061 cggaaaaatg gagaatgaat tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DAZUMI010000117         8958 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000117 DAZUMI010000000
VERSION     DAZUMI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.120"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..1384
                     /locus_tag="ACG81C_002395"
                     /pseudo
     CDS             170..1384
                     /locus_tag="ACG81C_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1563..1898)
                     /locus_tag="ACG81C_002396"
     CDS             complement(1563..1898)
                     /locus_tag="ACG81C_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5065529.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1882..2145)
                     /locus_tag="ACG81C_002397"
     CDS             complement(1882..2145)
                     /locus_tag="ACG81C_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5065530.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2605..3408)
                     /locus_tag="ACG81C_002398"
     CDS             complement(2605..3408)
                     /locus_tag="ACG81C_002398"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5065531.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3386..4510)
                     /locus_tag="ACG81C_002399"
     CDS             complement(3386..4510)
                     /locus_tag="ACG81C_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065532.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4526..5341)
                     /locus_tag="ACG81C_002400"
     CDS             complement(4526..5341)
                     /locus_tag="ACG81C_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065533.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5331..6203)
                     /locus_tag="ACG81C_002401"
     CDS             complement(5331..6203)
                     /locus_tag="ACG81C_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065534.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6288..7577)
                     /locus_tag="ACG81C_002402"
     CDS             complement(6288..7577)
                     /locus_tag="ACG81C_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5065535.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7721..8233)
                     /locus_tag="ACG81C_002403"
     CDS             complement(7721..8233)
                     /locus_tag="ACG81C_002403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065536.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8300..8443)
                     /locus_tag="ACG81C_002404"
     CDS             complement(8300..8443)
                     /locus_tag="ACG81C_002404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5065537.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2783 a         1900 c         1428 g         2847 t
ORIGIN      
        1 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
       61 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaagcaa aatcaattct
      121 accttagatg ttcttatatc actctatcag tcctatttga caaagagcca agaaaaagac
      181 gttggtcgtc gatgctgttc tctcgcctac tcctcgtgct tgtagaagtt gtggttctac
      241 tgtggtagat ggaaacggga aagcaattat agtgaaaaat gggaagaagg aaacgattgt
      301 ccgttttgaa caatacaatc atatgccttt ggttacgcgc ctaaaaaagc aacgctatac
      361 ctgtaaaaac tgtcgtaccc attggacggc tcaaagttat tttgtccgac tcagccattc
      421 aatcgcagat catgttcgat ataaaattgc ttctttactg actgaaaaag tatctttgtc
      481 ttttattgcg aaaagttgtc aggtatcttt gactaccgtt actcgtacat tgaaagagtt
      541 taaaagctat ttaccaaagc cttctaagaa gattctcccc agagtattga tggttgatga
      601 atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag atggtgaaac
      661 aggaaactta attgctgtct tgcctacgcg taaattacct cgattaacaa gctatttctt
      721 aggctgcacc aatccagaag aagtagaatt cttagtcaca gacatgaacg ccgcttactt
      781 ccagcttacc aaacgtgttc tgccaaatgc gaaagtagtg attgatcggt ttcatattgt
      841 caaacacatg aatcaggcac tcaatgagtt gcgtatccgt gaaatgaatg agcttcgtaa
      901 agcaggacag aaaagtcagg cagaaaaact gaaaaagaac tggcgctttc tgctaaaaaa
      961 tcgtgcaagc atcaaccatt atgagtacaa aacatggaaa agtttccgag caccaaaata
     1021 cccatttctc actgaagcaa tgatgattga tcgattgctt gagttttctg cgcctctaaa
     1081 ggaggcgtat cccttttttc atgaattagt cgaagccttt cgagacaaag accctgactt
     1141 atttttctcc ttattggcag aacttcccga aacgttagat gacggctttc gggagaagct
     1201 tcaaaacctt ctgacctatg aagaaggtat caccagcgca atgatctatc cttattccaa
     1261 tggaaaaatt gaagcgaaga atacccacat aaagacaatg aaacgagtat cctacggatt
     1321 taaatcattc gagaacatga gaattagaat ctttttgatc aatcaattaa tcaatgtaag
     1381 ataacaaaaa atctgaacca gaatgtggtt cattctgacc cggatttgat tttgcacaac
     1441 tcatcagtcc ttattgacaa agagccaaaa atctgagcca gaatgaggtt cattctgacc
     1501 cagatttgat tttgtacaac ccatcagtcc ttattgacaa agagcccttc tttttagtat
     1561 tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact taattcccca
     1621 atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt tcctacgtca
     1681 atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac gtatttcagc
     1741 tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga aattgtgtcc
     1801 agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc tgtgaaagca
     1861 acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt caattttttc
     1921 gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat tatggattcg
     1981 tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa caatttgctt
     2041 ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa tgctttctgc
     2101 ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct ccacctcttt
     2161 ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac tacttttgaa
     2221 ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc taaatggcct
     2281 tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac actcaacgac
     2341 acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt ctgaaaactc
     2401 cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag accgatatgg
     2461 ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt attgatagcg
     2521 attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa agtgaatgaa
     2581 gtctttattt ttaatcaata atttttaatg attaccagta attggttttt tcatgcgccc
     2641 tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc cgtcaattaa
     2701 actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt attcatctac
     2761 accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag cactgctacc
     2821 ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca attcagcaaa
     2881 accttccgga acttcagtta caatagatgg atcttcccac gacaatggat gatgaaccaa
     2941 taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa tcatctgtcc
     3001 tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa taattcgtaa
     3061 cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa cactcattcc
     3121 ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag gtgtggttgg
     3181 gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc cttcatgtac
     3241 taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt gtaacaattt
     3301 ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa acaagttagt
     3361 ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg ctattccctc
     3421 tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa tcaaaaaaat
     3481 gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa gtaggttttt
     3541 cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa ctttgaccta
     3601 atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa aaggttacgt
     3661 gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt ttttgccaag
     3721 ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca taagtaactg
     3781 gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt ttaggtttcg
     3841 tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat aaaacaatcc
     3901 ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa gtttgtttat
     3961 aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc acatccaaat
     4021 ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca cgagccaatg
     4081 ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg tatggcgtta
     4141 aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc ttaattttat
     4201 tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat aaagcataat
     4261 tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct agttgtgtac
     4321 ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc agcaaagtag
     4381 attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc acagctaaat
     4441 caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt aattcaagcg
     4501 tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa gtcccgataa
     4561 tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt aaactaatcg
     4621 ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc atcgccaacg
     4681 gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac tcattccaag
     4741 aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga ggaaaaacaa
     4801 ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct agaaggctca
     4861 ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca gcaaattgtg
     4921 gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac aaataattag
     4981 gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag taccagattt
     5041 tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct acgcctaact
     5101 taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg ataattggaa
     5161 gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc aaactagtaa
     5221 tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac aataatggat
     5281 agataataat gattatgatg aacataaata agagatgtgg caaccagcgt ttaattttca
     5341 tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc aaatattaat
     5401 aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa agccttttga
     5461 taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt gatgatctta
     5521 atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa atatagtcct
     5581 gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg tacacgatcc
     5641 aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa aatgacaatg
     5701 aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat tacccaacct
     5761 tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac agcaattggt
     5821 cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa agaaatcaca
     5881 gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac tattcctaat
     5941 aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac ggtataggac
     6001 aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac atagttggca
     6061 aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact caaatagatc
     6121 gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg cgccagatac
     6181 aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc aaaagcagac
     6241 gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta tttgagtaac
     6301 tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc tgataaaatt
     6361 ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc aggaaatgaa
     6421 ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg attttcagct
     6481 aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg taaataacca
     6541 gtaccttctg tccatttagc taaagattca acactataaa gatattttaa gaaatcccaa
     6601 gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc accagcaggt
     6661 agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt aaaagtacta
     6721 tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac gttaccatca
     6781 ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc ttttaccatc
     6841 ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc tttgccatct
     6901 tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt atcagcaggt
     6961 tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga aaaagtttga
     7021 acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa tatatcttga
     7081 tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt accatctttt
     7141 tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc attatccgga
     7201 tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc aaaataatct
     7261 ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac agaagcttgt
     7321 aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac cttcactttg
     7381 tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc tagggtatcc
     7441 gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt tgtcttggta
     7501 ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac ccctataaca
     7561 agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca tttttctaat
     7621 caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa ataattcgaa
     7681 atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag aataaagccc
     7741 ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga ttataaaata
     7801 gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag gttatattat
     7861 aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc catacagaaa
     7921 atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat ccttcatgaa
     7981 tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc aaatctgctt
     8041 cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa aatatatgct
     8101 ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt tgtggccaga
     8161 tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct tttaaatcac
     8221 agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc tcaagggtta
     8281 ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca tctgtttgct
     8341 gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga aatgcccagc
     8401 tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt ttgacccctc
     8461 ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt aatttcttgt
     8521 taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct tgcatgctcc
     8581 ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc aattaagtag
     8641 ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta ttccttcttc
     8701 ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc aatggcttat
     8761 tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa gtctttgata
     8821 caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag cgattggtcg
     8881 atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc ccacaaaaaa
     8941 agctgtcctc acagacag
//
LOCUS       DAZUMI010000118         8721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000118 DAZUMI010000000
VERSION     DAZUMI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.121"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..1194)
                     /locus_tag="ACG81C_002405"
     CDS             complement(238..1194)
                     /locus_tag="ACG81C_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5065538.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1298..1900
                     /locus_tag="ACG81C_002406"
     CDS             1298..1900
                     /locus_tag="ACG81C_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5065539.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1988..3487)
                     /locus_tag="ACG81C_002407"
     CDS             complement(1988..3487)
                     /locus_tag="ACG81C_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5065540.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3503..4027)
                     /locus_tag="ACG81C_002408"
     CDS             complement(3503..4027)
                     /locus_tag="ACG81C_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5065541.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4325..5074
                     /locus_tag="ACG81C_002409"
     CDS             4325..5074
                     /locus_tag="ACG81C_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5065542.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5065..5850
                     /locus_tag="ACG81C_002410"
     CDS             5065..5850
                     /locus_tag="ACG81C_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5065543.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5819..6535
                     /locus_tag="ACG81C_002411"
     CDS             5819..6535
                     /locus_tag="ACG81C_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5065544.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6637..7656)
                     /locus_tag="ACG81C_002412"
     CDS             complement(6637..7656)
                     /locus_tag="ACG81C_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5065545.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7675..>8721)
                     /locus_tag="ACG81C_002413"
     CDS             complement(7675..>8721)
                     /locus_tag="ACG81C_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5065546.1"
                     /translation="DIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILS
                     KVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQ
                     TTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEA
                     KNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQP
                     MDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTA
                     TNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVW
                     IVVLQMIHGIGLFS"
BASE COUNT         2609 a         1774 c         1578 g         2760 t
ORIGIN      
        1 cctatttttc gtttggctgg acttattacc gcagaggaaa gcttttgaac accgtttaag
       61 aaagaggttg tgagcctacc gttcagctcc gagtcacaag gagcaatttc aaaaaacagc
      121 ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag ttgacaggag aacaccgttt
      181 aaagaagagg ttgtgaaatc agctttttga cctgaatatt agctgaattc acaacctcta
      241 tttttggatt ttttgttggg agaattctgc cttgatttca ataaaacttt taaaagactg
      301 caaaaactgg aacaattctg ctcgatcctc gaattcaggg cgactagtcg gcagaggcat
      361 ttgccgatac aatacataaa cttcttcaat acgtgttaaa atttgtctgc catcatttga
      421 ctctgaaaat gtttctgcag tataaatcaa taaaccatag atatgttttc cataataagc
      481 tggttgctgg attctctcta gattttccag catgtcttta atcacattgg cctgtgctct
      541 tctcatagag aagtaagttt cgtagtatag cggctgtctg agccagtagt tttcttgatg
      601 ttctctggca tcttcttggc tttcgcgaat gaatgtcagt aaatgttcgc atttgatctg
      661 cacatcttct ttattttctg ataaaagaaa ttctgccatt tgtctcaaaa tatttcgaaa
      721 ttctttttcg atctgtattt ggttttcctt cagctgcttc gtacgatctg gcatgtaaac
      781 atttgcaagc aaagcaaata ctaatccaat cgccattaat cccgcttcat tgccgatcag
      841 ctgccaagac atgttttcct ccactaaata atgagtgacc aaaaccgaat ttactactat
      901 cccttcggtt aatttgaagc gagaggcaag cgggatgaac aggaataaaa aaagactgaa
      961 gccaaaaata gtatacccaa cagcaaaaaa actgaacagt gctgttgccg tagctaatat
     1021 aaaggctgcg attcgattca tgcccgtacg caatgtagag cgcttggtat ttcctacact
     1081 taataatgca atgatccctg cagctggcca atacaacagc gataagctac ccgctgtgac
     1141 catcgaaaag aagacactga tcactgtttt gactgttcgc attcctatac gcatttactt
     1201 cccccttttt tattttaatt atacttgaat aatgaaaaac tagctatcag acaaaagttt
     1261 aggtatgata gtaaaaagac caaaaaggag aatctctatg caatttggcc gttttcgact
     1321 aggtatgcgt acgatcaaaa gtgccttagc tgttttttta tgtatcttgt tttttcattt
     1381 gactcaacga ggaatcccga tgattgctgc attatctgct gttttttctt tgcgtcagga
     1441 cttgactaca accgtttctt ttggaaagtc taggatcatc ggcaatacat taggtggtac
     1501 attagctatc atttattttt ttgtcaaaag ctattttcac aatgattttt tggtagaatt
     1561 gatcttgctt cctattctag ttattattgt gatcgtcgtg tcagatggta ttaacaataa
     1621 ttccggtatc atttccgcaa tagctacgtt gttgctgatt gcgttgagca tccctcaagg
     1681 cgaatccgct ttgtatgcta tccaacgtgt actagatact tttataggga cattcattgc
     1741 aatcggtatc aatttctttc ttcgtcctcc tgaaacagaa aagaaggaag aactatcaga
     1801 agatttggtc gaactccaaa agaaagaacg attgctaaga acaaatttac aagagataga
     1861 agctaagatc caaaaacaaa agaaaaatag cgaggactaa ttttcttttg caataaaata
     1921 aaaaaatgga gagaagaact ttaaaaagtc ttcctccatt tttgattgaa cggtgaagtg
     1981 tactacttta ttcttccttc atcgagtttt gttctgcctc ttttggcgct tcatacatat
     2041 ttacttggct agtagtccca cgtgtcgaca aattctctac gatttccttc aagtcaagtc
     2101 ctgttgtcgc ttttagtgtt tcttgagtag aagaaagcaa atttgtggca taatttgtca
     2161 ctcggttcgc tccactggtt tctcctgaac tgcttcctgt atcgacaaca gagatttttt
     2221 cgatatttcc tagcggctgt gctgcttcgc gcatcaattg cggtagcata tcgataacca
     2281 tactcaagac agctgcttcg ccatattcct tgaaggcatt ggcgatcttt tgttttgctt
     2341 cggcttctgc ttttcctttt gctagtgctg cttcggcttc tgcttgtcca gctaaacgtg
     2401 tcttattggc ttccgcttct gccagtgctt ccacacggaa tcgttctgct tcggcttcag
     2461 tcacttcttt gactttttga gcttgtgctt cttgttcttt agcgtaccga tcggcatctg
     2521 cttttttctt cacttccgaa tcatattgct tttcacgacg tgtgatttct ttttcttcca
     2581 gttcgatttg tttttgacgt tctacgattt taatctgcat ttcctgttcg attacttgtt
     2641 gttgtgctcg ggcactttct aaattgtagg cttggtctgc ttttgcttta gcaacatctt
     2701 gttcttgttt gtaggttgct aatttcagtt ctttttcttt taatgattct gcgatctctg
     2761 tttgtcgctg taattcagct gcttgtgatt ctttttctgc ttgagctttt ttgatacgcg
     2821 tttcttttag agcttccgct tctgctatat ctgcatctct tttgacttgg gcgattcttg
     2881 gtttccctaa agagtcaaga taaccgtttt tgtctctgac ctctttgata gtgaaagaaa
     2941 cgatgatcaa ccccattttt gcaaggtcta cagaagcgac ttcttgtacg ctttgactga
     3001 acttatcgcg attttgatag atttcttcta cagtcataga accaagaatc gagcgcaagt
     3061 gtccttctaa tacttctctt gcttcatttt ccagttcttc tcttgtcttg cctaaaaact
     3121 gttctgctgc tgtagcaatc tcttctacag atgaaccgat tttgatgatc gaagtaccat
     3181 cacacatcac cggtacgcct tgttccgtat acacttcagg ggtggagacg tccaatttac
     3241 tcgataatag actgatccga ttcgaacgtt gaaaaactgg caagacgaat gcgccacctc
     3301 cacgcacaat tttgattttg ttattgcttt catcaatgtg cacatttttg cttcctaaat
     3361 aacttccact gatgatcaac gcttcatccg gttttgctgt ctggtacttc gctacaaaga
     3421 cgatcagcaa catcagtaaa atcaaagctg cgattgcgat cgcgataaaa acaactggta
     3481 tttccattta acacacgctc ctttaagctt ttatcatgct ttcataaggc atgacataac
     3541 agattctttc cttgatctca atgatcaata ctttcgttcc ccgctcagct gtctgttctt
     3601 gggggctgta aaaacaagct ggacgactaa tggaaccggt aacacttttc aactgaattt
     3661 ctcccatacc tcgtacaggg atcggtgtga tcacaacagc tacttgtcct tccagagttt
     3721 tttcagaaga cgacatcgtt gattcggcat gtttcatcgg catcaatata tagaaattaa
     3781 gcaaaaatac caacactatc gatatgcctc cactagcaat aaataaaaat aaagatgatt
     3841 gatctgttaa acgctcacct agaaaaccaa ataagcttgt gaaagttaac caagggataa
     3901 taagcatcgg atcaagcggg ccatcaaaat cgaatacatc tccaaaaata atcaatagga
     3961 ctgccaatcc tgcacaaatc agcaatgtat accaataaat cgtctcaagt gcgattcccc
     4021 ctatcatata cacgcctcct tccatttcta cgtctatcat accaatgttt gaaaattctg
     4081 tatagcttct tttaccgact cagtggtgtt ttttatagga aggttgtgtt tatcgttgac
     4141 aatttgttaa atttttgttt tcatgttaaa attttcacaa taaattcttt cgaggtagcc
     4201 tatgtttcct ttttttaaga aaaaaccatt aaagctccag aacacgatca tccttgtcgt
     4261 ttgtttctcc acattcgttt ctttgattgt ttctgccata ttgatcagga attttattct
     4321 ttcgatgaac cgatctagtg tgtcagataa aatctctagc attgctaaaa tcgttgccaa
     4381 tgataaactc atccaaaaaa agctgcagac tggtgacccg acacatgcga ttcaaggaat
     4441 aacaaatgaa atcatgattg catcgaattt agattttatc gttgtcatgg accctgaagg
     4501 cattcgatat tctcacgcag acccaagtta catcggtctt cctttctcaa gtcatgaaga
     4561 tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa aaagaaggaa tgttagggtt
     4621 cggcttccgc tatttcgtcc ctatttatta tgatcaagag attattggtg cagtttgtgt
     4681 tggcttaaca aacaacacga ttgacaatca agtcaaagat ggacaaaaaa tgattgtttt
     4741 ttcattgatt attggactta gcgtaggcat cataggcgcc gtttttcttg cccaaaaaat
     4801 gaaaaaagta ctgcttggac ttgaaccaca agaaatagca gaacagttaa gtgaaaaaac
     4861 gattatcgaa aatgaagtag atgaaggaat cttagccatc tcttctgata aaactatcct
     4921 cttgatcaat acacctgcaa aaaacatttt ctaccaaaca gagcagctat tggatttcga
     4981 agtaggaaaa aaatcagtga taatctatat gacgttttgt ttaaagagac gattgagcat
     5041 caaacgaccg ttcggactca atccatgtat ttaaatggga tagaaactat ttctagcgtt
     5101 acacctcttt ttagcaatga ccgtttttta ggagcagtcg caactttacg aaatcgatca
     5161 gaagtaatga aattaatgga ggagcttggg gggacgaaac aatatataaa ctccatgaga
     5221 gctcaaaccc atgaattcat gaataaactt catgtcattt ctggtttgat tgatttgaag
     5281 aagtatgaag aagtcaccca ttttatccag cagctaaatc aaaattataa aaaagagatt
     5341 ggccaagtct caagtctgat caaagtacct gcaattgccg gttttttatt aggcaaaata
     5401 aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa gcgattcttt tatccctaaa
     5461 atggagatgg atgactccat ccattcactt ttgcaagtgt tagggaactt acttgataat
     5521 gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac aggtccggat tcatttaaac
     5581 tatgacacag aagctgaagt attcatcgtg accgtctgcg atgacggtgt cgggatatca
     5641 gaagaaatct cagaaaaaat atttacgcta ggtttttcga cgaagggaat caatcgtggg
     5701 tttggattga atctcatcca ttcaatcgtc aaacgccacg gtggattttt agaattttcc
     5761 aaacaaacaa atggcggtac aatcgtctat ctagaattac caaaaaagga agatcaaaat
     5821 gatgacaaat gtcctgataa tcgaagatga tccaatggtt gcagaattga atcgtacttt
     5881 tgtggaaagc atccctaata tgaacgtaat cgaaagtttc agaaatgtag aagaaggaaa
     5941 aaaatatttc agacaaaaaa acgcagatct gctgctgctt gatgtataca tgcctggcga
     6001 aagtggtatc cattttttat cttggcttag acagcaagga aatgatatcc cagtgatttt
     6061 gatcactgct gcagatgata tcgaaaccat aaagaatgtg atccgctacg gtgccttaga
     6121 ttatttgatc aaaccgttca ctttgcaacg ttttcagctg gcgatcgaaa agtatcttgc
     6181 tttctctcag acaatccaaa cgactgaaaa agccgatcag aaattgatcg atcaagtatt
     6241 caaccacacc actcccattc acgaaatacc atcattcatc gatttgccta aaggtctgtc
     6301 aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa gaagatacta ttttttcaac
     6361 aaaagaactg gctcaaaaaa gctcactgtc acgtatatcc gtcaagaaat atttgaattt
     6421 tttagtagaa caagatatat tgaacgaaag aatcaaatat ttggaaatcg gccggccgct
     6481 aacctgttat gaattgacaa agaatgggcg gaaaaagctc acagattact gttgagtcat
     6541 cgaattatcc catacaaaat agtccaagta tacagaagta acaactgttt acttggacta
     6601 tttttaaaca ttttattgac gaatatccat gttttcttat cttaattgac gggctttagg
     6661 aagagatacc tcatttttcg tttttccagt ttctattatt tcacgaaagt tctcgaaact
     6721 tgtcgaaatc atatttgata atgcctcatc tgtatttgaa cctacatgcg gggttacaag
     6781 aactctaggg tacatttcta ctaattgctg gatagcaggg tctggaatcg tctcccatgg
     6841 cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa acatcggttg caaaaccagc
     6901 taaatgattg cttttcaaag ctcttaaaat agcttcatta tcttgaagct ctccacgggc
     6961 tgtattaatc aagatgctac cttttttcat ttgagctaaa aatccttcat tgatcatttg
     7021 atcgttttca cctgggatat gaggtacatg aatactgaca atatcgcttt tggctaataa
     7081 ctcttcaagc gagtggaacg tcaatacttt tttagcagct tctgtctgat gaatatcgta
     7141 gccgatcact ttggctccta gtcccttaaa caaaacagct tccgtcaacc ctatcctgcc
     7201 tgttcctatg atccctactg tgcaatttct gatttcacga ctaaacattt gctcatccac
     7261 tcgaaaatct ttgaaagatg ttcggatcgt cgtatacgca gtatttctca aaagcatcat
     7321 ggcaaaagtc aaagataact ctgctatcgc atttggcgaa tacgatggta cacgggctac
     7381 tgtcatacca aaatcagctg ccgcttgtag atcgatatga ttgactccca cagtccgtgt
     7441 aaaaacataa cgaatcccat attcctgcat ttttgcaaga ttggtctcat ctgctataca
     7501 atttcccctc aacaatacag catcatggtc ctttgctgac acgatattgt catgtgtcaa
     7561 cagttcttca taaagactga gatcataatg gtaaatattc aatttttcaa aaaaatctat
     7621 ctcgttcgga cgaacaccgt aacatgcgat tctcatactt tcttcctcca tttcttatga
     7681 aaataaacct atgccatgaa tcatttgtaa tacaacgatc catacaggtg tcagtaccac
     7741 agcaaaaata gttgacagta aggatgcatt ggaggctaaa agtgcctccc tatcaaaact
     7801 gatagcatag gctgctgcca ctgttgcagc tggtgtagcc atcatgataa cgattgttgc
     7861 cagtgcttca aaattgactg gtagaatatt cgttgccgtc aaaactgcta gaatcacgat
     7921 attcaatgca ggtacaaata ccactttgac tagactgtaa taccatgaag ttttttcaga
     7981 tgcagctttt ttgaagctta cttcacctaa tgttgcgccg attgctagcc acgccaatgg
     8041 tgaacttaaa ccagctaaat aatccatcgg ctgaaatagc cacggtgcag tctggtcaat
     8101 tcgcaaaaac gctacttggc ttatctctcc ttctgcacct gtaactgaca cttgtggcag
     8161 atacccttgg aacagccaaa tgaaaaggcc ggcaaaagta gcgataatga ttggattgac
     8221 gaacatcgct ttgatatttt tagcttccat cttcaagcca ctcattttta tgtatccata
     8281 agaatacaaa aagattcggt agccaatatt gaaaattgag gaatacatca cgccgacagg
     8341 accataaatc gcactgacga taggcgttcc gaaaaaggtc gtagaaccaa agatcgtcag
     8401 gacacgcaac gttgtttgct tatccccttt atagcttaag aaaaaaggtt ttgaaactag
     8461 aataagaata atataaataa ctaatcccca aatcaagaca ttgattcctt gcatcagtaa
     8521 ttctgtattg atatcttgca tgaaagctcg aaatgccagt gctggcaaag ccactgttaa
     8581 gacgactttc gatagaattt ttccaacaga ttcagaaaaa atcccctttt ttctacaaac
     8641 aaaccctaat aatataataa aaactgttga agtgattgca ctgacaatcg acatatctgt
     8701 caaagtcgat cgaaaaatgt c
//
LOCUS       DAZUMI010000119         8493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000119 DAZUMI010000000
VERSION     DAZUMI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.124"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG81C_002414"
     CDS             complement(<1..149)
                     /locus_tag="ACG81C_002414"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5065547.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG81C_002415"
     CDS             complement(593..1714)
                     /locus_tag="ACG81C_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS5065548.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG81C_002416"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG81C_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS5065549.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG81C_002417"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG81C_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS5065550.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG81C_002418"
     CDS             complement(4304..5215)
                     /locus_tag="ACG81C_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5065551.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG81C_002419"
     CDS             complement(5232..6239)
                     /locus_tag="ACG81C_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5065552.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG81C_002420"
     CDS             complement(6236..8365)
                     /locus_tag="ACG81C_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5065553.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG81C_002421"
     CDS             complement(8370..8480)
                     /locus_tag="ACG81C_002421"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065554.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1727 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAZUMI010000120         8381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000120 DAZUMI010000000
VERSION     DAZUMI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.125"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG81C_002422"
     CDS             476..1339
                     /locus_tag="ACG81C_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5065555.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG81C_002423"
     CDS             complement(1468..3090)
                     /locus_tag="ACG81C_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5065556.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG81C_002424"
     CDS             3339..3860
                     /locus_tag="ACG81C_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5065557.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG81C_002425"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG81C_002425"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5065558.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG81C_002426"
     CDS             5941..6759
                     /locus_tag="ACG81C_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5065559.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG81C_002427"
     CDS             complement(6862..7059)
                     /locus_tag="ACG81C_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065560.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG81C_002428"
     CDS             7228..7806
                     /locus_tag="ACG81C_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5065561.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG81C_002429"
     CDS             7901..8038
                     /locus_tag="ACG81C_002429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065562.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2533 a         1498 c         1643 g         2707 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca c
//
LOCUS       DAZUMI010000121         8312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000121 DAZUMI010000000
VERSION     DAZUMI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.126"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG81C_002430"
     CDS             complement(105..629)
                     /locus_tag="ACG81C_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5065563.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG81C_002431"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG81C_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5065564.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG81C_002432"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG81C_002432"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5065565.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG81C_002433"
     CDS             complement(2351..3031)
                     /locus_tag="ACG81C_002433"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5065566.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG81C_002434"
     CDS             3157..4026
                     /locus_tag="ACG81C_002434"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5065567.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG81C_002435"
     CDS             4052..4567
                     /locus_tag="ACG81C_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5065568.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG81C_002436"
     CDS             complement(4614..5177)
                     /locus_tag="ACG81C_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5065569.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG81C_002437"
     CDS             complement(5232..5411)
                     /locus_tag="ACG81C_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065570.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG81C_002438"
     CDS             complement(5488..5997)
                     /locus_tag="ACG81C_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065571.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG81C_002439"
     CDS             complement(6093..6473)
                     /locus_tag="ACG81C_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5065572.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG81C_002440"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG81C_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5065573.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..>8312)
                     /locus_tag="ACG81C_002441"
     CDS             complement(7873..>8312)
                     /locus_tag="ACG81C_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5065574.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2724 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt ga
//
LOCUS       DAZUMI010000122         8250 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000122 DAZUMI010000000
VERSION     DAZUMI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.127"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..1653)
                     /gene="cls"
                     /locus_tag="ACG81C_002442"
     CDS             complement(205..1653)
                     /gene="cls"
                     /locus_tag="ACG81C_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5065575.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1805..2689)
                     /gene="ftsX"
                     /locus_tag="ACG81C_002443"
     CDS             complement(1805..2689)
                     /gene="ftsX"
                     /locus_tag="ACG81C_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5065576.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2682..3368)
                     /gene="ftsE"
                     /locus_tag="ACG81C_002444"
     CDS             complement(2682..3368)
                     /gene="ftsE"
                     /locus_tag="ACG81C_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5065577.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3473..4574)
                     /gene="prfB"
                     /locus_tag="ACG81C_002445"
     CDS             complement(join(3473..4501,4503..4574))
                     /gene="prfB"
                     /locus_tag="ACG81C_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5065578.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4700..7234)
                     /gene="secA"
                     /locus_tag="ACG81C_002446"
     CDS             complement(4700..7234)
                     /gene="secA"
                     /locus_tag="ACG81C_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5065579.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7456..8007)
                     /gene="raiA"
                     /locus_tag="ACG81C_002447"
     CDS             complement(7456..8007)
                     /gene="raiA"
                     /locus_tag="ACG81C_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5065580.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8135..>8250)
                     /locus_tag="ACG81C_002448"
     CDS             complement(8135..>8250)
                     /locus_tag="ACG81C_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFS5065581.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2794 t
ORIGIN      
        1 tgctatactg gctttgaaat gtgtctcttt taatagcata tattagctta tttcttgctt
       61 tccccaaaaa acaagagata ccccaaagtt aatttcgctc atgcgagacg ctttctccat
      121 ttctgacgta ctctcagaaa tggccccgtt aaaaaaggac acgagatctc catctgttga
      181 tggttctcat gtccttttta tgttttaaag gagcggcgaa atcaatcttg ctaatccttc
      241 tttcatcttg atgattattc ctcggttttc atacatttct tttgtgagat gcgtagaaat
      301 tttcgagtct tcaaaaaaag cttttcttac acgtgaagcc attcgttcat cataaaccag
      361 tgtattgacc tcaaagtcca agcgaaatga acgaacatcg atatttgctg agccaactga
      421 aacaatgcca ccatcgataa tcatcgtctt tgcatgaata aatccattct catatgtttc
      481 gatccttgct ccatactcga tcagctctgc agcgaaagaa tacgttgccc agtaaacgag
      541 aagatgatct ggtttgttag gtatttggat atgaactttt acgcctgaaa gaagagccaa
      601 cttcagtgct tcatgaatcg atccatccgg tatgtaataa ggcgtttgga tcaatatctc
      661 ccgtttggct aacgaaatca ttttcaaata agtcattttg atttgttcat gttcggtatc
      721 tggtccgctt gtcacgattt gaacagctat atcaccagta gtgtgaatgg acggaaaaaa
      781 tttgggagaa tagcctactt cttttgtatg ctgagaattc cagtccatca aaaaacggtt
      841 ttgcaaggta tacaccgctt ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa
      901 tttctttacc aatcctagat attcatttcc aacattgaac ccacctgtat atccgatttc
      961 cccatcaata actacgattt tacgatgatt gcgataattg atccgaggat taatatacgg
     1021 aacaaataga ggaaagaaaa atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa
     1081 gctggaagaa acttgtgtgg aaccccatgc atcaagaagg actctgactt ttacccctcg
     1141 tcttgccgcc tctgtcaatg catctcgaac ttcttcacct aatgcatcac tgcgataaat
     1201 ataatactgg aagtggatat ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa
     1261 tttttcacga ccatctgtaa ataaagtgat ttcattttta gtcgtataga gcgtactttc
     1321 aaataccgtg agcatataga tcaattgttt cacatctact tgtccagtag gcggatgagg
     1381 ataaagccct cgcatcaatg cctgtttgtc ttcttcaatt tccaggttct taccgatttt
     1441 cccttggatc ttcaaatcaa agattttctc tttggaaatc cctcttccta aaaaaatata
     1501 taaaataaat cctacaactg gaatgaacat caaaacgagc agccatgccc atgtttgagc
     1561 agtttccttt ctttctcgaa aaacgatgat aaaggaaagc aaaatattca taatcaaaag
     1621 gatcgtaaaa aaattgtcaa cgataatttt cacggcttca cctcttcttc taccaactta
     1681 agaataccca agttgttttt taaagtaaag ggaaaaaagc cgcttccttc tattctccta
     1741 gccaataaaa aaagaaagct ggaacgaaac tgtccaactt tctcatttta tttatattgt
     1801 ggtattagaa tttcaagaaa cgtctcatag aaatgatcga gccaagtgaa ccaatgatca
     1861 caccaatgcc gaccattaaa agattgattt tccaaacgat attttgtgga tgaatcaatg
     1921 aataatggga acgtaataat gatggattga tcaattggaa aaaccatgca taaccaaatg
     1981 tgatgatcaa aattggaaga attgcaccga tcagaccgat ccagccacct tcaaggaaaa
     2041 acggccagcg aatatatcca ttttttgcac cgactaaacg catgatttga atttcacgct
     2101 gtctagacaa aatcgtgatc cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca
     2161 gcaatgccgc tgcaactaat ccccatgtcc gaactgcacc agcgatttgg aaaatccgat
     2221 ctgaggaagc tccaccgtaa tcagaacgag aaacattctt caaatctgct gcttcttctg
     2281 ctacttgttt tgtataagaa ggatcagtag cacttactac atacacatca tataacgggt
     2341 tattgtcacc ttcaaacaat ttccaggaat cacccatttc gttttggatt tttttcaatt
     2401 cctgttcttt gcttgaaaat tcaacttttt taacatgatc taagtcattc aattcttttt
     2461 ctagcttttt catctcttcg tcttttgtac cgatatctac gaatacagat acatcaacgt
     2521 ttccttctat atcctgagct aatttagttg cattcatgat aactgccatg aatacaccga
     2581 ctaaagtcaa cgtgatcgtt accgcactga ctgaagcaac tgtcatccag ccgttacgtt
     2641 tcaaactctt gatactttct aataaatggc ggaaaaaggt tctaatcatc gtatccatat
     2701 tccccttccg cttgatctcg aataatgcgg ccgttttcga tagcaattac tcggtgccga
     2761 atcgtattta cgatcgtact gttatgtgta gccatcacga cagtagttcc ttgggcattg
     2821 atccgatcta ataatttcat gatttcccat gagttttctg gatccagatt tcctgtcggt
     2881 tcatccgcga taagtacttt aggcgtattc acgattgccc gagcaataga gacacgttgc
     2941 tgttccccac cggataattc gctaggaaag actcgaactt tgtgtttcaa accaacaagg
     3001 tccagcactt ccattactcg ttttttgata tctcgaggtt ttctgccgat aacttgcata
     3061 gcgtacgcaa cattttcata tactgttttt cttggtaata atttatagtc ttggaacacc
     3121 acgccgattt ctctgcgtag ataaggaact tcgcggtttt tgattgctat tagatcatga
     3181 cctgcgacgt tcaaagttcc ttttgaagct ttttcttctc gatacatcaa tttgatgaat
     3241 gtagatttac cggctccaga cggaccaacc acatagacaa actctccttg ttcgatgaca
     3301 atcgagagat tgcggatagc ggtcgtacca tttgagtact tcttcattac atccttcatt
     3361 tcaatcatgg cgttctctcc atatcttttt cagtctttag ctattatagc atggcaatgt
     3421 ttcaacatgc ttgcaaaatc ttacaatttt atttcaattt aacaatgggt tcttattgta
     3481 gatgcatttt taaatagccg tcaataaata aatcaaggtc accgtccata actgcttgga
     3541 catttcctgt ctctacgttt gtccgatggt cttttaccat agaataggga tggaaaacat
     3601 aagaacggat ctgtgaaccc cagccgattt ctttttgttc tcctcgaagt gcagctgctt
     3661 cttgttcttt cttttccact tctaattgat aaagcttagc ttttaacatc cccatcgctt
     3721 gctcacggtt tttcaattgt gagcgttgtg cctgactagc tactactgtt cctgtaggca
     3781 agtgagtgat ccgtacagca gattctgttt tattgatgtg ctgaccacct gcaccacttg
     3841 ctcggtacgt atcgattttc aaatcatctg gattgatttc tatatcgatc gtttcatcaa
     3901 gctctggcat cacatctacc gaacaaaaag aagtatgacg tcgtttcgct gagtcaaaag
     3961 gcgaaatccg tacaaggcga tgaacacctt tttctgattt tagataacca aaagcgttat
     4021 gtcctttaat cagcagggtc acactcttga tccctgcttc atcacctgct tgataatcta
     4081 gtgtttctac ttgatagcca tgctgctctg cccaacgtgt atacatacgt aaaagcatgc
     4141 tgccccagtc ttgggactcc gttccgccag cccctggatg caattcgacg atcgcattgt
     4201 ttcgatcata aggtccgtct aacagcatag ataattcata cgtcttcatt ttttcatcta
     4261 atgcattgat acgtgtttct gcttcttctt gcattgctgc atcaggttct tcttgcagca
     4321 tctccaccat caattcgatt tcttctagtt cttcggcaag agaatggaat tggtcatagg
     4381 tttctttatt agcgttattt tcgctgatca ctttttgtgc ggcttcgctg tcatcccaaa
     4441 aaccaggttc agccattttt gcttctgctt ccgcgatgtc ttcttccagc tgatctaagt
     4501 caaagagacc tcctgaaact tgtgaccttt tcctgcattt ctgtcaggag atttcgaatt
     4561 tcggatattt ccatgttttt tccttctttc actatacttt tttcattata tctagtctaa
     4621 cagaatcagc aaataacaga aagaaggtga gacgaattct taaaaaaaga atttgtctca
     4681 ccaatcctgc tgtcagaaat tagtttgcat tttttccatg acagttctta tattttttcc
     4741 cgcttccaca aggacaagga tcatttcttc cgaccttatt cacatggatt ggctgttttt
     4801 tcgcgctagt attactattt tctttggcat ctccgtcttc tgagtgagaa gctgttcctt
     4861 gagaaacctg ttcacgctgt acgttttgac ggatctcagc tttcataaag attcgagtga
     4921 cttcatactc gatggcgcct accatatctt caaacatctt atagccttct gtttgatatt
     4981 cgactaatgg attgttctgt ccataagcac gtaagccgat tgactgacgt aattgatcca
     5041 ttgcatcaat atggtctgtc catttagagt ccacaacacg caagatcacg actttttcaa
     5101 attccaacag ttgctctgga ccatttagtt gagccgcttt ggtattaaat acttcttgtg
     5161 ctcgttcgtt tagatactct ttgatctcct gagctgtttt tccttcgata tctgcaagag
     5221 taatgctatc ttcatggaca agagtactgc cggcaaaatc gatgatactt tccaaattcc
     5281 aattttcttt ctctaattgt gtatgcgcat ctactacacg actgattgtc cgtttgacca
     5341 tgttcattag tggcttggat aagctttttt cttccatgat tacttcttgt cgctgtttat
     5401 agattacttc acgttgttca cgcatcacat catcgtattg taagacgttt ttacgtgtgt
     5461 catagttatt tccttctaca cgtttttgtg cagattctac ttgtttcgtc agcatcttgc
     5521 tttggataac agcatcttcc tcaccgatct tcatccgatc taagaaagct ttgattcgtt
     5581 ctgaaccgaa tcgtttcatc aaatcatctt ccaatgacaa gtagaactgt gacataccag
     5641 ggtctccttg acgtcctgca cgtcctcgaa gctggttatc gatacgacga gattcatgtc
     5701 gttctgtccc gataactgct agaccaccaa gttctcttac cccaagtcct aacttgatat
     5761 ctgttccccg accagccatg ttggtagcaa tcgtcacagc acctttttga ccagcattca
     5821 tgatgatctc tgcttcttta aagtgatttt tcgcattcag cacttcatga ggaatacgtt
     5881 cttgattcag catatctgat aataactcag atgtttcaac cgcaacagtt ccgactaaca
     5941 caggttgacc tttgcggtag cgatctttga tatcttgcac gacagcgtgg aacttgcttt
     6001 gtaatgttgg gtaaagcaaa tctggacgat catcacggat aaccggacga tttgtcggaa
     6061 tctggtatac ttggatgtta taaatttctc tgaattcttc ttcttctgtt ttagctgtac
     6121 cagtcattcc cgcaagtttc ttgtacatac ggaaatagtt ctggaaagtg atcgtcgcca
     6181 tggttttcgt ttcgtcttcg atctctacgc cttctttagc ttcgattgct tggtgaagtc
     6241 catctgaata acggcgtcca tccatgatcc gaccagtgaa ctggtcaaca atcagtactt
     6301 ttccgtcttg gacaacataa tcgatatcta aaagcatgat atagttggct ctcaatgctt
     6361 gatccaagtg atgggtcaat gcagtatttt caatgtcgta taagttatcc aaaccgaatg
     6421 tttcttctgc tttttcgatc cctgcttcgg ttaaactgat tgtttttgat tgtacatcga
     6481 ttttataatc ttcttcttct ttcaaacgtt tcacgaagtt gtctgctctt gtatataaag
     6541 ctgtggattt ttctgcttgc cctgaaatga tcagcggcgt tctcgcttca tcaatcaaga
     6601 ttgagtcaac ttcatcgaca atggcataat tcagcggacg ttgtaccatt tgatggcggt
     6661 aaacgaccat attgtctctc aagtagtcaa atcctaattc attattcgta ctataagtga
     6721 tatcacaatt ataagcctcg cgtttttcat ccgaagattt tgagttgata ttcaacccca
     6781 ctgttaatcc taagaaatta taaagttcgc ccatttccgt cgagtcacgg gttgctaaat
     6841 attcattgac ggtaactacg tgtacccctt cgccagtcag agcatttaga tatactggca
     6901 tcgttgcggt caaggtttta ccttcaccag tacgcatttc agggatattt ccatcgtgaa
     6961 gcacgattcc acccatcaat tgtacgtggt atggatataa acctaataca cgttttgctg
     7021 cttctcggac gactgcaaaa gcttccggaa gtaattgatc taatgtttct cctttttggt
     7081 atctgccctt gaattcagca gtttttgcct gtaattgttc atcagttaat gctgccattt
     7141 ggtcagcgta tgcttcaact ttttttgcaa tgctatctaa acgttttagt tctttcttat
     7201 cgttttcgat aagtgaacga atcaaattgg ccatgcgttg tgtaatctcc ttttccttct
     7261 gtgtattatc cagactatgt agactttact tttcatttta tcattagtta tagataattg
     7321 aaatatctta agcgtattaa agtaagaact tcataattta ttcatatttc atctaaaaga
     7381 ggaggaaact caaaaaaact tgcggcagct gtcagtagct gctgcaagtt ttcgttgtta
     7441 tttaagtaag gctgatcagt ctgtttcaat cagtccgtat tttccatctt tacggcggta
     7501 aacgatactt gtaccatttg tttctgcatc ttcaaaaata aagaagttat gtcccagcat
     7561 gttcatttgt aagactgctt cttcactatc cattggtttc aatgaaaggc gtttcgtacg
     7621 tacgatatct aattcagaat cattttcgtt ttcgtcctct tcattgaata aagcagcagc
     7681 atctgcagta ttcatgcccg tttcacggga tttacgatta atttttgttt tgaatttgcg
     7741 gatttggcgt tccaatttgt caacaaccaa atcgatactt gcgtataaat ctggagaagt
     7801 ttcttcggca cgtaatacaa gataaggtaa tgggatcgtt acttccactt ttgctgtttt
     7861 ttctgtataa acttttaagt tcacatgcgc cgttgcatcg ggtgcgtcac tgaaatagcg
     7921 ttctagtttg cctacttttt tctcaacgta gtcgcgaatc gcttcagtta cttcgatatt
     7981 ttcgccgcgt acattatatc taaacataaa aattacccct ttcgtctacc acaatcaaga
     8041 aggagaagga ccactctttc cctcttctta attctattat atcgaactct aggttatttg
     8101 caaattaaat cgctatcaaa tttccacatc tgattcatct tgccaaagaa atggttcgga
     8161 tgattttcgg ctgtgcgttc tgcaagcaat ctgctgcatg aaaaagcgtc tgtcctgttg
     8221 tatagacatc gtcaataatc aatatttttt
//
LOCUS       DAZUMI010000123         8113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000123 DAZUMI010000000
VERSION     DAZUMI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.129"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1458
                     /locus_tag="ACG81C_002449"
     CDS             4..1458
                     /locus_tag="ACG81C_002449"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS5065582.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="ACG81C_002450"
     CDS             1534..2694
                     /locus_tag="ACG81C_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFS5065583.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="ACG81C_002451"
     CDS             2807..3814
                     /locus_tag="ACG81C_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5065584.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="ACG81C_002452"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="ACG81C_002452"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFS5065585.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="ACG81C_002453"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="ACG81C_002453"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFS5065586.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="ACG81C_002454"
     CDS             5793..7388
                     /locus_tag="ACG81C_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS5065587.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="ACG81C_002455"
     CDS             7404..7718
                     /locus_tag="ACG81C_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5065588.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
BASE COUNT         2415 a         1534 c         1943 g         2221 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaattata
     8101 aaatttaggg ttg
//
LOCUS       DAZUMI010000124         8086 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000124 DAZUMI010000000
VERSION     DAZUMI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.130"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(156..612)
                     /locus_tag="ACG81C_002456"
                     /pseudo
     CDS             complement(156..612)
                     /locus_tag="ACG81C_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            777..3139
                     /locus_tag="ACG81C_002457"
                     /pseudo
     CDS             777..3139
                     /locus_tag="ACG81C_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3507..4430
                     /gene="truB"
                     /locus_tag="ACG81C_002458"
     CDS             3507..4430
                     /gene="truB"
                     /locus_tag="ACG81C_002458"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5065589.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4434..5375
                     /gene="ribF"
                     /locus_tag="ACG81C_002459"
     CDS             4434..5375
                     /gene="ribF"
                     /locus_tag="ACG81C_002459"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5065590.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5727..6512
                     /locus_tag="ACG81C_002460"
     CDS             5727..6512
                     /locus_tag="ACG81C_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5065591.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6525..7376
                     /locus_tag="ACG81C_002461"
     CDS             6525..7376
                     /locus_tag="ACG81C_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5065592.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7500..7952)
                     /locus_tag="ACG81C_002462"
     CDS             complement(7500..7952)
                     /locus_tag="ACG81C_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065593.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2719 a         1380 c         1713 g         2274 t
ORIGIN      
        1 caaaaactcc cgcaaatcct gtgaaatcag gggttgcggg atttttttgt tttcattaag
       61 acgtattacc taagagttcc atgactttag tcttgcggcg tgtcagctaa ccaaaaaaga
      121 gaaaaagaaa agtaaaaaat tcgactttct tttttctagt agtatataaa tttttgcata
      181 tagataaaga tcaagatacc gataacggaa gctagattgt aatctatttt agtgacagtc
      241 aggtatagcc aagtaactag aagatcagtg gaacctgcac cataaaagtt tgagtttgtc
      301 ggtccgttga cagttaaaag aaaaatcacg ttgaagttat tgatatttcc gattacgccg
      361 cttcgatttg gtcacgatcc aaattttgga taatccctgt agaaattagc atagtagctg
      421 ggataccaat tcacatattg acaaagataa cagagacttt tacataaaac ggatcaatca
      481 ggaaaggaat tgctcgatcc agccccagtt ttccagcaat gtgttaactg gcccagcgtc
      541 attcagcaaa ttatgcatga tgaggagcga aatgaactgc ggaacagcca ttgtgataac
      601 gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag gaaaaatttc gtggtttttt
      661 atacctgttc atttggctag atataaaatc caaacaaatg aacagaagtg cttattaggt
      721 ttcaattggg agtatctgcg ctagaataag agtaataatg aaaaggagtg ttatgtatgg
      781 attattctag taaagaatac tttgataaaa tgacggcttg gtggcgtgct gctaattatc
      841 tttctgttgg gcaactttat cttaaagata accccttact gagacgtaca ctgaaacccg
      901 aagatgtcaa aaaacatcca atcggtcatt ggggaacgat tcctggccaa aattttattt
      961 atgtccattt gaaccgagtc atcaataaat atgatttgaa catgttttat attgaagggc
     1021 caggacacgg cggacaagtc atggtatcga atgcttattt agatggaagt tacacagaga
     1081 tctatccaga agtaacggaa gatgagacag gaatgcaaaa attattcaaa cgattttcat
     1141 ttcctggagg cattgcttct catgcagcac cagaaacacc aggttctatc catgaaggcg
     1201 gagagcttgg atactctctt tcacatgcag tcggagctgt attagacaat cctgaagtaa
     1261 tctctgctgt cgtgattggt gatggtgaag cagaaaccgg tcctttagct ggaagctggt
     1321 tctcaaatgt ctttatcaat ccagtgatag atggagctgt tttaccgatc cttcatttaa
     1381 atggtgcaaa aatagccaac ccaacgatct tggcacgtaa atcagatgga gaattagcaa
     1441 attactttaa cggacttggc tgggaacctt tctttattga aggaaatgat tcagaaaaat
     1501 taaatcctgt tatggctgaa aaaatggatc aagcaatcga gaaaattaaa tccatccaaa
     1561 aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc aaaatggcca gtattgatcg
     1621 tacgcacacc aaaaggatgg accggtccaa aagaatggga cggggaacca attgaaggaa
     1681 ccttccgagc acaccaagta ccgattcctg tagatcaaga acatatggat catgcagatg
     1741 ctttactccg ttggctgaaa tcctatgaac cagaaaaatt atttgatgca caaggacgta
     1801 ttttagaaga aatcagagaa atcgcgccta ctggtgacca aagaatggca aaaaatccta
     1861 tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc agattggaaa aaatatactt
     1921 tgcaatttga aaaaccaggc tccgttaaag cagaagatat gaccgaactt ggtaaattcg
     1981 ttcgcgagat cattgagaaa aatccagaaa atttccgtat atttggacca gatgaaacaa
     2041 aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg ccaatggatg gaaaaaatcg
     2101 aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt catcgacagt caattatcgg
     2161 aacaccaaga tgaagggttc ttagaagggt acgttctgac tggacggcat ggttttttgc
     2221 aagttacgaa tcattcttgc gagtggttga ctctatgttg actcagcact tcaaatggat
     2281 gcggaaatca catgaccttt cttggcgaaa tgattatcct tcgctaaatc tcattgcaag
     2341 ctcaacagtc ttccagcaag atcacaatgg atatagccac caagatccag gaatattgac
     2401 acatttggca gaaaagaagg cggagttcat ccgtgaatat ctgcctgcgg atgcaaatac
     2461 attgctcgca gtcatggata aagcattccg ctcgtcagaa aaaatcaatt tgatcatctc
     2521 ttccaaacat ccgcgtgctc aattttactc agcagaagaa gcagccgtct tagtgaatga
     2581 aggcttgaaa atcattgatt gggcttcgac agcaaaagag gaggaacctg aattggtcat
     2641 tgctgctgca ggaacggaat ctaacttaga agcattagca gctgtaactt tgctgctgga
     2701 agaattccca aaactaaaaa ttcggttcat caatgtcgta gatttattga aattacgtca
     2761 tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt gatcaatatt ttacaaaaga
     2821 caaaccgatc ttatttgctt tccacggtta tgaaacattg gtccgcacga ttttctttga
     2881 tcgtcataac catcatttaa tgattcatgg ctacaaagaa aatggagata tcacgacacc
     2941 atttgacatg cgtgtagtaa atgaacttga tcgttatcat ttggcaaaag acgcagcttt
     3001 aaaaatcaaa ggaagtcaag cagaagattt tgcgaaaaaa atggatcaga aattacaaga
     3061 acatcaaaac tatattcgtg aaaatggtat tgatctgcca gaagtattgg attggaaatg
     3121 gaaaaatctt gaccaataaa atgataaaat cgatcccgca aaatagaatt ttttacattg
     3181 attaagaaac tgaaaaaata aataaaaaaa acggaaaaaa gactggaaga atggccagtc
     3241 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
     3301 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaagcataac
     3361 atgatttcta tttacgtgag catgaatttt gttacgtttt tattgccgaa aaataaaaac
     3421 cgttcattat gatgtcaaat tcgtcagtaa ttgatgcaat gtgttataat aattcagtta
     3481 aatcaaaaat gaaatggaga aaagacatgg acgggttatt acctttatgg aaagaacgcg
     3541 gaatgacaag tcatgattgc gtattcaaat taagaaaaat cttacatacg aaaaaaatcg
     3601 gacatggggg tacgcttgac ccagacgtag acggtgtgtt gcccatttgt attggaaaag
     3661 ccacaaaagt tatcgaatat ttgacagatt ctgggaaaat ctatgtaggc gagataactc
     3721 taggtttcag tacaacgacg gaagatcgat ccggagaacg ggtagaaaca aaaccagtag
     3781 atatgtcttt atctgaaaaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca
     3841 cccagatacc accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg
     3901 cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc
     3961 ggacaagtga attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag
     4021 aatgcgggaa aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt
     4081 accctgcgca catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaggatg
     4141 ctctgacatt agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc
     4201 tgccaataga aaaaggagtg gaacggttca aacgagtaga tattactgat gaagtatggc
     4261 agaaagtaaa aaacggtatg agacttgatt atactgtatt tgcgctccca gaaatgccgt
     4321 cagaagaaat tgctttgttt tataaaggaa aagttgtcag tatttatcag ccaaacccga
     4381 aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata
     4441 tcatcaagat cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag
     4501 tgctgggatt tttcgacggc gtgcatatcg gacaccagaa agtcataaag acaggtatgg
     4561 aaattgcaca aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcta
     4621 ttgtctttaa aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag
     4681 aagaaaaaat ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt
     4741 ttgctcattt agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat
     4801 atgctgtttc cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt
     4861 ttcctgaata tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag
     4921 gtgaaaaaat cagctctacc cgtattcgtg gattactaga cagcggacaa gtaaaagaag
     4981 cagcaaaact attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac
     5041 gaggcagact actaggattc cctacagcaa atgtcaaagt aaagagcacg gttcatttgc
     5101 ctaaagaagg tgtttatgtg tcggaaataa aaattgggga aacatggtat ccagccatgg
     5161 gttcaattgg acataatgat acattcggtg aaggacggga gctgactgta gagttgtata
     5221 ttttggactt taatcaagat atctatggtg aacatgtcgc ggtacgctgg aatcaatttt
     5281 taagagatca agtgaaattt tctagtgtag aggaattgat tgaacaactc aaggaggatg
     5341 aacaagcaac agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt
     5401 tatgtataac tagcaattac aataaaatgg gtgataaata agcccaggaa aggggctgtg
     5461 acaaaagtct tgtcacagcc tttttttcca aataaatggt ttttcagaag tagcttcttc
     5521 ggaaataagc cgaaatttct tgaagctgac cacttctgtc acaacctctt tctttataga
     5581 aataaaagga cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac
     5641 tatatatctt ggtgtacact cactttaagg cggaaataaa agttatccgt aataacaggt
     5701 ttaggtatga aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa gtcgcagtag
     5761 taactggtgg ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa
     5821 tgaatgtagt tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa
     5881 tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa
     5941 tacagcgttt acttgatacg gctttagaaa attttgggac actggacgtt tgggttaata
     6001 atgctgggat ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag
     6061 tgttaaaagt caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta
     6121 cagagaataa taaaaaagga aatatcatca atatgtcttc tgttcatgaa cagatcccat
     6181 ggccaacgtt tgctcattac gctgcttcaa aaggcggggt aaaattattt accgaaacgg
     6241 tagcaatgga gtatgcaccg aagggaatca gaatcaatag tataggccct ggtgctatcc
     6301 gtacacctat taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta
     6361 tgatcccact aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg
     6421 cttcagacga atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt
     6481 tatatccttc tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg
     6541 cattgatccc ggcagttggc tgggggatcc agccactgat tttgaaaaag attggtggca
     6601 gaccaacaaa tgagatatta ggaactggta ttggagcggt gcttgtcggc ttactcgttc
     6661 aattattcgc ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg
     6721 gtgcattttg ggttattgga caaacaggac agtatacatc tttcaacttg attggtgttt
     6781 ctaaaacgat gccaatttcg actgcattac agttagtcgg aacctcgttg attggtgtct
     6841 tcgcttttgg agaatggtct ggaagtatgg ggaaaatcat tggagcagca gcaatcattc
     6901 ttcttgtatt tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta
     6961 tcacaaaagg aattagtcta ttagcatcaa caagtattgg ttattgggta tacagtgcat
     7021 tgcctaaact agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct
     7081 ttttaggggc agtgatttat gtgattttcc gtaaaccaac cgcattttct gaagacaaga
     7141 gttggaaagc aacgatcgtt ggagccatct ttagtgtgtc agcattggct tatatttttt
     7201 cagcaaatga aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct
     7261 ctacattagg cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa
     7321 cgattatcgg attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat
     7381 aaagatggta aaatttcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt
     7441 ataaaaaaat agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt
     7501 taagatacaa gggaaatatc tgtctggatc aataattttt cattgatcca ttgactaaag
     7561 tttacgacat aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga
     7621 tcgtattgta aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat
     7681 tgttctcgga attgattgat tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg
     7741 attggtgcat ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg
     7801 tataggaact caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct
     7861 tttagttttc cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg
     7921 gctaattcat ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta
     7981 ctgacttagt tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc
     8041 attgtaaaaa tcaagtcaga aaattctaac ttttatttct ctaaag
//
LOCUS       DAZUMI010000125         7973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000125 DAZUMI010000000
VERSION     DAZUMI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.131"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..1747
                     /locus_tag="ACG81C_002463"
     CDS             209..1747
                     /locus_tag="ACG81C_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5065594.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1744..2709
                     /locus_tag="ACG81C_002464"
     CDS             1744..2709
                     /locus_tag="ACG81C_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065595.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2696..4018
                     /locus_tag="ACG81C_002465"
     CDS             2696..4018
                     /locus_tag="ACG81C_002465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5065596.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4081..4185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4259..5002
                     /locus_tag="ACG81C_002466"
     CDS             4259..5002
                     /locus_tag="ACG81C_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065597.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5017..6009
                     /locus_tag="ACG81C_002467"
     CDS             5017..6009
                     /locus_tag="ACG81C_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5065598.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6021..6758
                     /locus_tag="ACG81C_002468"
     CDS             6021..6758
                     /locus_tag="ACG81C_002468"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065599.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6748..7470
                     /locus_tag="ACG81C_002469"
     CDS             6748..7470
                     /locus_tag="ACG81C_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5065600.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7554..>7973)
                     /locus_tag="ACG81C_002470"
     CDS             complement(7554..>7973)
                     /locus_tag="ACG81C_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5065601.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2547 a         1385 c         1741 g         2300 t
ORIGIN      
        1 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
       61 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
      121 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
      181 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
      241 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
      301 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
      361 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
      421 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
      481 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
      541 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
      601 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
      661 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
      721 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
      781 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
      841 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
      901 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
      961 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     1021 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     1081 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     1141 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     1201 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     1261 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     1321 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     1381 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     1441 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     1501 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     1561 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     1621 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     1681 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     1741 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     1801 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     1861 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     1921 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     1981 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     2041 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     2101 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     2161 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     2221 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     2281 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     2341 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     2401 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     2461 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     2521 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     2581 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     2641 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     2701 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     2761 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     2821 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     2881 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     2941 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     3001 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     3061 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     3121 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     3181 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     3241 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     3301 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     3361 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     3421 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     3481 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     3541 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     3601 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     3661 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     3721 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     3781 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     3841 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     3901 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     3961 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     4021 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     4081 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     4141 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     4201 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     4261 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     4321 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     4381 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     4441 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     4501 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
     4561 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
     4621 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
     4681 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
     4741 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
     4801 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
     4861 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
     4921 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
     4981 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
     5041 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
     5101 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
     5161 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
     5221 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
     5281 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
     5341 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
     5401 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
     5461 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
     5521 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
     5581 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
     5641 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
     5701 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
     5761 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
     5821 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
     5881 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
     5941 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
     6001 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
     6061 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
     6121 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
     6181 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
     6241 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
     6301 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
     6361 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
     6421 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
     6481 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
     6541 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
     6601 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
     6661 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
     6721 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
     6781 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
     6841 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
     6901 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
     6961 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
     7021 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
     7081 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
     7141 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
     7201 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
     7261 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
     7321 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
     7381 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
     7441 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
     7501 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
     7561 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
     7621 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
     7681 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
     7741 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
     7801 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
     7861 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
     7921 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZUMI010000126         7747 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000126 DAZUMI010000000
VERSION     DAZUMI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.133"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG81C_002471"
     CDS             complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG81C_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5065602.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG81C_002472"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG81C_002472"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5065603.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG81C_002473"
     CDS             complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG81C_002473"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5065604.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="ACG81C_002474"
     CDS             5737..7038
                     /locus_tag="ACG81C_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5065605.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="ACG81C_002475"
     CDS             complement(7107..7706)
                     /locus_tag="ACG81C_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5065606.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2296 a         1588 c         1359 g         2504 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatat
//
LOCUS       DAZUMI010000127         7626 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000127 DAZUMI010000000
VERSION     DAZUMI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.134"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2672)
                     /locus_tag="ACG81C_002476"
     CDS             complement(<1..2672)
                     /locus_tag="ACG81C_002476"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5065607.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2659..3021)
                     /locus_tag="ACG81C_002477"
     CDS             complement(2659..3021)
                     /locus_tag="ACG81C_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065608.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            3240..4460
                     /gene="sstT"
                     /locus_tag="ACG81C_002478"
     CDS             3240..4460
                     /gene="sstT"
                     /locus_tag="ACG81C_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5065609.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4607..5242
                     /locus_tag="ACG81C_002479"
     CDS             4607..5242
                     /locus_tag="ACG81C_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5065610.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5433..6173
                     /gene="truA"
                     /locus_tag="ACG81C_002480"
     CDS             5433..6173
                     /gene="truA"
                     /locus_tag="ACG81C_002480"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5065611.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6318..>7626)
                     /locus_tag="ACG81C_002481"
     CDS             complement(6318..>7626)
                     /locus_tag="ACG81C_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065612.1"
                     /translation="YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALT
                     FLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQ
                     KIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNN
                     NYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVK
                     SLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHA
                     NKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSN
                     KLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVI
                     TYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2493 a         1195 c         1441 g         2497 t
ORIGIN      
        1 attttttgtg tcgcccatgt tgcactgtac tggttacctg caacaagatc atattgtatc
       61 agacgataat cttctagaat ttctttttgt ttctcagtta aatttttttc ttggataatt
      121 tgactatcct ctgtaacaaa gacctgtttt ccattaaaac gattagacaa gctttctgta
      181 ggatcaattg ttaatgcagg tagatgatta gatacttctg tcaacaacgc atagtaagga
      241 gttactttga tattcgcatg ctcaagagcc atagcaccaa agttattagg agcaacaact
      301 tctttattta acgtagctgc ttgttcacga ctatacttgt tgttataaat gaaataatct
      361 gtctggtgct gttctaatcc atattttttc gaatcattcc cactatagat tgcgggtaaa
      421 tggtcaccat aaaaaacaaa ggtaatcggt ttatcgattt tgtcgatttc ttggatgaat
      481 ttttttactg ctgtatcggt atagtgaagg ccttgcatat aagtttgtaa ttcttttctt
      541 ctattaggaa tgacagcagt tccttcagca gtgtagttca attcactata gtaatcatca
      601 taaggcatat ggttttgcat agtggataat tggatgaatt gtgacgtttc tgtattgctt
      661 tgtaaagctt tcaaggtttc tttgtatgct gaatcatccg atatgcgtgg gctttgttct
      721 attttatccg tataagttaa tttatccgga ctgtctaaat aatagaacct gtcaaaacca
      781 aatttatcaa acacttgttt tctgcgataa agagcagctg taaaaggatg gatggctatt
      841 ttttcatcga atagatcggt aatatttgga cttattttct gacgctccac tagctgggta
      901 taaggtgtaa ctaacgaagc agacaggtta ctgatactca agcctgtcag tccttcccat
      961 tccatgttag ctgttccgcc accataaccg acggataaca tcaatcctcc tgttccttct
     1021 gttataatgc ttttgatgta aggaataggg tctgtaggta ctgtcagatt agggacacgt
     1081 gcaggatcac tgaagctttc acttaaattg agaacgatcg tctgattgtt taaccagtca
     1141 cttcttgttt ggttgatttc atctgccttt gtgttgtact ttttcattat tccattaatt
     1201 ttttctttcg aatagccagc aggttcttcc atgattttaa tgtcgatatt gtttaggaat
     1261 tgaatgactg gtccgttatc ttttacggcg ccttgctggt taaagaaagt cttgtttact
     1321 ttaaataaat taaacagcaa gaaagaaggc gaatttttgt gattaatgaa aaagacaccc
     1381 gaaaaacatc ctaataatac agcgatactg ataaatcgac gtttccaatg aatcttcaag
     1441 gaatactgtt tttgtagtct acgttgtaca ataatactag taatcaataa taggagaagg
     1501 atcaatccgc ccacgattaa taagactggg ctaaccatcg ataatagttc tgacaatccg
     1561 gtcagtattt ttaaatctga aggaaagaca ggctcttccc gcatttccat tttgattact
     1621 gtcgaaatag tgagcagtaa gttaatgatc aaggtagcag tcaaggcgta ccaaaaacga
     1681 ttggaaatca acaatagtaa gaagaaaaat cccataataa tgatgattgt caggatcatg
     1741 ggtgcttgtc gctgagtaaa tatttttaag gcaacctgga tagtttcttt ccaaccttct
     1801 tttggttcta aaacgaaaat ctgcacgaag gtaaagcagt aaaaaaatgc tgcaactaaa
     1861 aaataacttt tatgaacaaa aagccaatga attctttttt tccaacgctg agttaattgt
     1921 tcaatcgaat caaaagcgaa gtattcgatg aaacgatgaa tgaaacggat tttttgtata
     1981 taaaataaaa agataactag gagggacact gctaataaat agcaaataaa aaactgcccg
     2041 atatttatca gccatgcctt ccctgagttt ggaaattcac gtttcataaa tgtagatacg
     2101 cgataagaaa ctaaagggaa attacatata acttgagtga caatcaaaaa tacagcaaga
     2161 aaatttcctc ttccttttat acctaattta tcagatagca tttctaaaaa taaaaacgaa
     2221 gagaaagtat aatacattgc gaagagagaa tatggtttag aaaacctcag cgctgtatct
     2281 gtatttcctc tgatgatgag tgaaatttgt gtcatcaaaa taatcaaacc gaataaaaca
     2341 atcaagctga taaatacttg aggaagctta tagcggacct tttctttcca gttccattct
     2401 ttcaaaccat aacccattaa aaataaataa aaaatccata aaatactctg tccattctgt
     2461 aatgcaaacg ggtctttatt aaatagagta gagcagccaa ctaatccaat tgttaaaaat
     2521 aatagtagat atttaattgt tgttttcgat aatttattta aaaatccaac tgtataagga
     2581 atactacaaa aaagcaaagt acaagaaaca gcataagtaa agtaattctg actgataggt
     2641 gttaggataa gccaataatc acgaatattc aagctgccag cttgtacgta aaaaaaagat
     2701 attgcaaact agtgtggcta ttaggaatat aaaggcaaaa cgacttaaat aatgtaaaac
     2761 ttttaaacgg gcgatttttt gatattggta acccatgtaa agtgggatca aatttgcacc
     2821 aataacaacg gaagtttgta aaaatgtata gatgtagaat cgaccagggt ttgttgcata
     2881 tgtaggaata gtgactgctg aaatcaatat acgctgtaaa accatcatta tacaagcgac
     2941 aaagattgtc aggaagccat aactctcctt ctttattttt ttcaagaata aacacctcac
     3001 aaaaaaattg gacaaaaaca taagaaaacg tttcctgata agttgcccat taataacatc
     3061 ctaacttaga atatctcaaa aaacaacgtg tagacagaaa aagtacaaaa ttagcaagaa
     3121 atggttgttt gtattgttag aatttttaga aaattatacc tttagtttct ttgcataaaa
     3181 tgatacaatg attgttaaaa ttgtgaaagg gtggtcattt gttagagaag tttttggata
     3241 tgtcgttagt tcaacgtatt gttgttggta ttttagtcgg aatattgttt ggtatctttt
     3301 tccccacatg gacatttatt tcaatattgg gaacgttgtt tgtcagttgc ttgaaggcaa
     3361 tcgcacctat tctggttttt ttcttgacga tggcgtcaat tgccaagcat aaaattggga
     3421 ataagacatt cgtgaagcct attttgatct tatatttagt gggaacattg ctttctgcac
     3481 ttgtagcagt tattgtaagt tcaattttta ctgttcctat tactttacaa gaaacagtat
     3541 ctgaaaaagc accgcaaagt ttggaaagtg ttttatctac tatgttgacg aatgtcacac
     3601 aaaatcctgt tcaatctctt attgatgcga attaccttgg cgtcttattc tgggctgtat
     3661 tgttgggact agcttttcga gcacgttcag aatcgacaaa agagttggtg gatcagatat
     3721 ctatagcgtt gtcacaagtc gtacaagtga ttattgcatt tgctccgata gggattttag
     3781 gattggttta tcaatcaatt gctacgactg gaatagctgg attggcagag tatctgcagc
     3841 ttttacttgt tttgatcggt acgatgctgt ttgtcgcatt agttgtgtac ccattcctca
     3901 cgtttttgtt tattaaagaa aatccctatc cattgatttt cttttgtttg aaagaaagcg
     3961 cacttcccgc tttctttacc cgaagttctg ccgcaaatat cccgataaac atgatgttgg
     4021 ctgaaagatt gaaattgaca aaagagtctt attccatatc gatcccgtta ggtgctacga
     4081 tcaatatggg cggtgccgcg attaccattt ccttgatgac gttgactgct gtacattctt
     4141 tagggattga agcacctttt gttttgaaag tccttttatg tattttgtct gctttagctg
     4201 cttgtggagc ttcaggtgtt gcgggcggtt cactgttatt gattccatta gcatgtagtt
     4261 tgtttggtat ctctaatgat gtcgcgatgc aaatcgtcgg tattggattt attatcggtg
     4321 ttatccagga ttcaatggaa actgccttaa attcgtcaag tgatctgttg tttactgcaa
     4381 taggagagct aggcgcgcgt aaaagaagtg gggaaaaaat caaattgaaa gattgtttag
     4441 atggattaag tgaacgttga acgttagata tttagtattt ttaaaatgat tcgagttttt
     4501 aatgagttat atagatgttc gtgtaaatag aggtttctgt taaatcaaga taaagtcgct
     4561 tataataaca attaaaagca taggtaattg ttattggagg cgtatcatgg attttttacg
     4621 taaatttggt tttttctttt tattattagg cgtgctgagt gattttttga ctccgtacgt
     4681 attaggattg tattatcctg aattagatca aatgaagagc gtgatcagtg tatttggaga
     4741 ggtttccagt cctgtaagaa aagcgtttct tatttggtct gtggtttcag ggatcttttt
     4801 catcctgtca ttgcctgcat tgtatgctgc atttgtaagt acatctcgtg tgttagctac
     4861 cctagttaca gcagccatcg cgttctatgg tataggtgat tgtatcttta caggtttgtt
     4921 cagtatcaat acccatgaat cttcgtggaa tgtctcgaca tgggtccata atatcggatc
     4981 aggtttaggt tacagtggtt ttttattgtt tccagttctt cttttcttga tctatcgaaa
     5041 aaatgggcaa atggacgtaa gccacttata ccttggactg tctgtgatca gtttgctttt
     5101 tgcggcgatt tatggactgg caagaattcc ttctataaat tcttggccat tactgaataa
     5161 aataggtttt tgccaaagaa tcagtttttt ctttaattat gtgcccattg cctggttgag
     5221 tatcttgcat ctcaaacagt aaaacttgag ccgtcaaatt gaacagtcag cggatacttc
     5281 atccggtggc tgttttttgt ttttctgaaa tatatatttc cacagttttc ttgtatagct
     5341 tctgttttag agaagaaagg agctatattt tgtgctacaa tcttaaaaag gtagagagaa
     5401 gaccaaatgg aagacagagg agccactaaa atatgagaaa catcaagttg acaatcgaat
     5461 atgacggaaa aagatattca ggatggcaaa gacttggaga tgacgataaa accattcaag
     5521 gaaaaatcga aaaagtattg catcagatga caaatgaaga gatagaaatt attggatcag
     5581 gaagaacaga tgctggcacc catgcgcgag gacaagtagc aaattttaaa acaaacacag
     5641 aaattggact ggcagaaatg atcgacttta tgaatcgtta ccttccgcgg gatattgtca
     5701 tcaaaagaat cgaagaaatg cctgagcgct tccattcaag atataatgcg gtgggcaaaa
     5761 aatacagcta ttatgtttgg aacaacgtga ttccttcagc atttgagcga aaccacagct
     5821 tttatttccc tcaagagttg gatatggaca agctgaatgc agcttgcgaa aaattagtgg
     5881 gtacacatga tttcattggt ttttctgcat tgaaaaaatc aaaaaaatcc acagtgcgta
     5941 cgattgatga attatctatc gaacgaaagg gagaaatgct tcattttact tttgtcggag
     6001 acggattctt atataaaatg gtgcggatta tcatggggac attactagaa atcggcacag
     6061 gtgccatgcc gatagaaaca atcgatgaaa tatttgaaag caaagtgcgg caacatgcag
     6121 gagaaaccgt ccctgcacaa ggtctgtttt tggatgaagt ttattatcga taaaaatact
     6181 ttgataatgg atgaaacgca tgtttcacag tataaatatc gatgttaaat aatgtatgaa
     6241 gcaaattaaa gaaatataaa aaaagacttt ttagcctgtt tatgtgtaca gctaaaaagt
     6301 ctttcttaaa taaaaatcta gtgagaatgt tgtacttctc ttctcggttg tttttgttta
     6361 gaaaaagtaa atcgataaag taaaaataaa atcaaagtaa cgatagataa atatcttgaa
     6421 gcaattgcta gttttgtgta ctgataagta ataacgactt tatttatacc tggaggaata
     6481 gttaattcag ttgtgccaaa cttagataat tttgtttgta ctagtttgcc attaatagaa
     6541 gccacttgcc ctttataacg ataaataggt gtcgtcaaga cagtattttt attattcgaa
     6601 ttatctaatt caattgtaag tttgttactc ataaaatgag ttgttggatt tatttcctgg
     6661 ttacctagta aatattgatc attattaatc atatcaggat gatagattga ctctttgttt
     6721 gcataatcag tatgattgta attttcagct atagcaacag cgtcttcagg tgctataagt
     6781 gttaatgaat ctttgttagc atggatagaa ttgcttactc ctgagagatg caatattagt
     6841 gcaagaaacg taaagaccat tattttccat gaattatttt tagtagaaaa aatataatag
     6901 atcactatac tcatcgtata ggctataaat aaagaagaaa aaccatttaa ccgccagaca
     6961 aattgtagac tcttaactgg tgtatggtta aaaagttgcc aaggaaataa attggttgaa
     7021 caaaaaagaa caaatacacc aagaaagaaa ataaataaat catcagcagt taattttttt
     7081 ataaaaataa aagcaccaat caatccgaga aataagaata atccaatagt atagttgtta
     7141 tagttattat ttaaaatgtg tgtcagcata tcactaggag ccataccact taattctttt
     7201 cccagtggga ctttcaattc ttgtgctcta gattgttgaa taaaaggaat taagaatccc
     7261 gcacttaaaa aaaaggtgag gacggtagct tttaaaagac ttaagagtct agcgattttt
     7321 tggtcccaaa aataaaaaga aagaaacaac gttcctccaa taaatacaga aaccatagcg
     7381 acagaaagta aatgagtata cactactagc gtcatgccaa tagacaacca gtaccatttt
     7441 tggtaatctc taatataaat ttcgtaacac cccattagta tcaaaggcaa aaatgttaaa
     7501 gcgacagctt ctcccaatga agcacgatgg aatatgtcaa tggcacgata ggcggaaaat
     7561 gtataaataa tcgaaaataa cagcgaaata taacgattgt tttttatttg tttcatagaa
     7621 aaataa
//
LOCUS       DAZUMI010000128         7616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000128 DAZUMI010000000
VERSION     DAZUMI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.135"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..743)
                     /locus_tag="ACG81C_002482"
                     /pseudo
     CDS             complement(45..743)
                     /locus_tag="ACG81C_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            822..998
                     /locus_tag="ACG81C_002483"
                     /pseudo
     CDS             822..998
                     /locus_tag="ACG81C_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1174..1743)
                     /locus_tag="ACG81C_002484"
     CDS             complement(1174..1743)
                     /locus_tag="ACG81C_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5065613.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1817..2140)
                     /locus_tag="ACG81C_002485"
     CDS             complement(1817..2140)
                     /locus_tag="ACG81C_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065614.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2294..3106)
                     /locus_tag="ACG81C_002486"
     CDS             complement(2294..3106)
                     /locus_tag="ACG81C_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065615.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3433..4308
                     /locus_tag="ACG81C_002487"
     CDS             3433..4308
                     /locus_tag="ACG81C_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5065616.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4322..4645
                     /locus_tag="ACG81C_002488"
     CDS             4322..4645
                     /locus_tag="ACG81C_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065617.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4642..5394
                     /locus_tag="ACG81C_002489"
     CDS             4642..5394
                     /locus_tag="ACG81C_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5065618.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5384..7555
                     /locus_tag="ACG81C_002490"
                     /pseudo
     CDS             5384..7555
                     /locus_tag="ACG81C_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2265 a         1507 c         1375 g         2469 t
ORIGIN      
        1 aatagttcaa cttttggggt tcagttcaaa gaggggggga ttttttatcg ttgtggaata
       61 tttaaatacc aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga
      121 tcgtttgtcg cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa
      181 gatacatgat agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa
      241 atcccatcag ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata
      301 tactcgtatt tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt
      361 tctccgaact gatcaatgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca
      421 cctgctcgag ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgta
      481 aaaccatgag ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa
      541 ccgtaaggat ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt
      601 cctggttggt tataaggagt gtctaatgtt aatggaatac catatttcat tgctgaatct
      661 cttgccaatt caacgtatac tttatagttc ttttcaaaag tttctttatc atgtgtatgt
      721 tgtaactcga tttgcacagg acttttggct cacgaggttg tcctactcta catcttgctg
      781 atagatatgc tgaaaggaaa acaccaacat tatgttttgc tttttcagct agaacgagac
      841 aagcaggaat acctttagaa gatatcaaag gttttttagg tcataaagat gtttccacta
      901 cacaagtcta tgcccatatt tctcccgaag tgaaaaaaag atcaatgaat caactagaaa
      961 actatataga agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc
     1021 actaaaacta tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa
     1081 tctagcgaaa aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag
     1141 cctggcttgc ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt
     1201 gatgatgatt ttatccatca attgagattg actgttttgc tctcctatga tttcagctat
     1261 gattgtccga ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa
     1321 ctcgctaagc atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc
     1381 taccattcgt tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg
     1441 taatgtttgg gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt
     1501 aataggttct tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag
     1561 gacatctagt tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac
     1621 tttttcacga ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc
     1681 catcggcgat cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc
     1741 caatggattt cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat
     1801 cttttttcca aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc
     1861 tttcgatacc aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg
     1921 gaaaaaaatg atcgatgatc actagatctg cataccgagg cggcttattt cgagtacata
     1981 catgtataga gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc
     2041 ttgcttcttg gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat
     2101 ctaaataccc tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt
     2161 gtgcaagtgc ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa
     2221 atttttctaa tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa
     2281 ttacgtaacg aagtcattct tggtaaagta cttttgtcag ctttgatttg ctttcgctct
     2341 caaattcgct attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc
     2401 gatagtcttg tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca
     2461 agcgcaaaat tgctagataa tttttttctt ttattggcaa aatctgctcc tctctaaata
     2521 gcaattcagg aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg
     2581 ccggatcctt tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat
     2641 catctgcaaa aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc
     2701 tttctgaacc aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc
     2761 actttctcaa tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata
     2821 gcagattttt cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag
     2881 gttcgctata tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgaa
     2941 tgggacagtc gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt
     3001 tcagttcttc agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg
     3061 aaaataaatt ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac
     3121 ctgatttcta aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc
     3181 cctttctatt tatttaaata cgcaaatctt cctcattatt ttttatttta ctcaatttaa
     3241 tcgtattctc atttcaaaac gaatgtactt taaaaaaaca taataagatc actttgcttg
     3301 taggatgtat tcccttccat tacaatgaaa taaatagcga ttttatgaga aggtgatgtg
     3361 aaaatggaag aaaaaataga agaatccaaa caaaaaatcg atcagttgat ataagaatta
     3421 gaaaaagcgg tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca
     3481 ggtggtcatg tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca
     3541 ctagcaaaag ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct
     3601 agtgatattc ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg
     3661 ggaccgatct ttaccacgat cttgttagca gatgagatca atcgtacgac tcctcgtact
     3721 cagtcagcac ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac
     3781 cctctagaca atcatttttt tgtcttagct acgcaaaatc caatcgagta cgaaggaaca
     3841 tatcctttgc cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct
     3901 tcttttgatg acgaactttt gctacttttg gataaattcg aaaaagtgtt ggataactta
     3961 aatgaagtat tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt
     4021 gtcagtcccg aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagtcatgct
     4081 gccatccagc ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct
     4141 tatgctttaa tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct
     4201 tacgtgtttt cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaagaaa
     4261 caaatcattc atactcttgt tgcacaaatt cctattcctg tgaggtagat tgaggtagac
     4321 aatggaaaaa atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat
     4381 tgtgcttttt gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac
     4441 tctttatgta tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac
     4501 ttgtacattg cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc
     4561 aaaaaagaga actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt
     4621 tgcagaaaaa aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt
     4681 tggcagccaa aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat
     4741 agtctaggac ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg
     4801 cctgcttttt gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag
     4861 ctgaacgcgt accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat
     4921 tctttcaacc gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa
     4981 tacgattatc aagaagaaga atgttctcct cttttttgct tttggggttc gcccagtcca
     5041 aaatttgaac ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag
     5101 ccggaacttc tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat
     5161 tttgcttctt tggaagctgg cgatgagaaa gcttttttcg cacacttgaa aaaatatgca
     5221 aaaaaacaaa tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa
     5281 acattgtcaa aagatcgaac ggtttgtctt gtttatttcg tagaaaaaca actcatggtc
     5341 caagaaaaag acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaacggga
     5401 aaaatggctg cgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt
     5461 agtagctaat caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct
     5521 tattgtctgc tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat
     5581 aggttgtttg tttttatatt ttccttcgaa gacattttcc atattatgga tcttacagtt
     5641 tttttcgtca gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc
     5701 agaaaagaca gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt
     5761 ggattacgac tattggtacg caccctttct ttctttgatg gcttacttca tgatgcttac
     5821 cgtttttcgg aaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt
     5881 gtatctggca gcaaggcaat ttttctccat ccggttgttg tcgggaaaca ctcgattcca
     5941 gtcattgact acagttctgc ttatttgttt cttaactttt agcgctatct tgcctttata
     6001 tcttccgaaa gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa
     6061 tgatgaaggg ctttatgaca cactccatga gtatcaatta accggaagtg caagaacagg
     6121 cttcagtgag aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt
     6181 tacagcgaac caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg
     6241 ttctggatgg atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga
     6301 catcacgagc taccaaaccg ttatagaaga caaagaaaac attacattga ccctaaaaaa
     6361 acaagttttc ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag
     6421 tggacaaacg ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc
     6481 gacacgttct gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag
     6541 cagtcagtta aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag
     6601 tttgcctaaa cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat
     6661 tcgtaaagtc gaagctgtct aacagtattt aaaaacaagc ggaaatttcc gctattccaa
     6721 aacggatacc gggcatactc caaaagaaca cgattatgtg gattatttcc tctttgattc
     6781 aaaagtcggc tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg
     6841 tatcccagct cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca
     6901 agggaatgag gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt
     6961 tcctggattt ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc
     7021 acaaccagtt gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag
     7081 cgaaaatatg gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac
     7141 aacactcaca ccagcttata aaatcgtctt attcagcctt ggagtgtgtt tatccgtctt
     7201 gttgttcttt ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct
     7261 tcattttttt gcttctacta atacgattgc atatcaaaaa cttcttaacc ctttggaaac
     7321 attggtaccg cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga
     7381 attcaagcta gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg
     7441 acgacaagca gatccggtaa aattcgataa aaaaacctat ttagaaataa cgcatataat
     7501 aagaaaaatg gtatacaaaa aaattctgac aaaagggagc cgcttcaaaa aataagataa
     7561 aatttctgga gaatctactt ctggaatgcc gagaaccagc tgattgagta tcgatt
//
LOCUS       DAZUMI010000129         7599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000129 DAZUMI010000000
VERSION     DAZUMI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.136"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG81C_002491"
     CDS             complement(137..427)
                     /locus_tag="ACG81C_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065619.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG81C_002492"
     CDS             complement(545..805)
                     /locus_tag="ACG81C_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065620.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG81C_002493"
     CDS             complement(942..1355)
                     /locus_tag="ACG81C_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5065621.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG81C_002494"
     CDS             complement(1811..2296)
                     /locus_tag="ACG81C_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065622.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG81C_002495"
     CDS             complement(2435..2650)
                     /locus_tag="ACG81C_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5065623.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG81C_002496"
     CDS             2874..3674
                     /locus_tag="ACG81C_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5065624.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG81C_002497"
     CDS             3693..5561
                     /locus_tag="ACG81C_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5065625.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG81C_002498"
     CDS             5554..6045
                     /locus_tag="ACG81C_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5065626.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG81C_002499"
     CDS             6033..6587
                     /locus_tag="ACG81C_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5065627.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="ACG81C_002500"
     CDS             6605..>7599
                     /locus_tag="ACG81C_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5065628.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAZUMI010000130         7543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000130 DAZUMI010000000
VERSION     DAZUMI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.137"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..751)
                     /locus_tag="ACG81C_002501"
     CDS             complement(89..751)
                     /locus_tag="ACG81C_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5065629.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(761..1576)
                     /locus_tag="ACG81C_002502"
     CDS             complement(761..1576)
                     /locus_tag="ACG81C_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5065630.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1838..2281)
                     /locus_tag="ACG81C_002503"
     CDS             complement(1838..2281)
                     /locus_tag="ACG81C_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS5065631.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2415..2753)
                     /locus_tag="ACG81C_002504"
     CDS             complement(2415..2753)
                     /locus_tag="ACG81C_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5065632.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2741..3118)
                     /locus_tag="ACG81C_002505"
     CDS             complement(2741..3118)
                     /locus_tag="ACG81C_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5065633.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3342..4535
                     /locus_tag="ACG81C_002506"
     CDS             3342..4535
                     /locus_tag="ACG81C_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5065634.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4701..5123)
                     /locus_tag="ACG81C_002507"
     CDS             complement(4701..5123)
                     /locus_tag="ACG81C_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065635.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5494..6168
                     /locus_tag="ACG81C_002508"
     CDS             5494..6168
                     /locus_tag="ACG81C_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065636.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(6330..7502)
                     /locus_tag="ACG81C_002509"
     CDS             complement(6330..7502)
                     /locus_tag="ACG81C_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5065637.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         2342 a         1441 c         1297 g         2463 t
ORIGIN      
        1 tttagccata tgtttcaaaa aatgaaaaag aatgacagtt tttacataaa aactgtcatt
       61 cttaattgaa agtttcaatt tataaagtct acagatccat agttctcatc aatttacgat
      121 tgttgaagta aatcaaacca aatacgatag aaggacctag tagaatacac cattggggct
      181 ttacgaaaaa gaagagaaac aaacttgtca atagtaaaaa atctagtaaa ctcacatacc
      241 attttttcaa cgtatagtag aacgctatcc gtagaacttg atttggtttc gacgctggat
      301 ttctcacctg aaaataccag tagttgatgc taaaggaaac tgataaacaa gcaagtaaaa
      361 caaacaacgg aatcaaccat tttccataag aaaaacgtat aaaaaagaaa caatcaaaaa
      421 tcattatcac actaactatc caagcaaata gccaataaaa aaatcccttt agccaaaatt
      481 ggcgaaacgt ctttatgaac aaacgtactg gtgaaaaact tttttctttt gctaattctt
      541 ctgtcatacc tatcatcacg atgcaacctg gaccaataaa aagtaaagaa aaaataaagg
      601 aaaagacatt ccttccatct atcgacaaaa ggaacgcagc tgctatatac aagatattga
      661 cacaccaaaa acaaaggcta cttgccaatg attgatacaa ccaataaaag aattccatat
      721 aaacattttt gtcaaaacgc tgctctttca taatcacctc ctattccttc attcctgatg
      781 aagcgatccc ttctacaaaa tatttttgca tcgctaaaaa tataaaagca acaggaagaa
      841 cagaaacaac tgaggcagcc atgattaatg cgtactctgc atcaaatagc ccaataaaca
      901 ttttcaaccc taactggatt gttttatttt ccgtagaaga caaataaatg aatggcccca
      961 tataatcgtt ccatgtatta acgaatgtga tgatcgtcaa actggcaatc gccggttttg
     1021 taagaggtaa aatgatcttg cgatagattc cccactctga taagccatcg atccgcgcac
     1081 tctcacataa agaatctggg atactactgt agtattgctt caacaagaac acaccaaaag
     1141 cattgaacgc ttgtaatagc accaacgacc acaacgtata tgttaaaccc acttttctca
     1201 tcataatgaa ctgcgggatc atataagact gccaagggac agcaatcgtg ccgatatatg
     1261 ctaaaaataa aatatcgcgc cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac
     1321 tagaagtaaa caactgaatc aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa
     1381 atgtcactaa aggaatacga gaccaaataa ccagataatt ctctaaatgc caagttttag
     1441 ggatccattg aatcggaatc gtgaacactt cattatttgc tttgaatgaa gcagaaacca
     1501 tccataaaaa aggaatgatc gtcaataaag ctaatacaat cagcagaatc atacccaacc
     1561 attgaataaa gggcatgttt tttgaacgaa tttttgcttt ttttctttca tttcgaacac
     1621 ctccaaagtt ataatcttca tttctttgta atgattacaa tccagaacaa cggttattat
     1681 agctgattca tttccgttct tcttatgttt gtaagcgctt atttaacaag ttatgtaaat
     1741 ctctttattt tttttgattt aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc
     1801 gcttttgtat tgaaatttct tcgattagag atacttatta ctgttcaaaa gcttctttga
     1861 aacggtgaat caattcattg actacaggtg ataaaatccg attctttttc cagactagca
     1921 tgcagttcgt ttcaatcatt gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac
     1981 ttacagcacc ttctatgact aaagcagcac ctacctgatt ctcaattagc gcaaatacat
     2041 tgaaaatcaa attgtacgtt cctgtgatca gcaactgctc cttttttatg cctaaccagt
     2101 cagcaaacat tcgctgtatt tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt
     2161 cttcttttgt tatcacgtct ttttttgcta aaacagaatt ttctaaaatg agcaatcccc
     2221 atttttcctt tcttggtaac aaaatcgttt cgtatttctc tgtatctact ggatctagca
     2281 aaatcgctaa atctaacagg cttttttcta gtcactccgt gatatcatca cttgttccgc
     2341 tgtggatatg aaattgaacg ctgggataat cacggatcaa cttttctagt aatagtgcca
     2401 atgtattcga attatcagct tccacacagc cgatcgtaaa tcggccgctg aataattgtt
     2461 tttttctgtt ctcgaattct tgagtggttt gattcgttaa atctaaaatt tcttctgctc
     2521 ggctttttta gaaaataacc tgcttctgtc aagatcattt tgcggttttc gcgtacaaac
     2581 agttctgttc ctagttcctc ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta
     2641 agatgtagaa cttctgcggc ttgagagatt gttccttctt ctgcaattgt ccaaaaatat
     2701 tttagtaagc gtaactccat agcgatgctc cttgattttt ttacttgata cattccattt
     2761 tatttctaga taacacacga tcatatttct tgatttttcc ttgtaaacgt tccaaacatt
     2821 cttgcatttc agcaattttt ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc
     2881 gctcaaatac agttgcttct ccttcgctag ataggcgaat atattcaatc aaagactcga
     2941 tcgataatcc ggcatttcgc atacagatcg taaagtcgac ccattttagg tcttcttctg
     3001 tataatcacg gattccgcct tttgtccgat taactggtgg aatcaacccg attcgttcat
     3061 aatagcgcaa tgtatcggcc gaaatgccct tttcttcact gacttgtttg atattcatct
     3121 gatcactctt tctcttaatt tcttgccact ttacttatgt attttattta ttctacaact
     3181 ttaagtgtac tccaagtcaa tactcatttc tattaaaact aagctggatt atctatatcg
     3241 gttgtttgtg tattatttca aataaaaaag tcttgttttt ttattctttt ttggatatac
     3301 ttttaacaat ttagggaaat tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa
     3361 agttattatt atcgacatcg gcgggaattg ctttagagaa tatggatatt atgtttttag
     3421 ctttttcatt atcttcaatg attacttctt ttcatattag tggtacgcaa gcgggtttca
     3481 tttcaacgat cactaatttg gggatgctca ttggaggtat tttttttggc atcatagctg
     3541 ataaatatgg acgtgtgaaa gtattttctc aaacggtcct tctgttttcg attgcttctt
     3601 tattgatgta ttttgcttca aacatctact tggtttatct ctttcgcttt atcgcaggtg
     3661 taggagcggg aggagaatac ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa
     3721 aacaaattgg acgagcttct tcggttgctg gtattggtgc gcaggtcggt gcggctttgg
     3781 ctgcgatttt agctgctgtg atcattccaa cgtttggttg gaagatgttg tatgtcgtag
     3841 gtgtactgcc ggtgttgatg gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat
     3901 tcaaatctgt acaatctgcc gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac
     3961 atcttgcttg gcaaacatgt ggccttagta tcatggtaac tgtccaaatt gccggctatt
     4021 ttgggttaat gaattggctg ccttcaatta tgcaaaatcg attagggctt tcggtttctg
     4081 ggtcttcatt gtggatggtc agtactattt taggcatgtc tttggggatg atgacttttg
     4141 gcataatcat ggataaattg gggcctaaac tagccttttc tttgttttta atttgttcat
     4201 ccttatctgt ctatctactt gtgttagccc atagccagtg gagtttggta gcagccgctg
     4261 tgatagtcgg ctactttatc aatggcatgt atggtggata tggcgcagtg attagtagtt
     4321 tgtatccaac agaaatccgc gcgacggcga ataatttcat tatgaacctt ggacgagctg
     4381 ttggtggttt ttcttctatc gtcatcggtt tcttgatgga tcattatagt ttaactgccg
     4441 taatcttgtt tttgagcggt atttacctag tcagtctgct ggtcttgaca actcttactg
     4501 gtgttacagc attaagggaa agcttgcagc attgatagtt gagtctgtaa atgacaagaa
     4561 agaggtgtgg caaaagcttg catctgcggt aataagacca gccaaatcaa aaacatgaga
     4621 cactgtttcc gtttggctgg tcgtattact tgatgcaggg cagctttttc aactgctaat
     4681 ttccttctat ctggcggcta ctattctttg acgtccgtca tttcaaagta ctttactaat
     4741 aacttagcat aacgaaaaac tgtggataca ctcgtatatt gagcgtctgc ataatcttcc
     4801 aaatttttct caggatgttg atacatgaat tcaatcaaat gataattata tgattttctg
     4861 tagaaatgaa gataaataat gtagattggg aagttctcct gcttttccat agaaaagcgc
     4921 cgattattct cattcttcaa aatgacctta tcaggatatt tttggaacaa ttcttctaaa
     4981 tctgttagat aattcaagat agacttactt gataccttca attcgttttg tagttcttct
     5041 attgaacacc aacgttctgt ttcaagcagt aaatgaatca ttttaatttt cttttgagaa
     5101 acttttgtta aaaaagtctg catattttct cctcctgtat ctactttact atactacata
     5161 caagaaaaaa aacggataca aatatcatat tgtcacaccg tcaatagtgc ataaacctgt
     5221 tattatttct ctttttttcc taagtattct attgatatga atcatttaat cgtttattat
     5281 ataaatgtca ataatctaat aataaaataa cgtacatatt ttattattgc atacttaata
     5341 acagaactat agcaaaaaat cccgtgtatc aaaacgggat tagattaagt ttcctcaatc
     5401 actattcata acacaattat tataacaagt aagtaaacaa ttgaaaagga catttattga
     5461 aaacggttgt tttttactta aggaggaaat ttagtgaaaa aattagttgc attgtttttg
     5521 gtcgcaggaa gtttgttgag tgggtgtggt gctactacgt ctactactca aagcacaagt
     5581 tcagctcagg cttccagcag cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa
     5641 gaacaagaac aaattgaaaa gaaaaaagaa gaagagaaac aaaaagcgga aaaagctgca
     5701 ctagaaaaaa aagtgacaga agcggaatct gctttgtcac aagctgaagc aaatcctaca
     5761 aaagaaaact atgcaactgc ttcagcagct atccaagcgc tacctgaaaa caaacaagag
     5821 ttgacaagcc gtcttgctgc aattgattcc acaatcaaag caaaagaagc agaagaagaa
     5881 cggaaaaagc aagaagaaat tgcacgacaa caagcagaac aagcgcaaca agaagcaatc
     5941 gcacagcaac aagcagaaca ggcgcgacag caagcagcag ctgaacaggc ctctcagcag
     6001 gcacaagccg cagctcctga gcaaaataat gaacaaactg tctatgtcac accgaccggt
     6061 tcaaaatacc atacacataa atgtggaaat ggtacgtata cccctgccac tttaagcgaa
     6121 gctcaagctc gtggtttaac cccttgctcc aaatgttatg gaaactaatt caaaagttat
     6181 actataataa taaaattctt ctgaaacata aaaaaataga agctggcgtt ccagatagta
     6241 aaccaaaaat agcagtttac acgtccattt cgtcagcttc tctctttttt catttagtct
     6301 gttccctctg cttttttgga catcttcatt cattctttct caatcaaata gtacacctct
     6361 ttttcttttc gcagtttata tcttcctttt ttcaatttca ttgaaaactg tttcgaatgt
     6421 ttatgtttta catggatttt aagcaggagc tcctcatgtt gcaagcgata gttcctgcct
     6481 tttaaatcag aaaatctcac tagtccgtat tcatcagaag ctttcacaaa tggaactttt
     6541 tctctagtaa tatagtgttt gccgttgagc gtttttacag aaaagaccat tccttcgatt
     6601 ggtcgtccgt ttgtgttctt tacataaaaa gataaaggat agtttcgctt agcgatggcc
     6661 aaagaatcta aataagcgat gagtagccaa actaagccga ttgatccaag tagtataccg
     6721 ataaaccaaa taaaaaattt ccattttttc cattgatcaa ttccttggat cgcttttttt
     6781 gcctcttttg cttgataagg aatacgatgc cccgtcacaa gcagtcgatg tgtattgacc
     6841 atataaggag tacaggtaag caaagtcacg agatctctgc ctttttggat acccaaagaa
     6901 tctacttcat caggtaagac cacttggatt ttttctactt gatacgccaa tgtctttcca
     6961 ttgatctgga tgaaaaactg gtctcctttt ttcaacttag gcaaatctgt aaataatttt
     7021 gcttctggga gtccacgatg cccagaaaga acggcatggg tgtttttccc gccagtcgga
     7081 taagaacttt cttccaataa tgacgttcct ttttgcaaaa atatttccgt tgtctgatcg
     7141 aatataggta agctgacatt gatttttggg atttcgatga ctccaagcat atgttccata
     7201 aaaaaggcat tactaggtaa atcttttaat gcttttgcat caactgcttg gttgaaactg
     7261 cctatccctg gtgatacgtt ttgctctgcc aactgttggt ttttctttgt cattttttcc
     7321 tgctgttttt ttatttcctc tgaattctta ttcgatgctt gcttttgata atggatgatc
     7381 atttgctggg caagaaaatc attcagtgaa tcttctacaa aaggataaga aaagatcccg
     7441 atcccaacga ctaaaatgat tgccatgaag atgtccagta ttcttattct gcttttattc
     7501 atcatgtcca cctattttct aatatgtata tgaagaggtt gtg
//
LOCUS       DAZUMI010000131         7476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000131 DAZUMI010000000
VERSION     DAZUMI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.138"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            494..763
                     /gene="rpsN"
                     /locus_tag="ACG81C_002510"
     CDS             494..763
                     /gene="rpsN"
                     /locus_tag="ACG81C_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5065638.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            778..957
                     /gene="rpmF"
                     /locus_tag="ACG81C_002511"
     CDS             778..957
                     /gene="rpmF"
                     /locus_tag="ACG81C_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5065639.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            989..1366
                     /locus_tag="ACG81C_002512"
     CDS             989..1366
                     /locus_tag="ACG81C_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065640.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1387..1536
                     /gene="rpmG"
                     /locus_tag="ACG81C_002513"
     CDS             1387..1536
                     /gene="rpmG"
                     /locus_tag="ACG81C_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5065641.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1560..2543
                     /locus_tag="ACG81C_002514"
     CDS             1560..2543
                     /locus_tag="ACG81C_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5065642.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2573..2695
                     /locus_tag="ACG81C_002515"
     CDS             2573..2695
                     /locus_tag="ACG81C_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5065643.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2713..4236
                     /locus_tag="ACG81C_002516"
     CDS             2713..4236
                     /locus_tag="ACG81C_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5065644.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4260..4499
                     /locus_tag="ACG81C_002517"
     CDS             4260..4499
                     /locus_tag="ACG81C_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065645.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4692..5255)
                     /locus_tag="ACG81C_002518"
     CDS             complement(4692..5255)
                     /locus_tag="ACG81C_002518"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5065646.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5348..5566)
                     /locus_tag="ACG81C_002519"
     CDS             complement(5348..5566)
                     /locus_tag="ACG81C_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065647.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5755..6702
                     /locus_tag="ACG81C_002520"
     CDS             5755..6702
                     /locus_tag="ACG81C_002520"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5065648.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6669..6758
                     /locus_tag="ACG81C_002521"
                     /pseudo
     CDS             6669..6758
                     /locus_tag="ACG81C_002521"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6891..7298
                     /locus_tag="ACG81C_002522"
     CDS             6891..7298
                     /locus_tag="ACG81C_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5065649.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2650 a         1102 c         1454 g         2270 t
ORIGIN      
        1 tttgggatat agttataatt ctttttcaaa aagaataatt ttattaaaca gcatctaatt
       61 ttgaatacac gaaagaatca gtatgattga attagttcac ttaatgtcta ttcttataat
      121 ataaggaata atcaaagttt taaaaaattg gcgataaacc agttaaacat aaaagattga
      181 ataaaaagtt atagtttctt tcatattgcc gttttaagta gaggaaaagc tacttaaaac
      241 ggtatttgtg tttgattgga ctaataaaag tgaattctta tttttcagag cttaaactgc
      301 atattctttt aaatgatttg attatctcta tgtaccgata aattcgtttt caaatagtta
      361 tctattcata tgagttttat ttcatgttat ttattgttga caagagcaac tcttgttggt
      421 aaagtatcag tgtcaaaacg taacgattac gatttgagat gtctaaatca aaataaaagg
      481 agtataaagg tatatggcaa aaaagtcgaa aattgctaaa gcaaagaaac aaatggcgat
      541 gattgaaaag tacgcagata aacgtcagga attaaaagcc gcaggagatc gcaccgctct
      601 cgctaaattg ccaagagatt caaatccgaa tcgtctgagg cttcgtgatc agacggatgg
      661 tcgtccaaga gggtatatgc gaaagttcgg tatgtcccgt attaagttta gagaacttgc
      721 ccatcaaggg ttgattcctg gagtaaaaaa agcaagttgg taaaaggagg aaatatcatg
      781 gcagtaccag caagaaaaac atctaaggcg aagaaacggt taagaagaac tcatcaaact
      841 gtagtaaaac cagaaatttc atttgacgaa gcgaatggtg attatagacg tagccatcat
      901 gtatctttaa aagggtacta taatggaaag aaagtaatta aaggagcttc aaaatagttt
      961 aatatataac ttttaggagg gaatcaaaat ggaaattatt tacccacctt tagttgaaga
     1021 aggattaaag tattatcttg aaacgaccca gcaatcgcta gataaatgca ctttttatcg
     1081 ttcgatggta gagagaggaa ttattactga aacgggttta ccgacccaac aggcaattga
     1141 aaatggatta gtcaaagatt actatgagga ccaaggctta tcatttgatg attttttaag
     1201 aatctatcca atttttgaag agtatgacga ggagttattt caatgtattg atggttattg
     1261 ggagatacct gttgatatga aagaaaattt agtttcacaa ttggaatctg gcgagttaac
     1321 ttttgaagat gcacaacaaa ttcaagcata tcttgaagat cgataagtat tgaaggagga
     1381 agatttatgc gtcaaaatat tattttggaa tgtgtcgaaa ctggagaacg tctgtatctc
     1441 accagcaaaa ataaacggaa taaccctgaa cggttagaat taaaaaagta ttcgccaaaa
     1501 ttgcgcagac gggcaatttt caaagaagtg aaatagctaa gtggtaaaaa ggtgacaaaa
     1561 tgggcatacc agttacaatc gtttctgggt ttttaggggc tggaaaaaca acgctaatca
     1621 atcaagcctt gcaagtctca ccctacccca gggaagaaat tattataatt gaaaatgagt
     1681 ttggtgaagt tgggattgat cacaaattat tagttcatag cgaggaacgg gtgttacaac
     1741 taaataatgg atgtatgtgt tgcagtcttc gcagtgattt attagcaata ttgatttctt
     1801 tactggaaac aatagaagaa catcaacaac caatttcaca aatcatcatt gaaactacag
     1861 gtatagcaga tccccaacca atcattcaaa cacttttaac agcgcctcaa ctaagaggaa
     1921 gagtatatat cgatagttta ttgactgtta ttgatagtga cagttttgaa cttattgaaa
     1981 gagaaccaca agcccttaag caacttgtca tagcagatcg catttttgtg tctagaaaac
     2041 aaaacttgaa tttatctgca ctaaaaaaat tacagagaga aatcaaaagt attaaccctt
     2101 tatccgatat tcgaatgttc acacatagtg aaacgattca aaaaaaggat ttttataatt
     2161 tggaaaaatt caatcatccc ttagtttttg aaagtgattc atcagaaaat cactataatg
     2221 gcaaacacta tcatcaacat catcaagaac atgagtttaa atcaattctc ttaacttcag
     2281 atcaagacat aaaaaaagat tttttattcc aattaattga ccggctgatt ttaaccaacc
     2341 aagggagcct gtatcgtttt aaaggattga ttgctttgca agatcaagat tttatagttg
     2401 ccatacaagg ggtaaacgaa caggttaatt ttcaatacac cactcgacaa aaagaccaaa
     2461 ccactattat tctaattgga aaaatgttgg atgaagaaag gatcaaacag agttttgata
     2521 aattactggc ggaaagcaaa taatgataag tattgattag gggtaatttc taatggagaa
     2581 aacggatata tctagtgcat atcgacgact taaaagcccg aatatcaaaa ctagaaaaag
     2641 ggcattaaaa attatcaaag aacataaaca aaataaaatg aagaaattag cataattaag
     2701 gaaggaaaaa gaatgaagaa aatcagtgta ggacttattg ggatagcagc tctaggatta
     2761 ttgggagctt gctccagcac taatgatgca aaagtatcga atgataaaga tggaaaatta
     2821 gaaattgtaa caacattcta tccaatgtat gatttcacca aaaacatcgt cggcgatgaa
     2881 gcgaacgtgg atttaatggt tccagcggga tcagaacctc atgactatga gccctcagct
     2941 aaggatatgg caaaagctca tgatgctgat gtttttgtat atcataacga aaacatggaa
     3001 tcatgggtac caaaagctaa agaaagttgg aagaaagctg ggcctaatgt agtagaagga
     3061 acaaaagaca tgattttatt gccaggtagt gaagaagaag atcatgatca cggagaagaa
     3121 gatcaccatc atgaactaga tcctcatacg tgggtttcac ccaaaatggc aattaaggaa
     3181 gtttcaaata tcaaggatca gttagttaaa ttgtatccga aaaaagcaaa agtatttgag
     3241 acaaatgctg aaaagtattt gactaaactg aaacggttgg atgctgacta taccactagt
     3301 cttaaagaag cgaaacaaaa aagttttgtg acacaacatg cagcctttgg atatttagct
     3361 ttggattatg gattgataca agttcctatt gctggactaa gtcctgaaga agagccatct
     3421 tctggtcgat tagctgaatt aaaagagtat gttaaaaaaa ataagatcaa ttatatttac
     3481 tttgaaaaga atgctaatga taaaatcgca aaaactttgg ccaatgaagc tgggataaaa
     3541 ttggaagtat tgaatcctct tgaaagtctt acaaaagaac aaatggataa tggtgaagat
     3601 tatgtttcag taatggaaga caatctaaaa gcgttagaga aaacaactat ggttgctggt
     3661 gaagaggtag ttccagaaaa agaagcgaag gatgaaaaga cagttgctaa tgggtacttt
     3721 aaagatgccg atgtgaagga tcgcgaattg agtgattata caggcgaatg gcaatctgtt
     3781 tatccgttgt taaaagatgg aattttggat gaagttttcg attacaaagc aaaactcaat
     3841 aaagacatga cagctgccga gtacaaagat tattatacta ctggctataa gacagatatt
     3901 gatactatca acattaaaga caacaccatt gattttgtag tcaacggtga acatcatcaa
     3961 tacacataca aatatgttgg atacaaaatt ttgaattatg aaaaaggaaa ccgtggtgtt
     4021 cgttttaatt ttgaaactga tgatgcaggt gctggtcgtt ttaaatatat tcaatttagc
     4081 gatcatggaa ttgcaccaag taaggcagag catttccata ttttctttgg tggagaaagt
     4141 caagaaaaac tttataatga aatgcataat tggcctactt tctatccagc ttcattatct
     4201 gagcatgaga ttgctcaaga aatgatggcc cattaaaatc caatctttgg aggaaataca
     4261 tggtaagaaa agtattattg tctatggatt ccaagaatat tgatgcttat cggcgttatc
     4321 aatttgcaaa gcaaatgaga ttaaatggga gtacattagc tgatattgct gcaactttac
     4381 atgttagtat tgacacagtt catagagtat taaaacggga tcctgattgt tgttttatca
     4441 aggtcaatac tggaagaagt agacgtttag caaaacggca taatagcttg gccaattaat
     4501 tgataatacc agagtagatc atttttccct tatttggatt tgatctgctt tttattattt
     4561 cttgaattgt caaatcaagc gcaacacttt atcaaagaag acttcataag gtgttctcca
     4621 atctaaacac tttctaggtc tgttattcag tttttcaatc atctgattta tcgttgagtt
     4681 atctaccgta gctatctctt tcgattttgg caagtattct cgaatgagtc tattcgtatt
     4741 ttaatttgtt cattgttgcc aagggcatga ggatctggaa aataaaaggg catattattc
     4801 aatacttctg taactgcgaa atgattggag aattcttttc ctctatctgg agtgatcgtt
     4861 ttgaccgcct catttggaat actgcttaaa agttcaatca ttttctcgct cacataagcg
     4921 gctttctttc tggggacctt ctttcctgct aaaaggaagc atgattttct gtctgtcaaa
     4981 gtcactaaac aagtaccact agttttgcca gctacaatat cggctttcca atgaccaaaa
     5041 tcacttctat catctgcgga ttttggatgg ccatggatcg aatgagtaag gcaaatcttc
     5101 ccacgagact cgacatgatt ttttgtatgt cttgtttttc ctcgatggtg caatgaacgg
     5161 atcaggcctt tattgccgtg tgagaggggt tctgtatcga aaagatcatt gcaaattgca
     5221 cggtagatcg tgataaaact aattgaaaag tccatatgtt catgcttcaa acgattcgag
     5281 attttctctg gcgaccactg aaaatcgaca aataaatgac gaacaattga ccaaactcac
     5341 ggatcattta gaatgtgata acgtccgcat ttttttcttc gataagcata atttttttcc
     5401 gctttggaag gtgattagtt tttttcttgt ttcttctcag ttcatgtaaa atagtaaacg
     5461 ggctatgctg aatgtcgttg ccaattatac taatagaaaa tccttcttcg tgaagcagaa
     5521 agattctttc tcgtttgagt atagtaagat gacaatactg gctcataatt attttccttc
     5581 taggagtgtt ctcgacaaac ttatactagt tggatatcga ttatgactca tttttatgtg
     5641 ttgcacttaa agtgtaaatt caaggtatag aaagaggttc cctatgaaaa aatacctatt
     5701 acaatcttaa ctggctatct tggttcagga aaaacgatac ttctaaatca tattctgtac
     5761 ggtgatcacg gaaaggaaat tgctgtcatt gtaaatgaca taggggaagt aaatgttgat
     5821 ggggtattag tcgacaagaa aggttttaaa agaactggag aaaaacttgt ttcgatgccg
     5881 aatggatgca tttgttgtac attacgcgaa gatttgattg ttgaactaga tcaattagtg
     5941 caattttctg acttgaatca aattgttatt gaagcttcag gaatcagcaa accaattcca
     6001 attattcaag caattatatt agctaaaaac cctttagaca ttgatttgac ccaacgatta
     6061 gagattggtg ccattattat agtagtggat gtaaaacgtt tttgggataa tttcgaaacg
     6121 ggtgaaaaga ttcaggaaag aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt
     6181 cgtggcttgt taattgatca attcgagtgt tgtaacatat tacttttaaa taatagtgac
     6241 ttagtgtcaa ccaagacgat ttacaaaatt aacggtctaa ttgctaagtt acagtccaat
     6301 gccaaaataa ttaggacaat taaaggtgaa gtagacttat cgaaaattct aaatacaaat
     6361 ctttttaata tagatgagac ttcctattca atgggctggt tcaaagagtt agagcttggc
     6421 tatcaaaatc atattcctga aacagaagaa tatggtatct caagttttat ctatcgaaat
     6481 cggttactcc ttgacgcaca aaaatttgcc catcttacgg ataaaaattt tcctggcaat
     6541 attacaagag caaaagggtt tctttggttt gcccaacata gtgatttttc gacgatcttt
     6601 gaaacatcgg gtagccataa aagtattgaa tcattaaatt attgggtagc tagtttacct
     6661 aaaattgagt gtcaaatgat tatacaaaaa aacccgaatt gaaggaagcc tggcatccgt
     6721 tgtttggtga acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta
     6781 ttaaggaaca acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa
     6841 tttcaccatt tccatggaaa aattctaata tgtttagcca gatatcgtta atgaaatttt
     6901 taagaaaagc tggtgtcaag ataggcgatg tcataattat tggcattttg attattccct
     6961 cttttattcc cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta
     7021 tcaaatatgc catcgttcaa attgatggca aggaaatgga tcgcttcgat ttggataaga
     7081 tcaatcataa actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta
     7141 aagatggaag aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg
     7201 gatggatctc aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta
     7261 ttgagaaaaa agaaagcaaa gattactaca tttactaatc tggaaataga tgaggaggtt
     7321 tttaataaga aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc
     7381 gcttgtggac aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc
     7441 aatggtttag ttaacagtcg gttagattac ttattt
//
LOCUS       DAZUMI010000132         7324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000132 DAZUMI010000000
VERSION     DAZUMI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.139"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..2765
                     /gene="clpB"
                     /locus_tag="ACG81C_002523"
     CDS             156..2765
                     /gene="clpB"
                     /locus_tag="ACG81C_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5065650.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2916..3602)
                     /locus_tag="ACG81C_002524"
     CDS             complement(2916..3602)
                     /locus_tag="ACG81C_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065651.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3580..4074)
                     /locus_tag="ACG81C_002525"
     CDS             complement(3580..4074)
                     /locus_tag="ACG81C_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5065652.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4094..5314)
                     /locus_tag="ACG81C_002526"
     CDS             complement(4094..5314)
                     /locus_tag="ACG81C_002526"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5065653.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5461..7296
                     /gene="lepA"
                     /locus_tag="ACG81C_002527"
     CDS             5461..7296
                     /gene="lepA"
                     /locus_tag="ACG81C_002527"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5065654.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
BASE COUNT         2423 a         1391 c         1491 g         2019 t
ORIGIN      
        1 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
       61 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
      121 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
      181 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
      241 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
      301 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
      361 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
      421 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
      481 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
      541 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
      601 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
      661 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
      721 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
      781 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
      841 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
      901 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
      961 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
     1021 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
     1081 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
     1141 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
     1201 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
     1261 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
     1321 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
     1381 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
     1441 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
     1501 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
     1561 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
     1621 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
     1681 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
     1741 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
     1801 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
     1861 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
     1921 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
     1981 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
     2041 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
     2101 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
     2161 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
     2221 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
     2281 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
     2341 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
     2401 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
     2461 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
     2521 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
     2581 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
     2641 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
     2701 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
     2761 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
     2821 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
     2881 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
     2941 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
     3001 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
     3061 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
     3121 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
     3181 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
     3241 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
     3301 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
     3361 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
     3421 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
     3481 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
     3541 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
     3601 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
     3661 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
     3721 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
     3781 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
     3841 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
     3901 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
     3961 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
     4021 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
     4081 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
     4141 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
     4201 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
     4261 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
     4321 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
     4381 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
     4441 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
     4501 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
     4561 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
     4621 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
     4681 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
     4741 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
     4801 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
     4861 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
     4921 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
     4981 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
     5041 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
     5101 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
     5161 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
     5221 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
     5281 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
     5341 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
     5401 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
     5461 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
     5521 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
     5581 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
     5641 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
     5701 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
     5761 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
     5821 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
     5881 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
     5941 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
     6001 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
     6061 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
     6121 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
     6181 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
     6241 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
     6301 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
     6361 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
     6421 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
     6481 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
     6541 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
     6601 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
     6661 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
     6721 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
     6781 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
     6841 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
     6901 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
     6961 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
     7021 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
     7081 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
     7141 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
     7201 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
     7261 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
     7321 taaa
//
LOCUS       DAZUMI010000133         7145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000133 DAZUMI010000000
VERSION     DAZUMI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.140"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..613
                     /locus_tag="ACG81C_002528"
     CDS             <1..613
                     /locus_tag="ACG81C_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5065655.1"
                     /translation="GEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEE
                     EFGHLEGDTIVGIHHKSAIITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNL
                     FKSITFDCGKEFSNWKSISNTNDIDIYFANPGTPSQRGLNEHSNGLLRKNGLPKEMDF
                     NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(681..1454)
                     /locus_tag="ACG81C_002529"
                     /pseudo
     CDS             complement(681..1454)
                     /locus_tag="ACG81C_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1490..2677
                     /locus_tag="ACG81C_002530"
     CDS             1490..2677
                     /locus_tag="ACG81C_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFS5065656.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            3410..4378
                     /locus_tag="ACG81C_002531"
     CDS             3410..4378
                     /locus_tag="ACG81C_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5065657.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            4446..5204
                     /locus_tag="ACG81C_002532"
     CDS             4446..5204
                     /locus_tag="ACG81C_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS5065658.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5272..5982
                     /locus_tag="ACG81C_002533"
     CDS             5272..5982
                     /locus_tag="ACG81C_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5065659.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6018..>7145
                     /locus_tag="ACG81C_002534"
     CDS             6018..>7145
                     /locus_tag="ACG81C_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5065660.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2432 a         1115 c         1439 g         2159 t
ORIGIN      
        1 gggagaattc aatgttttac atttaccgat gcaaggaaaa cgaaaaccaa atggttataa
       61 ggaaaaacgt ggaaaacagg cattcaaaag aaatatttct gaacgtaaaa aagattatgt
      121 cgttttcgaa gaagaatttg gacatttaga gggtgatacg attgtcggca tccaccataa
      181 aagtgccatc atcacactcg ttgagcgact ttcaaaagcc attatcgcct tgaaaccaga
      241 aggccgtaag gcagttgata ttgaaaattc gattaatgaa tggcttcaat ccgtacccaa
      301 aaatcttttc aaatcaatta cctttgattg tggaaaagaa ttctctaatt ggaaaagtat
      361 tagtaatacg aatgatattg atatttattt cgcaaaccca ggaacgcctt cccaacgggg
      421 attaaatgaa cattcaaacg gactcctccg aaaaaatgga ctaccaaaag aaatggactt
      481 caaccaagtc aatcaaggat tcatctcatc cgttgcgtcc aaaagaaacc atatccctag
      541 aaaatcacta aattaccaaa caccattaga agtttttttg agttacgtaa atggaaagtt
      601 ttgtctcgct taatttgaca aataataagc caataaaact tagtccgata tgcggcgcgt
      661 tctgtgcgcc gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt
      721 gcccgaaagt tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg
      781 ttatacagcc cctcagtgac gccgtttgag tgggacacct taaaggcatt tgaaataccc
      841 gattggtagc gatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa
      901 attgttttaa gggtttcttg aaagcctacc gtatctcgtt taacgaaata atagcgtaat
      961 aattgacaaa aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcattaacg
     1021 atttctgttt gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtgcctcc
     1081 catgtcgtgg aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc
     1141 tgattttcag agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag
     1201 ctgcgattga tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag
     1261 accgttttaa gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt
     1321 aaacgggcct ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct
     1381 tcaagggtcg taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca
     1441 gaagtggcaa cattgtgtac ttttctttgc aatcttgtgt acaaaactat tgcaaaacac
     1501 agcgcataag aatccttaca aaacagtaga tgaagcaacc caattagatc ttgttatagt
     1561 tgttaatcag ctcttaacgg gttacgattc aaaatgggtc aacaccctat acttagataa
     1621 gtcacttgaa tatcagaaca taattcaggc tttttcacgc acaaatcgtc ttaatggtcc
     1681 tgaaaaaccg ttcggaacca ttcgttatta tcgtaaagca catacgatgg aacagaacat
     1741 tgaagaggct attgagttat actcaggcaa acaagcaaaa gggttatttg ttgaaaaatt
     1801 atataataac ttagaaaagt tgaacgatat attcgaagaa attaagtccg tatttaatat
     1861 ttctggcata gaaaacttca gtcatttacc ggaggttcca ggggaaatta agcaatttgt
     1921 aaatctttat aatcaactaa tccattgtta tgaatcagcg atgatacagg gattcacatg
     1981 ggcaaaatcg acttatgaaa tccaggatta tgaacctata acagtagtaa tcacagaata
     2041 tgaatttaat atcttggcac aacgttacag cgagatagag agagaaccta gagacggaaa
     2101 tgatagtata gcgtatgaca ttgattacca attacctgaa ggtagtcgta gtcgtattaa
     2161 ttatgagtat ttaaacacaa acttcaaaaa atacatgaat ataaaagaag aacgtgattc
     2221 gaatttaatt caatcattgt taaacgactt acatcgcaca tttgcgcaat taccagtgga
     2281 gttacagcca tatgctgaga tgattctaaa tgatgtaaat aatggggaat tagttatcaa
     2341 agaaggatgg gaatttactg attacctcaa tgaatatcag cttagtgaag aggacgattt
     2401 tatccaagat ttagtggatt caaccaatat taatgaggca ttattacgtg agatgttgga
     2461 tttacgctta accgaggtaa acattaatga atatagtcga tttgataaat taaaatcaag
     2521 tgttaacgta ggtcatttca gtggatatat cgagaaaaat ttaggtaaaa tggtaatacc
     2581 aatcaaggta aatatgctaa ttgacagatt attgcgtgca tttttattag aagatgtatt
     2641 cgatgttaca gagtacataa agaattactt taactagagt acacctacat cttatagtac
     2701 ataaaaaaat tcatcttttt tagtttaata attcaccaaa aaggtgagta atgccatttt
     2761 tgaaaattga gcttgtttta tgtctacatc tgtatccgta tataatccaa aggattataa
     2821 actcagaaga gtctttattt tttctgaata taactactgt atcaaaaaca atcgattatg
     2881 agacaaaaca aaatgacctc ttttatttaa gaaaggaggt cattttttgt atcataaatt
     2941 gtattaaaat caatgtatca ctttttaatc aattttaaga ccataaattg cgtactaaaa
     3001 gaaattctgc aattcaaaat aaagcatttt aagccatttt caaatttaag tgcacccatc
     3061 tacccgtcaa atcactttat tttccactcc agtagtttcc taattcaact gtgtgcaaaa
     3121 cgatttcttc taaacaaatc aagacaaact tataaacctt catcccagca ccataattat
     3181 ttaaatcata ggcatttttc gaaattagta tatgtactca agtttcactg cagattattt
     3241 atatttttta gagaaataaa tttttcctat tgacaaacac aaagaaagcg gatacaatac
     3301 aattatgtca catgacgtta tatgacgtta tgtaatatct ttacatttaa tcagttactt
     3361 ttattttgga taaaattaac atttttaaaa ttttaaggag gaaatgttga tggatgtagc
     3421 aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag atttctggaa tttatttggt
     3481 ggcttgtggc ggttcgctgg tagatatgta tgtctcagat tattttctta aagccgaagc
     3541 atcgaagtgt attacaggct tatatacagc gaacgaattt aaccatgtaa ccccaaagca
     3601 attagatgat aaatctgttg tcattctatg ttctcatgga ggaaatacag cagaaacggt
     3661 aaaagcagga gaagtcgctc aagctagagg agctgtgact gttggtctga ctcataataa
     3721 aactgcggat ttattaaagg tttctgatca tagtttcctt tacgaatggg gagatgatgc
     3781 aaaaatagtc aataatccta tggccatcat tcttgattta actgtatctt tggtcaatgt
     3841 taccgaaaca tttgaacatt acggtaaatt cagagaaggc atgaaggtta tcgataccgt
     3901 aattgaaaat ggcattaagc aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa
     3961 agatgaaaca atgttttata tcttaggtag tggaccatca tatggacatg cttatggttt
     4021 ttcaatttgc tctttgatgg aaatgcaatg gcttgattca acttctgttc attctggaga
     4081 attcttccat ggaccttttg aagtaacaga caaagatacg aattttattc tcttgatgag
     4141 cattggacgc actcgtgctt tagatgaacg ggcaaaacga ttccttgata aatatgcaga
     4201 aagagtagaa atcgttgatg caaaggaact aggattggat atgatatcag atgaagtttc
     4261 tgaattcttc aatcccattt tattctacag tattctttca gaatatcgtt cagctttagc
     4321 agatgcacgc aatcatgatt tagatgttcg tcgctatatg gggaaagtgg agtactagga
     4381 ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg
     4441 gaattatgta tcctggagga aattcattga attttgcagt ttttgcaaaa aaactgggac
     4501 atgaaagtag ctttatggga gtttttggct cagatgagga ggcagctcat gttttatcag
     4561 caataaaaaa aataggactg gacaacagtc actctcgctt tgaaaaaggc gagaatggct
     4621 gtgccagagt gaagattgat gagggcgacc ggattttcct tggcagcaat tcgggaggtg
     4681 ttactcgtga atacccgatt cagcttactg aagaagacag agaatattta aaccagtttg
     4741 aattgattca tatgggatta tatagccatg tcaatcatct attagaagag ttacaaaatg
     4801 tttctggaaa gatttcttat gatttttctg acgactttac ggaagaagag gttcagcggg
     4861 caattgataa agttgatttt ggcttctttt ctatagaaga cttttccgat gaagaagtaa
     4921 agcagttttt gatgaagcat ttttgtgagc gaaatgatgt attgattgca acccgaggag
     4981 aaaaaacggc tattggttat gatggagcaa agttttatga agtgaacccc gtgctagaag
     5041 aaccagttga tacaatggca gcgggagatt ctttcttaac agctttccta ctttattttc
     5101 tcgaagggga cgatatagtc accgcgatga aaaaggggaa tgaatttgct gggaagtctt
     5161 gtctggttga cggttcgttc ggctttggta ttcattacga ttgagcttgt tttaaattcc
     5221 tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat
     5281 acaaatgcac aattaccatt gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat
     5341 acaggggagt atcccgagaa tacgaaaatt cccgctgaat cagagctgat agaaagttat
     5401 gaggtcagcc gaatcacagt gagaaaagca gtagaagaac tggtagaaga agggtaccta
     5461 cttaaaaaac aagggaaagg aacttttgtc aaccggcata aagtttttcg taaaattgaa
     5521 tatgttatgg gtttcagtga gagttgtaaa tctaatggtt ttacaccgac tagcattgtt
     5581 ttggaacgca agatcattcc agcaactgaa gggatggcaa aaaaactcga tatcgctcaa
     5641 ggtgatgatg ttttatatat ccagagaaag cgaatggcgg ataacatccc cattatgtta
     5701 gaaaacaatt atttttctta tgaaagatat agtttcttaa tggaggaaga gctgactgat
     5761 tctttgtatg aagtacttaa agtccaaggt accttagcca tcaatcctgg ggaaactgca
     5821 cttgaaattg tcacagctga tgaccaaatt gccaaaataa tggaagtagc tgttgggacg
     5881 ccctttttct ttatggatac tttaatcaac gatcaaaaca acataccaat tcatgtaggt
     5941 aatcaatatt acctgggaga agtatatcgt ttttacttat gatttcctgt tttgatttca
     6001 aggagtagat tttatttatg aagcaagtat tgcggtcaaa aatgattttt gatggagagc
     6061 agccaccttt caaaggttat attgtaatca atgaagggat cattgaaaag gtggaaaaca
     6121 actggaatta cagtgatcta ttaaatgaaa acacggaatt aattgcttat gatgatcaat
     6181 tcgttatgcc agcaattcat gataatcatg tatttttcag tggttatttg gctatgaatg
     6241 ctggcataga tttaagcgat acatcttcag ctaacgaggc tgtagagcgt attaaagctg
     6301 taatgaacca taaaaaagca gatgaaccaa tttatgctca tggctgggat gctgccctgt
     6361 ggaaaactga cccatcagaa gagctattga atgaaataaa ccactttggt ccaatcacag
     6421 cgatcgacaa aaatcgcagc cattgttgga tgaatgctat tgctaagcag acttatggtt
     6481 ttacagaaaa agaaaccagt gctgaaagtc gagtattatt aattaaggaa atgctagcaa
     6541 acaaaaagct tttagcagag gtttacgctg aatttgaagc attactgctt tcaaaaggtg
     6601 ttatatcaat gaaagaaatc gtttttgatg acgcagaatt tctttctaat atacctcaga
     6661 ggaaaatact tagtaaccta tatgtgcagg cagtccaaga acctctacag catgaacttc
     6721 tatatgagta taaggaaaaa gttttccctg aaaaggtccg ctttggtgga gtgaagatca
     6781 tggtagatgg tgttgttgcg gatagaagtg gagatattta cggtgtttat cagtcaggag
     6841 tagcttcacc cgagattgac tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag
     6901 tagagattcc ttgctgtttg acaacggaag gagataaagc gggttataaa gctgcgtcaa
     6961 ttcttgtcga atttggaaag cggttacctg aaggcgtttt taattcgatt agtgatttgg
     7021 aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat
     7081 atccacaaat tctcggcttg aatcaaacgt tagaagaggc ttacatgtca actgttataa
     7141 aaaat
//
LOCUS       DAZUMI010000134         7092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000134 DAZUMI010000000
VERSION     DAZUMI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.141"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2669
                     /locus_tag="ACG81C_002535"
     CDS             <1..2669
                     /locus_tag="ACG81C_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS5065661.1"
                     /translation="NHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTADSQA
                     IINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSA
                     TDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNF
                     LQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNID
                     RSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGD
                     HKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLP
                     KPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVIT
                     LVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIF
                     FEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLG
                     TNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTT
                     VLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNC
                     QLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSS
                     NTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYSGAL
                     LIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSD
                     NKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAE
                     KGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2851..3090
                     /locus_tag="ACG81C_002536"
     CDS             2851..3090
                     /locus_tag="ACG81C_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065662.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3106..4479
                     /locus_tag="ACG81C_002537"
     CDS             3106..4479
                     /locus_tag="ACG81C_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065663.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4483..6120
                     /locus_tag="ACG81C_002538"
     CDS             4483..6120
                     /locus_tag="ACG81C_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065664.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6283..>7092
                     /locus_tag="ACG81C_002539"
     CDS             6283..>7092
                     /locus_tag="ACG81C_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065665.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTML"
BASE COUNT         2487 a         1147 c         1402 g         2056 t
ORIGIN      
        1 tgaatcacac ttcaattcag aagcatttac aactgttaaa tacactgggg atttcttatg
       61 ttaatttgag aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg
      121 cagattctca agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa
      181 acataactta taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag
      241 acaatgatat tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg
      301 gaagtgcaac agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt
      361 atttgaaaga aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata
      421 tttttcttcc cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt
      481 ttcttcaatt acttcaccga agatttggag tagaagatga agttgatatt gatacttctg
      541 tttataatcc ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca
      601 caaaagagcg tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa
      661 gtaattgttt taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta
      721 gagtaaaaag ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac
      781 aatggttagc tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag
      841 ttaccttact tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt
      901 cttgtttgtc tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata
      961 aaaataaaga tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt
     1021 ctgtagaatc attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag
     1081 aaggagtatt tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta
     1141 agcaaatacg caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg
     1201 atcattgttg ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac
     1261 aattagcaaa acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt
     1321 tacagattca acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa
     1381 aagaaaatga agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg
     1441 tattaactaa aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg
     1501 cattggttca taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg
     1561 cttctattgt gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa
     1621 ttaacctaca gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt
     1681 tatttggaaa tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa
     1741 agctagcatc aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa
     1801 ctcaaaataa ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat
     1861 tagattctaa ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt
     1921 ctgaagagcg tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata
     1981 tcgaattttc tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc
     2041 tttcttctaa tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag
     2101 ggattgcaaa aattgaagca acttttgtcg aagctcaaaa aaaaatatgg gctgttttac
     2161 ctgaacattc tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagctc
     2221 tcctaattca agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta
     2281 ttaattcgta cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg
     2341 accgagtgaa aacgttgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca
     2401 aacatacgcc cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg
     2461 taaatatttt tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc
     2521 taacctatct ttttagacaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa
     2581 gaacgaaacg tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt
     2641 cttcactcaa atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc
     2701 accatgttat ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg
     2761 atgacgagca aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt
     2821 caatcaaaca tttaagaaag gggaattcta atgactaaaa ataaaatgac actaaaagca
     2881 gaggttcttc tgtatattca agaacatttt tctaatcaag ccttttttac aaaaccaatt
     2941 tacttggctt tcgaaataag aggggtatct gctggatcta ttggaggtac tcttcaagcg
     3001 ttgaagaacg aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tggaagagtc
     3061 gcgaaagaat ggtatttagt tcattcatag agaggtgatt caagcatgcg tgtggcttgt
     3121 tatgatcgaa cgtctgttta tgaagaagcg aaaatcagta atgacgctca atatgatgca
     3181 gtgaaagagt atgttttaga gaaatatgaa caaattcctc aacgattttc agacaaagga
     3241 gaatcaggtg gaaaagcgga tcgtccagct tttcgacagc ttagagcttg ggtttgtgcg
     3301 tcagaagaag agggggtgat ctatgtctgg cgttatgatc gattggcacg tcaagccaaa
     3361 atagccttag aatttattga gttgtgccaa caacatcagg ttcaagttta ttcgatttct
     3421 gaacctcttc ctgcttcttt ctcgcacgcg tcaacgaata aactatttgt gcaattattg
     3481 ttcacttttg cagaacaaca acgaaacgtc attattgaaa atgttcgtgc cggacttgcc
     3541 tataaaaagc aacaacggca atatttgtct tctgcggttc catatgggta tcgtttagtg
     3601 aatgggaaaa tccaacaaga agcacatgaa gcaaaagttg tccgccatat ctttcaactt
     3661 tatttgacta aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagttc
     3721 ttcttccgtg aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg
     3781 tactatggcg aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt
     3841 ctctcgaaaa caatcttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa
     3901 aaagatacgt atccgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat
     3961 ctgagttcga agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt
     4021 accgaccgaa gctgccaagg aatctttctt cgggcagaaa gggttgagtc agaagtattg
     4081 gaaaagatac aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaattg
     4141 acgcaacaat tagccgaaaa gcgaaaagat gaaaaacaca aagtaatcca acaaaagaaa
     4201 caagaagcac aatggctcaa ggcatttgaa gagggcaatt gttcactgga agaactcaag
     4261 caacagctgc aagctcttca aaagccgagg tctacttctt cgtttctttc actccaacag
     4321 tgtgaagcaa aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattgtta
     4381 tgtgaccatg tttgtcggat cgaggtaaca aaggaaaaaa cagtctgtgg gatttacttc
     4441 cgacaaatga cacaaaatat tttagaaggg aacgaatgat ctatggaaac gacaccaacc
     4501 aaacgagtcg cattgtatat tcgggtgagt acccaagaac aagctttaga aggacatagt
     4561 attcaagcac aagaacaaaa tggacgacag tttgccgaac ggatgagcta tgaagtggta
     4621 gaagtttatg ccgatgaagg gattagtggg aaatccacaa aaaatcggtt cgcctatcaa
     4681 cgaatgatga acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc
     4741 cgtctcggac gcaatatgtt agatgtctta caaaccgtcg aggaactgtt gcgatatgat
     4801 gtggggttac attcgatctc ggaacaattt gacgtgacta cttcgacagg aaaactaatg
     4861 ttacaactcc ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcag
     4921 atgtcaatgc aaagcttagt gaaagataaa aagcgttatg caggtggacg acgtttaggc
     4981 tatgtcagtg gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg
     5041 aaaattgttc gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc
     5101 aattttttga atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca
     5161 gtcaaagata tcttacgcaa taaaatttat gcagggtatt tagaatatgc acgctatatc
     5221 aattgggaaa ccaaacgacg aaagggcaag aacccaaaac cgattttagt gaaaggtacc
     5281 catgaaccga ttatttcgga agaagattat caaaaagtgc aagaacgcct tgttttagaa
     5341 ggcaaacagc ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt
     5401 ccagaatgtg gggcaccgat ggcagcgagt aatgtcacaa atactctaaa ggatggcaca
     5461 aagaaacgaa ttcggtatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt
     5521 catgccaatt cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag
     5581 atcgttcagc taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg
     5641 cgacaaagcc aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg
     5701 aagactaaaa ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg
     5761 ttaaaagacc gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat
     5821 gaaatcttag ggatctttca acaacaagga gagccgattc aagtcaaaga tgtccaacgg
     5881 atattgacta gtttggatcg ttttcttgcc cagagtgaga agaagcaagt caaagccttg
     5941 tatcgtacct ttattgagaa aataaccttt gatcctaact ataaggagat attggagatt
     6001 acaatgaaat tcacgcctgc cgtggtacaa caattgaatg agcaatatca aatagccgta
     6061 tccaaaacaa aggatacggc tatttttgtg ttaaagacac cctttacgtt aacgatctag
     6121 tctaggttat atcaaacaac gttagagggg cgctaacgct tctctagcga tttgaaatcg
     6181 ttagatgagt cgttagggag gtccttatca taaataggga tatttattaa tgaatatttg
     6241 aaatataatt gtatataaga attaaaaaga gtggggatag atatgaaaaa gatgggtaag
     6301 tgtattttat gtgaaaaata cactgaactt actaaagaac atgtaccacc taaaaaatca
     6361 gggaatactg gaggtaaaaa gaccagaact ggtaatttag atgatttcct taagagcgat
     6421 tttacaaaag gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa tgtttattat
     6481 acgttatgta gtaaatgtaa tagttttttt ggtagtgagt atgttgagga atatattaga
     6541 tttgcagaag acaataaaaa tttcctttat aacaatacaa gtttaaagaa tggtaagtct
     6601 gatttaacac attcaataaa gaaaatgaat tcacttagag ttgcaaaagc aattgtagca
     6661 atgtttttta gtttaaatgg ggatgaagat tcaatggata agccattctt agattcggta
     6721 cgagaatatc ttagcaatcc taaaagtact ttatttcctc atgaagacta caaaattata
     6781 atgaattatt atcttccaat aggtgctcca cagaattcta aaatcaaaat tcctaatgac
     6841 attgtaaatg atggtgacac tcacaatgta ataaaaggtt ttatgcccct tgatctatca
     6901 actgggagaa aaattcgatt ttcagaaatt cagtatgaag gaattggttt tactcttata
     6961 gatttaaaaa attcaacttt tgaaataaaa gaaggattag atttaaaaaa tctcctttgt
     7021 gcagatgata aaatcaaaaa aatacaattg tacaaagtcc ctatattaac accaacagta
     7081 cttacaatgt tg
//
LOCUS       DAZUMI010000135         6644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000135 DAZUMI010000000
VERSION     DAZUMI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.142"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG81C_002540"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG81C_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG81C_002541"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG81C_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5065666.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG81C_002542"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG81C_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG81C_002543"
     CDS             complement(2143..2373)
                     /locus_tag="ACG81C_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5065667.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG81C_002544"
     CDS             complement(2603..3421)
                     /locus_tag="ACG81C_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5065668.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG81C_002545"
     CDS             3582..4271
                     /locus_tag="ACG81C_002545"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5065669.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG81C_002546"
     CDS             4285..5787
                     /locus_tag="ACG81C_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5065670.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG81C_002547"
     CDS             5800..6276
                     /locus_tag="ACG81C_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5065671.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG81C_002548"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG81C_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZUMI010000136         6600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000136 DAZUMI010000000
VERSION     DAZUMI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.143"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..467)
                     /locus_tag="ACG81C_002549"
     CDS             complement(9..467)
                     /locus_tag="ACG81C_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5065672.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(480..1811)
                     /locus_tag="ACG81C_002550"
     CDS             complement(480..1811)
                     /locus_tag="ACG81C_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5065673.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1822..3264)
                     /locus_tag="ACG81C_002551"
     CDS             complement(1822..3264)
                     /locus_tag="ACG81C_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5065674.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3251..4015)
                     /locus_tag="ACG81C_002552"
     CDS             complement(3251..4015)
                     /locus_tag="ACG81C_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5065675.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4119..4994)
                     /locus_tag="ACG81C_002553"
     CDS             complement(4119..4994)
                     /locus_tag="ACG81C_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5065676.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5249..5333)
                     /locus_tag="ACG81C_002554"
     tRNA            complement(5249..5333)
                     /locus_tag="ACG81C_002554"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5297..5299),aa:Leu,seq:caa)
     gene            complement(5351..5422)
                     /locus_tag="ACG81C_002555"
     tRNA            complement(5351..5422)
                     /locus_tag="ACG81C_002555"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5388..5390),aa:Gln,seq:ttg)
     gene            complement(5432..5504)
                     /locus_tag="ACG81C_002556"
     tRNA            complement(5432..5504)
                     /locus_tag="ACG81C_002556"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5469..5471),aa:His,seq:gtg)
     gene            complement(5566..5650)
                     /locus_tag="ACG81C_002557"
     tRNA            complement(5566..5650)
                     /locus_tag="ACG81C_002557"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5614..5616),aa:Leu,seq:caa)
     gene            complement(5671..5741)
                     /locus_tag="ACG81C_002558"
     tRNA            complement(5671..5741)
                     /locus_tag="ACG81C_002558"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5707..5709),aa:Cys,seq:gca)
     gene            complement(5787..5858)
                     /locus_tag="ACG81C_002559"
     tRNA            complement(5787..5858)
                     /locus_tag="ACG81C_002559"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5824..5826),aa:Gln,seq:ttg)
     gene            complement(5870..5942)
                     /locus_tag="ACG81C_002560"
     tRNA            complement(5870..5942)
                     /locus_tag="ACG81C_002560"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5907..5909),aa:His,seq:gtg)
     gene            complement(5945..6018)
                     /locus_tag="ACG81C_002561"
     tRNA            complement(5945..6018)
                     /locus_tag="ACG81C_002561"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5984..5986),aa:Trp,seq:cca)
     gene            complement(6025..6105)
                     /locus_tag="ACG81C_002562"
     tRNA            complement(6025..6105)
                     /locus_tag="ACG81C_002562"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6069..6071),aa:Tyr,seq:gta)
     gene            complement(6107..6182)
                     /locus_tag="ACG81C_002563"
     tRNA            complement(6107..6182)
                     /locus_tag="ACG81C_002563"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6147..6149),aa:Phe,seq:gaa)
     gene            complement(6218..6290)
                     /locus_tag="ACG81C_002564"
     tRNA            complement(6218..6290)
                     /locus_tag="ACG81C_002564"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6255..6257),aa:Asp,seq:gtc)
     gene            complement(6300..6372)
                     /locus_tag="ACG81C_002565"
     tRNA            complement(6300..6372)
                     /locus_tag="ACG81C_002565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6337..6339),aa:Val,seq:tac)
     gene            complement(6388..6459)
                     /locus_tag="ACG81C_002566"
     tRNA            complement(6388..6459)
                     /locus_tag="ACG81C_002566"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6424..6426),aa:Glu,seq:ttc)
     gene            complement(6474..6563)
                     /locus_tag="ACG81C_002567"
     tRNA            complement(6474..6563)
                     /locus_tag="ACG81C_002567"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6527..6529),aa:Ser,seq:gga)
BASE COUNT         1978 a         1459 c         1083 g         2080 t
ORIGIN      
        1 atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt ttgttttaat
       61 aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt ttgtgcttgc
      121 tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc ctggttactt
      181 tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc catatggatc
      241 agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg aaatatatta
      301 tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat agcgtatcca
      361 actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg aataatttga
      421 ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt ttatcacctc
      481 tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag tatttttttt
      541 gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc tttttgataa
      601 gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc ctccatcaat
      661 cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc agttgcacca
      721 acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc cccttgatta
      781 ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc tatatcgacg
      841 atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat accgcggcgt
      901 attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat attagcacgt
      961 gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata ctctaagtaa
     1021 ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg aaaggttttt
     1081 tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt tattagtttt
     1141 tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga tgtataccag
     1201 ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata accgtaattc
     1261 actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat ttttacattc
     1321 ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata attcaaaatc
     1381 catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc tttgattgag
     1441 cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa tccatgttta
     1501 agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa
     1561 acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat gcgaatattt
     1621 gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat atttctttct
     1681 tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt gttcaaaatg
     1741 gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat aatctgtttt
     1801 ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg ccataacata
     1861 agtcacttct aagccgatga taatttgata atttttttca gttggtttaa tgatcccgct
     1921 tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta cctctacccg
     1981 taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa acgcttgtag
     2041 catttttgtt gccatatatt cataattttt acttgctaac cctaactctg tttcttctaa
     2101 ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt
     2161 attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc cgattggaag
     2221 aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa tacatgctcc
     2281 gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta aaccggcaaa
     2341 aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt caacaggctc
     2401 agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt tgaccatctc
     2461 tttattttct ttataagctg ctttatagat agctagacat atagctggta taccaaacat
     2521 cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt tgataaagcg
     2581 tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta aatcgtaata
     2641 aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat
     2701 aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat tatcaatccc
     2761 aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt gtagaatcat
     2821 caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg gtaatttttg
     2881 ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc caccaaaaat
     2941 ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag caatcgctaa
     3001 aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga ttgcaactcc
     3061 tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac caattactgg
     3121 gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg gcatcgctgc
     3181 aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat tcatttttat
     3241 tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca tttttcactt
     3301 tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga tacacaaaac
     3361 ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact tcgatcgttt
     3421 tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc gtattgattt
     3481 gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa taatcgacat
     3541 gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat ttttttgcta
     3601 atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga ctgtctaaat
     3661 gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat tcctgataca
     3721 gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg aactctccgc
     3781 tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat gttaaatgga
     3841 ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg aaacccggca
     3901 tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat
     3961 aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt cccatttcat
     4021 tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca tttactatgt
     4081 gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct tgctagatga
     4141 attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg ttcgatgata
     4201 tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa
     4261 ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag gaatttgata
     4321 tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga ataggatttt
     4381 tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt taaactatta
     4441 ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc atttgaccct
     4501 aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat atcatattca
     4561 tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct atcttctctt
     4621 tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt tgaatctaat
     4681 ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc cataaattcg
     4741 attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga atctaataca
     4801 aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga aaagccaatc
     4861 cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc attatgattc
     4921 agctttttca tcacatacac cgttaattca cctcctctat atgataaaac aaaacaactt
     4981 gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa aaacgaccta
     5041 agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca taatttacaa
     5101 aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt ttatgataat
     5161 acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat agcaactctc
     5221 tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc ggcacgtcct
     5281 caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc ggcaaattta
     5341 aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc
     5401 cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga acccacgaat
     5461 gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat aaaagatgtt
     5521 atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg gcggccgggg
     5581 tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct gccaattccg
     5641 ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa ccggtgatga
     5701 aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta ttatcaatct
     5761 ataaaataaa gaataaatta tagaaactgg ggtagctgga ttcgaaccaa cgcatgaggg
     5821 agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaagg gcgaatgatg
     5881 ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc
     5941 gccatggcag gggcagcagg aattgaaccc acactaacgg ttttggagac cgctgttcta
     6001 cctttaaact atgcccctat aaaatggagg agagtggatt cgaaccactg aaccctaagg
     6061 aacggattta cagtccgtcg cgtttagcca cttcgctact cctccatggt ggcgcgggac
     6121 agaatcgaac tgccgacaca cggagcttca atccgttgct ctaccaactg agctaccgcg
     6181 ccatatttta aaattattaa ttgaataaca agaacgacgg ttccgacggg aatcgaaccc
     6241 gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc agttcattat
     6301 ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg
     6361 agctaaacct ccaacattca attaaaatga cccgtacggg attcgaaccc gtgttaccgc
     6421 cgtgaaaggg cggtgtctta accgcttgac caacgggcca ggtaaatttg ctacggagag
     6481 taagggattc gaacccttga gacagtttgg caccatctac atgatttcca atcatgctcc
     6541 ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc tcaaaatgag
//
LOCUS       DAZUMI010000137         6578 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000137 DAZUMI010000000
VERSION     DAZUMI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.144"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1756)
                     /locus_tag="ACG81C_002568"
     CDS             complement(<1..1756)
                     /locus_tag="ACG81C_002568"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065677.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEA"
     gene            complement(1716..3470)
                     /locus_tag="ACG81C_002569"
     CDS             complement(1716..3470)
                     /locus_tag="ACG81C_002569"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065678.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3580..4149)
                     /locus_tag="ACG81C_002570"
     CDS             complement(3580..4149)
                     /locus_tag="ACG81C_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS5065679.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4167..5582)
                     /locus_tag="ACG81C_002571"
     CDS             complement(4167..5582)
                     /locus_tag="ACG81C_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065680.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5593..6261)
                     /locus_tag="ACG81C_002572"
     CDS             complement(5593..6261)
                     /locus_tag="ACG81C_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS5065681.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6392..>6578)
                     /locus_tag="ACG81C_002573"
     CDS             complement(6392..>6578)
                     /locus_tag="ACG81C_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5065682.1"
                     /translation="KTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLL
                     TRRLYQFSVPGWIELLS"
BASE COUNT         2236 a         1207 c          882 g         2253 t
ORIGIN      
        1 gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg agatttttgt
       61 atatttctga ccttcgatac aactcactat gagtaccttg atcaactatt tttccttgat
      121 ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat ataattagta
      181 ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca ttttccgcat
      241 ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa aaagcccgtg
      301 ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct ccgaccaatg
      361 tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa cgagctgctt
      421 ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc aaaatacttg
      481 tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag
      541 atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa tcatacagtc
      601 tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact aacgcagtca
      661 cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg ttatcatatg
      721 agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct gatccctctt
      781 ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa taaaatattt
      841 caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg gctagtaagt
      901 aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca cctactagaa
      961 tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact gaaaaagtac
     1021 ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa agtctgtctt
     1081 gaactttttt agatagatta aagctattaa tctcttgctg caattctata gtttcttgaa
     1141 attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc gtttgggatt
     1201 tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca gctaatccca
     1261 ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga gccataccgc
     1321 cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca gacatgatag
     1381 tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa tttgacaaat
     1441 cgtctgcaat ttgcattctt aaatgcgcac tttctttata agtactatta tataagtttt
     1501 catactccct atctaacaac caatacatga aaagcaaagt aacgaacgat actgtaaaat
     1561 aaagccaagg cggctttaca ttttctaaaa ctaattcgtc tatcagcatc attataatga
     1621 atgctggtac catattaatg cagtatacaa aaaagctaga aatagatgct ttaaccaaat
     1681 cttctgcgcc ctgattagtt aaagcgaatt tgtttttata aaaacttttc attgtcaagc
     1741 ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg atgtttagcc
     1801 attaattcat tatgatttcc acgttctact attttccctt ctgacaagac aagaatttca
     1861 tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt ttctttcatc
     1921 agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga tgcactggct
     1981 tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc tatacgttgt
     2041 ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc tctttcggga
     2101 aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac tttacttgct
     2161 ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa tagtttcgta
     2221 tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg atttaaaggc
     2281 ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat tagtttagct
     2341 atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt ttgttctaaa
     2401 actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac attttcaaat
     2461 gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt
     2521 tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa gtgcatactt
     2581 acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa caacatcaac
     2641 aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt tataggaact
     2701 atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca
     2761 ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt caaggcttta
     2821 aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttcctcggat atattctacc
     2881 gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt tgtattctct
     2941 gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac caatccaatt
     3001 ttaaaattaa tgaaaaagcc taaggcaact gataaaacag gtgttactat ggcttgagca
     3061 ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc atcaattgtc
     3121 tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg
     3181 ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg agaaaataag
     3241 ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg aaccgcataa
     3301 ctcttagcta aatattcatc tcctacaata attagagact taagaaattt gtaaatactg
     3361 taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc taaccatccg
     3421 tagtatttca ttttagggac ataactaaat aattttttgt atactttcat tttttcctcc
     3481 cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg taatagcgta
     3541 aattagagat gtacaaacag atagaaggta aactacgttc tattttttgt atttttttaa
     3601 gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta acactgaaat
     3661 gattccccat atacctaccg catagtggat cattgcatcc attgttgcag cagattgtcc
     3721 tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa tccactgtcc
     3781 ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg ctcgatcata
     3841 tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac tgataaacca
     3901 aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg tgtaaatagt
     3961 aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc cagataataa
     4021 tccaaaaatc cctggggata tgctgtgacc aaacgcccca ccaatactac taaccattat
     4081 tgaaaaaaag taaattacta gggaaataac tgtagctaaa ccaataaaaa tataatcttt
     4141 tgttttcata tatattctcc tcaattctat ttcgttatgt cattaaaaat ctgttctatc
     4201 aaagttgaat cttctactct aagttttata tcttttttta ttccaccatt ttttaaataa
     4261 ataattctat tgcatgtttt ataaagaaat tccggatcat gagtggctat aactactgca
     4321 cccttttctt ttaagtcttt aataaggtca gcaatccttt ccatattaat ataatccaac
     4381 ccacttgtag gttcatcaaa tagaaataaa ttagtttttg acaatgaagc tatagaaacc
     4441 aataatcttt gtttttctcc accggataaa tcaaacggta attgatcttt cattttataa
     4501 agttccattt tttttaaaat tttttcttgc tgatctttac taactatttc attccccagg
     4561 gctaactcgc tcctcactga ttctgtgaaa aattggtaat ccatttcttg cattactaaa
     4621 aacggcgatg acaaattttt tttattttcg attatctttc ctttggtagg gcgaataatc
     4681 ttcatgagta ttcttagtaa ggttgttttc cccacaccgt ttttccctac caaaccaata
     4741 acatctccaa ctttgacatc aaaatttatg tcttttagaa tattttcata agtaatttct
     4801 tcaactttta atacatctgc acttaccttt tcgatatagg gaacttcaat cgaactatta
     4861 aaaatgtcaa atttccttaa tgcgttattg ttttcagttt ttaacaactg aatagttgta
     4921 gtttttacag ttctattttt aacaaataga acttgatcaa ttaattcttt caaatagtaa
     4981 aaacgatgtt cagctataac aatagtaaat cctttatttt ttaaatcaca aagtaagtca
     5041 gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg gttcatccaa tataattaat
     5101 ttagagttta gcgataaact acatccaata gaaattttct gccgttcacc acttgatagc
     5161 gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa ttcattgatt
     5221 cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac taactcactc
     5281 gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc aatatttttt
     5341 gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat ttccccaacc
     5401 aattttccgt cataaagtct tggaataagt ccattcatta actttaatag tgtggatttc
     5461 ccactcccag aagaaccagt aatcaatgta agactacctt tttttattga aaaattaagg
     5521 tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt aaattcaaac
     5581 aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa acaggatgat
     5641 atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag gagattctat
     5701 cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac gtataagaac
     5761 gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc gaatgtttct
     5821 catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa agaatctgat
     5881 cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta aagaggataa
     5941 taattcattg ctagtataga cactaaccaa caaccatgag atcattacga ccggcaacat
     6001 tcgaaataca atatataagg atgtcccaaa aaatatagta aaaaactcta tcttgatagc
     6061 atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat aaatacatgt
     6121 ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc ccacttgaaa
     6181 tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag taatcaaggt
     6241 taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac tgttatctta
     6301 agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa agcataactg
     6361 agaacaattc tcaatgcaag ctttttttta attaactcag taattcaatc cacccaggaa
     6421 cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct
     6481 tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat
     6541 taaagactaa attttgattg atcattccga tagtcttt
//
LOCUS       DAZUMI010000138         6481 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000138 DAZUMI010000000
VERSION     DAZUMI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.145"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..761
                     /locus_tag="ACG81C_002574"
     CDS             24..761
                     /locus_tag="ACG81C_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5065683.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            775..1590
                     /locus_tag="ACG81C_002575"
     CDS             775..1590
                     /locus_tag="ACG81C_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5065684.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1603..2250
                     /locus_tag="ACG81C_002576"
     CDS             1603..2250
                     /locus_tag="ACG81C_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5065685.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2266..2922
                     /locus_tag="ACG81C_002577"
     CDS             2266..2922
                     /locus_tag="ACG81C_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5065686.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3084..3905
                     /gene="racE"
                     /locus_tag="ACG81C_002578"
     CDS             3084..3905
                     /gene="racE"
                     /locus_tag="ACG81C_002578"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5065687.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3908..5263
                     /gene="rph"
                     /locus_tag="ACG81C_002579"
     CDS             3908..5263
                     /gene="rph"
                     /locus_tag="ACG81C_002579"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5065688.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5264..5782
                     /locus_tag="ACG81C_002580"
     CDS             5264..5782
                     /locus_tag="ACG81C_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5065689.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5889..6389
                     /gene="cbpB"
                     /locus_tag="ACG81C_002581"
     CDS             5889..6389
                     /gene="cbpB"
                     /locus_tag="ACG81C_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5065690.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2128 a         1090 c         1436 g         1827 t
ORIGIN      
        1 aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg gataaatatt
       61 atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga gaagttgtcg
      121 ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc aatggattag
      181 agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat aaaaagacaa
      241 aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat ttgtacccaa
      301 ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag caagaccagg
      361 caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta gataagaaag
      421 attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc gccagaggtt
      481 tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg gatccggaaa
      541 tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg tcaatgattg
      601 tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc ttcatggctg
      661 atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat ccaaatgaag
      721 tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa aaagatgcgc
      781 aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc actagccgga
      841 tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc taccaatgaa
      901 atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat tgagagcaga
      961 actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat ccttggtgac
     1021 aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt attgaaaaac
     1081 ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt
     1141 gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa aggaagccaa
     1201 atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa aggttctaca
     1261 tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt agaaaactat
     1321 gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac ggataatgcg
     1381 attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg aacgtttact
     1441 aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa agctgttaac
     1501 caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca aaaatggttt
     1561 ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga attgatacaa
     1621 acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag tgtcttagca
     1681 cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc aaagaataaa
     1741 gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc attattaatc
     1801 atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga tggattccaa
     1861 gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac agtacgttca
     1921 gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg ttttacctat
     1981 tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat cccgccatta
     2041 ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt agctggttta
     2101 gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt cgatacgtat
     2161 atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt aatggtttat
     2221 cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga ttttagcggc
     2281 gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac tgttgaagtt
     2341 tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt gattcgtttt
     2401 gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat acggaatctt
     2461 ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg cattcagatg
     2521 aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat gttgtcggaa
     2581 attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg attatcaacg
     2641 ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg
     2701 atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt agcagtaatc
     2761 atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa caccaattat
     2821 gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta cgcgctttct
     2881 ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat tcttttagcg
     2941 gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa aagagatttc
     3001 gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca caatagggag
     3061 gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct atcggattta
     3121 ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg
     3181 aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga cccgcggaac
     3241 aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga atcaagatgc
     3301 tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa gctgctcttt
     3361 ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa aaaacacaaa
     3421 ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag
     3481 cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc aaatttgttt
     3541 cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg gcagaaacat
     3601 tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc cattatccat
     3661 tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc gattctggag
     3721 cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc aattcaccgc
     3781 aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt ttcgaggaaa
     3841 tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa ttgggaggaa
     3901 aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac
     3961 aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata caacagttat
     4021 ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag gaacaggatg
     4081 ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc gtagagaaag
     4141 cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg gtcgttcctt
     4201 gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag actgcgatgt
     4261 gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg tggcattgaa
     4321 attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa taaaagaaca
     4381 tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag atttggatta
     4441 ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat ctggaaaatt
     4501 cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat
     4561 gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac atgctttgct
     4621 aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca tcattgcgac
     4681 aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg gatatcatgt
     4741 aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca gcacatttga
     4801 agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc cagtcttagc
     4861 agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct attctgcacg
     4921 ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat atgaattgac
     4981 agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat tcgcggcacc
     5041 caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc gtataccatc
     5101 tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga aacaaacagc
     5161 agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa
     5221 attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt atttagtagt
     5281 aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt atcgttctga
     5341 agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc cattatggga
     5401 tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac agaaaaaagt
     5461 gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga atgtccgatt
     5521 tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg tgttttttgg
     5581 tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga atcctggtag
     5641 catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg aaagtttaga
     5701 tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag agttggcctt
     5761 ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa taagctttat
     5821 tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat agaaatggag
     5881 gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag gaaacgtttt
     5941 tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc catggtttac
     6001 ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat gatcactttg
     6061 taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa acaatcagca
     6121 tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt ccgacaattg
     6181 atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct tttttaccgg
     6241 ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg cttaaagccg
     6301 taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat
     6361 taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat
     6421 aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct gggacaaaag
     6481 c
//
LOCUS       DAZUMI010000139         6307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000139 DAZUMI010000000
VERSION     DAZUMI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.146"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACG81C_002582"
     CDS             complement(<1..506)
                     /locus_tag="ACG81C_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5065691.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            817..1809
                     /locus_tag="ACG81C_002583"
     CDS             817..1809
                     /locus_tag="ACG81C_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065692.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1869..3290
                     /locus_tag="ACG81C_002584"
     CDS             1869..3290
                     /locus_tag="ACG81C_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5065693.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            3283..4782
                     /locus_tag="ACG81C_002585"
     CDS             3283..4782
                     /locus_tag="ACG81C_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5065694.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4821..6215
                     /gene="uxaC"
                     /locus_tag="ACG81C_002586"
     CDS             4821..6215
                     /gene="uxaC"
                     /locus_tag="ACG81C_002586"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5065695.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         2042 a         1180 c         1285 g         1800 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      361 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      601 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccctaa ttaaatgagc
      661 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
      721 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
      781 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatatcgct
      841 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
      901 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
      961 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
     1021 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
     1081 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
     1141 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
     1201 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
     1261 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
     1321 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
     1381 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
     1441 ctagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
     1501 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
     1561 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
     1621 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
     1681 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
     1741 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
     1801 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
     1861 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
     1921 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
     1981 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
     2041 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
     2101 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
     2161 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
     2221 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
     2281 cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
     2341 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
     2401 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
     2461 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
     2521 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
     2581 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
     2641 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
     2701 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
     2761 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
     2821 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
     2881 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
     2941 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
     3001 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
     3061 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
     3121 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
     3181 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
     3241 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
     3301 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
     3361 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
     3421 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
     3481 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
     3541 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
     3601 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
     3661 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
     3721 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
     3781 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
     3841 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
     3901 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
     3961 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
     4021 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
     4081 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
     4141 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
     4201 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
     4261 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
     4321 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
     4381 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
     4441 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
     4501 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
     4561 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
     4621 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
     4681 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
     4741 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
     4801 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
     4861 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
     4921 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
     4981 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
     5041 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
     5101 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
     5161 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
     5221 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
     5281 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
     5341 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
     5401 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
     5461 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
     5521 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
     5581 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcttacag
     5641 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
     5701 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
     5761 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
     5821 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
     5881 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
     5941 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
     6001 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
     6061 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
     6121 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
     6181 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
     6241 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
     6301 ccaaatt
//
LOCUS       DAZUMI010000140         6303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000140 DAZUMI010000000
VERSION     DAZUMI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.147"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG81C_002587"
     CDS             334..714
                     /locus_tag="ACG81C_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5065696.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACG81C_002588"
     CDS             696..1610
                     /locus_tag="ACG81C_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5065697.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACG81C_002589"
     CDS             1855..2544
                     /locus_tag="ACG81C_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065698.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACG81C_002590"
     CDS             complement(2665..2751)
                     /locus_tag="ACG81C_002590"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5065699.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACG81C_002591"
     CDS             3427..4377
                     /locus_tag="ACG81C_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5065700.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACG81C_002592"
     CDS             4398..4931
                     /locus_tag="ACG81C_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5065701.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACG81C_002593"
     CDS             4947..5117
                     /locus_tag="ACG81C_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065702.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACG81C_002594"
     CDS             5567..6100
                     /locus_tag="ACG81C_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065703.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACG81C_002595"
     CDS             6112..>6303
                     /locus_tag="ACG81C_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065704.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2324 a         1103 c         1173 g         1703 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtacacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZUMI010000141         6267 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000141 DAZUMI010000000
VERSION     DAZUMI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.148"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG81C_002596"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG81C_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5065705.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACG81C_002597"
     CDS             499..861
                     /locus_tag="ACG81C_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5065706.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACG81C_002598"
     CDS             913..2595
                     /locus_tag="ACG81C_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5065707.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACG81C_002599"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACG81C_002599"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5065708.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACG81C_002600"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACG81C_002600"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5065709.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACG81C_002601"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACG81C_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5065710.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2080 a         1092 c         1430 g         1665 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcga
//
LOCUS       DAZUMI010000142         6238 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000142 DAZUMI010000000
VERSION     DAZUMI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.149"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1012
                     /locus_tag="ACG81C_002602"
     CDS             5..1012
                     /locus_tag="ACG81C_002602"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS5065711.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1024..2028
                     /gene="neuB"
                     /locus_tag="ACG81C_002603"
     CDS             1024..2028
                     /gene="neuB"
                     /locus_tag="ACG81C_002603"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS5065712.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2025..3173
                     /gene="neuC"
                     /locus_tag="ACG81C_002604"
     CDS             2025..3173
                     /gene="neuC"
                     /locus_tag="ACG81C_002604"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS5065713.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3146..3823
                     /locus_tag="ACG81C_002605"
     CDS             3146..3823
                     /locus_tag="ACG81C_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS5065714.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3774..4955
                     /locus_tag="ACG81C_002606"
     CDS             3774..4955
                     /locus_tag="ACG81C_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS5065715.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            4968..5945
                     /locus_tag="ACG81C_002607"
     CDS             4968..5945
                     /locus_tag="ACG81C_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5065716.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5945..>6238
                     /locus_tag="ACG81C_002608"
     CDS             5945..>6238
                     /locus_tag="ACG81C_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5065717.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESID"
BASE COUNT         2327 a          787 c         1198 g         1926 t
ORIGIN      
        1 ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga
       61 aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga
      121 agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat
      181 tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt
      241 tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa
      301 aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca
      361 ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa
      421 gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat
      481 attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt
      541 tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt
      601 tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt
      661 tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt
      721 aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg
      781 tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc
      841 atatgaaaat gataaaatgt atatcattcc accacactat catgctactg acgaactgta
      901 caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag
      961 tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt
     1021 atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta
     1081 gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt
     1141 caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag
     1201 gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa
     1261 tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact
     1321 ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt
     1381 ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca
     1441 gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc
     1501 atagatagtc cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat
     1561 attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc
     1621 gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt
     1681 gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat
     1741 cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat
     1801 ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta
     1861 gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa
     1921 cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa
     1981 agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta
     2041 tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac
     2101 aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat
     2161 ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt
     2221 ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata aatggtttca
     2281 caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa
     2341 tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag
     2401 gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg
     2461 ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt
     2521 ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta
     2581 gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc
     2641 aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga
     2701 tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg
     2761 gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa aacggatatt atttatatta
     2821 cgaatttaga gccggaggaa tacctgacta tattaaaaga gtgcaaattt atgatcggaa
     2881 attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata aatttaggaa
     2941 ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta aatgaaaatg
     3001 aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg
     3061 ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa
     3121 tattgaatga tggggaagat attaagtgga ttcaaaaaca aatagcttat taaaatatac
     3181 tagtgatcca tacattatta ttggggctgg cggacacgca aaagtcatta ttgaaacctt
     3241 aagattagaa aataagttaa tatatggatt ttttgatgat aagcaaaata aaatgcaaga
     3301 tctgaaatat cttgggaaaa taaacgaatt gaatagctgg gcggatagca aaaaaaaaat
     3361 ctataaattt attgtagcta ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt
     3421 gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag tggcaaaaga
     3481 aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc cagatgctgt
     3541 aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt gtagaattaa
     3601 taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg taggtgtagg
     3661 aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat cttggtgtat
     3721 gataggcgca gggagcacta ttgttaagga tattccctca cattccttag cttatggcaa
     3781 tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta ttatcaagtc
     3841 ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat aattggatag
     3901 cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat actggttcta
     3961 agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc ttaagactgc
     4021 ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata gctacagtaa
     4081 atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct aatacatgga
     4141 atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac aaccaattac
     4201 ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa cgaattttag
     4261 ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg ttaggttcta
     4321 cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca tttaacggta
     4381 ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa aaatatgttg
     4441 agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat caacatagcg
     4501 aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt ggacaaatgg
     4561 aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat aaggaagctt
     4621 tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt
     4681 ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt aacgtaataa
     4741 ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta catttgcaac
     4801 cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg tcagaaaagt
     4861 tatttaatag cggcttatgc ttaccttctg gaagtaatat gacagatagt gaacaagaat
     4921 ttgttattga tgagtttaaa aaaatattca agtaggtgtg atattttatg aacataggaa
     4981 ttattggttt aggttccatg gggaaaagaa gacttagact cattcaagaa cactttaatg
     5041 atattttact atgtggtgtt gatactaatg aggcgagacg aaaggaagtt gaaaaggaat
     5101 ttggaatacc aacttataat agtctaacag aggcaaaaaa tttgtttgaa ataaattcag
     5161 tttttgtttg tacatcccct ataacccatg aatttattat aaaagaggct ttaaatttag
     5221 attgtaatat ctttacggaa atcaatttat taaatgaata ttatgatgaa ataatcgaag
     5281 tagctaaaaa gaaagataga ttactttatc tatcttctac atttttgaag agaaaagaaa
     5341 ttcaatatat taagtctaaa gtgacgcata ataaaaaaat ttcttatcaa taccatgtag
     5401 gacagtattt acccgactgg catccttggg aagattatcg caattttttt gtttcaaaca
     5461 aaaaaactaa tgggtgtaga gaaatattcg ctattgaatt accttggatt atagatacat
     5521 ttgggaaagt taaatcgttt actgtaatga cccagaaatt atccaatctt gatattgatt
     5581 attcggacag tttcactctt attattgagc atgaaactgg tatagttgga actttaagtg
     5641 tagatgttgt ttcaagagta gctaaaagaa atttaactat aattggtgaa aagattttaa
     5701 tagaatggga aggtaacccg gattctctat tttcatggaa tgaagaaact tctagtatga
     5761 cagatattca attgtacgat gaggttatgc atgataataa ttattcacat actattattg
     5821 aagatgcata tttagaggag attaaagaat ttttcacgct tttggaaaag aaaatggaac
     5881 cccaatatac ttttgaaaaa gataagtata ttattaactt aattaacgac attgaggtaa
     5941 attaatgatg aaagtaattt tttttgggtt agggtcaatt ggaaagagac atctaaaaaa
     6001 tctaataagc tatggaaaaa agaattctat taattttgat ataacagctt atagtagcag
     6061 tggaaaaaaa gagtttgaag aaataacgta tattcaaaat agagacgatt tggatacaca
     6121 ttatgatata gcttttatta caaatccgac ttttaagcat ctggaaacac taaatgtaat
     6181 tgttgaaaaa gttgattaca tatttttaga aaaacctgct ttttctgaga gtattgat
//
LOCUS       DAZUMI010000143         6230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000143 DAZUMI010000000
VERSION     DAZUMI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.150"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACG81C_002609"
     CDS             133..633
                     /locus_tag="ACG81C_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5065718.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACG81C_002610"
     CDS             730..2577
                     /locus_tag="ACG81C_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5065719.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACG81C_002611"
     CDS             2580..3476
                     /locus_tag="ACG81C_002611"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5065720.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACG81C_002612"
     CDS             3526..3915
                     /locus_tag="ACG81C_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5065721.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACG81C_002613"
     CDS             3902..>6230
                     /locus_tag="ACG81C_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5065722.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZUMI010000144         6218 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000144 DAZUMI010000000
VERSION     DAZUMI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.151"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1181)
                     /locus_tag="ACG81C_002614"
     CDS             complement(51..1181)
                     /locus_tag="ACG81C_002614"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5065723.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1241..1468)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1830..3032)
                     /locus_tag="ACG81C_002615"
     CDS             complement(1830..3032)
                     /locus_tag="ACG81C_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5065724.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3092..3295)
                     /locus_tag="ACG81C_002616"
     CDS             complement(3092..3295)
                     /locus_tag="ACG81C_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5065725.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3437..4504)
                     /locus_tag="ACG81C_002617"
     CDS             complement(3437..4504)
                     /locus_tag="ACG81C_002617"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5065726.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4562..4669)
                     /locus_tag="ACG81C_002618"
     CDS             complement(4562..4669)
                     /locus_tag="ACG81C_002618"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065727.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4832..5644
                     /locus_tag="ACG81C_002619"
     CDS             4832..5644
                     /locus_tag="ACG81C_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065728.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6018..6209)
                     /locus_tag="ACG81C_002620"
     CDS             complement(6018..6209)
                     /locus_tag="ACG81C_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065729.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1834 a         1281 c          990 g         2113 t
ORIGIN      
        1 catgaaaagc cgactttttg ggacgctttt ttttgccaag cctcacattt ttatagccaa
       61 acttcttttg cgacttgctg caataattgg attttcttcc attgttcttc ttctgttaaa
      121 tggtttcctt cttctgttga agcaaaccca cattgaggac tgatacttag attttccaat
      181 ggtacaaatt gttgtgcttc ttttattcgt tcttttattt tctccgtatc ttccaaatct
      241 ggaaacttag aagtaactaa tcctaaaaca actttacttt ctttgttact ccaatgtctt
      301 aaaggagtaa aatctcctgc tcggtcagaa tcgtattcca aaaagaaacc atcataattt
      361 gttgaaaaca actcttctgc aatgggttca tatccaccgg aaaacagcca agaagaagcg
      421 tgattcccgc ggcagatatg tgttgtgatc gtcaaatctt ctggtttgtc ttccagtgcg
      481 ttattgataa tctctgtaca aatatgggcc atttctttcg gatcaattcc tttttgaaga
      541 agtgcttctc ttttagtttg atcagctaga aacgcccaat ttgtatcatc gaattgcagg
      601 taacggcaac ctttttcata aaaagcatga atcgctgctt tgtaagtaga ggacaagtcc
      661 tcataaaaat cagatagatc aggataaatc tccttgattt ttgaagaacc atcattagat
      721 aggatctctt gtcgtaaaat catcgtggga ctagggatcg ttgcttttgc aacaaaagta
      781 tcatctgttt ccactacttc tttcagaaaa gcaaaatctt ttaaaaatgg gtgatttgga
      841 ttgaaagata atttatctac tacccgaata ttataagctg gtgctgcttt tccgtggaat
      901 gtttgattat atccagtttc tggaacatag ccttcgatac catttagttc ttctaaaaaa
      961 tcaagatgcc aaaatccacg ccgaaattca ccatctgtaa ttcctttgat gccgactgct
     1021 ttttggtctt ccactaattt ttttatttcc tggtcttcga ttgcacgaag ttcttctttt
     1081 gaaagtaagc cttcttgaac tttttttctt gcttctttga tcgattgcgg gcgtaagaaa
     1141 cttccgacat gatctactgt aaatggtcta tttttgctca tttcactcac tcttttcttt
     1201 gttttttgta aaaaaaaatc cttttactag caaatactag taaaaggacg attatactcg
     1261 tggtaccacc ttttttcaga aaaaacattc tttctctcag cctgacaata aaatcagtgg
     1321 tcttggtaac gaaagactgt tcgtttttgc ctacctttcg acaaaaaatc ctctaaggcc
     1381 attttcgaca gtcaacttct agctttttac accaaccaaa gcctctcttg aaccattttc
     1441 tgtctacttt ccttctcatc ggatactatt agaataaaat tagaatagca tgtttgaaag
     1501 aaaagtcaat agggaataag aacaaacaaa aactaatttg ttattcttag taatttgttt
     1561 gtggaataca ataaagcttt tctaatatga taattgttca gtcgtccttt catcttagat
     1621 cacttcctta aaatgatcat acccgtttca accacataaa aaaggactta attttgaaaa
     1681 atttcccatc aaaactaagt cctttttatt atagttatgc aaatttgctt attttctcgc
     1741 tatttccgat acatcttgaa caggagcaat tcgactgaaa acactggtgc aaaagcattt
     1801 atttgtctta tggtagaacc gtggaaaact cagactggta ataaattgtc attttttgaa
     1861 agaatagcgc cctcttgaac attatcaatc gcacttacca cttgttgatc tgactttgca
     1921 tataagtgac cgtaagttcc taaagtgatt tgaatattcg catggcccaa tcgttcttga
     1981 atagatagcg gttgaatatt ctgctcaatt agaaatgcaa catgagaatg tcgtagcgcg
     2041 tgaagcttaa tcggtttgac acccgctttt ttagcagctt cttttaacca gttcgcaaaa
     2101 gaataaggtg gcgtaaatgt tccgtccaac tgagcaacat attcaaaatt tccaatacgt
     2161 ttttgcaagt cataccactg tctgagtttt aaggaaagct tcttccccaa cccaatcgtt
     2221 cgataagcac atttagtttt gggagtcgaa aagtaccaat catcttttgt attataaatc
     2281 aatgtcttct caatcgttat cgtgtttttc tcaaaatcta cattcgacca agacaaagct
     2341 tcaagttctc caatccgaat gccagtataa aagagcattt catacacaat cttccgatgg
     2401 tgggtttgaa tcttatcatc cttcaattca ttcatcactt tttgaaactc actgtgttcc
     2461 caaaactcaa ctttcttatt ctcagaagga agcgaagaaa catcttcaac gggattttta
     2521 gcaatcaccc cctctttaac agcccgatcc aatacaattt ttaggtgccc tcgaatccga
     2581 ttgatataag attttgataa cgtttcgcca ttaacttttt tccgtgaatt acgtgactct
     2641 gtctggctta gttcaaataa ccaatcctca atatcgtgac ttgtaatatc tcgaacacga
     2701 aaatgataga aaaactgcat acgcttttcg aaaatgaatt gcgcattctc atatgtttga
     2761 cttttgacct gtctctcgta ccattttaca aacctatcct tgtaaaactc ttcgaaagtc
     2821 atttgattgg tagaacacag ttgattagga attgtcttgt taaaatcatg tgtttccatg
     2881 tacttgtcat aagcaatttt ggcatctttt tgtgaagcat aacctcgttt agtcttctga
     2941 acctttcttc cattttcatc tatacctaga tacgctcgga agaaccattt ttttgtcttt
     3001 ttatcttggt aaatattttt aatttttggc atcgttccat tcctccctgt acttgagaaa
     3061 agctacttta atagtaccgc gcttgtactg tttattccgc aacttgaatg tgaaaaagat
     3121 gttcaattac tcgggatggc accacaccaa cttggcgatt attataaaaa tcaatacctt
     3181 caatccttgc aagatatgcc ttgctctctt taatcattcg tgcagcttga ctaggtttaa
     3241 agcccatctc tatcaaaatt gcacgattta ccatgaaatc acattttgtt tccattctct
     3301 cacctcctga ttctttactc cttttaaaat aatcctcttt ataatctatt gtttctcatt
     3361 cgatggttgt gttatactcg tcttgtcgag gcggagtacg ttgcacaact tgttgtgtaa
     3421 gcagtacttt gcttttttag tagcttgttt tctcatttga tatactttgt aattttcgtt
     3481 cataatactc aacctcctcc tgataatact ccgggtctgc cttcatattt tggagaagtt
     3541 tgatctgtct tggattcatt tctccttgtt caataatcaa aggcaacaag gaagaatgaa
     3601 acgtttgatc gacctgtgca ataaaaacta atgatggtga aacaccatga attaaccaac
     3661 tcatagtttt ctcaaaactt tgaggaattg tatctaaaga aatggtgatt gcgccataat
     3721 tgctaataaa ggctgcccat ttggcatcaa cttttgcacc tagatcattt ggatcacggt
     3781 cataaaaaca aattgctcca ttaagtaact caaagaacag tttggttaaa tcatgggttt
     3841 ttgaaataat ctttgccaag ctttgcgctt tctcttttcg ataccgtagt tcaaagcgat
     3901 tctttacttt tgcctcttct aaaggaattc ctcgtcgctt ccgttgttca tggtccttct
     3961 gataaaagca aaaacgacac tgactcttct ttgagcctaa atacaaggtc tcaccatttg
     4021 attcattgtt tatgatatcc gtaccgccat gagattcact cacttgaaaa gtggtccaca
     4081 catggcctag tattacctta tgcttaagtt ctggaatgct gagactcccc acaaaatcat
     4141 ctaaagtaaa atcaatccgt gtaaagttcc cctgataatc taatacctta cgaaaaaact
     4201 gttgccaatt tgtttttcgt gtttttagcc atcttgctaa gtgtttacag ccttgacctg
     4261 aaaactccat cacagttcct ttaaccgcat cgtcaatgga ataccgaatc gtgattactt
     4321 gattccacac gtattggcca atatagccct ttggcgcata atccagttcc tcaaaacgtt
     4381 caatgggcat ttcaataatc tcttcaatta gctttaaatg ttgattcgag aaaaagtgaa
     4441 tggttagata atcaatcaaa acacggttaa ctggaacgac ctcttcttgc aaactatcca
     4501 acacaagatt caatttttct tcacttaatt gtctcgttcc tttttctact ttcgataata
     4561 actatctcga aatccccact ttttctgcta aatatcgttg tgtccacccc ctttcttctc
     4621 gagaaactcg aatggcttca ccgagatcat gttgattaac ttggttcatc gacaaacctc
     4681 cttctgctat ttacttgtca aagaacctat tgaacttatt ttaagtacat ttttattgta
     4741 cgcctgtatt tattttaagt caatatgttt tgactttttt taagtacaca cgtatactaa
     4801 atttaagaac ttatcctgag gaggagtttt catggagata gacaagcaag cagtcggcaa
     4861 tcgaattcgg cagattcgcc aagaattaaa gttgagtatg gagaaatttg gcaaactaat
     4921 tggtgattta ccgcgaagta cagtcaataa ctgggaacgt gggatcaacc ttccaaaaac
     4981 cgaaaccctt caccaaatcg cagagatagg tcatgtcacc aatgaatatc ttttgtatgg
     5041 tgatcaagag aatcaataca ttttagaaat gctccaaaaa aaggcaggta agcttgatcc
     5101 taagattgaa ggtctcatag tagacgaaat caaacacgct gaccttgtta gtgaaaaaga
     5161 aatggatcga atgattgaat tttttattac aaatctcatt ccaccaactg aacaagacca
     5221 gtttactttc caatgtatcg atgaaaaaaa gcagctttat cttggttcaa ctaattttgg
     5281 taaggaagcc cagctctatt tgcaacatga taatcaaaat catattctgc acatgatgcc
     5341 ctttaccttc tccaacttta gtgtggatcg catactcgtc tatttagcaa atcaaaaatc
     5401 ctattcctat tttgggaaac atttacccaa ggaactaggt gagaaagcca tccttctata
     5461 ctcaattaat caattgacca gcgatgtgcg aattgccccg ttggtctatt cagaagaaac
     5521 cgcttcttat cagtacacag aggacaatca atatctacta gaacagcaat atctttatct
     5581 accattcgtg atggaagtcg agaaagaacg cctgttaaat gcagcgtatc catcatcaaa
     5641 atgaggtgac aaaattgata agcacgtgac aaagaaaata ctgctaggac actccccaat
     5701 gggtagttgt cacagtttcc tgttctttgt tagccccctg ttagaaccgg gggctttatt
     5761 cctgcagtcc ggctacgcct tcctgcaata gacaaaactc cagatcgggt aattcgcttg
     5821 tggcgaaacc cgacctggag tttgtctctg tattttgaac cctaaaaatt aatcaaacag
     5881 actgatattc tcataatcag cgcgaaataa gccattaagt acagttttcc tacctatttt
     5941 tgataacccg ctggttagtt aatattcctt ttccgagaga gataaatgat tggttaaatc
     6001 tatatacatc tcaaccttta aaaatgtttt cgaaatactt catcagtaca tgtcatacaa
     6061 attaccagtg cattctcaaa ttctagttct ttaattgatt tcagttgagg agcagttact
     6121 tttggcggaa atttccattt ttgtccaaac ctcttccccc gatgaaattt ctttgttgca
     6181 attattgcaa tgaaaaattt ttgtttcaaa aatataca
//
LOCUS       DAZUMI010000145         6181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000145 DAZUMI010000000
VERSION     DAZUMI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.152"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG81C_002621"
     CDS             127..579
                     /locus_tag="ACG81C_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065730.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG81C_002622"
     CDS             593..1282
                     /locus_tag="ACG81C_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065731.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG81C_002623"
     CDS             1407..3758
                     /locus_tag="ACG81C_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5065732.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG81C_002624"
     CDS             3838..4233
                     /locus_tag="ACG81C_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065733.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG81C_002625"
     CDS             4558..5175
                     /locus_tag="ACG81C_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065734.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG81C_002626"
     CDS             5216..5515
                     /locus_tag="ACG81C_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS5065735.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG81C_002627"
     CDS             5549..5716
                     /locus_tag="ACG81C_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5065736.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..>6181)
                     /locus_tag="ACG81C_002628"
     CDS             complement(5761..>6181)
                     /locus_tag="ACG81C_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065737.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2129 a          850 c         1232 g         1970 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 t
//
LOCUS       DAZUMI010000146         6174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000146 DAZUMI010000000
VERSION     DAZUMI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.153"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG81C_002629"
     CDS             725..811
                     /locus_tag="ACG81C_002629"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5065738.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACG81C_002630"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACG81C_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1867..2781)
                     /locus_tag="ACG81C_002631"
     CDS             complement(1867..2781)
                     /locus_tag="ACG81C_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5065739.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACG81C_002632"
     CDS             complement(2763..3143)
                     /locus_tag="ACG81C_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5065740.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACG81C_002633"
     CDS             complement(3593..3880)
                     /locus_tag="ACG81C_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5065741.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACG81C_002634"
     CDS             complement(3900..4124)
                     /locus_tag="ACG81C_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5065742.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACG81C_002635"
     CDS             complement(4579..4749)
                     /locus_tag="ACG81C_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065743.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACG81C_002636"
     CDS             complement(4764..5297)
                     /locus_tag="ACG81C_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5065744.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACG81C_002637"
     CDS             complement(5318..>6174)
                     /locus_tag="ACG81C_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5065745.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAZUMI010000147         6068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000147 DAZUMI010000000
VERSION     DAZUMI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.154"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG81C_002638"
     CDS             832..1566
                     /locus_tag="ACG81C_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5065746.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG81C_002639"
     CDS             1582..2751
                     /locus_tag="ACG81C_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5065747.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG81C_002640"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG81C_002640"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5065748.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG81C_002641"
     CDS             3841..4767
                     /locus_tag="ACG81C_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5065749.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG81C_002642"
     CDS             4786..6036
                     /locus_tag="ACG81C_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5065750.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1247 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZUMI010000148         5954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000148 DAZUMI010000000
VERSION     DAZUMI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.155"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACG81C_002643"
     CDS             <1..813
                     /locus_tag="ACG81C_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS5065751.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACG81C_002644"
     CDS             786..1229
                     /locus_tag="ACG81C_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065752.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACG81C_002645"
     CDS             2399..3367
                     /locus_tag="ACG81C_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5065753.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACG81C_002646"
     CDS             3384..3908
                     /locus_tag="ACG81C_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5065754.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACG81C_002647"
     CDS             4284..4565
                     /locus_tag="ACG81C_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065755.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACG81C_002648"
     CDS             4567..4983
                     /locus_tag="ACG81C_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065756.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACG81C_002649"
     CDS             5613..>5954
                     /locus_tag="ACG81C_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFS5065757.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAZUMI010000149         5945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000149 DAZUMI010000000
VERSION     DAZUMI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.156"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1128)
                     /locus_tag="ACG81C_002650"
     CDS             complement(34..1128)
                     /locus_tag="ACG81C_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5065758.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1317..2078
                     /locus_tag="ACG81C_002651"
     CDS             1317..2078
                     /locus_tag="ACG81C_002651"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5065759.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACG81C_002652"
     CDS             complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACG81C_002652"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5065760.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2853..3608
                     /gene="fabI"
                     /locus_tag="ACG81C_002653"
     CDS             2853..3608
                     /gene="fabI"
                     /locus_tag="ACG81C_002653"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5065761.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3718..4770)
                     /locus_tag="ACG81C_002654"
     CDS             complement(3718..4770)
                     /locus_tag="ACG81C_002654"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5065762.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4896..5816
                     /locus_tag="ACG81C_002655"
     CDS             4896..5816
                     /locus_tag="ACG81C_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5065763.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1805 a         1191 c         1072 g         1877 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1201 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1261 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1321 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1381 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1441 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1501 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1561 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1621 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1681 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1741 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1801 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     1861 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     1921 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     1981 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2041 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2101 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2161 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2221 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2281 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2341 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2401 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2461 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2521 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2581 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2641 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagtat catattccta
     2701 aatatttagc aatctttttt taatttaaga aaaaatagtt tgacaatcaa aacattttga
     2761 ctaataatta agaaatcaag aagttatttt agctattatt ctctattttt ttacgactat
     2821 caaattttta tcagaaacaa aggagagtca ttatgtcatt tctagaagga aaaaaaatcg
     2881 tcattatggg cgtcgctaac aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga
     2941 aacaaggggc agaaattatc tatacttacc aaaatgaacg aatgaaaaaa tcattagtca
     3001 aattggtcgg cgaagaagca ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg
     3061 aaaaagcaat gaatgccatc ggtgaatatg ctggggagat ccacggcctt gtccatgcag
     3121 ttgcttacgc caacaaagaa gaattgtccg gaaatgtgtc tgatatttcc agagacggct
     3181 atcaattagc tcaagatatc agcagctact ctttaattgc tgtatcaaaa tacgcacaac
     3241 cgtttcttgc accaaacagc gggatcgtta gcatgactta tctaggttca gaacgcgcaa
     3301 tcccgaatta taacatgatg ggtattgcga aagcttctct tgaagctgct atccgttatt
     3361 tggcagctga gttctcgcct aaaggaattc gggtcaatgg aatctcagct ggggcaatca
     3421 aaacattagc tgtcacaggt gttaaagact atcaaaaact gatccagtta tctgaatctc
     3481 gtacgcctga tcagcaaggt gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa
     3541 tcagcccact ttccagcgga atcatcggtg atattatcta cgttgacaaa ggtgtccatc
     3601 tttcataaac ttttgttgca ttctttaaag taaaacatat aaaaatcagg aaaaaacttt
     3661 ttaaagtctc ttcctgattt tttcttttta cgtttttttc aactcattac tttgaaatta
     3721 ttccacgact tcaatccagc cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa
     3781 ttcatcatac aaccgttttg ttactggtcc tacttccgtt tcactataaa atacatgaag
     3841 attgttattg tgataaatcc caccgattgg agaaataaca gccgctgtac cacatgcacc
     3901 agcttctgca aattgatcta gatcatcaat gaaaatatct ccttcaattg cttgcagtcc
     3961 tagtcgttct tcagctaact gcaacaagga atatttcgtg atacttggta agatagaggg
     4021 cgattgcgga gtgatgaatt ttccgtcttt tgtaattcca aagaaatttg ctgcacctac
     4081 ttcttcgatt ttcgtatgtg tggctggatc aagataaaca caatctgagt aatccagttc
     4141 ttttgcttct gttcctggaa gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc
     4201 agtcccctta ggagcagcac gatcgtaatc agatacaata aaattcgttg gttttagtcc
     4261 acctttgaaa tacgctccta ctggcgtaca gaagacgaca aataaatatt ctggagctgg
     4321 atggacacca atgttttcac cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc
     4381 tgttccatag ggcggcacaa aatcttcatt tgcttttacc acttgcttga cagcatcaac
     4441 gaacatcttt tcagggacca ttggcatcag caatcgtcta gcactttgat tcatccgtcg
     4501 actattttgt tcaggtcgaa agagattgac cttgccgtct tttcgacgat aagcttttag
     4561 accttcaaaa cattgctgac cataatgaag cgctggagag ccttcatgaa tcgtcaggta
     4621 attatcttct gtcaattctc cttcttccca ttgtccgtct ttccatttgg caataaaacg
     4681 ccaaggagtt ttgatataag aaaaacctag attgttccaa tcgatttgca tatacttacc
     4741 accttcgtta ttttctccac attttatcat tatccatatt atttgtcatt ttattttcag
     4801 cttttttcct tcattatagt tatttttgct atttcctttt ttgaaaactt cttggtacaa
     4861 tacagaaaga atcaaatttt agaaaagagg attttatgtt ttcattatta gcaactgccg
     4921 gtacgacaga ctcttctggc gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt
     4981 ttcagcggtt ttgggacggg attgattggg acgccatttt ggctgccttt atccaaaaaa
     5041 gtttatatct gatttttttg atcgtccttt tttggattct tcaccgtgtg ggaaaatatc
     5101 tgattgataa gtcttttaat cagtattcta aaaaagccat tctaaacgag agccgcttaa
     5161 agactttaca tagtctttta aatactattt ttcattatac ccttggtttc ttttttatct
     5221 atgcaatttt atctgctatc ggtgtgccga tcggatcact tttggctggt gccggaattg
     5281 caggtgtggc aatcggtctt ggtgcccaag ggttcatgag tgatgttatt accggatttt
     5341 ttattatcat ggaacaacag atggatgttg gggactacat caagctagca aacttaacga
     5401 ttgaaggaac cgtcgtatct gttggtatcc gtacccttca gctgaaagca acggatggta
     5461 ctgtccattt catcccgaat cgaaacatca ccacgatcag caatctctct cgtgcaaata
     5521 tgcaagtact tttagatatt cggattgttc ctgaagaagg atatgacaaa atctatgaga
     5581 tcattgatcg agtaaatcgt acattagctg aaaaatacca agaagagctc caaactgaac
     5641 cttctatctt tggattagtg gatcttggaa acagcaattt tgcgattcgt acggtctgct
     5701 acgcattgaa cggaaaacaa tatgccttaa aagaagagtt cttgagctcc tatgtcaaag
     5761 aattgacagc ttctggtttc acgattccga acagtccaat tgctgtcgga aaataatttg
     5821 gacaaacgat gcaacgaatc tcactaaaaa gagcgtggga caaaagtaaa gaacactttt
     5881 gtcccacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc
     5941 tgaaa
//
LOCUS       DAZUMI010000150         5857 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000150 DAZUMI010000000
VERSION     DAZUMI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.157"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..796)
                     /locus_tag="ACG81C_002656"
     CDS             complement(<1..796)
                     /locus_tag="ACG81C_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5065764.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPY"
     gene            complement(846..2342)
                     /locus_tag="ACG81C_002657"
     CDS             complement(846..2342)
                     /locus_tag="ACG81C_002657"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5065765.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2536..4158)
                     /locus_tag="ACG81C_002658"
     CDS             complement(2536..4158)
                     /locus_tag="ACG81C_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5065766.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4486..5799)
                     /locus_tag="ACG81C_002659"
     CDS             complement(4486..5799)
                     /locus_tag="ACG81C_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5065767.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         1781 a         1254 c          952 g         1870 t
ORIGIN      
        1 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
       61 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
      121 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
      181 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
      241 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
      301 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
      361 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
      421 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
      481 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
      541 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
      601 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
      661 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
      721 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
      781 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
      841 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
      901 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
      961 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
     1021 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
     1081 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
     1141 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
     1201 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
     1261 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
     1321 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
     1381 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
     1441 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
     1501 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
     1561 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
     1621 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
     1681 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
     1741 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
     1801 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
     1861 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
     1921 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
     1981 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
     2041 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
     2101 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
     2161 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
     2221 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
     2281 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
     2341 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
     2401 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
     2461 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
     2521 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
     2581 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
     2641 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
     2701 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
     2761 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
     2821 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
     2881 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
     2941 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
     3001 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
     3061 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
     3121 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
     3181 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
     3241 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
     3301 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
     3361 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
     3421 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
     3481 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
     3541 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
     3601 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
     3661 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
     3721 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
     3781 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
     3841 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
     3901 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
     3961 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
     4021 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
     4081 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
     4141 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
     4201 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
     4261 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
     4321 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
     4381 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
     4441 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
     4501 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
     4561 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
     4621 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
     4681 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
     4741 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
     4801 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
     4861 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
     4921 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
     4981 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
     5041 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
     5101 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
     5161 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
     5221 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
     5281 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
     5341 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
     5401 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
     5461 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
     5521 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
     5581 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
     5641 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
     5701 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
     5761 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
     5821 acattataaa taaagtccgt taaaatttcg atttatt
//
LOCUS       DAZUMI010000151         5808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000151 DAZUMI010000000
VERSION     DAZUMI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.158"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG81C_002660"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG81C_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5065768.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACG81C_002661"
     CDS             complement(2240..4426)
                     /locus_tag="ACG81C_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5065769.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG81C_002662"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG81C_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5065770.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACG81C_002663"
     CDS             complement(4648..5088)
                     /locus_tag="ACG81C_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5065771.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACG81C_002664"
     CDS             complement(5162..5701)
                     /locus_tag="ACG81C_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5065772.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1503 a         1305 c         1015 g         1985 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DAZUMI010000152         5759 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000152 DAZUMI010000000
VERSION     DAZUMI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.159"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG81C_002665"
     CDS             complement(<1..300)
                     /locus_tag="ACG81C_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5065773.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG81C_002666"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG81C_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG81C_002667"
     CDS             complement(1014..1304)
                     /locus_tag="ACG81C_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065774.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG81C_002668"
     CDS             1706..1942
                     /locus_tag="ACG81C_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065775.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG81C_002669"
     CDS             1949..3901
                     /locus_tag="ACG81C_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5065776.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG81C_002670"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG81C_002670"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5065777.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG81C_002671"
     CDS             complement(4797..5489)
                     /locus_tag="ACG81C_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5065778.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1887 a          994 c          916 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatt
//
LOCUS       DAZUMI010000153         5655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000153 DAZUMI010000000
VERSION     DAZUMI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.160"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..443
                     /locus_tag="ACG81C_002672"
     CDS             <1..443
                     /locus_tag="ACG81C_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFS5065779.1"
                     /translation="YELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANI
                     FNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLS
                     MFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            724..1944
                     /locus_tag="ACG81C_002673"
     CDS             724..1944
                     /locus_tag="ACG81C_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5065780.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1941..4661
                     /locus_tag="ACG81C_002674"
     CDS             1941..4661
                     /locus_tag="ACG81C_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5065781.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4645..5589
                     /locus_tag="ACG81C_002675"
     CDS             4645..5589
                     /locus_tag="ACG81C_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5065782.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2101 a          906 c         1111 g         1537 t
ORIGIN      
        1 actatgagct tgagtatgaa aaatctattt gttcaatccc ttataataat attgaggaaa
       61 ttatagaaaa ggatgctatc agcatttaca aattttttat ttttattcct aatcgtcaat
      121 tgaaagctaa tatatttaac catttgaaaa agatgctaaa tgtgtctgtt actgaatcgg
      181 cacctgtaaa tattgaaatc gtacctcaaa atgtatctaa aggtgtggtg tttgatcgtt
      241 tggaatcaat ctacaattta aagaaacctt tgagaatcgc gatcggagat agtttaaatg
      301 acttatctat gtttgaaagt gcagatatta gttttgcaat gggaaatagt catcaagaaa
      361 tcatgaaaaa agctgattat gtcactgttt caaatgataa agacggagta tctcaagcat
      421 ttgaaagaat tcttaccata taaactaatt aaggtagtat agaacataga attctaaata
      481 cggaaaaatg gagcataaat tccagtacaa atagtacttc ttcagtgtca aacgaagcga
      541 atataacata gatatttagg attgtgacaa aaatcatgtc acaatctttt tttcgaataa
      601 acttattttt tgaagtcggc catttatcgt aatttctttg cataatacga acaaattttc
      661 ggttattgat gaaaaaaaac attgaaaatg gtatgataaa agaaaaaaag gaggaaattt
      721 ttaatggtga aatttatcca tgcggcagat ttacatatgg atcggtcatt tgaaggactg
      781 acaactcttg ataaaactgt acaggaaaaa ctattgaaga cgaatctgac cgttttatca
      841 aatatcattg aacaggcaat aataaataac gttgattttg ttttacttgc aggagataat
      901 tttcatcaaa atcgtccttc attaaaaata cagaaacatt tttcagaaca gatgaaacgg
      961 ttagaaaaga accatatacc tgtatttata atattcggaa atcatgattt ctatcaaaaa
     1021 gagcggtatt ggttctcctt tcctaaaaat gtccatttat ttacatccga aacggtagag
     1081 acaaaaaaaa taacgataaa aagtggagaa actgtctctc tcagtggatt tagttatcgt
     1141 caaccgtgga ttcaaaaaga caaagtgatg gaatttcctt ctagagaact cacagattac
     1201 catattgggt tgtaccacgg ggaacctggc gtctctcaaa aaggaaacta tgctcctttc
     1261 caaccctcaa agatgcaaga aaagggatac gattattggg ctttagggca tatccatgtg
     1321 ccaacggtct taaatgaaaa gcaaacaatt gtttatccag gtgcgcctca aggtcatact
     1381 aaaaaggaac aagcttctac atctattcta ctagttgaat taagtaaagg aagttgccaa
     1441 atacatccga ttaaagtagc agaggtctat tggaagacaa aggaaatatc attgaggaaa
     1501 gcaagaacga caaaagaggt cattgttcac attcgtcagc agctttcaaa agtagaggat
     1561 ggatttagtt taattgagat aaaattaaaa gattatgacc atctcaatac agatgttttg
     1621 gaacggatac aatcaggaga gctattagac tatttgttag aggaattctc agataggata
     1681 aatgatttct ttatttggcg tatttctctc atagagaata ctttttatac gaagacccca
     1741 cttgctgctt ctgctaaatt gatggaacag ttatttcaat actatcagac acctcaagat
     1801 ttccaacaaa ttttaaatga agtatatagt catcaagaag cggctagaat attgagcgaa
     1861 ctgccggaat atcaagaaga aacacttgaa aaagccaagc agctgcttaa tcaagatttt
     1921 ttatttgagg aggatcagga atgaagattt taaggatcga gattgctgct tttggaaaat
     1981 ggcgtcaaaa aagttttgac ttttattcag gcaatcagct gatctatggg ggaaacgaag
     2041 caggaaaatc aacgatttat caatttattc aagcaatatt atttggtttc ccttcaaaag
     2101 gccgtaaaaa aaaggattat acaccaaaag acggctcagc ctatggcggc aaaatatggc
     2161 ttaaacatcc tgtatatggt gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa
     2221 aatcgaaggt gtggctgggg gatcaagtgg gaagtgatga gttactagaa aaactgatcg
     2281 cacctttgac gaaagagtta ttccagcagg tatttacttt ccagcaagag cagttgatgg
     2341 aactagatca tttgagagaa aaagagctac atgatgcgct gatttcttta ggcatcacgg
     2401 gcagttctca agtattccaa aagcggcaag aatattatca gaaagcacaa caattattca
     2461 agaaaaaagg ccaaaaatta ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag
     2521 aaaaaattca tcaaaaacaa gaacaggaag cttattttgg caagttgatc caacaagaac
     2581 aggaatacga gtcgcaacag cagcagttat tggcacaatt aaaagaggcg aacaaacaat
     2641 tttcattagc cagacagcaa gaacttaatt tttctttata tgaagaatgg caatctctca
     2701 aaaaaaacag cactacaggt cttcctgatg agcaacttct tttagatctg gagtctttct
     2761 ccaaacggta tcagcaaaca aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc
     2821 atagcgggat ggatcagcag tcagccagat attattttta tttagagcag gagcaggaac
     2881 taaaagatct ccagcaactt caatttacag cggaacaatt aaacgaagaa tggcaaagat
     2941 tacaaattga gactagagaa acaaattcag aattgaagat tttggaacaa aggtggcatt
     3001 ggcagaaaaa tccgttaccg gaagaatttt tatatgaaga agaatggaag catcttctgg
     3061 ctgaagaaag aaggattgct gatctagaaa gccagatttc tgtgcgtctc caaatagcta
     3121 aagaacagca agagatgatt gaagcagaag tgaatgactt ggaagagacc tatccagcat
     3181 tattacaaaa tcagtcagag atagcaccag tctcttcatc taaaccgtat atgctgatgg
     3241 ctggcggagc aataatcatt gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt
     3301 tactaattgg tgcaggcatg attggctatt tcttctacaa aaggcaacca aaaattaaaa
     3361 ctaaaaatga tttctctgag ataaaaggaa tgtggcaaga aaaactagga caacttgatc
     3421 tagccttagc tcaattagct gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac
     3481 agattcttca gcaagaagta tatcgttttg cggatcatcg gcacttagga aagatggata
     3541 cgctcaaact catgaaagaa tatggacagg aagtcataga ttatcagaaa ttatatggcg
     3601 tatttgagcg gaaacataaa agagaacagg aaatacaaga aaaactacga caaatcgacc
     3661 aaaaatttga gtttgttaaa gaatggttac cgatacaaga taagacaatt tcagaaaaaa
     3721 tggtattgct ttcccgtttt atacaagaga tggaacagat gaaatttgcc agaagctatc
     3781 agcaaaatac tttattgaag caagaaatca atcaattgaa aaagaaacaa aaagtatgga
     3841 tacaagaata cagcgaacaa ttgactcaag caggcttatc ttatccttcg gaagtacctg
     3901 tctttttaca aaaagccaaa caagccgaac agcagatgaa aagatacaat gaattatcgg
     3961 aaatgcttac tccactattt cctgaagagc tgacaaaaga gaaattatct gtaagactgc
     4021 gccaactgaa agaaaagcaa tcccaactcc agcaagaaga aaaagaagca agtgaaaatc
     4081 ggcaacgact ccaacttcaa gtagaagagt tgcaaagaga cggcacattg gacgaacttt
     4141 atcaacaagc tagccggcaa aaagcaaaat tacaggaatt actggaggaa tggggagcac
     4201 ttgaaatcgc cgggacattc ttaggtgatc tttcaacaga gatgagtgaa agacaattac
     4261 cccagttgtt aagagcagca ggtgattatt taggtatttt aacaaaagga aattatcaga
     4321 agatattact gaaaaatgat acactgactt taacagacgg atatacagaa tttcccatct
     4381 atgagttatc tacaggtacg aaagatcagc tgattatggc gatgagattt gcttacctct
     4441 cattagaagc taaccgttcg atatgcccga tcatcattga tgatggctgg cttcattatg
     4501 atcatcagcg aaagtatcaa ttagctgaac ttttagctaa atttggagaa aagtatcaaa
     4561 taatttgttt ttcttctgat aaagaaatgg taagctatta ccatgagatg aaccaaatcg
     4621 taaaacaatt agaaggagca aataatgaaa aaaattcgtg atttagtaat cgatgagcag
     4681 tttgaacaat ttgtcctgat caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa
     4741 tttattgctt ttactttcca agatacttca ggtactatcg atggtaaata ctgggatgct
     4801 tcggaggagg aaatcaataa atttacagcc ggcacagtcg tttttctaaa cggtaaaaga
     4861 gaggtttatc aaggaaaccc tcaagtaaag attcttcata tgcgcttaac aagggtagat
     4921 gagccaggag agcctagttt atacatggaa agagcccctt tgaagaaaga agaaatggaa
     4981 gaagaaatca atcaattgtt gtttgagatc accaatgcgc attggaatcg tattgttcgc
     5041 tatctactta ataaatatca aaaacaattt tttgagtttc cggcagcaaa aagaaaccat
     5101 catgcatttg ccagtggatt agcttatcat actttgacaa tgcttcactt agggaaatca
     5161 atcgccgatg aatatcctga gctgaatcga gcattgttgt atgctggtat cattttgcat
     5221 gatttaggaa aggtgattga actttccggt cctatgtcga cagaatatac gcttgcagga
     5281 aatttgatcg gtcatctggt attgattgac gaagaaataa ctaaagcctg tcttgcgttg
     5341 aaaatcagcg aagctgacga agatgttctc gtattacgtc atatggtttt agcacaccat
     5401 ggcttattag aatatggctc acctgtacgt ccaagaataa tggaagcaga aatattacat
     5461 caaatcgaca atatcgatgc ttcgatgcag atgatgttga cttcgattcg tcaaacagaa
     5521 ccaggaaaat acaccgatcg gattttcggt atggacaacc gaagtttcta tgtaccaaaa
     5581 gatttatagc aaaaatttat ttttttcatt caaaacaaaa aaagcgagac ataagtcttc
     5641 gctttttttg ttttc
//
LOCUS       DAZUMI010000154         5530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000154 DAZUMI010000000
VERSION     DAZUMI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.161"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG81C_002676"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG81C_002676"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFS5065783.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG81C_002677"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG81C_002677"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFS5065784.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG81C_002678"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG81C_002678"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFS5065785.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG81C_002679"
     CDS             complement(4028..5044)
                     /locus_tag="ACG81C_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065786.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACG81C_002680"
     CDS             complement(5146..5298)
                     /locus_tag="ACG81C_002680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065787.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..>5530)
                     /locus_tag="ACG81C_002681"
     CDS             complement(5315..>5530)
                     /locus_tag="ACG81C_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFS5065788.1"
                     /translation="YEFVGMSVRKNGRLVIKYAKDNHAKRFMREENRKIDQKEFRQQY
                     EEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1452 a         1370 c          970 g         1738 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata
//
LOCUS       DAZUMI010000155         5339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000155 DAZUMI010000000
VERSION     DAZUMI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.163"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..2346
                     /locus_tag="ACG81C_002682"
     CDS             19..2346
                     /locus_tag="ACG81C_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS5065789.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2343..3257
                     /locus_tag="ACG81C_002683"
                     /pseudo
     CDS             2343..3257
                     /locus_tag="ACG81C_002683"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3330..3845
                     /locus_tag="ACG81C_002684"
     CDS             3330..3845
                     /locus_tag="ACG81C_002684"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS5065790.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3973..5271
                     /locus_tag="ACG81C_002685"
     CDS             3973..5271
                     /locus_tag="ACG81C_002685"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5065791.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
BASE COUNT         1892 a         1027 c         1057 g         1363 t
ORIGIN      
        1 tttttattga gaggagggat gaaaatgatt aagaaaactc aaaataaaat ccaacgtata
       61 tgtcaaaaac attttgtttc tagactcaaa gctgtatcta aaagaaaata cttacatccc
      121 attatgattt cgatcatctt gatttttact ttcttaggga gcttatcaag tatttatgta
      181 aacgtttata cagaaacgtc aaattttgct aaccaaacga cagaggagac aaatagtcca
      241 ccctattctg aaatacagtc catagaaagc accaatcagt ctagcaacaa cgagacaacc
      301 tcaaccacat cctcgactca actgattgat ccgacagaaa aagaaatcac agcagcaaaa
      361 gcagcagggg agaaagctta ttcagaaaca ggtagaagcc agacatttag tactgttgcg
      421 gccactgcga atttcaacgg acataccata ccagatggat atacagtgat atatgccaaa
      481 gatggagtca ttgttgcggt taacggatcg atgagtgcgg ataaaactgc gttacaaggc
      541 gtaaccaaag caatcgcttt tattgataac cgtccagata tccataatct gtttattggc
      601 tccacagtaa atggtactac tatcacattc ccaaaaagta tctatatggg ggtggctacc
      661 gctggaaata cgatcgattc agttacaccc aatgcggata aaagttattc ttttacaccc
      721 tcaacatggg gagggcttcc tgccggagga ggtatgagta ctttttcaat cggtatacag
      781 ttagcaaatg atattccatt cacggggtac tcgatcaaaa atgggcagat cgttgatggt
      841 aagggaaatg tttcaaattt tccaacctat caagcagcaa cggttcaggt caatgcgaat
      901 cggacaggaa ctttttctta tacagtcaat ggtggagcag caaaaatcgg aacgtacggg
      961 actacattat ctggaatcca tgtgggtgac aaagtggtta tcactccaac agcagtaacg
     1021 ggctattctt ttgcacaaac aaacggttct ttcacagccc tagaaagtaa cgtcgatacg
     1081 gtgacctata caggcaaaca aacaccaatc actattaagt atcaagacta ttttggacag
     1141 atgattgctc cttctaaaat aatgaatctt acttacggaa gtgctgcaca agacctgacg
     1201 actaatacgc ctactatcaa cggatatacc tttacagctg tttcagcgac agaaacaaag
     1261 aaccaatcag caatgagtgt tttagctagt ttagatacaa atggaaatgt aatcgtaaag
     1321 gacgcaaatg gaaaacagat ttctgaagtc atcctatatt ataagacaaa tgcaacagta
     1381 tcaatcaatg caaatggcag taagtactat gatggtcttt ctgttgtacc gatcgttaaa
     1441 tatgccttta atgataaaac agaatcaaca tcccttttaa acaatgatct cagtattgat
     1501 caaatcacat ggaatccaac tgatttcaaa gcgatgaatg aaaagggagt tgaagtaact
     1561 gcgcctactg aaattggaac ctatacgtct tggcaattga caaaaagcgg actagctaaa
     1621 ctagctgcca gaacaaacta tttatttact atcgtgcaga catctgatgt ctatacgatt
     1681 aagcaaatca gcggtgcggt agagctcagt ggcagtaaga catatgatgg aaaagcaggt
     1741 gtcccgtcaa tccatgtcaa gctagcagag ggtgtcacta gttcgcttac tccagtacaa
     1801 gtggcactta gcagtatgga ttacacagca gatgcacaat cagcaaaaaa tatggttaat
     1861 gctggcagct atacaattaa actaacgaat agcggaatag acaaagtcaa agcagcaaat
     1921 cccaagttgt catttactga tatagccaac acatcaggaa cttataccat ctataaagct
     1981 aacgcagtga ttactgtcga tgacgctgcc tttaattatg atgcccaaag tcactcgatt
     2041 tcggttggaa atctccacgt taccggtgtt gtcttaggcg aatcattgga ttatacccta
     2101 acagttaaca gccggacaga tattggatca caaatagtaa cgattagttt aggcgaagga
     2161 agtgtaaaca ataattatac gattatcgca acagataccg ctaagttgac gatcaatccg
     2221 actctcttgc ttccttcgac aggtgggata ggtctactgc cctttgtctt tttaggactg
     2281 atctttattt tttctggctt cttttatttt atccatcgta agaaaaaagc aggtgaacag
     2341 cgatgaaaaa aacaaaaaat gtacgtgtgg atatatttgc tgttctcttt tttatcctac
     2401 tggtcttttt tataggttct ccgactgtgt cagctagaga atcacctatt acatttactt
     2461 tgcataatcg aatgttcgaa agcgataaga tgccaagtat cagtcaaaac gacggaataa
     2521 atatcccgga taactttcaa ggagtaaaag ggttgaacgg cgttactttc gctatatatg
     2581 atgtctcgga tgaattttac aagctgcggt cagaaggaag tagtgtggaa gacgcacaaa
     2641 gaaaactagc acaaaaaagt gatagtgaaa aaatactggc agaaaatgtg acaaaaacag
     2701 tagacgagga agagggcatt gcttctttta gtgcatcgga caaagatgag caaggtcgtg
     2761 acgctgttta ccgatttgcg gaaataaaga cttcagatta agtaaaagaa aaatcagcac
     2821 catttgtcgt tgtgcttcct gtgacagatt catctaatca taaattgacg accatccact
     2881 tgtatccaaa gagcgaacaa aaaatacctg ctgcactcac attaacaaaa acagtagaga
     2941 ataagcagac agattttgca gatggggata aagttcctta tctcatcacc gtaacaattc
     3001 ctgagaacat caacgagata ggaacctata cgatcaaaga tacagctgat cctcagttat
     3061 ggctagaacc agaaacgatc gattttttga ttggtggaga caagattcat acatttcaca
     3121 cacaaaaaac aaaacatggt tttgtcttat cagattctgg aaaagatctt agttcgtttg
     3181 ctgggaaaaa attaacgatc cgttaccaaa tgacattgaa agaaaacagt gaaaaaacga
     3241 catttgataa cgatgtacgt ttgacgactg acaatcaaac agtagaaaca catcttgcca
     3301 tccaaacagg aggcaaacaa tttgtcaaag tggatcggca aacgaaacaa aaactagcag
     3361 atgctcaatt tctagtcaaa aaaaatgacc aatacttagc gcaagaaaac gggataaatc
     3421 attgggtagc caaagatgat tcaagagcaa cgatttttac ttccagtaaa gatggaacat
     3481 tttctgtcca tggattgtct tttggaagtt acgaactagt ggagattaaa ccgcctaaag
     3541 gatatatatt gagtcaatca accattctct ttgaagttga aaaaggcagt tatgatccgc
     3601 ttcatcctat accattggaa atcatcaacg aaccaaaagc aaatccgacg aataatggaa
     3661 aaacgccagt tcagatgcag acaaatggtc catcaaatga aaaaaatgga ggaagctttc
     3721 caaaaacaag cgaagagatg cttggaggtt tctcaatttt agggttgttg ctagtccttt
     3781 caacagggac agcttggttt tataaaaaac aacaaaaagg aaacaatagg agggaaataa
     3841 aatgaaaaac atcaaaacaa aaatatggac gtttttagga actgctatca tgctgcttcc
     3901 tttcgttctc ggcctaggaa ctgcagaggt ttctaggccg tatcacctac accagaaaat
     3961 gttacggtta atttgcataa attgaaattc acttcagcac cagaaaatca aataaacaac
     4021 ggaacggaat tgacatttcc gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat
     4081 gacatcactg ctacgtacta tccaagtaaa gacacagcag ttccagcaga cgctacaccc
     4141 ttcgcaagtg taacgacttc tggtgaagga ttagctaacc tgactcttcc aggtaaatct
     4201 gatgggaaag atgctgtata cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg
     4261 ccaaatatcg tgttaagctt acccttttat aacccgaatt catcagaccc tgacaaacct
     4321 ttagacacga ttcacctata tgctaaaaat gtcatcaaag aaagcaatat caaaatccag
     4381 aaagctggga acttttcagg ggtagataag ttagcagggg cgcaattcca gatcaaaaat
     4441 tcagagaatc aattcgtgac cggttttgat gaaaacactg gtgtcgctct ctatgatcct
     4501 gctgagtcag cgttgacatt cacaactgat actgaaggat ccatcagtgt caatggatta
     4561 atagacggca attatacttt agttgaaaca aaagcaccag atggttatca attaaccaat
     4621 ccacaaaaag aaactacttt tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc
     4681 atccatggcg cagatgaagc caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat
     4741 acaactacgg ataacacgat tactgtagaa aatgtccaaa actacggtga tttccacttc
     4801 atcaaacaag atgtgaacac gcaagaaaaa ttagccgatg cagaattttc tattctaaga
     4861 gcaaaagatc caactgttaa attaatgaaa aaaatagcaa atcttgcctc aggagaatac
     4921 ccatatgctt ggtcagatga agtcacaggt tctgactggg aagaagtgaa attgatttct
     4981 gattcagaag ggaaattttc aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa
     5041 acaaaagcac cagaaggcta cgcattacct aagaatgaag ctgcgtacac tgaatttaca
     5101 gtagatgatg atccaacaac ttctgatgaa acaacgatcg ataaccaacc taaaggaatc
     5161 ttgccttcaa ctggtggtat ggggatcatc gtattcatct tggtaggtac tgctttagtt
     5221 ggaggagcag tgatctactt caaaaaacgc catcaagaaa cagaagctta aactattgga
     5281 aacaaaaatc atacgataaa taaacgatag tgattcaaat ggggagaaag gttgtgaca
//
LOCUS       DAZUMI010000156         5199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000156 DAZUMI010000000
VERSION     DAZUMI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.164"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG81C_002686"
     CDS             25..696
                     /locus_tag="ACG81C_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5065792.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG81C_002687"
     CDS             755..1195
                     /locus_tag="ACG81C_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065793.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG81C_002688"
     CDS             1207..1875
                     /locus_tag="ACG81C_002688"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5065794.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG81C_002689"
     CDS             1939..2562
                     /locus_tag="ACG81C_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5065795.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG81C_002690"
     CDS             2618..3004
                     /locus_tag="ACG81C_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5065796.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG81C_002691"
     CDS             3217..4608
                     /locus_tag="ACG81C_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5065797.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAZUMI010000157         5144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000157 DAZUMI010000000
VERSION     DAZUMI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.165"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..254)
                     /locus_tag="ACG81C_002692"
     CDS             complement(<1..254)
                     /locus_tag="ACG81C_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5065798.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="ACG81C_002693"
     CDS             complement(244..1443)
                     /locus_tag="ACG81C_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5065799.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..2949)
                     /locus_tag="ACG81C_002694"
     CDS             complement(1573..2949)
                     /locus_tag="ACG81C_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065800.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2949..3605)
                     /locus_tag="ACG81C_002695"
     CDS             complement(2949..3605)
                     /locus_tag="ACG81C_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5065801.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3615..4892)
                     /locus_tag="ACG81C_002696"
     CDS             complement(3615..4892)
                     /locus_tag="ACG81C_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065802.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4911..5072)
                     /locus_tag="ACG81C_002697"
     CDS             complement(4911..5072)
                     /locus_tag="ACG81C_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5065803.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         1624 a          935 c          645 g         1940 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      421 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     2881 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     2941 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     3001 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     3061 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     3121 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     3181 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3241 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3301 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
     3361 atattatttt ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat
     3421 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
     3481 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
     3541 gacaaaactt tttctttaga atttgcataa ctatatgcta cattctttat ttctaatata
     3601 tccattattt tctcctaact tatttttgat aagatttctt taggcttctt gattaacatt
     3661 aatgaagaag caaatacaac tgataaaaca ataatactta ttaatattaa ataactttgt
     3721 ccaagtgttg ttatatttga cataaaacta ctattattta ttaaacttcc accggaaatc
     3781 attgaatcct ctgagttaat gaatccatct acaattactt ttagtaatac atttcctaaa
     3841 aataaggaag atattatact tggtatcgat atgaatatta actcgaatat aaattgcatt
     3901 ataatttgta tcttagatgt tccaatagat aagaatatac ctatttcata aattctttcc
     3961 cttaaccata gaatcaagat taatgaaaga acaaccatcc cacctaacat aatagaataa
     4021 gtcattactt ttattatata ttttattcca ctcactgact ctaaagactc ttcaaacgca
     4081 ttagaatctt tttcaacaaa atactttgac tcatcaattt taagctcttt caatttgttt
     4141 aaggctaagt ctgtagattc tgcactacca gaatacatta aaattttatt tgcaatttca
     4201 ttattttctg aattatttaa tatctcttgg cttgttgaat aatctacaaa caccatattt
     4261 tcactaaaat cagaagataa tcctgtatat gtttcctgtt ttttaccaga aaagatccct
     4321 ataattttaa atttatgact ttttattttt ccactttttt caatatctag taattcaaga
     4381 tcaacttcat cacctaattt tagattgttt tgtttagcaa attcttcatg aacaataatt
     4441 gaattcttat cattttcttc tatattttta ccttctttaa ttgtaaatac tccactacta
     4501 aataaaatat ttcttttagt attatttgta gcttcgagtg aaacaacatt cttaaattcg
     4561 tcagataaat cttctctatt tattctttgt tctccgctaa ctactttagc atcttttagt
     4621 tttgctaatc catcatattg aattattttt tcttcaattt cttttaattt ttcaatatct
     4681 ttaaattgat taacattaaa atatttacca tctttttttg ttattgatat tgaagaatta
     4741 gaactttcat ataaagcctt ttctatttca ttgcttgatt tcattattgt taaacaagaa
     4801 tacaagcaag aaagaactat agttaatatg ataaaaatta ttaaagttct atttttcttt
     4861 cttgtaatat atgctatagc attttttatc actttcctaa ctactcctct ctattttctt
     4921 actccataac taattgcatc cactggacaa gcatttttac atcgtccaca tctgatacac
     4981 tccagatgat tgcaattctc aactgaatct ttattcattt ggcaaacttt tttacatttt
     5041 ccacaattaa tacacttatc cttatcctga atcggctaca ctagactaga cagaaaaaat
     5101 aaggtgtgta gaataataaa aaacacactg gaggattttt catg
//
LOCUS       DAZUMI010000158         5137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000158 DAZUMI010000000
VERSION     DAZUMI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.166"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..2251
                     /locus_tag="ACG81C_002698"
     CDS             26..2251
                     /locus_tag="ACG81C_002698"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5065804.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            2269..4152
                     /locus_tag="ACG81C_002699"
     CDS             2269..4152
                     /locus_tag="ACG81C_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS5065805.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            4133..5107
                     /locus_tag="ACG81C_002700"
     CDS             4133..5107
                     /locus_tag="ACG81C_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5065806.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         1681 a          919 c         1141 g         1396 t
ORIGIN      
        1 tttccgtttt gttgggagtg aaatgatgga tattacgcat agaaaaacag ataaaaattt
       61 catcttattt gatgaaaaga gccgagtctt ccatttacga aatacatttc tttcttatct
      121 gataaagata gaagaaagca atgtattggc gcatatttat tttggcaaac cagtcaagca
      181 gtacaaagac aataaaaatt atccccgaag agatcgtgga ttttcaggaa atgttccctt
      241 aaatcctgac cggtctctgt cgaaagatac actaccgcaa gaatactctt ctcatggcag
      301 catggacttt cgaacgcccg catcgatcat tcagcgcaag aatggatcag acttactgga
      361 tctgagatat gacagccatt atattacaga tgggaagcca gagatcgaag gcttgcctca
      421 aacttatgtg atggacaagt cagaggcaca aacgttggtg atttcactta aggatagaga
      481 aacagcgatt tatttcgatt tgttctatac catatttact gatagagcag tgatcacacg
      541 atctgtaaag ataagaaatg aaaccggaga aacaatcaaa ctagaaaaag cggctagttt
      601 ccagctagac tttgcccata ctaggcgatt tgatgaagtc attgctttgc caggagcaca
      661 tgtcaatgaa agacagattt cacggcaatc tgttttatct ggaacaaaag tatttgaaag
      721 ccgtcgagga acgagcagtc accatatgaa taactttatt gctttagttc atcatcatac
      781 gacagaaaat actggagaag cgatcggact tcaatttgtt tattccggca accattcttt
      841 cgaacttgaa aaagatcaga tcaatcaatt gagagtcgtc ggtgggatca acagccatcg
      901 tttttcgtgg gaattgaatg caggccagtc ttttcaaaca cctgaaatga tcctttctta
      961 ctcaagtcag ggattgaaca aaatgagtca gatccaccat gagttgctac gtgaaagaat
     1021 cgcaagaggt cgtcatcaat ttgctgaacg gccgattctg gtcaataact gggaagccac
     1081 ttactttgac ttcaatagtg aaaaaatcaa ggcgatcatt gatgaagcaa aggagctagg
     1141 catcgaaatg ttcgtattag atgatggctg gtttggaaaa agagatgcgg acaattcttc
     1201 attaggagac tggttcgagt atgaaggcaa gttgacaaat ggattacgtg aaatagcaga
     1261 ttatgctcat agcaaaggat tgaaatttgg tttatggttt gaacctgaaa tgatctcggt
     1321 tgattctgaa ctgtatcgaa cacatcctga ttttcttatg caggagccag gtagaatgcc
     1381 ttctgcttcc agatcacaac atgtactgga ttttacaagg ttagatgttc ggcagacgat
     1441 tgagaaacaa atgagaaaga ttttagatac gattcctctt gactacatca aatgggatat
     1501 gaaccgttcg ctttcagatg tctattcgat tacccttgac ccacagagac aaggagaagt
     1561 agcgcatcgt tatatgctcg ggctttatga actacttgaa catttgacaa ctgattatcc
     1621 ggaaatctta tgggaaggct gttctggtgg aggtggacgg tttgatgccg ggtttattta
     1681 ttatatgccg cagtcatgga caagtgataa cacggatgca gtggagcgta tgaaaatcca
     1741 atatggaaca agccttgctt atccgatttc gtccatcaca gctcatgtat cagctgtacc
     1801 aaatcatcag accgacagat ctacttcgtt gaaaacaaga ggagaaacag ctatgagcgc
     1861 tgtttttggt tatgaactgg atttgacaaa gctgtctcct gaagaaaaaa agcaagtaaa
     1921 agaccaaatt atatcctatc aaacgattcg tccggtgatc cagtatggcc attattatcg
     1981 tttggcgagt ccttttgaag aaaatattgc cgcatggatg tttgtgtctc ctaaacaaga
     2041 tgaagctatt gtgtttttag ggaggatact agcttcagct caaccagcgt tccatgaagt
     2101 atatctgatg gggttagatg atgaggcact ttatcaggaa cagacctcga agcggatatt
     2161 ttcgggggcc gaattgatga cagttggact ttacttcccc gattttcaag gtgatttcca
     2221 aacagaactg cttcatttca aaaagttatg agagagaagg aaaaaagtat gaaagcaaat
     2281 ataaaatggt tagatgatcc acaagttttt agagtgaacc aatcaaatgc acatagcgat
     2341 catgtttgtt atgcaacaag ggaagaagta gaaaccacat cctcccttaa acagtcttta
     2401 aatggatcat ggcgtttttg ttattcgcca aatccttcgg tacgaccaaa aacattttat
     2461 aaaaaagatt ttgatgcctg ttcttttgat gaaatcactg ttcctagtca tattgaatta
     2521 gcaaattatg acaagatcca ttacatcaat acgatgtatc cgtgggaggg acatgtattt
     2581 cgccgtcccc ctttcacgac aagccctgag aaagaggcgg aagggatctt ttcagctgct
     2641 aaagacaatc cggttggttc gtatttgacg acttttcgat tagaagaagg tctaaaggga
     2701 aaacaaatct cgattcattt tgctggtgta gaacaagcaa tgtttatttg gttgaatggt
     2761 cattttatcg ggtatgccga agattcgttc actccgtctg aatttgattt aacaccttat
     2821 ttggaagaag aaaatattct agctgtggag gtttacaaaa gaagtacagc tgcattcttg
     2881 gaagatcagg actttttccg attttttggg atcttcagag atgtcttctt agttgctcga
     2941 ggacaaaacc atttagaaga tctttggatc aaacctcgct tagatgacaa atgtcaaaat
     3001 ggcaaacttg aagtagattt gtcttttgag caagtagcca aaaacagtca gctgaatgta
     3061 gcgatactag atcctgatgg gaaggaaatc tatcaacagc aatatccgtt aaaagagcaa
     3121 atgacgattc gcatcgatgg atttgaaaat gtggcactgt gggggcattt tcagcccaat
     3181 ttatataagc ttgaattgga gacgatagac gaagaaaaaa gagtaacgga gtatattgac
     3241 tattccttcg gttttcgtaa aatcgaaatc aaagataaaa tcatctactt caatggaaaa
     3301 cggatgatta tcaacggggt aaatcgacat gagtggcatc cgaaaaaagg acgcgccatc
     3361 actatagaag atatggaaaa agatttggct atcataaaaa atgcaaagat caacggcgta
     3421 cggacatctc attaccccaa ccagatcact tggtattatt tatgcgacca agcaggaatc
     3481 catgtgatgg cggaaacgaa cctcgaatcc catggctcat ggcaaaaaat gggcgcattt
     3541 gagccttctt acaacgtacc aggtgcagta cctcagtggc aggcggctgt tcttgatcga
     3601 gcaaaaacga atttcgaaac attaaaaaac catccaagta ttcttttttg gtctcttgga
     3661 aatgaatctt atgcggaaga aaacattcga caaatgaacg attacttcaa aaaagtagac
     3721 cccgatcgac ttgtccatta tgaagggaat tttccaaatc cagcattcga agattcgatt
     3781 tcagatgtaa aaagctgcat gtatgcgcca ccagaagaga tcattgacta ccttgaaaat
     3841 aaagcggaaa aaccgttcat cttatgtgaa tatatgcatg acatggggaa ttcgttaggt
     3901 ggaatggatt cttacatgac gttgcttgat cgttatgaaa tgtaccaagg aggctatata
     3961 tgggatttta aagatcaggc gatccaagtg actgatccaa tcacaggcag agaagtgatg
     4021 cgctatggcg gtgactttga cgataaacca agtgactatg aattctcagg gaatgggatc
     4081 gtttttgcag atggacaaga aaaacccgcc atgcaggagg taagatatta ttatgaaaaa
     4141 tacagtaaat aaaagtcata tggatacgga aaaagttgca atcgtcttcg gcgactgtac
     4201 attaggtgtc aaatcgggga atacgcatta tattttttct tatacaagag gcggactgga
     4261 atcgctcaat aaaaacggaa aagaatggct ataccgggaa acacttccag ttttttggcg
     4321 agcaacgacc gataatgaca gaggtagcgg atttggattt aaatccggta tgtggttagg
     4381 agccggattg tatccaaaag tcaaagaaat agaagtactg gtggacaata aaaagatcga
     4441 tttgccgatt gctccagtaa ataatcaata ttcaagtaat gaatacgctg aaacggttga
     4501 aatcattttt acactggaaa tcaacacagt accaaaaacc caagtgacaa tttcctacca
     4561 aatcgctgga gagggtaaaa tgcttgtcaa tatgcattat tatggacaaa aaggattacc
     4621 ggaattaccc gcattgggtt taaggttcat tatgccaacg aaagcaaaat tttttgagta
     4681 tgaaggccta tcaggggaaa catatccgga tcggatggct ggtggtgtcg aaggtattta
     4741 taaagtggca ggactccccg tttccccata ccttgttccc caagaatgcg gcatgcatat
     4801 ggaaacgaaa tgggtagaac taacaagaaa tcaaacgttg gataatacgg ataaagatca
     4861 agaagatttt tcgttgcgat ttgaacagca ggatacgcca tttgcttttt ccgctttacc
     4921 attcaaagca gaagaactgg aacaggcaac acatatagaa gaactaccgc cagaaagacg
     4981 aacggttctt acgatttttg caaaagtaag aggagtcggg ggtatcaata gctggggagc
     5041 ggatgttgag tcaccttacc gaatcagcag cgaagaggac catgaaatcg gctttattat
     5101 tcagtagcaa tctttagatt attatttgaa aaacgga
//
LOCUS       DAZUMI010000159         5068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000159 DAZUMI010000000
VERSION     DAZUMI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.167"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG81C_002701"
     CDS             complement(<1..182)
                     /locus_tag="ACG81C_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5065807.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG81C_002702"
     CDS             517..951
                     /locus_tag="ACG81C_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5065808.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG81C_002703"
     CDS             974..1294
                     /locus_tag="ACG81C_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065809.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG81C_002704"
     CDS             1435..1626
                     /locus_tag="ACG81C_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065810.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG81C_002705"
     CDS             complement(1697..1954)
                     /locus_tag="ACG81C_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS5065811.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG81C_002706"
     CDS             2112..2375
                     /locus_tag="ACG81C_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065812.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG81C_002707"
     CDS             2399..2587
                     /locus_tag="ACG81C_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065813.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG81C_002708"
     CDS             complement(2670..2855)
                     /locus_tag="ACG81C_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065814.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG81C_002709"
     CDS             3070..3441
                     /locus_tag="ACG81C_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5065815.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG81C_002710"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG81C_002710"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG81C_002711"
     CDS             complement(3709..4137)
                     /locus_tag="ACG81C_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065816.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG81C_002712"
     CDS             complement(4197..4955)
                     /locus_tag="ACG81C_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5065817.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1767 a          784 c          990 g         1527 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtt
//
LOCUS       DAZUMI010000160         4995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000160 DAZUMI010000000
VERSION     DAZUMI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.168"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..780)
                     /locus_tag="ACG81C_002713"
     CDS             complement(406..780)
                     /locus_tag="ACG81C_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5065818.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="ACG81C_002714"
     CDS             complement(803..2203)
                     /locus_tag="ACG81C_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS5065819.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="ACG81C_002715"
     CDS             complement(2207..2617)
                     /locus_tag="ACG81C_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5065820.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="ACG81C_002716"
     CDS             complement(2635..3126)
                     /locus_tag="ACG81C_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5065821.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="ACG81C_002717"
     CDS             complement(3136..3927)
                     /locus_tag="ACG81C_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5065822.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="ACG81C_002718"
     CDS             complement(3920..4693)
                     /locus_tag="ACG81C_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5065823.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>4995)
                     /locus_tag="ACG81C_002719"
     CDS             complement(4849..>4995)
                     /locus_tag="ACG81C_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS5065824.1"
                     /translation="HLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFE
                     QTIF"
BASE COUNT         1591 a         1015 c          659 g         1730 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatg
//
LOCUS       DAZUMI010000161         4956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000161 DAZUMI010000000
VERSION     DAZUMI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.169"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACG81C_002720"
     CDS             complement(55..828)
                     /locus_tag="ACG81C_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5065825.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACG81C_002721"
     CDS             972..1322
                     /locus_tag="ACG81C_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5065826.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACG81C_002722"
     CDS             1303..2019
                     /locus_tag="ACG81C_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5065827.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACG81C_002723"
     CDS             2019..3467
                     /locus_tag="ACG81C_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5065828.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACG81C_002724"
     CDS             complement(3537..4046)
                     /locus_tag="ACG81C_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065829.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="ACG81C_002725"
     CDS             complement(4036..4761)
                     /locus_tag="ACG81C_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065830.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1703 a          843 c          998 g         1412 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DAZUMI010000162         4930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000162 DAZUMI010000000
VERSION     DAZUMI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.170"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..971
                     /locus_tag="ACG81C_002726"
     CDS             84..971
                     /locus_tag="ACG81C_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5065831.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="ACG81C_002727"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="ACG81C_002727"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5065832.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="ACG81C_002728"
     CDS             2273..2719
                     /locus_tag="ACG81C_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5065833.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2992..4344
                     /locus_tag="ACG81C_002729"
     CDS             2992..4344
                     /locus_tag="ACG81C_002729"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5065834.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4515..4802
                     /locus_tag="ACG81C_002730"
     CDS             4515..4802
                     /locus_tag="ACG81C_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5065835.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1694 a          813 c         1030 g         1393 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatatt attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat
     2881 gaaactacaa acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt
     2941 ctgttatagt agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta
     3001 gtagtaattg gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat
     3061 ccagaagcag aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt
     3121 atcgcacttt atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct
     3181 gaagaattat cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat
     3241 acagaaaata aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct
     3301 tatgataaat tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc
     3361 gatgcgaaaa atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa
     3421 gcgaaagaag caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta
     3481 gaagcttttg tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg
     3541 aataaatatc tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt
     3601 gttactcttg cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg
     3661 aaacaagtag ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc
     3721 ttccgtccaa atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc
     3781 aaagtgaatg aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca
     3841 gtagtcaact acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt
     3901 cgtcaaggtt tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact
     3961 caaggaactt caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa
     4021 gaaagcgcaa ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt
     4081 ccagagttca tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg
     4141 aatcgtatcg taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc
     4201 atgtctttag ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc
     4261 ttccaaccac attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta
     4321 ggcgaaatcg aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct
     4381 ctgtcttttt ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa
     4441 gcagcaaaga taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa
     4501 aggtgagaaa gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt
     4561 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
     4621 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
     4681 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
     4741 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
     4801 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
     4861 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
     4921 accccttttt
//
LOCUS       DAZUMI010000163         4879 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000163 DAZUMI010000000
VERSION     DAZUMI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.171"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..721
                     /locus_tag="ACG81C_002731"
     CDS             35..721
                     /locus_tag="ACG81C_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5065836.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            723..1667
                     /locus_tag="ACG81C_002732"
     CDS             723..1667
                     /locus_tag="ACG81C_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5065837.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1866..2510
                     /gene="pcp"
                     /locus_tag="ACG81C_002733"
     CDS             1866..2510
                     /gene="pcp"
                     /locus_tag="ACG81C_002733"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5065838.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2589..3866
                     /locus_tag="ACG81C_002734"
     CDS             2589..3866
                     /locus_tag="ACG81C_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065839.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4124..>4879
                     /locus_tag="ACG81C_002735"
     CDS             4124..>4879
                     /locus_tag="ACG81C_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5065840.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1579 a          886 c         1046 g         1368 t
ORIGIN      
        1 taaaaaaata ttgtcatttt atggaggaaa atatatggaa aattatctta gccttttagg
       61 cgtcttgatt gttattctcg ggtttgcatt taaattagac tcgatattga tcgtaatggt
      121 tgctttagtt gttacagctt tgactggcgg tcttggcatt caaggaatgt tagaactttt
      181 aggtacaagt tttgtaaaca atcgcgggat ggcgattttt atcatcatta tgttggcaac
      241 aggaacgctt gaaagaaatg gtctgaaaga atcagcggct tccttgatca agcgattcaa
      301 gaaagtatct gctggtatga ttgtagatat ttatgggatc tttcgaatga tctttgcggc
      361 ttttaacgtg agttttggtg gagttgctgg atttgttcgt ccaatcattt taccgatggc
      421 attggggacc atcgaatcaa aagggttaga agtccatcct aagcatgaag aagaactaaa
      481 aggtatggct tcagctatgg aaaatgtctg ctggttcttt ggccaagtac tattcatcgg
      541 aggagcaggg gcgttgctag tccaatcaac actgaaagat ctaggatatg acgtaacatt
      601 gggacagctt gctttagtgg aagttccagt ggctatcacg gctttagttg tggcaagtgt
      661 ctacttctat cttaaagaaa agaaattagc gaagaaatat tatccaggaa aggggcaata
      721 agatggactt ttttacaaat cctgatattt tattaggcga taaattactg gagatccttt
      781 atatcgtgat gggactcgta ttgatttaca ctggagtaag aaatttaaca gataaaacca
      841 atcctcatcg ttatgggtca gcctttttct ggacggtctt aggggtagtt attgcaggag
      901 gaagatggct tccggcaatc gtgaacggtg gcttgatctt tgcgatgacc ataccagcaa
      961 ttgctaagaa agtcagcaaa ggagaaagcc ggcttccatc aaaagaatat atggaaaaaa
     1021 tgtcagacaa actaggaatg aaaattttta ttccagcttt gagtatcggt gtctttgcga
     1081 tcttgtttgc tctatttacc aatttaggtg ctttagtcgg cgtgggcgtc ggtgtttttg
     1141 cagccatgct aatcttgatg ttcttttctc gagataatcg accaagtaca tttttagatg
     1201 atgcagcaga tatgctgggc acagtagggc cgttaagcat gttgccaatg cttttagctt
     1261 ctctaggtgc ggtatttaca agcgctggag ttgggacagt gatttccaat gcagtaggta
     1321 cgatcgttcc agaaggaaat gtgaacatcg gcatcatcat ttacgctctt ggcatggtga
     1381 tatttacggt aatcatgggg aatgcatttg cggccatcac cgtgatgacg gttgggatcg
     1441 gtgcaccatt cgtatttagt catggagcga atccagcgtt aataggaatg gttgcattga
     1501 cttgcggttt ttgtgggacc ctgctgacac cgatggctgc taacttcaat atcgtgcctg
     1561 tagcgatgct ggagatgaaa gacaaatacg gagtgatcaa aaaccagttg tttattgcgt
     1621 tatttatgct agttttccaa attgcttata tgattctatt taaataatag aagagagggg
     1681 ttgtgacaga ataattgtcg caacctcttc taacattgta gtggaacact atttttttat
     1741 aagaatatat taagtttgat ctgttatgtt atttaaacta ttgtttcctt tatttacgct
     1801 gttcaaggaa aatggtatga taaaagataa ccatatgtta aatcaatcgg acggaggaac
     1861 aacagatgaa agtactagta acaggttttg atccatttgg tggggacaaa gtgaatccag
     1921 cctatgaagc agtaaaaaaa atgccagatg aaatcagcgg tgctgaaatc atcaaagtgg
     1981 aagtaccgac cgtattcgaa aaaagcagtc aagtagtcaa agaagccatc caacagcacc
     2041 aaccagatgt cgtgatctgt gtcggacaag caggtgggcg gtcagcagtc tcgttcgaac
     2101 gtgtcgcaat caatctagca gaagcaagaa tccctgataa tgaaggaaat cagccattcg
     2161 atacggcttt agaagaaaac ggtccagccg cttactttac ctcattgcca atcaaagcaa
     2221 tgacaaaaaa tgtacaagat catggtctac cagcgtacat ctcttataca gcaggaacat
     2281 ttgtctgcaa tgatatcatg tatcgtttgc ttcatttgat cgaaacacaa ttcccggcaa
     2341 ttcgaggtgg gttcatccac gtgccattct cacccgatca agtgatcgat cgtccagtgg
     2401 gtacgccttc tatgtcttta gaagacattg cttcttcttt gacttatgca gttgaagcag
     2461 cagtagaaaa taaagaagat atttccggaa atgccggaac aactcactaa tttagcaaga
     2521 aacactggct ggttgctaac gttatgttag tagccagctt tttgtaatta gaaaggaaag
     2581 aaacagccgt ggaagaatgg caatccgtct ttgaagaatg gtttccaaaa gaaatcagca
     2641 aaagctatcc catcaaaatt tctaaacaat acacaagcag tcaacgttgg gagatctatg
     2701 caaaattaac gaaaaaacag cgggaactag tcgataagca ccgccgatat ctaatcagct
     2761 ctcgttttat ggaagaacat tatttagctg caacagattg ggtgttttct gacttcaaga
     2821 tcaatccgtt ttttcgaaca aaacgaagcc agcaaaaatt gtattgtgag tgtggtagag
     2881 aattaaaagt gcaatacatc gttaaatcgc caaaaaccgg aaagatccta aaactaggca
     2941 tcaaccactt tgccgatcac ctgcatgtct ctccgacagt ggcagcttcg atccatcagg
     3001 gaatgacaaa agttgatctg gccttagacg aattattatg gcttaagcag aagaatatcg
     3061 attttcctga aggactatgg caaaaatact gttttgtctt gtaccaaaat cgtcggatga
     3121 aacagccata tttaccggat ataaaattag ctcagcggct cgcagaattt cgccaagtag
     3181 aaatgccgat ttacatagcg gattatcaag cattggaaaa tgaaatcaag aaaataagtg
     3241 aacacatcaa tgggcagtca aaaaagcgac agataaaaaa agaactattt gacgactttg
     3301 cagaagaatt agtaaaggat gtagaagaat tcttgatcaa ctatcgcgca tttttacgta
     3361 aagattggca atccatcgta tatgaagaag tgccagtaca tcctaatgct tattttgaaa
     3421 catttatttc cgtgttgaga aagacaaaac gtcaacgaac accagaagtc actgctcaga
     3481 tggaatattt tgcgaaaaac caacgattca tccaaccaaa aatctatcta tttatttgga
     3541 agcaatattg tcgctatggt tttacagaag ggttcttcga cagcattcct cggatagtca
     3601 gaaatggttt tttaaaagta ttgcgtaaag aacgagaagc tatacaatct gctgataaga
     3661 aagaccgtac agtctcaaaa gaaaaatggc aactagtagt gaaagatatc caatctggaa
     3721 atgtgcaaga gacaatcgac aaatggaaag gaaaacatta tcgttttact gaagctcaga
     3781 aacaagcgtt ggaatattat cagaagttag aagaatcact gcgtttcaat gatgaggcaa
     3841 gaaagtatct gaaggaatta ctataaaaaa catactaaga ttagagggga acatcattga
     3901 cgaatgatgt tctcctctat tttctatttt tagattatag tgaagagtga aagaaagttc
     3961 gaacaaagcc ttgaggacta cgagtcccta tcaaaaaccg aacttcttta ctgtcatttt
     4021 agaatcaggt ttacgtatgt tggggccatg agcccgtgta taggaaattg gaatcgaaat
     4081 gaagcagtaa agttctttca aaacttaaaa aggaggaaaa caaatgcctt acattcttgc
     4141 attagatgtt tcaatgggac acagctacag tgtcctatac aaagacgaca cctgcttatt
     4201 tgaagacgag attgaacaca ctcaaagagg atttcatgca ttactcgaag agatccagca
     4261 cttgcctgag cgtccattga ttgtttttga agcaacgggg atttattcca gaccagtcga
     4321 aaagttcttt caagatcatc acttttctta ctgtttgtta aacccgttag aggcgaaaaa
     4381 acagatggaa gaaagcacct tacgcagttg gaaaacagat aaatcggatg cccaccggct
     4441 cgcacagact catcttaaaa atcaacgtca gccaaaagaa gtacaaagca atttttactt
     4501 agagatgcgc gatttagccc gtttttatca agaaattgag aagaaaatca ctcgtctgcg
     4561 catggattta cacaactgcc ttcaattgac ttttccagag cttgaacagt tcttttcaaa
     4621 caggctgacc ccttacgcat tgacattgat ccgtcttttt ccccatccag attttgtatt
     4681 ggcatctact cggacaaaaa taaaaaataa actgatcaac gaaacacgga aaaaaatctc
     4741 cgcgaatcgt gccgagcaaa aggcagatca aatcatccac tatgcacagt gtgcctatcc
     4801 agctgttgaa aaagacagta tccactgtca aaaaacgatc tattacgccg aacttctcca
     4861 agatttactc gaacaaaaa
//
LOCUS       DAZUMI010000164         4809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000164 DAZUMI010000000
VERSION     DAZUMI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.172"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..1175)
                     /gene="xerS"
                     /locus_tag="ACG81C_002736"
     CDS             complement(90..1175)
                     /gene="xerS"
                     /locus_tag="ACG81C_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5065841.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1524..1719
                     /locus_tag="ACG81C_002737"
                     /pseudo
     CDS             1524..1719
                     /locus_tag="ACG81C_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3251..3769)
                     /locus_tag="ACG81C_002738"
     CDS             complement(3251..3769)
                     /locus_tag="ACG81C_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS5065842.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4136..4384
                     /locus_tag="ACG81C_002739"
     CDS             4136..4384
                     /locus_tag="ACG81C_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065843.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 ataaaaatga taatgaagct tgcctttaaa gttgggcttt tatctaattt tagagacagg
       61 cttcattatt atactatttt ttactacatt tacaaagcgt tcaaagcatc aattgtagct
      121 gcattgtcta tatgagtata taaagttgtt gtatttgtac ttgtatgtcc caattgttgg
      181 gcagttaata ccaatgaatt tgtctgttga tagagtctag tagccaaagt atgacgtaat
      241 ttatgtggac tgacttggac tctgaatcct tttgaatatt ttgctaccat ccgttcgatt
      301 gcaataccgg atatacgctt ggcttctcct tgataaattg ctagaaataa agctttttcg
      361 ttttctgtta ctttgtatcg ttctttccga atagctagat aattagctaa atattccatt
      421 gcaaatgctg ctatatttac tgcgtctctc ttatttcctt ttctagttac taccacatta
      481 ttcttttcta aagataggtc ctctaaatta atatttacaa cctcggagag tcttaatcca
      541 gatcctaaaa tcaatgcatt gatcgccagg tctctttcta catctcgttg ataatagatc
      601 aaagctcgtt tgctcaaagt ttttttatat tcattggcaa tataatctag ataattttgt
      661 gtatcttcgt ctagaaataa ttttttctcg attgcgtttg cacgggcagc taacgtagtt
      721 ttatctgtga tagttgctac ttgatccata ggattatttt ctaagtatgg ttttcctaaa
      781 ggattattgg aacttttcga aagatattta aatagaacct ttaatgctgt gatatttcta
      841 ttgatagtag aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc
      901 tcttttttta cattttcctt tgtgcgacgt ttcaaatgag atttgtacaa gtttagatca
      961 taggcagtta actgtcttat cgaggtgatt gaagtatcgg gaacgataga ctcactcaga
     1021 agccattcat aaaagagctt ataatttttt agatattcta aagtagttcg aggtgattga
     1081 tctttatcct cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt
     1141 gtatctattt tttgatatat ttctgtattt ttcatgttat tcatcctttc gataagtagg
     1201 ggagaggagt atctctataa ttggactata ccaattatag agtgttttgt caattggtat
     1261 actctgaaat ttattaattt atttcaaaaa agcagataaa taggtataga tttaataatt
     1321 taaaataaat ggtatacatg aataggaaag gaaacagaag gaggcaatct taatttttta
     1381 aattgtctaa attatataga aagaacaatc tttctatata attagacata agagagacta
     1441 cggttgattt gatattaaca agcgtatatg aatgtatata caaataaaaa aggggaggga
     1501 gaagttaaag aaaattcaga gaagtggggc actcagtagt attaacaatt taaaaggaat
     1561 ttcttaaaga aaatgggttg catcctggag atacaatttt tttagataaa gaaaaaattc
     1621 aagcaacaat tcaaaaaaat cttaactgct gagattgatg tagcaatatc tcaatcatca
     1681 acaaagtacg atcctgcctt tcaaaagctt attgataaat gtttattcat tataaaaata
     1741 aaagaaaaat ttatgtcagc ttttttcatc aaagtggtaa tgataattcg tctattaatt
     1801 gtatttttta ggaaatatgg tataattgtt ttaacgaaaa ggtaattcat gatacaccaa
     1861 aaaccactag ctggcgggct agtggttttc ttttacgttc tattcagatt aacgggtagg
     1921 cttactgtct tttttggcaa taattttgca atcaagccat ttacagatgt aatgcgcgga
     1981 tacttctgct aagattgcaa aaaacatatc gaaaaggtaa ttcataatac aacctcctcc
     2041 ccttgccata ataggccggg gtagcagaca gcataaatat tataccataa ctgaaaattt
     2101 cattttagta catttcaata tattgatgaa ccagcacaat aaaacatgcc atgactaaca
     2161 gaaaaaaact tattaaacca gatataactt gtaaacatcc aactatatag aaaacgtaaa
     2221 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
     2281 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
     2341 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
     2401 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
     2461 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
     2521 ttatgaaatg aaagaccttt tacatcttgc agaaatcgtt actgggatta aactaagcaa
     2581 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
     2641 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
     2701 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
     2761 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
     2821 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
     2881 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
     2941 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
     3001 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
     3061 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
     3121 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
     3181 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
     3241 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
     3301 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
     3361 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
     3421 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
     3481 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
     3541 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
     3601 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
     3661 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
     3721 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
     3781 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
     3841 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
     3901 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
     3961 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
     4021 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
     4081 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
     4141 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
     4201 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
     4261 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
     4321 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
     4381 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
     4441 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
     4501 ccgcgaattt actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat
     4561 taagggatca agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta
     4621 aacaattata gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac
     4681 tttcttattc tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt
     4741 acgagtacta ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc
     4801 cccacttag
//
LOCUS       DAZUMI010000165         4751 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000165 DAZUMI010000000
VERSION     DAZUMI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.173"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG81C_002740"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG81C_002740"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5065844.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG81C_002741"
     CDS             complement(1297..2133)
                     /locus_tag="ACG81C_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065845.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG81C_002742"
     CDS             complement(2317..3534)
                     /locus_tag="ACG81C_002742"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5065846.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG81C_002743"
     CDS             complement(3683..3844)
                     /locus_tag="ACG81C_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065847.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG81C_002744"
     tRNA            3917..4000
                     /locus_tag="ACG81C_002744"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4751)
                     /locus_tag="ACG81C_002745"
     CDS             complement(4240..>4751)
                     /locus_tag="ACG81C_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5065848.1"
                     /translation="YVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLN
                     NLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCS
                     RLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGIC
                     TFIITVINR"
BASE COUNT         1435 a          998 c          766 g         1552 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa t
//
LOCUS       DAZUMI010000166         4741 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000166 DAZUMI010000000
VERSION     DAZUMI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.174"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACG81C_002746"
     CDS             complement(137..1099)
                     /locus_tag="ACG81C_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065849.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACG81C_002747"
     CDS             complement(1101..2540)
                     /locus_tag="ACG81C_002747"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5065850.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACG81C_002748"
     CDS             2725..4671
                     /locus_tag="ACG81C_002748"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5065851.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1507 a          812 c          778 g         1644 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 a
//
LOCUS       DAZUMI010000167         4649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000167 DAZUMI010000000
VERSION     DAZUMI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.175"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..457
                     /locus_tag="ACG81C_002749"
     CDS             116..457
                     /locus_tag="ACG81C_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065852.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            457..828
                     /locus_tag="ACG81C_002750"
     CDS             457..828
                     /locus_tag="ACG81C_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065853.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            821..1216
                     /locus_tag="ACG81C_002751"
     CDS             821..1216
                     /locus_tag="ACG81C_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFS5065854.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            1218..1595
                     /locus_tag="ACG81C_002752"
     CDS             1218..1595
                     /locus_tag="ACG81C_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFS5065855.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1596..2204
                     /locus_tag="ACG81C_002753"
     CDS             1596..2204
                     /locus_tag="ACG81C_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS5065856.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            2204..2545
                     /locus_tag="ACG81C_002754"
     CDS             2204..2545
                     /locus_tag="ACG81C_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065857.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2787..>4649
                     /locus_tag="ACG81C_002755"
     CDS             2787..>4649
                     /locus_tag="ACG81C_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS5065858.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAA"
BASE COUNT         1664 a          591 c          953 g         1441 t
ORIGIN      
        1 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
       61 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
      121 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
      181 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
      241 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
      301 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
      361 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
      421 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
      481 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
      541 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
      601 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
      661 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
      721 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
      781 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
      841 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
      901 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
      961 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     1021 aagcggtcga attgttggag aagttataaa caatgaacat tattttaaat atgtagaaga
     1081 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     1141 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     1201 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     1261 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     1321 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     1381 tttctttctt aattgatatt gcattgattg ataataaccc taatagaaat ttaattaatg
     1441 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     1501 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     1561 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     1621 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaattttc
     1681 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     1741 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     1801 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     1861 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     1921 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     1981 gacagtttgc tctagattca gatgaaacat caacgattga aggaagtaaa gtaagtttta
     2041 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     2101 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     2161 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     2221 aacgattaaa ttaacgaaaa aagacgaaga aggtaaatat gagcaagtac aatttaaatc
     2281 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
     2341 aacagcgact gataagcaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
     2401 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
     2461 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
     2521 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
     2581 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
     2641 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
     2701 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
     2761 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
     2821 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
     2881 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
     2941 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
     3001 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
     3061 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
     3121 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
     3181 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
     3241 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
     3301 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
     3361 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggttta tctcaaggta
     3421 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
     3481 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
     3541 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
     3601 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
     3661 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
     3721 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
     3781 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
     3841 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
     3901 tgggggcaat agctgctgct aatccatttg ttttaattat tggtgcagtc gttggtgcaa
     3961 tagctgtttt cggttacttt atggcaacca atgaagagct tagaaacaag gttatatcta
     4021 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
     4081 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
     4141 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
     4201 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
     4261 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
     4321 gagaaaaatg gaatggaatc aaaggttcta ttatgtcagt tgctggtcca ttgattacag
     4381 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
     4441 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
     4501 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
     4561 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
     4621 gttattttac aaatatttat tttgctgca
//
LOCUS       DAZUMI010000168         4637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000168 DAZUMI010000000
VERSION     DAZUMI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.176"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..821
                     /locus_tag="ACG81C_002756"
     CDS             12..821
                     /locus_tag="ACG81C_002756"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5065859.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            861..1358
                     /locus_tag="ACG81C_002757"
     CDS             861..1358
                     /locus_tag="ACG81C_002757"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5065860.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1743..1816)
                     /locus_tag="ACG81C_002758"
     tRNA            complement(1743..1816)
                     /locus_tag="ACG81C_002758"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1780..1782),aa:Ile,seq:cat)
     gene            2003..2326
                     /locus_tag="ACG81C_002759"
     CDS             2003..2326
                     /locus_tag="ACG81C_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065861.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2490..2744
                     /locus_tag="ACG81C_002760"
     CDS             2490..2744
                     /locus_tag="ACG81C_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065862.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            2814..3059
                     /locus_tag="ACG81C_002761"
     CDS             2814..3059
                     /locus_tag="ACG81C_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5065863.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            3135..3500
                     /locus_tag="ACG81C_002762"
     CDS             3135..3500
                     /locus_tag="ACG81C_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5065864.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3561..4124)
                     /locus_tag="ACG81C_002763"
     CDS             complement(3561..4124)
                     /locus_tag="ACG81C_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065865.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         1460 a          824 c          779 g         1574 t
ORIGIN      
        1 aggaagataa aatgaaacta gcagcgattg atttagacgg taccttgctt gatcgaaatg
       61 gaaagatacc agaagaaaat atccaagcac ttcggaaatt tgatgaaaat ggcggaatcg
      121 taaccgtcgc taccggaagg aacagcattt ctgctaaaga tattttttcc gaattaggaa
      181 tcggtggcta tttgatttct tccaatggtt cactgattgc tgaaatgaag gatggaaaaa
      241 tggcgaaagt tctcagacgt tcaaaaatag atagcgatac actgagaaaa gccttcttct
      301 atgctcaaga agcgaaaata tccatcattg ctagccgaga aacccaagat gatcagctga
      361 cattcgatga atcaaatcta gtcaaagatg acccttatta tgaacatttc aatttacaga
      421 accattcttt tgatcaaatt ttaaatatgt tggaagatcc ttctcttagc tatcttaaat
      481 tagcattaac agataagagt gaagaaaaac tacagaagct acaaagagat ctgcaagcag
      541 atggtatcca atcagtattc tctgatccgc atttcttaga aatcacgcct acaggtatca
      601 ctaaagctca ttcacttgtt tttctggcgg attaccttgg aatcgaatcg agtgacatca
      661 tggcttttgg cgatcaagaa aacgacattg ctatgttgaa ttacagcggg ttgagtattg
      721 cgatggggaa tgcacagcga caagtaaaag aaattgcaaa ccaagtaaca ctgacgaatg
      781 aagaagctgg tgttgcttat attttaaaaa acttctatta aaaagaataa aatgacgata
      841 tgaatgtagg aggaaagaaa atgttagtta ttattgatat gcaaaatcat attcttgatc
      901 caaacagcga attttatatt ccagagtcag atcaattggc agaacgtatt gctcaaagat
      961 tagctaaagc acgccaaaat aatgaatata ttctcttcac acgggatata ccagttgaac
     1021 tgaaggacaa agaagaagaa cgagaagatt tgcaattgat ccctcttctt tctccacaag
     1081 aaaatgaacg agtgattaaa aagtattact ttacgattcc acctgaaact ttagctgaaa
     1141 tccaggaaac tttatttgaa agcaaagaag aacaaaaaga gatcgaagta gtcgggattg
     1201 aaacaaactt gtgtgtactc tctaatctga taggcctgca gagtgctttt cctgaagcag
     1261 acttttttgt tgatccaatg ttagtctcca gtagaaaaca cggagaatcc gctttagagc
     1321 tgttgaaaga tttcaacgtc tctatcgtcg aatcataaaa atatctatct tcatttacga
     1381 tatttaacac acaataaaaa aagcacgcag aactgtttta gtcctacgtg ctttttctat
     1441 caaagtagat tggctttttt atgacacaga gtgaaacggc aggctcacga cctctttctt
     1501 aaatggtgtt caatcagctg accctcggta ttagcttaac aaacggcaaa atatgaggaa
     1561 ctatttttcc gtttgttaag ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
     1621 ttaaacggta ttcaaaagct tgcatctgca gtaataagaa cagccaagta aaaacataag
     1681 aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
     1741 tatgtacctt gtaggactcg aacctacgac cggacggtta tgagccgtct gctctaacca
     1801 actgagctaa aggtacatca taaataaaat ttatttttgt tacttttcaa ctatagcacg
     1861 aatgacaaac tctcacaagt ttcaaaatat aagtaaaata atattgttat tcttatttct
     1921 tttacttgtg tcataactat tttgttactt taatttttat ttgcttattt tagaaaaaat
     1981 gttattcttt tatttattct caatgttagg agtgattatt ttgccattta ttgcgatttt
     2041 attcgattta gttgcggcag cagcttattt tctccaatta aattatcaaa cctcaggaat
     2101 tctgtttatt ggaatgattt ttcaaggtgt gattaccctt ttgcttcttg tcatgatgct
     2161 cagctataaa ggaaaaagat atgcccgtat tcaaacaaaa gtatttgtta aatatgtttc
     2221 cattcgctat gggatcatca ttctttcctt tttagtgaat gcgatcgtcc tctttttata
     2281 tgtattgaat tacttagata tcaatccatt gattttttct cgatagataa gacacattca
     2341 gcggaacagt tcctgcttat cagcagaaaa ctgttctgct ttttcatttt tcattgatct
     2401 attcttcttt tattttccta gcatttcatt tgtgatgtat gcaataacat gttatctttt
     2461 attatatgaa tcgttgagga gtccgttaca tgattacgtt ggtgagagtt ttgttttggg
     2521 tgcctgcggt cgcactggtc gcaagtattg tttatcttat gaattggaac aaagaacggt
     2581 tttatttagc tattttgact cttccggcta tctactttat gtggaaagtt tttaattaca
     2641 actactttga acccgatagt gtttttatag aagaattatc aggactggtt ttgtctttat
     2701 tgatcgtcat tctttatttg atacgtttaa ataaaaaaca ttgagtacta ttgattgcat
     2761 gtgtagcttt ctatagtcat tataactatt ctactataat gaaaggtgat taaatgagta
     2821 tcgaagaagc tattttactg gatactttac gttgtcgtct gaatttctta agaactggcc
     2881 gatctttatc tcaagtagct gtttctagcg cgttaagtat tcctttattc aaatatcgta
     2941 agtacgaaga aggagaagaa attccaagca tcattgaatt aatctatatc gctgactttt
     3001 acgatatttc cgttgattac ctaattggtc gctcagaaac agcaagcaaa ctgaagtgat
     3061 tttttccagc cttggtgctg gttttttcat ttaacgtcta aggttaacaa tctatatata
     3121 aaggagacat ttctatgcga ttatggcatg aaaaattgat ttcaaagctt cctcgtcaac
     3181 agcttttagg acaacatcgg gaatgctgtg cattaagagg aaatggctgg ggaaagaaac
     3241 atgctactgt ggattatgtt tttcactatt ctccttatag attattccaa tatcatcagc
     3301 ttgtcatgga tgaaatggaa aaacgtggtt acagcccagc accagagtgg aaaaatccta
     3361 tgtatcgtgg caaatcatgc gaagcatttt ttgatttacc tactgtagag cgcaacgtgc
     3421 cgatttatcc ggaacacgat gaagtttatc tagcagaatg tttagcaaat ttggagcaaa
     3481 aaggaattta tcttacctaa tataactaga aaagccttct atctcctctt acttcattaa
     3541 aaagctttaa ccatcttaga tcaagctaaa atgtcgtaag ttttagggtt atcttcatcc
     3601 tcacgtgcaa aactaaaagc gtactctagt tttggttcat cgccaacttc cctagacata
     3661 aatgaacatc tcctgacgtg gtttaaaagt accgcatctt cgacgtctgt atacgatgca
     3721 cagcacagat tgcttctttt gatctttctt ttgatttttc tgttgcttct tctacacctt
     3781 cttcgattac tacatcgatg cttacaattg cttctgccta atattcccat gcatcgtcga
     3841 tttaagcctc tgtagtcact gtgatcgttt ccacatttta actcctctgt cgtggcttca
     3901 gttgattttg tgatcagttc agttgctaag ccagagtcag aacaacctgc cattgctagc
     3961 gttaatattg atagtaaaaa tagttttttt atcataaggt tccccccctg aaaatagact
     4021 aaaataataa cgctttcttt ttttgttttc agacgaaaat taataatttc taaatattct
     4081 ttaaaaaagc ttataactat tatgcctttt ttaatatata acacagttat ttttaaaaat
     4141 aagaatgcta aatcactcta caataacggc gaaagcacaa aaaataaaaa aattattacg
     4201 ttattaaaaa aacagatgaa agatcaggtt ttattagatc tcattctttc atcacttgaa
     4261 gggatttaat gaactacttt ttcctctttg tcgtttttat atattcgtat tccttcaaac
     4321 agtaggatag cctctttcaa tgcttgatat ttgaactgtc cgttacttaa aaatttagat
     4381 gtgccgccct cttcctagaa catattcttt tttacttttt cattaattga cagaccaatc
     4441 gaacgaaact gtaaatttcg ataggataat gattggtcaa actcattcat tttcaagaca
     4501 tctcgattca tattcaagat aggaatcttt ttatcaacat cactttattc gtattttcct
     4561 ctaccataaa agcttcacgc tccacaaaat tgttttatac actactattc agcccattca
     4621 acgaccaatg aattttt
//
LOCUS       DAZUMI010000169         4590 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000169 DAZUMI010000000
VERSION     DAZUMI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.177"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG81C_002764"
     CDS             186..2855
                     /locus_tag="ACG81C_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5065866.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG81C_002765"
     CDS             complement(2946..3560)
                     /locus_tag="ACG81C_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5065867.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3679..4218
                     /locus_tag="ACG81C_002766"
     CDS             3679..4218
                     /locus_tag="ACG81C_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065868.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4215..4553
                     /locus_tag="ACG81C_002767"
     CDS             4215..4553
                     /locus_tag="ACG81C_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFS5065869.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1521 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa ataacggtgt
     3601 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctca aaatttaatg
     3661 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
     3721 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
     3781 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
     3841 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
     3901 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
     3961 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
     4021 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttat
     4081 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
     4141 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
     4201 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
     4261 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
     4321 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
     4381 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
     4441 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
     4501 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
     4561 atgcacagtt gagaacggaa tttgacattt
//
LOCUS       DAZUMI010000170         4553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000170 DAZUMI010000000
VERSION     DAZUMI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.178"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACG81C_002768"
     CDS             complement(12..482)
                     /locus_tag="ACG81C_002768"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5065870.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACG81C_002769"
     CDS             complement(641..1099)
                     /locus_tag="ACG81C_002769"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5065871.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACG81C_002770"
     CDS             complement(1270..2415)
                     /locus_tag="ACG81C_002770"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS5065872.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACG81C_002771"
     CDS             complement(2428..3345)
                     /locus_tag="ACG81C_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5065873.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACG81C_002772"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACG81C_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5065874.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1419 a          834 c          865 g         1435 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaa
//
LOCUS       DAZUMI010000171         4517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000171 DAZUMI010000000
VERSION     DAZUMI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.179"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1491
                     /locus_tag="ACG81C_002773"
     CDS             190..1491
                     /locus_tag="ACG81C_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5065875.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1518..1778
                     /locus_tag="ACG81C_002774"
     CDS             1518..1778
                     /locus_tag="ACG81C_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5065876.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2777..4012)
                     /locus_tag="ACG81C_002775"
     CDS             complement(2777..4012)
                     /locus_tag="ACG81C_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5065877.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1328 a          705 c          911 g         1573 t
ORIGIN      
        1 tgttaattat cgaatttttt tgttgctcct atgatttaga aaacttattt tcaggattct
       61 taaaaataaa tttgattatt taagagaata tattgaaata aaatttctta attatataat
      121 tagttatgaa cattctttgc gttgtcatgc aatgaatggt tcatatttta ttgaaaagga
      181 gtggttttag tggaaaagct gatgaaatgg gtcgaagaga aacttgtccc accaatggca
      241 aaaattggta cacagcgtca tttattagca attcgtaatg gggttgtttc aacattgtca
      301 ttgattttga ttgggacatt ttttatggtc tttatcaatt taccgtttcc tggatggaat
      361 gaatttattg ccccttattc ggcaacgatt gttttaccct ttaggatgac gatgggattg
      421 atggctattt atgcaacttt tgttatgggt tcggatctag ctaaatcata tggattagat
      481 agtgtgacag gtggtatatt atctttagga acgttcttta tgttacaagt tcctgtaaac
      541 gttttaactc ctgaagaggc acctcttggc tgggtcttac ctatgagttc tttgggagca
      601 tctgggatgt ttgcggggat cttatctatg atttttgcag tggaagtata tcgtttcttt
      661 aaaaatcgaa atattactat caaaatgcca gaacaagtgc cacctgctgt tgcccgtagt
      721 tttgaggcat tgattccagg tgcagtcatt ttaacaatta cttggctgat tcgaagcgtc
      781 gcgggctttg atgtaaacgc tgccttatta tcgttattcg agcctttaac caatatttta
      841 gggaataatt tgcttggtgt tttactacca atgtttttga tccatttgtt gtggtcattt
      901 ggtatccatg gcatgagtat tgtaggggct gttgttcgtc caatgtggct gatcatgttg
      961 gatgagaatg caaaggcatt ggcagatggt acagctgcca cccaattacc ctatatcgct
     1021 cctgaacagt tttatcaatg gacggtaaca attggtggtg caggagcaac gatcgtggta
     1081 tctgttttgt ttatattctt ctgtaaatca aaattcttga aagaggttgg tcgtttttct
     1141 attattcctg gtatcttcaa tatcaatgag cccataattt ttggagctcc tatgatgttg
     1201 aatccttaca tgttcatccc gttcaactta gttcctttag ttttgactat tatcagttat
     1261 tttgcagtca aattagaaat ggtcaataga tttactgttt tgccaacatg gacattacca
     1321 gctccaatcg gcggttatct ttcagcagga aatgactggc gtgttgttat cttgatcatg
     1381 atcaacacat tgatagcatt tattatctat tatccatttg taaaagctta tgataaaaag
     1441 atgctgtcag atgaacaaag tcgtggagaa aacgaagtcc agactgtcta agtttggttt
     1501 ttagaaggtg ggaaacaatg tattggctga ttttattttt tgtttttatc tttttattaa
     1561 cggctagtca ccttatattg aatatgctag ctacatatca tattcaaatc aatcgatgga
     1621 tttgggcact ggcatcattt ttgatcgtga ttcttccaaa gatcattgta ccgcatatga
     1681 atgttttgtt ttcatggggg acatatgtac tttgtggaat ttttgctatc aattttatga
     1741 tcgagcagca tagatggttt gtcacatcga agctttaaat gttctaaaag tggttgtaac
     1801 agaagtggtt agcttcaaga aataagaaga aatttacgaa aattgcttct taaatttttg
     1861 tgaaatgttg gcttattttt cgtttggttg aacttattac cgcagatgca agcttttgac
     1921 caccgtttat tcggaatatg ggactgtgac aaggcttttg tcacagccct tttttacata
     1981 aagattatta ttgttttaac tgttttggca gaaagaagga ggaaaaaatg gaaattttat
     2041 cagcaatgga tttagaatta gtagatctac taccaaagtg atcaagtcaa atatagattt
     2101 gggagcttac aaccatatag taggggctgc agtatggtgt atgaatggta atatgtatga
     2161 tgtgagtgtt tattcaaatc atgaaacgtg tatagaaata attgtgctgg gaatagcaat
     2221 cagtcatggt gaacgtgact tttagtgtat cgttgctgac gacatagaac attctgacat
     2281 gatctactca atatgtgggg attgctggca gtttatatag gagtatgcat cggattgcga
     2341 gatcattgtc tcaacagaag agggaggttg attatcaaag ataaagtggt aaatcctaca
     2401 tttgatttag taattgttga ggggactagc ggcacaaatc tctacaaagc gatgcttcta
     2461 gatctatatg acacatttta agaaaatgta tttgcctaat tattatggtc tttcttttga
     2521 agaaacgttg agatgatcca accagagaaa ggtgtcaaag aatttgacta agaataaatg
     2581 gcagacgcta cctatgctca tttggatagc gtcagatctt ttattttaag gatgattttg
     2641 tgttttttag acaccgtaat ttaagtaact ctaacttttc aatccactat ttactttttt
     2701 tgtattttca ctgtctgggt ctcgaatagc gaaattactg atgagtctct attaaaaaag
     2761 ctttttctac ttgaaactat attgggtggt tgctcaggaa ggtttcgatt tcttgaattg
     2821 cttgttgtgg accatttatt ggaaaaacgg cttgcagcgt gcgtgcctgc tgacgaatct
     2881 tgggtgaaga aacttcgcgc agagcaatct gcagttttct ttcagtcact ggttttgtga
     2941 gggcgattgc gtttccgtat tttacacaat agtcaatatt acagctttgc tcaaattgca
     3001 tccccacacc gataaacggt ttgccggatt cacaggcagt ttggacggtc ccttccccgc
     3061 cgtggataaa agaaaaatca atgaattcat gcaaaagatg actagacaga tattctgtta
     3121 ttaaaagatc ttctgagtca gattgaatgt catcaagcag atagtcaata ggtgcaataa
     3181 ccgctagatc cggttgtttt tgtaagaaga acaaaatttt gcgtaacata cgcggattgc
     3241 ctgaactacc cattgcgaaa tagataatac gttttctttc ttgttgcttt cgctgtataa
     3301 aagttagcac ttcttcaggc aaggaagtac tgagcttagc aaaaattgga ccaacgattc
     3361 gataattttc cggcagctgt tcttcttcca aaaatagttt aggcgttgtg atgagattta
     3421 gatcagcatc catcatttgt aaacttgttt ttggtaaagg aagcatttct ttttgtgcag
     3481 cgtgtttgaa ggcattgggg atccatcggg tatttactat acgctttttt gtccagtgcc
     3541 aaaggggatt gcttaaaggg ccgaagaaac gtaactcgtg gggcatcagc gcatgctctg
     3601 attgaaaaaa aggttcactc aaagcatagg gtttaacata tactaaagga atctttttat
     3661 tgcgggccga taaaaaaatt gtcacattcg aacctgtcac aatcagtcga ggctcaaact
     3721 tagcaatcag actctgttca ctagtgatac gagaggcaac catttctttg gtaaaagggt
     3781 ttttaagtgt acgcaactgg tcaaatttta tgatttgttc ttcttgtttt ttcgtaagga
     3841 tcggcgctag taaatgatac tcaaagccac tttctgtgat caaaggagca aaagttttag
     3901 aaaagccaaa gaatacacag taataacggt ctttcatagc tttggcaacc tcaatcattc
     3961 gtgttgtctc agctaaattg aatactgcag gagcaaaaag aattgtcttc atgaaatcct
     4021 ccttattgga atgagtctat cccttcgttt cttactagta tactaaaaaa cacagcgttc
     4081 gagtagccta tgtatatttt gaatattaaa ctgtctctcg ctgttttcac aacttctaaa
     4141 tttaaatttt gtaatcaagt tgaaactcca attttcaaaa aaacacgcaa caattttttg
     4201 atcgatttat atgttgtgcc agataaatac gagaagttgt gtcattaaaa ataaacatga
     4261 gaacataaaa aaataaaata attatattaa ttgataggtg tgctgtcgtc attatacaca
     4321 cgtaagaaag aaatagttat gtttaaaaat attcttagac tttctcgcca tgacaatttt
     4381 cactatgtgt tataactgta aacgataatt attcagataa cacaaaaaga aatcaaggag
     4441 cagatacgcc agttgtttaa tttgaatatt attttaaaat taaaattttt attctgttct
     4501 aaacattttt ggttgtt
//
LOCUS       DAZUMI010000172         4486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000172 DAZUMI010000000
VERSION     DAZUMI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.180"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG81C_002776"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG81C_002776"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5065878.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG81C_002777"
     CDS             complement(1036..1956)
                     /locus_tag="ACG81C_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5065879.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG81C_002778"
     CDS             2069..2821
                     /locus_tag="ACG81C_002778"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5065880.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="ACG81C_002779"
     CDS             3685..4218
                     /locus_tag="ACG81C_002779"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5065881.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1490 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAZUMI010000173         4322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000173 DAZUMI010000000
VERSION     DAZUMI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.181"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..558
                     /locus_tag="ACG81C_002780"
     CDS             121..558
                     /locus_tag="ACG81C_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065882.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            653..1537
                     /locus_tag="ACG81C_002781"
     CDS             653..1537
                     /locus_tag="ACG81C_002781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5065883.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1759..>4322
                     /locus_tag="ACG81C_002782"
     CDS             1759..>4322
                     /locus_tag="ACG81C_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5065884.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT         1411 a          724 c          965 g         1222 t
ORIGIN      
        1 tgatttagtt ctttttattt taaaaatttt ttttacttag aatccagcca ccctagatat
       61 ttttctgtat aatcttaaat aaagaaaacg atattaaaaa atgcacgtag aagtagataa
      121 atggagtggt tttgcaaatt tcttttattt ctagaagtta ttcacgtata cggatttgtt
      181 tttctcacaa ataaaaaggc aggaagtaca atggatcatt tgtatataaa atatattcat
      241 caattttccg ttttggtatt aggcacacta attatagcgg ggattgttgc atttattatt
      301 caaggtcgtg acatattaac agtacatcaa gagtttttca gttatgcatt tggtattcct
      361 ctgatcgcta taaggtctga taacaaaaag tggaagaaac gctattacat tctcttgcta
      421 tttttgtgtt tttatctgcc acctaagatt tttactttat taggtataca atgcctatca
      481 ttttctttta tttcctatat cattgtttta attttcctca gtttcatagt atgggtattt
      541 tatcttgtca aaaaataaat gaatgtttca tgaacagtac ttcatcacag cctaattttt
      601 ttctttatgc tatcgttata ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa
      661 agttttatta gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt
      721 agcgatagca aaattggaat cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc
      781 aggggatgca aaaaacttta ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt
      841 catgggcgga gatggaacag tcaacgaagg aatcagtgga atcgctgaac aagaacatcg
      901 gccaaatttc ggatttttcc cattgggtac ggtgaatgac ttggctcgag cattagggat
      961 accgctagaa cctgaggaag cgatcaatca tttttcgatt gaatcagtca agccattgga
     1021 tattggaaaa ataaatgacg actattttat gaatgtcgtc gcaattggct ctatccctga
     1081 agcaatcaat gatgtagatg cggagaagaa gacgaaattt ggaaaactag cttatttcat
     1141 gtcaggaatc aaacagttag cttccacaca atcctactcg tttcacgtag aagttgatgg
     1201 aaagaaggaa gaaattgaga gcagtatctt gctgatagga ctaactaact cagtaggcgg
     1261 ttttgaaaca ttacttccaa atgcaaaagt ggatgatggt aagctccatt tggtttattt
     1321 aaaagattct tctttgatcg atacgcttaa aacattgcca gacctattaa aaggagtaga
     1381 tgaatctacg aaaaatctag cctatcttac ttgtgagcaa ttagttgtgt cattagctga
     1441 tgaggaagaa ttgacgacga atgttgatgg agatgaaggg gataaattac ctgtgacaat
     1501 caaagtgtta ccctcacatt tacaggttta ttgttaaaat ggaggttgtg accgaagagt
     1561 tgtcaggagg acatcggttg ttcggaataa gggactgtga caagactttt gtcacagcct
     1621 ttttttgtac aaaaaagaaa ttttcaccta acagaaaaat ttgccataaa aatacatatt
     1681 ttaaatatga aagtacaatt acaaataaat taaaacgtta aaataaaatt gtcaaaacgt
     1741 cagagaggat ggtggagaat gaatcaatat gtaaaaaagc tgattgcgtc gattttgttt
     1801 gtcttccctc tcttgatggt gacatccgtt tttgccgatg tagagaaaac aagcgaatca
     1861 acgcaatcag agaatcaaag ttttgacaag actacttttt cagaagaatc aattacttct
     1921 gaaactacag aatcccaaag tgaggcgcct acttctaata caaggcaaga agaaaataag
     1981 acaagtgaga cctcagaagt agctgaacaa acgtctgata gcagtgaaga ggtacaggtt
     2041 agagttgttt cagcggatgg gtacagcgaa gctgccacaa cagaagaact gacgcagctt
     2101 ctcgcagacg aatcagtagc taagattcgt ttaactcagc ctttgacttt agacagggaa
     2161 ttggagatca agcgagatat cgttatcgat tttggcggac ttgctcataa tttcggcaca
     2221 catcacatct atatcaatga gacacctcag tcgatcgagt ttcagaattt taaaggaacg
     2281 gcagctcatc caggtgggtt gataccaaat atcgatggaa atgccattat cattgcttat
     2341 atgtccgatt tttggggaaa cagatatcat tttacgggag aaataaaatt taccgggaca
     2401 cttgacttat atgatggttc taaattaggc ctgatttacg cccctcgtgc aactgtcaca
     2461 ttagatggtg tttccggtgt attggatgtc caaccagtga aagaagggat gaatgccgct
     2521 ccgggtaaag cttatttttg tagaagctat cagctaaatg taatcaatgg tagtcagctt
     2581 tatggaccat atctaggaaa attctatggt tttctagatt caggtgatga gacgggcagt
     2641 ggaaaaacag caccaggact ccatatcttg agtggttcaa aggtctctct tgactatgat
     2701 cgggcagatg gcgtcagcga tgaaggagag gctgtcgata cgctacccgg gaatgtcaca
     2761 tttaaagtca gtggctcagg aagtgaattt tcggtaaata caaagatcaa tattaccaac
     2821 gacaacaatc gcggaattat ccaaatgaga ggctcaggat caagagtcgc tgtgtcgaat
     2881 gatgggaaaa tcacaatcaa tacagcgact actggcggat ttcgcctgca aggagattct
     2941 agtcaaatcc atgtatcaag tggtggacaa atctcggtca aaatggctgg agatggcgac
     3001 caacctggaa acaatgggat gcgttttgta gggaaaggac tttctttgat cgtagaagga
     3061 gccggatcag tcatcgatat tgataagcaa agtggtcgtt ctagcgcagt tcgttttgag
     3121 aacggtacgc aaaaactaac agtaactaat ggaggctcat tgaaggtaag aaatgcaggg
     3181 gatggcaaat cttatgtgaa cagactcaat caaggaaacc aagcgatcca attttatgac
     3241 gcttctggtt ttctagaaag tccagggtca gctgatttta cggttactgg agaaaaatca
     3301 aatatcgata tacgcgcaga ttccggagct accgtagata caactggaga tattgatttg
     3361 aactttatgg ctggcgaaaa aacttattta gtcatggtcg ggaaaactgg ggaagataag
     3421 actggcatca tcagtgggga tgtgttgaat gtagacttag aatctcctac ctatttcgat
     3481 ttccaaaaca aacggatcgg aagcgagact aatggcatag gtggctgggt atttcaagga
     3541 aatggcgatt cttctttgaa gatcgctcat tcagaaattg ctttatggcg aaaggcagga
     3601 gtaacgttcg ataactttga aaaagatccg gattattttt ctgatatgac agacttatcg
     3661 atcaatggaa ccgacttagg taattttgta tcaagcccta gtgcaggttt aacaaatgaa
     3721 tttgctcaga caggtgccgg aatgaaaaat tataatcgaa tcagtgccaa taaccagctt
     3781 ccgatgattg aatccttaag agtaccaacg aatgcagata aaaaaatcta tgggcatgtc
     3841 gttgttcccg aaggtgtcga ggcaaagcca agagatgctt ggacaggcga agtagaagtg
     3901 actttacgta ttacttatgc agatacgacg aaatcacctg tagaaattac tgctctcaca
     3961 caaggacaaa cagatgaaaa tgaagggaca ggatataatc cgtggggaga aggcgaacaa
     4021 ggcggtttgt ttgaggttca agtacccaac gaagaaagac taacaacggg ggatcaagtg
     4081 caaatagttg ctgctaaaaa agtaagaggc ggccaagaag tgacagaact ggactctgcc
     4141 aaaacgacag tcgatgtgat tcctcctgag cctgctaaat taagtaccac attgatcaat
     4201 gcagcttctc gctcaatcac aggtacaggc gaagcaggag ctttggtttc attgaaaaaa
     4261 aatcaagctg ttttgtccga aacaactatt gatgatacag gtaaatttgt tctttctatc
     4321 cc
//
LOCUS       DAZUMI010000174         4189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000174 DAZUMI010000000
VERSION     DAZUMI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.182"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..825
                     /locus_tag="ACG81C_002783"
     CDS             127..825
                     /locus_tag="ACG81C_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5065885.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(993..3632)
                     /locus_tag="ACG81C_002784"
     CDS             complement(993..3632)
                     /locus_tag="ACG81C_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5065886.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1203 a          912 c          794 g         1280 t
ORIGIN      
        1 tactttattt aatattcttt tttttatcct ttttttatga ataacaaaaa aaagaaaaac
       61 attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga aaaggaggga
      121 aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct aaaaggacgt
      181 tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc tactattctt
      241 cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc agcgagcgtc
      301 tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat tatttccagc
      361 atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat tttacgtgga
      421 caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg tttgtttatc
      481 ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg gggtttgcta
      541 ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt tatttactac
      601 gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac tgcaagtcga
      661 aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt tatcggctgg
      721 catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc atacatttct
      781 gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag agtgtgactt
      841 gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag aaacattcag
      901 cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt gtatagttcc
      961 agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc ttccggtaat
     1021 cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat ccctaccaag
     1081 aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa tggcagcaaa
     1141 gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc tttattggtc
     1201 gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg cagaatcgat
     1261 gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc gtctacccat
     1321 gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac gatggtgaag
     1381 acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc ttcatttttc
     1441 ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa agcaaatcct
     1501 ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa tggtgctccc
     1561 catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca gcttagcaaa
     1621 aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac agcgtctacg
     1681 atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc cacatctgtt
     1741 cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc atcattcaca
     1801 ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac gatccggatc
     1861 ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc atctaattct
     1921 tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc attcaagata
     1981 cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat catgactgtt
     2041 ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg tggtggatcg
     2101 atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga agaaatatct
     2161 tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc ccgtttacca
     2221 aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg cggagcaaga
     2281 acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg aactgttgtc
     2341 atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt ttcttctaat
     2401 tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc tgttggatta
     2461 cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca tagtgcagcc
     2521 attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt tacttcatct
     2581 tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc tgttttgtct
     2641 gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg gatgatcgca
     2701 tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat aacagtcaag
     2761 gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc aaggagtggt
     2821 tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc gatcatactg
     2881 attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat agtattttcg
     2941 ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt gacgatcgct
     3001 ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg gtcacctaat
     3061 gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact ttctccagtc
     3121 aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc ggcagaaata
     3181 gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga ctcaacagtg
     3241 gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg caaggattcc
     3301 agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat cacaacgata
     3361 gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc ggtataaaag
     3421 gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct tcgaaagaat
     3481 tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg cttttttcgc
     3541 cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt taccttcttt
     3601 acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa tcttcgtaca
     3661 aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat aaagggctta
     3721 tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa cgctaccaaa
     3781 ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga agaatttctc
     3841 gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca gacatgcgtg
     3901 tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga agtatccgaa
     3961 acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa ttgaatagga
     4021 acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt taacatgaag
     4081 aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct gtttatttga
     4141 atccttccga ggtacaagaa agccgagctt atcgagagct cggcttttt
//
LOCUS       DAZUMI010000175         4069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000175 DAZUMI010000000
VERSION     DAZUMI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.183"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACG81C_002785"
     CDS             85..1434
                     /locus_tag="ACG81C_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5065887.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACG81C_002786"
     CDS             complement(1562..2152)
                     /locus_tag="ACG81C_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5065888.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG81C_002787"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG81C_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5065889.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAZUMI010000176         4002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000176 DAZUMI010000000
VERSION     DAZUMI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.184"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..563
                     /locus_tag="ACG81C_002788"
     CDS             <1..563
                     /locus_tag="ACG81C_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5065890.1"
                     /translation="NVLDRTFTQEQPLEAIVTDLTYVRVRKKWHYICLILDLFNREII
                     GYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDSQTIHEILNGFGITRSLSR
                     KGCPYDNAVAESTHKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLCLHGTLAYETP
                     IQIRQQRLAKRILDNERGSDTSGEAA"
     gene            669..884
                     /locus_tag="ACG81C_002789"
                     /pseudo
     CDS             669..884
                     /locus_tag="ACG81C_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1519..2478
                     /locus_tag="ACG81C_002790"
     CDS             1519..2478
                     /locus_tag="ACG81C_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5065891.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(2465..3070)
                     /locus_tag="ACG81C_002791"
     CDS             complement(2465..3070)
                     /locus_tag="ACG81C_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065892.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(3310..>4002)
                     /locus_tag="ACG81C_002792"
     CDS             complement(3310..>4002)
                     /locus_tag="ACG81C_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5065893.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT         1301 a          682 c          670 g         1349 t
ORIGIN      
        1 cgaatgtatt agatcggacc tttacacaag aacagccatt ggaagccatc gttacggatc
       61 ttacttatgt tcgcgtgagg aaaaagtggc attatatctg cttaatactt gatttgttta
      121 atcgagaaat tattggttat tcctgtggtg agaagaaaga tgcctcattg gtaaaagaag
      181 cctttggacg gataccgtat tctttaacag acgtcaagct ttttcataca gaccgtggaa
      241 aggaatttga tagccaaacc attcatgaga ttctgaatgg ttttggaatt actcgttcat
      301 tgagtaggaa gggttgtccg tatgataatg ccgttgcgga atcaacccat aaatctgtca
      361 aagtagaatt cgtgcatcaa taccaatttg agacactggc acagctacgt ctagaattgt
      421 ttgattatgt gcattggtgg aactatctat gcttacatgg cacgttggcg tatgaaacac
      481 cgatccaaat tcgacaacag agattggcga agcgaatcct tgataatgag cgcggatctg
      541 atacctctgg agaggcagcg taattgaatg attgtgcttc tgccggagaa aatcagatcc
      601 gaggatgctc attgtcaagg gcaatcggag ccataacacc gcagcattca caacacctta
      661 taatttttgt caaaaaaagt gttgccattc caaccaatct aatcgagtct ttcaataagc
      721 aaattaaaag atacagccgt agaaaagagc agtttcaaaa tgaagaatca ctagaacgct
      781 ttctagtcag catttttgat acatacaatc aaaaatttct aaacagaagc cataaaggtt
      841 ttcaacaggt aaccgataca ttagtttcaa tgtttactga gtaactaatt attttgcagg
      901 aggacaattt atttacacaa aattattgac gctcccttaa caataaatga agactttttt
      961 attgttaatt gtttattttt ctattattag atatgaaaat cgcatttaga taaaatagaa
     1021 aaatatgaat atattttaaa tattaagatg agagtcatta acttatgata gtagtcaatt
     1081 tgcttttcac taattacaaa atatgtgata taggaagttt agttattgtt ctcataatgc
     1141 catcctgttt ttagattatc tagtaatttt tttgttattt aaaattagga gcagtgccaa
     1201 ttgttttaat tttaaaggct ttttttatat cttctaaatt agaagatatt tgtatatcaa
     1261 tatggaatat attacctttt ttgtattttt ttatgttatc tttagctaaa acatcaaata
     1321 catgtccttg aaattgtatc gaggcagctg gatttagttt aaattgagga aactttacca
     1381 cagctgattc aaaacacttg tcacattttt ttattttttt accttcgccg attgtaaaat
     1441 taagctagac aactaaaaaa gtcatttgtg ctacactcaa aatagttccg tcgaaagaat
     1501 tataaggagt gagtacaaat gacctatacc catcttacac caactgaact cgtaatgata
     1561 gaagcttatt tcaatcaatc acactctgtt tcaaaagtag cccatctggt acaacgttct
     1621 cgacaaacca tttataatgt ctatcgtttt cttaaatctg gtggaagcgc aataagctac
     1681 tatgaacaat ataagaaaaa caaacgtaac tgcggaagac gtccgattgt gttaccagat
     1741 gggcaacaag aatacattca aaaaaaggtg gctcaagggt ggactccaga tgtccttatt
     1801 ggtcgagcag agtttcctat tgcttgttcg gtacgtacac tatatcggat gtttaaagat
     1861 aaaacctttg accctcacga tttaccaatg aaagggaaaa gaaaaccaaa cggacataaa
     1921 gaaaaaagag gaaaacaagc ttttcgccgt tcgattcagg atagaacaac tgattatata
     1981 cagttcaatc atgagtttgg tcacctagaa ggtgacacca ttgtcggtgg aaaacataaa
     2041 agtgcggtga tcacgttagt tgagcgatta tctaaagtga tcatcacctt gaaacccgct
     2101 ggtaggcaag ctatagatat tgagaataga ctgaatcaat ggtttcaatc tgtaccgaaa
     2161 cacttgttta aatccatcac atttgattgt ggaaaagagt tctcgaattg gaaaaagatt
     2221 agtaatacca atgatatttc gatttatttc gctgatccag gaacaccttc tcaacgagga
     2281 ctaaatgaac attcgaatgg cttattacgt aaagatggct tatataaagc tatggatttt
     2341 aataaggtat ccgaaacctt tatccaatcg gtggcatcaa aacgaaatca tattcctaga
     2401 aaatcattac actatcgcac accattggaa gtatttttga gttacgtagg taaagatgat
     2461 ttgtctagct taatttgaca aacgaaactt tgtaaaaata ttcgattgaa tattctattt
     2521 ggcacgactc acttggcagt atatgtatgt caatattttt catctgtttc ataggtattt
     2581 caaaatctaa actattaaaa acatataatt ccctgtaatt atttacatct aactctacta
     2641 aatttatctt tggaacaata gatcctccac ccattccacc ataataaaaa aaatcttgtt
     2701 ctctatcaaa atagcacata ttaagctcag tttgtttatc atcctcaata attttataaa
     2761 tcaaacctat ataatcatga atgctggcaa aaattttttt taagtcaagt tctaaatctt
     2821 cttcgaaaac acatttaatt tctcctactg ataattgata ctctatatta catgacttaa
     2881 ttagtttttt agatcccccc tccagatagg catatgtttc aataattatg ttctttttat
     2941 tgcttggcaa atttccgtta tttattgcat aaattaaaaa tgactgtcta tttagcccat
     3001 aatctaatac aacttcttca tactcatatt tctttaaata tttaatatga aaatatgctg
     3061 aattaatcaa aactcgagtg ttactgttgt tatttattgt tgttctattg tgtataaaat
     3121 tgtataggtt gtcatatttt cttggtttag tgaactactc accacttagc taaacctaac
     3181 ggtttcttac gtttgaagtg ggagcttctt gggaagagag catacttgat agcgtattac
     3241 tttactaaca gaggtgcctc ttatttacca agctatcccc gtagttccta cggttcttgt
     3301 ttatttgatc taagccaatc ccatagagtg tagccttaga ccttcggtca ggatattgat
     3361 actagcgttg atatctcggt catgatgagt atgacaaata gggcaagtcc attttcgaat
     3421 ttcgagagat ttcttgccgt ctttatgtcc acaattcgaa cagatttggc tagatggaaa
     3481 ccacttatct actttgatga ttttccgacc gtaccagtca gccttgtatt gtaatttaga
     3541 cacaaaacta gaccaagaaa catccgaaat actttttgct agtttatgat tacgcaacat
     3601 gccttttgtg tttaagtctt caatacagat gatatcgtga tttttgataa tttctgtact
     3661 caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc gtgctacttt
     3721 acgtttttgt gtttgataat ttctagcttc aaacagattg ataccttttt gtttagctaa
     3781 taaggcacgt cttgacaact tacgttgttc acgtttgagt ttcttttcca ttttggacgt
     3841 aaatttatta ttatcaaatt tttgaccggt agaaagaatt gcaaagtcta taatacctag
     3901 gtcaatacca acagcagaat tagatttagg tagttcgcga acttcttctt tgcataacaa
     3961 agaaatataa tatttaccgc tagaatgacg tgatattgta gc
//
LOCUS       DAZUMI010000177         3978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000177 DAZUMI010000000
VERSION     DAZUMI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.185"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1593)
                     /locus_tag="ACG81C_002793"
     CDS             complement(88..1593)
                     /locus_tag="ACG81C_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5065894.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1590..2276)
                     /locus_tag="ACG81C_002794"
     CDS             complement(1590..2276)
                     /locus_tag="ACG81C_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5065895.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2469..3890)
                     /gene="gndA"
                     /locus_tag="ACG81C_002795"
     CDS             complement(2469..3890)
                     /gene="gndA"
                     /locus_tag="ACG81C_002795"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5065896.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1096 a          848 c          684 g         1350 t
ORIGIN      
        1 ataatgacat ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata
       61 tataaaaaaa caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa
      121 gattctaaaa attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt
      181 ttcaaccagt tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct
      241 tgctttatcc actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc
      301 ttcgccaaag tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg
      361 gacttctttt cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg
      421 ttcaaagtga ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt
      481 gatcacatag tctggatata atatcttgaa attattataa acttgatagg tgacttcttt
      541 tcctttagtt gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag
      601 catctcttga actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc
      661 ttctaatatc tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat
      721 aattgcaaca ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc
      781 aatatacaaa cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc
      841 atcatttgtg tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc
      901 cattgtatct ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa
      961 aatactactt aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt
     1021 tcgaatatca tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct
     1081 tgtaccttta gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac
     1141 gatcgttgga actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa
     1201 aatcaagttc tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc
     1261 tataaaggaa tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc
     1321 atctccgtca tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc
     1381 atccgagtta gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc
     1441 ttttgccaca atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac
     1501 aacgaatatg aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag
     1561 ttctcttttt tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt
     1621 acagtttgga tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata
     1681 tatacgtcca ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa
     1741 acgtcacgtg ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct
     1801 ctttttgtta gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata
     1861 gtcaaatcgc gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg
     1921 attcgacgca acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg
     1981 atgtagtcat ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca
     2041 gtcatcataa tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg
     2101 ttcaattctg ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca
     2161 tctaaaccag ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct
     2221 agctcgacaa atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat
     2281 tattttatcc tttctattct actggctttt taaaaactct atcattatac cgataatatg
     2341 cttaaaaatc tatagtcatt ttatcatgga tagcaatttt aaaaaagagc tgaagtctac
     2401 tcataacaat cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg
     2461 aaagactatt attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc
     2521 tcataggtat gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt
     2581 tctgctctgt atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca
     2641 gcttggacag ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca
     2701 acgaagtaat catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta
     2761 attttttgca agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca
     2821 ccaaatggca agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg
     2881 gcataactca tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt
     2941 ttgtctcccg tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt
     3001 tcttctttat aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc
     3061 acacctaagt caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca
     3121 tcaaggatca catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg
     3181 tcagcagtga tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata
     3241 tcagccattt cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca
     3301 atcagttgca tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct
     3361 ccatttgggc caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc
     3421 tctaagattg gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt
     3481 ccttttaaag ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag
     3541 ttggctaact cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc
     3601 aaaatatcgc ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt
     3661 cctgctttga ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg
     3721 attgtatatg ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta
     3781 gcccctgtac gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga
     3841 tttttaccca taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct
     3901 cctacatacg ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa
     3961 actaaagaac aacatatt
//
LOCUS       DAZUMI010000178         3899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000178 DAZUMI010000000
VERSION     DAZUMI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.186"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..605)
                     /locus_tag="ACG81C_002796"
     CDS             complement(63..605)
                     /locus_tag="ACG81C_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5065897.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            740..1129
                     /locus_tag="ACG81C_002797"
     CDS             740..1129
                     /locus_tag="ACG81C_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065898.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1133..1897
                     /locus_tag="ACG81C_002798"
     CDS             1133..1897
                     /locus_tag="ACG81C_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5065899.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2026..2973
                     /locus_tag="ACG81C_002799"
     CDS             2026..2973
                     /locus_tag="ACG81C_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065900.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3103..3843
                     /locus_tag="ACG81C_002800"
     CDS             3103..3843
                     /locus_tag="ACG81C_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065901.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1292 a          667 c          799 g         1141 t
ORIGIN      
        1 aataaaaact gtctaacatt taaaatttaa tttagatatt agacagtttt taagtttgtg
       61 agttattttc cttgtcgttt tctcactttt ttagtccaga aaccacctga aatatatttt
      121 ggctcataag aaatgataaa agcacgctct tcgatttctt tgatcaggcg atacagttca
      181 cgttcgcttt ttctaggaga aagtatttcg agaactagcc gctcaccttc cagccccatt
      241 gcagaagtct gtgtcacacc ataaccgtgt tctctcagta tacccggcaa acttttatct
      301 tctgcgctat ttgctggcaa aatcgttgtg accatgatgt atcctaatgc caatttgtct
      361 tcaatcaaaa ttccgacact gatacctact gcgtaaccta acgcatagac aaataaattc
      421 agtggattat ctaaacgatc taaaacaagg gataagccga cgatataaat cgtgatctcg
      481 accatactga caagtggagc aatcagtcga tagcctttca ttgtcaacat gaaacgtaac
      541 gtgttcagag tgatataagc aaaattaatg aggaagatca tccccaacat ttttggatca
      601 atcatttgta aaactccttt catttatata acaacaatat caaaaaaaat cgtaagtgaa
      661 gaaaaattga aactttatac atacaaaaaa atgataaaat gattataata gaattataaa
      721 gataggttag gtgattaaaa tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct
      781 aagtctcctt ctcagtatat acttaggtgt ctattcgatt caacagccaa ttgaggtaga
      841 gaacattgtt tttcagatgt cgctgacaga tggtttaatt ttaagcgttt ttttcttttt
      901 gatactgttt attggtaata ttttcggtag cgttgccagt tttttgaatt tttctattta
      961 tccactcctt tcgctagcat taggagtggt gggactttgt tctctgtgct tatttcccga
     1021 gccattcagt cctgcattta tattatttgg tacactgaac ctgttccaga caactgttgg
     1081 agcatggttg ctatggcgat caggaaattt aatgaaaata ggtgagtaac aattgaatcc
     1141 aattatattg aaacgttctg tccatcatat tccagtgtta gaagttgttc tgaaagaatt
     1201 aagagaggaa cgtattcccg ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt
     1261 agttttgacg caaggaagaa aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc
     1321 gatgaatcat ggggagcgca aacagccggt ttcttctatt ccatcctata cattttggga
     1381 tagtatctac ggaaatctgt ttgaatttga cacacttatc aagcatttag aaaaaagagg
     1441 tcttctacat gatcagcttg cagttggcgg ggtttcgatg gggggaatga caacatgcgc
     1501 cttgttggct aaacatcctc atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc
     1561 tttggaatat ggaaaagaaa tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa
     1621 agactatttt gatttgattt catgggttaa gagctatgat ttgtcattgc agccggaaaa
     1681 gttagctgga cgtcctctgt tcttttggca tggacaagct gacgaaaaaa tcccttatat
     1741 acaaacggaa cgatttgtag aaaaaaatcg gcataagtct tacggaaaag aaattgtttt
     1801 tcgttcctca aaaacacagc gacatatggt acaaatacca ctaatggaag aagcttccac
     1861 ttttttagca aaaaatttga taaaaaagta taaataattg aaacggaaaa tcactcatga
     1921 cgatagagga ttttccgttt tttattttgg gctataataa actctgttag gaaaatttgg
     1981 tataattgat agtaagaatg acaacatgtt agaaaggaag agagcatgcc cactttagca
     2041 gatgttgcaa aacgtgccaa tgtttcaaaa atgacggtct ctcgagtcat caatcatccg
     2101 gaacaggtga ctgacgaatt gaaagagtta gttttctcag caatggctga actggattac
     2161 cgtccaaata ttgctgcaaa agcacttgtc acgaatcggt cgcagatcat caaattattt
     2221 atactggaag aaatcgacac aactgagccc tattacatga atttgctgat gggaatcgct
     2281 aaacggatcg gtaaagcgca ttattctctc caattagtta caaatgatgc cttcgacgtc
     2341 ggttcttgtg atggatacat catcacaggt gtcagaaaaa aagacttcga ttggatcgac
     2401 cgtttggaaa aaccagttgt cttgtttggg gaaaattctg aaggatatga ttatgtagac
     2461 agcgataatg aatatggtac agcaaaagca acgcaatatg ccttagaaag agattatcaa
     2521 acgattattt atatcggtat cgaagtggac gagccttttg aaatttcaag ggaaactggt
     2581 tatttatctg tgatgaagga agttgatctt cctagtaaaa tcttgcggtt tccaaatcat
     2641 tcgacagaag tagaaagata tatgacagaa aactgggcag attttacaga taaaacttgt
     2701 tttgtatgta gttcagatcg gctagcacta ggagcagtaa gagggattat aaatgctgga
     2761 ggacacttgc ccgaagaatt tggtgtgatt ggatttgatg gggtattttt agaccaaatc
     2821 agttcaccta aattgacaac gatcaagcaa gatattatac gtatgggtga agtatgtgga
     2881 gaaatgttgt taaaaaaaat agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt
     2941 cctgaactag ttatcagaga gacaacgaat taagtcttat tactatatat atatttatga
     3001 aattcatcgc tattttctta aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg
     3061 ctattattga gaaaagttcc atttttgaga aggatggttt gaatgaaaga aatcagacga
     3121 aaacatttcc gttttcctgc gtacgatgat gagttaggcg taaaattaac caacgacaag
     3181 aagcgagtat tatttgacgg acaagaagat cttttgactg aacagaatca gtccccgttt
     3241 gaacagatga aagaatttca tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt
     3301 cgtgcaacaa atggattgaa gtcagctccg cgtaaggaaa atttggaaaa gcataaagga
     3361 aaattaccgg attataccgg ccattaccaa tcagaaatca catcgacagg acgaaaaaaa
     3421 ctttttggtg attcctctcg ttctgtcaga caaagtgaaa caaggacaga gtccaagcat
     3481 actacacgaa acgcatctgt tgacacgaag gtgcgggagc gttcttattt tgttcctaaa
     3541 tatgtacccg catcgattat cccagaccct agagaaagca aaatttctga ggatcattta
     3601 atcgaatcga tgaaaaaaac ggatgactct tatctgttgt tcgatacaga accgacgccg
     3661 tatcaagaaa aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta
     3721 gaggaaacaa agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc
     3781 tcattacaag gattgatcga tgatcaagga aactctttgg gagaaaatag ctattttaaa
     3841 taagtgatat acaaatttat attcggctac agttggaaag gaaacgctgc ggcgtttcc
//
LOCUS       DAZUMI010000179         3888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000179 DAZUMI010000000
VERSION     DAZUMI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.187"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            429..1028
                     /locus_tag="ACG81C_002801"
     CDS             429..1028
                     /locus_tag="ACG81C_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5065902.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1077..1793
                     /locus_tag="ACG81C_002802"
     CDS             1077..1793
                     /locus_tag="ACG81C_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFS5065903.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1807..2586
                     /locus_tag="ACG81C_002803"
     CDS             1807..2586
                     /locus_tag="ACG81C_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5065904.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2669..3025
                     /locus_tag="ACG81C_002804"
     CDS             2669..3025
                     /locus_tag="ACG81C_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5065905.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            3015..3281
                     /locus_tag="ACG81C_002805"
     CDS             3015..3281
                     /locus_tag="ACG81C_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5065906.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            3440..3718
                     /locus_tag="ACG81C_002806"
     CDS             3440..3718
                     /locus_tag="ACG81C_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065907.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(3715..>3888)
                     /locus_tag="ACG81C_002807"
     CDS             complement(3715..>3888)
                     /locus_tag="ACG81C_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5065908.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         1443 a          555 c          747 g         1143 t
ORIGIN      
        1 aaacaatgat gaaatcattt agttcttata aattagacaa gagaaaataa atattccata
       61 tctactttat tggatccata acatgactaa attctgaata ctttttaaag cttatcattt
      121 ggtaaaactg aatataagtt aattatatag actgaaaatt ttgaattttc agaatagaat
      181 aacttaaaag tactgacaaa ttttttaaag gggcaatatt aatgttctga gataatcaaa
      241 tcttaccgaa aatatcaaaa gtccaatgtg acacaattgg attttgtcac attcgttaaa
      301 atcgctccat ccttagaggc tctaatagga aatcactttt cttttagcgc cttttttcta
      361 tagcttgttt aaatgtgact tattatctgt tataatcgta ttaagtaaca tttgagaggt
      421 gtctaaaaat gaaaagcgtg gaaccgattc gcgacaaaaa aaagatcgat gccatgaagg
      481 ccattttggc ttccggaaaa tatgggcaac gaaatttggt gcttttttcg atcgggatca
      541 atacagccta tcgaatctcc gatttaagac agttaaaatt aagggatgtg ctagaaattt
      601 cccgtgggcg ggtgatcgtc aaagaacgtc tggccatgaa ggaacaaaaa acggccaaac
      661 acaattcggt cttcatttcg aacaaattgc gaaaagtgat tcttgattat gttcaatccg
      721 aatttcctga gcagttgcaa gcacaagact tcagcaagta tttgtttcca agtcgaaaag
      781 gagcagatac gcctttgacc cgacaaagcc tttggcggat cattcatgaa gcaggaactg
      841 cagttgggtt aaaagaaatt ggtccccatt cgatgcgtaa gacctttggc tattttttat
      901 acaagcaagg aaccaaaaca gagatcattc agtcgctgct aaaccattct tcacaacggg
      961 agaccttacg ctatattgga atcacccagg aagacaaaga tactgcagta aagagtttgg
     1021 atttatgaca gaataatgcc ctatataata gaaggaaaaa ttcaagaggt gagaaaatga
     1081 acgaagaaaa gcagcctaac tttcctgata aatatcactt atctcgcaaa gagtctgtgt
     1141 atttattgaa gaaaaacatt gtagaactgg tgtataatgc ggggaaattt gaagggttaa
     1201 atacaacgtt gttacaaaca gaagggatta ttaagtataa tcgggccaac aacgtggctg
     1261 tagatgatgt cctgaccgtc gttaacttaa aaagaggctt tgaaatgctt ttaaatgacg
     1321 tacaggagcc cctgttagag acaagtaagc ggattaatcg gatcgttgca gcagaggatg
     1381 cgctttttcc cggtgaaata cgaaccggtg gggtagaatt atcgacgatt caaggaagat
     1441 atgtgccacc aatgttaact gaagacgagg taaacaatca atatggtgag ataatgaatc
     1501 aagagatatc agatacggaa aaagccctgc gactgtttct attcatttcg aaaaatcaaa
     1561 tattctggaa tggtaatgtg ggtacagcct tgttgattgc gaataagatt atgtttagca
     1621 acggtttagg actattatca atacccgaaa atgtatttat gaaatttaac gaacttttat
     1681 caaagtattg caacagtaat ctattagtca atgagtcaaa tattatatca tttttatatg
     1741 aacagtgtat ctttggtatt aattatttga aaataatcca acatacaatt taagaggtat
     1801 aagtaaatga tgactataga cgaagcaaaa caattagcaa cttatttttc agttggttca
     1861 gcgggatata ttttagactt tactaatgta acgtggaaca tatttacaaa gaaaatcgta
     1921 gggattgaaa tacaaaatca ttatgggggc tcaaagggag tatcctttca aaagtttctg
     1981 cttgacgatc aatttgatga ttcgttaaag aatcggttaa tccaatcttt aaaaggtatt
     2041 atggatcgag ataaaaaata tgaatctact gaaatgaatc ccatagttca gttgcttttt
     2101 gaggaagtat ttaaagaaga tacttcaact gcattaagaa aaatgaaatt ttcaagcgag
     2161 attgatttac tttatattcg gcagctaccc attaggattc aaacagatat taaaaacaag
     2221 gattttgact ctgttttgac aaaagcaaat actttaatag atgaagttct aaaatttatt
     2281 attgaaaata ccgaaggacc gagtgttaat attaatttat ttaagtcaaa agaattaaga
     2341 aaagaagtat ttggccgcct caatattaaa actgaaaaag atatggataa tagaatcaaa
     2401 gcccttgttg gagcactcaa tacattatca gataaaatac tcgaaatgag aaatagtcaa
     2461 ggagatgctc atgcacatgg ctcaaataga atagtaatta aggaagaaga agcaattctt
     2521 gttgcaaatt cttctatggt tttgtgcgag tatgtgttta ctaagtataa aggattaaat
     2581 agataataaa atgcatattt tatagtatac aattcgaatt tttatgttat atactataat
     2641 ataaaatcaa tatatgatat aattgggtat ggaaaaaata tcttttgaaa catttaaaag
     2701 acccaatggt catgatgaat ttttagaatg gttggaaaca ttaccaaaaa aagatagtgc
     2761 gaagctttta cgtacaatag aagaaacaga aaagaatggg atgctagtag ctcaacggtt
     2821 gaaatgggtg aaaaaattag acactaattt gtacgaatta agaagtaagg taggatcaaa
     2881 catccaaaga gccatctatt tccatgtgga caatggaagg tatgtcatta ctcacggatt
     2941 tactaaaaaa acggagaaaa caccaataac tgaactcaag catgcaaaag aacttagaga
     3001 ggagtggttc acaaatgatg attaaaaaat tgattgaaga aaagaaaaaa gataaagatt
     3061 ttgcagaagc atatgaccgt gaaggaagaa aactcgaaac tgcggttgct ctttatcatg
     3121 ctcgggagga agcagggcta actcaagcgg agcttgcaca aaaagcacat acaactcaat
     3181 ctactattgc tcgaattgag agaggggata acgtatcttt cgataaactt gatgatatcg
     3241 ccagggcatt aggtaaaaag ctggtagtag aatttgctta aaagcgagtt gaaaatatat
     3301 ctataatagg ctcctaacta atcaattagt ttagtgggcc tttttttact agatagataa
     3361 aggttgtact atagtgtaaa agacaataaa ttttatacta aaattacttt aaagagaact
     3421 agagagaaag gtacaataaa tgaaaaccat aaatcttata aaagtattta tagcatcacc
     3481 aggtgatata gcagcaaaaa ggaatgaagt agaagaaacc atatatagat ggaatataga
     3541 aaatacagat aatacaaaag tgattttgat gcctatacga tgggaaacta atgcgattcc
     3601 tacttatgta aataatagct ctgggcaaag tgttattaat aaacaaattg tagaaaatag
     3661 ggttctgctg caaagtttta aaaataaaat cagtttttgt attatttctt cattttaagc
     3721 tggaattccc aataatatcc tgatttcagt gcagactgaa aagccgaaga gagtgccttc
     3781 ttttcggctt ttcttgtaga ttcctcgaat ggtttccatc cctttaattg tggtggaagc
     3841 tgtacgtaga ctttgataca atttatgtcg acgttttatc gatcgatg
//
LOCUS       DAZUMI010000180         3888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000180 DAZUMI010000000
VERSION     DAZUMI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.188"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2625)
                     /gene="brxC"
                     /locus_tag="ACG81C_002808"
     CDS             complement(<1..2625)
                     /gene="brxC"
                     /locus_tag="ACG81C_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS5065909.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPS"
     gene            complement(2650..3225)
                     /locus_tag="ACG81C_002809"
     CDS             complement(2650..3225)
                     /locus_tag="ACG81C_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFS5065910.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3225..3824)
                     /locus_tag="ACG81C_002810"
     CDS             complement(3225..3824)
                     /locus_tag="ACG81C_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFS5065911.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1164 a          749 c          531 g         1444 t
ORIGIN      
        1 gcttggttca atttttatct tttcactttc tcgtcgtttt tgaataatca ttacaatact
       61 atttggatca tccatccgtg aaatttgtac attgttataa agtaaatcaa ttttaccaga
      121 catgaaaagt ttagcgatgc accattccac atcaagttct gtgaatccat acggttgttt
      181 tgtttcaaaa cgatcacgaa ttgttttcat cgtaatcgtt aggtgatttc tcttttgatc
      241 attgataaaa ttgaaaattt cctgaactgc ttgctcattt gatggatctt caataatttc
      301 atttttgctt tctttcacta aatataagat atcttctttt tctttaggcg catcaatttg
      361 gtttaattta tggtatactt cttcagccat tttctgaagt gctgattgga attttgcttt
      421 aaagtcggag cctgtcagtt gaatttctgc accgttatag aaaaattttg cagatttcaa
      481 cagttcattt aattgatcgt tgatacttgc acttagttcc tgtaattcac cagatttttc
      541 cgaacgaatc gcgtcaaagt tctttactac actcgaataa gtcgttgtta aataaagatt
      601 cattctagca tacattgcta tatcatcttt aaattcttta tctgatggca attctacgaa
      661 taataagtta cgggaactct taagcgcaag ttctgcctct tgacctgcta aatcagagta
      721 tggtgaaata aaattaactg tcatttcagc atttccagct tgcttgtatg gacgttgatc
      781 taacacttgt gcataagtaa acgtataccg gtttttaaat ttatttccac ctgacatgcc
      841 taatttttca taggcaaaac tatttcggtc tagaacgccg tcaaaaattc gtttagatag
      901 ttctacgatc aaatcactat ctgtaatatt ttgattttta atctcacgag taatttcctg
      961 ttcttcatca gtttggaaaa tataactatc taagttcttc cgcacaaagt tttccttaat
     1021 taaaaggtcc aatgcttctt ctactcgttt tttagtttcc aggcggtctt cgttgatatt
     1081 tcttatcatc aagctcgtaa tattttcaac atttggtttg atggcttgat caacatattt
     1141 aatcatgaat aaaattcgta agacatttac gttaaagtta tctatatcag ttggtctttg
     1201 aatatgagaa ttatttgtag cttgtgtaat cactcggcta attgctgtat caatccaacg
     1261 ttctaacgag taataaaact gatctaaaga aaccaaactg gttgtatcat tttccttatt
     1321 tcgttcagcg gcttctttaa acattgctag taatgaacgt tcaccagacg ataagtttga
     1381 tccttgatag ctgtgattac gaatctcggt tagaatttgc cccaataaat tgaattgata
     1441 gggaataaat ggataaactt ctgcgtaatt ttcagcatct tggaagagct ttaattcagg
     1501 actatcaaca aagagtaatt tatttttcaa ttctgtttga acagtttcaa attgtaaggc
     1561 taaagaatca ttggcaaact cttttttctt caaaatacgt tctttaatta cttcatcggc
     1621 atttgctgaa gataaactca cagttgtggc aaaacgatcc ttgatcttag aaaaatcatt
     1681 atcagaattg ttatcaaagt ttgttacgtg atcaatcgct tcctgacttg tcactaatac
     1741 ccaagcctta ccttcacaaa ttcgacccaa atcttccaca acggtttgta actgcaaagt
     1801 caactcacga ttaccgccga tatattgtcc gacctcatcg actagaaagg caatatgatg
     1861 gtctgcttct ttggaatcaa gatagctttt aactagattt gcaaagtcat caatgcttat
     1921 taaataattt cctgatgctt tttctaaaaa attatcaata gcggcttcac tcataaaatc
     1981 gattttaagt gccgcttctt tgatatcatc ttggatccaa tcaaactcat ggcgctcttc
     2041 ttgccatttt gccccattaa tctcttcaaa agcagctttg aatgcatcaa acttttcttc
     2101 tttaattaac cgacgttcaa aatctgctag atgtggaatc gtgcttaatc cttgcatttc
     2161 gttaaatacc tttaaaaaaa cattcacgat tccgtttttg tcttttttac cacctgcatc
     2221 actttttgaa tcaatattaa acagaataac atctgtattt atttgtgagc accgttccat
     2281 cgttgccaaa gtcagtggat ctttgatttt cccatcttct ttgaagtaat caatggcttt
     2341 cttgtcattt atttctcgat ttgcgagcaa gtaagaaata atttttaaga aatgagattt
     2401 acctgaacca aagaacccct gaatccaaac acccatttta tctgttggtg tattgatact
     2461 ccgttggtag gcatcaaaaa ttgttttaaa gtgtttttgt agttctttcg taaccacgta
     2521 ttcttctaat tcttgttgaa cattttcatc ctttgcttga ccgaccttaa taactccttg
     2581 aatatttcgg tcaattggtt ttgcaaaaat atctttaatt agcatttaat ctcctacctt
     2641 ccatcccaac taaattctaa atgctcgata ataattatca tctttattgg tattaagaat
     2701 tgtcaaactt aattcatcat attcccctgg atacatcatc acaactggta cgcgatcaaa
     2761 tacctgatgc attttattta aaatagagtg cgctctaatt aatggatata attcgccaat
     2821 tcctgtcagg aacacaactg atttttctgg tgtatgggca tccacatatt caaaaaatac
     2881 attttcttcc tcaggtaaat ccattttcaa taaacgacca atgctttcca ctaatttttg
     2941 taaaccacgt cgttcttcca tcttaatcgc tttttcagtg aaaccgcgtt tttctagata
     3001 ctctaacata atcacataga gattataaac tttcacttca taattatcac caacagatgt
     3061 tccagatata tcattaatat aatctcttac aattaactcg tctttcggat cgtagtcaaa
     3121 gatataatag cctacttcat tacctaaacc acgattcaat aaaaaatctt ctttttgaat
     3181 tacttcattc aacttttcca aatgctcttg aatcgataat gacattataa aattcctccc
     3241 agtgctagta caatatcttt acgattcttg tcaactaacc attgaatcaa tcgtttatct
     3301 aataatatcg gagtcattcg tccatattct ttttcccctc tgactaatcc tgtttcaatt
     3361 agattcgttt gataagcggc aataagccgt ttaatcgtat aatctttcca ttttgaaata
     3421 tcttctgaac ttctccgctt ctctgtaaaa aaagaacggt aatcaactgg tgttaattgc
     3481 aaatttcctt tacgtagatt ttcagcatat acctcagtca taaattcatt aaaaagatca
     3541 tcaatcataa ggatggaaat caaattcact aacttttgat tatctatatt taactgattg
     3601 aaaagttgca atccagcttc atccagttga ctaactctcc ttttcacgtt ctgatagatt
     3661 tctttttgac ggttactgct cactgcaaaa aaaatatttt catgttcatt tttctcttta
     3721 atttctatat ctgtatatcc ttctagtcgt aattgaatat ataacttgaa ctctgaaaac
     3781 caaaagcgtt tagaaactag accagcagaa tattttttat tcaatagatt gcctcctaat
     3841 catctataat tcaataagta aacaagtgtt ttttgctcat cgttagca
//
LOCUS       DAZUMI010000181         3874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000181 DAZUMI010000000
VERSION     DAZUMI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.189"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACG81C_002811"
     CDS             complement(1..1155)
                     /locus_tag="ACG81C_002811"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5065912.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="ACG81C_002812"
     CDS             1340..3781
                     /locus_tag="ACG81C_002812"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5065913.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1234 a          725 c          847 g         1068 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt tggctcttcg tcaaattgtg ttgg
//
LOCUS       DAZUMI010000182         3821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000182 DAZUMI010000000
VERSION     DAZUMI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.190"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..428
                     /locus_tag="ACG81C_002813"
     CDS             120..428
                     /locus_tag="ACG81C_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5065914.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            455..754
                     /locus_tag="ACG81C_002814"
     CDS             455..754
                     /locus_tag="ACG81C_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS5065915.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            928..1473
                     /locus_tag="ACG81C_002815"
     CDS             928..1473
                     /locus_tag="ACG81C_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS5065916.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            1629..1925
                     /locus_tag="ACG81C_002816"
     CDS             1629..1925
                     /locus_tag="ACG81C_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065917.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            1918..2808
                     /locus_tag="ACG81C_002817"
     CDS             1918..2808
                     /locus_tag="ACG81C_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFS5065918.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            2890..>3821
                     /locus_tag="ACG81C_002818"
     CDS             2890..>3821
                     /locus_tag="ACG81C_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5065919.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGG"
BASE COUNT         1419 a          557 c          755 g         1090 t
ORIGIN      
        1 attgaaataa ataatatagc aatgaaatag agataaattc aaatatatat tatatccatg
       61 aagtccaatc ttctttatag aagattgggc tattttttac gacaaaggag ggaaaagaaa
      121 tgtttcaaaa taaaggacaa cgttatgtca caaagggagt aatggacagt ttactggtgg
      181 aattacaaac tttatgttgg aatttaatcg atcaaaacgt tcaaaagaag cttcctttag
      241 actatcttca aatctttgaa ttttcgatag aaaaaggaaa tcaaaagttg gttcatcgtc
      301 aagaagaacc ggaagcaaga aaagagtatc tgatttcacc taagcttcgt ttggaatcag
      361 tgtctcaaaa aatatgggtc attgattcgg gtgagtatca aacgatgtta ttaccagaag
      421 aatactgata tctaaaagaa aggagcgaaa tgacttgtta gaaacggcta tcgaagtact
      481 ggaaaagtgt gcacagctag tcactactcc tgaagagtgg ggctatgaat ccgttacgat
      541 ggaaaaagag gaaattgaaa tgggtacgct tcctaaaaat gttcatctac cacgtttagt
      601 gatgacacat ctttatattt attgcgcacc agaagatgga aaagactatg tagtttattt
      661 cattacagat attaccagcc agcgagaatt tgtttgtggg ttactggtag aaggacgatt
      721 ggtttggagt cagattggag gaacgaatga ataagatcga ttatttgaga atgattgcga
      781 aatgtttaga agatatcctt accgcgattt atcgaattct gaagaaaaaa ggagaaagaa
      841 tatgaaatat tttcaactag tattgacact tataacggca atggtaattg aattgcttga
      901 acaaaaatta gcataggaga aaaagagatg attgaaaaag aattagtgac cacacaaaca
      961 gaagtttggc gagatcctca tcatacccat cgctatgttt tcaaacgaca atgggcaatc
     1021 aaaggaaaag aagagaaaga aaaattagca gtagtcatta ccattcgtcc aacagataca
     1081 gaaccattta cgaatgattt gagtatgtta ttaatcgaaa agaatatccg acaattggga
     1141 tttactggtt ttattgccgt gaatcttttt tcgtatacaa aagcgaaaag tcctcgggta
     1201 ttttctcgtg gcaatgatga acaatctttg gaaatattaa cgaccatttt tacagaaaag
     1261 aaaatcaaca ctattatttt tgcttgtggt tcgattacgg caacgagtca agtggcttat
     1321 gaacaagcaa agaccattta cgatcaattg agtgcgaaac aaaagaagat gaccaaagta
     1381 ttgataaatg cagaaggcaa gttatctcac ccattaagta ttcatgttcg taatgaatgg
     1441 caattgtcgg atcattcttt gttatttcaa taaacgaaaa taagtattca atgtatatgt
     1501 ttctttttct gttattttac ttgttctttt gcagactaaa caacttgtaa aacctcttgt
     1561 tatatgcatt atcaatgaag gaatcaatat gatattttta aaatgtcagg ggaaatggag
     1621 gtgagcaagt gaaagtagat ggggaatgtt tgacgagttc gtataaaagt atctatgaat
     1681 tttttgagga aaaaggacta gacacggatt tactttatca gttttatatc gcttttcgtg
     1741 ggcaacagtt gtcgtttcca atgaaaatgt atgaccgaga attagtcgct caaaaaattg
     1801 aaacaatgat acaagaaaac cattccattg attctaggaa attaacggat cggtatggtt
     1861 tttcaacgag atggattcaa agtattatca gacaacaaca aaaggagcaa catgtacatg
     1921 gctaatgaag aaacagtagg tttgagaatc atagaagaag cattcaaatt accaatggtc
     1981 aaagttgatc gtcgagcatt tttgattcaa acatttcaag ggaatgtttc tgatattgaa
     2041 ggactcatag aaaagggtcc acaagcattt ttatccaaag aaatgctaga tcgtgaagca
     2101 caaaaatgta ttcaatcgat taccttgaaa tcaagtgcgg tatcgttagc aactggactg
     2161 ccaggaggta tcgctatggc agcaactatt ccagcggatc tccttcagtt ctttggttat
     2221 tcattacgat tagcacagga aatttcgtat atttatggat ttaaggattt aatgggagag
     2281 actggagagt tcagtgaaga agcaaagaat acgctgatat tgtatttggg aattatgctt
     2341 ggagtaactt ctgcaggttc tgccgtacga gcgatgtctg gaaagctgtc tacacaagca
     2401 ttgaaaaaga ttcctcaaaa agcattgaca caaacgctgt attatccagt aatcaaacga
     2461 gtactaggta tttttggtac aaagttaact aaaaatacct ttgcgaaagg tatttctaaa
     2521 gcagtaccag ttgtgggagg cgttgtttca ggaggcatca attacttgtc tatgaaacca
     2581 atggcaacga agcttcaaac agagttaggg aaaaatgtag cgtatacaga aaaaaatctc
     2641 caacaagatt tagatatttt agaaggtgaa tttgaagaag tatcttctga tatcgtgtct
     2701 tctgatacag ttctttctca attagaaaga ctttctcact tgttggaaac gaatatgatt
     2761 acagaagaag agtttcaaca attgaagcaa gagctgttaa agaaataatt atttagaaaa
     2821 agcaaccact tatacgggtg gttctttttc ttgaatgaat tcaaatttat tattaggaga
     2881 agaaaaagga tgaaagcaaa acgattattc ctattaggca cgattctttt acttggagga
     2941 tgttctactg tctctcaaaa tgaacaaaag aaacaagtgg attcaacaac ttatgaaact
     3001 gaaacaacaa gaaaggaatc ttctacaaaa gaaatagaaa caactactca agaaactaaa
     3061 acaacagagt cttctagtaa ggcaacgagt tcagatacga gtgattttca aataaaacaa
     3121 gaattatggg atacaaataa agcatctcaa ttagcaactt ttgtcccaca atggggaaag
     3181 acattaggtc aagaatataa aagctacaat cctcagaaca atgtaagtct gtatggtacg
     3241 cctttaccaa gtgcagttat tgatggaaat tggaaaatgg caatcaatga agcaccagtt
     3301 actgtacaat ggtcagaaga tggaactgga aatgcaggat ttaatttagt agcagtttat
     3361 tctgatgtag aaactggaga atatttaggg cagcatgttt actttttcgg ttttcaaaat
     3421 ggacaaccta aagtttattt aacgcaacaa aatcaaggta atgaaaataa ttatctgtac
     3481 tttaacgaaa ctcaaaatca acagctaaaa caaggattta cagatatcat taatggacaa
     3541 actccccaaa caccagtagt agaagaatct actcaacaag caacaactac aacgacacaa
     3601 tctacaagta gttcaggaat ttcaaattgg gaagctgcta aagaaactgt actaaataat
     3661 aaagcgttat ggatttatga gtctccagat aaaaatgaag caaatactaa tattattgaa
     3721 tccacccaat atagaaaaga tttaatgcaa gatgaaaatg gagaatttta tccagttgat
     3781 atttctactg atgatattgg aaatacggta atgaatggag g
//
LOCUS       DAZUMI010000183         3777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000183 DAZUMI010000000
VERSION     DAZUMI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.191"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..728
                     /locus_tag="ACG81C_002819"
     CDS             126..728
                     /locus_tag="ACG81C_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFS5065920.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            1083..1319
                     /locus_tag="ACG81C_002820"
     CDS             1083..1319
                     /locus_tag="ACG81C_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFS5065921.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            1316..1729
                     /locus_tag="ACG81C_002821"
     CDS             1316..1729
                     /locus_tag="ACG81C_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5065922.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            2272..3198
                     /locus_tag="ACG81C_002822"
     CDS             2272..3198
                     /locus_tag="ACG81C_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFS5065923.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(3195..>3777)
                     /locus_tag="ACG81C_002823"
     CDS             complement(3195..>3777)
                     /locus_tag="ACG81C_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5065924.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1380 a          503 c          675 g         1219 t
ORIGIN      
        1 acagatattt cattttattt atgtaaaaca acacgaataa ttattgcttc gtaaagttat
       61 atgagtataa gttaatttac tggaggtagt gcaatgcttt tttctgaaag atttggtttt
      121 aaaaaatgga ggatatcata ctgagagata gattgacaga ccctcttaaa aatagtttag
      181 caaatgtgat ttatgatata ttacgagagg ctgataaagt ttatagtaat tataatggaa
      241 aagtctcatg ggtaatatat tcttttttaa ttaaagagtt ttttaaagaa cagataaata
      301 atagctatga gttgggttat cattttactg tttattatga agatattatc aagaggttag
      361 aagacttgga gtggtatgct tattatgatt tattagaggt ttttgcaagt gctaaaatct
      421 tggataaaaa gaaaagagag gaagtaaata aaattctgga gtcggaaaat tctggttaca
      481 gattaagcga aagcaatgaa tttattccaa tcacagatga tatttctatt aagtctataa
      541 atgctgtatc tgaatcacca tttaattacg caaaaaatca tatccataaa tctcttacat
      601 atataagcga gaaggaaaat caagattata atactgtagt gagagaagca attagtgctg
      661 tcgaaagttg tcttatcgaa ttttcaggat taccagctaa taaaaaaata ctttgggaac
      721 tgctataaaa gcaattaaag acaaatcgaa taacaatgtc gatcttacat ttttaaaacc
      781 atttgagaca atgtatggtt tggcgagtaa taatggaata cgacatgcag aaaatgaaaa
      841 aacaattgtc tcagatttac ctgatgcagt tttggtgtta acaacatgtt cagccgtaat
      901 taattatttg ggagtaaaaa tagcatatga ctccaataaa tcatgaaaag ttaaaattca
      961 tccaagagct acattataac tattaatttc caactcttta aaagtttagt aattgaagga
     1021 agtaataagg gtgattgaac aatacggata tacggtgtat acttatcgta ggagttgatg
     1081 atatgaatat taaaaaacgc aaaatcagag aagtaggtaa ttcaatagtt gtaactttgt
     1141 caaaagagag tttattgcaa aaaggtttga aacctggaga tactattttt attgatgaag
     1201 ataaaatgat ggaagctatt gtaaaagagg aaagcaagat agatcttgaa attgatatgt
     1261 atgttaacca agctttatct gagtatgatg cagctttcaa ggaattagtt gaaaaatgat
     1321 ctatctaagt gaaaaagata ttgtaaaatt gaatgttatt caaataaaga aatattcacc
     1381 gcgagaaaca atcggagtca ttgacaaaaa tgcgctgagt atggctgtaa atcaacctaa
     1441 acagcatatt tttggaaaag atctgtatcc tgataaattt agcaaagcag ctgttctgct
     1501 aataaatctg atcaaaaagc atccatttca taatgctaac aaacgtacag catttttagc
     1561 cgcgtatgtt tttctcaagc taaatggcta ttcactaaaa atgaaaaatc aggaagtagt
     1621 agaatttgta gttgaaattg ctacgtatca gggtgatttt gatgacttaa aagaggcaac
     1681 aacagatata ctaaaaaaga attcaacaaa gaccgattca aaaaagtaga agcaaactag
     1741 ggattcagtt aaatctgaag atttagtttg ttctttattt tagtttacat aagatacatt
     1801 atgcgaagtt cattatttta aaagcttaaa taataaactt ttttataata ccaatttatt
     1861 tatgtaaacc aatgacctta atcgcttaag caagcatgtt atgtaatact ctactctctc
     1921 caaaactaat gactatctat tttcaaagaa gctagaatga ttatctgatc aaatgataat
     1981 ttatatttaa ttcctggtct actgctcttc ttttaatcaa ttaatgtcta acgtactgta
     2041 cacgataaag taggaagact tattttcaac ttcggattca tttttttaat tcggtgaaaa
     2101 tcactaaatt aagagtttat ccaagttcat tttagctgag aatacgcttg aaaataattt
     2161 tgatgaattt tcctgaatta aaatttatta acttgttgag tgatataatt gtaatgtatt
     2221 accaaatagt tagcacttag attagtgatg gaaaggaata gtgagaacaa gatgactggt
     2281 agaaatccca aagtatttat atcatattca tgggagaata aggaaaataa agaatgggct
     2341 agagaactag cagacaaatt acttgaaaac ggtattgatg catatataga tcagtatgat
     2401 ttaagtttag gtgatagatt acctcagttt atggagcagc aaatatccga ttcagattat
     2461 gttctaatta tatgtacacc agagtataaa aaaaaggcaa ataatcgtac aagtggtgtt
     2521 gggtatgaag gtcatattat ctcagcagaa ttgctttctg gaaaaaatga acgtaaattt
     2581 atcccaataa ttaaagaagg acgtgcaagc gaagtacttc ctacttttct tgctggaaag
     2641 cttggaattg atttatcaga tagccagcat tatgatgaaa acttagatga tttaattaca
     2701 actatatttg gtgttaagag aaaaccagca ttaggtgata tacctaggca tattaaaggc
     2761 agtcctttac tacctataga acataatgaa aataaagata ttgaaatctt aggaattatc
     2821 actgataaag taactgtacc taaaatggat ggctcaaggg gttctgcatt atataaaatt
     2881 ccatttagat taaatagagt accaagtcag ctttggaaag agttgtttgt tagaaattgg
     2941 aattcaccac cacgatatac aacaatgcat agaccaggaa ttgcttctgt tgtagacgat
     3001 gaaattttac ttaatggaac aactattgag gaagtccggg aatatcatcg ggaaacttta
     3061 aaactttgca ttgaaaaaag taatactcaa gaaaaaaaaa tattgctaga ggaggaacaa
     3121 aaaaaagaga gagagaatga gcgagtcaga tcacataaag aaagtgttca gaaattagca
     3181 gatgaattag agttctagtt gatagtcagt tctttcttaa ttcgatacac agtttgtcta
     3241 gttacattat agtctgttgc tatttttgaa atagcaacac cattctcctg gtcctgaacg
     3301 atgcttctat aaacaaggcg acgttgagga tcttttgtgt tcgcactgta taacttagga
     3361 cgtcctttat agacgccgtt tgctttagca atctttatcc cttgtgcctg gcgtcgtttt
     3421 gattcagtac gctcttgttc agcaatcatg gccaagattt gaatgattag gtctttgata
     3481 aatttatcca ggagaggatt tccgattgct tcagccatga tcggtaaatt agtgataata
     3541 agtcgcacat ttttcttctt tagataattt actgaatcga taatctcgtc ataattacgt
     3601 ccaagtcgat cgatagcctc tacaataaaa atatcttggt ctctaacaaa atctagaact
     3661 tcctgaaaaa tcggtcgatg agtaatagtc gctccagatt gtttctcgat gaaaattttt
     3721 tcagcaccaa attttctcat ttcctcgatt tgacgttgtt cattttgatc gatcgat
//
LOCUS       DAZUMI010000184         3697 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000184 DAZUMI010000000
VERSION     DAZUMI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.192"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..251)
                     /locus_tag="ACG81C_002824"
     CDS             complement(87..251)
                     /locus_tag="ACG81C_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065925.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            546..1874
                     /locus_tag="ACG81C_002825"
     CDS             546..1874
                     /locus_tag="ACG81C_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5065926.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2076..2909
                     /locus_tag="ACG81C_002826"
     CDS             2076..2909
                     /locus_tag="ACG81C_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5065927.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2925..3662
                     /locus_tag="ACG81C_002827"
     CDS             2925..3662
                     /locus_tag="ACG81C_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5065928.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1235 a          580 c          774 g         1108 t
ORIGIN      
        1 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
       61 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      121 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      181 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      241 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      301 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      361 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      421 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      481 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      541 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      601 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      661 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      721 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      781 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      841 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      901 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
      961 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1021 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1081 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1141 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1201 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1261 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1321 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1381 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1441 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1501 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1561 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1621 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1681 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1741 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1801 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1861 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1921 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     1981 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2041 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2101 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2161 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2221 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2281 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2341 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2401 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2461 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2521 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2581 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2641 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2701 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2761 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2821 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2881 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     2941 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3001 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3061 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3121 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3181 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3241 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3301 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3361 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3421 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3481 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3541 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3601 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3661 aaaaaagagg ttgtgacaga agggggcaac ttcaaga
//
LOCUS       DAZUMI010000185         3675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000185 DAZUMI010000000
VERSION     DAZUMI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.193"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..619
                     /locus_tag="ACG81C_002828"
     CDS             <1..619
                     /locus_tag="ACG81C_002828"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5065929.1"
                     /translation="NDKKVKTIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPL
                     MIDAIGKLIHEGYECELDIYGEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNA
                     AGFVISSDYEGISNSMLEALAIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKL
                     TDAMRRVLLDRESVVRKAQKGIKLRKELTSKNISNIWYEYLNQFF"
     gene            638..2062
                     /gene="wzy"
                     /locus_tag="ACG81C_002829"
     CDS             638..2062
                     /gene="wzy"
                     /locus_tag="ACG81C_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS5065930.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            2108..3613
                     /locus_tag="ACG81C_002830"
     CDS             2108..3613
                     /locus_tag="ACG81C_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS5065931.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFYRRYVVKIISKIIVKIKKI"
BASE COUNT         1255 a          464 c          646 g         1310 t
ORIGIN      
        1 caatgataaa aaagtaaaaa caattataat tcccaaccca ataaaggaaa atttacctta
       61 ttataataaa actacaagta gtaaaaaatt tattactgca tgtagattgg ataaacaaaa
      121 aaatttgccc ttaatgattg atgcaatagg aaaacttatt catgaaggat atgagtgtga
      181 actggatatt tatggagaag ggggattaaa aaccgagctt gaaagttata tcaaccataa
      241 tggattaaca gaatttgttc atctaaaaga atttacaaat aaaatacatg aaaagatgca
      301 aaatgcggca ggatttgtaa tttcttctga ttatgaaggt atatctaatt ctatgttaga
      361 agcactggct attggagtgc cagttatcgc tacagattgt ccagtaggtg gcgcaagaat
      421 gtatttaggt gatagaaatg aaataggtca tcttgttgaa tgtaataatc tagataagtt
      481 aacagatgca atgcgtaggg tacttttgga tagagaaagt gtggtaagaa aagcgcaaaa
      541 aggaattaaa ctaagaaaag aactgacatc taaaaatatt tcaaatatat ggtacgaata
      601 tttaaaccag ttcttttaac caaaaggagg atattgtgtg aaagaaaaaa taaatatatt
      661 gatgttttcg ttaacgttag tcgcgtttac aatattttgc gctttttcat taaatcagaa
      721 ctattggcta aattggcttg gaatattgat ttacttatat gcaatctgta catggcattg
      781 gagaaaagga gattcaatat tttcattata cacgattttt ttcactcttt ttttattatt
      841 tagttatggg caatgcatta tgtgggcatt cggtattgga acagatcgtg gaatcggtac
      901 aacagtagta gcttatggta cggggattat tcctagtgaa tcagatatga taatggtaaa
      961 atggtattct tgtatttcta tgtttgtatt tcacatagga gcactcttat ttacgagaaa
     1021 aaccacacta agaaaagtta gttggtatga atctggagat gctgatgccg atagaaagtt
     1081 tttatttaaa atagggtgta tcatttctat aatagtagta ccgatagttt ttgtttctaa
     1141 attactagag gtacgtatag cattagtcca tggttataat gcattatatt atggtgatta
     1201 tgctacacaa agtggatatt tacaaattat tctatatctt ttttttccag ccttaatatg
     1261 tttattaatt ggtagcaact tttcaaaagt aataacgaga tttgttttta ttatttttgc
     1321 tttatattct ttattaggaa ttatgtcagg agacagagga agttggttgt atagctctat
     1381 tatactcata tgggcataca ctcaatataa aggaaccttt aattataaaa aattaataaa
     1441 gtggcttatt ttagctgtta taggaatata tattctaaac gtaataacga aagcacgtga
     1501 tggtggagga ctaagtaaac ttactttaaa agattttaca tctgtatttg attcagatga
     1561 ttctccttta gttgattctt tttttgaaat gggggggact atgagtatta taactttttt
     1621 cctaataagt ggtaatggaa tatatccctt tgctaatact tatttgacct cactcttagg
     1681 ctctatatca acaagaatgc tatcaatgtt tggaataaag tttgttttat tagctgattg
     1741 gttttctcag gattatttag gaatcacttg ggggactgga ttctcaatga ttgcagaagc
     1801 ctatgtcaat ggtggataca ttgggggtat agtctatatt tttattattg gtatagtcat
     1861 cggtaaaatc ctttctaata gtatagacaa ccaagatatt gaaaatcatc ctatgaagat
     1921 gtttttggct ataactacag caaatattat aatgggtttt cctagagctg cttcatattt
     1981 aattgtgaaa aattttgtgt atggtgtttt aattatattg ttgataaact ttttcctaca
     2041 gcaatttaat aaacgtcttt aatatttaag cttaataaaa agataaaaca agtcaggagt
     2101 tgcaaaaatg gaaagaactg ctcggctcaa aaaaaacact atttgggctc tggtgtttca
     2161 aataacttct attgtctgtg gtatgatctt gctacgtttg atattaacaa agtacggatc
     2221 cgaggttaat ggaatgatta gctctattaa ccaatttcta caaattattt cttttttaca
     2281 attaggagta ggcgctgtaa tgcaatcaac tctatacaaa ccactagcag aaaaggataa
     2341 tttaatgata agtcaaatct tagcttctgg agggaagttt tatcggcaaa ttgcatatat
     2401 tctactagta tacgtgggtt tgttacttgc agtctattat gttctcgttc ctaatgaatt
     2461 tgggagatca tatacaatgg caatgattgc aacattaagt attaattcat ttgctcaata
     2521 ttattttggt attgttaata ctttgttatt aagtgctgac cagaaaattt atattacgta
     2581 tattgcacag acaatagcat ttatttctaa tacagttgca tgtgctttat taattagtct
     2641 gaacacagag atccttatgg taaaacttat tactgctata atttatcttg cttgtccaat
     2701 atattataga tggtatgtgg atcgaaacta taatataaag aggaatgtgt catatgataa
     2761 ggaaccgatc cctcaaaaat ggaatggggt tgcacaacac gtagcttctg ttgtactaga
     2821 tagtactgat gttgtagtgt tatctatatt ttctaacctg atatatgtat ctatatataa
     2881 tgtgtacaat ttagtaatat acggagtaaa acagctattc atggctatga caaatggcgt
     2941 tcaggcctta ctaggggaac tttgggtcag aaaagaaata gaagttcttt acaaaacatt
     3001 tgcatggttc gaatggattg tacatacttt gacagttttt atttttggct gtaccggtat
     3061 actaatcgtt ccatttattc aagtatatac aagaggagta acagatgcta attataatca
     3121 attatcattc gcagctttac ttgtaactgc taacgcactt cactgtctgc gtacacctta
     3181 tcatgtaatg ataaaagcag ctggtaaata taaagaaact caaaatagtt ttataattgc
     3241 ttctttgtta aatttaggtt tatcattaat tttggttaaa ccactagggc tagttggagt
     3301 agcattagga tctctaattt caatcacata tcatacactt tggagtgcaa attataattc
     3361 aaaatatttg cttaaatggc cgatgaaaaa attctataaa cagttatttg taaacatctt
     3421 aactgtagtt ctttcttttg gggcgacatc acaattgaaa atgatgtcaa tcacatattt
     3481 ggattggatt gttctcgcaa ttaaatgtgc agttattgta tttatatgta taatcctagt
     3541 taatgtttta ttttatagaa gatatgtagt aaaaataatt agtaaaatca tcgtaaaaat
     3601 aaagaaaatt taatacacaa taaatgtttt aataaactta ataaaattat atttgccaaa
     3661 attagacaca tatat
//
LOCUS       DAZUMI010000186         3633 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000186 DAZUMI010000000
VERSION     DAZUMI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.194"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(416..919)
                     /locus_tag="ACG81C_002831"
     CDS             complement(416..919)
                     /locus_tag="ACG81C_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5065932.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1278..2303
                     /locus_tag="ACG81C_002832"
     CDS             1278..2303
                     /locus_tag="ACG81C_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5065933.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2626..3504
                     /locus_tag="ACG81C_002833"
     CDS             2626..3504
                     /locus_tag="ACG81C_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5065934.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1194 a          574 c          690 g         1175 t
ORIGIN      
        1 cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa tcaaggagaa
       61 gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc
      121 tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag
      181 attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc
      241 tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca
      301 ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga
      361 ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt
      421 gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt
      481 atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt
      541 gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg
      601 ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa
      661 tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag
      721 gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa
      781 aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat
      841 taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa
      901 attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc
      961 tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag
     1021 aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg
     1081 aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt
     1141 ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac
     1201 taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac
     1261 taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata caagataatc
     1321 cttttcgtat catgaatctg atgctactta tctttattgt attatggtta ggttacataa
     1381 atcatgattt tttgatttca agtttttcat cattagggat ctttactttt ttctattatc
     1441 aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg
     1501 ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag
     1561 ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg
     1621 gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat
     1681 taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga
     1741 ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa
     1801 gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc
     1861 tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat
     1921 gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc
     1981 tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc
     2041 agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta
     2101 gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta tctacactta
     2161 ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa
     2221 gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg
     2281 ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag
     2341 atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa
     2401 attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa
     2461 attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa
     2521 gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa
     2581 gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat
     2641 atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact
     2701 ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat
     2761 gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca
     2821 aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat
     2881 gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag aactatctat
     2941 ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc
     3001 tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt
     3061 ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa
     3121 ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca gaataagata
     3181 agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac
     3241 caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa
     3301 gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac
     3361 gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa
     3421 agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct
     3481 ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga
     3541 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
     3601 caactccttc ggaaataagc tgaaattcac aaa
//
LOCUS       DAZUMI010000187         3610 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000187 DAZUMI010000000
VERSION     DAZUMI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.195"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..1043
                     /locus_tag="ACG81C_002834"
     CDS             162..1043
                     /locus_tag="ACG81C_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5065935.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1056..2666
                     /locus_tag="ACG81C_002835"
     CDS             1056..2666
                     /locus_tag="ACG81C_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5065936.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2743..3402)
                     /locus_tag="ACG81C_002836"
     CDS             complement(2743..3402)
                     /locus_tag="ACG81C_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5065937.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         1009 a          688 c          709 g         1204 t
ORIGIN      
        1 gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac
       61 taaaataata aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat
      121 cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag
      181 tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg
      241 tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga
      301 ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag
      361 atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct
      421 ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa
      481 aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa
      541 aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg
      601 ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa
      661 cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt
      721 ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa
      781 cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta
      841 tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc
      901 aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg
      961 aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat
     1021 tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt
     1081 ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg
     1141 gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag
     1201 gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa
     1261 ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc
     1321 ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa
     1381 aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa
     1441 actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca
     1501 gctgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg
     1561 ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac
     1621 tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc
     1681 ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct
     1741 ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt
     1801 tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca
     1861 ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc
     1921 gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt
     1981 tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg
     2041 acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca
     2101 cgatcatggt agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt
     2161 tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga
     2221 accaacttta ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga
     2281 ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg
     2341 atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg
     2401 gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact
     2461 tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt
     2521 ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg
     2581 tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata
     2641 ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca
     2701 aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg
     2761 ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa
     2821 taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt
     2881 cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata
     2941 gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt
     3001 tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa
     3061 gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc
     3121 aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc
     3181 tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc
     3241 ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc
     3301 cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt
     3361 gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa
     3421 acagctattt ctaataaaga aacataactt cccttattcc aactgataat tttcttttat
     3481 catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc
     3541 ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata
     3601 tttacttttt
//
LOCUS       DAZUMI010000188         3546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000188 DAZUMI010000000
VERSION     DAZUMI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.196"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..1855
                     /gene="yfcC"
                     /locus_tag="ACG81C_002837"
     CDS             116..1855
                     /gene="yfcC"
                     /locus_tag="ACG81C_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS5065938.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2012..3196
                     /gene="iadA"
                     /locus_tag="ACG81C_002838"
     CDS             2012..3196
                     /gene="iadA"
                     /locus_tag="ACG81C_002838"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5065939.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3307..3510
                     /locus_tag="ACG81C_002839"
     CDS             3307..3510
                     /locus_tag="ACG81C_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5065940.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1128 a          591 c          822 g         1005 t
ORIGIN      
        1 aaagtgattt tctaataaat tattcatgtg gaaatgtcat ttttactgta taattcattt
       61 gattatccat tttttattca tcaaaattac tgaaaaaaga aaggaagaaa taaatttgaa
      121 gaaaacaaat tcttcaggaa aaccgaaacg cgacttcact aaattatcga caccccatac
      181 gtatgtcatt atttttggtg tagtcatctt tgcatggata ctgacttttg tcgttccagc
      241 aggaaagttc agcacgcaag atattgaata caaagatgca aatggcgaaa caagtactcg
      301 tactgtttta cgtcaagatt ccttccgtta tgcttacgag ctggataagt cgtatgtgtt
      361 tgatcaatta gaagaactgc aagatcatcc agcagaacgt gaaaaactgg atgttccgga
      421 aaaaggattg gaaaaagtca ttgctgacgg agaaaaaaat cttacgcagg aaaaactaga
      481 tgaaatctcc ttgacagatg atgtgcttta cgatcagtac ggcgaaaata tctatgatac
      541 ttctaaaaaa ttgcataaaa cagccaaaat atggggaaca gatgattttg gcggctttgg
      601 tttcttaaat tttgtttttg aaggactagt atctggagat aagtatggat ctgctgtagg
      661 gatcgctgca ttgatccttg ttgttggagg tgcttttggt attattatgc gtaccggagc
      721 gatcgacgca ggtatctatg cgtttatcag taaaactaag ggactggagc gtttagcttt
      781 accgctatta ttctttgctt tttcattcgg tggcgctaca ttcgggatgg cagaagaggt
      841 aattccattc tcaatggtga tggttccatt tgtcattgcg ttaggatatg actcgatcgt
      901 agcagtaacc gtgacttatg tcgcatctca agtcggaaat gcgacctctt ggatgagccc
      961 gtttagcgta gcggtggctc aagggattgc tggaatcccc gtgttgtctg gtgcgacatt
     1021 ccgattgatt atgtgggtag ttgttactgc attagccgct ggttatatga tgatatatgc
     1081 agaaaaaatc cgtaaaaaac cagaaagttc gttgacttac aaatcagatg attatttccg
     1141 ttctcatatc aaaaagacat ctgatgaaaa taaaaaattc atgcttgggc ataaattgat
     1201 cttattagag atgttagttg tccttgtctg gatcgtttgg ggcgtgactc aaaaaggcta
     1261 ttacattcca gaaatcgctt ctcaattctt tgtgatggga ttagttgctg gaatcattgc
     1321 tgttcttttc aaattagatg gaatgaaagt caatgacatc gcttcttctt tccaatctgg
     1381 tgcagctgat ttagctggta cagcaatcgt agtaggtatg gccaaaggga ttcttcttgt
     1441 cttgggagga tctgatgcat ctgttccttc agctttgaat acgattttgc atgggatcgg
     1501 gacggcgttg accggtgttc cagctgtcat tggtgcttgg gcgatgtaca tcttccaaag
     1561 tttgttcaac ttggttgtta catcaaattc aggacaagca gctttgacta tgccaatcat
     1621 ggctccactg gcagatttag taggtgtatc cagacaagta gcagttcttg cttatcagct
     1681 tggttcaggt tttatggacg cttttacacc cgtatcagcc agcttgattg gtgtgctagg
     1741 tgttgcacgg atcgaatggg caaaatgggc aagattccaa atcaagatgc aaggattcct
     1801 gtttgtttta ggaactattt tcatcatgat tgccattgca ataggtctgc agtagtactg
     1861 gagacctgta aataaaaaag taatccaaac agtagataag gactccaaga accgtcattt
     1921 tacggtaaaa tcaaaggagt aaagatccta tcgtttggat tattttttaa gatcctatca
     1981 tcagttatac ttaaagaaag aggaaaaatg tatgaaaatc attcgacaaa ttgaagtgtt
     2041 tgcaccagat tacttaggaa aaatggatgt actgattgcc ggtgggaaga ttttggctgt
     2101 agaagaacag ctagaaggcg gctacgaagg agtggaagta gaagaacttt caggtgaggg
     2161 aaaagtacta accccgggtt tcattgactg tcatttccat attttaggtg gaggaggaga
     2221 aggcggttat caaaatcgaa cgccagaagt cacattgagc caattgacta cagctggtgt
     2281 aacgacagtt gtcggttgtt tgggaacaga cggagaaggt agagatatga ctgcgttgat
     2341 cagcaaagcg aaaggtctgg aagcagaagg aatctccact tatgtatacg aaggttcgta
     2401 ccgattgccg gtaaaaacag taacagattc tatcatcaaa gactttttga caattgataa
     2461 aattatcgga atcggagaaa tcgcagtatc cgatcaccgt tcttctcagc caagttttga
     2521 agagttcgct cgtgcagtag cagatgctcg tgtaggaggc atgttatccg gaaaagctgg
     2581 gatcatcaat gtccacttag gcggaggaaa aagaaagcta gagctattga cacgaatcgt
     2641 agaagaaaca gaaattccga tcacacaatt tttgcctact catgcaaatc gcacaccaga
     2701 attatttgaa gcttgcgtgg catttgccaa acgtggtggc acgattgact tcactgctag
     2761 cgaagatcct gatttttggg agaagacaga tggtgaagtt cgcttcagca aagcactgaa
     2821 acgattgatc gaggaagatg tttctttaga taatttcacg atgacctcag atggacaagg
     2881 aagcttgcct tattttgatg aaaacaatca tttcctaggg ttaggagtag gaagtgccaa
     2941 ggcattgcta gtagggatca aagaagctgt gcaaaaagaa agtatccctt tggaaatcgc
     3001 cttacgagct ataaccagta atccagcacg tatattgaaa ttggataaaa aaggaaaaat
     3061 cgaaattggt gctgatgcag atctgtgtat cttggataaa gaaacgcttg atattgatac
     3121 agttatcgct aaaggagaaa taatggtcca agaaaaagaa gtcaaagtat gggggacttt
     3181 cgaaaaaagt ttttaatcaa cagctctaac tatctttttt agaaaacgag cgttctgttt
     3241 gtctgtatct gttgttgatg ttaccctgaa ggtagatcaa taaacaaact aagaggagga
     3301 agcacaatgg cagatttaaa aggacgttct aaagatatga aagataaagt agtcggtgaa
     3361 ggaaaagaag catacggcaa agcaacagac gacaagagca aagaaacaga agggaaagca
     3421 caatcactag gtgctgacat caaagaaaaa gctcgaaatt taggcgacga agtcaaagac
     3481 aagttttctc gaaaagacga aaaaaaataa aaaccactta ttactaattg aatcaagcaa
     3541 ataaaa
//
LOCUS       DAZUMI010000189         3518 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000189 DAZUMI010000000
VERSION     DAZUMI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.197"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2270)
                     /locus_tag="ACG81C_002840"
     CDS             complement(<1..2270)
                     /locus_tag="ACG81C_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFS5065941.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESLSESFNEARSSEPSITQESQIER
                     QEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVRVPRASIGLIEF
                     IRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITFEVAFFQSGGTE
                     PNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGNLGNLLIDGQDV
                     TNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREPISGGVRIAEVT
                     NPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYALEVTDVDLFNE
                     GRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNVINLNENELTYT
                     LHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTS
                     FTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLA
                     GAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFIN
                     EYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGETLQTGLTTGEDG
                     KLAIDGLAPGAYQLVET"
     gene            complement(2942..3210)
                     /locus_tag="ACG81C_002841"
                     /pseudo
     CDS             complement(2942..3210)
                     /locus_tag="ACG81C_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          983 a          783 c          556 g         1196 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtacattaaa agactcactt gtgctttctt ctggtacact gatttctatt
     1921 gagaccgtat ttgattgaat atcaccgctt cttgcggtca attcgtattt acctgaatgc
     1981 tgggaagaat aagtaatcag acgttcgtaa tcattgagtt gcttcacttc cccttcaatc
     2041 aaatcctcac tcttatcaat gaccaatgca tttagcaggt ctggtgttcc tctgactcgc
     2101 aactggaact gctcacccaa agcagcctcc tcttgatcaa cttcgatcgt cagcgaacgc
     2161 tctggctgtt ctgctaaaga ctcagctaga accgttatgt tcccaaattg attgatcaca
     2221 ataagcataa ctagaaacat acttaacagt ttatgaaatt tgttcctcac tattactccc
     2281 ccttataatt ttgactaaac aacaaggtac ccgataattt tctaaaaaga ttccccaact
     2341 caaaatggct gcgttacctc cctcgtattt tcggttcaac tcaagcgttg caatgtaagc
     2401 tgtaaacaaa gctaatgatt gccgattgtc ttctgttgat tgattcgata gctttgtatg
     2461 acttttagtt gtccgtgtgg cttaaccata attatgattt cacgattcct cctgccagtg
     2521 ccttgcacaa gctggagcgt aaccctaata gttattttgt taatgtactt atatactttt
     2581 atacttttcc attagaaaca atcactaaat cgttgatacc aaggtcagac tctagaattt
     2641 catcagtgag tccctgtatt ggttaacctt catcaatccc aaaaaaatat atcaatgctt
     2701 agcatcaaaa tgaatatata gtttagaata cttgggattc atcgataact gatcgcttat
     2761 ttggccatat tatttggcac ttggttcaac taaacgcatt taggatgttc cttcattctc
     2821 gtcatggatg ttctcaaacc gtcattacta ataaactgtc caccgaatcg atggttcatt
     2881 tcttgcattt tccacgtcca attactcttt ttgctcttta tttgggcgta gtttcacttt
     2941 cttaacttgt atcgtcttcc atcaactcct taatcatctt cttagcttta ttggctcggt
     3001 ttccttgtaa aaggtatcta caaatagtga caatctgtat taaatcttca tcattttctg
     3061 taatgtcaat aatttctata ttctgtacca tatattgcaa agggtttcac taagttcata
     3121 accgaagcga attaaacaac ttttgtatag aataactacc ttttctattt ttccattggt
     3181 aattctatct attaattggt ttaatccttt tgttgtagtt gattccacta ccaagatgat
     3241 tctctcgaat tttaaagctt gaagatgtat tgctattttt gctaactggc gtatttgcaa
     3301 ctatttttcc cttctattct tccttcattg attcccctct aaaattacta tccataaaaa
     3361 attgcataaa tgacaatagt atttatatta gtcatactgc tttttaacac agcaaattcc
     3421 agaaaaacat tcgttacata tttgtgaatt tttccaaaaa ataacatttt tataaaacga
     3481 cgaaaaataa atcactacca gtcaacttct ataatatt
//
LOCUS       DAZUMI010000190         3469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000190 DAZUMI010000000
VERSION     DAZUMI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.198"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1314
                     /locus_tag="ACG81C_002842"
     CDS             <1..1314
                     /locus_tag="ACG81C_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS5065942.1"
                     /translation="VIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPTDNWSGL
                     LTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFASILTVTG
                     FSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFGMRFPLL
                     IGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLRLFSYSI
                     VENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTTDFIANS
                     GIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSFPILVGI
                     FIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHNVSTLHG
                     GLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1280..1630
                     /locus_tag="ACG81C_002843"
     CDS             1280..1630
                     /locus_tag="ACG81C_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065943.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1722..2141)
                     /locus_tag="ACG81C_002844"
     CDS             complement(1722..2141)
                     /locus_tag="ACG81C_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5065944.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2142..2486)
                     /locus_tag="ACG81C_002845"
     CDS             complement(2142..2486)
                     /locus_tag="ACG81C_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5065945.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2836..3432
                     /locus_tag="ACG81C_002846"
     CDS             2836..3432
                     /locus_tag="ACG81C_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5065946.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          899 a          637 c          630 g         1303 t
ORIGIN      
        1 gttatttata ttcatcctca aaaacgaatc gtttcacaga aaagaaagta tttttatctt
       61 ggttttacag gtgttttttt tctttttatt ggcttgcttt ctaatactcc tactgataat
      121 tggagtggat tgctaactat tcttacctcg ccaagcaatc ttttgacaga ttattttgct
      181 ttaggtgttt tcggaagcgc attcataaat gtagggattt tgaccttact ttctgtgctt
      241 ttagcttatc ggcacaaggt gattttaaac ggtcccttat ttgcttcgat tctaacagtt
      301 accggtttct ctttttttgg caaaaacctt tataattcta tctcaatcat tttaggtgtg
      361 tatctatacg ctgtatttgt caataaacct ttttctcagt acatcatgat cggactgttt
      421 ggtagcgtat tatcaccggt cgttagttat atcacttttg gtatgcgttt tcctttactg
      481 ataggtattt tattggggaa tttagctggc atagcaattg gtatacttct tccgccctta
      541 gctgctcaaa ctttagtttt ccatcgtgga ttcacacttt ataatattgg attcacctct
      601 gggttgattg cgatgacttt tactgctgtt ttgaggttat tcagctattc aatcgtcgaa
      661 aatacattgg ttttcaatga atatcacttt cctctcattt ggattatttt tggctttttt
      721 tcgctgacag tcggaattgg tttctattat aattcctttc ggctttcggg gattcgagaa
      781 attttcgatt catctggaaa attgacaact gatttcattg ctaattctgg tattggtgct
      841 actttgatca acatgggctt agttggtctg atgctgtctt cttatgtttt gctggttggc
      901 ggtcagttaa atggacctgt catcggtgct attttatctg cggtgggttt cagtgctttt
      961 ggttgtcatt taaaaaacag ttttccgata ctagtcggaa tatttattgc ttctttgttt
     1021 ggaacttttc atgagatcac cagtactggc atgcttgttg cagctgtgtt tgggacaggt
     1081 cttgcgccca taagcggttt ttacggcagt ttttacggtg taatagctgg tgtgctacat
     1141 attgctcttg tccataacgt cagcactttg cacggaggac taaatctata taacagtggt
     1201 ttttcaactg gtttcgttgc aggtatcctt gtaccgatac tagataactt taccgctgtc
     1261 agaaaggaaa aaaagacact tggaaaaaga attatcaaaa agaatcatcg atgagcttct
     1321 tacttattta tattcccatg aaataactga aatacgtatt ggagtagaat ttgctgagga
     1381 agggcttttc atagagatac agggtacggc agaaactcag ccagaagatt tagatgtact
     1441 tgctgatttg ctgaacaatc ctcgggaccc tagtatggaa atctactatc aagaactatt
     1501 aggtaatccc cgacatgatc gggaagatta tcatctgtta ggtagtttga ttgatgacgt
     1561 ggaaatcatg tatgcccagc cgattatcaa tattaagatc tttcgacgtg cttctcattc
     1621 ctattattaa ccctattttc tatttataaa agactgaatc aaaaaagcag ctcctgaaag
     1681 ttcgattaaa atctactttc aggagctgtt caagcttgac attattgatt ttccaatcct
     1741 tcatacactt catctgttac aggttctaaa aactctgaat taccagcagt gatcgccaaa
     1801 tgttcaaacc aactgtcttt tgtcgcacca tgccaatgtt taatcccttt gtgcgttacg
     1861 acgacatctc cagccattaa tcgtcttgct ggctttcctt cttcctgata ccagccttct
     1921 cctccggtca caagtaagat ttgatagccg tctgtatgga catgccagtg attgcgacaa
     1981 cctggttcaa aagttacatt acctacacca acagaaactt cgggatcatt cactaacata
     2041 ttcagatagc tttgcccggt aaatacttga gcgtaagctt cgtttttttc acctgctgga
     2101 aaaatcacgc cattgtgtat ttcttcgtgt tttgtcatca tttacgcctc tttcttatct
     2161 tcgctatttt cttctaaata gatttctttt gctaaactaa aagctgacca agcttttggc
     2221 cattctacat aaaaagctaa atgggtaatg atttctgcta tttcttcact agtaatgcca
     2281 ttttgcttgc caatcttcag atgagctggc aattgttcaa aatttcctcc tgaaattaat
     2341 gcagatatgg tgatgatact acgctgatga gcagataatt cttctctgga ccatacttgt
     2401 ccaaataaaa catcatcatt taattctgca aattttggtg caaaatctcc taattgatct
     2461 cgtcctgcgg tttgtttttc tgccatttct cttattctcc cttcacgact gttaggtgat
     2521 tcaactatgg ttctaaggca actttatttt ctattgtttc ttcgcatttc attttaaatg
     2581 tagtctttca gcctgtccaa tgcttagttt attcagtcga tatgcttttt aagcatagtt
     2641 gctaacgctt tttttatttt aaaaaaaaca agttattaaa taagtcttta gttggatgtt
     2701 tttttgagat caatctttta tacttatgat gaacttacaa aattgtttgt tttctgtcat
     2761 atcaaaaaat ggcgcagtta gttgcttttc tagtaaagta tagttaacaa agaacctcta
     2821 aggagttgat gaataatgaa tattgctgcc tctcgaaaac tgataaattt catatccatt
     2881 atcggattag gcctatcaat cgcgctgacg atttatttta ttaatttagg tgttttcaaa
     2941 gatttgaatg cgttaagagg attagtaggt gattccatca ttttaggacc gattattttt
     3001 gtcttcatcc aaatcctgca agtagttatt ccaatcattc ctggagggat cagtactgca
     3061 gctggtgtcc tgatatttgg cccttatgct ggatttatct ataactatat tggaatctgc
     3121 attggttcga ttatcatttt tctgctaggg cgtcgttatg gaaagccatt catactttcc
     3181 atgattagcg ataagacata taacaaatat attggctggc tggataatca aaaccgtttt
     3241 gagaagctgt ttgctctagc tatttttctt ccagttgctc ctgacgatgc gttatgtttg
     3301 atggctggat tgacaaacat gtcagtgaaa cgatatacac taatcatttt gattgctaaa
     3361 ccactgtcga ttttcttata tagtatggcc ttgatttatg gaggtcaata tctaagtggt
     3421 cttcttggat aatatttatt ttgtaaaatt agtcattgat tgacaatgt
//
LOCUS       DAZUMI010000191         3278 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000191 DAZUMI010000000
VERSION     DAZUMI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.199"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG81C_002847"
     CDS             complement(554..2167)
                     /locus_tag="ACG81C_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5065947.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACG81C_002848"
     CDS             complement(2308..3216)
                     /locus_tag="ACG81C_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5065948.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1061 a          560 c          574 g         1083 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaa
//
LOCUS       DAZUMI010000192         3235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000192 DAZUMI010000000
VERSION     DAZUMI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.200"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..671
                     /locus_tag="ACG81C_002849"
     CDS             <1..671
                     /locus_tag="ACG81C_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5065949.1"
                     /translation="TAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKN
                     GVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWI
                     KGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISH
                     LPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGT
                     LYAT"
     gene            661..1401
                     /locus_tag="ACG81C_002850"
     CDS             661..1401
                     /locus_tag="ACG81C_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5065950.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1398..>3235
                     /locus_tag="ACG81C_002851"
     CDS             1398..>3235
                     /locus_tag="ACG81C_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5065951.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1212 a          395 c          628 g         1000 t
ORIGIN      
        1 atacggcata caatacgtgg atttctataa aaaataccat gattaataca tggaataaca
       61 ttaaggactc tttctggaat attgttactg gaattgttaa ttctgctgaa aatgcatgga
      121 caaatctaaa aaatggtgtt tcaaatgcta ttaatcgggt aaaagaaatc tttaattcgt
      181 taagggaagt caatttattt gaaattggca agaacatcat tgatggactt ataaatggtg
      241 taaaagaaaa gtggaatgct ttgaaagata ctatcaaagg tattgcaggt aatatcaaaa
      301 attggattaa aggagcttta gatattcatt ctccatcaag atggatgcgt gatatggttg
      361 gtaaaaatat tgttcaaggt attatcgttg gtattgataa agagcaaagt aagttagatc
      421 aaaccatgac aaatttagta aaaacaccgt ctgttcaacc tgtagtaaat ggaataagtc
      481 acctaccaat tacacaaaca aagcaaaata tatctgctag tgaaacaaaa gaaattcatt
      541 tacatttaaa tgtttatgga gatttacctg attcaatggt tcggaagatt accacaaaaa
      601 ttaaaacaga attgacaaga caaatgaaac gagatgctgg tgcagtagga ggaacattat
      661 atgcaactta agagaggaca attttttatt aatcaacatt attcttctga atttaatgtg
      721 tatattcaaa acaggcctgc ttctgtttca gctagtcgag taattgaatt gagagagcga
      781 gaaggaaatg attctatcat tattgataaa ggctattaca aaaatgtgac tagaaagatt
      841 gagtgttact ataaagcacc ttcaattgat atggtgcagg agtgggaaga tcgaatcact
      901 gaatggttag atatgtcgtc ttatagtgat tttattcttt actatgatga acaatatatt
      961 tatcaagctg tagtaataga aggacctgaa tttaaaggaa caagaaaaac aggaaatatt
     1021 gtcccttttg aatttacagt gagtattcga ccatttaagg aaaattacaa tggtagattt
     1081 actattcgac aaacagaaac ttttgaaatt tataacccag agaagtattc ttcaaaaccg
     1141 cttattaaat tgagtggttc tggagatgct tctttttata ttaataagga taaatatgat
     1201 ttgaaatcgt tggacagaga attatttata gattctaaat tagaagaggc ttatcgaaaa
     1261 ttagatggta atttagaaca tcaggatcag gtcaccttat ttttagattt tccattttta
     1321 tatcctggaa aaaatgaaat taaatggacg aaaaatattc attcatttga aataatgcct
     1381 aggtggtgga gaaaagtatg aaaccaagaa tatatagtcc tactgaaaca gatttctcaa
     1441 cgaatggctt aggaatttta aaggattgta caagatgtgg aatatatgaa gtagctaatg
     1501 gaaaatatga attagagttg gattatcctt taggaactag atttgatgaa tattttgaaa
     1561 atgactatca aataaaagca aagccaaacg atcaagaaga gtatcatatc ttttttattg
     1621 atgataaaga tatagatact tttttaaata cagtaactat ttatgctcag agtcgtacaa
     1681 atcgacttgg aagacgggta gtcactcttg tggaagtgga ctctaaaact ggtcaagaag
     1741 cgatatcaat tattgaaact aaaatggata aaaaatctga catacgactt tattctgata
     1801 ttacggctgt ttctagcaca atctttgaag cgagaaatgt tttaaattgt attgctggtg
     1861 aacaaggatc attgcttcaa tattggggcg gagaaattaa acgtgaacca tttaagcttt
     1921 ctttgttaaa gcgaagaggt cgcaataatg ttggaacgat tcggtatgga aaagatatgt
     1981 ctggtttgaa ggtcaaatta gattggacag gtataaaaac aagaattatt ccatatgctg
     2041 atcctcaaag tgaagtaggt acgactaatc gaatttatgg ttcgccagta gatagtgcct
     2101 atattaataa ctatcctgat gtctatacag aacatgttca gtttacagaa gaacaaggag
     2161 taaaagacat taagagctta aataaaatag ctaaaaatta ctttaaaacg atcaatcctg
     2221 gctgtgataa gcctaaagtt tctattacgg ttgaatttga taagttgact gatagtgaag
     2281 aagcgaaaga atttgcgaag attagaaatt atggtctgtt tgatacattt aaaatatatc
     2341 ataaaaaata taatatttat cttgaatcaa aggttagtgg actacaatac gattctttat
     2401 ctgaaaaaac tttgaaatta gaagcaggag acattcaagt tgctttttat cagcaacaag
     2461 ctgtaactat tcaggataaa ttaaaagatt atgcaacgaa taattatatg agtgatttta
     2521 atgattatgt ttcttcaatg attacaggtc aaggcagtgc aggaggatat gtagttttat
     2581 ggccaaaaga aaaaccttcc aacatcttta ttatggataa tccagattta gaaaaggcaa
     2641 gagaagtact aagaatgaat aaaaatggga ttgctttttc taagaaagga tggcgggggc
     2701 cttttaattc agcttggaca ttagatagta tatttaatgc caattttata caaactggat
     2761 taatcaaagc agatatcttt caaaattctt ttaacaagac tggagatgta ttgaaattag
     2821 ttaatggact actccaaatt tggaacaaca aaaagaaaat aatggagtta actaaaaaag
     2881 gaatggaatt ttggaattct aagggttcaa ttggaacgat tggaacaact gattctgttg
     2941 gtaatccttt tcctggggct tctactccaa cccctattga agataattct ttagttattc
     3001 gtacaaatgg agacggcaaa tatattttga tttctcctaa agtaggaaaa ggattagttt
     3061 tattaggaaa tggtaaagca atttattttg gagacttaga tgtacaagga aaacttacag
     3121 ttaaaggaaa agaaataatt cctggacaag gtggtggtcc atcgggaggt ggagaatcta
     3181 ctggtgggta tcctagtgaa ttaaaaacag atgcagaaaa gagagcttgg agaat
//
LOCUS       DAZUMI010000193         3199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000193 DAZUMI010000000
VERSION     DAZUMI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.201"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG81C_002852"
     CDS             301..585
                     /locus_tag="ACG81C_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065952.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG81C_002853"
     CDS             588..1004
                     /locus_tag="ACG81C_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065953.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG81C_002854"
     CDS             complement(1141..1860)
                     /locus_tag="ACG81C_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5065954.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG81C_002855"
     CDS             2522..2608
                     /locus_tag="ACG81C_002855"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5065955.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACG81C_002856"
     CDS             complement(2910..>3199)
                     /locus_tag="ACG81C_002856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065956.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAZUMI010000194         3198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000194 DAZUMI010000000
VERSION     DAZUMI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.202"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..332
                     /locus_tag="ACG81C_002857"
                     /pseudo
     CDS             57..332
                     /locus_tag="ACG81C_002857"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            359..658
                     /locus_tag="ACG81C_002858"
     CDS             359..658
                     /locus_tag="ACG81C_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS5065957.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            832..1383
                     /locus_tag="ACG81C_002859"
     CDS             832..1383
                     /locus_tag="ACG81C_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS5065958.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1529..1822
                     /locus_tag="ACG81C_002860"
     CDS             1529..1822
                     /locus_tag="ACG81C_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS5065959.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1815..2111
                     /locus_tag="ACG81C_002861"
     CDS             1815..2111
                     /locus_tag="ACG81C_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065960.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2186..3184
                     /locus_tag="ACG81C_002862"
     CDS             2186..3184
                     /locus_tag="ACG81C_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5065961.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1196 a          443 c          646 g          913 t
ORIGIN      
        1 ttatggcaaa ggagggaaaa gaaatgtttc aaaataaagg ataacgttat gtcacaaagg
       61 gagtaatgga cagtttgccg gtggaattac aagctttatg ttggaatcta atcgatcaaa
      121 acgttcaaaa gcaatttcct ttagactatc ttcaaatttt tgaattttct acggaaaaag
      181 gaaatcaaaa gttggttcat cgtcaagaag aaccggaaga aagaaaagag tatctgattt
      241 cacctaagct tcgtttgaaa tcagtgtctc aaaaaatatg ggccattgat tcgggtgagt
      301 atcaaacgat gttattacca gaagaatact agtatctaaa agaaaggaac gaaaggactt
      361 gttagaaacg gctatcgaag tactggaaaa gtgtgcacag ctggtcactg cttctgaaga
      421 gtggggctat gaatccgtta cgatggaaaa agaggaaatt gaaatgggta tgcttcctga
      481 agatgttcat ctaccacgtt tagtgatgac acatctttat atttattgcg caccagaaga
      541 tggaaaagac tatgtagttt atttcattac agatattacc agccagcgag aatttgttcg
      601 tgggttactg gtagaaggac gattggtttg gagtcagatt ggaggaacgg atgaataaga
      661 tcgattattt gagaatgatt gcgaaatgtt tagaagatat ccttaccgcg attcatcaaa
      721 ttctgaagaa aaaaggagaa agaatatgaa atattttcaa ctagtattga cacttataac
      781 ggcaatggta attgaagtgc ttgaacaaaa aatagcatag gagaaaaaga gatgattgaa
      841 aaagaattag tgaccacaca aacagaagtt tggcgagatc ctcatcatac ccatcgctat
      901 gttttcaaac gacaatgggc gatcaaagga aaagaagcgg aagataaatt agcagtagtc
      961 attactattc gtccaacaga tacagaacca tttacgaatg atttgagtat gttattaatc
     1021 gaaaagaata tccgacaatt aggatttact ggttttattg ctgtgaatct tttttcgtac
     1081 acaaaagcga aaagtcctcg ggtatttctt cgaggaaatg atgaacaatc tttggaaata
     1141 ttaacgactg tttttacaga aaagaaaatc aataccatta tttttgcttg tggttcgatt
     1201 acagcgacga gtcaagtggc ttatgaacaa gcaaagacta tttacgatca attgagtgcg
     1261 aaacaaaaga agatgaccaa agtattgata aatgcagaag gtaagttatc ccacccacta
     1321 agtattcatg ttcgtaatga atggcaattg gcagatagtc aattattatt tcaagaaaaa
     1381 taacatttta ttttctcatg ttacttataa gtagttttac atgctttttg actttaaaaa
     1441 tacatgtgat gatacatgtt atatgaatta tcaatgtacc cagtaatatg ctattttcat
     1501 tatgtaaaaa ataacagggg gtgtgtagat ggagattgat gaaacttgtt tgagtaactc
     1561 ttataaaagt atgaatgact tttttgagga aatggaactg gattcagaat tgctctatca
     1621 attttatctt gcttttcgag ggcaacagat ttcttttcca atgaaaatgt acgatcgtga
     1681 gttagttaga aaaagaatcg aaaacatgat agaacaggaa aaaactgtag atatccgtca
     1741 attgacagaa gtttatggtt tctctgcaag atggattcaa aaagtaatta aacaaaaaga
     1801 aaggagacga gtacatggct aagctgattg tattaggtgg tttaatttac ggtggatatt
     1861 ggttatatca atatatgctt gcgcatccga atatagataa aggaaaagta attaagacag
     1921 ttggggtaat catagttgga ctcatagctc taactaagaa aaataatact aatagcccaa
     1981 aaataatgag aacttatcgc tgtagaggat gtggaaggga aatttctact agttttaatc
     2041 catctgagag tcctcaagga agagaccgct gccatacggg ctatcgacat tattgggaaa
     2101 gaatcgatta aaagattacc atttagatca attaattgtc tgatagaata agtacgtgag
     2161 ggagaaattg aaagggggaa atataatgaa aaaatggctg ttattactaa taggaatggg
     2221 aattttattc actggttgta catctgagaa tacgaggact aaagcgacta tagaaactga
     2281 tacaacaatt gagactaaaa aaagtaaaac gcaatcaact gaacaaacta caacagaaat
     2341 gaagcaatct gtggaaacaa gtgcaagttc aatcactgaa tcttcttctg aagaggttaa
     2401 gaatactttg tgggatacga ataaagcttc caaattagaa acttttgtca cacaatgggg
     2461 aaagacatta ggtcaagaat ataaaagcta caatcttcag aataatgtaa gtctgtatgg
     2521 cactccttta ccacaagccg tgattaatgg cgattggaaa atggcaatca atgaagcacc
     2581 agttaccgta caatggtcag aagatggaac tggtcatgca gatttcaatt tagtagcagt
     2641 ttattctgat gtagaaactg gagaatattt agggcagcat gtttactttt tcggttttca
     2701 aaatggacaa cctaaagttt atttaacgca acaaaatcaa ggtaatgaaa ataattatct
     2761 gtactttaac gaaactcaaa atcaacagtt aaaacaaggt ttcagtgata ttgtgaatgg
     2821 acaaactcct caaacaccag tagtagaaga atctactcaa caagcaacaa ctacaacgac
     2881 acaatctaca agtagttcag gaatttcaag ttgggaagct gctaaagaat ctgtgctaaa
     2941 taataaagct ttatggttgt atgattctcc tgataaaaat gtatcaaata ctaatttaat
     3001 tagcacaaat gaatatatga aagatctaat gcaagatgaa aatggaagct actattcaat
     3061 taatattgta acggatgatg atggaaacac gaccatggct ggtggaaaac aatttcgtgt
     3121 ttatacagat ggaagaattt ctcaacgtat tggaatgact aacaattttc aaatgattaa
     3181 ataattgaaa atattatt
//
LOCUS       DAZUMI010000195         3197 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000195 DAZUMI010000000
VERSION     DAZUMI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.203"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG81C_002863"
     CDS             75..503
                     /locus_tag="ACG81C_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5065962.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG81C_002864"
     CDS             490..732
                     /locus_tag="ACG81C_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065963.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG81C_002865"
     CDS             complement(1032..1247)
                     /locus_tag="ACG81C_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065964.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG81C_002866"
     CDS             1354..1668
                     /locus_tag="ACG81C_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065965.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG81C_002867"
     CDS             1772..2950
                     /locus_tag="ACG81C_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5065966.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1049 a          484 c          640 g         1024 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggt
//
LOCUS       DAZUMI010000196         3126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000196 DAZUMI010000000
VERSION     DAZUMI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.204"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1014)
                     /locus_tag="ACG81C_002868"
     CDS             complement(<1..1014)
                     /locus_tag="ACG81C_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5065967.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRT"
     gene            1172..1501
                     /locus_tag="ACG81C_002869"
                     /pseudo
     CDS             1172..1501
                     /locus_tag="ACG81C_002869"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1549..2949
                     /locus_tag="ACG81C_002870"
     CDS             1549..2949
                     /locus_tag="ACG81C_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5065968.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          888 a          580 c          619 g         1039 t
ORIGIN      
        1 tgtacgaatc cttcggcgga tattttcagg aaaagacaaa atttgaatag catcttcaaa
       61 gccgttttct aaaatgtcgc aagcagcact taattttgaa tcacaaccat atttttccaa
      121 gaagtgctct tttctttcac gtgtcaattc aagttcagag gctttaaaga tactcttcaa
      181 ctcgtctttt acatcagaag aaactttctt tggcagctta tcaaaaatat ttctcaaaaa
      241 gtgggcttgg catctttgcc agctcgcacc taaaaagttc ttgcgaacag ccttaactaa
      301 ccctttgtgt gcatcagaaa tgaccagttt tacaccgaac aaacctcgtt gaacgaacga
      361 ttgaaaaacg gtatcccaat tatcttccga ttcgccgtct tgaacatcaa aaccaagaat
      421 cgtacgtcgt ccatcagaat caatgccgat accaatatat acaccttttg agacaatacg
      481 atgattttcc cttactttca tatacagggc atcacacatg aaaaatggat attccgagcc
      541 ttctaggctc cggtttctcc actcaaaaac aaatggatcc aactgtttca tcagatttga
      601 aacgaatgat ttagaaacag tcgcaccatc cgttagcagt tcaactgttt tagttacttt
      661 gcgagtggag acaccagaaa tgaccatttc aatcatggca gcgatcaaag ctttttcgct
      721 tcgctgatat ctttcaaaga tagtaggact aaacttaccg tctctggttc gtggaacatg
      781 aagttctaac gtaccaattc gagttgtaaa cgaacgatcg tagtaaccat ttctttgtga
      841 ttgccgttct tctgaacgtt cataggcatt tgcctgaata tattgatttc gttgttcttc
      901 catcagctga ttaaacatga tagtcataag tgtttttgcg gtatcattcg caccgctttc
      961 gtttataatt gcttgaattt cttcgctttc aaaagtaaaa tgtacttgag tcattgatat
     1021 cagtcctatt cgagtatgtt ttctgcaaaa aacattgtac tcctaactga gatcaatggc
     1081 ttttttgatg cttcactttt acacaattat atggacgtaa tcaaaaaatg tttccacaga
     1141 ggacttcttt aattattgtc gattagagga aagtggtgta acagtcacta gcggtacaga
     1201 tttaccgctg tttattactg atattcctga atcgattata agagcagtat tccgtcgttt
     1261 tcctgaagga gaagaagtct gggagaaaga aacaagcttc acgacaaccg agctgctgaa
     1321 aagttttaca agcaacagct tcaaagtaaa tggtttatcg gagtacggag aaataaaagt
     1381 agggaaaagc gccagcttag ctgttttcag cgaggacctc tgtactgatg actggcaaaa
     1441 agtgatggct gcggaagtta tcgctaccta tcttgatggt gacttagttt acaagaaata
     1501 atgcttttcg atataacgtt ataaaacgaa ttaaagagag gttaaaaaat ggaaacaatg
     1561 aaaagaaaat tcaccttccc acatagttat acattgattt ttatcttaat tattttggct
     1621 gcaattttaa cttggataat tcctagtggt cagtttgaga cagttgttga aaacgtcgca
     1681 ggtatggaaa aaacaacggt tgttccggga acctatcacc aaatcgataa ggcagattat
     1741 ggacaaggtt tttcctctat actaatggca ccagctgaag ggattatttc agctgttgag
     1801 gttgttgctt ttgttctcat cgttggtggt gccttcggga ttatcctaaa aacgggagcc
     1861 atcgatcgag ggctgtttac cttagcaaaa gttctgggag acaaggggat cttagttatt
     1921 ccgatgtcga tgattctatt cagcttagga ggatcaacct ttggcatgag cgaggaagtc
     1981 attcccttat ttgcggtgtt tatttctctg atgttttctc ttggctatga ttccatgact
     2041 gcgatcttga ttctattttt aagtacacaa gcgggatatg ttgggtcgac gattaatccc
     2101 ttcaacgttt taatcgcaca gggagtagct ggcgtttacg gaaatcccct gctttggttt
     2161 cggatgctat cctggattgt cattactggg atttcaattg ttttcaccat gctttatgct
     2221 agcagagtca aaaagaatcc actgtcctct attgtttatg agagtgattt aaaggttaag
     2281 cataagtttc aaaattttga cactgaaaaa gagttttcaa cacaagataa actaattatc
     2341 gccggtttta tcgtaggttt aggaatcatg atttggggta tcatttccaa gggatggtat
     2401 atggttgaaa tttctgcact attcttagga ttaggtcttt ttgctggggg catcgctaga
     2461 ttttctcaaa aggaaattgc tgaagctttt gtcgaaggct gttcagattt cgcctatgct
     2521 gctgtaatca ttgggctagc tagaggagtc ttggtaatct tggaaggtgg aatgattatt
     2581 gacactattc taaacagctt ggctaacctt ttaggtggtc ttccaaaatt cctgttcact
     2641 acgattctac tcatttctca agtaattgtt tccttttttg ttccatcctc ctcaggagct
     2701 gcggcattaa cgatgccggt tatggcacca ttgggtgatc taatgaatgt aagtcgtgat
     2761 gttgttgttc taagtaacca gtttggaaat ggtttaatga atattatctc cccaactagt
     2821 ggtgtattac tggcaggatt agccatcgca gagattaatt tctctaaatg gtttaaggtt
     2881 ggcttcaaaa tatttgccat cttaaccctt gtttctgctg ttttgttata cattgctaca
     2941 ctcttttagt cttaatcatc cattaatagg aggcaatagt tatgattaaa ataaatcaat
     3001 tagcaggaat gaatatccac tatttatttt attctctcaa agaatttttc gcttatcaac
     3061 agaaaattgg tatttcttct gttgaacttt ggggaggtgc tccgcatttt ttcatagatg
     3121 ctttta
//
LOCUS       DAZUMI010000197         3125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000197 DAZUMI010000000
VERSION     DAZUMI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.205"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1721
                     /gene="alsS"
                     /locus_tag="ACG81C_002871"
     CDS             57..1721
                     /gene="alsS"
                     /locus_tag="ACG81C_002871"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5065969.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1734..2444
                     /gene="budA"
                     /locus_tag="ACG81C_002872"
     CDS             1734..2444
                     /gene="budA"
                     /locus_tag="ACG81C_002872"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5065970.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2574..3071
                     /locus_tag="ACG81C_002873"
     CDS             2574..3071
                     /locus_tag="ACG81C_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5065971.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1057 a          515 c          700 g          853 t
ORIGIN      
        1 tttgtgatta tttctaacta ataattaaaa ataaagcata aaaaggacga tgttcaatgg
       61 acgaaaaaat gaaacaaggt tcgaaatctg tagtagaaag tttgattaat catcatgttg
      121 attatgtatt cggtatacca ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc
      181 aaggtcctga attaatcgtg actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa
      241 tcggacgtat tacaggagag ccaggagtag ttatcgcaac aagtggtcct ggtgctagta
      301 acttagctac aggtttggta acagcgacag cagaaggtga cccagtttta gcaattgccg
      361 gacaagtaaa acgaagcgat ctattgaagt tgacccatca aagcatggat aatgcagcac
      421 tgttccagcc aatcacaaaa tacagtgcag aaatccaaga tccagaaaca atttcagaag
      481 tgattgccaa tgcttatcgt atggctaaga gttcaaaaaa aggcgcgagc tttatcagta
      541 tcccgcaaga tgtagtggat gcacctgtcc aagggaatgt catcaagcca ttgagcgatc
      601 ctaaattagg ttctgcttct gcagatgata tccgttacct agctgaacgc attagagaag
      661 caaaactacc ggtactatta gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca
      721 tcaggcaatt agttggaaag acagcattgc ctgttgtaga gacattccaa gcagctggag
      781 tgatctcacg tgaattagaa gcgcatttct ttggtcgtgt gggtttattt agaaaccaac
      841 caggagatat gcttttgaaa cgaagtgatc tagttattgc tattggctat gatccaatcg
      901 aatatgaagc aagaaactgg aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag
      961 cgccagctga aatcgatcct tttatgcagc ctgaaagaga attgatcggt gatatctcag
     1021 ccactcttga tttattgaca ggttcattag agcctcaaca agtgtcagaa gatgcaaaag
     1081 aatatttagc atctcttcaa gcagaattaa cggagcggga cattgttcaa tcaaaaggag
     1141 aagcaggtat cttgcatcct ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg
     1201 atatgactgt taccgtagat gtaggtagtc attatatttg gatggcacgc cactttagaa
     1261 gttatgaacc tcgtcatttg ttattcagta atggtatgca aacgcttggt gttgcattgc
     1321 catgggcgat ttctgctgca ttggtgcgtc caaatacaca aatcgtatca gtttccggcg
     1381 atgggggatt tttattctct gcccaggatt tagaaacggc ggtacgtaaa aaattgaata
     1441 tcgttcattt gatttggaat gatggacact acaatatggt agagttccaa gaaaaaatga
     1501 aatatcagcg ggcgtcaggt gtagactttg gtccagttga ttttgtgaaa tatgcagaag
     1561 cttttggcgc taaaggaatc cgagctacaa gtgtagagga attagaaaaa gctttagaag
     1621 aaggatttgc gacagaagga cccgtaatta ttgatattcc gattgattac cgtgataatg
     1681 agaaattggg agaaacaatc ttaccagatc aattctatta aggaagtgtc gacatgactg
     1741 aaaaaatctt ataccagcac gggacattag gagctttgat ggctggcttg atggatggaa
     1801 cagaaacgat tgctcatata ttagaaaaag gaactctggg attgggaaca ttgcatgggc
     1861 ttgatggtga agtcatcttt ttagatggcg tagcttatca aggaagatca gatggctcag
     1921 ttgcccaatt agatggaagt gaacttaccc cctatgcagc aattacagat tttacgccag
     1981 atactagttt ttctgtctca aaaactgcag atggagaaca attgaaaaaa gacatattag
     2041 taagagaagc cggggaaaac ctatttttgg cagtgaagat aactggatta ttcaaaaata
     2101 tgcacatccg tatcatgccg aagcaagaaa aaccttacag aagactagca aaaatttcag
     2161 agtctcaacc agaattccaa caaagcaatg tccaaggaac attagtcggg ttctttacgc
     2221 ctgaattatt ccaaggagtt gctgcagcgg gatttcacct gcattttatc gatgatacac
     2281 atacatttgg cggacatgta atggattttg aagtggctga aggaaaagtt gagattagcc
     2341 gcatctcgtc ccttgtacag cattttccag ttgatgatcc gacttttgtt cacgaaacga
     2401 ttgattatgc tgatttagca gcagaaatcc aacaagcaga atagaaaaag acagcgagat
     2461 gcgttcaagg tgattaacct gacgtatctc gtttttttga ggagaaaacc tagcgtttac
     2521 aaaaaaactg agctataatc ttctccatag atcaaaaagg agcggttttt atcatgtgga
     2581 cagaaaaaca gaaaaaagat gcatacgaaa ttttattatt acagcaacaa ggattattag
     2641 aagcagaaga taactggtta gcaaatttag caaattcatc tgcattgttg aatgagactt
     2701 tgcctgagac tgtctttgca ggatattatt tattcgatgg taatgaattg attttaggac
     2761 cgttccaagg gcgagtttct tgcacacgta tcaagatggg aaaaggagtt tgtggagaat
     2821 cagctgaaaa acaagaaaca ttgatcgttg atgatgtcaa aacccatgca aattacattt
     2881 cctgcgattc agccgcacaa tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa
     2941 tcggtgtttt agatatcgat tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat
     3001 ttctagaaaa atatgtagat cttttattag caaattttaa tataaatgct ttttcattta
     3061 ttaataacta aaatcgtcta ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc
     3121 ggaaa
//
LOCUS       DAZUMI010000198         3089 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000198 DAZUMI010000000
VERSION     DAZUMI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.206"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG81C_002874"
     CDS             complement(159..1469)
                     /locus_tag="ACG81C_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5065972.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACG81C_002875"
     CDS             complement(1481..2899)
                     /locus_tag="ACG81C_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5065973.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          984 a          631 c          471 g         1003 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattc
//
LOCUS       DAZUMI010000199         3025 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000199 DAZUMI010000000
VERSION     DAZUMI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.207"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            757..>3025
                     /gene="esp"
                     /locus_tag="ACG81C_002876"
     CDS             757..>3025
                     /gene="esp"
                     /locus_tag="ACG81C_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS5065974.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1105 a          439 c          588 g          893 t
ORIGIN      
        1 atgaatttat ggattatcaa aaattggatc agttaattga aagagaaagc tgggaagatt
       61 attacgtggg tctaactttt tcagaaaagt taaacaacta caagttaata gattataagt
      121 atttgagtga aagcaattaa ttattcatgt agagtactag acactttaaa tatggacaat
      181 tcaattataa agaaaaagaa tcaataattt aatgaggctg tgacaaatgt cattttttgt
      241 ttcgctaaaa acgagtgaaa aaatgttgat aactccaatc attcgtttaa gattctagga
      301 atcattcagg aatgctgtga aaccgatact ttataattat aaagaaggtg atttcaaatt
      361 ccgaagaagg aatttgaagt caccttttta cttatgtcac agtctcattc agtaatgcgg
      421 gataaggctc cttattccgc tatttgaata ctctgaagtt taatctaaga aagagtaata
      481 ttttgaaaac atgcttcatc gcatagaaat gaacttaatt tcagaaagaa tacatacata
      541 tctttttata tgttttcagc tgctgaatgg ttaaaaagca caaatatccg aaaaaaacat
      601 gaagaaatgt actttttttg ttgtaatgtg catgaaattc tttaagtaaa ctagttaaca
      661 taagtttagt aagattccta ttctgtgaat aatacgtatc agatttatct tttattctta
      721 ctaagaaaat aactattaag ggagttgatt tgaataatgg ttagcaagaa taataagaga
      781 gtatttctag aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa attaagtgtt
      841 ggggttgcat ctgttttggt tggtgttggt cttgtcttgg gaacaactga actagttcaa
      901 gcacaagatg aaatttctcc aagcacccca cttgaaacag cgataagctc cgtacaagta
      961 ggtgacaaag tagcttcggg gaatactttt caggagaatc ctggttatac gaaaaattat
     1021 aacttttcgg atttacaatt tagtcctcaa gaactaactg gagacacact aaaaggcaat
     1081 acaattggat ttgaagttta cggaaaacat aatattgcag catcaactaa aaactgggaa
     1141 atccgtcttc aattagatga acgattagct aaatatgtag aaaaaattca ggtcgatcca
     1201 aagaaaggaa ttggatcttc tagacggacc tttgtaagaa ttaatgattc gctgggtaga
     1261 cctacaaata tttggaaggt caattatatt cgtgcaagtg atggattatt tgctggggct
     1321 gaaacaactg atacacaaac tgctcctaat ggggtaatta cgtttgaaaa gagcttagat
     1381 gaaattttta aagagatagg catagacaat ctcaagaccg atcgtttaat gtatcgaatc
     1441 tacttggtaa gtcatcaaga cgatgataaa attgtaccag gtattgatag tacgggctac
     1501 tttttaacag attcagatga tttctataat agcttagatg tatctgaaaa taatcctgac
     1561 cagtttaaac atggatcagt aagtgcaaaa tatgaggaac ctaatactca aacaaaagat
     1621 ggttcaggat ccacaggtgc taatggagct ataatcttag atcataagtt aacgaaaaat
     1681 tataatttct catattcagc ttccgcaaag ggcacgcctt ggtatgctaa ttacaaaatt
     1741 gatgaacgat tagttccata tgtagcgggt atacagatgc acatggttca ggctgacaaa
     1801 gtaacatatg atgtttcttt tgaatcaggg aaaaaagtgg ctgatttagc aattgaacgg
     1861 cgcaaagatc atgaaaatta tggtgtaggt tcaattactg ataatgatct taccaaactt
     1921 attgattttg ctaatgcaag tccacgtcca gtcgttattc gatatgtttt acaattgact
     1981 aagccattag atgaaatctt agaagatatg aaagcaacag cacaagttga agaaaataaa
     2041 ccatttggtg aagatttcat ctttgattct tggttgtcgg atacgaataa aaaattaatc
     2101 cagaacactt atggaacagg ttattattat ttgcaagata ttgatggtga tggaaaccct
     2161 gacgataaag aagagagcgg agacacgaat ccatatatcg ggaaacctga attagaagaa
     2221 gtatatgatg ttgacacaac agttaagggg aaagtattca tccacgagtt agcgggaaca
     2281 ggtcacaaag cccaacttgt tgataaagaa ggtactgtat tagcagaaaa aactatcgct
     2341 ccaaatgaaa aagatggggc tccaatttca gatactgtag aatttgaatt tacgggtgta
     2401 gattcaagta aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc tccaggtttt
     2461 gataaaccag aagaaggttc aaccgttatt aaggaatcac caaaagcggt tgataaacaa
     2521 accgtggtag ttggatttaa accagatgct aaagaatcaa ttcggaataa taaaaactta
     2581 cctgaagatg cagagtattc atggaaaaca gagcctgata cttctaacgt tactgatagt
     2641 acgaaaggta ttgtaactgt taagatcgga aatcgaactt tcgacgtgga tgtagagttt
     2701 gctgtaaaag cttctcaagc tatggaaaat gatgcaacat acgtacctat aacaacaact
     2761 ccagaaacga cagttcaaag tggaaaacct acatttgata aaccagatgt tcctttagct
     2821 aaagatgcct tttcaatttt agatgtctat aataaggact tcggtaatgc aagtgttgat
     2881 gcaaatactg gtattgttac attcactcca gctaaaggtg taggagaatc ggagccgatt
     2941 actggaacaa ttcctattaa aattgtttac caagatggtt ctgtaggcac gaccgattta
     3001 gcagtaactg taagtaaaga tattt
//
LOCUS       DAZUMI010000200         2962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000200 DAZUMI010000000
VERSION     DAZUMI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.208"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(182..796)
                     /locus_tag="ACG81C_002877"
     CDS             complement(182..796)
                     /locus_tag="ACG81C_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5065975.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1402..1779)
                     /locus_tag="ACG81C_002878"
     CDS             complement(1402..1779)
                     /locus_tag="ACG81C_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065976.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2079..2960)
                     /gene="galU"
                     /locus_tag="ACG81C_002879"
     CDS             complement(2079..2960)
                     /gene="galU"
                     /locus_tag="ACG81C_002879"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5065977.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          898 a          620 c          464 g          980 t
ORIGIN      
        1 tataaaaata acaaacttta aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa
       61 taactaaaac aaaaaaagta gtcgttgtta atcctcatta ataatcgttt tctttttttc
      121 aaacgctcca tttttatgtg gaacattatt tttttaagga actttgtcaa catgtggata
      181 actattttcc acttgtgtcc tggactagct gttttgctgt aaaatacaaa acttgctgca
      241 gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc tggatctaga tttggcaaag
      301 tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca aattcaaata
      361 atcgcaaacc actgctatgt tgcgatttga tacttcgtga caaatcaaat ccagtaacaa
      421 tgattaatgg atgaccattt tctttgatcc actgagctag ttcgatgatt gccgggtttc
      481 ttccttcatt cgaaagaaga agaaaaatgt cttctgattt gacagaaagc tgatcaataa
      541 ttgcctgtcc tgcaccttct agcgtttcat agacacctcc ggctggattt tcaatcaata
      601 tacttggtac aagatccggg gtttgatccc aaaattcaaa tgctacacca cttagtgtgc
      661 ggctagcata tatctgcaat ctaccacctt gattaatttg tttcgcgatc caagtgccag
      721 cttgaagcat cagcggctct tctatcctta atttctcttc caattggtga taatagtcaa
      781 aataacataa actcatttat ctctcccgcc tagctatact tgacatgaat agaaacagct
      841 gcctaatcta aggaagtaca taaaaaaaca gtagttagac agtaacacat gtggtctttg
      901 ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca ataccttgac atagcaaata
      961 aaacaacgtg cagaccatgg aaaatatcta cataggggta gaattttcac cctagattag
     1021 gtacaataaa ttaggtcgat gcactgcatt gactatggca aaatgatcgg tcagggtgta
     1081 tgtgtgtgac tggtcattcc ctgattctta gcgccaacta agggtcaggg agcctttttt
     1141 tattcagctg tttctatctg aatttattgt aagtaatctc ttatccaagt gcaagcaaga
     1201 aaatacaaaa aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg
     1261 cttatcccga tacaacaagc attcctttaa aaaagattct tttatataag tgaaaaaagc
     1321 ttttttccac caattaaata aatctctcat ctcaatagtt tatccattat tttatctttt
     1381 tttgcgtata cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga
     1441 atctgtacta gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt
     1501 catatatggg atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc
     1561 ttcccaacta cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata
     1621 ccagataaaa atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact
     1681 aataagcaat agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat
     1741 atcaagcagc gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt
     1801 ataattaaaa aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct
     1861 cttatgtaat cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt
     1921 ttctgaatct attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct
     1981 agagtcaaaa aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac
     2041 aaatagcctt tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt
     2101 ttactcaact ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca
     2161 tattcaatat ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta
     2221 aactctctcg catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt
     2281 atctcgtttc ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt
     2341 agataacgtc cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca
     2401 aaccctttta ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat
     2461 ttagatgttt cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca
     2521 taatccatca tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc
     2581 atgacgacaa acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt
     2641 cctcttggat gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa
     2701 gcgagtaatt ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca
     2761 aagtgatctt caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc
     2821 gaatccaatg cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt
     2881 tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg
     2941 actgcttttt tgaccttcac tt
//
LOCUS       DAZUMI010000201         2953 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000201 DAZUMI010000000
VERSION     DAZUMI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.209"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..406
                     /locus_tag="ACG81C_002880"
     CDS             89..406
                     /locus_tag="ACG81C_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5065978.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="ACG81C_002881"
     CDS             complement(497..862)
                     /locus_tag="ACG81C_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065979.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="ACG81C_002882"
     CDS             1070..1552
                     /locus_tag="ACG81C_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065980.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="ACG81C_002883"
     CDS             1749..2639
                     /locus_tag="ACG81C_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS5065981.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tgt
//
LOCUS       DAZUMI010000202         2914 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000202 DAZUMI010000000
VERSION     DAZUMI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.210"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG81C_002884"
     CDS             <1..227
                     /locus_tag="ACG81C_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065982.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG81C_002885"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG81C_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG81C_002886"
     CDS             1635..1841
                     /locus_tag="ACG81C_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065983.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG81C_002887"
     CDS             1862..2458
                     /locus_tag="ACG81C_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFS5065984.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1059 a          396 c          575 g          884 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttt
//
LOCUS       DAZUMI010000203         2892 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000203 DAZUMI010000000
VERSION     DAZUMI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.211"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(244..1449)
                     /locus_tag="ACG81C_002888"
     CDS             complement(244..1449)
                     /locus_tag="ACG81C_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5065985.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2143..2529
                     /locus_tag="ACG81C_002889"
     CDS             2143..2529
                     /locus_tag="ACG81C_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5065986.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            2627..2818
                     /locus_tag="ACG81C_002890"
     CDS             2627..2818
                     /locus_tag="ACG81C_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5065987.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         1050 a          467 c          362 g         1013 t
ORIGIN      
        1 cttaaaattg ctaccattaa tatgactgat agattctcta gcttcttcac ataaactttt
       61 tactttatta attactactt tgtcatattc atttaacaca ctattattac cattacttag
      121 acgctcagat tctacgaaac aactcaaatc agaaatttcc caaaaaattt tttgttaaaa
      181 tccaatttcc tcaactcctc caaactacgt aaatgatata gtctcataat aaaataaaac
      241 ctctcagcta atgttgttga aattatccac taaacctaat tgatattgct taggagatac
      301 acctgcgtac tttttgaata ctctcgaaaa gtgtgaagaa tctgaaaatc ctaaacctaa
      361 agaaacttca ctgatagtat ggtttgattt caacattatt ttagccgtag aaatttttcg
      421 cttagagata taattataaa tcgtaatatg catttcattc ttaaagcttt ctcttaattt
      481 agtttcactt acagaaaaat aattagctat ttcctttaca gtaattcttt tatagatatt
      541 taagccgatg aattgaacga cagaagaaat taagggagaa agatgctcta tcttagtttt
      601 tccgatttcc ttagtataat aaacaatccc agaatccctc acttttaaga cctcaggtaa
      661 cgatacagct aattcgtttt cttgtatcaa agcatctcta ctttgatacg cttctattat
      721 atccattcct aatgttatag acgtttgtgc taacttttca aaaacaatga tagaataatt
      781 tttttcagac cgaatcgtat ctccactaac acttggaatt attccattac tcagattaaa
      841 aaccatgttt tctaaatttc gaatatctcc atttttaaca tattccagta ttttattttc
      901 ataaagaaac agatatattt ctggatcgta attttgtgat aacagattat ctattttgat
      961 ttgctcgata gttttactta gatttcctgt atattcaatt aacggttcat ggaatagatc
     1021 ttctattttt ccagtaaaaa aataatgaac taaaatcaga atatctcgaa tgtctcctaa
     1081 agaatacaaa gtcaaatttt gcaatgaatt atagagtcgt tctctatcag ccattgcaac
     1141 attcaaattg ttcattttta gtttaaaaaa atctttatca atattatttg ctctaaatgg
     1201 accaaataag taatatctgg ttttaaacat gtatagaaga aacatctcat taaaatcacc
     1261 agtaaagaaa tggaaatctt tagttttttt aatattatca gttagtaact ttttataatc
     1321 ataaaaaaaa taagtagttc tatctgagcg atactctttt ataatactga attcggaatt
     1381 aaaaaccacg ataggtaact ttaaacaaac atgaagagac tttaataaga cttctttatc
     1441 tgaaaacact tttgtttctc tctttccttt ctacatcatt aaatagtatt ttatacgtta
     1501 tctaaaatac tattcctctg tcaaaataag ctaacttact tcattataaa atatccactt
     1561 atcgctttca tgtatattcc accgtaaaaa gtacatttta tcaccgagaa ctcatcattt
     1621 ttcatcatga tgaatactat ttacatatat ctagttcaaa gacaataatt attatgatcc
     1681 gttattatct aagcactata cagataatag gaatttttcg tttctaaata ctccaatttg
     1741 ggaatccaat aaaaattttc gttcaaatac gtgtctattc gaactctttt ctagttgtta
     1801 attatgaagg gggaagaaat aaaaaataca atctatacac gaataagaat attatccaaa
     1861 aggaggtttt agaaaagatt tattatacgt aaaaagctca agacattttc ttagccataa
     1921 ggacatgttt gatatttaag aagatatatg aattttcttt tctaagtata aagataacta
     1981 tccattatca actaaaaaaa catttttgaa aataaacatc agcagtccta tcaatgcatg
     2041 tgagcttata gaagttattc gcagcgcagc ataattgcat tagcttcttt tcgtgttttt
     2101 tgcctctttt ccctttaaat cttaatggag gaggtgtgca atatgtcaga taacgacgaa
     2161 cgcgtaaatg cccagttgat tcgattcttt gaaaaggtca ttttgaacac cgctaatact
     2221 tattataaaa aacaagaaga aattcaaaaa catgaacaac ttgatcaact cccaccatat
     2281 ttgtttacta aagatactat aaaatttaaa caaccgatta atgttttaaa tgtgactctc
     2341 cttgttgaag atataaaatt agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt
     2401 tttcttatcg aaaaatttat ctttaataaa actgataaag aaattggaaa ggttctgggt
     2461 attacaagac agggtgcgac aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg
     2521 ctggtttaat atctgcataa taactaattt tgtatgcttc tttgctcgtc ctcctccttc
     2581 cccaaaaaat aaatagatta taagttcatg ggaagaaggt aaatttatga atactttaaa
     2641 agaagatttc attttagcaa agaatggaaa tgaagaagca gttgtggcca ttctcaaaag
     2701 atttagcgct ttggtccata agcaatcctg gagaactgga aaatatgatc aagattgtta
     2761 tcaagaatgt atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta aagaataact
     2821 caactaaggg agtctgtgac ataagtaaaa agatagtttc aaatttcgaa caataaattt
     2881 gaagccatct tc
//
LOCUS       DAZUMI010000204         2790 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000204 DAZUMI010000000
VERSION     DAZUMI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.212"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACG81C_002891"
     CDS             382..570
                     /locus_tag="ACG81C_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5065988.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACG81C_002892"
     CDS             648..1661
                     /locus_tag="ACG81C_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5065989.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACG81C_002893"
     CDS             complement(1728..2762)
                     /locus_tag="ACG81C_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5065990.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT          866 a          536 c          544 g          844 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa
//
LOCUS       DAZUMI010000205         2776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000205 DAZUMI010000000
VERSION     DAZUMI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.213"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1438
                     /locus_tag="ACG81C_002894"
     CDS             <1..1438
                     /locus_tag="ACG81C_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS5065991.1"
                     /translation="DSRKSLKMTQQQLAVATGINRALISRLESLDFIPSIQQLESLAE
                     VLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLAQHNQVTAVDILPEK
                     VKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVVIAAPTNYDSKQNYF
                     DTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNILFSPEFLRESKALY
                     DNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFMGFTEAEAVKLFANT
                     YLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQ
                     LLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSWNAEKEKEVVIGVYR
                     LTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFGSKVINNLDDFKNIS
                     HSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1506..2597)
                     /locus_tag="ACG81C_002895"
     CDS             complement(1506..2597)
                     /locus_tag="ACG81C_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5065992.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          960 a          505 c          442 g          869 t
ORIGIN      
        1 tgattctaga aaatctctga aaatgacaca acaacaactt gcagtagcta ctggtatcaa
       61 ccgtgcctta attagtcgat tagaatctct cgattttatc ccgtcaatac aacaattaga
      121 atcattagca gaagttctaa attttgaacc aacagatcta tttataaatg aatcaaataa
      181 atcaaacaga acaaaacatc agtcgaagaa tattgctgta gccggtactg gttacgtagg
      241 tctttccatt gctactcttc ttgcccagca caatcaggta acagcagtag acattcttcc
      301 tgaaaaagta aaacaaatta acaataagaa atctccaata caagacgatt acattgaaaa
      361 atatctagct gaaaaaaatc ttaatttaat tgcaacgact gatggtgcag ccgcctatag
      421 agaagccgat tttgttgtca ttgctgctcc aacaaattat gatagtaaac aaaattattt
      481 tgatacttct gctgtagagt cagtaatcaa attggtgtta gaagtcaatc caaaggcaat
      541 aatggtcatc aagtcgacgg tacccgtagg ctataccgaa agtattcgta aaaaatacaa
      601 tactaaaaat attctattca gcccagaatt tctcagagaa tctaaggcat tatatgacaa
      661 tctatatcct tcgcgaatta ttgtcggcac ggaccaaact gacaaaactt tacgagaagc
      721 ctctcacact tttgcagatt tactccaaga aggagcaatc aaaaaagata tcgaaactct
      781 tttcatggga ttcacagaag ctgaagcagt taaattattc gctaatactt accttgcact
      841 tcgagtatct tacttcaatg agctggatac ttatgcagaa atgaaaggac taaatacaca
      901 agaaatcatt cacggggttt gcttagatcc aagaattggc acacattata ataatccatc
      961 ttttggatat ggaggctatt gtttaccgaa agatacaaaa caacttctca ctaactataa
     1021 cgatgttcct cagaatatga taagtgcaat tgtcgatagt aatcgtacaa gaaaagactt
     1081 tatagcagat cgagtgttag aacttgctgg cacgtatgag gctaacagtt cgtggaatgc
     1141 agaaaaagaa aaagaggtag tcataggtgt ataccgtctt acaatgaaga gtaattcaga
     1201 caacttccgt cagtcatcta ttcaaggagt tatgaagcga attaaagcaa agggggcaac
     1261 tattgtaatt tatgaaccaa cacttgaaaa taacactaca ttttttggtt caaaagtaat
     1321 aaataattta gatgatttta aaaatatcag tcacagtatt attgctaatc gttacgatag
     1381 taatcttgat gatgtaaaag aaaaagtata taccagagat ttatttcaac gagattaaaa
     1441 aataacacct cagatttgta gtgtctgagg tgtttctata aacttttata agtaaaaaaa
     1501 tacaattata cttcataaaa agacttatac cactgcgcaa atttacgaag cccgtcacgc
     1561 aaacttgtac ttggtttaaa ttcaaaatct cgttctaacg cactagtatc tgcataagta
     1621 actggcacat ctcctggttg cattggaact agttcggtat gagattcaaa gtcgtaatct
     1681 tctggtagca cccctgctgc aattagctct tgttgtagaa tatctacaaa atctagtagg
     1741 ttttccggcg tattgttacc aatattataa atatgatatg gtgggattgg tagcccatct
     1801 tccccatcaa ttttctctgg agcgcactgc ataaccctct ttactccttc aacaatatca
     1861 tctatatagg taaagtcacg cttgcaattc ccataattga atatttggat attatcacca
     1921 gcacgaagtt tatcagtaaa accaaaataa gccatatctg gacgcccagc aggaccatat
     1981 actgtaaaga aacgtaaccc tgtagacgga atattgtaaa gtttagaata tgcatgtgcc
     2041 attgattcat tacttttctt agttgctgca tacaacgata cttgattgtc tactttatcc
     2101 tctgtagaat aaggaatttt cttattagta ccataaacgg aagacgatga ggcatacact
     2161 aaatgttcca caccagattc tccattatca tatgaatgac gacaagcttc aagaatatta
     2221 tagaatccta ttatgttaga ttcgatatac atatctggat tagtaattga ataacggact
     2281 cctgcctgag ctgctaaatt caccacgata cttggtttat aattatcaaa gatgaaatca
     2341 attgttgctt tatcagcaat agaacctttg ataaaattcc aagtagatcc tggatggttc
     2401 tttgcctccg tttctatctc tgataatcta tagttcttaa tagagacatc atagtaatca
     2461 ttcatattgt caatgccaat gatttttatt gatgactgag tacgtaataa ctcaagaacc
     2521 aaatttgatc caataaaacc ggctgcgcca gttattaata ttgttctatt tgataaggac
     2581 gtgtttaatt ccagcatttt ttagcactca ctttcttttt ttcttatgaa tacatatttg
     2641 ttatatatta aaaaatctta tttagactta tctattgtca cttttatcat tctaatcttt
     2701 gcacgtagta gaataattat acagatagga aattgctaag caacccactt ctttctatta
     2761 tctattaact tattct
//
LOCUS       DAZUMI010000206         2527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000206 DAZUMI010000000
VERSION     DAZUMI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.215"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACG81C_002896"
     CDS             <1..1368
                     /locus_tag="ACG81C_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065993.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..>2527
                     /locus_tag="ACG81C_002897"
     CDS             1355..>2527
                     /locus_tag="ACG81C_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS5065994.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNF"
BASE COUNT          843 a          378 c          427 g          879 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttc
//
LOCUS       DAZUMI010000207         2491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000207 DAZUMI010000000
VERSION     DAZUMI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.216"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1658)
                     /locus_tag="ACG81C_002898"
     CDS             complement(366..1658)
                     /locus_tag="ACG81C_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5065995.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1932..2192)
                     /locus_tag="ACG81C_002899"
     CDS             complement(1932..2192)
                     /locus_tag="ACG81C_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5065996.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          382 g          800 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg cctataataa gtatgggaat aagattagaa
     2101 aataacaccc cgagccaatc tgtgaaaatt actatattta ctaatatccc tattaacata
     2161 cctatcatat ttattaaatc aatttttttc attagatatt tctcctttat taaatctgaa
     2221 gtacttatat taaaataatt ctaaactatt tacctcaaaa agtgcaataa attatttctg
     2281 aacaataaaa tgatagtcaa aacaaaaaaa tattgttaat cgttcagagc catccgagag
     2341 tgtaaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaaggcgc ttctgtagaa
     2401 taaagttaac gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca
     2461 gaaattgtgc aaactctagt cactaaaggc g
//
LOCUS       DAZUMI010000208         2402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000208 DAZUMI010000000
VERSION     DAZUMI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.217"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG81C_002900"
     CDS             207..2069
                     /locus_tag="ACG81C_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5065997.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="ACG81C_002901"
     CDS             2069..2401
                     /locus_tag="ACG81C_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5065998.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          716 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 at
//
LOCUS       DAZUMI010000209         2365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000209 DAZUMI010000000
VERSION     DAZUMI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.218"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1451
                     /locus_tag="ACG81C_002902"
     CDS             150..1451
                     /locus_tag="ACG81C_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5065999.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="ACG81C_002903"
     CDS             complement(1555..1755)
                     /locus_tag="ACG81C_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5066000.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2151..2300
                     /locus_tag="ACG81C_002904"
     CDS             2151..2300
                     /locus_tag="ACG81C_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066001.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          768 a          454 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataac
//
LOCUS       DAZUMI010000210         2337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000210 DAZUMI010000000
VERSION     DAZUMI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.219"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..>2337)
                     /locus_tag="ACG81C_002905"
     CDS             complement(226..>2337)
                     /locus_tag="ACG81C_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5066002.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT          597 a          522 c          425 g          793 t
ORIGIN      
        1 tgagccagaa tgtggttcat tctgactcag atttgatttt gcacaactca tcagtcctta
       61 ttgacaaaga gccacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
      121 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
      181 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
      241 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
      301 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
      361 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
      421 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
      481 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
      541 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
      601 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
      661 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
      721 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
      781 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
      841 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
      901 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
      961 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
     1021 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
     1081 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
     1141 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
     1201 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
     1261 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
     1321 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
     1381 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
     1441 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
     1501 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
     1561 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
     1621 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
     1681 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
     1741 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
     1801 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
     1861 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
     1921 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
     1981 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
     2041 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
     2101 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
     2161 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
     2221 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
     2281 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaa
//
LOCUS       DAZUMI010000211         2330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000211 DAZUMI010000000
VERSION     DAZUMI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.220"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..167)
                     /locus_tag="ACG81C_002906"
     CDS             complement(<1..167)
                     /locus_tag="ACG81C_002906"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5066003.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALI"
     gene            complement(502..732)
                     /locus_tag="ACG81C_002907"
     CDS             complement(502..732)
                     /locus_tag="ACG81C_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5066004.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(747..1697)
                     /locus_tag="ACG81C_002908"
     CDS             complement(747..1697)
                     /locus_tag="ACG81C_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS5066005.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1694..>2330)
                     /locus_tag="ACG81C_002909"
     CDS             complement(1694..>2330)
                     /locus_tag="ACG81C_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5066006.1"
                     /translation="AIVFGLLASIIITFIKSNSVIKGDTAIGITFSSFLALGVILIGV
                     ANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLITSFDPLMAKAMG
                     MNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATV
                     GACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
BASE COUNT          752 a          435 c          295 g          848 t
ORIGIN      
        1 ttaataagcg cattaatatg cggctgaata atttcttcaa ataacgcttc cttagaagta
       61 aaatgtttat aaaaagcacc cgtagttacg cctgcggtca agcacagttg ccttatctta
      121 gtttgctgaa atcctacttt ttcaaaatgg cttagtccac tttctattaa tttttggtga
      181 gttgtaatat aatctctggt ttctatgggt acttcctcct aataagatga attaactata
      241 ttatcttaca caggacttga agtttcaatc gttaacgtct attactgaac tcattcctta
      301 gagctaaact aatgagaaag agcaaaataa ctgatacaaa aagcaatcct acaagaatta
      361 atagactcat ctaaatcatt caccttttct aaaataatcc gagaaagatt taaaaaaatt
      421 atatccagct attaggataa gtcctatcat aatcactcca atagccattt tcttatcctc
      481 cctaaaaata aagcctttta cttaaaacct ttttttgtaa tcgtcaataa attttttccc
      541 tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt tagtcagcat
      601 ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat tttttaattt
      661 cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag tagttgtttt
      721 aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg
      781 ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca
      841 gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta
      901 tcgtccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact
      961 aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca
     1021 gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt
     1081 tttttatcat tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat
     1141 ttttcaatat acttatctag attttcttta tagaattttt tattatcagg atctttttcc
     1201 gctaactgtt tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca
     1261 tggggatctt ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca
     1321 cttgctgcaa aatagtcttt gttttcctct ttgttcgcat tttttactaa ttttgtaaac
     1381 caagcatttc ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa
     1441 gttttttgaa catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg
     1501 atactgtgta aatcaatttt atcttttgct atattttcag taatatctgc taaaatcgaa
     1561 ttagtaacta ctacctttaa tttttcgttc gaagccactg tatctttgtc tttactattt
     1621 gttgaacatg ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct
     1681 ttccattttc tcatcataac tttctctcct tctttcttaa aaatttttgt tttggtgaga
     1741 ttagaaaact aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta
     1801 tgttaaaact ataaccaatg aacaaaccta gcaatgatga gcacgctccg actgtcgccg
     1861 aaataaatat catagttttt aaactgtttg catataaata tgctgtagca gctggtgtaa
     1921 ttaacattgc aacaattaat atagttccca cactttgcat agctgttact gctactaaag
     1981 ttaatagaat cattaataga taatgataaa aactgacatt catccccatt gcttttgcca
     2041 ttaatggatc aaaagaagtg attaataatt ctttaaaaaa tagaacaact gtaaccaaca
     2101 ctaaaacaga aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc
     2161 caaataagat atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta
     2221 atgctaaaaa agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc
     2281 tttttataaa agtaataatg atagaagcta atagaccaaa tacaatcgca
//
LOCUS       DAZUMI010000212         2242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000212 DAZUMI010000000
VERSION     DAZUMI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.221"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..1032
                     /gene="msrA"
                     /locus_tag="ACG81C_002910"
     CDS             199..1032
                     /gene="msrA"
                     /locus_tag="ACG81C_002910"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5066007.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1112..2101
                     /locus_tag="ACG81C_002911"
     CDS             1112..2101
                     /locus_tag="ACG81C_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5066008.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          819 a          366 c          445 g          612 t
ORIGIN      
        1 aaaatagttt tccataaaag gtaaattaac ttttcattta taaaataaat tgattaaaaa
       61 ggagaatctg tcgataagga ttctctcttt tttatacgaa aaatctgtag ggggatgaag
      121 taatcgctta tttcctcact ttatgataaa ttacagctaa agtcactagc ttgtcttctt
      181 cggaaaggag gagaatctat ggatgaggaa caaatattca ttcaaacact ttataatctg
      241 attttgaatc cgaatacacg agattgggaa cgaaaagtat tgattcagac aaagaatgac
      301 ataagagaaa atatatccgt taaagaacag ttatcaaaac tagaagcaac actacgtcca
      361 ttagctatcc gaatgaatct gacgcctgat gtaatggact tttatttatt gattacggaa
      421 ggatttgata aggagcaaaa atatgacttt tccaagcatg ccatgcaaga tgctgactat
      481 caagaacgtg cagtatttgc agggggatgt ttctggtgta tggtcgagcc gtttgaatcg
      541 aaaaaaggga tcctatctgt tttgtcagga tacacgggcg gacatgttga aaaaccaaat
      601 tatgatcaag taagtggtgg ttacacaggt catgtggaag ctgtggagat catttatgac
      661 acaagagaaa taagttatag cgagttgctg acaatctatt ggcaaattac tgatcccaca
      721 gatacatttg gacaatttca agatagaggg aagcaatatc gtccagtgat tttttatcaa
      781 ggcgaacgac aaaaagaatt agcggaacaa tcaaaacaga aactagacag ttcaggaact
      841 ttccaccaac caatcgtcac aaaaatcgaa ccagccggta cgttttggcc agcagaaaac
      901 tatcatcagc aattttacaa aaaacaaccg aaacgttata aaaaaatcca gcaagcacgt
      961 aatcaatttc tgatctatca acgagtaaaa aacaaatggc aaaaaaatat acgaaaaaat
     1021 cattttgatt aactcctttt ctttaaaatg tagtaaacta aaaatagaaa aaagaaagcg
     1081 ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg cacgttatga
     1141 aaaaatggaa tatcgtaaat ccggtcattc aggtttgaaa ttaccgattt tatctctcgg
     1201 attatggcaa aattttggtg attatgatcc tatccataat caaagagaga ttttaagagg
     1261 cgcattcgac atgggaatta cccatttcga ccttgcgaat aattatggag gccctgctgg
     1321 agctgcagag aaaaactttg ggcgtatttt tagagaagat tttcaagcct accgtgatga
     1381 actgattatt tctagcaaag caggttatca tatgtgggaa gggccatatg gggaatgggg
     1441 ctctcgtaaa tccatcattt caagttgtga ccaaagtctc caaagaatgg ggctagatta
     1501 tgttgacatc ttctatcatc atcgcccaga ccctgataca cctttggaag aaacagcaga
     1561 agctttgatg caacttgtaa gacaaggaaa agcattatat attggtatat ccaactataa
     1621 tggagaagat acaaaaaaaa tgactgaaat tttaaaaaga aaggaagcac cgttcattat
     1681 tcatcagatg cgctacaata tgttttctcg tgcactacta gaagacgatc tatctccagt
     1741 attagaagaa gaagggctag gggcaatcac gttcagtccg cttgctcaag ggttgctgac
     1801 aaatcgttat ctacatggta ttcctgaaga ttctagagca catcgtaagg aaataccatt
     1861 cctttcagaa gaacaagttg gatcaactct ggaaaaaatc aaagcactgc aaacaatcgc
     1921 cgtatcacga ggacaatcat tagcgcaaat ggcccttgca tggaatttgc gacaaaaatc
     1981 tgttacaagt gtactggtcg gagcaagtcg gttaagtcag ttacaagaaa gtgttcgaat
     2041 gatggataat ttagatttct caccagaaga agaattgaga atcgaccaaa ttctagaata
     2101 gaaacgatgt aatcaaactt tttatccata tattaaaaaa aacaaaagat gacatcaaga
     2161 atcgaatgaa gatcgtctgt tattgaacat catatttttt gtcgttcaaa ataaaaatga
     2221 tcgaaggcta ggagtgaatt tt
//
LOCUS       DAZUMI010000213         2096 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000213 DAZUMI010000000
VERSION     DAZUMI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.222"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG81C_002912"
     CDS             <1..375
                     /locus_tag="ACG81C_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFS5066009.1"
                     /translation="TQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKIKK
                     MLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQFIA
                     ADYDADLADQFINGNNFMPTTN"
     gene            complement(521..997)
                     /locus_tag="ACG81C_002913"
     CDS             complement(521..997)
                     /locus_tag="ACG81C_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5066010.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(957..1313)
                     /locus_tag="ACG81C_002914"
     CDS             complement(957..1313)
                     /locus_tag="ACG81C_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066011.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1291..1476)
                     /locus_tag="ACG81C_002915"
     CDS             complement(1291..1476)
                     /locus_tag="ACG81C_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066012.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1477..1749)
                     /locus_tag="ACG81C_002916"
     CDS             complement(1477..1749)
                     /locus_tag="ACG81C_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFS5066013.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1746..1946)
                     /locus_tag="ACG81C_002917"
     CDS             complement(1746..1946)
                     /locus_tag="ACG81C_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066014.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT          635 a          393 c          297 g          771 t
ORIGIN      
        1 actcaagcca cacaagctaa atctgagcaa ttggaacaaa ttgctggagt tggttataga
       61 aaagctctag aatttctaat taaagattat gcaatatcta aaaattcaga ggatgaagat
      121 aaaataaaaa aaatgttatt aggtaaaaca attgaaaatt atttgaatga ttttcctaaa
      181 ctccaaagat tagccaaagc tgctacttgg attggtaatg atgaaacgca ctatgttcgt
      241 agacatacta ataaagatgt tcatgatatg gatgcattta ttaaatcagc agcgcaattt
      301 atcgcggctg attatgatgc tgatttagct gatcaattta taaatggaaa taactttatg
      361 ccaactacta attaaaagaa taaaagagcc acagatacca attaataaaa cggttgggat
      421 atcttggctc tttcatattt ttttgacact aatagaatat catgctgcta acaatgttta
      481 catagctaca atgaccgaac atttagtgaa cagtgtaatt ttagaataaa gagagttgtt
      541 ctttgtactt ctcgctattg taaaaatcaa tttcgttttc ttttttcaat cggtcagctt
      601 gtctgtgttc atactcatct aaaaagttca atgtctttct aatttctgca tgtcgctgtc
      661 tgacatacga tgagctatac ccaacagatt cagcaatatc ttctaagctc atttgatcaa
      721 catatttcat cttaacaatt tgattatcga taccagaaaa gctatctatc agcaataaca
      781 tttcttcttt ttgctcgatc aacaattcca attcattttc tatcttttgg atattctctt
      841 ctaaagaaga agttctcgaa ttcttttcga tgcggacgtt tgctaaatca ccacagaccc
      901 atcgattcaa ttcaagctta cttttattaa gattccactt taagtaaaga atctgctcat
      961 ctagttcttg gtaatctttt aaccaatgaa atctcacaaa cgccacccct tatggtagaa
     1021 tagtattgcg ctgctatctc gaaaggggta gcttttttat tttttcttag ccgttccatt
     1081 ctggattgcc ttttgttcca aacgtcgttt tttcttctta atctttgatt ttgtttttcc
     1141 catacaattc tcctttccga aaacacattt tgttcgattt ttaatactaa ataaaagttg
     1201 gccagaattt ttcgttcgtc tttactcatc tttttttgaa taagattact agcataaaaa
     1261 tagttagtac cacgaaaatg aacaggtaaa tcaattacgt tcaccttctt catgccagtt
     1321 atcagccact agaatctttg cgttagtgcc gccaccgttt tcttttttgt atttttcaaa
     1381 tgctttataa gcagctgttt ttgatttata gatttttccg gaaccgactg ggaacatcca
     1441 tgtgccctct gtgatagcta agatgtatac tttcatttaa ttcacccctt taatccccaa
     1501 cactacatat ccggacttct gcatgtaatc agtaatataa gtgatctctg ctttgtatga
     1561 gtcaccagtg taactaaatg ttggtctcgt tagtccaggt aattcatgtt taatgtattt
     1621 tttatattca cgtagtatta actgatcacc aactttgaag tcacgatcat tcttgcggat
     1681 ctcgaattgt ttgcgtccgc tcgtcactgc ttcaaaatat tctggtagta tttttagttc
     1741 gtggatcatt ctgtttcctc cttcaaaatt cgctcatcat caaaaagtga atctccacaa
     1801 aatccttctc ccaaatattt atcgttcatg tataagtcaa ccaaatattt gtgatctgct
     1861 tctcgatatt cattaatatt aacgatcttt attttgtaaa cattaccttc gtcgctcact
     1921 atcttgaatt gttctctgac tttcaccacc gttcctcctc tttttgaaaa aattttagtg
     1981 tttttgtaag accccctaaa tcagcttttt tatttgatat aatacttgta tcgattaatc
     2041 aaatcgaaat taaatttaaa aaggtgattt atcatgaata tcaacaattt agtaaa
//
LOCUS       DAZUMI010000214         2034 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000214 DAZUMI010000000
VERSION     DAZUMI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.223"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            649..981
                     /gene="traE"
                     /locus_tag="ACG81C_002918"
     CDS             649..981
                     /gene="traE"
                     /locus_tag="ACG81C_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5066015.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1600..1941
                     /locus_tag="ACG81C_002919"
     CDS             1600..1941
                     /locus_tag="ACG81C_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS5066016.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          709 a          271 c          429 g          625 t
ORIGIN      
        1 gaaataatta atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga
       61 gttataataa tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata
      121 ttacttcttt tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg
      181 tctgctatgc ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca
      241 attgaaaata caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag
      301 cgttcataca cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat
      361 tacccgcaat ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg
      421 ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa
      481 tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga
      541 tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa
      601 caagaaaaac ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca
      661 ggaattttga gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa
      721 atcacgaaga aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata
      781 atccagaaag acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata
      841 attttaaaag atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac
      901 caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg
      961 atggggcgtt tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc
     1021 ttgcttgaaa atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag
     1081 tagacttgat ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt
     1141 aacgtaaaaa attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt
     1201 gtgtaaataa taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata
     1261 gcgtctagat taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga
     1321 ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca
     1381 gtacatgtag atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc
     1441 tactagggtt tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag
     1501 taaacgaaaa acgataaaat atgaacaagt cctgaataaa gtgaaaaaat tgatataaaa
     1561 catagagaaa atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat
     1621 atacgattgg gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt
     1681 gtgacgttta gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     1741 gatttatacg atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat
     1801 gatgaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     1861 gttgaagata cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt
     1921 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     1981 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaat
//
LOCUS       DAZUMI010000215         1979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000215 DAZUMI010000000
VERSION     DAZUMI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.224"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACG81C_002920"
     CDS             <1..367
                     /locus_tag="ACG81C_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS5066017.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACG81C_002921"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACG81C_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1979
                     /locus_tag="ACG81C_002922"
     CDS             1330..>1979
                     /locus_tag="ACG81C_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5066018.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DAZUMI010000216         1966 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000216 DAZUMI010000000
VERSION     DAZUMI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.225"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            691..1917
                     /locus_tag="ACG81C_002923"
     CDS             691..1917
                     /locus_tag="ACG81C_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5066019.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT          715 a          283 c          405 g          563 t
ORIGIN      
        1 tttagatttt ttggctaagt aaatgcattt gttcacttga catgtcctaa ctaattatca
       61 agcaaggaac attttcttcg ttagtaatgg attttaaaga aatttcagtt tttggatgtt
      121 gaataaaaga ccatttgtga aaccactagt atacatactc attaccatgt caattctatt
      181 actttaaaag aaaatcgttt tactttgaag aggagcatat ctatccatta aaagatgatg
      241 gtaatgcaat cactagttcg agtcatttta agagccatgc agtgagctgg aaataaaacg
      301 aatagaagat gggtttaaca aaaggaccag tcataggaag aaattgtcgt gaaatcgaat
      361 gataatctga acggtaggca aagactgtta aaaagatggg aaaggtatgt gcgatcattt
      421 tactgcacga ttatttggaa aataccttgt tgttcgcttt ttctggaaaa aatacataaa
      481 aaaagataaa acgattgcga actgcgtgta ttcggtcttt cgatcttttt cacggtatgg
      541 tatgatagtt aagaattttg agaaatttta aaaaataata atcgttacac aaaaatgttt
      601 tttaaagttt tcttatgatt aaaaagtgaa tttgctgatt gaacagttat atgagacaat
      661 attttagaaa atgtgaaaca gaggtgtatt atgaagcgat ggcaaaaagt tctattaacc
      721 ttgttagggg tattcgtttt gctgattgcc ggattatcag catacggaat cagatttatg
      781 ggagaagcga gccaaacagt aaacaaaatt tctaaaaact ctaatcgaat atcctccaaa
      841 cgttctgaga gagtaagcat tgatgacaaa gaaccttttt cggttttgct attaggttta
      901 gataccggtg gattaggaag aacggaacaa ggacgttctg atacgatgat ggtagtaaca
      961 gttaatccga aacaaaaaaa atcaacgatc atcagtttgg accgagatat ttatacgaat
     1021 atcgtaggtt atggaacggt agataaatta aatcatgcct acgcatttgg tggtgtcgaa
     1081 atggccatgg attcaattga gcaactgctc gatattccta ttgatcatta tgtaacgatc
     1141 aatttggatg gcatggaaga tttgatcaat gcggtcggtg gcatcaaagt gaataataaa
     1201 attgacttta ctttggatgg tgtccatgta ccagtaggtg aacaaacatt gaatggagaa
     1261 aaaggtttag cctattctcg aatgcgacac gaagatccag aaggcgatat cggtcgacaa
     1321 cgtcgtcagc gggaagtagt gacgaaaatc gtcaataaag ttttaagttt agatggcgta
     1381 agcaattata gaaaaatttt aaaagcagtt gagaacaatg tgacgacgga cttagattgg
     1441 gatgacatgc ttgatgtagc gacgaactac accccggcgt ttgaaacaat caaacaagac
     1501 cagttgcaag gcgaaggaca aatgatcaat gatatctact atcaaatttt aggaaaaaat
     1561 gatcttcttt cgatccaaaa tgaactgaag aaacaattaa atataaaaac aagcgataca
     1621 ttacctaatt tgaaaaatga gaatgcttca atgatgttct ttgatgattc ggaagatggc
     1681 gattcgacaa acggtgatgg atcgaatagt gaagggtaca ccgattattc ttcctattca
     1741 gaaaacacat atgatcagta ccaagatccg aatacctatt atcagcaaac agaagataca
     1801 agtcagtact atacaccaag tcaagatact caaagttatg atccttacgc tggacaggaa
     1861 caaaatggtt atggttatga ccagaataat gagtatggaa caacaagtgg gtactagaaa
     1921 aaaatagttt ggttaataaa gagtgccacc taaattagac aaaaaa
//
LOCUS       DAZUMI010000217         1963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000217 DAZUMI010000000
VERSION     DAZUMI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.226"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG81C_002924"
     CDS             64..972
                     /locus_tag="ACG81C_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS5066020.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACG81C_002925"
     CDS             972..1592
                     /locus_tag="ACG81C_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066021.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..>1963
                     /locus_tag="ACG81C_002926"
     CDS             1596..>1963
                     /locus_tag="ACG81C_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066022.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          697 a          302 c          443 g          521 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gac
//
LOCUS       DAZUMI010000218         1847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000218 DAZUMI010000000
VERSION     DAZUMI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.228"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG81C_002927"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG81C_002927"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5066023.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DAZUMI010000219         1802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000219 DAZUMI010000000
VERSION     DAZUMI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.229"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG81C_002928"
     CDS             275..1753
                     /locus_tag="ACG81C_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5066024.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          654 a          252 c          322 g          574 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gt
//
LOCUS       DAZUMI010000220         1793 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000220 DAZUMI010000000
VERSION     DAZUMI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.230"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG81C_002929"
     CDS             131..505
                     /locus_tag="ACG81C_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5066025.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG81C_002930"
     CDS             complement(619..1248)
                     /locus_tag="ACG81C_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5066026.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG81C_002931"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG81C_002931"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG81C_002932"
     tRNA            1668..1741
                     /locus_tag="ACG81C_002932"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          621 a          263 c          313 g          596 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt ttt
//
LOCUS       DAZUMI010000221         1770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000221 DAZUMI010000000
VERSION     DAZUMI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.231"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG81C_002933"
     CDS             <1..1087
                     /locus_tag="ACG81C_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5066027.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..>1770)
                     /locus_tag="ACG81C_002934"
     CDS             complement(1294..>1770)
                     /locus_tag="ACG81C_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS5066028.1"
                     /translation="LALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIIS
                     LDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMH
                     GLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          604 a          308 c          319 g          539 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa
     1441 ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc
     1501 tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa
     1561 aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc
     1621 aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa
     1681 ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat
     1741 taagtcaaag tctttccatt gtaacgctaa
//
LOCUS       DAZUMI010000222         1765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000222 DAZUMI010000000
VERSION     DAZUMI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.232"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..794
                     /locus_tag="ACG81C_002935"
     CDS             198..794
                     /locus_tag="ACG81C_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5066029.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(852..1334)
                     /locus_tag="ACG81C_002936"
     CDS             complement(852..1334)
                     /locus_tag="ACG81C_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066030.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1440..1637)
                     /locus_tag="ACG81C_002937"
     CDS             complement(1440..1637)
                     /locus_tag="ACG81C_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066031.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          595 a          358 c          269 g          543 t
ORIGIN      
        1 aaaaattcct aaaacacaac aatgaaagcg ataactaaga aaatccttac ataacgattt
       61 ttcttattaa aattgttttt tgaacttcgc ttattttttg tgagcatatc tgtttactta
      121 ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa aaaaatgcaa aacgcaaatc
      181 aaaggagaaa ttttattatg acaactttta ctgacacttt gaaaaaccgc cgttcaatct
      241 atcatctagg acgtaatgtt tcactttcta atgaagaatt aacaactttg atcaaagaag
      301 caattaaaga aagcccaact gctttcaatg ctcaatcaac acgcgcagtt atccttttcg
      361 gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc acttcgccca ttgactccag
      421 cagaagcttt cccaaataca caaaacaaat tagctggttt caaaaatggt tatggtacag
      481 tattattctt caaagatact gacgtggtaa aaggtctaca agaacaattt gaattatatg
      541 cagacaactt cccagattgg tcagaacaat caaacggaat cgcaacagct aatacttggg
      601 tagcattagt tgataaagga ttgggcgcaa acttgcaaca ttacaaccca gttattgacg
      661 aagcagttgc aaaagaatgg aatatccctt caaactggaa actacgttcg caattagtat
      721 tcggttcacc agaaacaccg gctgaggaaa aagaatacat gaacgatgcc gatcgcttcc
      781 gcgtatttgg ataattttat ttgaaataaa aaacagaggt tgtgacaaac atcttgtcgc
      841 aacctctgtt tttattctgg atcaatcata atatcaatct ctgcctcatc gtttaagcgt
      901 tgggacaaaa atggaactaa ttcttctaat gatgcagaaa atttcagcat cggtcctcgc
      961 catccgtttc ggaaaaaatt catttttacg attttagaac cttttggctg atataataca
     1021 attttattga tctcagaaaa aggaacacct tttttgtcaa agctattcaa aacaagtcct
     1081 tcttctgtga ttcctctact gtccaacaaa aaactaataa aaatcattgc tgacaagatc
     1141 ccttttgcca attcatctgc tgtaggatga ctgatgagtg tataccaaat gactagtgac
     1201 actaagaagg gtatcaccaa gtagacccat cgatttcgca cagtccgata aataatttct
     1261 tttcgaacca gccaaagaaa taaaaacatc acaacaacga ataaaaacaa tagaaccgta
     1321 aagaatgtta ccatgacaac tcgcctcgct ttttcttctc cagaaaatag gaaaagaata
     1381 ctgatttacc ttctcaaaga aattggactt gcagcacgcc gcacgtccaa ttgttttctt
     1441 tattcaaatg tatcatattt tgcaacaacc atgcttgctt tttcatctaa taattcttgg
     1501 tcctctgcag cacattgggc aactttgacc acggcgcagt ttgtcatttt tgatacaact
     1561 tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg catattgttc tcctaaatta
     1621 atcatttctt ccgtcatcaa aatcctccta aactgctgga acttttctcc acattaccac
     1681 tgctatttct ttggagattt aactcattga tttttattta cagttaaaaa tcaaccttac
     1741 aggcattata gcgtactgtt ttcgt
//
LOCUS       DAZUMI010000223         1755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000223 DAZUMI010000000
VERSION     DAZUMI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.233"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..446
                     /locus_tag="ACG81C_002938"
     CDS             192..446
                     /locus_tag="ACG81C_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5066032.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="ACG81C_002939"
     CDS             447..764
                     /locus_tag="ACG81C_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5066033.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..>1755
                     /locus_tag="ACG81C_002940"
     CDS             1323..>1755
                     /locus_tag="ACG81C_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066034.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT          625 a          249 c          327 g          554 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatga
//
LOCUS       DAZUMI010000224         1748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000224 DAZUMI010000000
VERSION     DAZUMI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.234"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1292
                     /locus_tag="ACG81C_002941"
     CDS             123..1292
                     /locus_tag="ACG81C_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5066035.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1632..>1748)
                     /locus_tag="ACG81C_002942"
     CDS             complement(1632..>1748)
                     /locus_tag="ACG81C_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5066036.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          608 a          289 c          290 g          561 t
ORIGIN      
        1 ttttacactc tctattatga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1441 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1501 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1561 ccatggataa taataagggt tctgttgcaa agttttaaat aaagaataaa atcccttacg
     1621 gtatctatga tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac
     1681 ccgaagagag tgccttcttt tcgggttttc ttatataatc ctcggatggc ttccatgcct
     1741 ttaatcgt
//
LOCUS       DAZUMI010000225         1742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000225 DAZUMI010000000
VERSION     DAZUMI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.235"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..1260)
                     /gene="tnpB"
                     /locus_tag="ACG81C_002943"
     CDS             complement(112..1260)
                     /gene="tnpB"
                     /locus_tag="ACG81C_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS5066037.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1277..1681)
                     /gene="tnpA"
                     /locus_tag="ACG81C_002944"
     CDS             complement(1277..1681)
                     /gene="tnpA"
                     /locus_tag="ACG81C_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5066038.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          522 a          298 c          274 g          648 t
ORIGIN      
        1 tgggagcttc ttgggaatag agcatacttg atagcgtatt tctttactaa cagcggtgtc
       61 tcttatttac caagctatcc ccgtagttcc tacggttctt gatgattgga tctaagccaa
      121 tcccatagaa tgtagtctta gaccttcggt cagaatattg atactagcat tgatatcacg
      181 atcatgatga gtatgacaaa caggacaagt ccattctcga atttcaagag atttcttgcc
      241 atctttatgt ccgcactctg aacaaatctg actagacgga aaccatttat ctactttgat
      301 gatttttcgt ccataccagt cagccttgta ttgtaattta gtcacaaact ttgaccaaga
      361 aacatcagaa atacttttag ctaatttatg atttcgcaac ataccttttg tgtttaagtc
      421 ttcaatacag ataatatcgt gatttttgat catttctgta ctcaacttat tcagaaaatc
      481 agtacgttga ttcattactt tttcatgcaa tctagctact ttgcgttttt gtttttgata
      541 atttttagct tcaaatagat tggtaccttt atttttagcc aataaagcac gcctagacaa
      601 tttgcgttgt tcacgcttta gtttcttttc cattttggac gtgaatttat tattatcaat
      661 tttttgtcca tcagaaagaa tcgcaaagtc cataatacct aagtcaatac caattgtgga
      721 attagtttta ggtaattcga taatttcttc tttacacaac aaagaaatat agtatttacc
      781 gctagaatga cgtgatattg tagcagattt gataatccct tttggttgtc tatgaagctt
      841 aattcttact aatgacttca atttaggaac tttgatgaat ttactatcaa tcaaagcaac
      901 tgtaccattt tgattattcg ttgtataact ctgaacagga tttttcttac ttttgaaacg
      961 tggaaatcca acggatttat cccgaaaaaa attcttgtat gctttatcta aattaagttg
     1021 ggcattggct aaagcaaggc tatcaatttc tttcaaaaac ggaaattctt tcttgtattt
     1081 agctggtgtc ggaaaagtca tttttttaga agaatcattt ttaacttctt catacgcctt
     1141 ttttcggtca tcaagcatta gattgtagac cttacggaca caaccaaaag atttggcaaa
     1201 gaagatttct tgttcctctg ttggatagat tctgaatttg tatgctttta gttgttccat
     1261 aaagttcacc tactttctat ttatgattgt ttccttgatt ttggatatac ttcttaatca
     1321 catcaattgg tgcgccacca gttgtcagaa gacaaaaact tttagaccag aacatttctt
     1381 tccaaagaaa ttgtttgact cttggaaagt cacgttttat caatcttgag ctggcacttt
     1441 tataggcatt gatgaatttt gtcaattctg ttttaggttg agctttgaac ataatatgaa
     1501 cgtggtcttt atcatgattc cactccacta aagtaatgtg atacgattct gaaattcttt
     1561 caaaagtagt tttggcataa tcagatattt catcatcaat cacttgtcta cgatatttcg
     1621 ttactaaaac aaggtggtaa taaagaagaa atactgaatg attatttgta tctaattcca
     1681 tgttatatca actcatttct tgtatagact gattgtaaca tggaacaaaa taaaaaggca
     1741 at
//
LOCUS       DAZUMI010000226         1724 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000226 DAZUMI010000000
VERSION     DAZUMI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.236"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..588
                     /locus_tag="ACG81C_002945"
     CDS             22..588
                     /locus_tag="ACG81C_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5066039.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            733..820
                     /locus_tag="ACG81C_002946"
     tRNA            733..820
                     /locus_tag="ACG81C_002946"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:765..767,aa:Ser,seq:cga)
     gene            1154..>1724
                     /locus_tag="ACG81C_002947"
     CDS             1154..>1724
                     /locus_tag="ACG81C_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS5066040.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          585 a          267 c          340 g          532 t
ORIGIN      
        1 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
       61 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
      121 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
      181 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
      241 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
      301 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
      361 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
      421 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
      481 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
      541 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
      601 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
      661 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
      721 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
      781 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
      841 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
      901 tttttcgttt gattgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
      961 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     1021 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     1081 gccttttttt gataaaaaaa atactagatg taagataaaa gtgtaaaaac gatttgtttt
     1141 aggaggggaa acgatgtgta ccgcaatcac ttatgcaacg aaagaccatt attttggaag
     1201 gaattttgat tatgagatgt cttataatga agtggtcact attactccaa gaaattatcg
     1261 tttcgatttt agaaaggtaa aaaatttaga taaacattat gcaatgatag gaatagcagc
     1321 tggggtatcc aactatccac tctattatga ggctacaaat gaaaaagggt tgagtatggc
     1381 aggactgaat tttcctggca atgctgatta taaagaatta caagaaggaa aagataacgt
     1441 tgcaccattt gagtttattc catggatact agggcaatgc tcaaccatag acgaagctaa
     1501 agaattattg gctactatca atctagtcaa tatcgatttt agtgaaaaac tacccttgtc
     1561 acctttacac tggttattag ctgataaaga aaaatcgata gtaattgaaa gtatgaaaga
     1621 tggacttcat ttatatgata atcctgtcgg tgtgttgaca aataatccgc catttgatta
     1681 tcaattattt aatctgaaca attatcgctc gctatcaaat ggaa
//
LOCUS       DAZUMI010000227         1696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000227 DAZUMI010000000
VERSION     DAZUMI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.237"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG81C_002948"
     CDS             complement(54..788)
                     /locus_tag="ACG81C_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5066041.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG81C_002949"
     CDS             1115..1525
                     /locus_tag="ACG81C_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5066042.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..>1696
                     /locus_tag="ACG81C_002950"
     CDS             1518..>1696
                     /locus_tag="ACG81C_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5066043.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYN"
BASE COUNT          544 a          295 c          325 g          532 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatat
//
LOCUS       DAZUMI010000228         1653 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000228 DAZUMI010000000
VERSION     DAZUMI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.238"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1025
                     /locus_tag="ACG81C_002951"
                     /pseudo
     CDS             115..1025
                     /locus_tag="ACG81C_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          623 a          187 c          336 g          507 t
ORIGIN      
        1 aactgtcatt tggttatcac ggcgatacta aaattaacaa aattggaata tttttcttga
       61 agatttttag ctgtcagtat atgatgaaag catctcatag gaggtgcttt tttcatggtt
      121 gataattcaa gtggaagggt tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa
      181 aagttagctg atttagcagg tatttcacaa agtactttag ttaaatatga aaaagggtca
      241 agaaaaatcc ctaaagacgt agataatact ctaagcaaga ttctgaatat agaaactcta
      301 ataaaagatg aggaagataa gattgagatt ctcatcggta aactaattgc atacagagat
      361 atgaataagt tacttaacaa agagttagct gataatatag gaattagtga agtattactc
      421 agttatgtgt taaatagaaa gagaaatccg tcaaaagaaa tgcaaaaaaa atcgatattt
      481 ttctattaag taatgaaaaa gaaattttga aggagattaa tcgtgatagt gagatattta
      541 gtttatcaaa agacgataaa attgttatgg gaaaaagaat acgagaagtc cgaaagaacc
      601 gggaagagac gcttgaaaaa tttggtaaaa actttaccat ctatacaggc aaaaatgtta
      661 taagtagatg ggaaaaaggt attaatattc cagatattga aaaactaatg aatattgcct
      721 atcttggtaa ggtcactgtt ccttacctta tgtacggaga agattataag aatatacttc
      781 caaaagatga aagagttagc gattttaaaa agataaattc ttttagtatg ggattaagga
      841 tgaggaaaat taggaaagat tattatctag aaagggagga gtttggaaag cttttttctc
      901 cttcaatttc aaaatggtcc atagataggt atgagaatgg tagagatata cccaatacta
      961 atcgaattat ccaatatgct tatataggaa atctttcttt agaatttttg atttacggga
     1021 tataaaaata gtttacgtcg atacttatta agttatcgac gttttttatt atagaaaaaa
     1081 tttataaaag gaggtttctt gatatgataa gaaacaaaaa gggtttgttg aaagttgctt
     1141 tactggcagc ttttttctaa gtaacttttt gacaagctca atggcaacac aagcggaaga
     1201 agtaatacaa gaactaactg ttatgaataa cagctagata aaatgggcta tgtatgttat
     1261 ccaacaattt agtaatcact agaagcctag cgaaagttcg cttgataagt tatagatgag
     1321 tattgatttg tagtgtttcc aaatcaaaag ggtacagagc cacatgaaat atgttgtcct
     1381 ctgcagcgtc aggatgggaa tccataccat ggagattcgg agatctgtga gggaatactt
     1441 acgtggttaa tatgtcagtt gtgttggacg tagtaatggt catagtgggt agattgactg
     1501 aattcttata tgagaaaatg aaaccaacaa gaagataggg ttatttatac agaattgggg
     1561 atcttttctg gaaaagtaat cggataatgc gagattcgta cggtcttttt taaatttctc
     1621 taaaaaaaac ggcatcgaat gggttgggtg att
//
LOCUS       DAZUMI010000229         1647 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000229 DAZUMI010000000
VERSION     DAZUMI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.239"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG81C_002952"
     tRNA            complement(20..91)
                     /locus_tag="ACG81C_002952"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG81C_002953"
     tRNA            complement(113..200)
                     /locus_tag="ACG81C_002953"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG81C_002954"
     tRNA            complement(225..298)
                     /locus_tag="ACG81C_002954"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG81C_002955"
     tRNA            complement(312..382)
                     /locus_tag="ACG81C_002955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG81C_002956"
     tRNA            complement(384..459)
                     /locus_tag="ACG81C_002956"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG81C_002957"
     tRNA            complement(478..551)
                     /locus_tag="ACG81C_002957"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG81C_002958"
     tRNA            complement(564..653)
                     /locus_tag="ACG81C_002958"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG81C_002959"
     tRNA            complement(677..750)
                     /locus_tag="ACG81C_002959"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG81C_002960"
     tRNA            complement(764..837)
                     /locus_tag="ACG81C_002960"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG81C_002961"
     tRNA            complement(865..937)
                     /locus_tag="ACG81C_002961"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG81C_002962"
     tRNA            complement(957..1030)
                     /locus_tag="ACG81C_002962"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACG81C_002963"
     tRNA            complement(1040..1113)
                     /locus_tag="ACG81C_002963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACG81C_002964"
     tRNA            complement(1124..1209)
                     /locus_tag="ACG81C_002964"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACG81C_002965"
     tRNA            complement(1226..1297)
                     /locus_tag="ACG81C_002965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACG81C_002966"
     tRNA            complement(1309..1381)
                     /locus_tag="ACG81C_002966"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACG81C_002967"
     tRNA            complement(1399..1480)
                     /locus_tag="ACG81C_002967"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACG81C_002968"
     tRNA            complement(1488..1560)
                     /locus_tag="ACG81C_002968"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACG81C_002969"
     tRNA            complement(1564..1636)
                     /locus_tag="ACG81C_002969"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZUMI010000230         1640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000230 DAZUMI010000000
VERSION     DAZUMI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.240"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..>1640
                     /gene="ltrA"
                     /locus_tag="ACG81C_002970"
     CDS             416..>1640
                     /gene="ltrA"
                     /locus_tag="ACG81C_002970"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5066044.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          586 a          299 c          388 g          367 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag
//
LOCUS       DAZUMI010000231         1619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000231 DAZUMI010000000
VERSION     DAZUMI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.241"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1367
                     /locus_tag="ACG81C_002971"
     CDS             <1..1367
                     /locus_tag="ACG81C_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HFS5066045.1"
                     /translation="DNFYKKFIDEGSKNYVGYDDFINQTKDIIFVPYLLLQKIIETKK
                     DINEGIENISDSKISPVSSIITEQTQIDISELEDKHAIQQNDHFSEHNEFIERFNQLT
                     IRSKLHYSKRDLWNFHTAMLGDSLVVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISV
                     RPFWEDDSDLLGYADTVNSVYRPGDSGLIDALLEASRHKENIYMVCFDEMNLARVEHY
                     FSQFLSVLEMEPNTRKIKLYNEDLENKLYNSSTYPSTIEVGQNILFVGTINTDESTHQ
                     FSDKVLDRSNIISLELVPFHEISEVNDYIQSDQKKRNQLKYDDFKNFKNSNPTNGLTQ
                     KEKEMFWKLHLAINSKDKNIGIGWRILKQIDEYLKNLPVQNDLSRKEAIDIQLVQRVL
                     SKIRGSDEQLSELVGKWNENDLKQVGEFEQIINEYPESSDFKYTKKILLQKARELNLH
                     GFTL"
     gene            1351..>1619
                     /locus_tag="ACG81C_002972"
     CDS             1351..>1619
                     /locus_tag="ACG81C_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066046.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNII"
BASE COUNT          641 a          210 c          255 g          513 t
ORIGIN      
        1 aagataattt ttacaaaaag tttattgatg aaggtagtaa aaactatgta ggttacgatg
       61 attttataaa tcaaacaaaa gatataattt ttgtgcccta tctattactt caaaaaatta
      121 tagaaactaa aaaagatatt aacgaaggca tagaaaatat ctctgattcc aaaatatctc
      181 cagtaagttc aattattact gaacaaacgc aaatagatat atctgagtta gaagataaac
      241 acgctattca acaaaatgat cacttctctg aacataatga atttatagaa agatttaatc
      301 agctcacaat tcgtagtaaa ttacattact caaaacgaga cttatggaat tttcatactg
      361 ctatgttggg tgatagtttg gtggtattag ctggactaag tggtaccggt aaatcacaat
      421 tagtaacatc ctatgcaaaa gcacttcaat tatcagatac acaaataaag tttatttctg
      481 tgagaccctt ttgggaggat gattctgatt tattgggcta tgctgatact gtaaattctg
      541 tctatcgtcc aggagatagc ggtctaattg atgcactatt agaagcatca agacataaag
      601 aaaatattta tatggtttgt tttgatgaga tgaatttagc tcgggttgaa cattatttct
      661 cccaatttct ttcagtttta gaaatggagc caaatactag gaaaatcaaa ctgtataacg
      721 aagatctaga aaataaatta tataactctt caacctatcc ttctacaatt gaagtaggac
      781 aaaacatatt atttgtagga acaataaata cagatgaatc tactcatcaa ttctcagata
      841 aagttttgga tagatcaaac attatttcac ttgagttagt accattccat gaaattagtg
      901 aagtaaatga ctatattcaa tcagatcaaa agaaacgtaa tcaacttaaa tatgatgatt
      961 tcaaaaattt taaaaactct aaccctacta atggtttgac ccagaaagaa aaagaaatgt
     1021 tttggaaact ccatttagca attaattcta aagacaaaaa tatcggtatc ggttggagaa
     1081 tattaaaaca aattgatgaa tatttaaaaa atcttccagt gcaaaatgac ttatcaagaa
     1141 aagaagctat tgatattcag cttgtacaga gagtattaag taaaatcaga ggttctgatg
     1201 aacaattatc agaattagtt ggtaaatgga atgagaatga tttaaaacaa gtgggtgaat
     1261 ttgaacaaat aattaatgaa tatcctgagt cttcagattt taaatatact aaaaaaattc
     1321 ttttgcaaaa agctagggag ttgaatttac atggatttac cctttagtct gaaattaaac
     1381 tttggttata aaagtgaaga acaagaatat gaacttttat tttttcaaga tgaggcatcg
     1441 ttattagagt atacaccggt taaggtaaaa gaattttcta atctaagctt tacatttaac
     1501 agtatagaca aacacgcaaa actatatata ccagaattag atgattattt gattgaaaat
     1561 caagtaaaag aggaaaatgg agatatttgc tatattccaa gtgatgataa tataatcat
//
LOCUS       DAZUMI010000232         1617 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000232 DAZUMI010000000
VERSION     DAZUMI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.242"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..1226
                     /locus_tag="ACG81C_002973"
                     /note="aph(2'')-Ia"
                     /pseudo
     CDS             126..1226
                     /locus_tag="ACG81C_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
     gene            complement(1356..>1617)
                     /locus_tag="ACG81C_002974"
     CDS             complement(1356..>1617)
                     /locus_tag="ACG81C_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066047.1"
                     /translation="PKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVR
                     RREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          635 a          161 c          277 g          544 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga aatgctaatg
      181 cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac caaaaatctg
      241 gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa aaagaagatt
      301 gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca atgaaatatt
      361 taattgagca ttactttgat aatttcaaag tagatagtat tgaaataatc ggtagtggtt
      421 atgatagtgt ggcatattta gttaataatg aatacatttt taaaacaaaa tttagtacta
      481 ataagaaaaa aggttatgca aaagaaaaag caatatataa ttttttaaat acaaatttag
      541 aaactaatgt aaaaattcct aatattgaat attcgtatat tagtgatgaa ttatctatac
      601 taggttataa agaaattaaa ggaacttttt taacaccaga aatttattct actatgtcag
      661 aagaagaaca aaatttgtta aaacgagata ttgccagttt tttaagacaa atgcacggtt
      721 tagattatac agatattagt gaatgtacta ttgataataa acaaaatgta ttagaagagt
      781 atatattgtt gcgtgaaact atttataatg atttaactga tatagaaaaa gattatatag
      841 aaagttttat ggaaagacta aatgcaacaa cagtttttga gggtaaaaag tgtttatgcc
      901 ataatgattt tagttgtaat catctattgt tagatggcaa taatagatta actggaataa
      961 ttgattttgg agattctgga attatagatg aatattgtga ttttatatac ttacttgaag
     1021 atagtgaaga agaaatagga acaaattttg gagaagatat attaagaatg tatggaaata
     1081 tagatattga gaaagcaaaa gaatatcaag atatagttga agaatattat cctattgaaa
     1141 ctattgttta tggaattaaa aatattaaac aggaatttat cgaaaatggt agaaaagaaa
     1201 tttataaaag gacttataaa gattgattat ataatatatg aaaagctatt ataaaagaca
     1261 ttagtattaa atagtttaaa aaaatgaaaa ataataaagg aagtgagtca attccagact
     1321 cctgtgtaaa atgctataca atgtttttac catttctact tatcaaaatt gatgtatttt
     1381 cttgaagaat aaatccattc atcatgtagg tccataagaa cggctccaat taagcgattg
     1441 gctgatgttt gattggggaa gatgcgaata atcttttctc ttctgcgtac ttcttgattc
     1501 agtcgttcaa ttagattggt actctttagt cgattgtggg aatttccttg tacggtatat
     1561 tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag cttttgaata ttttggt
//
LOCUS       DAZUMI010000233         1617 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000233 DAZUMI010000000
VERSION     DAZUMI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.243"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG81C_002975"
     CDS             complement(<1..613)
                     /locus_tag="ACG81C_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5066048.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..1554
                     /locus_tag="ACG81C_002976"
     CDS             901..1554
                     /locus_tag="ACG81C_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5066049.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          581 a          225 c          299 g          512 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa
     1501 ttagactgta taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat
     1561 taaagatatt tccaaaaatg ttcatacaaa tattttctgt tcttggaata ataatta
//
LOCUS       DAZUMI010000234         1602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000234 DAZUMI010000000
VERSION     DAZUMI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.244"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..259
                     /locus_tag="ACG81C_002977"
     CDS             <1..259
                     /locus_tag="ACG81C_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066050.1"
                     /translation="NNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDV
                     DRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(621..860)
                     /locus_tag="ACG81C_002978"
     CDS             complement(621..860)
                     /locus_tag="ACG81C_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066051.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1146..1460
                     /locus_tag="ACG81C_002979"
     CDS             1146..1460
                     /locus_tag="ACG81C_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066052.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          549 a          219 c          281 g          553 t
ORIGIN      
        1 gaataacgaa actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca
       61 aaaagtgatt ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga
      121 atatcatgac gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata
      181 tactcacgtt tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga
      241 acatacttta gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct
      301 tttgaaggca ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg
      361 ttcgcctaga gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat
      421 actgccagaa ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga
      481 tccaatcatt tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc
      541 gtaaagcatt tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt
      601 tgctgaagaa gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac
      661 agtttcttta ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga
      721 gaatttttct catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa
      781 ggtatgttct ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg
      841 ataaacagaa ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta
      901 tgctcagtat taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc
      961 ttagtaatac atatatggag agggtgatat cccagttcaa accagataca ttggttacca
     1021 tctgtattaa cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac
     1081 gtaggtatga tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat
     1141 agcttttgga tttttacagt aattttatct taataatagc aatattatta cttctaaata
     1201 tttggttttt tgataagagt agaaatgcag ggataggttt tagaaccaaa cgaagtacgt
     1261 cttctgaaaa aaagtgggta tattcacaga ctatttttta cggaggggtt atttcaatca
     1321 gtttactttc ttcaacactt tattctttta atgttataga cgtttccatg tcaaatttta
     1381 tatctattat tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag
     1441 agaagtcaaa aaataattaa agcaattatg ccttttagta caggaattat tacttcttac
     1501 aaatgtatag cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa
     1561 caattatttt atgttaggat gaattaattt ctctagagtt ta
//
LOCUS       DAZUMI010000235         1595 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000235 DAZUMI010000000
VERSION     DAZUMI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.245"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..696
                     /locus_tag="ACG81C_002980"
     CDS             289..696
                     /locus_tag="ACG81C_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066053.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="ACG81C_002981"
     CDS             710..1111
                     /locus_tag="ACG81C_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066054.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="ACG81C_002982"
     CDS             1113..1484
                     /locus_tag="ACG81C_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066055.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          521 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtcc
//
LOCUS       DAZUMI010000236         1537 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000236 DAZUMI010000000
VERSION     DAZUMI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.248"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG81C_002983"
     CDS             complement(52..1518)
                     /locus_tag="ACG81C_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS5066056.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          501 a          326 c          289 g          421 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctt
//
LOCUS       DAZUMI010000237         1509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000237 DAZUMI010000000
VERSION     DAZUMI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.249"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            894..1106
                     /locus_tag="ACG81C_002984"
     CDS             894..1106
                     /locus_tag="ACG81C_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5066057.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1096..1491
                     /locus_tag="ACG81C_002985"
     CDS             1096..1491
                     /locus_tag="ACG81C_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066058.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          528 a          223 c          252 g          506 t
ORIGIN      
        1 atctagatct attaataata ctgatcccta ataggtgtaa aacagttata agaaatgagt
       61 actctagtat ttgtgttacc cgaataatta cttgattata ttggtttccc atataattga
      121 aggctgtcct aatttttacc tactatatga tcataattga ttaactctta tatatttccg
      181 aaaattatct tgaagatttc tattggtatg gagaatatcc atttcattgc caataaggat
      241 ataaattctc aaaataacaa tcgatgtctg tattaaacaa gcctagaatg gtaaatctga
      301 ttcgccaaaa ccattggatt gtatcgattt gctttacgcg aacgccacct acccatttta
      361 taaaaataaa atgggcaaaa gatctttctt tttgtcatac aaaaagaaga aaaaaaagag
      421 acgattttct tcttccaaaa tcgtccctct tttttctaaa accatggatg aacgaacaaa
      481 aaatctctcg tattttgttt gttcatccac taaaaatatg cgaacagtat ggtttcattc
      541 aatcctgttc ttacaagtca aaagcatcga atcgtccttg gacgattcaa caaaaaaagt
      601 agtcgttttt ggatgactac taagttctaa acattcatta cccaaaatca atttggtcta
      661 acatcgtttc tcgactggct tgatctaatc ggctcttgac agggcttttt cttttctgtt
      721 gatagcagat aagaggaaat aagaataagt gtgttcatgt gtcataaaaa cagtaactaa
      781 aacattaaaa tcaaaattta tatggaaagc ttccttgaca ttttgtgctc gcaggagtat
      841 tataaaagca cgaaaggaaa gtaaactttc cattttttag aaaggagcat gttgtgaaaa
      901 atcgtttgga agaattacga aaacaaagag gaattaaaca agaagattta gcttctgctc
      961 ttgaagtatc tcgtcaaaca ataggttctt tagaaaatgg acgttataat ccctctatta
     1021 tattggcatt taaaattgca aggtactttc aaatgagcat tgaagaaatt tttatctatg
     1081 aggaggaaag tgaagatgaa aaataagggt cagatttggt atatcggtta tattgtttcg
     1141 ttgtttttga ttgtgacaat tttggttaca gattttccta aaatggtaga tattggactt
     1201 gctattttat tcagtgccgt ttttggcgtt tctcatgttc aaattttgca tgataaaatg
     1261 attaaaaatg atgcagacta caaaatcaat gttatggacg aacggaatat cataatcaaa
     1321 gaaaaagcgg gaaatataac aaatatgttt aacacaatac tgttaggggt ggcaacagtt
     1381 atatttattt gtttagacta catcattcca gctattattg tcggaagtat cattgcaatc
     1441 cagccgatta ttttaatcat tatcagtagt gtgcttgaga aaaaaatgta gaaataaagg
     1501 ttcatacaa
//
LOCUS       DAZUMI010000238         1501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000238 DAZUMI010000000
VERSION     DAZUMI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.250"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACG81C_002986"
     CDS             complement(<1..125)
                     /locus_tag="ACG81C_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFS5066059.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ACG81C_002987"
     CDS             complement(143..463)
                     /locus_tag="ACG81C_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5066060.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..>1501
                     /locus_tag="ACG81C_002988"
     CDS             762..>1501
                     /locus_tag="ACG81C_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5066061.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          557 a          258 c          234 g          452 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
     1021 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
     1081 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
     1141 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
     1201 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
     1261 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
     1321 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
     1381 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
     1441 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
     1501 g
//
LOCUS       DAZUMI010000239         1480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000239 DAZUMI010000000
VERSION     DAZUMI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.251"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG81C_002989"
     CDS             64..972
                     /locus_tag="ACG81C_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS5066062.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACG81C_002990"
     CDS             972..>1480
                     /locus_tag="ACG81C_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066063.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAZUMI010000240         1474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000240 DAZUMI010000000
VERSION     DAZUMI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.252"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1434
                     /locus_tag="ACG81C_002991"
     CDS             94..1434
                     /locus_tag="ACG81C_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5066064.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          465 a          325 c          284 g          400 t
ORIGIN      
        1 caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct ttagaataaa
       61 gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag
      121 ttactagata ttctagacct aaatcttacc tttcatgaag atgcctttcg caaagagcgc
      181 attaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc agaggaatgc
      241 ttccattgcc actatcaaaa taagcaaacg atcattaaat ggggctggaa aaaagtatcc
      301 atcttattga atgatgtcag taactacaag accattgtgc ggattaacaa acagcgcttc
      361 aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagcga tcgtcgctgc
      421 tccattgccc gcagagtcaa acaagccatt cttgagctat taagtgagcc tatttctatg
      481 tctttgattg cacgtatgaa gcacatttct ccgaccactg tcattcgaat acttcgcagc
      541 cttcggccta aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg tttcgatgaa
      601 ttcaaatcgg tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga cggcaagacc
      661 catcaactga ttgatattgt ggaaaatcgg caactcaatc ctttacgcga ctatttcttg
      721 cgctacccta gaaaagtccg tgaacaagta cgtctagtgg tttcagattt ctatacaccc
      781 tatcgcacat tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat
      841 attagccaac acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat gaagaccttt
      901 aaaaaagggg atcgtcggtc gaagcactta aagaaatact ggagactact tcaaaagaat
      961 gcgtgggaat tgaatggtca gcatcgctac tggcgtccgt catttcgaga tcatctgaca
     1021 gaagcggaga tagtcgatcg gctattatat tatgatgact ccttaaagcg aggctacgaa
     1081 gtctaccagg tttttctttc cgctatcaga agacaagatg ttcctgaatt cgtcgcgctc
     1141 ctaaaagaag actacaagga gttgccagag cactatcaac cagtgttcac gaccttcaag
     1201 aaatatcgaa cagaaatcaa acgagcgttg cgagttccct attccaacgg ccctatagaa
     1261 tgtttgaaca accatatcaa agtactaaaa cgaattgcct atggctttag aaactttcaa
     1321 aactaccgag aacgaatctt tttatatcga ggaaaatatt tcaagaaaac taagaacact
     1381 acccaactga ctaaagccag aacaacaaca cggctagaca agatagctgt ctagccgtgt
     1441 gagtatctcg attattctat tgaatttctt cacc
//
LOCUS       DAZUMI010000241         1464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000241 DAZUMI010000000
VERSION     DAZUMI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.253"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..400
                     /locus_tag="ACG81C_002992"
     CDS             2..400
                     /locus_tag="ACG81C_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5066065.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="ACG81C_002993"
     CDS             384..1079
                     /locus_tag="ACG81C_002993"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5066066.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          553 a          213 c          294 g          404 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctac
//
LOCUS       DAZUMI010000242         1429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000242 DAZUMI010000000
VERSION     DAZUMI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.254"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            557..>1429
                     /locus_tag="ACG81C_002994"
     CDS             557..>1429
                     /locus_tag="ACG81C_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5066067.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKKGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          499 a          251 c          226 g          453 t
ORIGIN      
        1 gcgtaatttg tagccattaa tacatctcct tattcataat aaatttcata tgacgctcct
       61 ctcaaaattt accggtagac tagaaaaaag aataaaagat acgtcaattc acactttatt
      121 cttaaaaaaa actggtctct ctaccggtaa tatcgattga gtccgccttt atcgacttta
      181 tccttttttt atattaaatg ttttcaccgg tagacaggga gagaaagaat aaactaaaag
      241 cccttaattc ttaatgtttt cacctcctta ccgttcctac cgctaaaatt taatataact
      301 aaattctata acattagaag aaattttaaa tacctttgat aaaaaatatt gatgtttttt
      361 ctctcattgt ttacaaacta gatagcttat gtattcttat taaaaataat agatatttcg
      421 aacatcatca ctctcatcta cttaatttcg catttttact ttacgccgat tgtaaaatta
      481 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      541 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      601 gtcttatttc aaaattaatc aatctgttgc taaaactgcc cattgcttga atcgttcaag
      661 acaaacgatc cataaagtat atctgttctt caagaaagga aaatcagcct tagaatatta
      721 tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt tacccgagga
      781 acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg tgattgttgg
      841 tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt tcaaaaaggg
      901 gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg gacatcaaga
      961 aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg attatagcca
     1021 attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga aacataaaag
     1081 tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga aaccgtgtgg
     1141 tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg taccgaaaaa
     1201 cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga aacagatcag
     1261 taatgtcaat gatattgcca tttatttcgc tgatccagga acgccgtctc aaagaggcct
     1321 aaacgagaat tctaacggat tgttacgtag agatggttta ttgaaatcta tggatttcaa
     1381 ttcagtagat gaatttttta ttcaatctgt cgcatctaaa cgaaataat
//
LOCUS       DAZUMI010000243         1421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000243 DAZUMI010000000
VERSION     DAZUMI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.255"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACG81C_002995"
     CDS             <1..1192
                     /locus_tag="ACG81C_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5066068.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          563 a          204 c          303 g          351 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat a
//
LOCUS       DAZUMI010000244         1400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000244 DAZUMI010000000
VERSION     DAZUMI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.256"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            340..1380
                     /locus_tag="ACG81C_002996"
     CDS             340..1380
                     /locus_tag="ACG81C_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5066069.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          549 a          188 c          225 g          438 t
ORIGIN      
        1 gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt tcttaattta
       61 ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta aattttattc
      121 atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag ttcttaatga
      181 ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa
      241 cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat
      301 tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt
      361 aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct
      421 gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga
      481 ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat
      541 acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa
      601 aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta
      661 gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc
      721 tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg
      781 aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt
      841 atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa
      901 cttaataata atatattaga cactaataga cacaatatag acactgaaaa agaccgccta
      961 caagatcaat tgttgttaga caattttgag acaattatga caaacgacag cattgctacg
     1021 tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct
     1081 caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga aagtgatatt
     1141 tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg
     1201 acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg
     1261 atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt
     1321 gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg gttagagtga
     1381 aactttactg acaaagtgat
//
LOCUS       DAZUMI010000245         1397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000245 DAZUMI010000000
VERSION     DAZUMI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.257"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..>1397
                     /locus_tag="ACG81C_002997"
     CDS             122..>1397
                     /locus_tag="ACG81C_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5066070.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHG"
BASE COUNT          435 a          276 c          266 g          420 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat
     1381 tatgattcgt catggca
//
LOCUS       DAZUMI010000246         1387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000246 DAZUMI010000000
VERSION     DAZUMI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.259"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..>1387
                     /locus_tag="ACG81C_002998"
     CDS             join(53..494,494..>1387)
                     /locus_tag="ACG81C_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS5066071.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSI"
BASE COUNT          580 a          182 c          242 g          383 t
ORIGIN      
        1 gatgtgcacc ccaaaagtta gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa
       61 atatacattt gtttttaaga aaaaagtagt ttcagactat ttaaataacg aaggcggcta
      121 caaatatctt gcacataaat atcaaataaa tcgtacactg gttagacatt gggtaaggat
      181 ttataactat catggttggg aaggcttggt tggaggtggc aaaagctaca ctacaaaatt
      241 taaacttgat gttatagaat atatggaaac aaatggtctt tctatccaag aaactgctaa
      301 aaaatttaat atcggttcaa atagaactct aagtaaatgg atagagcaat atgaagaagg
      361 cggtgcttct tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct
      421 aaacattcct aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc
      481 agagaacgca tatttaaaaa agttagaaac cttgattcaa gaacaagatt tgagcaagaa
      541 gaaatcaagg ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa tctattactt
      601 tcaattgcac aattagcgaa gtcaacttat tattactggg taaaaaaatt agataagcca
      661 gataaatata gcaagataaa gcaagaaatt acagcgattg taaaagaatc aagaaactct
      721 tatggttatc gtagagtcac tttagcgtta aagatgaaag gatatacaat caatcataaa
      781 acagttagaa aattaatgtc ccaaatggga cttacctgtc aaatccgaat aaagcgatat
      841 aaatcttata aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg aaatttttca
      901 gtagatacac ccaataaaaa atgggtgaca gacgtcactg aattcaagat aaaaggaaga
      961 aaaatctatc tatctcctat acttgattta tttaacggag aaataattag ttatagtata
     1021 tcaactagtc caacatataa actgattgaa gaaatgcttc aacaagcaat taaaaaaaag
     1081 ggaactgagg gttcactgat tctacattca gatcaaggat ggcagtatca gatgccacaa
     1141 tatcaaaaaa aattaaaaga gaataacatt attcaaagca tgtctagaaa aggaaattgc
     1201 ttagataatt ctgtaataga aaattttttt ggtgtgctta aatcagagtt tttttatcga
     1261 gaaaaattcc gatcaattga gatatttcaa agtaaattaa atgagtatat taggtggtat
     1321 aataacaaaa ggataaaact aaagttaaat ggactatctc cagtagaata tcggaaacag
     1381 tccatta
//
LOCUS       DAZUMI010000247         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000247 DAZUMI010000000
VERSION     DAZUMI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.260"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG81C_002999"
     CDS             82..>1364
                     /locus_tag="ACG81C_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5066072.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZUMI010000248         1340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000248 DAZUMI010000000
VERSION     DAZUMI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.261"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG81C_003000"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG81C_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG81C_003001"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG81C_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          481 a          218 c          180 g          461 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa
//
LOCUS       DAZUMI010000249         1329 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000249 DAZUMI010000000
VERSION     DAZUMI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.262"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..982
                     /gene="scrK"
                     /locus_tag="ACG81C_003002"
     CDS             110..982
                     /gene="scrK"
                     /locus_tag="ACG81C_003002"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5066073.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          410 a          234 c          309 g          376 t
ORIGIN      
        1 aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta aatatgatat
       61 atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta tgctatacgg
      121 tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat
      181 caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt tagtcattga
      241 cttcttcaaa caataccaag cagatttagc agggattggg attggctcat ttgggcctat
      301 cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga aattggcatg
      361 gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac
      421 aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag
      481 cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg
      541 ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa
      601 agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg
      661 acccgccatc gaaaaccgct tagggaaaaa aggacaagag gttgcagaag atgatcctta
      721 ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc
      781 tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt
      841 ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata
      901 tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg cattggctcg
      961 agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg ttggtagaga
     1021 aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt
     1081 ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta tggaaatttc
     1141 gatagccgta ggccacttgt attgtgcaat agttttttac aaaaaaaagg gggtgcggat
     1201 atttttttgg acctttttaa caaataggtt ctgttgcaaa gttttaaatc tactatcaga
     1261 taaggtagaa taatagaaaa agatagcagg aggaatgacg atgagtcatt ttaaaggaaa
     1321 gcaatttca
//
LOCUS       DAZUMI010000250         1328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000250 DAZUMI010000000
VERSION     DAZUMI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.263"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..289
                     /locus_tag="ACG81C_003003"
     CDS             <1..289
                     /locus_tag="ACG81C_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5066074.1"
                     /translation="RIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYI
                     FDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            294..1241
                     /locus_tag="ACG81C_003004"
     CDS             294..1241
                     /locus_tag="ACG81C_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5066075.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          444 a          189 c          273 g          422 t
ORIGIN      
        1 aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta ggcaacagac
       61 acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat ttctaaaaaa
      121 aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt atctaatttt
      181 agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta aatttttaga
      241 agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa aggatgagct
      301 gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat catttaggag
      361 aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa aatcttgata
      421 tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg atggaagaga
      481 tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct agtgtggcag
      541 attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt
      601 tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt tcttcagcgg
      661 ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt cgcccattga
      721 cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat tgtcatttat
      781 atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat caaaatccga
      841 attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa caaatcgtag
      901 gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac tttgtttata
      961 tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca ctggggaaac
     1021 aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat gcaatcaatg
     1081 aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt gatatccatg
     1141 actctctagc agatattact aagatccagt caattggcta tcaaccgaaa tatggtattt
     1201 taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta agataagatt
     1261 agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa atcaaatctg
     1321 ggatagaa
//
LOCUS       DAZUMI010000251         1315 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000251 DAZUMI010000000
VERSION     DAZUMI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.264"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            389..721
                     /locus_tag="ACG81C_003005"
     CDS             389..721
                     /locus_tag="ACG81C_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066076.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            631..834
                     /locus_tag="ACG81C_003006"
     CDS             631..834
                     /locus_tag="ACG81C_003006"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5066077.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            865..1011
                     /locus_tag="ACG81C_003007"
     CDS             865..1011
                     /locus_tag="ACG81C_003007"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066078.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1018..1173
                     /locus_tag="ACG81C_003008"
     CDS             1018..1173
                     /locus_tag="ACG81C_003008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFS5066079.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          472 a          233 c          231 g          379 t
ORIGIN      
        1 tctaaaacga atgattttaa ttattaacgt tttttaattc gtttttagct taaaaataac
       61 ttttgtcaca gtctcactcc taaaattact ttatttacgt acgaagtggg caggaggtcg
      121 aatccagaac tgggccatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg
      181 cgatttacct tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc
      241 tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca
      301 cagtttagtt gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca
      361 gttacgttac cggaaggaat gaactctatt gaaaaaaaca tatgatcgta aatttaaact
      421 aaagatttca caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata
      481 caatatctct cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa
      541 tgcttttgct ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca
      601 ggaaaataaa cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga
      661 tctccaggga tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta
      721 gctgttctta tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct
      781 catggtagag aaaaaacaat caaagaaatg gtaaaaaaaa tgttgaatcg ataatttgaa
      841 cagaaccaaa ttgatgctgt ttgggtgacg gatattactt acattccatg taatgacggt
      901 cgcctctatt tgtccaccta tattgactta gcaactcgta ttctacgtta ctatatggtg
      961 gattcacaca tgaaaaaaga gattgtcgtc cgtccattac aaatttatta aggagaattg
     1021 cctacggtaa ttcattccga tagaggtagc cagcatcggt cttttattta tcaagagctg
     1081 ttgaccgacc accacatcac acattcaatg agccaaactg ggactcctgt tgacaatgca
     1141 gtaattgaag cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa caaatataaa
     1201 agtaaaatag agatgaaggt gttaatccaa gattatttag aagactacta cccaaacaaa
     1261 agaatccata caaaatttat gatgactcct tgacaatttg aagcgaagaa aataa
//
LOCUS       DAZUMI010000252         1311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000252 DAZUMI010000000
VERSION     DAZUMI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.265"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG81C_003009"
     CDS             200..742
                     /locus_tag="ACG81C_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066080.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1311
                     /locus_tag="ACG81C_003010"
     CDS             898..>1311
                     /locus_tag="ACG81C_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5066081.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          532 a          148 c          255 g          376 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAZUMI010000253         1308 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000253 DAZUMI010000000
VERSION     DAZUMI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.266"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..532
                     /locus_tag="ACG81C_003011"
     CDS             35..532
                     /locus_tag="ACG81C_003011"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5066082.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="ACG81C_003012"
     CDS             complement(569..1252)
                     /locus_tag="ACG81C_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5066083.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          451 a          221 c          231 g          405 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaaatag tcctcatttc ttgggagttt tgagacta
//
LOCUS       DAZUMI010000254         1216 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000254 DAZUMI010000000
VERSION     DAZUMI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.267"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG81C_003013"
     CDS             <1..963
                     /locus_tag="ACG81C_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5066084.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          403 a          232 c          248 g          333 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtccta
//
LOCUS       DAZUMI010000255         1203 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000255 DAZUMI010000000
VERSION     DAZUMI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.268"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..672)
                     /locus_tag="ACG81C_003014"
     CDS             complement(4..672)
                     /locus_tag="ACG81C_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5066085.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            806..1093
                     /locus_tag="ACG81C_003015"
     CDS             806..1093
                     /locus_tag="ACG81C_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066086.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          455 a          203 c          205 g          340 t
ORIGIN      
        1 gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt
       61 tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata
      121 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
      181 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
      241 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
      301 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
      361 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
      421 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
      481 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
      541 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
      601 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
      661 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
      721 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
      781 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
      841 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
      901 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
      961 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
     1021 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
     1081 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
     1141 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
     1201 agg
//
LOCUS       DAZUMI010000256         1198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000256 DAZUMI010000000
VERSION     DAZUMI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.269"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG81C_003016"
     CDS             <1..459
                     /locus_tag="ACG81C_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066087.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG81C_003017"
     CDS             473..904
                     /locus_tag="ACG81C_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066088.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG81C_003018"
     CDS             906..1043
                     /locus_tag="ACG81C_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5066089.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          436 a          174 c          252 g          336 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactcttt
//
LOCUS       DAZUMI010000257         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000257 DAZUMI010000000
VERSION     DAZUMI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.270"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG81C_003019"
     CDS             <1..1076
                     /locus_tag="ACG81C_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5066090.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZUMI010000258         1184 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000258 DAZUMI010000000
VERSION     DAZUMI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.271"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..492
                     /locus_tag="ACG81C_003020"
     CDS             82..492
                     /locus_tag="ACG81C_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5066091.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            506..862
                     /locus_tag="ACG81C_003021"
     CDS             506..862
                     /locus_tag="ACG81C_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5066092.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          421 a          166 c          222 g          375 t
ORIGIN      
        1 ttttatattt tattagctaa taattggtat tttaaaagaa attactgtag aattggatta
       61 taattaaaaa ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt
      121 agaaaagcaa gagcatggtt gaaagcgaat caacttgaat ttgaagagaa gaatattttt
      181 gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt
      241 caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa
      301 ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct
      361 attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt
      421 gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc
      481 ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt
      541 aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga
      601 cttagaagaa cgcgatttaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt
      661 gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct
      721 ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag
      781 acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc agttgatgga
      841 acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata
      901 cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat
      961 ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata
     1021 tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt
     1081 aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta
     1141 gaaatgacat gataagataa ctgagttgtt aaaaaagaac cact
//
LOCUS       DAZUMI010000259         1146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000259 DAZUMI010000000
VERSION     DAZUMI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.272"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1061)
                     /locus_tag="ACG81C_003022"
     CDS             complement(<1..1061)
                     /locus_tag="ACG81C_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066093.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          303 a          255 c          182 g          406 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaactttt
     1141 tatact
//
LOCUS       DAZUMI010000260         1126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000260 DAZUMI010000000
VERSION     DAZUMI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.273"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..760)
                     /locus_tag="ACG81C_003023"
     CDS             complement(206..760)
                     /locus_tag="ACG81C_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5066094.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          363 a          218 c          130 g          415 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatacttcc gaaaattcag ttatcttcat taatctcacc ttctaagttt tcattataca
      181 caaaaaatat atcctaatat ctcaattagc ctcccaattt aaaaccacgt aacgatgccc
      241 ctatttcatc ttcagttata ccaatgtacc tttttgtaat tgcttgcgag ctatgattaa
      301 aaataaacat taaggtagca atgtctctag ttttcttgta gtagtgatac ccaaaagttt
      361 ttcttaatgt atgtgttcct atatcatctc ttcctaattt ttctgctaca tctgcatata
      421 tctgatagac acgatccctt gttatatgtc gaccttgttg ccgacttgga aataaccaat
      481 cattttcttc tttaccttca gtatataata aaatctcttc tctcaaatta tctataaaaa
      541 tcggttttcg cttacctgtt ttatgttcaa caataatagg cttactttta cctcgtatgt
      601 ctccaacttt taatcctata atatctgaca tacgtaaccc attattgatt ccaaacaaaa
      661 ataaaaaact atctctttct ggattacttg ttcttcttag ccaaaactga aaatcttgta
      721 tctcttcatt tgtccttaac ggttgtacat tatagctcac tattgtttcc cttcttaaaa
      781 atattctgag ttcatgatta tatatatatt ttaccataat catgaactta tatatatact
      841 ctgttaaaag tgaagaaaat caaggggttt agtttatgat tttatatcat ttcagtaact
      901 cagaatattc cgactttttt gtacaaaaaa attaacctta cttatatgaa gattctaagc
      961 tcttatttaa attttagacg ggcagttatc cgtagaagtt cttagaatag ctaatttaca
     1021 taattaaatt atatgaaatt taagtttgaa caatatacta agaactcccc ttactataaa
     1081 gtgaaataaa ttagcatatg aataaccgtg ctaccaaact tttcat
//
LOCUS       DAZUMI010000261         1098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000261 DAZUMI010000000
VERSION     DAZUMI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.274"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..136)
                     /locus_tag="ACG81C_003024"
     CDS             complement(<1..136)
                     /locus_tag="ACG81C_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5066095.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     Y"
     gene            complement(142..372)
                     /locus_tag="ACG81C_003025"
     CDS             complement(142..372)
                     /locus_tag="ACG81C_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066096.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(631..744)
                     /locus_tag="ACG81C_003026"
                     /pseudo
     CDS             complement(631..744)
                     /locus_tag="ACG81C_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          391 a          175 c          163 g          369 t
ORIGIN      
        1 aatagtagag attaattaat aagctatctt cgattgctaa aaagaaaact tctgaatccc
       61 acaccttata aaaatctttg acaaatctat tcgaaggatc aaatttaaac cataattgcg
      121 tctcattaaa aagcataacc attactccga tctattttta aacttagtct ctacctctaa
      181 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      241 ttgtttattt tcggaaaaag ctaatatagc taaaattgaa acatccatct ggaatttgtc
      301 tttttcgttg ctttgctcat aaaaatctgc atattcatca ctaatatttt ttctaataaa
      361 ttcttccatc ataaagatca cctcgaaaag agtatacgaa caaacgttct ttttgtaaag
      421 gtggaattta ttatactaaa taaataggtg aacaagtact tgtgccaaat tatgtgccaa
      481 aaaaaatcga atttaataaa acacaaacaa aaagaaatcc tattatgata acatttctta
      541 taataacaaa cacaacagaa gacgtgtaat agttagtcag gaacgtacaa ataacccctg
      601 tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct
      661 atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc
      721 atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac
      781 taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat
      841 taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact
      901 ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt
      961 tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat
     1021 ataaaaaaac ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct
     1081 ttattctatg ctttatat
//
LOCUS       DAZUMI010000262         1094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000262 DAZUMI010000000
VERSION     DAZUMI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.275"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..254)
                     /locus_tag="ACG81C_003027"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="ACG81C_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(286..447)
                     /locus_tag="ACG81C_003028"
     CDS             complement(286..447)
                     /locus_tag="ACG81C_003028"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFS5066097.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(771..>1094)
                     /locus_tag="ACG81C_003029"
     CDS             complement(771..>1094)
                     /locus_tag="ACG81C_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066098.1"
                     /translation="REILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKEGFK
                     TPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMAQFR
                     SENVN"
BASE COUNT          310 a          237 c          144 g          403 t
ORIGIN      
        1 gtcccttaat tttgatatag gtttcgtcca ttttccatga atagaaggat tgtctatttt
       61 tcttcttcca aagataatcg aggactttgc tgtactcttg cacccaacga taaatcgtag
      121 tatgacaaac atttattcca cgatcatata acaattcctg aacttcacga tagcttagat
      181 tgtaacgcag gtagtaacta acagcgacaa taatgacgtc attttttgaa ttgtttgcct
      241 ttaaaatgat tcattactct atcctctctg tcttttttct caattttaca ctaaaataga
      301 ttttttggaa aactttgcaa cagaaccact tttcgtaata tcaatgtctt tattcgtatg
      361 atttttcgtt ttacgatcta catgattttg aagtccattt aacgacccaa ctttatgctt
      421 ttgtgttcgt gccaccatat aggccataac tgtgtacttc ctttcgttaa aattattgcg
      481 agaatcacaa gctaaattcg gaaaaacctc catgtaaggt ataacttact agacttatac
      541 tgcgtaaagt ctgtaagctc tgccgagggc tatatcggct taacagccga tgagaaactc
      601 cgtttctctc aaacgaaaaa gcgaacgaaa aacgactgcc ttcgcattcc ttttttacgt
      661 ccttttttcg ttcaaaacca ttggcttttt gcgtcattgt tttcacaatt tttattccgc
      721 aaaaaaccaa aagtgaaaag tattgctttt cacttcaacc cctcctcaat ttagttcaca
      781 ttttctgaac gaaattgtgc cataaattga gtcaaaccct taacatcatt cgtacgccaa
      841 tatgcaccat tttcttgcca aactaatcca tctgcaaatt ttcttagcga tactaaaacc
      901 tcatcattta ctgaatgatt tcttttattt gattccaaaa aatctaatgc ttcagcaggt
      961 gtcttaaagc cttccttttt acaatataat ttcgctaaag aaatcatttc tttctgtcta
     1021 atctgttttc gtttcgctct aatcttcgct tcttttgctt tcaaatccgc catttctttt
     1081 gccaatattt ctct
//
LOCUS       DAZUMI010000263         1088 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000263 DAZUMI010000000
VERSION     DAZUMI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.276"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1088
                     /locus_tag="ACG81C_003030"
     rRNA            <1..>1088
                     /locus_tag="ACG81C_003030"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          287 a          250 c          332 g          219 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagc
//
LOCUS       DAZUMI010000264         1076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000264 DAZUMI010000000
VERSION     DAZUMI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.277"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..747
                     /locus_tag="ACG81C_003031"
     CDS             22..747
                     /locus_tag="ACG81C_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5066099.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            880..>1076
                     /locus_tag="ACG81C_003032"
     CDS             880..>1076
                     /locus_tag="ACG81C_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5066100.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGIN"
BASE COUNT          388 a          126 c          209 g          353 t
ORIGIN      
        1 atcatttaga aggagaaagt tatgttagaa gtgaaaaagc ttaatgtttg ttataacgat
       61 tttttagcat tgaacgagat ttctttaaaa atacaagaag gtgctttaac aggtattatt
      121 ggtccaaatg gtgctggaaa atctacattg cttaaagcaa tgttaaatat tatccctcat
      181 cagggagagg tctccattaa taatgaagat atcaataaaa aactggtgaa gattgcatat
      241 gtagagcaaa aagctgatat tgattttact tttccaataa aagtaaaaga atgtgtatct
      301 atgggaacat atgccggaat gaaagttttt caaagaatta aaaatgcaga atggcaaaga
      361 gtttcaaagg ctttggaaaa ggtcgatatg ggaaaatatt ctaatcatca aataggagaa
      421 ttatctggag gacaatttca acgtgtactt ttagcaagat gtcttgctca aaatgcggat
      481 ttcatatttt tagatgaacc atttgtaggg atagacttag ttagtgaacg aataattatg
      541 gatacgttaa aagaattgaa acatcaggga aaaacagtgc taattgtgca tcatgattta
      601 agcaaggtaa agaagtattt tgataatatt attcttttga atcgaaatct aattgctcac
      661 ggaagtgtag aatctgtatt taatgaagag aatttaaaaa aagcctatgg agatactata
      721 tttattggag aggggggaac ggcttagcct ctcaatccaa taaatatatt atctcaaaag
      781 aaatgagtta ataccaaatt aaaataacga tggttcatag ggttatccaa tcaaaaccgg
      841 aaggttaaaa ccctaactct aatatacaag gggaataata tgcttcaaaa ttttattaat
      901 ggtctgtatg actttcactt tttacagaat gctctaatta catctgtagt tatagggatt
      961 gtttctggaa cagtaggttg ttttattatt ttaagaggaa tgtctcttat gggtgatgct
     1021 atttctcatg ctgttttgcc aggagtagca atttcttata ttttaggtat taattt
//
LOCUS       DAZUMI010000265         1066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000265 DAZUMI010000000
VERSION     DAZUMI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.278"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            445..591
                     /locus_tag="ACG81C_003033"
                     /pseudo
     CDS             445..591
                     /locus_tag="ACG81C_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(679..972)
                     /locus_tag="ACG81C_003034"
     CDS             complement(679..972)
                     /locus_tag="ACG81C_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5066101.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          332 a          173 c          160 g          401 t
ORIGIN      
        1 aaatactctt tttttacgat tattgaaaaa aaaagataaa acttgattaa ttgaagtatt
       61 gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt atctactatt
      121 tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt cgactgcagt
      181 gttgtgtccc tcatcacgaa taaacggtgt tcaattagct gatcttgtat tcacttaaaa
      241 aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca ggacaaaata
      301 ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac gaaatcttgt
      361 tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt tggtatttat
      421 tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga ttttttcatg
      481 tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg ctcattagct
      541 tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata gatacagatg
      601 acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac ttttttcaat
      661 gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc gaccgtctga
      721 acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat aatcgccact
      781 agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca ctccatggtc
      841 aaagcgtttc aagacttctt tcaataggat gtatttcttt tttgttgaac ttggtacttg
      901 ttttactttt ccatcattca tgaatgtgtc taaaactttc atcttttctt gctccgtata
      961 agtataaacc atcttctttc tccttttgat tctaagaata ctccatttat gttagaatat
     1021 tctggatttt ttattaaagc ggttactctt aagaaagata ttatca
//
LOCUS       DAZUMI010000266         1045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000266 DAZUMI010000000
VERSION     DAZUMI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.279"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..945
                     /locus_tag="ACG81C_003035"
     CDS             <1..945
                     /locus_tag="ACG81C_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS5066102.1"
                     /translation="INDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNVAANMKNNY
                     NGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRA
                     YIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKE
                     KLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHV
                     AGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNF
                     PSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          362 a          179 c          212 g          292 t
ORIGIN      
        1 attaatgacg agtataattt agcgccgaat caaggagact ccagattagc aattcctaac
       61 aaaattattt tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg
      121 aaaaataatt ataacggaag caattcttat actacagatg ttattggtga cggtgggatt
      181 gtttaccgtg tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat
      241 gcgcctgtac agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat
      301 cgagcttata ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt
      361 gatcaaggaa cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat
      421 gtttggggga accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa
      481 aagcttgctt atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      541 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      601 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      661 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      721 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      781 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      841 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      901 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      961 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
     1021 atcgcatatc ttcactatca cccat
//
LOCUS       DAZUMI010000267         1043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000267 DAZUMI010000000
VERSION     DAZUMI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.280"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG81C_003036"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG81C_003036"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5066103.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          374 a          132 c          182 g          355 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aac
//
LOCUS       DAZUMI010000268         1006 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000268 DAZUMI010000000
VERSION     DAZUMI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.281"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..>1006
                     /locus_tag="ACG81C_003037"
     CDS             18..>1006
                     /locus_tag="ACG81C_003037"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5066104.1"
                     /translation="MILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPFCFIS
                     KQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLLFDEF
                     RSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVTDMNA
                     PYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQLKSL
                     FKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLLTAYQ
                     EKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLHT"
BASE COUNT          302 a          210 c          191 g          303 t
ORIGIN      
        1 atggcaagaa gatctctatg attctcctga atcgtagcgg taataaacgg acctatcttc
       61 ggttgaagaa acagcgatac ctctgccgag catgtaaaaa atattttacc gctcggacgt
      121 atctcgttac cccattctgc tttatttcta agcagattca ttacaagatc ttagaagaat
      181 tgacagagcg tcaatctata aaagcaattg gtaaacattg cgatgtttct gtgacaactg
      241 ttcagcggac attgtccgcc ttgtcatcag atatccgtca gaatcttgag tatttacctc
      301 ggtgtctatt attcgatgag tttcgttctc tttctacatg gcacggtaaa ttttcttttt
      361 cttgtatgga cggtgagacc gggaaactgt ttgagatctt acctagccgc cgtaaaaaag
      421 atctcgttgc ttttttcatg cgtttttctc tcaaagcgag gttgggtgta cattacatcg
      481 taacggatat gaacgctcct tatttctcac tagttaaaga gtgttttcca aatgcacaag
      541 tgatcgtcga tcggtttcac attgttcaac atctgaaccg cttcttcgat gcggttcgaa
      601 aacgtgtgat gaaaacactt gatcaaaaag acccaataca agctaaacag tatcgccaat
      661 tgaaatcctt attcaaatta cttttaaaat cagaggatcg tttagattac aatacactaa
      721 cgaaaagacg caatttcaat tggagactat tgactgaaac agaagtagtt gaccgccttc
      781 taaagatttc tcctgaactt aaaacagctt atcgttacta ccaagacctg cttactgcgt
      841 atcaggaaaa agatccagat acttttttcc agctgcttcg agcaatgcct ggagaagttc
      901 cattggaact gcaccatatc aaaaaagcct ttttcaaata cgagaaaggg attcggctgg
      961 cattactgaa gaagtattcg aatgcacgac tggaaaatct ccatac
//
LOCUS       DAZUMI010000269         1003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000269 DAZUMI010000000
VERSION     DAZUMI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.282"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG81C_003038"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG81C_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZUMI010000270          982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000270 DAZUMI010000000
VERSION     DAZUMI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.283"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..147
                     /locus_tag="ACG81C_003039"
     CDS             <1..147
                     /locus_tag="ACG81C_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5066105.1"
                     /translation="FYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLM
                     GIPA"
     gene            230..847
                     /locus_tag="ACG81C_003040"
     CDS             230..847
                     /locus_tag="ACG81C_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066106.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
BASE COUNT          334 a          122 c          183 g          343 t
ORIGIN      
        1 ttttatcgaa gtctccggac tgcctcatcc acgattaagg ggatggagac cattcgagga
       61 atatataaaa agaaccgaag aaatggaacg ctcttcggct tttcggtgtc tactgagatc
      121 aaggtattaa tgggaatacc agcctaagat atttggagtt cagagagggc gcgtttgatt
      181 ttcaaacttc gcaacagaac cattatttta aagtgggagg aaaattgttt tgtggatatt
      241 taaacaagaa atctttgcta aattatttag tttagatgga ttaggttggg gaatcttatt
      301 ttttattttt ttatttttag gatctatgtg gatctggttt gttgataaaa aaaattctta
      361 tgagaaagta atagtgaaaa aaatgaatcg aaatgaattc gatgattttt caaaggaaca
      421 tagagttgaa ttgtacgata gtcatgaaat taacagtact tctccatctt attattgggt
      481 atttggttgc gaagaaaaac cattggagat taaattgttg aaaatgcttg aaaatcctcc
      541 agtcattgaa gatttttctg caaaaaacgt tgaaaaacag atgtttgaac aggtacaaat
      601 aagaggtcaa aacaaaattt cattagaacc agatgaatat gcagcctttt ttttagaaac
      661 aaattcaggt acttcaatgc ctgttttcaa agttaaatta aaaatgaatg gtcgtttatg
      721 cacttatgat cttgttgcaa atatgcgcta tggttctact ggtgatagac gtactatttt
      781 aaaatttgag caaaccttta gaagtttttt gaaaaataga gtatcccaaa tttttaattt
      841 ggattgaaaa aattaaaaat ttggccaaaa atactacagt tttttagagc aatcatctta
      901 tgatgattgc tctttttttg ttgtttttgt gtcattcgtt gttcaaatcc tctattcgta
      961 aaataaatat tttaaactag aa
//
LOCUS       DAZUMI010000271          972 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000271 DAZUMI010000000
VERSION     DAZUMI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.284"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG81C_003041"
     CDS             <1..963
                     /locus_tag="ACG81C_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5066107.1"
                     /translation="PINTFAYTINNEFNLGPNEGSSQVANNQYILLHETANETATGRN
                     EAQYMKRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          337 a          186 c          199 g          250 t
ORIGIN      
        1 cccattaata cgtttgctta tacgatcaac aatgaattta atttgggccc aaacgaaggt
       61 agctctcaag tagcaaataa tcagtacatt ttactgcatg agacagctaa cgaaacagca
      121 acagggcgca atgaagcgca gtatatgaaa cgttcatgga ctagtgctta cactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc taacagtccc gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tagctagaga ttcagcaatg
      361 aaatatggta ttccacttac actagacaca ccgtataatc aaccagggat caaatcgcat
      421 ttatgggtaa cacaaaatat ttggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcctat gatttggctc atggatttac cgatgaaaat
      541 ccgacaactt cagatgataa accagtcatt gatccaactc gagcaggtgc tgcaaatcct
      601 accctgacag atggaacaaa ttacgcccac attgatcagt tcggagaaat cgaaaacgca
      661 aacttacatg tggctggttg gcacatcgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaaaagctg atggaatata tagaccagat
      781 gtaaatcaag cttataatac tttaggaaat gttggctatc atgtatcttt taacatgcgc
      841 aacttcccta ataagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agaagggaac
      901 actaaaggcg gagcacaaga ttttcatgat aaacgctggt atttaaatat tccgcaacga
      961 taaaaatagc cc
//
LOCUS       DAZUMI010000272          972 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000272 DAZUMI010000000
VERSION     DAZUMI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.285"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>972
                     /locus_tag="ACG81C_003042"
     CDS             <1..>972
                     /locus_tag="ACG81C_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066108.1"
                     /translation="FYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLY
                     PLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRS
                     VEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTM
                     VMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVD
                     ILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGII
                     SILLCTKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQ"
BASE COUNT          301 a          143 c          130 g          398 t
ORIGIN      
        1 cttttatgat acggcatttc acattaatcg tgcgttaagt ttagaatcta tttttagctc
       61 ccctattaat tttgaaacgt ttagatcata tggcatgcaa gtaaataatt tttacccatg
      121 gttaactcta tatcctttat ttttattgat caagtttact aatttagcaa taggctataa
      181 cctttttctt tatattgtta cgctcattac tctatttata tgtcattatg taatgtatga
      241 aattaccaaa aaacatgtta ctagctcctt tttcgctatt atatatacaa catcttcttt
      301 taggagtgta gaaatatttc ttcgaggggc aatgggggaa ttactcgcaa tgtcaatact
      361 tcctcttatt ttactcgggt ttataaagct ttacgactca aaaaaagaat cttgggttat
      421 gttggcgatt agcatgactt tattaattta cactcatgtt ctttctgtag ctatgactat
      481 ggtaatgatt ataattgctt taattattca attttatcgg aaaaagaaaa tagaaatctt
      541 cttaattatt aaattaataa aagctgctat agttacttta cttctttcac tagcgtttat
      601 aggtcctatg attgagcaga cattgtatca agatctgaat agaccttatg tggatatatt
      661 acaaaaaaga gcaatttatc tgggtaaaga gtttttgatt ggaagtatag attcagaatt
      721 actatcattt ggtattggct taactttgct tatttgttta attatactaa catttaactt
      781 taaaaaatta ggaatcttat ctaaaatcac ttatataatg ggtataatat ctattctact
      841 ttgtacaaaa atatttccat ggtttgcctt acaaaatact cctttaaata ttattcaagc
      901 tccttggcgt tttatgatat tttcaacatt gttcatatct ttttcagtat ctctatttat
      961 ttctccacag tt
//
LOCUS       DAZUMI010000273          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000273 DAZUMI010000000
VERSION     DAZUMI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.286"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG81C_003043"
     rRNA            <1..>943
                     /locus_tag="ACG81C_003043"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUMI010000274          906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000274 DAZUMI010000000
VERSION     DAZUMI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.287"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..720
                     /locus_tag="ACG81C_003044"
     CDS             <1..720
                     /locus_tag="ACG81C_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5066109.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          339 a          143 c          178 g          246 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
      901 cattgg
//
LOCUS       DAZUMI010000275          904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000275 DAZUMI010000000
VERSION     DAZUMI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.288"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>904)
                     /locus_tag="ACG81C_003045"
     CDS             complement(37..>904)
                     /locus_tag="ACG81C_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066110.1"
                     /translation="KKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEK
                     SVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIR
                     RELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKF
                     FLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGK
                     PEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQI
                     DDFPEEIIDRIE"
BASE COUNT          291 a          156 c          139 g          318 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 cttt
//
LOCUS       DAZUMI010000276          877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000276 DAZUMI010000000
VERSION     DAZUMI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.289"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..245
                     /locus_tag="ACG81C_003046"
     CDS             <1..245
                     /locus_tag="ACG81C_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066111.1"
                     /translation="LNLKMFIIEKWIVIIAKIFSSISIIIPMTLTFSKEQFNVATTFV
                     SFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(304..474)
                     /locus_tag="ACG81C_003047"
     CDS             complement(304..474)
                     /locus_tag="ACG81C_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066112.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(474..773)
                     /locus_tag="ACG81C_003048"
     CDS             complement(474..773)
                     /locus_tag="ACG81C_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066113.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
BASE COUNT          257 a          175 c          131 g          314 t
ORIGIN      
        1 tgcttaattt aaaaatgttt ataattgaaa aatggatagt tattatcgca aagattttct
       61 catcgatatc aattatcatt ccaatgacat tgacgttttc taaagagcaa tttaacgtag
      121 ctactacatt cgtatctttt atagctctat tttcgtttgc tgttcctaaa acttttcaaa
      181 gcaaatctag caacgcaaag gttaatgtag aaaacactga tagaaaaaat gatgaaagtt
      241 attaaaaaaa ataattcttt catttattgt caaacctttc ttatagacat tagacttttt
      301 tagtcagtcc ctcccgactg gcttttttgt tttgtactca gcttcatcca gccctataaa
      361 aatccaaacc atgtacacaa tcgtacctat caacgcttgt ctgcttcccc agaggcctaa
      421 agcgtagatg attagcggtg cgctgaatac tagtgctctg ttgaatttgc ccattaactt
      481 gcctccttca aaacatctga aggcgataaa ctttttacac gatatttgtt tgcgtctttc
      541 cactttaaaa accaaataaa agtatggtaa tgaataaatg tggttgagtg acctggacga
      601 agtattcctt ctgcaaattc aggaattgtt tccatttcct tgcagtattc aactaaagta
      661 gtttttgaca tgccgtggaa cttctccaag attaaacttt gtcggtacca gtcgtctggt
      721 tttactgcct tctcagcagc ttctatcagc tctgaaagtg ttggtttttt cacttattat
      781 ccctcctaaa aattatcgtt ccatactcga tgtttaacat ttcttgaagc tctgattttg
      841 taagaatgat tttatcttcc aacgacttta cctccct
//
LOCUS       DAZUMI010000277          838 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000277 DAZUMI010000000
VERSION     DAZUMI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.290"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..556
                     /locus_tag="ACG81C_003049"
     CDS             <1..556
                     /locus_tag="ACG81C_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5066114.1"
                     /translation="NIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAFKS
                     MGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIH
                     LDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFEN
                     VVTGKENWNDLYHANEVLKIAEEK"
     gene            557..>838
                     /locus_tag="ACG81C_003050"
     CDS             557..>838
                     /locus_tag="ACG81C_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5066115.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDS"
BASE COUNT          321 a           90 c          156 g          271 t
ORIGIN      
        1 aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc
       61 tatttgttct acttatcttc ctgattggag aaaaggtact gattatagaa ataattattc
      121 tgcctttaaa tctatggggg gaggagtaga attagactgt atacatgaat tggattatat
      181 tataaaccta tttggtttcc ctaaatcagt aaaaaaacag tttggtaaat attcgaatct
      241 tgaaattaac agtaatgatt tagcaacata tatattgaga tacgatgata aaattataga
      301 aattcattta gattattttg gaagagttcc tacaagaaaa tttcaattgt ttactttaga
      361 tgaatgtatc aatgttgact tattaaataa taaaataagt tttgaagaaa acattgttaa
      421 agaaatgcct gaatctgcta atgatatgta tgagaaggag ttgaaatatt tctttgaaaa
      481 cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag tattgaaaat
      541 agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg ggtctaaggg
      601 gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt tcgctttagc
      661 aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt gtttgaatac
      721 ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga aaattgtaga
      781 acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac gtgattca
//
LOCUS       DAZUMI010000278          832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000278 DAZUMI010000000
VERSION     DAZUMI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.291"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>832
                     /locus_tag="ACG81C_003051"
     CDS             <1..>832
                     /locus_tag="ACG81C_003051"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5066116.1"
                     /translation="ELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQEYDKLIL
                     ATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYIGVEIAEA
                     IRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEK
                     IVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKDIYAIGDC
                     ATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFNYNFYAAG
                     L"
BASE COUNT          317 a          106 c          144 g          265 t
ORIGIN      
        1 agaactaaat tctaagataa aaaagttgtt tttggagaca gcagttgaca aaattgatat
       61 tcaaaataaa aagattattt gtgtaaaaaa agatggcaca agattttccc aagaatatga
      121 caaacttata ctagctacag gttcaaagca aattatgtta gatgtaccgg ggaataattt
      181 agctaatatt ttttcgataa agaattttcc aaatgctaaa gagattttta aagctttaga
      241 tgataatact attaaaaaag taggaattat tggagcagga tatattggag ttgaaattgc
      301 tgaagccata agaaaaagag ggaaagaagt atatcttttc gatgttgcag atagagtgtt
      361 gtcgacttat tacgaccgta gtttttcaga taaagttgaa gaaatactat caaaaaatgg
      421 aatacatctt tgtctatcag aaagtcctaa aaaatattta ggaagaaaaa agattgaaaa
      481 aatagtcact acaagtggta cccaatactc tatggatatg gtcgtacaag ctataggatt
      541 tctatctaat agtccaattg gcaaaaagga actcttgaga gatgttagtg gagcatatct
      601 aactaatgaa taccagcaaa caaatataaa agatatatat gctattgggg attgtgctac
      661 aacttttttt acgtccatta atagaagtag tgttgatttt tctatttcta atgctctaag
      721 aacagcttat attgcaactc aacatattga caatcaagat tttacacctt ctggttccca
      781 atttacaaat ggcttttcta tatttaatta caatttttac gcagcaggtt tg
//
LOCUS       DAZUMI010000279          818 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000279 DAZUMI010000000
VERSION     DAZUMI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.292"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(478..702)
                     /locus_tag="ACG81C_003052"
     CDS             complement(478..702)
                     /locus_tag="ACG81C_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066117.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          296 a          114 c          150 g          258 t
ORIGIN      
        1 tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt gggttgagag aaatctaatt
       61 gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt gtgggtacta
      121 tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc tatcaggttt
      181 gatggaacga agacaacctc ccgacatcaa gtcaaaagcc acttgagatt ttgatctgaa
      241 aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc gtaatcaatg
      301 ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac agtacctctt
      361 tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt gttgttatac
      421 cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct gtgaatctta
      481 ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata aaaatcctgt
      541 ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca tagccatata
      601 aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc gaaaagtttt
      661 ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt catagcctcc
      721 ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta cgttttttag
      781 aataaaaatt ataagggata agtaggctgc tgtctata
//
LOCUS       DAZUMI010000280          813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000280 DAZUMI010000000
VERSION     DAZUMI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.293"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..542)
                     /locus_tag="ACG81C_003053"
     CDS             complement(144..542)
                     /locus_tag="ACG81C_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5066118.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(539..775)
                     /locus_tag="ACG81C_003054"
     CDS             complement(539..775)
                     /locus_tag="ACG81C_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5066119.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          255 a          142 c          118 g          298 t
ORIGIN      
        1 tccttttaaa tcaattattt caaaattaat ccatattttc catattttta tgaaaaaact
       61 attttgtatg attaaacata aaaaaatcag catattcctt aatgacatgc tgatctaatt
      121 gagaaataaa gtttttaatt cgttcatttt attgcggttt gtcgtaaata actggttatc
      181 ttggacttta gtgaatcgaa atccaatgaa ctagtggtaa tcattatgat aaaatcaaca
      241 gcttcttgac gagaaaaagc aactggacaa ttattcaatt gaagaaacaa caacatggca
      301 acaagagcag ttcgtttatt gccattatga aaaggatgtt ttttagctaa attaattgct
      361 aaaatcgccg ctttttcata gatttcagga tagagttctt gattgaatac tgcttgtgaa
      421 ggctgtttga cggccatatc taatgcattt gcatctttta caccgatcat ttcgcctggc
      481 gagtgacgta aaataacttt cgcgttgatt ttgataattt ctttagtcga aagatacctc
      541 ataaatcaac caaatctttt aacgcgtctt catattgatt catgatcgaa tcaactaatg
      601 aatcaatatc aggttcttct tgtgctttga tcattcgtac agaaccatca gattgaaaaa
      661 cataactgat tgcgtcacct gtctgcacac ccatgaattc ttttacttct ggtgggatag
      721 ttgttacatc gctattgcca gattttctaa ttttatactt tttcattttt ttcatatttc
      781 gccaccctct ataccattct cattctatag taa
//
LOCUS       DAZUMI010000281          796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000281 DAZUMI010000000
VERSION     DAZUMI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.294"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..>796)
                     /locus_tag="ACG81C_003055"
     CDS             complement(24..>796)
                     /locus_tag="ACG81C_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5066120.1"
                     /translation="YITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKW
                     MVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSS
                     DNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRA
                     HGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSI
                     FIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          305 a          141 c           97 g          253 t
ORIGIN      
        1 aagcaatgaa agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt
       61 cccaaataat accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc
      121 tccaccattt catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca
      181 taattcactt tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa
      241 gcatagatac atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta
      301 tgcatgtttc catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt
      361 ttcaaaaatt ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa
      421 gttggtctta taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg
      481 gagttatcac tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat
      541 tcatttgact ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa
      601 tccttagcat aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc
      661 atccatttga tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca
      721 aacaacaaaa gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa
      781 gaaagtgtaa tataaa
//
LOCUS       DAZUMI010000282          788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000282 DAZUMI010000000
VERSION     DAZUMI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.295"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..717
                     /locus_tag="ACG81C_003056"
     CDS             1..717
                     /locus_tag="ACG81C_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5066121.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          196 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttat
//
LOCUS       DAZUMI010000283          770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000283 DAZUMI010000000
VERSION     DAZUMI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.297"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="ACG81C_003057"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="ACG81C_003057"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          250 a          126 c          150 g          244 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta
//
LOCUS       DAZUMI010000284          744 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000284 DAZUMI010000000
VERSION     DAZUMI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.298"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..>744
                     /locus_tag="ACG81C_003058"
     CDS             145..>744
                     /locus_tag="ACG81C_003058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066122.1"
                     /translation="MVLKQGKQDYQIIGYLEDDLNKIKINSDDVMYFFMTPLSDFPSS
                     VIDNVEGIQIYSNTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQAR
                     NYYQGDDLELIPKPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGK
                     LLGKIINFSKDNEDYPLNVFWENTDGSRVLFGEIIGQNNS"
BASE COUNT          309 a           93 c          109 g          233 t
ORIGIN      
        1 aagaaaatga tattaatgaa ttatggatta caacctttca attaattccc agaatgaaaa
       61 aaaagattat acaaagttta aaaaataaaa taaaaattca agaattaaat gattttacta
      121 tgcttatgaa ggaggaaaat ataaatggtt ttaaaacaag gtaaacaaga ttaccaaatt
      181 attggttatt tggaggatga tttaaataaa attaaaataa actcggatga tgtaatgtac
      241 ttttttatga ctcctctttc agatttcccc agtagtgtga ttgataatgt agagggtata
      301 caaatatata gtaatacgct tactaaattt gggtttattg atgatataaa cgaatctcat
      361 agtactcgtg cccaatcact taaagaattt ttagatcctt atattatcgt ttgtacacca
      421 aaaattaaaa gaaatcatga acaagcaaga aattactatc aaggtgatga tttagaatta
      481 atccccaaac caaagtctga agtttttaaa aatttaataa aagagaatga atcattagta
      541 cctataccgg tcttttctat aaataacagc gattctgtaa tcaaagacga gacaagtttt
      601 gaaaaccaat tactaaacgg caaactactc ggaaaaatta taaatttctc aaaagacaat
      661 gaagattacc ctttaaatgt tttttgggag aacactgatg gatcaagagt tttatttggt
      721 gaaataatcg gacaaaacaa ttca
//
LOCUS       DAZUMI010000285          743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000285 DAZUMI010000000
VERSION     DAZUMI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.299"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..>743
                     /locus_tag="ACG81C_003059"
     CDS             177..>743
                     /locus_tag="ACG81C_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5066123.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKN"
BASE COUNT          258 a          107 c          121 g          257 t
ORIGIN      
        1 taagattttt attagaatta cgcctaattt gtgtctctca gggcaattct aagaacgttt
       61 caagagatag cgacactatc gttatcgaaa aaggcgaaaa cctatctcat tttctgctaa
      121 tgtaagttgg tttagctttt gttaatatga ctttatgaaa atggaggtaa ttaaatatga
      181 tttttcctaa agttaatgaa gcaagcttga ataaaggatc cttttttcat atctatgaaa
      241 acgaagattt tgaactgtat aatatccttt cacaaaaaga ggtcatcgaa aaccaaataa
      301 tctgccatcc aattatttac aaatctattt tcaagtatgc attttttgct tccaatgatt
      361 ttcaaagaat ggagattacc gactgtatat tcgaaaactg tgatttttca ggctgtatat
      421 tgacaaaaag catgttgcat cgcgtgaaat ttgtcaattg taaattaaca ggtactaaat
      481 tgatggaaag ttatattggg aacacgttat tcgagaattg taaaatggac tatgtaaatt
      541 tatctggttc aaacataaaa gaaagtacct ttgagcactc tgtcttatca agcgcagatt
      601 ttgtagactg ttccctcact aaaataatgt tttctactaa tgatttagaa aatgctagct
      661 tttttgatac tgatttaaaa aatatcgatt taagtagcaa ctcctttgaa atgattgaag
      721 tacgctcgga acatctaaaa aat
//
LOCUS       DAZUMI010000286          733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000286 DAZUMI010000000
VERSION     DAZUMI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.300"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..>733)
                     /locus_tag="ACG81C_003060"
     CDS             complement(96..>733)
                     /locus_tag="ACG81C_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066124.1"
                     /translation="STTKFANSFWFLENINETVEKNGVYKGDFHFQNAETIEREHHYF
                     DLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNP
                     ENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGED
                     KEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          233 a          132 c           71 g          297 t
ORIGIN      
        1 aaaatatatc ccactcaaaa tcttgacaaa agaaaaaagt tgtgatttcc aaaatcacaa
       61 cttttttctt ttacaataaa tcaatcctta ttaaattatt taggaaaata ttgttcaatt
      121 atttctttat atattttctc atagtatgtt tctgtccatc ctaatgttcc tataaaatca
      181 agtcctctac attttaaagg ttcacttaaa ctattaatat agttaataat gttgtctatc
      241 cttttctctt tatcttcacc ttcaatctgg ggagcatata gtccaatttc ctcccaataa
      301 tatgaatctc tttcaattcc catgatggta gctttggcta atttcaaatc aaataacgat
      361 tttagaattc ttagtgtctc aataaagtct tctctcttca actctttaat ccattgtgaa
      421 ttttctggat tatctaaaat ttcaagttca tcttcattat aagtatattt caaatttgac
      481 ttgcttaact catagataaa tgaagtttct tgactcataa ttcttttaag tacttgacta
      541 taagtttcgt ttgactttgc ccatttcgct aattctacca ctgtattcac taataaatcg
      601 aaataatgat gttctcgttc aatagtctca gcattttgaa aatgaaaatc acctttataa
      661 actccattct tttccactgt ttcattaata ttttctaaaa accagaatga gtttgcaaat
      721 ttagtcgtac ttt
//
LOCUS       DAZUMI010000287          695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000287 DAZUMI010000000
VERSION     DAZUMI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.301"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG81C_003061"
     CDS             complement(<1..446)
                     /locus_tag="ACG81C_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5066125.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          207 a          118 c           93 g          277 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgtt
//
LOCUS       DAZUMI010000288          643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000288 DAZUMI010000000
VERSION     DAZUMI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.303"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          248 a           82 c           81 g          232 t
ORIGIN      
        1 agcaatatat aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga
       61 gcaaatataa aatttaataa aaataagaac taaataggaa aacgtagacg taattttcta
      121 cgttttcctt ttcatatata ttaaatatta cggaaaatta tataaactaa tttagtgttt
      181 tgtttgtaat tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatt
      241 agacattcta tgctcatttt tctccaaatt tgtttcaact ctttcgtact taattttgcg
      301 tatttgaaaa ttcttctttt agagtacttt tattacgata ctagtatgat atatatctac
      361 agcaaacagt taaaatcttc ctaaaataag cgaatagcat tcctaaaacc tataaaattt
      421 ttagatgaag cgacaagtgg acaattaata aactacttga acatgaaatt atggatcacc
      481 tttcaaattt agaaaatgga attcttatac ttattgcgca acatttattg attgcaaaat
      541 tatgtgacaa aaagaactag tagaagaagg aataatggct tgataataaa gtactaccta
      601 ttaaaggtag tactttattt aatttagtat aaattaacat act
//
LOCUS       DAZUMI010000289          637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000289 DAZUMI010000000
VERSION     DAZUMI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.304"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..614
                     /locus_tag="ACG81C_003062"
     CDS             384..614
                     /locus_tag="ACG81C_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066126.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          227 a          105 c          105 g          200 t
ORIGIN      
        1 cgtataaaca gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
       61 tgaaaagcaa tttccgtgaa tttcttctta tttcttggag ttaaacgctt ctgttccagc
      121 ctctttttat ctgtctttca ctaatgatca gttgtagata agcaagttgc tttttcttac
      181 atcataccgc ccatcaagac aaacgttttt agcttgtacg tactggacaa aaatcccatt
      241 atatcaagaa aaaacaacgt tgataatact taaaattatt aggctagaac ggactagtaa
      301 gagttttttg cccctttttt gcccctcaaa tatcgcttta cagccagaac ataagttcgt
      361 ataatcgttt tgaggtgatt tttatgatgg aagactttat tagaaaaaat atcagtgatg
      421 aatatgcaga cttttatgag caaagcagca aaaaagataa attccagatg gatgtttcaa
      481 ttttagctat attagctttt tccgaaaata aacaacctgt aactgcaaaa aaagaaacag
      541 tattctctga aggcaaaata aaaactcgat atatattaga ggtagaaact aagtttaaaa
      601 atagatcgga gtaatggcta tgctttttaa tgagacg
//
LOCUS       DAZUMI010000290          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000290 DAZUMI010000000
VERSION     DAZUMI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.305"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..525)
                     /locus_tag="ACG81C_003063"
     CDS             complement(226..525)
                     /locus_tag="ACG81C_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5066127.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          211 a          113 c           72 g          240 t
ORIGIN      
        1 tgcaacatta ataaataacg atagtttcat aaaatacact tttaattaac attattagtt
       61 tatactagct gtttttgtgt taattaaagg ataaaataca atataaagaa acatctgttt
      121 ctttatttag aataaaaaaa tacagactct gttcaaataa atgaacctag cctgcattat
      181 gagattgact atacttttta attcattgag atgaataact cgtttttata aatgcccaaa
      241 agtaatcggt gccgaagcca ttcctcgacc taattctttt tcaaattttc ctattgttga
      301 tatctccata taaaacggtg atactggttt agacaattct cccctgattg ctaacggacg
      361 taaactacgt tccaaattat ttatgacttt aggataatac tgaccttttt ctaagcctgt
      421 ctttgcgtct atcaaaagta tacgttcctt ttctttgatc tctggattta aaacgagatc
      481 atataatttt agtaccatgt tttctatttc ttcactttta gacatcacga tttcctccca
      541 aaaatatatt tcttcccact acattctata aacaaagtat acgctacatc tagatttttt
      601 taaatcatta ctggattgtt aaaacctttc aataca
//
LOCUS       DAZUMI010000291          597 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000291 DAZUMI010000000
VERSION     DAZUMI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.307"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG81C_003064"
     CDS             <1..435
                     /locus_tag="ACG81C_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5066128.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          193 a          134 c          112 g          158 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatc
//
LOCUS       DAZUMI010000292          579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000292 DAZUMI010000000
VERSION     DAZUMI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.308"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..552
                     /gene="spxA"
                     /locus_tag="ACG81C_003065"
     CDS             154..552
                     /gene="spxA"
                     /locus_tag="ACG81C_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5066129.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          204 a           91 c          111 g          173 t
ORIGIN      
        1 tattaacctt agataacttt cattttttta aaaaaattat gaaaaaaagg tagctattta
       61 aaagttgatt atgtataata gaaacagatg ggagattatt atgatcttct gttacaaaaa
      121 agtgattctt atcacgaaaa ggagtggtgc acgttgttaa cattatatac ttccccaagt
      181 tgtacttcct gcagaaaggc tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa
      241 cgtaatattt tttcagaacc attgaacagt tctgaattaa aagcgatttt acaaatgaca
      301 gaagacggaa cagaagaaat catctcgaca cgttccaaag tattccaaaa actgaacatg
      361 gatttagatg atctgccgtt acaagaatta ttggaacttg tgcaaaacaa tcctggattg
      421 cttcgccgtc caatcatgat tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa
      481 attcgtcggt tcctaccgcg cgatgtacga caattagaat tacgtcaagc acaattgatg
      541 gctggattat agaaggataa tacgaaactt ggaaaagac
//
LOCUS       DAZUMI010000293          571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000293 DAZUMI010000000
VERSION     DAZUMI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.309"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          223 t
ORIGIN      
        1 tcttgatgtg atatccttgt cttttttgta atgtttttaa cttattgctt ttgcaaaatt
       61 tatttacctg ttttctggat caacgatagt gtacattatg ctatccttgt ttggatttta
      121 ggacgtttaa agaggcactt ttttacataa cttaatccat agacctttta agcatgctgt
      181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtatta
      241 cgctttaaac tcttttatat ttgaattcac taagaagcat accatatttt tgaaaaaaag
      301 tgagaaaaaa ggcttataat taggatttag agtaattaat tagatgttta ttttatagga
      361 ctgatactat agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg
      421 tgaataagat aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac
      481 ctttctggat caacgaaagt atactttata tagtcataac gccgatggta actatgcact
      541 gtaacgataa tgctgctaaa atatcttttt a
//
LOCUS       DAZUMI010000294          569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000294 DAZUMI010000000
VERSION     DAZUMI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.310"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACG81C_003066"
     rRNA            <1..>569
                     /locus_tag="ACG81C_003066"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAZUMI010000295          534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000295 DAZUMI010000000
VERSION     DAZUMI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.312"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG81C_003067"
     CDS             <1..>534
                     /locus_tag="ACG81C_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5066130.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZUMI010000296          514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000296 DAZUMI010000000
VERSION     DAZUMI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.313"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>514)
                     /locus_tag="ACG81C_003068"
     CDS             complement(107..>514)
                     /locus_tag="ACG81C_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS5066131.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DAZUMI010000297          509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000297 DAZUMI010000000
VERSION     DAZUMI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.314"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..>509
                     /locus_tag="ACG81C_003069"
     CDS             13..>509
                     /locus_tag="ACG81C_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS5066132.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          183 a           93 c           85 g          148 t
ORIGIN      
        1 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
       61 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      121 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      181 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      241 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      301 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      361 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      421 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      481 tatgtcgcta tttttatgga cgctactta
//
LOCUS       DAZUMI010000298          508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000298 DAZUMI010000000
VERSION     DAZUMI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.315"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DAZUMI010000299          477 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000299 DAZUMI010000000
VERSION     DAZUMI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.317"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..>477
                     /locus_tag="ACG81C_003070"
     CDS             126..>477
                     /locus_tag="ACG81C_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5066133.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          180 a           79 c           86 g          132 t
ORIGIN      
        1 cccgcactat gccattacat ttatatctac gcgaattgta aaattaagtt agaaaaataa
       61 aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa ggagtgtaac
      121 cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa tcatattatc
      181 ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata caaactattt
      241 ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg caccagtata
      301 aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat gaagtagatt
      361 acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg cgaaaagaaa
      421 ggtctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga atatttg
//
LOCUS       DAZUMI010000300          415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000300 DAZUMI010000000
VERSION     DAZUMI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.318"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>415
                     /locus_tag="ACG81C_003071"
     CDS             <1..>415
                     /locus_tag="ACG81C_003071"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5066134.1"
                     /translation="LEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFL
                     GERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFV
                     SLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTE"
BASE COUNT          165 a           49 c           79 g          122 t
ORIGIN      
        1 gttagagatt tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg
       61 tgaagggtca cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag
      121 agtaaaattt ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt
      181 tgtaatgcct tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa
      241 attaaaatta gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga
      301 ttttgtatca ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta
      361 tgctaataaa ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaa
//
LOCUS       DAZUMI010000301          414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000301 DAZUMI010000000
VERSION     DAZUMI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.319"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..414
                     /locus_tag="ACG81C_003072"
                     /pseudo
     CDS             1..414
                     /locus_tag="ACG81C_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          169 a           72 c           76 g           97 t
ORIGIN      
        1 tacgtaaaag aaaccagcaa ttagaaatgg aaaatgatat tttaaagcaa gcagcgctga
       61 tattcggacg aagagacaag taatcgatgc gaataagcat ctttacccta tatcagcgat
      121 gtgcagaata ttaggtctat cacgtcagtc ctattattat caatcaaaac caaagaaaga
      181 cgaatcagaa cttgaagaag tagtcgctga agaatttatc cgcagccgaa aggcctacgg
      241 ctcaagaaaa ataaaaaaag ccttatcaaa acgaggcatt cagatcagcc gacgaaaaat
      301 tagtagaatc atgaaaaata gaggattaaa atcgagctat actgttgctt attttaaagt
      361 acatcattct acttgcaatg aagccaaaac gacaaacgta ttgaatcgta aatt
//
LOCUS       DAZUMI010000302          392 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000302 DAZUMI010000000
VERSION     DAZUMI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.322"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..>392
                     /locus_tag="ACG81C_003073"
     CDS             130..>392
                     /locus_tag="ACG81C_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5066135.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLER"
BASE COUNT          145 a           63 c           56 g          128 t
ORIGIN      
        1 attttttctt tttttcatgt attcttttat ttttataact agtgtattac ttcaaaattt
       61 gatattatat ggcatgaaca gaattaaaat atcagttaaa ataaaagtta aggagcattg
      121 tgtatgacaa ttaaaactag tctccaaaat caagcttacc aatctattcg ccgacaaatt
      181 atctatgctg acttagaacc tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt
      241 tctattggtc gtacaccaat acgtgaagca ctgattcaac tccgacaaca aggtcttgtt
      301 gatacaattc ctcaaagcgg aaattatatc tcattaattg atttagattc ggccaaaaat
      361 gctcgatttg tcagagaaca tttggaacga ca
//
LOCUS       DAZUMI010000303          367 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000303 DAZUMI010000000
VERSION     DAZUMI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.324"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>367
                     /locus_tag="ACG81C_003074"
     CDS             <1..>367
                     /locus_tag="ACG81C_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS5066136.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DAZUMI010000304          336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000304 DAZUMI010000000
VERSION     DAZUMI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.326"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          126 a           54 c           59 g           97 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaataa
//
LOCUS       DAZUMI010000305          330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000305 DAZUMI010000000
VERSION     DAZUMI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.327"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..265
                     /locus_tag="ACG81C_003075"
     CDS             41..265
                     /locus_tag="ACG81C_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS5066137.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          168 a           37 c           69 g           56 t
ORIGIN      
        1 attttgcgta catatgatta aagaaaggag agtgtgacac atggaaacag aatggcaaag
       61 acgtgaaaga aaaaaacaga aaaaagaaga gatgttacga ttatgtcgat tattagatgt
      121 aaaagactgg gacaaaaacg agcaaattgc acaaaaagta cgatcaatca tccataaaga
      181 ggaaaaagtt aaaaataaag acacgcgtag aactgaaaat gaaaagagaa aagtacaaaa
      241 gaaaagagaa aaatcaaaaa gctagaaaga aaaagaggct gagacagaag cgtttaactc
      301 caagaaataa gctggaattc acaaaaattt
//
LOCUS       DAZUMI010000306          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000306 DAZUMI010000000
VERSION     DAZUMI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.328"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG81C_003076"
     CDS             complement(<1..>321)
                     /locus_tag="ACG81C_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066138.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZUMI010000307          317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000307 DAZUMI010000000
VERSION     DAZUMI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.329"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..>317
                     /locus_tag="ACG81C_003077"
     CDS             64..>317
                     /locus_tag="ACG81C_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066139.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESS"
BASE COUNT          140 a           46 c           51 g           80 t
ORIGIN      
        1 aaagccttcg ggctttttct tttaacaatt aataggaacg tatattcgaa aggaatagaa
       61 ataatgtggt tagaaaaaat aaatgacaaa aaatttaagt acaccgaacg ttatacggat
      121 cccttaactg aaaaaaagag aaaagtgtcc gttactttat ctagcaattc acgccaagct
      181 tggaatcaag ctaacttact tttgaatgaa aaaatcgcag aaaaaataaa acgaaatgaa
      241 gaattaccac taacttttgg agaattaaaa actaaatggg atgaaaaata caagccaact
      301 gtaaaagaaa gttccta
//
LOCUS       DAZUMI010000308          302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000308 DAZUMI010000000
VERSION     DAZUMI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.333"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..256)
                     /locus_tag="ACG81C_003078"
     CDS             complement(32..256)
                     /locus_tag="ACG81C_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5066140.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           82 a           72 c           48 g          100 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt attcattttc ttctctccct aaacaaagtt ttgatttgtt gcgtgtgttc
      301 ca
//
LOCUS       DAZUMI010000309          298 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000309 DAZUMI010000000
VERSION     DAZUMI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.335"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          105 a           53 c           43 g           97 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaatc
//
LOCUS       DAZUMI010000310          293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000310 DAZUMI010000000
VERSION     DAZUMI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.336"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>293)
                     /locus_tag="ACG81C_003079"
     CDS             complement(<1..>293)
                     /locus_tag="ACG81C_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5066141.1"
                     /translation="IITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIWLYRKLA
                     INRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFV"
BASE COUNT          136 a           49 c           30 g           78 t
ORIGIN      
        1 acaaacattt gaatgattaa aaatattaag acaccaaaca caaaatgatc aaatacttga
       61 tattgatgag caacataaga aacaaataaa ccaattgtaa gtaaataaat catgaatata
      121 gcagtaagtt tagaactgga ctgttcaaca atcctattta tagctaattt tctatataac
      181 caaataaaca tcagcaaaaa caaagaatca agcattatct ggaatactac ataaccatac
      241 ccttttatcc cttcaaatga aaacatatac gtcataacca tggaagtaat aat
//
LOCUS       DAZUMI010000311          291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000311 DAZUMI010000000
VERSION     DAZUMI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.337"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG81C_003080"
     CDS             <1..>291
                     /locus_tag="ACG81C_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066142.1"
                     /translation="AIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVK
                     KAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT           93 a           47 c           59 g           92 t
ORIGIN      
        1 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
       61 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      121 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      181 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      241 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt a
//
LOCUS       DAZUMI010000312          283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000312 DAZUMI010000000
VERSION     DAZUMI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.340"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..283
                     /locus_tag="ACG81C_003081"
                     /pseudo
     CDS             1..283
                     /locus_tag="ACG81C_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DAZUMI010000313          281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000313 DAZUMI010000000
VERSION     DAZUMI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.341"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>281
                     /locus_tag="ACG81C_003082"
     CDS             <1..>281
                     /locus_tag="ACG81C_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066143.1"
                     /translation="PIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQ
                     VITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTV"
BASE COUNT          109 a           42 c           58 g           72 t
ORIGIN      
        1 cctatcgctt cattggagaa taaggagcat ataataaaac acgaagcaaa gattggcagt
       61 caatgggtaa accttcctta taaaacaact ggtagttcta tcatatatag agtaactaat
      121 gagaagagcc aagtaatcac acttcctgta tatgcttatt taggaacgaa tgtagaaata
      181 aatcatcaac ctgtaaagga gcaggtgaat gctaatggat tggtacaaat aagcggagtc
      241 aaaggagttg ctgatattaa agtaagcaca agttatacag t
//
LOCUS       DAZUMI010000314          280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000314 DAZUMI010000000
VERSION     DAZUMI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.342"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..131
                     /locus_tag="ACG81C_003083"
     CDS             <1..131
                     /locus_tag="ACG81C_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066144.1"
                     /translation="RVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT           83 a           58 c           57 g           82 t
ORIGIN      
        1 aacgagtgat tcggattttt ccaaatatca attccatcac tcggataatt ggaacacttt
       61 tgatggaaaa agacactgag tggctggctt ctccacgaaa atatttagaa tttaattcga
      121 ataacattta gcaccatgaa cctgtgcgag tctcttcttt ctgaggagag acatcaagag
      181 tcgctgcgct actgctcctg acctctctcc tcagaaagaa aggcatggca gttaggttca
      241 caacgcaaaa cattgtgctt ttgatgaggt tgaactttta
//
LOCUS       DAZUMI010000315          275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000315 DAZUMI010000000
VERSION     DAZUMI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.343"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           89 a           47 c           44 g           95 t
ORIGIN      
        1 aagtgagaaa aaaggcttat aattggagtg gtagttaatt agtgacttat ttttgatttt
       61 atagcactga tactataaaa tatagatatc atcatattac acaatcttaa tactaactta
      121 aaaaatatct cctttcacaa gtatggtgat aaaattcgtt ccgggctacc tttttaggta
      181 gcctacttta atctttatac ctttctggat caacgaaagt atactttata tagtcataac
      241 gccgatgatc gctacgtgcg tccggcacgt cagtc
//
LOCUS       DAZUMI010000316          266 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000316 DAZUMI010000000
VERSION     DAZUMI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.345"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACG81C_003084"
     CDS             complement(34..>266)
                     /locus_tag="ACG81C_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066145.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAZUMI010000317          253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000317 DAZUMI010000000
VERSION     DAZUMI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.denovo.347"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG81C_003085"
     CDS             <1..>253
                     /locus_tag="ACG81C_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5066146.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZUMI010000318        14812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000318 DAZUMI010000000
VERSION     DAZUMI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.071"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..578)
                     /locus_tag="ACG81C_003086"
     CDS             complement(111..578)
                     /locus_tag="ACG81C_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5066147.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            701..1552
                     /locus_tag="ACG81C_003087"
     CDS             701..1552
                     /locus_tag="ACG81C_003087"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5066148.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(1803..3761)
                     /gene="nagE"
                     /locus_tag="ACG81C_003088"
     CDS             complement(1803..3761)
                     /gene="nagE"
                     /locus_tag="ACG81C_003088"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5066149.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3758..4630)
                     /locus_tag="ACG81C_003089"
     CDS             complement(3758..4630)
                     /locus_tag="ACG81C_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5066150.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(4972..5844)
                     /locus_tag="ACG81C_003090"
     CDS             complement(4972..5844)
                     /locus_tag="ACG81C_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5066151.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            5972..6541
                     /locus_tag="ACG81C_003091"
     CDS             5972..6541
                     /locus_tag="ACG81C_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5066152.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            6608..7345
                     /locus_tag="ACG81C_003092"
     CDS             6608..7345
                     /locus_tag="ACG81C_003092"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5066153.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            7576..8844
                     /locus_tag="ACG81C_003093"
     CDS             7576..8844
                     /locus_tag="ACG81C_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5066154.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            9331..9795
                     /locus_tag="ACG81C_003094"
     CDS             9331..9795
                     /locus_tag="ACG81C_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5066155.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            9779..10408
                     /locus_tag="ACG81C_003095"
     CDS             9779..10408
                     /locus_tag="ACG81C_003095"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5066156.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            10762..12240
                     /gene="msr(C)"
                     /locus_tag="ACG81C_003096"
     CDS             10762..12240
                     /gene="msr(C)"
                     /locus_tag="ACG81C_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5066157.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            12287..12733
                     /locus_tag="ACG81C_003097"
     CDS             12287..12733
                     /locus_tag="ACG81C_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5066158.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(12932..13447)
                     /locus_tag="ACG81C_003098"
     CDS             complement(12932..13447)
                     /locus_tag="ACG81C_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5066159.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            13564..14766
                     /locus_tag="ACG81C_003099"
     CDS             13564..14766
                     /locus_tag="ACG81C_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5066160.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4626 a         2680 c         3054 g         4452 t
ORIGIN      
        1 cctgagcatt agagaggaaa aaggggcatg acaaggcttt tgtcccccgg agaataaaag
       61 aatttatgta gacgattccg atcagaaaaa tgcaggcaca gtagagagaa ttataaaaat
      121 ctctctacga ggcctgtgtt ttctggtctt ttttcttttt ccaaaacggc tattttatgt
      181 atcagactat caagtgtgat cttagccatt tcttcttctg cctttttctg cagccgagcg
      241 tagctttctt ctaaagcttc ttggatattc gcaccaacga cgcaatttgg atttgttttt
      301 ggatcgactt ggataagctg tgtattacct tcgatgctgc gatagacatc tagtaaagaa
      361 atctgatctg gacttttggc aagggaaggc tgcggttttc cggtttttgt aaagatcaaa
      421 ccggattttt tgagctgact catgatttta cgtacatttg ctgcatttgt ttcgatacta
      481 ctggcaatca tctcactaga aagtgatcgg gtatcttgaa acatttcaat ataggcaagt
      541 acatggattg catcactaaa ttgcagagaa tacttcatca gttgtcatcc tttcattttt
      601 tttgcttgac ttatcttacc acactcttta tgataaaggt gtaatttata aatgtacagc
      661 tgtaaatctt aacaaataaa aagaataggg agtggcaaat atgcattatt tagtaacagg
      721 agcaactgga ggttttggtg gttatgcgct ggaattctta aaaaagatgg ttccacaaaa
      781 tgaaatctat gtactagtac gtagtgaaga aaaaggagaa gcactgaaag aagctggatt
      841 taatatacgt attggtgatt atgatgattt agactcaatg aaagaagcgt tgaaaggaat
      901 agatcgattg ctttttgtct ctggcgttcc aggaaatcgt caagccgaac ataagaatgt
      961 agtgaaggca gcacaagcag ctggggtatc ttatatcgct tatacaagtt ttgcagatgc
     1021 cgatcactcg atcagtgtat tagcgccaga tcaccaattc actgaaaaaa tgattaagga
     1081 ttcaggtatt tcccatacat ttttacggaa taattggtat gtagagaatg aattacctat
     1141 cattgggcag gctttaaaaa ctggtcaatt cgtttatgct gcaggaaatg gaaaaacagg
     1201 ttgggcattg aaacgtgaat atgcagaagc tgcagcaaaa gtactagtag aaagtgactc
     1261 tccagaaatc ttagaattat ccggcaatct aactgactat gaagaattag ccaaggcatt
     1321 agagcgtgcg acaggtaaag agttagaaat tattgaagca agtgatgcag cgtttgtcga
     1381 aaatctaaaa gaagccggat tccctcaaga agcagctgac atgtttttag cgatccaaca
     1441 tgatattaaa aaggatcagt tagctatttc ctctgatgat ttagaaaaag tattgggaaa
     1501 accattaact tctcttgaag cagcattaaa agaaatattg gatcaggaat aatgtataaa
     1561 gaggttgtga aaaagccgat tagttttgag tatgaagaaa aattttgtga aaaagctgtt
     1621 ctgcatcaag taataagaag agtcaaataa aaaatagctc ttcatatgtt tcgtttagct
     1681 ggacttatta ccgcggagtt ggcaggagaa cgccgttttt attcggaaaa aggagctgag
     1741 acaagatttt tgtctcagct cctgtttgtt tatcgataaa aaagtctggg gaagacgatg
     1801 gctcaggctt tgttggaaac agagccgatt ttatcatttg cttttacatc acgtgcgggt
     1861 tcgatttcta agctttggac tttgtcgcca tttgtgaata caacgaccat atccgtgttt
     1921 ttgcctgcag attgaatagc ggcaagatca actaaagcaa tcaattgacc tcttgcgact
     1981 ttttgacctt cttctacata taaagtgaat ggttcgcctt ttaagtcaac agtgttgatg
     2041 cccatatgaa gcaagacttc gataccagca tctgtttgga ttccgacagc atgtttcgtt
     2101 gggaaaatgt ttgtgatcgt accagcgatt ggagagaaga tttctccatt ttctggtaat
     2161 acagcatagc cgtcacccat cattttttcc gcgaatacat catctgaaac ttcagtgatt
     2221 ggaatcagtt tcccatttgc gatagcgtaa atatcttgtt cgatcgtttc accggcaaat
     2281 gtttctgttt taaccacggt gtttgtttgt gcaggtcgtg ctgcagcacc accatgtagt
     2341 tttgccatct catctgcaac gaattgtact tctgtaccta cgatgacttg cgcatttttt
     2401 ccgtcgatga tcttcatacc aggtacgcca gtggctttga ttttattttg atcgatcagg
     2461 cctgtatctt tgacttgcaa acgaaggcgt gtagtacagt tatcgatcga agttacattg
     2521 tcggctccgc ctaatgcagc gtagattttg cttgcttgtg ccgcatattt attgtctgaa
     2581 ttagtcgttg cagtatcgtc atctgaaaga tctgcttcct cgcggccagg agtcatcaga
     2641 tggaattttt tgatagcaaa gttaaagcca aagtagtaaa tcactgctgt tactaatcct
     2701 tggacgatca acatcaatgg ctgattcgca atcggatttt tcaaagatag gaagtaatcg
     2761 acaaatccgg cactaaaggc aaatcctgct gtccaatgga agaatgcact cactgctaaa
     2821 gaaatcccag tgaaaattgc atgcaagaca tataatggcc aagcaacgaa cataaatgaa
     2881 aattcaagtg gttctgtcac accagtaaag aatgaagcaa aagctgctgc tagcatcaag
     2941 gaagctgttt ttgctttgtt ttctggacgt gcatttctgt aaatagcata tgctcctgct
     3001 ggaagcccga acatcatgat tgggaaaaat ccagcttgat acattcctgt tacgcctttt
     3061 gttccttcag aagccaaaaa tttaccaata tcattgattc ctgctacgtc aaaccagaac
     3121 acggagttca ttgcttgatg caatcctgta gggatcagta aacggttgaa gaagccataa
     3181 agacctgcac caccccagcc taaactcaaa atgaatttag caaaagaaac taaagcagag
     3241 taaaccggag gccatacgag cagtagcact gcactgatca gcaacatgac aaaggcagcc
     3301 aagattggca ctagacgctt gccgctaaag aaagataaag ccatcggtaa ttttgcttcg
     3361 tggaaacgat tataaagagc tgccgcaatc aatccggcaa taataccaat aaatacgtta
     3421 ctgatctgag caaaagatgg atcaactgat tctggtttga tattcaataa agtagctact
     3481 gaatcagttt ttaaaacatt tactggaact tcaaatgcga ccaaacctgc caaggcagca
     3541 gcacctgatt tgtccactga catccctagt gctaacccaa cggcaaacag caaaggtaat
     3601 tgacctaaaa ctgcgttacc gccagcttgt aaaaaatgag cgacaatgtc gttactataa
     3661 gatgcccacc agtttccgat ccctactagt aaagcagcag caggtaagac cgcaactgga
     3721 agttgaaggg aacgtcccat tcgttgtaga tatgctttca atttttttcc tcctcatgat
     3781 tgttaacatt tgtttgtacg attgcgcttc ttaaacgctc gatatggatg gccaagtaac
     3841 ccagttcttc tttcgtgatc gcgagctggt attctttggt cagcaacaca cggatttttt
     3901 tagaaatctt atagctttct gggtattttc gtttgaccat ttctacgatt tccgtgtcta
     3961 aaacagcata ttgttgcgca tgggttcgtt gtagaagcag tcgtaaatga ttcaccagtc
     4021 ttgaataatt cagtgaaaat ggttctgaat ggatatctgt ttccaattct gattccacta
     4081 aatggatgat gtcagataca atcgtcactt cacggatact gcgatggtta ttgttccgac
     4141 cgcttcgtcc gctgtgaata tggattgcga tatatcctgc ttcgtcataa gtatatggaa
     4201 tcccaagctc ttttgtcaga tactctactg cccattgagc aatactgaat tcatcactgt
     4261 aaagcacttc gatttctctt aaaagtttgt tgcggatgac gataccgtta ttaatgttct
     4321 ccgcagaaaa agcaatgtgg tcggtcaaag cgatcaatac atgttcattc aacttttcca
     4381 tcaaaacagt ttccgcatga tcaatgatcg tttctgctgc aaataaaaag cgttcatcaa
     4441 tctgattaag taaattttgc aacttgattt gaccttctgg ttccatgaca aatatccgtt
     4501 cgacatcacg atcaaaaatc aaatcatttc tttttctgtc aaatccaatc ccttttccaa
     4561 tagctacttt ttcctgtcca ccgtcatcta ccaatactgc attttgatta aagatctttt
     4621 tgattttcat gcgcgcaccc tttttgaaga tttggttttc acgtttgcta gaccagttga
     4681 ttgcggccaa acaaaaatag acacgaaaaa cctcctaggg aaaccatgtt cctcagagat
     4741 ttttcatgcc tagtatgatc tagtcacatg aaacgaatta ttgttaacgt aatcaatttt
     4801 atatagacag ttatgttttg tcaattcttt ttttataatt atagtaattt actggaatta
     4861 tcagaaataa aaggaagaaa taaattaaga aacttatgtt atttaatcgt tggatctttt
     4921 gcttatctta ttcgttgtta aaaagctgaa agcgaaggaa aaaaactcta cttaactgta
     4981 tttctccgtt aggacatcta aaaatttacg agcagcagga gaaagaggct ggtctttttt
     5041 ccaaatgaga ttcagtgaag cttgtaatgg cggatcgagt gggatgaata ttttttccgt
     5101 cccataagta ttgatgatcc catctagagc taatgcacca aaagtcccgt tatcgaccat
     5161 caaagaagca ttgtacaata agttatatgt tccgacgatc gataactgat ctatgctgaa
     5221 acctagccat tcagctagtt gactgtccac tgacgtttgc cgggaaatga tcaaaggaac
     5281 agttttcagt tcctgcggat ggatgacggt ttgctttgct aaaggatgat ttcgatgtat
     5341 caaaagtccc caacgatctt gggctggcag tcgaaatgac tggtattttt gtttgtttgt
     5401 cggttcgatt acgagtccaa aatcaagtaa tccgctatcc agtttgtcta tgatatcatc
     5461 tgcattcccg ctatataaat gaaactggat atcaggatgc gccttgatca acgaatgaag
     5521 gctatcagaa atgaaagaga ttgctttagt ttcgcctccg ccaatgtaga tttctccaga
     5581 gatgattgcg tcatttgtta aattgttcgc cgttttttct accaacgaca agatttcttt
     5641 tccttttgaa taaagatatc gaccagaatc cgtcaactgt atctctcggg agccccgagt
     5701 gaaaagtgtc acaccaagtt cttcttctaa atctttcagt tgttttgaga gagtaggttg
     5761 cgtgatatgc agagattttg ctgctcggct gatatttttt tctctagcta ctgctaaaaa
     5821 atacgtcagt atacgaatat ccattttttc cctcctgttt ttaaaaaagc ataccactaa
     5881 gtatagttaa aaggaatagt taagtatgtt ttataagtat tagacatgat aagccgtccg
     5941 ttataaagta aggataaaag gagggagcaa aatgacaagc ctcatcgaaa aacaaatcgt
     6001 caataaagag attcgagtaa cggatccgct ctttgaagtg atccatcaga tccaagctga
     6061 aaacgaagag cctttagcag cgctgaatac tgggtaccat gaaccagcag atatccgcaa
     6121 acggctagaa aatatcatta gtgacaaagt agatgatacg gtgactgttc tgctgccttt
     6181 ttatacagat ttcggcaaac atattagtat agggaaaaat gtctttatca atcgtcaagt
     6241 gatgttcgtt gacttaggtg gcatctgttt agaagattca gtattgattg gtcctcgtgt
     6301 caacctcatc acggtgaacc atctaaccga tccaaaagaa cgaagaggct tgttagtaaa
     6361 gccaatccac atcaagaaaa atgcttggat cggagcagga gcaactattc tgccaggcgt
     6421 aacgattggc gagaatgcca ttgttgcagc gaatgcaact gttacgaaag atgtcccaga
     6481 caataccatc gttgcaggaa tccctgccaa aatcgtcaaa cctgtcgaac ggattatttg
     6541 aacagtaaga tgaaataaga gatagtgagt ataagcaaga gatttttcaa aacaggagga
     6601 gaatagaatg aatgatttat cagaaaaagt gattgtcatc atgggcgctt caagcggaat
     6661 cggtgaagca acaacgaagc tattagcaga aaaaggggct aaactagtta tagctgcacg
     6721 ccgagaagat cgcctaaaag caataaaaga atctttacca gaagctgagc tatatattca
     6781 aacagcagat gtaagagatt ttgcacaagt ccaagcagtt atcgatttag cgatggaaaa
     6841 gttcggtcga atcgatgtat tgtacaataa tgcaggaatc atgccaaccg ctccattagt
     6901 tgaaggtcac cgtgatgaat ggcaaaatat gcttgatatc aacatcatgg gtgtgttaaa
     6961 tggaatttcc gccgtacttc caatcatgga aaaacaaaaa tctgggcata tcatttcaac
     7021 tgattctgtg gcgggacatg ttgtttatcc tgattccgcc gtttattgcg gaacaaaatt
     7081 tgctgtccgt gcaatcatgg aaggattacg tcaagagcaa cggcaaaata acatcaaatc
     7141 tacgatcatc tctccaggag ctgttcaaac tgaattatat caaaccatca gcgacaaaaa
     7201 ggctgctctt gaacttcatg aagcgcaaaa agaatgggga ttgacctccg aagatatagc
     7261 ttctgctgta gcatttgcga tagaaacgcc agaccggatg tctgtcagta acatgattat
     7321 ccgtccgacg acacaggaag tataatccat aacggataat atccaaagag aattacttgt
     7381 taaatagtta ttcaaacgac catcaattta aggcgtgaat ggagaagaat attcttcatt
     7441 tacgtctttt tatgtagaaa aagcaaattt atcaaaaaga aacaaatgtt atttaaatat
     7501 ggacattgac ttgcttttac ttttttagtt acactgttat ttaggaaaag tttcataatt
     7561 caaaggagaa aaaagatgaa agcaaagaat ttattattta gcgttattct tttaggaaat
     7621 ctagcagttg ccaacacaac agtgctagct gaagaatcga cagaaactgt cgcaactcag
     7681 acatcgagtt cttcagaaac aggaattgaa aacgatacag agagtacttt gcagtcagct
     7741 actgtctcag aaaatacaat ttcaaccgaa gaaccaacaa ctgaaacaac agaacaaaca
     7801 caaaccgaag acagtacatc agcagaagct cagaacaatc cagagaatgc agaagaaatc
     7861 acgaaccaat tgacagatgg gcaaacattg acacataccc atcaagcaga tttgtctgaa
     7921 ttcgaaaact atactggcta tgctttttat gctcgctcat ctgctaattc tcaacaagcg
     7981 ttcattgata gtattgcctc aactgcacaa agtttggcaa gtgccaatga cttatatgca
     8041 tcagtcatga ttgctcaagc catcgttgaa agtggctggg ggaacagtac tttggcttct
     8101 gcccctaatt acaatctatt tgggattaaa ggaagctata atggccaatc tgttacgatg
     8161 cctacttcgg aatatgtgaa tgggcaatgg attacggtga atgccgcttt tagaaaatat
     8221 ccttcgtaca aagaatcatt gcaagataac gtaacggtat tgaagacgac ttcttttcag
     8281 ccaggcgttt actactattc cggtgcatgg aaaagcaata cgaactctta caaagatgct
     8341 acggcttggc taacaggtcg ttatgccact gcgcctaatt atggttctac attgaacaat
     8401 gtaatcgaaa cctataatct gacacagtat gatacagcac caacttcaac ttctccgatg
     8461 tatcgtctgt ataaccgtca tacaggagaa catttatata cattgaatgc cggagaaaaa
     8521 aattatctgc caactgtagg ctgggaatat gaagggattg cttggcaagc accaaacagc
     8581 ggacaacctg tctaccgttt atacaatcct tacagtggag accatcatta cacaatggca
     8641 caatctgaga tcaactcttt aacaaaaatc ggttggcgtt atgaaggtct aagtttttat
     8701 tctggtggtt ccaagccaat ttatcgacta tttaatccaa atgaaaaaac tggtacccat
     8761 cactatacct tgagtgctaa ggaaagagat tggctaacac cgatgggctg gagatatgaa
     8821 ggaataggat tttatggtta ttaaaaagag gtagtgaaaa agctgccctg catcaagtag
     8881 taagaacagc caaacaaaaa cagattttca tgttttttgt ttggttgaac ttattaccgc
     8941 agatgcaagt ttttgaacac cgtttactca aaaaaggttg tgacaacatc ttgtcataac
     9001 ttttttatta ctgtttaagc atactgatcc aatttctaat attcgacggg attcttgcaa
     9061 aaggcgattt cggaaatttt tggcggtgta aaattttcta ctgcagaaga gctatcttct
     9121 atagtttgat aagaatcaat aatcttggtt ttagcgttgt gctttcttta tcattttttc
     9181 cgttcattct acaaaaaatt tcttttctct atacttttct attgagagcg ttttttttat
     9241 tacacagctg attctttgac ttttttcttt ttttattcta ttattgacaa gtcaatcgtt
     9301 gacgagtcaa ttattagaaa ggaggaaatt atgtctttag aagagattag tcacctttta
     9361 tatcaaatca aattagccga tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata
     9421 agtcttacta gatacgagat catgatgaga ctgaaagaag aaaatccatt gctacaaagc
     9481 agtttgcaag aaaagttgca gattgatcaa ggagcagtta ctcgtcatct gaaggtattg
     9541 gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga acaaccggga agtgtatgta
     9601 gcgctaacag aaaaagccca gaagaaaatc gagcattgtg aaatggacca taaagataca
     9661 ggttccgtgc tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta
     9721 gataaattga atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat
     9781 ggaacaaaaa aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga
     9841 tgaaacatac aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc
     9901 accttcttct gtcaatatgc aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa
     9961 agaaatcttg cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat
    10021 ggtcatgatt tttggcgata tgcaatgtta cgaatatggc gaagaaatct ataaccaagc
    10081 ttacgaatca gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc
    10141 ttattacaag agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt
    10201 agcggctatg caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg
    10261 tggagtcgaa gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc
    10321 agttattatt ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc
    10381 accagaaaag atcactacgt tcgaataaaa ggttttcagt tactattcag tagctattgc
    10441 tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg
    10501 atgaaattac gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa
    10561 caacaatttc atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc
    10621 tatgacagat ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg
    10681 cagttttttt gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca
    10741 tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc
    10801 agctttggca accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt
    10861 atagggatca taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt
    10921 gaactttttc ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa
    10981 ttaacctatc tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta
    11041 agactggaag aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg
    11101 atacgtcttc tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta
    11161 gatagaaaaa gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt
    11221 tttgttagtc atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa
    11281 gacggagaaa tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg
    11341 gagaaaaaga ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa
    11401 aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag
    11461 aaaaagcaac aaatcagacc ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa
    11521 aaagccatcc aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca
    11581 accaagccgc agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac
    11641 agccgctatc ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg
    11701 gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt
    11761 tcaggtaaga caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct
    11821 ccgaaagtaa gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc
    11881 atcattcgtt ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg
    11941 aatcacttag ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga
    12001 gaagcgaccc gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta
    12061 gatgaaccga ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa
    12121 atatatccgg gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct
    12181 gatgaagttt atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa
    12241 atgtgctacg atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa
    12301 aaaagaagga ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc
    12361 gtataattta tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc
    12421 tggattcgtg gtggctttca ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat
    12481 tgcctttaaa ctaccagtaa atccagaaag caaggtccaa atgatcttaa ctatttcatg
    12541 cttgatggtt ttaggaatgg tgacatgtat gtctttattc ggtgtgatca tggaacaagg
    12601 aatacctgat caattattct ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc
    12661 tttaccgttg cagttgatca tcgttggccc gctttcaaga agtgtgctaa aagcaattca
    12721 aagtaatgaa tgatggaaca ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata
    12781 gtctcttgaa tgcagttcta gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt
    12841 cggatcgctt tttcttttcc aactgtattg ctcttttatt gaagataatt tgctcttcaa
    12901 tagatatttc tagaaaggtt ctgtttatcc cttactcaaa aaactgctgc atttgttctc
    12961 ttacttgctg acaccattgg agttcctgct gacaaagcat aattctgcgc tggatcacaa
    13021 cataacgtcc aaattcttgt ttcttgtcta gcgagaggtc attcaatttt ttttgatttt
    13081 tttgcatgat tttgttcagc tgttgttctc tagtgaatag cagtccagta gctgttgttt
    13141 tatcgagcgt cgaaaaagca tagatttttg ctagaaattc atctttttgc gtaggagcgg
    13201 gagtttcctc tttgatccat tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga
    13261 tctttttttg agagtgctcg tctttgtcca aaacggtcac ataaccctgc tggtctaact
    13321 tggcaagcgt tgtatagatc tgactgtgat gggcttccca aaaaattgtg atcagctgct
    13381 tcaattcata cccagaacgt ggtttggata aaagcacgca taataatata taggctaaag
    13441 tattcataaa aatcctacct ctcgtcttat tttctttatt atatcacagt aaagtttcca
    13501 cgttttacat gtgcatattt acatgtaaaa taaatcatgt atgatgctaa tagagagaaa
    13561 gggatggaaa tattggagaa tcaaatagat atacaagaac aaacaaatga agaaacaaag
    13621 gggaaaagtt cagcgctagt tgtagcagct acctgtatga tcgcttttat gggagtgggt
    13681 ctagtagacc caatcttaaa aacgattgct gcacagttaa acgctacgcc agctgaaacg
    13741 accctgttat ttactagtta catgttagtc actggtgtgg tgatgctatt tacaggtttc
    13801 ttatctagtc ggatcggttt gaaaaaaaca ttgatgacag gattgtttat catcgttgta
    13861 ttcgctggtc ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc
    13921 tggggtgtcg ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta
    13981 gttggtaaca cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct
    14041 gttggccctc tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg
    14101 gtagcatttt tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag
    14161 aaaccaaaga gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt
    14221 cgttcagttg gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat
    14281 gcgccatttt tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg
    14341 ggtgtgcttc tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg
    14401 acttatcgga caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga
    14461 gctggaactt ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa
    14521 ggaattatca atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac
    14581 gttgcttcgt ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt
    14641 gcaggtgtga ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt
    14701 gttcttatca gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa
    14761 gaataagaaa tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DAZUMI010000319        10451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.103, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000319 DAZUMI010000000
VERSION     DAZUMI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.103"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..984
                     /locus_tag="ACG81C_003100"
     CDS             88..984
                     /locus_tag="ACG81C_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5066161.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1134..2075)
                     /locus_tag="ACG81C_003101"
     CDS             complement(1134..2075)
                     /locus_tag="ACG81C_003101"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5066162.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2201..3322
                     /locus_tag="ACG81C_003102"
     CDS             2201..3322
                     /locus_tag="ACG81C_003102"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5066163.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3580..5082
                     /gene="eat(A)"
                     /locus_tag="ACG81C_003103"
     CDS             3580..5082
                     /gene="eat(A)"
                     /locus_tag="ACG81C_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5066164.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5149..5676
                     /locus_tag="ACG81C_003104"
     CDS             5149..5676
                     /locus_tag="ACG81C_003104"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5066165.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5887..6948
                     /locus_tag="ACG81C_003105"
     CDS             5887..6948
                     /locus_tag="ACG81C_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5066166.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6951..7847
                     /gene="murQ"
                     /locus_tag="ACG81C_003106"
     CDS             6951..7847
                     /gene="murQ"
                     /locus_tag="ACG81C_003106"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5066167.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7861..9843
                     /locus_tag="ACG81C_003107"
     CDS             7861..9843
                     /locus_tag="ACG81C_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5066168.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9908..10354)
                     /gene="nrdI"
                     /locus_tag="ACG81C_003108"
     CDS             complement(9908..10354)
                     /gene="nrdI"
                     /locus_tag="ACG81C_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5066169.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3405 a         1848 c         2269 g         2929 t
ORIGIN      
        1 tacaaatata ggaaacgttt tactggcttt ttactattta aacaggctat cataggaaaa
       61 agtgacagag aaagtgaggc ggaaaagatg aacaaaaatg acgtactgct agttgcaaaa
      121 aataaatcac tttatttttt gccttccatc cgctctttag atgtttggtc cggaaaagat
      181 ttactacagt cggaattcat taaatcagct agacaacagt tggcaaatta tcagctgatc
      241 gtttttttag attatggatt tgatatggag atggctcgac gcgtgcgccc atttaccaat
      301 gcaaaaattg tgttattttt ttggaatcac ttcaaagcag aacacgaggc gatgctgaag
      361 caggcacaag atgaaccggc aatcgatgag atttatcatt ttgatttttt ggaagcaaaa
      421 gaacttgggc tgaaacataa tagttcattt tattcgaaac atatgaattt ttcttctagc
      481 gaaccaaaga cggatctatt ttttggtgca acgaataatg ggagaaaaga acgagcagaa
      541 agctataagc aggaatttat aaaaagagat gttacgacca actatttcat tcttccacat
      601 cgagggaatg aacaacctgg atatcttact tatgaagagt acctgaaaaa aacaggggaa
      661 tcgcgcggga ttctggaact gttgcgggag ggtcagcaag gagtgacttt gcggacattt
      721 gaatccatgt attttcaaaa gaaactagtg acggataatc aagcgattgc tcattatcat
      781 ttttatcgtc ctgaaaatat ttttttgctt cgagaacgtg atttagatga attaccggaa
      841 tttctgcata caccatttaa gccaatccta tcagagctat tggaatttta tgatgcagaa
      901 aattgggcaa aacgatttat cactaacgat tccactattt atgagcaata cgaatatgac
      961 ctctcctctg ttgaaaacaa ataaaaaacc tacattttga agagattatg tactgaaaca
     1021 aacaagcaca attcttatgg catcatgaga gttgtgtttt ctttttgatc ttttattggg
     1081 cttaaaaaaa gagaaccagc tgctgattca gctgattctc atcagagagc cttttattct
     1141 gcgccaagga ttccttcttt tgcatggact aattgggtca aaaaggcaca atatggtgta
     1201 gcaatatcat atttttgtcc ttttcgccaa atggcaccat tgatgtagtc gatttcagtc
     1261 aaacgatggt ttttgatcag gtcttggtac atagatgggt aatgcaaccc gatgttgtca
     1321 ggatcataag tagaagcaat atgttggtac acttcttctt gatctagtat gattccttct
     1381 tttgctgcga tagaagcaaa ttcagatacg attgttcgaa ccatgctctc tgccgctttt
     1441 tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc ataaaccatt caatgttcca
     1501 tttacacaag cttttcgcca aatggaataa cgaacgttat ggctatagat cggatttaaa
     1561 tttgcttctt gaaagacttc tacaactttt tttgtaaatg cttcgccttc tggttccaag
     1621 ttctctagtt cgatttctcc atcacctaac aatgtgactt ttccgggacc agctagacca
     1681 gctgtccaca tcgtgatccc aaataagata tttttctttg gcacatattt ttccagtaca
     1741 tcttcatgac ctaatccatt tagtaaacac aaaacataag tattgtcagt aataatggat
     1801 tggatcgaac aaaacatatc atctaattga ttggcttttg ttaatgctac gatcaagtcc
     1861 acgtgttcgt tgctttcgat gatttcttct ggtgaataga ttggcagttt tgcgaccact
     1921 tctttaccgt taaagtcagc aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc
     1981 caacgatcga tcaagaggac ctcatttccg ctttgatgta gcatcaatcc gattcggctg
     2041 cccattgctc cggccccagc tattgcgatt ttcatgtttt ttctctcctt tattcaatga
     2101 gttccctttt agtatataga aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg
     2161 aaaaaaagtg tcttacttaa gaagaaagtg agggatacag atggatattt tggcggcaat
     2221 tgattcattt aaaggaagtg cttcttcatt tgaactaaat caggcagttc taaatggctg
     2281 ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca gatggcggag aaaatactgc
     2341 agaagcaatc tattacgcgc tcaggggaac atggcgtacc attgatggga tggatctctt
     2401 attccgtcag cgaaaaatca gctatttatt gacaacttgg caaggaaaaa acaccgcagt
     2461 catcgaatct gcacaagcat tggggttaga tctacttgat aaaccaacgg atcaaactat
     2521 ccgacttgct tccagttatg ccttaggaga acttattaaa gatgcactgc ttcaagaagt
     2581 agaacaaatt attgtcactt taggaggaag cgggtgtagc gatggtggtc taggattact
     2641 ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat tatctactaa
     2701 aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc
     2761 agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg gaagacaaaa
     2821 aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga ttgctcagca
     2881 aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag cagcaggcgg
     2941 tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg atttagtcag
     3001 ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta caggagaagg
     3061 gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag tgcttgcaaa
     3121 aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc tagaaggact
     3181 ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt tagaagaagc
     3241 aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac ttagcagatt
     3301 tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc gtaatttcaa
     3361 atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca
     3421 ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga ttggcttggt
     3481 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
     3541 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
     3601 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
     3661 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
     3721 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
     3781 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
     3841 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
     3901 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
     3961 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
     4021 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
     4081 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
     4141 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
     4201 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
     4261 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
     4321 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
     4381 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
     4441 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     4501 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     4561 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     4621 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     4681 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     4741 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     4801 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     4861 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     4921 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     4981 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     5041 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     5101 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     5161 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     5221 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     5281 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     5341 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     5401 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     5461 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     5521 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     5581 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     5641 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     5701 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     5761 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     5821 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     5881 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     5941 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     6001 gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag
     6061 aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc gggtttttca
     6121 ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat ggattatcat
     6181 atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt gaatgctagc
     6241 acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt ctcacagatt
     6301 gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga atggtatcag
     6361 aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt accaggagat
     6421 acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca tcgagggatc
     6481 catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt ttacatcgga
     6541 gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa agaacagaca
     6601 gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt agggaagcat
     6661 acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg ttttcgagag
     6721 attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat tactttagat
     6781 aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga tttgccagcg
     6841 gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt gatcaaccaa
     6901 atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa atgagcaaaa
     6961 tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga ttagatgaga
     7021 tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg gccgctgcgg
     7081 ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag tcctttaaaa
     7141 aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt gtgttagatg
     7201 ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt ctaattgctg
     7261 gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat gagctagggc
     7321 aagaagatct aatcaagctg aatttgacaa aaaatgatat agttattggt atcgcagcta
     7381 gcggtcgtac accatatgtg atcggtggat tagattacgc aaaatcgatc ggtgctgtca
     7441 caggaacgat ttcttgcaat aaacaagccg aaatcagtaa acacgcggat ttcccaatcg
     7501 aagtcgatgc aggtccagaa tttttgacag gctctactcg attgaagtca ggaacagcac
     7561 aaaaacttat cttgaatatg atttctacta tcagtatgat tggtataggt aaagtattca
     7621 ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact aatcgaacgc tcaaaacgaa
     7681 tcatcatgca agcgacagaa gctgattacg aaacagctga aaagtacttt gaagaagcag
     7741 atcaaaatgt taagctggct atcgtcatga ttttgacaaa tagtgataaa gaggaagctc
     7801 aagcaaaatt agctgaagca aacggatttg taaaagggac cttatgaggg ggaaaagacc
     7861 atggcaaaaa acgaactgac tttggaagaa ctggcaaaac aaatttataa aggggcaggc
     7921 ggcatgggca atgtagaaag cattgtccac tgcatgactc gtgtgcgtat gaagatcatc
     7981 aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc caggagtgct tggtgtagta
     8041 gaagatgaac agcttcaagt catcatcgga cctgggaaag tgaacaaagt agcacagcaa
     8101 atggttgatt tggcaggcgt gaaattagga gaaaaacttt cttctgtaag tacagctaca
     8161 gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac gggcacaaga aatgaaagcg
     8221 gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg tattaaaaga catttctaat
     8281 atctttgtgc caatgattcc tgcatttgtc ggtactggga tcgtggcagg gattgccgca
     8341 gttttagcaa acttagtcac agccggaaca cttgatgcag caacatggca acaatatatc
     8401 gatgtgatga acatcttgaa aaacgggatg tttagctacc tagttatcta tacaggtatc
     8461 aatgctgcac aagtttttgg tgcaacacca actcttggtg gagttatcgg ggccgttgtg
     8521 atgctgacag ggatgaatcc ggaagcacca ctgaaaaact tatttactgg tgaagctctt
     8581 gcagctggac aaggtgggat cctaggtgtc atcttcgctg tttggctatt gtcaatcgtt
     8641 gagaaaaaac tgcataaagt agtacctgat gcggtagata tcatcgttac accaactctt
     8701 tcattgattg ccatcggtct gattgaaatt ttcttcatca tgcctttagc aggtttcatt
     8761 tctgatggca tggtcggtgg gatcaactgg gtacttggcg ttggtggagc atttgccggc
     8821 ttcgttctag gcacattatt cttgccgatg gttatgttcg gactgcatca aattttgact
     8881 ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc tattgcttcc aattcttgcg
     8941 atggctggtg caggacaagt gggtgcagca ttggcattat ggattcgctg caaaaaagat
     9001 aaagaattaa cagaaatgat caaaggagct ttaccagtcg ggatcctagg aatcggcgaa
     9061 ccattgattt acggagtaac attacctcta ggacgcccat ttattactgc ttgtatcggt
     9121 ggaggtatcg gcggggcagt gatcggaatg atcggaaatg tcggggctat cgctattgga
     9181 ccttcaggag ccgctttgat cccattgatc tctgacggta aatggttagg ctacatcctt
     9241 ggattattgg cagcttatgc tggtggcttc gttgctacat tcttcttcgg tattcctaaa
     9301 gaccgtttag aaaaagaaga attagcgact gaaacagcca cagtaaatga agtgcctgaa
     9361 aaagtagtac ctcaaacttc agctgaaagt gtgactatcc tctcatcagt tgctgacgga
     9421 acagttgttt ctttagataa agcaagtgat ccagtttttg cacaaaagat gatgggtgaa
     9481 ggctactttg tagaaccaga aaacggacat atctattctc cagttactgg ggtagtcagc
     9541 tctgtcttcc caacgaaaca cgctattggg atcacaacgc caaatggttt agaaatcttg
     9601 cttcatatgg gaatcaacac agttgactta ggcggtacgc catttgatgt gaaagtaaca
     9661 gaagggcagc aagtgacacc tgatacgctt gtagcagaag ctgacttaga agcgatcaaa
     9721 gcagcaggca aagaaacatc catgatcgtc ttggtaacca atatggatcg tgtaaaaaac
     9781 tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac ctgtgatgga tgtcaaagct
     9841 taatatgata gaaacaatag aaacgaaaga agaaactgaa atctattttt cagcttcttc
     9901 ttttttatta tttattgttt tctgctaaaa tctgataaat tcgtgtcaca tcagatggtg
     9961 ttccccgtaa ttcataatct gccaagaaag gaaagccaaa tgtttgtgca tattgtttgg
    10021 ctgttagaca atactgatta ttgaagtttt tatttccgct accgactaca ccaagacaaa
    10081 gtttatgatt gtcttcatgt tccagatatt cacgcatcgt ttcggtcaag atttctgtat
    10141 caccgttatc taaaccattt ccgccgtcta aataagtagg aacgaaagta aaaaatggtt
    10201 ctgtttcttt cgtaaaatca ctattttcat ggatttcttt taaagtaact tcaggattga
    10261 caggatcttc tgcatgcatt ttttcagcat attctgctag atgtttggca aaggcacgcg
    10321 tatttcctga aatcgatata tataaaatgt tcatggatca tcctccaatt ctttcctttt
    10381 agtatagcat cttttttaag ttttttggtg gtgtaatttg gaaagggact aggcgctttg
    10441 cttgtataat g
//
LOCUS       DAZUMI010000320        10087 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000320 DAZUMI010000000
VERSION     DAZUMI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.108"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACG81C_003109"
     CDS             353..523
                     /locus_tag="ACG81C_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066170.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACG81C_003110"
     CDS             537..1139
                     /locus_tag="ACG81C_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5066171.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACG81C_003111"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACG81C_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACG81C_003112"
     CDS             2143..2640
                     /locus_tag="ACG81C_003112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS5066172.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACG81C_003113"
     CDS             2641..3057
                     /locus_tag="ACG81C_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS5066173.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACG81C_003114"
     CDS             3077..4621
                     /locus_tag="ACG81C_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5066174.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACG81C_003115"
     CDS             4764..4988
                     /locus_tag="ACG81C_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5066175.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACG81C_003116"
     CDS             5003..5872
                     /locus_tag="ACG81C_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5066176.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACG81C_003117"
     CDS             5853..6587
                     /locus_tag="ACG81C_003117"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5066177.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACG81C_003118"
     CDS             6620..7528
                     /locus_tag="ACG81C_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5066178.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACG81C_003119"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACG81C_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS5066179.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACG81C_003120"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACG81C_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5066180.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACG81C_003121"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACG81C_003121"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACG81C_003122"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACG81C_003122"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAZUMI010000321         8650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.122, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000321 DAZUMI010000000
VERSION     DAZUMI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.122"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..573)
                     /locus_tag="ACG81C_003123"
     CDS             complement(202..573)
                     /locus_tag="ACG81C_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5066181.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            718..903
                     /locus_tag="ACG81C_003124"
     CDS             718..903
                     /locus_tag="ACG81C_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5066182.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1061..1228
                     /locus_tag="ACG81C_003125"
     CDS             1061..1228
                     /locus_tag="ACG81C_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066183.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1846..2505
                     /gene="vanR-B"
                     /locus_tag="ACG81C_003126"
     CDS             1846..2505
                     /gene="vanR-B"
                     /locus_tag="ACG81C_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS5066184.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2508..3851
                     /gene="vanS-B"
                     /locus_tag="ACG81C_003127"
     CDS             2508..3851
                     /gene="vanS-B"
                     /locus_tag="ACG81C_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS5066185.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4022..4828
                     /gene="vanY"
                     /locus_tag="ACG81C_003128"
     CDS             4022..4828
                     /gene="vanY"
                     /locus_tag="ACG81C_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5066186.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4846..5673
                     /gene="vanW-B"
                     /locus_tag="ACG81C_003129"
     CDS             4846..5673
                     /gene="vanW-B"
                     /locus_tag="ACG81C_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS5066187.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFGTDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5670..6641
                     /gene="vanH"
                     /locus_tag="ACG81C_003130"
     CDS             5670..6641
                     /gene="vanH"
                     /locus_tag="ACG81C_003130"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH"
                     /protein_id="HFS5066188.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGARVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6634..7662
                     /gene="vanB"
                     /locus_tag="ACG81C_003131"
     CDS             6634..7662
                     /gene="vanB"
                     /locus_tag="ACG81C_003131"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS5066189.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            7668..8276
                     /locus_tag="ACG81C_003132"
     CDS             7668..8276
                     /locus_tag="ACG81C_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS5066190.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(8342..8515)
                     /locus_tag="ACG81C_003133"
     CDS             complement(8342..8515)
                     /locus_tag="ACG81C_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066191.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2450 a         1820 c         2230 g         2150 t
ORIGIN      
        1 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
       61 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
      121 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
      181 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
      241 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
      301 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
      361 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
      421 aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta ttttctctgc
      481 cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg cgccgaaagt
      541 ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta cattgtatat
      601 ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat
      661 tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg
      721 gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac
      781 tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt
      841 cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa
      901 tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt
      961 ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat
     1021 tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat
     1081 catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt
     1141 atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg
     1201 gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg
     1261 tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta
     1321 actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc
     1381 ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt
     1441 acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc
     1501 cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg
     1561 gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat
     1621 cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta
     1681 ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa
     1741 gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg
     1801 aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt
     1861 ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca
     1921 ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac
     1981 acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg
     2041 cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac
     2101 gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa gccattcaag
     2161 atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag
     2221 gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt
     2281 acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa
     2341 ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagggt
     2401 gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc
     2461 atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg gaaagaaaag
     2521 ggattttcat taaggttttt tcctatacga tcattgccct gctactgctt gtcggtgtaa
     2581 cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa ttacagcaaa
     2641 cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg cttgatattc
     2701 aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat attgaagata
     2761 aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt tttagacccg
     2821 actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa agcaaggcag
     2881 gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt gcgataatgg
     2941 ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag
     3001 ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc
     3061 gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc aggctgaaag
     3121 aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag gaaacacagc
     3181 gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct acaagcgttc
     3241 tgttggaggg aatgcttgaa aatatcggtg actacaaaga tcattctaag tatctgcgcg
     3301 aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg gagcttgtca
     3361 gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg cgcacggttg
     3421 ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag
     3481 atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag gcactatcca
     3541 atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg atatggagtg
     3601 agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac attgatgata
     3661 ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcaa agcagaaaaa
     3721 gtgggcgaag cggtttagga cttgccatcg tacaaaaaac gctggatgcc atgagcctcc
     3781 agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta ccgctcacat
     3841 caacattgta aatatttaaa acttaaatga ttttgaccga caggtataac cctgccggtc
     3901 tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag atgcgcttgc
     3961 cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg cattcgatca
     4021 aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata tgaaacaatc
     4081 cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt
     4141 tttggggcgg agactggctt ccgtattgga ggcaacacag ataccgccca tccctgcaac
     4201 tcatacaggc agcagcactg acgtagtgga gaatttggag gaaaacgctc ttgccaccgc
     4261 caaagaacag ggagatgaac aggaatggag cctgatttta gtgaacaggc agaaccccat
     4321 ccccgcacag tacgatgtgg agcttgagca actatcaaat ggtgagcgga tagatattcg
     4381 gatttctccc tatcttcaag atttgtttga tgccgcaaga actgatggag tttacccgat
     4441 tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa attatggatg aaaaaattgc
     4501 cgaatataag gcgaaaggct acacctctgc acaggctaaa gcggaagcag aaacttgggt
     4561 ggccgtgccg ggaacgagcg agcatcagct tggtcttgct gtggatatca atgcggacgg
     4621 aattcattca acaggcaacg aggtttatag atggctggat gaaaacagct atcgttttgg
     4681 ttttattcgt cgctacccac cagacaagac agagataacc ggtgtgagca acgagccgtg
     4741 gcattaccga tatgttggca tcgaagctgc cacagagatg tacaaccaag gggtttgcct
     4801 tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga cagaaaaaga
     4861 ttgacacagc gtttcccgtt cctgcttcca atgagacggg cgcagagaaa aatgtgcttt
     4921 tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga cgatagggga aaaatcgctt
     4981 tcccatttgc tctttggaac ggattgcgca ttatacaacc acaataccgg atttgacatg
     5041 atataccagg aaaacaaggt gttcaactta aagctggcag caaagacctt aaacggctta
     5101 ttgataagac cgggggaacc cttttctttc tggtggctgg tacgccatgc ggacaaagat
     5161 accccctata aagacggcct tacggtgacc aatggtaaac tcaccaccat gtcgggcggc
     5221 ggtatgtgcc agatgagcaa tttactattt tggatgttcc tgcatacgcc attgacaatt
     5281 atccagcgca gaggtcacga agtaaaggag tttccagagc caaacagtga cgagattaaa
     5341 ggggtggatg caaccatttc agagggctgg attgatttaa aagtgcgaaa cgataccgac
     5401 tgcacctacc aaatatgggt gaccttagat gatgagaaaa tcatcggtca ggtgtccgcc
     5461 gacaaagagc cccaagcatt atacaaaatt acaaatggca gtatccagta tgtccgtgaa
     5521 agtggcggga tttatgaata tgcccaggtt aaacggatgc aagttgcctt aggtaccggg
     5581 gaaataatag attgcaagct gctttataca aacaaatgca aaatctgtta tcccctcccg
     5641 gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag tgtgggcatt actgtttttg
     5701 gatgcgaaca ggatgaggca aatgctttcc gcgctttatc gccggatttt catattatcc
     5761 ctacgctgat tagcgatgcg atatcggcag acaacgcaaa attggccgct ggcaatcaat
     5821 gcgttagcgt aggccataag tccgaggttt ccgaggcgac aattcttgcg ctgagaaagg
     5881 tcggggtaaa atacatttct acccgcagca tcggctgcga tcacattgat acgactgccg
     5941 ccgagagaat gggaatctcg gttggcacgg ttgcgtattc gccggacagc gttgcggatt
     6001 atgctttgat gctgatgctg atggccatac ggggtgcaaa acccaccatg cacgccgtgg
     6061 cgcaacaaga tttcagattg gatcgtatcc gggggaaaga actgggggat atgactgtgg
     6121 gagttattgg aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg ggatttggat
     6181 gccatgtgct ggcctatgat aacagccgaa aaatggatgc agattatgtc cagcttgatg
     6241 agcttctaaa aaacagcgat attgttacgc tccatgtgcc gctttgtgcg gatacccgcc
     6301 atctgatcgg tcagaagcaa attggagaga tgaagcaagg cgcattttta atcaacaccg
     6361 ggcgcggagc gcgtgtcgat accggggcgc tggtggaggc gttggaaagc ggaaagctgg
     6421 gtggtgcggc actggatgtg ttggagggcg aggatcagtt tgtttatacc gattgctcgc
     6481 agaaagtgct tgaccatccc tttttgtcgc agcttttaag gatgccgaat gtgatcatca
     6541 caccccatac ggcgtactac actgagcgtg tgctgcagga tactacagaa aaaacaatca
     6601 ggaattgtct caattttgaa aggagtttac agcatgaata gaataaaagt cgcaatcatc
     6661 ttcggcggtt gctcggagga acatgatgtg tcggtaaaat ccgcaataga aattgctgcg
     6721 aacattgata cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa cggtgtatgg
     6781 aagctatgca agaagccatg tacggaatgg gaagccgaca gtctccccgc catactctcc
     6841 ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga aacacggcgt
     6901 attgatgtgg ctttcccggt tttgcatggc aaatgcgggg aggatggtgc gatacagggg
     6961 ctgtttgtat tgtctggtat cccctatgtg ggctgtgata ttcaaagctc cgcagcttgc
     7021 atggacaaat cactggccta cattcttaca aaaaatgcgg gcatcgccgt tcccgaattt
     7081 caaatgattg ataaaggtga caagccggag gcgggtgcgc ttacctaccc tgtctttgtg
     7141 aagccggcac ggtcaggttc gtcctttggc gtaaccaaag taaacggtac ggaagaactt
     7201 aacgctgcga tagaagcggc aggacaatat gatggaaaaa tcttaattga gcaagcgatt
     7261 tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg atgatttgat tgtcggcgaa
     7321 gtggatcaaa tccggctgag ccacggtacc ttccgcatcc atcaggaaaa cgagccggaa
     7381 aaaggctcag aaaatgcgat gattacagtt cccgcagaca ttccggtcga ggaacgaaat
     7441 cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg gcttgcccgt
     7501 gttgatcttt ttttgcagga ggatggcggc atcgttctaa atgaggtcaa taccctgccc
     7561 ggttttacat cgtacagccg ctacccacgt atgatggccg ccgcaggaat cacgcttcct
     7621 gcactgattg acagcctgat tacattggcg ttaaagaggt gataagtatg gaaaatggtt
     7681 ttttgttttt agatgaaatg ttgcatggcg tccgttggga tgccaagtac gccacatggg
     7741 ataacttcac aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc ggcacaaagg
     7801 ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc tatggcttgc
     7861 ttttatggga tggatatcgg ccaagaactg cggtggactg cttcctgcgt tgggcagcgc
     7921 aaccggagga caatctcaca aaagaaaaat tttaccccaa tatagagcga gccgagttga
     7981 ttacaaaggg ttatgtggcc tcacaatcca gccatagccg tggaagcgca attgatctta
     8041 cgctctacca cctggataca ggggaacttg tttcaatggg aagtaacttc gattttatgg
     8101 acgaacggtc gcatcataca gcaaaaggga taggggatgc agaggcacaa aatcgaagat
     8161 gcttgcgtaa aatcatggaa agcagcggat ttcagtctta tcgctttgaa tggtggcact
     8221 ataagttgat tgatgagcca taccccgata cctattttaa ttttgccgtt tcataatgaa
     8281 agtattggtt ttgtatcaga ttttggattt gataaaatct atgctggtca tggacaatct
     8341 tttactggaa agtggttaaa gcatcttttg taatacattg caaattatag tattcaatct
     8401 gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg cccattttgt
     8461 attttgctgg cggcggtttt gagaaatcaa ggccgccgtt cttttctggc agcaaaaggt
     8521 gtggcggccc ataggaggat gccgctatgc tgttttgcta ttgccatgct ctaatccact
     8581 tccaccactc aaaaaaagcc ttgcccctcc atgaactgaa ccccaaaagt tgaactattt
     8641 aatggactgt
//
LOCUS       DAZUMI010000322         8650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.123, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000322 DAZUMI010000000
VERSION     DAZUMI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.123"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..573)
                     /locus_tag="ACG81C_003134"
     CDS             complement(202..573)
                     /locus_tag="ACG81C_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5066192.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            718..903
                     /locus_tag="ACG81C_003135"
     CDS             718..903
                     /locus_tag="ACG81C_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5066193.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1061..1228
                     /locus_tag="ACG81C_003136"
     CDS             1061..1228
                     /locus_tag="ACG81C_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066194.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1846..2505
                     /gene="vanR-B"
                     /locus_tag="ACG81C_003137"
     CDS             1846..2505
                     /gene="vanR-B"
                     /locus_tag="ACG81C_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS5066195.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2508..3851
                     /gene="vanS-B"
                     /locus_tag="ACG81C_003138"
     CDS             2508..3851
                     /gene="vanS-B"
                     /locus_tag="ACG81C_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS5066196.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4022..4828
                     /gene="vanY"
                     /locus_tag="ACG81C_003139"
     CDS             4022..4828
                     /gene="vanY"
                     /locus_tag="ACG81C_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5066197.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4846..5673
                     /gene="vanW-B"
                     /locus_tag="ACG81C_003140"
     CDS             4846..5673
                     /gene="vanW-B"
                     /locus_tag="ACG81C_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS5066198.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFGTDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5670..6641
                     /locus_tag="ACG81C_003141"
     CDS             5670..6641
                     /locus_tag="ACG81C_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS5066199.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6634..7662
                     /gene="vanB"
                     /locus_tag="ACG81C_003142"
     CDS             6634..7662
                     /gene="vanB"
                     /locus_tag="ACG81C_003142"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS5066200.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            7668..8276
                     /locus_tag="ACG81C_003143"
     CDS             7668..8276
                     /locus_tag="ACG81C_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS5066201.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(8342..8515)
                     /locus_tag="ACG81C_003144"
     CDS             complement(8342..8515)
                     /locus_tag="ACG81C_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066202.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2450 a         1820 c         2229 g         2151 t
ORIGIN      
        1 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
       61 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
      121 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
      181 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
      241 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
      301 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
      361 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
      421 aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta ttttctctgc
      481 cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg cgccgaaagt
      541 ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta cattgtatat
      601 ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat
      661 tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg
      721 gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac
      781 tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt
      841 cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa
      901 tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt
      961 ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat
     1021 tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat
     1081 catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt
     1141 atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg
     1201 gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg
     1261 tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta
     1321 actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc
     1381 ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt
     1441 acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc
     1501 cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg
     1561 gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat
     1621 cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta
     1681 ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa
     1741 gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg
     1801 aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt
     1861 ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca
     1921 ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac
     1981 acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg
     2041 cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac
     2101 gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa gccattcaag
     2161 atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag
     2221 gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt
     2281 acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa
     2341 ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagggt
     2401 gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc
     2461 atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg gaaagaaaag
     2521 ggattttcat taaggttttt tcctatacga tcattgccct gctactgctt gtcggtgtaa
     2581 cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa ttacagcaaa
     2641 cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg cttgatattc
     2701 aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat attgaagata
     2761 aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt tttagacccg
     2821 actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa agcaaggcag
     2881 gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt gcgataatgg
     2941 ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag
     3001 ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc
     3061 gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc aggctgaaag
     3121 aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag gaaacacagc
     3181 gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct acaagcgttc
     3241 tgttggaggg aatgcttgaa aatatcggtg actacaaaga tcattctaag tatctgcgcg
     3301 aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg gagcttgtca
     3361 gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg cgcacggttg
     3421 ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag
     3481 atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag gcactatcca
     3541 atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg atatggagtg
     3601 agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac attgatgata
     3661 ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcaa agcagaaaaa
     3721 gtgggcgaag cggtttagga cttgccatcg tacaaaaaac gctggatgcc atgagcctcc
     3781 agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta ccgctcacat
     3841 caacattgta aatatttaaa acttaaatga ttttgaccga caggtataac cctgccggtc
     3901 tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag atgcgcttgc
     3961 cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg cattcgatca
     4021 aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata tgaaacaatc
     4081 cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt
     4141 tttggggcgg agactggctt ccgtattgga ggcaacacag ataccgccca tccctgcaac
     4201 tcatacaggc agcagcactg acgtagtgga gaatttggag gaaaacgctc ttgccaccgc
     4261 caaagaacag ggagatgaac aggaatggag cctgatttta gtgaacaggc agaaccccat
     4321 ccccgcacag tacgatgtgg agcttgagca actatcaaat ggtgagcgga tagatattcg
     4381 gatttctccc tatcttcaag atttgtttga tgccgcaaga actgatggag tttacccgat
     4441 tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa attatggatg aaaaaattgc
     4501 cgaatataag gcgaaaggct acacctctgc acaggctaaa gcggaagcag aaacttgggt
     4561 ggccgtgccg ggaacgagcg agcatcagct tggtcttgct gtggatatca atgcggacgg
     4621 aattcattca acaggcaacg aggtttatag atggctggat gaaaacagct atcgttttgg
     4681 ttttattcgt cgctacccac cagacaagac agagataacc ggtgtgagca acgagccgtg
     4741 gcattaccga tatgttggca tcgaagctgc cacagagatg tacaaccaag gggtttgcct
     4801 tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga cagaaaaaga
     4861 ttgacacagc gtttcccgtt cctgcttcca atgagacggg cgcagagaaa aatgtgcttt
     4921 tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga cgatagggga aaaatcgctt
     4981 tcccatttgc tctttggaac ggattgcgca ttatacaacc acaataccgg atttgacatg
     5041 atataccagg aaaacaaggt gttcaactta aagctggcag caaagacctt aaacggctta
     5101 ttgataagac cgggggaacc cttttctttc tggtggctgg tacgccatgc ggacaaagat
     5161 accccctata aagacggcct tacggtgacc aatggtaaac tcaccaccat gtcgggcggc
     5221 ggtatgtgcc agatgagcaa tttactattt tggatgttcc tgcatacgcc attgacaatt
     5281 atccagcgca gaggtcacga agtaaaggag tttccagagc caaacagtga cgagattaaa
     5341 ggggtggatg caaccatttc agagggctgg attgatttaa aagtgcgaaa cgataccgac
     5401 tgcacctacc aaatatgggt gaccttagat gatgagaaaa tcatcggtca ggtgtccgcc
     5461 gacaaagagc cccaagcatt atacaaaatt acaaatggca gtatccagta tgtccgtgaa
     5521 agtggcggga tttatgaata tgcccaggtt aaacggatgc aagttgcctt aggtaccggg
     5581 gaaataatag attgcaagct gctttataca aacaaatgca aaatctgtta tcccctcccg
     5641 gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag tgtgggcatt actgtttttg
     5701 gatgcgaaca ggatgaggca aatgctttcc gcgctttatc gccggatttt catattatcc
     5761 ctacgctgat tagcgatgcg atatcggcag acaacgcaaa attggccgct ggcaatcaat
     5821 gcgttagcgt aggccataag tccgaggttt ccgaggcgac aattcttgcg ctgagaaagg
     5881 tcggggtaaa atacatttct acccgcagca tcggctgcga tcacattgat acgactgccg
     5941 ccgagagaat gggaatctcg gttggcacgg ttgcgtattc gccggacagc gttgcggatt
     6001 atgctttgat gctgatgctg atggccatac ggggtgcaaa acccaccatg cacgccgtgg
     6061 cgcaacaaga tttcagattg gatcgtatcc gggggaaaga actgggggat atgactgtgg
     6121 gagttattgg aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg ggatttggat
     6181 gccatgtgct ggcctatgat aacagccgaa aaatggatgc agattatgtc cagcttgatg
     6241 agcttctaaa aaacagcgat attgttacgc tccatgtgcc gctttgtgcg gatacccgcc
     6301 atctgatcgg tcagaagcaa attggagaga tgaagcaagg cgcattttta atcaacaccg
     6361 ggcgcggagc gcttgtcgat accggggcgc tggtggaggc gttggaaagc ggaaagctgg
     6421 gtggtgcggc actggatgtg ttggagggcg aggatcagtt tgtttatacc gattgctcgc
     6481 agaaagtgct tgaccatccc tttttgtcgc agcttttaag gatgccgaat gtgatcatca
     6541 caccccatac ggcgtactac actgagcgtg tgctgcagga tactacagaa aaaacaatca
     6601 ggaattgtct caattttgaa aggagtttac agcatgaata gaataaaagt cgcaatcatc
     6661 ttcggcggtt gctcggagga acatgatgtg tcggtaaaat ccgcaataga aattgctgcg
     6721 aacattgata cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa cggtgtatgg
     6781 aagctatgca agaagccatg tacggaatgg gaagccgaca gtctccccgc catactctcc
     6841 ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga aacacggcgt
     6901 attgatgtgg ctttcccggt tttgcatggc aaatgcgggg aggatggtgc gatacagggg
     6961 ctgtttgtat tgtctggtat cccctatgtg ggctgtgata ttcaaagctc cgcagcttgc
     7021 atggacaaat cactggccta cattcttaca aaaaatgcgg gcatcgccgt tcccgaattt
     7081 caaatgattg ataaaggtga caagccggag gcgggtgcgc ttacctaccc tgtctttgtg
     7141 aagccggcac ggtcaggttc gtcctttggc gtaaccaaag taaacggtac ggaagaactt
     7201 aacgctgcga tagaagcggc aggacaatat gatggaaaaa tcttaattga gcaagcgatt
     7261 tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg atgatttgat tgtcggcgaa
     7321 gtggatcaaa tccggctgag ccacggtacc ttccgcatcc atcaggaaaa cgagccggaa
     7381 aaaggctcag aaaatgcgat gattacagtt cccgcagaca ttccggtcga ggaacgaaat
     7441 cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg gcttgcccgt
     7501 gttgatcttt ttttgcagga ggatggcggc atcgttctaa atgaggtcaa taccctgccc
     7561 ggttttacat cgtacagccg ctacccacgt atgatggccg ccgcaggaat cacgcttcct
     7621 gcactgattg acagcctgat tacattggcg ttaaagaggt gataagtatg gaaaatggtt
     7681 ttttgttttt agatgaaatg ttgcatggcg tccgttggga tgccaagtac gccacatggg
     7741 ataacttcac aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc ggcacaaagg
     7801 ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc tatggcttgc
     7861 ttttatggga tggatatcgg ccaagaactg cggtggactg cttcctgcgt tgggcagcgc
     7921 aaccggagga caatctcaca aaagaaaaat tttaccccaa tatagagcga gccgagttga
     7981 ttacaaaggg ttatgtggcc tcacaatcca gccatagccg tggaagcgca attgatctta
     8041 cgctctacca cctggataca ggggaacttg tttcaatggg aagtaacttc gattttatgg
     8101 acgaacggtc gcatcataca gcaaaaggga taggggatgc agaggcacaa aatcgaagat
     8161 gcttgcgtaa aatcatggaa agcagcggat ttcagtctta tcgctttgaa tggtggcact
     8221 ataagttgat tgatgagcca taccccgata cctattttaa ttttgccgtt tcataatgaa
     8281 agtattggtt ttgtatcaga ttttggattt gataaaatct atgctggtca tggacaatct
     8341 tttactggaa agtggttaaa gcatcttttg taatacattg caaattatag tattcaatct
     8401 gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg cccattttgt
     8461 attttgctgg cggcggtttt gagaaatcaa ggccgccgtt cttttctggc agcaaaaggt
     8521 gtggcggccc ataggaggat gccgctatgc tgttttgcta ttgccatgct ctaatccact
     8581 tccaccactc aaaaaaagcc ttgcccctcc atgaactgaa ccccaaaagt tgaactattt
     8641 aatggactgt
//
LOCUS       DAZUMI010000323         8141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.128, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000323 DAZUMI010000000
VERSION     DAZUMI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.128"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..86
                     /locus_tag="ACG81C_003145"
     CDS             <1..86
                     /locus_tag="ACG81C_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5066203.1"
                     /translation="MINPNSKEDMELLREVLRNNNFDDEDE"
     gene            222..314
                     /locus_tag="ACG81C_003146"
                     /pseudo
     CDS             222..314
                     /locus_tag="ACG81C_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            428..1063
                     /locus_tag="ACG81C_003147"
     CDS             428..1063
                     /locus_tag="ACG81C_003147"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5066204.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            1170..2408
                     /gene="mef(H)"
                     /locus_tag="ACG81C_003148"
                     /gene_synonym="mefH"
     CDS             1170..2408
                     /gene="mef(H)"
                     /locus_tag="ACG81C_003148"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS5066205.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2635..3054
                     /locus_tag="ACG81C_003149"
     CDS             2635..3054
                     /locus_tag="ACG81C_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5066206.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            3059..3298
                     /locus_tag="ACG81C_003150"
     CDS             3059..3298
                     /locus_tag="ACG81C_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5066207.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(3523..4446)
                     /locus_tag="ACG81C_003151"
     CDS             complement(3523..4446)
                     /locus_tag="ACG81C_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5066208.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4478..5242)
                     /gene="map"
                     /locus_tag="ACG81C_003152"
     CDS             complement(4478..5242)
                     /gene="map"
                     /locus_tag="ACG81C_003152"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5066209.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5400..5840
                     /locus_tag="ACG81C_003153"
     CDS             5400..5840
                     /locus_tag="ACG81C_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5066210.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(6018..6587)
                     /locus_tag="ACG81C_003154"
     CDS             complement(6018..6587)
                     /locus_tag="ACG81C_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5066211.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            6842..8074
                     /locus_tag="ACG81C_003155"
     CDS             6842..8074
                     /locus_tag="ACG81C_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5066212.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         2643 a         1397 c         1515 g         2586 t
ORIGIN      
        1 aaatgataaa cccgaactca aaagaagata tggaactgtt acgagaagta ttaaggaata
       61 acaattttga tgatgaggac gagtaacttt tttcctcttg cgagaagata cagcaaacaa
      121 aaagcccgat caagagtgca aagcaccacc aatggtggca aagctcttga cagggctttt
      181 tcttttctgt tgtgggcaat caagaggaag aaagggataa aatctgattt gtggtaaatc
      241 aggcaaaggc aacgcaggta tcacagtatg aattgacacc gcataaggaa agcaactgga
      301 agattgtaga ataaatgaaa aaggcttgca ttctgcattt ttataaaatt gcaagtcttt
      361 atattgacaa gcataaaata ctaagatata ctttaatcag accaacggtt tgtattggag
      421 gtaagttatg gcacgaaata aatatccaga agaaacagta aaactcattt tagatgtttc
      481 tacaaaaatg ttttgtgaaa aaggatatga taacacatca ttgcaagata ttatcaatga
      541 aacaaaactt tctaaaggtg ctatttatca tcatttcgat tcaaaggaag atattttaaa
      601 agcaattttt caaagaattg gaaatgaaaa tgcagtaata tttgctaaaa ttagagataa
      661 cgatagttta aacggtttag aaaagttaag agaaatattt aagacagcgg tattcaattc
      721 taaccaaagc gttttattga cagtttctcc tcaactatta aacaatcctc gttttcttgc
      781 tatgcaaata gaacaaatgt atgaaattgt agtacctgaa tttgttgaac ccgttttaga
      841 acaaggtatc aaggataagt ctttggagat tgaaaattca catgaaattg cagaagttat
      901 catggtatta agtaatgtat ggctcaaccc attagttgaa atgacggata aagagggtat
      961 ggaaaaacgt tgcaatacat tcaatgccct tttaaatgga ataggcatag acacattatt
     1021 agacgaagaa acaatttctg catttataca gttttgtaaa taactgtatt tgttatccct
     1081 atacgataga ctgctctata aaagagcagt ttatttaaac cattatataa accgacggtc
     1141 tgtttgatta aataaaaagg agagatttta tgcaagcaga aaaaaaactt tttaatcgtg
     1201 attttatact tgttgttata ggacagataa tttctttatt tggaaacgct atcctacgat
     1261 ttgcattacc gtactatttg ttaaaagaaa ctggttcatc agctttgttt ggtattgcta
     1321 cagcctgctc ttttttacct atggttattt tgtcgctggt aggtggtatt attgctgaca
     1381 ggacaaataa aagaaacatt atggtcatac tagattttgt aacagcaatc ataattttag
     1441 tattttattt gtcattggga aaaattccta tcataccttt gtttattgtt gctttgatgt
     1501 tactttatgg tatttctggt gcatatcaac cagcggtaca agcaagtatt cctctattgg
     1561 tatcatcaga taaacttacc gtcggtaatg ctataataaa tcaagtaaat actttagcaa
     1621 actttatcgg accagctata agcggagtag tttttcaatt ttatggtatc aatcccattt
     1681 taattgttag tattatatgt tttacttttt cagccattat ggaaattttt atccatattc
     1741 cacaccaaaa aagacctcgt gaagtaggtg taattgcagt tgctaaaaac gatttaaaag
     1801 atagttatac ctttttgaaa aaggagaaac ccatttttat atccgtaatc tttttggttt
     1861 gcattttcaa tttggtttta agtgctgttt taattgttgg tctgcctatc ttaatttctg
     1921 aagttttaaa aatgccagat acactatatg gtttttcaca atcagcatta tcattaggtg
     1981 gtttatgtgg tggtatttta acagctcttg ttgcttcaaa gttaaaacta aaaaaccttt
     2041 ataccttgtt acttatttgt tctgtttctg ttttacttat gggaatttcg ctatttttag
     2101 aattgcctgc aatagtttcc tactgggtag ttacagtgat gtgctttgtg gctatgggtg
     2161 tatctactat gtttgttgtt caaatttaca ctcttgttca gacacaaaca ccaccgcaat
     2221 tagtaggaaa gataatggct acgctggtag cagttgctat gtgcggtcaa cctattggac
     2281 aaatggttta cggtatttta ttcgatgtat ttatacaaga aacatgggtt gttatgtttg
     2341 tagcttctat tgcttcaatc atgatttcaa tatattcaaa aagaattttt caaaagttag
     2401 agatataata caaggggtta aatagtatgt taggctaaaa acaatatata aaagaaaatt
     2461 tgaaatctgc taaaaggata gtgttataaa agaaagagta atattgcagt atttaaggct
     2521 cgcacaaata tatgttttgt gtgggctttt ttcttaccat ttttctgtat ttctaaaggg
     2581 tataaacggt attccgttta tatatttcag cattagcaga aagggggtga gattatgagg
     2641 acatcttctt tcgagcatat cgtcagatta cagttcaatt ctttaatgct gattgtcatt
     2701 aagaacaaag tgaaaagtcg aaacagacag cttgcaagac attctaaacg tgaagtattg
     2761 ttttgtgaaa tagccgaaac gaaacaagtt gaacgtggaa cgattgatac ctgtttttat
     2821 gattgtgtat catttaaagt cttgcacttt acaatttatg tatctgatga aacacttggt
     2881 acagttttac atagactgtc agaaaaacag cgtagtgcga ttttattacg ttattttcaa
     2941 ggaatgaacg acagagaaat ttcagaactg taccatgtat cacgttctgc tatttgcagt
     3001 agaagaacta gaggactaag gaagttaaaa atgcttttga gcgaaaggaa gtaaagaaat
     3061 gagtaaagat tatggctatc cctcttttga attgatttgc aaagcgtcaa gcggagatga
     3121 aatggcagtt aaagagattt tgaagtttta tgacggttat atttctaaat tatgcttgcg
     3181 tcctttttac cactctgaaa gtggtaaaat cattatgcag gttgatgaag aactaaaagg
     3241 agaaattcat acagacatga tgaaagcaat attgaaattt gaaatcggag tgaagtaatc
     3301 aactgtataa aaaaatcggg agatacacag tttcgtgcta tctcccttag tagttttacg
     3361 gtcatatcaa ggctcattca atcgtggtaa attcgtggta agatgagtgt ttttctatac
     3421 ttcaaaatgc ttgaatgtat agtgtttatg cggataattg aaaattagat ataaaatttc
     3481 aaataaatta ctgatataaa tttacaataa gaactcttgt ttctactttt ttttatgaga
     3541 aaatttagtt ttcaatttat tgaaccattt tcggcttgga tctcgacgtt ttttgatttg
     3601 atgacccgtc acgtattctt gaacaactgc attgatgatt ccgcccaaaa tgatgatcgt
     3661 agcagcaaaa ttaagccaaa gcatgagtac aataaaactt cctatgatct gataaccgct
     3721 gatcctcgtt gcaaaatacc tagcatagat actaaacact tgtgataaca gcatccagcc
     3781 gacagtagcg aagatcgtgc ccggaatgat cgtcttcaac ttcaactgag cattagggac
     3841 aattaaataa atcaagaaca tgatgacaaa taaagctacc aatgtgattg gccacttcaa
     3901 agtttggaac tgatcgataa agttcacggg tatttgtacg attggctgaa tagcatccaa
     3961 aatcagctgt cccaacccaa gaacgatagt taccccacta atagcaatca tgaacaagac
     4021 aatgacaatt agtgagaaga aacggacgat caaaaaattt tttctatttt ctacgccata
     4081 cgctttattc atcgcaattt gcaaagcatt gatactttga cttgcagacc ataaagccgc
     4141 taatgcagaa acggataaca agcctcctga actttgtgtc aaaaggtctt tgattgcagg
     4201 accgataaac tggtaaacgg tctcaggaat cacttctcgt aaataggtca gtacagtctc
     4261 ttgatcgatg tggagaaacg gtaaaatatt accaaaagca ataatcaatg gaaataaaga
     4321 caagagtaaa taataagcaa cgacgacaga ggtcgtactc atttctgaat cagtgatgcg
     4381 cttttgagtc gtctcaataa agcgcatcag attttcattt tgttttattt tgttccaaac
     4441 tttcatgtac ctctcccgta cctattgact ggatagatca ataagtgcct tcttctcctt
     4501 gtgatgtcaa aattcttgga ccatctttag taatagccaa tgtatgttca tattggcaag
     4561 aaagtccgcc gtctcttgta tacgcagtcc agccgttagg atccattttc attttccaag
     4621 ttccggtatt aaccataggt tcgatcgtga taaccatgcc ttctttcaac cgcaaccctt
     4681 ttccagcttc tccgtaatga ggaactgctg gttcttcatg aatcgtagga ccaacgccat
     4741 gaccaataaa atcacgaacg atcgctagat tttcgccttc tacataagtt tggatagcat
     4801 gaccgatgtc accaatccga ttccctactt gtgcttgctc aataccgatg tacaaagctt
     4861 tcttcgttac ttccatcaat cggtcgatct caggtgtaga ttctccaact acataggccc
     4921 aacaagaatc agaaatgccg cctttcaaat cgatacacat atcgactttt accaaatcac
     4981 cgtttttcaa aggcttttta cgagggaatc catgacaaat ttcatcattt atactgatac
     5041 aagtcgcata ttcataccct tcaaagccaa tttgcgcagc aattccgcca tgactctcga
     5101 tatagtttct gacaaatacc tcaatatccc aacttgtgat tcctggtttt ataaaatcac
     5161 gcagattttt atgtacatct gctaataaag ctccagattc tgccatcatt tcaatttcac
     5221 gtgctgattt taaagtaatc atattttcgt ctccttttta caaatccctc tcattttaac
     5281 atagcggcat acatttagcc aaatgacatt ccccaggtgt ctctaggttt tattttcttt
     5341 tcaatgatgt tttggtataa ttcataattg ttaaataatt ataaacgagg aggcacatca
     5401 tggctttagc aaaaatcgta tatgctagca tgacaggaaa cactgaagaa atcgccgata
     5461 ttgttgcaga agcatttgaa aatctggata tcgaagtcga aatcaacgaa tgtacccaag
     5521 tagatgcaga tgaattcgag gaggcagata tttgcgtagt agctacttat acatacgatg
     5581 atggcgatct gcctgatgag atcgtagact tttatgaaga cttgcaagaa ttggatctat
     5641 tgggaaaaat ttacggtgtc tgcggatctg gtgatacttt ttacgatgaa ttctgcaaat
     5701 cggtggatga tttcgatgct gcttttgcaa aaacaggcgc atcaaaagga gccgaaaatg
     5761 ttaaagtgga tctgaatgct gaagaagaag atatcgaaaa tttagaagct ttcgctaaag
     5821 aactagtcgc aaaaatttaa tatacttgca ataaaagagc catccagatt tattcaatct
     5881 gtatggctct tttattcatt gcattcatgg aagagttctt cgggagttaa aaatgcatac
     5941 gctgtaagaa aaacgaactt ttccttattt tgacacatct cttgttacct actgatttgt
     6001 ggaacttttg agtatattca atcgatttcg attggttctt tgaatttcaa gatagcatct
     6061 atactgtctt ggatcatctt ttcaatctgt tcatcagttg gatggatgat gcttgaagac
     6121 atcaaagctg cgatcccagt agctatgatc catgtcccat tatacaaaga ttcgatctgc
     6181 tcttctgaaa gattcacata agctggatcg cgtttcactg tattcacaaa gtaagtataa
     6241 gagaaattct gcatctcttt tcctccacca tattcttcta agtacagtga acgatacaac
     6301 ttcttctctc ttcttgcaaa atggatataa ttaaaagcaa gatcgataat cgtattccct
     6361 gtatgctcaa cagtgaacac ttcatattgt aaatgatcat agatctgctc aaaaagagct
     6421 tgtctcaagt catccatatt tttaaactca agataaatcg gttgcgtgga acaattcatt
     6481 ttgttcgcga tgtttctcgc agtaaatcgt gtaaatccat cggtggcagc tacttcataa
     6541 gcagctgcca aaatctgttc tctagtaatc gtcttttttc ttgccatatt ttttaatctc
     6601 cttaattttt ttataacgct tccaccattg attaatttca ttgtaacatg ttttttttga
     6661 tatgcaaaat tagtttttga ttttcttcac aagctgaaat agcaagtagt aacatatatc
     6721 atgaaactta accttaaaaa aataacctcc tttttttatt taaagcctca ttgtttgaat
     6781 cataattaaa tggtatcatg ctaatagtta ccagaaaaaa tggtgaggag gaatttttat
     6841 tatgttatct gattttaatg aacttctaaa aaaatatgca cgcttaattg cagaaactgg
     6901 cgtagccaca gaaaaaggac acacagttgt tttacagatc agcgtggaac aggcacccct
     6961 tgctcgattg atcacacaag aagcttataa actaggagcc gctgaggtta tcgttcaatg
     7021 gactgacgac cagatccaaa gagaatttct tttacatgct gcaacggatc gtattgagaa
     7081 tgtaccacaa tctaaaatcg atcaagcgga tgaatggctt gaaaaaggag caagcaggat
     7141 cagcgttgtt tctgccgatc ccgatgcatt tgctggtgta gatagccatc gagtagcgtc
     7201 ttatcaatca gcgaatggca aagcactaat gaatttacgc aaggcaactc aagcaaataa
     7261 agtcagttgg actgtcgtgg ctgcggctgg taaacaatgg gcagcaaaag tcttcccaga
     7321 actgccagaa gaaaaacaag ttgaggcatt atgggatcag attttcaaaa cgactcgtgt
     7381 ctatgaagaa gaccctgtct tagcatggaa gaagcatgat gaaaaattag caaaaaaagc
     7441 agaagaattg aatcgagaac aattttctgc ccttcactat actgctcccg gtacagatat
     7501 cattatcgga ttgcctaaaa accatctttg ggaaggcgct ggaagctata atgctcgtgg
     7561 agaaaaattc atggcaaata tgccgacgga agaagtgttc acagcacctg acagtcatcg
     7621 agtagatggt tacatttcaa gtacaaagcc actgagttat gctggcacca tcatctcggg
     7681 tatgaaattt acttttaaag atggaaaagt tgttgatttt tctgccgaac aaggagaaga
     7741 tgtcttagca aaactgcttg atacagatga aggtgcgcgt cgtttaggag aagtcgcact
     7801 tgtacctgac ccttcaccta tttctcaatc tggcatcatc tttttcaata cattatttga
     7861 tgaaaacgca tcgaatcact tagcactagg ttctgcttat gccttcagtg tgaaaggcgg
     7921 tacagaaatg tccgatgaag aattagcaga agctggattg aatagaagtc aaacccacgt
     7981 agatttcatg gtaggatctg acaagatgga tatcgacgga atccgtgaag atggctctac
     8041 agtaccgatt ttccgaaatg gcgattgggc ttgagtatac tttcgcacac atgcaaaaaa
     8101 taatggctct ttgtcaataa ggactgatga gttgtgcaaa a
//
LOCUS       DAZUMI010000324         7958 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000324 DAZUMI010000000
VERSION     DAZUMI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.132"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG81C_003156"
     CDS             128..1564
                     /locus_tag="ACG81C_003156"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5066213.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG81C_003157"
     CDS             1753..4200
                     /locus_tag="ACG81C_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5066214.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG81C_003158"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG81C_003158"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5066215.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG81C_003159"
     CDS             5487..5693
                     /locus_tag="ACG81C_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066216.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG81C_003160"
     CDS             complement(5690..6793)
                     /locus_tag="ACG81C_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5066217.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG81C_003161"
     CDS             7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG81C_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5066218.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat agagatactg
     7261 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     7321 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     7381 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     7441 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     7501 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     7561 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     7621 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     7681 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     7741 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     7801 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     7861 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     7921 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DAZUMI010000325         5474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.162, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000325 DAZUMI010000000
VERSION     DAZUMI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.162"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1014
                     /locus_tag="ACG81C_003162"
     CDS             322..1014
                     /locus_tag="ACG81C_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5066219.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG81C_003163"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG81C_003163"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5066220.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="ACG81C_003164"
     CDS             complement(1910..3862)
                     /locus_tag="ACG81C_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5066221.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="ACG81C_003165"
     CDS             complement(3869..4105)
                     /locus_tag="ACG81C_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066222.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="ACG81C_003166"
     CDS             4507..4797
                     /locus_tag="ACG81C_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5066223.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(5069..5418)
                     /locus_tag="ACG81C_003167"
                     /pseudo
     CDS             complement(5069..5418)
                     /locus_tag="ACG81C_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1857 a          887 c          942 g         1788 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgt
//
LOCUS       DAZUMI010000326         2772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.214, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000326 DAZUMI010000000
VERSION     DAZUMI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.214"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG81C_003168"
     CDS             111..194
                     /locus_tag="ACG81C_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5066224.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG81C_003169"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG81C_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5066225.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACG81C_003170"
     CDS             complement(1319..2182)
                     /locus_tag="ACG81C_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5066226.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACG81C_003171"
     CDS             complement(2184..2456)
                     /locus_tag="ACG81C_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5066227.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACG81C_003172"
     CDS             complement(2473..2682)
                     /locus_tag="ACG81C_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5066228.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAZUMI010000327         1573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5075
            SAMEA115707478-rid22580353.guided.246, whole genome shotgun
            sequence.
ACCESSION   DAZUMI010000327 DAZUMI010000000
VERSION     DAZUMI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707478
            Sequence Read Archive: ERR13197207
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288037.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288037.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:06:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,115
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 48 of 86
            Pseudo Genes (internal stop)      :: 20 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707478-rid22580353.guided.246"
                     /isolate="VRE5075"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1143
                     /locus_tag="ACG81C_003173"
                     /note="aph(2'')-Ia"
     CDS             <1..1143
                     /locus_tag="ACG81C_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS5066229.1"
                     /translation="KGFYSLFKTLQQNRTRYIKLIFEFLKKERNANAVILDPHKNNPR
                     AIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFK
                     VDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPN
                     IEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDI
                     SECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDF
                     SCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNID
                     IEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1273..>1573)
                     /locus_tag="ACG81C_003174"
     CDS             complement(1273..>1573)
                     /locus_tag="ACG81C_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5066230.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          624 a          150 c          265 g          534 t
ORIGIN      
        1 aagggatttt attctttatt taaaactttg caacagaacc gtacaagata tattaaattg
       61 atttttgaat ttttgaaaaa agaaagaaat gctaatgcag ttattttaga ccctcataaa
      121 aataatccaa gagcaataag ggcataccaa aaatctggtt ttagaattat tgaagatttg
      181 ccagaacatg aattacacga gggcaaaaaa gaagattgtt atttaatgga atatagatat
      241 gatgataatg ccacaaatgt taaggcaatg aaatatttaa ttgagcatta ctttgataat
      301 ttcaaagtag atagtattga aataatcggt agtggttatg atagtgtggc atatttagtt
      361 aataatgaat acatttttaa aacaaaattt agtactaata agaaaaaagg ttatgcaaaa
      421 gaaaaagcaa tatataattt tttaaataca aatttagaaa ctaatgtaaa aattcctaat
      481 attgaatatt cgtatattag tgatgaatta tctatactag gttataaaga aattaaagga
      541 acttttttaa caccagaaat ttattctact atgtcagaag aagaacaaaa tttgttaaaa
      601 cgagatattg ccagtttttt aagacaaatg cacggtttag attatacaga tattagtgaa
      661 tgtactattg ataataaaca aaatgtatta gaagagtata tattgttgcg tgaaactatt
      721 tataatgatt taactgatat agaaaaagat tatatagaaa gttttatgga aagactaaat
      781 gcaacaacag tttttgaggg taaaaagtgt ttatgccata atgattttag ttgtaatcat
      841 ctattgttag atggcaataa tagattaact ggaataattg attttggaga ttctggaatt
      901 atagatgaat attgtgattt tatatactta cttgaagata gtgaagaaga aataggaaca
      961 aattttggag aagatatatt aagaatgtat ggaaatatag atattgagaa agcaaaagaa
     1021 tatcaagata tagttgaaga atattatcct attgaaacta ttgtttatgg aattaaaaat
     1081 attaaacagg aatttatcga aaatggtaga aaagaaattt ataaaaggac ttataaagat
     1141 tgattatata atatatgaaa agctattata aaagacatta gtattaaata gtttaaaaaa
     1201 atgaaaaata ataaaggaag tgagtcaatt ccagactcct gtgtaaaatg ctatacaatg
     1261 tttttaccat ttctacttat caaaattgat gtattttctt gaagaataaa tccattcatc
     1321 atgtaggtcc ataagaacgg ctccaattaa gcgattggct gatgtttgat tggggaagat
     1381 gcgaataatc ttttctcttc tgcgtacttc ttgattcagt cgttcaatta gattggtact
     1441 ctttagtcga ttgtgggaat ttccttgtac ggtatattga aaggcgtctt cgaatccatc
     1501 atccaatgat gcgcaagctt ttgaatattt tggttggttc tgttgcaaag ttttaaatct
     1561 actatcaaat aag
//
